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采用高效液相色谱法同时测定磺胺二甲嘧啶和泰乐菌素组分含量,采用C18(4.6×250 mm,5μm)色谱柱;以2 mol/L高氯酸钠溶液-乙腈为流动相进行梯度洗脱;流速为1.0 m L/min;检测波长为280 nm。结果表明磺胺二甲嘧啶在10~200μg/m L范围内具有良好的线性,相关系数为0.9997,检出限为0.05μg/m L,定量限为0.15μg/m L,低、中、高3个浓度加标的平均回收率为99.7%~100.1%,RSD为0.3%~0.5%,泰乐菌素D峰与A峰的分离度≥3.2;泰乐菌素C峰与磺胺二甲嘧啶分离度≥30,该方法操作简便、准确、专属性强。 相似文献
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磺胺二甲嘧啶是畜禽生产中常用的抗菌药物,但在饲料中长期添加或滥用可导致动物性食品中的药物残留,人食用含有磺胺二甲嘧啶残留的动物性食品后,会引起再生障碍性贫血,并有致癌等毒副作用。欧美各国和我国均将其列为兽药残留监控的重点。磺胺二甲嘧啶检测常用的有气相色谱一串联质谱法和高效液相色谱法等,由于对检测设备、环境条件、操作技能要求高,同时受检测周期长:小批量检测成本高等因素的限制,在基层推广应用较为困难。磺胺二甲嘧啶检测快速筛选酶联免疫法(ELLSA方法)是利用抗原与抗体的特异性免疫化学反应的基本原理进行的。样品前处理简单,检测时间短,可同时检测多个样品。 相似文献
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农业部于 2 0 0 3年 1月 2 2日发布 2 3 6号公告 ,发布 1 2个动物性食品中兽药残留检测方法。方法之三 :动物性食品中磺胺二甲嘧啶残留检测方法—高效液相色谱法。 相似文献
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气相色谱-串联质谱法检测猪肉组织中的磺胺二甲嘧啶残留量 总被引:2,自引:0,他引:2
利用气相色谱-串联质谱法分析猪肉组织中的磺胺二甲嘧啶残留量。样品脱脂后,采用二氯甲烷提取样品中的目标化合物,经萃取、硅烷化衍生后,用气相色谱-串联质谱法检测,根据保留时间和质谱图谱与对照品比较进行定性与确认。所检测的磺胺二甲嘧啶的二级质谱图与标准的二级质谱图匹配良好,方法快速准确,干扰较少。该法测定磺胺二甲嘧啶的回收率为76%~110%,检出限为0.01mg/kg,适用于磺胺二甲嘧啶残留量的检测。利用气相色谱-串联质谱法分析猪肉组织中的磺胺二甲嘧啶残留量。样品脱脂后,采用二氯甲烷提取样品中的目标化合物,经萃取、硅烷化衍生后,用气相色谱-串联质谱法检测,根据保留时间和质谱图谱与对照品比较进行定性与确认。所检测的磺胺二甲嘧啶的二级质谱图与标准的二级质谱图匹配良好,方法快速准确,干扰较少。该法测定磺胺二甲嘧啶的回收率为76%~110%,检出限为0.01mg/kg,适用于磺胺二甲嘧啶残留量的检测。 相似文献
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用添加临床剂量100 mg/kg磺胺二甲嘧啶的饲料喂养40日龄麻黄鸡,连续用药3 d,分别在停药0、12、24、48、72、96、120、144、192、240、360、480、600 h时间点对肌肉、肝和肾组织采样,检测磺胺二甲嘧啶残留浓度。停药25 d后,肌肉组织残留量为0.054 mg/kg,肝和肾组织未检出磺胺二甲嘧啶,结果表明磺胺二甲嘧啶在麻黄鸡组织中停药期为25 d,《中国兽药典》2005年版中规定磺胺二甲嘧啶10 d的停药期是不尽合理的。 相似文献
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磺胺二甲嘧啶与甲氧苄啶以5∶1配伍制成复方磺胺二甲嘧啶片,治疗仔猪白痢,内服,用量25mg/kg·次(按磺胺二甲嘧啶计),每日2次,有效率为92.9%。 相似文献
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鱼肌肉中磺胺二甲嘧啶及其代谢产物的高效液相色谱法测定 总被引:3,自引:0,他引:3
研究了鲟鱼肌肉中磺胺二甲嘧啶及其主要代谢物N4 乙酰化磺胺二甲嘧啶的高效液相色谱测定法。样品用乙腈提取,正己烷脱脂,碱性氧化铝SPE柱净化,高效液相色谱 Inertsil ODS 3(4 6 mm×250 mm, 粒径 5μm),流动相:水 乙腈 乙酸(76∶24∶0 05, V/V/V)测定。在 20、100、2 000μg/kg 的添加浓度,测得 SM2、N4 ACE SM2 的平均回收率范围分别为80 4%~85.3%、88.4%~96.0%;2种药物的检测限均为10μg/kg。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献