优质多抗辣椒新品种濮优602的选育

辣椒濮优602系濮阳市农科院以H0616自交系为母本,自交系黄-2-6为父本杂交而成的一代杂种。早熟,始花节位8.6节;生长势强,连续坐果性好,株高65.6 cm,株幅66.3 cm;果实长羊角形,果实纵径18~23 cm,横径3.9 cm,果肉厚0.31 cm,单果质量90 g左右;青熟果黄绿色,果面微皱;每100 g鲜果含VC 85.2 mg,可溶性总糖含量为2.38%,蛋白质含量为1.50%,辣椒素含量7.43 mg/kg,肉质脆嫩,品质佳,适合鲜食。病毒病、疫病和炭疽病的抗性略优于对照福褀新1号;露地地膜覆盖栽培产量3 200 kg/667 m2左右,早春大棚地膜栽培产量4 300 kg/667 m2左右,适于保护地及早春露地栽培。     

辣椒新品种雪峰椒1号的选育

雪峰椒1号是以自交系W648-5为母本、自交系WP为父本配制的一代杂种。该品种早熟，果实长牛角形，黄绿色，果面光滑，有光泽，成熟果鲜红色。果长20cm，果宽3．0cm左右，果肉厚0．30cm，单果重36g左右。果实辣味中等，口感好，较耐贮运。一般春露地早熟栽培每667m^2前期产量1200kg左右，比对照增产16％左右。每667m^2总产量3000kg左右，比对照增产10％-20％。适宜长江流域作春早熟露地栽培，也适宜于南菜北运基地栽培。     

菜豆新品种连农无筋2号的选育

连农无筋2号利用杂交育种,后代经过系统选育而成。它涉及4个亲本：82-3长菜豆、85-1、Cornell49-242、87B。植株蔓生 ,商品荚绿色,扁形,荚长22 cm,荚宽1.5~2.0 cm,荚厚1.2~1.7 cm,荚形指数1.36,单荚质量23.0 g左右。抗锈病,中抗炭疽病 。春、秋露地栽培每667 m²产量分别为3 076、2 243 kg,春季大棚栽培平均每667 m²产量2 500 kg左右,冬季温室栽培平均产量3 200 kg,适合北方地区春秋大棚、春秋露地及冬季温室栽培。     

番茄新品种江蔬1号的选育

江蔬1号番茄是以自交系4170p和C94-3224杂交选育而成的一代杂种.属中早熟,有限生长类型,抗ToMV、枯萎病、叶霉病.果实圆形、红色,果面光滑,果皮厚,耐贮运,商品率高.单果质量210 g,一般春季大棚每667 m2产量4 500 kg左右,高产可达5 500 kg,适于早春大棚栽培及露地地膜覆盖栽培.     

辣椒新品种新椒23 号的选育

新椒23 号是以优良自交系2002053-2-3-1 为母本，以优良自交系2002068-5-2-1 为父本配
制而成的中早熟辣椒一代杂种。植株生长势较强，果实长羊角形，果长24.4 cm，果肩宽2.98 cm，果肉厚
1.92 mm，平均单果质量42.3 g，皮薄，肉质脆，辣味适中，一般每667 m²产量4 000 kg 左右，田间表现
对病毒病、疫霉病的抗性比对照天椒3 号强，适宜温室、大棚早熟栽培及温室秋延后栽培，也适合露地地
膜覆盖早熟栽培。     

黄皮洋葱地膜覆盖高产栽培技术

黄皮洋葱是本地出口创汇蔬菜，通过地膜覆盖等一系列栽培措施的应用，洋葱鳞茎横径平均达10cm以上，单球重2509左右，每667m2产量可达5000kg，产值超过8000元。一、育苗1苗床准备选择肥沃、疏松、易排灌，前茬未种过葱蒜类蔬菜的地块作苗床。结合浅耕细耙，667m2施腐熟优?..     

水果黄瓜新品种京研迷你9 号的选育

京研迷你9 号为保护地水果黄瓜一代杂种。其母本为广州引进的品种09SG-3 经6 代自交选择获得的自交系G3,父本是由欧洲水果黄瓜资源材料65-60 和95-63 杂交后经 6 代自交选择获得的高配合力自交系 M118。京研迷你9 号生长势强,全雌性。瓜长 14～18 cm,瓜皮深绿色,果面光滑,瓜肉淡绿色,口感脆甜,品质好。耐低温弱光,耐热。抗霜霉病、白粉病,中抗黄瓜花叶病毒病（CMV）。越冬温室长季节栽培每667 m² 产量高者可达 12 500 kg,早春温室栽培每667 m² 产量7 900 kg,春大棚栽培每667 m² 产量7 060 kg,秋大棚栽培每667 m² 产量6 500 kg。适合我国北方地区越冬温室、早春温室和春秋大棚种植。     

高山夏季反季节大白菜优质栽培技术

屏南县利用夏季低温凉爽的气候特点栽培高山夏季反季节大白菜,每667 m2产量可达5000～6000kg,平均收益2 000元以上.海拔800～1 200 m地区的适播期是5-6月,采用地膜覆盖栽培,整地时每667 m2施入生石灰50～60 kg,可预防大白菜干烧心.     

早熟丰产辣椒新品种江蔬4号的选育

江蔬4号辣椒是利用来自北方羊角椒8-2-7的优良自交系0736和来自上海 早熟甜椒的优良自交系0517配制的早熟辣椒一代杂种。果实牛角形，纵径14.3cm， 肩横径3.7cm，肉厚0.26cm。抗病毒病和炭疽病。每667m2前期 平均产量1065kg，总产量2700kg，其丰产性、抗病性和 商品品质均优于对照湘研1号。适宜保护地栽培和西南地区露地地膜覆盖栽培。     

日光温室小型西瓜一绳双蔓高产栽培技术

应用一绳双蔓栽培技术,可有效提高种植密度,提高日光温室光热资源利用率。在行距70 cm情况下,一绳双蔓栽培较双绳双蔓栽培株距由50 cm降至30 cm,667 m2种植密度由2 380株提高到3 170株。每667 m2可多定植790株,单瓜质量在1.6 kg左右,667 m2可增加西瓜产量1 200 kg以上,增收6 000元左右。     

Effects of radiation from 188Re on smooth muscle cell proliferation

AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β^-particles emission from ¹⁸⁸Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by ¹⁸⁸Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [³H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of ¹⁸⁸Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from ¹⁸⁸ Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G₀/G₁ arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation.     

Effect of L-Arg on plasma content of endothelin and expression of c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy

AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P＜0.01). The ET content in plasma also decreased significantly by L-Arg(P＜0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration.     

Compositional and Functional Properties of Saskatoon Berry and Blueberry

Abstract

Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared.     

多效唑对猕猴桃离体试管苗生长及内源激素的影响

多效唑（ＰＰ３３３）处理猕猴桃试管苗，降低了其生长强度；植株体内的ＧＡ３、ＩＡＡ和ＺＴ含量下降，ＡＢＡ的含量上升，乙烯释放率增加；并且能降低外源的ＧＡ３和ＩＡＡ促进生长的作用，而外源的ＧＡ３和ＩＡＡ又能不同程度地逆转多效唑的抑制作用，使植株恢复生长。     

Proteomic analysis between pathological scars and normal skin

AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars.     

Cryopreservation of shoot apices of hawthorn in vitro cultures originating from East Asia

The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species.     

Metallothionein inhibits homocysteine-induced proliferation of rat vascular smooth muscle cells

AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [³-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10^-6-10^-4 mmol/L) stimulated [³-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [³-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10^-6-10^-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl₂ for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation.     

Coupling past management practice and historic landscape change on John F. Kennedy Space Center,Florida

Historic landcover dynamics in a scrubby flatwoods (Tel-4) and scrub landscape (Happy Creek) on John F. Kennedy Space Center were measured using aerial images from 1943, 1951, 1958, 1969, 1979, and 1989. Landcover categories were mapped, digitized, geometrically registered, and overlaid in ARC/INFO. Both study sites have been influenced by various land use histories, including periods of range management, fire suppression, and fire management. Several analyses were performed to help understand the effects of past land management on the amount and spatial distribution of landcover within the study sites. A chi-squared analysis showed a significant difference between the frequency of landcover occurrence and management period. Markov chain models were used to project observed changes over a 100-year period; these showed current management practices being effective at Tel-4 (restoring historic landscape structure) and much less effective at Happy Creek. Documenting impacts of past management regimes on landcover has provided important insight into current landscape composition and will provide the basis for improving land management on Kennedy Space Center and elsewhere.     

XBP-01 accelerated apoptosis induced by oxidized low density lipoprotein in vascular smooth muscle cells

AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 h after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 h in vitro. The samples were analyzed by fluorescence microscope, confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis.     

The effect of exposure on landscape scale soil surface temperatures and species distribution models

Species distribution models (SDMs) often use elevation as a surrogate for temperature or utilise elevation sensitive interpolations from weather stations. These methods may be unsuitable at the landscape scale, especially where there are sparse weather stations, dramatic variations in exposure or low elevational ranges. The goal of this study was to determine whether radiation, moisture or a novel estimate of exposure could improve temperature estimates and SDMs for vegetation on the Illawarra Escarpment, near Sydney, Australia. Forty temperature sensors were placed on the soil surface of an approximately 12,000 ha study site between November 2004 and August 2006. Linear regression was used to determine the relationship with environmental factors. Elevation was correlated more with moderate temperatures (winter maximums, summer minimums, spring and autumn averages) than extreme temperatures (summer maximums, winter minimums). The correlation (r ²) between temperature and environmental factors was improved by up to 0.38 by incorporating exposure, moisture and radiation in the regressions. Summer maximums and winter minimums were predominately determined by exposure to the NW and coastal influences respectively, while exposure to the NE and SW was important during other seasons. These directions correspond with the winds that are most influential in the study area. The improved temperature estimates were used in Generalised Additive Models for 37 plant species. The deviance explained by most models was increased relative to elevation, especially for moist rainforest species. It was concluded that improving the accuracy of seasonal temperature estimates could improve our ability to explain the patchy distribution of many species. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.