Yield enhancement and phosphorus economy in lentil (Lens culinaris Medikus) with integrated use of phosphorus,Rhizobium and plant growth promoting rhizobacteria

The study evaluated the effects of phosphorus (0, 20, 30, and 40 kg P₂O₅ ha^?1) and biofertilizers [Rhizobium (Rhizobium leguminosarum bv viciae), plant growth promoting rhizobacteria (PGPR) (Pseudomonas fluorescens), Rhizobium + PGPR, and uninoculated control] in lentil. Application of 40 kg P₂O₅ ha^?1 resulted in the highest number of nodules, nodule dry weight, leghemoglobin content in nodules, chlorophyll content, yield attributes, and grain yield. Coinoculated treatment performed better than uninoculated control, and individual inoculations of Rhizobium and PGPR in terms of all above mentioned parameters. Application of 20 kg P₂O₅ ha^?1 + Rhizobium inoculation gave statistically similar and 20 kg P₂O₅ ha^?1 + Rhizobium + PGPR inoculation gave significantly higher grain yield than that by 40 kg P₂O₅ ha^?1 alone. The use of Rhizobium alone and Rhizobium + PGPR consortium can save not only 20 kg P₂O₅ ha^?1 but also increase the grain yield of lentil.     

Influence of Vesicular Arbuscular Mycorrhizal Fungi and Applied Phosphorus on Root Colonization in Wheat and Plant Nutrient Dynamics in a Phosphorus-Deficient Acid Alfisol of Western Himalayas

Vesicular arbuscular mycorrhizal (VAM) fungi symbiosis confers benefits directly to the host plant's growth and yield through acquisition of phosphorus and other macro- and micronutrients, especially from phosphorus (P)–deficient acidic soils. The inoculation of three VAM cultures [viz., local culture (Glomus mosseae), VAM culture from Indian Agricultural Research Institute (IARI), New Delhi (Glomus mosseae), and a culture from the Centre for Mycorrhizal Research, Energy Research Institute (TERI), New Delhi (Glomus intraradices)] along with P fertilization in wheat in a P-deficient acidic alfisol improved the root colonization by 16–24% while grain and straw yields increased by 12.6–15.7% and 13.4–15.4%, respectively, over the control. Uptake of nitrogen (N), P, potassium (K), iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu) was also improved with VAM inoculation over control, but the magnitude of uptake was significantly greater only in the cases of P, Fe, Zn, and Cu. Inoculation of wheat with three VAM cultures in combination with increasing inorganic P application from 50% to 75% of the recommended P₂O₅ dose to wheat through the targeted yield concept following the soil-test crop response (STCR) precision model resulted in consistent and significant improvement in grain and straw yield, macronutrient (NPK) uptake, and micronutrient (Fe, Mn, Zn, Cu) uptake in wheat though root colonization did not improve at P₂O₅ doses beyond 50% of the recommended dose. The VAM cultures alone or in combination with increasing P levels from 50% to 75% P₂O₅ dose resulted in reduction of diethylenetriaminepentaacetic acid (DTPA)–extractable micronutrient (Fe, Mn, Zn, Cu) contents in P-deficient acidic soil over the control and initial fertility status, although micronutrient contents were relatively greater in VAM-supplied plots alone or in combination with 50% to 75% P₂O₅ dose over sole application of 100% P₂O₅ dose, thereby indicating the positive role of VAM in nutrient mobilization and nutrient dynamics in the soil–plant system. There was significant improvement in available N and P status in soil with VAM inoculation coupled with increasing P levels upto 75% P₂O₅ dose, although the greatest P buildup was obtained with sole application of 100% P₂O₅ dose. The TERI VAM culture (Glomus intraradices) showed its superiority over the other two cultures (Glomus mosseae) in terms of crop yield and nutrient uptake in wheat though the differences were nonsignificant among the VAM cultures alone or at each P level. Overall, it was inferred that use of VA-mycorrhizal fungi is beneficial under low soil P or in low input (nutrient)–intensive agroecosystems.     

EFFICIENCY OF RHIZOBIUM INOCULATION AND P FERTILIZATION IN ENHANCING NODULATION,SEED YIELD,AND PHOSPHORUS USE EFFICIENCY BY FIELD GROWN SOYBEAN UNDER HILLY REGION OF RAWALAKOT AZAD JAMMU AND KASHMIR,PAKISTAN

A field experiment was conducted in continuity of our previous study to assess the effect of Rhizobium inoculation (RI) and phosphorus fertilization (P) on growth, yield, nodulation, and P use efficiency of soybean. Different treatments were i) Rhizobium strains (0, S₃₇₇, S₃₇₉, and the mixture of S₃₇₇+S₃₇₉ i.e. S₀, S₁, S₂, S₃); ii) phosphorus fertilizer (0, 50, 100 kg ha^?1 i.e. P₀, P₁, P₂). Soybean variety NARC-1 was as used as a testing crop. Results indicated that root and shoot growth increased by RI treatments whether used alone or in combination with P. Rhizobium inoculation increased plant height up to 12% while P did not show significant effect. Increases in soot dry weight, root length and root dry weight due to RI and P was 57 and 22%, 42 and 7%, 55 and 25%, respectively, over the control treatment. Number of nodules increased from 73 in the control to a maximum of 151 in S₂ while the number increased from 90 in the control to 147 in P₂. Combine application of strains and P increased nodules number from 65 at S₀P₀ to a maximum of 183 at S₂P₂. Similar response was also observed for nodules mass. Soybean seed yields ranged between 1710 and 2335 kg ha^?1 against 1635 kg ha^?1 in the control indicating a maximum of 43% increase over control. Concentration of N and P in plants and their uptake was significantly increased by RI and P. RI also increased the N and protein content of soybean seed. Apparent recovery efficiency (ARE) of applied P was 10?12% and the agronomic, agrophysiological, recovery, utilization efficiencies, and harvest index of P decreased with increasing P rates. Nodule number significantly correlated with the DM yield (r² = 0.78) and seed yield (r² = 0.63) while P uptake significantly correlated with root length (r² = 0.48) and root mass i.e. dry weight (r² = 0.65). Also a significant correlation existed between N uptake and DM yield (r² = 0.98) and N uptake and seed yield (r² = 0.65), P uptake and DM yield (r² = 0.73), and P uptake and seed yield (r² = 0.83). The results of present study indicated a substantial growth and yield potential of soybean under the hilly region and increase in yield and N₂ fixing potential (nodulation) can be achieved by applying Rhizobium inoculation with P fertilization.     

Fertilizer Economy through Vesicular Arbuscular Mycorrhizae Fungi under Soil-Test Crop Response Targeted Yield Model in Maize–Wheat–Maize Crop Sequence in Himalayan Acid Alfisol

The effect of three vesicular arbuscular mycorrhizae (VAM) cultures with or without inorganic phosphorus (P) was studied in a maize–wheat–maize crop sequence in a P-deficient acidic Alfisol. Application of these three VAM cultures (Glomus mosseae, developed by CSK Himachal Pradesh Agricultural University, Palampur; Glomus intraradices, developed by the Energy and Resources Institute, New Delhi; and Glomus mosseae, developed by the Indian Agricultural Research Institute, New Delhi) alone or with 25% to 75% of recommended P₂O₅ dose based on soil-test crop response (STCR) precision model along with 100% of recommended nitrogen (N) and potassium (K) caused significantly greater grain and straw yield, NPK uptake, and soil nutrient buildup over the control and also increased crop yield and NPK uptake consistently and significantly with increases in applied P from 25% to 75% P₂O₅ dose. Results implied that application of either of the three VAM cultures with 75% P₂O₅ dose can economize the yield-targeted fertilizer P dose to the extent of about one fourth of the P requirement in maize–wheat–maize crop sequence without impairing yield targets and soil fertility in a P-deficient acidic alfisol.     

Under filed conditions,mycorrhizal inoculum effectiveness depends on plant species and phosphorus nutrition

Abstract

The cultivation of horticultural crops, such as green peppers, tomatoes, eggplants and bell peppers is very common in semi-arid Mediterranean climate conditions. Two field experiments were performed to determine the effect of mycorrhizal species, plant species and phosphorus levels on mycorrhizal effectiveness and phosphorus (P) and zinc (Zn) nutrient uptake. In the first experiment, under field conditions, four plants species were inoculated with five arbuscular mycorrhizae (AM) species. In the second field experiment, under the same soil conditions, the same plant species were treated with three levels of phosphorus (P), i.e., control; 50?kg and 100?kg P₂O₅ ha^?1. The most effective mycorrhiza species Claroideoglomus etunicatum selected in the first experiment was used in the second field first experiment. In the first experiment, fruit yield enhancement, yield increase, inoculation effectiveness and nutrient concentration in the plant leaves were analyzed. Under field conditions, plant species growth is strongly dependent on the species of AM fungi. Tomato and green pepper plants were inoculated with Cl. etunicatum, eggplants were inoculated with Funneliformis mosseae and bell peppers were inoculated with Rhizophagus clarus, which are high fruit-yielding plant species. In general, Fu. mosseae and Cl. etunicatum increased the yield of the tomatoes, green peppers and eggplants. It seems mycorrhiza species specific to plant species. In the second experiment, mycorrhizal inoculation with P fertilizer application, in particular a moderate amount of P (50?kg ha^?1 P₂O₅) fertilizer increased the green pepper, bell pepper and tomato fruit yield compared with non-inoculated plants and non-P fertilizer application treatments. Increasing the application of P level reduced the mycorrhizal inoculation effectiveness (MIE). The results indicate that for all four solanaceae family plants 50?kg ha^?1 P₂O₅ is a P level threshold for mycorrhizal development, which enhanced plant growth and addition of fertilizer over 50?kg ha^?1 P₂O₅ reduced MIE. P and Zn uptake were significantly increased with mycorrhizal inoculation. These findings are supported by our hypothesis that mycorrhiza inoculation can reduce mycorrhizal dependent horticultural plants P fertilizer requirement.     

Effect of Vesicular Arbuscular–Mycorrhizal Fungi and Phosphorus Application through Soil-Test Crop Response Precision Model on Crop Productivity,Nutrient Dynamics,and Soil Fertility in Soybean–Wheat–Soybean Crop Sequence in an Acidic Alfisol

A field experiment was conducted in a phosphorus (P)–deficient acidic alfisol of the northwestern Himalayas using three vesicular arbuscular mycorrhizal (VAM) cultures: a local culture developed by CSK Himachal Pradesh Agricultural University, Palampur (Glomus mosseae), VAM culture from Indian Agricultural Research Institute (IARI), New Delhi (Glomus mosseae), and a culture from the Centre for Mycorrhizal Research, The Energy Research Institute (TERI), New Delhi (Glomus intraradices). These were applied alone or in combination with 25 to 75% of recommended P₂O₅ and recommended nitrogen (N) and potassium (K) based on soil-test crop response (STCR) precision model with an absolute control, farmers’ practice, and 100% of recommended P₂O₅ dose based on the STCR model. The results revealed that sole application of either of the three VAM cultures have produced 2.68 to 9.81% and 25.06 to 28.62% greater grain yield than the control in soybean and wheat crops, respectively. Besides greater straw yield, NPK uptake as well as soil nutrient buildup increased. Increase in P fertilization from 25 to 75% of recommended P₂O₅ dose coupled with VAM inoculation with either of the three VAM cultures resulted in consistent and significant improvement in crop productivity (grain and straw yields), NPK uptake, and improved soil nutrient status, though significantly greatest magnitude was obtained with sole application of 100% of the recommended P₂O₅ dose. The targeted grain yields of soybean (25 q ha^?1) and wheat (30 q ha^?1) were achievable with 75% of recommended P₂O₅ dose along with mycorrhizal biofertilizers, thereby indicating that application of efficient VAM fungi with 75% of recommended P₂O₅ dose can economize the STCR precision model fertilizer P dose by about 25% without impairing crop yield targets or soil fertility in a soybean-based cropping system in an acidic alfisol.     

Saving Irrigation Water for Winter Wheat with Phosphorus Application in the North China Plain

Conserving irrigation water resources is a most important measure for sustainable wheat production in the North China Plain. In the present study, the effect of phosphorus (P) application for saving irrigation water was evaluated. The application of fertilizer P increased nitrogen and phosphorus uptake, shoot biomass, head number, seed number and, consequently, grain yield, and increased soil water use and seasonal evapo-transpiration. The lower the volume of irrigation water applied, the more obvious were these effects. When winter wheat was basally fertilized with 88.5 kg P₂O₅/ha and irrigated with 90 mm at the jointing stage, the highest fertilizer P use efficiency, apparent P recovery and net profit (due to irrigation and/or fertilizer P) were obtained. The results suggested that fertilizer P should be used for saving irrigation water resources in the North China Plain.     

EFFECTS OF CONVENTIONAL AND CONTROLLED RELEASE PHOSPHORUS FERTILIZER ON CROP EMERGENCE AND GROWTH RESPONSE UNDER CONTROLLED ENVIRONMENT CONDITIONS

A series of experiments on the effects of form and rate of seed row placed phosphorus (P) fertilizer were carried out under controlled environment conditions using flats of a P-deficient Brown Chernozemic soil from Saskatchewan, Canada. The experiments were conducted in the laboratory and growth chamber using rates of seed row placed granular P fertilizer up to 100 kg P₂O₅ ha^?1. Two forms of monoammonium phosphate fertilizer were compared: 1) conventional MAP granules and 2) controlled release phosphorus (CRP) fertilizer granules (Agrium Inc, Denver, CO, USA.) made with a polymer coating to slow the release of phosphate to soil solution. Six crops were utilized in the study to provide a range of commonly grown cereal, oilseed, pulse and forage crops in Western Canada: wheat (Triticum aestivum), canola (Brassica napus), mustard (Brassica juncea), flax (Linum usitatissimum), yellow pea (Pisum sativum) and alfalfa (Medicago sativum). Parameters measured were percentage of planted seeds that had emerged after two weeks, plant biomass yield, and plant P uptake after four weeks. Most of the crops tested showed no negative impact on emergence with seed row placed conventional P fertilizer at rates up to ～20 to 30 kg P₂O₅ ha^?1. Pea, flax and mustard tended to be most sensitive to injury from high rates of seed placed MAP while wheat was least sensitive. The controlled release phosphorus fertilizer (CRP) product greatly increased the tolerance of crops to high rates of seed row placed P, with rates of 80 kg P₂O₅ ha^?1 placed in the seed row producing no significant injury for most crops. This effect is attributed to the coating reducing the harmful salt effect that occurs when high rates of fertilizer are placed in the seed row in close proximity to the seed. Generally, a rate of 30 kg P₂O₅ ha^?1 was sufficient to produce maximum early season biomass yield and P uptake for both conventional MAP and CRP fertilizers. Large differences in early P availability were not evident between the conventional P and controlled released P fertilizer products.     

Effects of Vesicular Arbuscular Mycorrhizae and Applied Phosphorus through Targeted Yield Precision Model on Root Morphology,Productivity, and Nutrient Dynamics in Soybean in an Acid Alfisol

A field experiment was conducted in a phosphorus (P)–deficient acidic Alfisol in northwestern Himalayas to study the effect of three vesicular arbuscular mycorrhizae (VAM) cultures [VAM_L, local VAM culture (Glomus mosseae) developed by CSK Himachal Pradesh Agricultural University, Palampur, India; VAM_T, VAM culture (Glomus intraradices) developed by Centre for Mycorrhizal Research, The Energy and Resources Institute (TERI), New Delhi, India; and VAM_I, VAM culture (Glomus mosseae) developed by Indian Agricultural Research Institute (IARI), New Delhi, India] on growth, productivity, and nutrient dynamics in rainfed soybean. Plant height, aboveground dry matter, root dry matter, total dry matter, root length, root weight density, Rhizobium root nodule count, root colonization, yield attributes, yield, and nutrient uptake of soybean increased consistently and significantly with increase in inorganic P levels from 25 to 75% of recommended P₂O₅ dose based on targeted yield precision model coupled with various VAM cultures. VAM_T (Glomus intraradices) at each P level showed its superiority over VAM_I and VAM_L. Sole application of any of the three VAM cultures produced similar growth and development parameters as well as grain yield (18.68 to 19.08 q ha^?1) as produced through farmers’ practice (nitrogen at 20 kg ha^?1), indicating that VAM has a vital role in root morphology and nutrient dynamics in a soil–plant system, though significantly greater productivity was obtained with 100% of the recommended P₂O₅ dose based on soil-test crop response (STCR) precision model without VAM inoculation. Targeted grain yield of soybean (25 q ha^?1) was achievable with 75% of the recommended P₂O₅ dose applied with any of the three VAM fungi cultures without impairing soil fertility, thereby indicating that VAM fungi can save about 25% P fertilizer in soybean in P-deficient acidic Alfisols of northwestern Himalayas.     

松嫩平原黑土区玉米生产氮磷配合肥效优化模型的研究

通过对黑土区玉米生产氮磷肥配合多年多眯次的试验研究,建立产量与肥效地优化模型,优化解析出黑土区玉米生产氮磷含最佳用量的Ｎ１７２．９ｋｇ／ｈａ,Ｐ２Ｏ５５７．４ｋｇ／ｈａ,氮磷比为３．０：１,可获得最佳产量７７９１．０ｋｇ／ｈａ;最佳用量范围内,氮,磷用量相同时,平均每公斤氮可增产玉米１４．４ｋｇ,每公斤磷可增产玉米１６．９ｋｇ,磷的肥效比氮好,但氮的增产潜力远比磷大,单施磷可增产玉米７３４．９ｋｇ     

小麦/玉米套作条件下氮、磷配施的肥料效应研究

针对内蒙古河套灌区农业面源污染的现状,本研究以内蒙古河套灌区常规作物小麦和玉米为供试材料,采取"3414"部分实施方案,对氮、磷肥的施用效应及养分交互作用进行了研究,探讨进一步削减当地农业生产过程中的肥料用量的施肥技术。结果表明:小麦/玉米套作条件下,作物产量与氮、磷肥施用量之间满足二次型回归模型,氮肥、磷肥及氮磷交互效应对产量产生显著影响,氮磷交互作用氮磷。在施肥水平较低时,氮、磷肥表现出较好的协同促进作用,在达到产量的极限值后,则表现为无效及拮抗作用;中氮中磷处理能够较好地满足作物生长发育过程中对氮和磷的需求,提高作物对氮、磷的利用率。但随着施肥量的进一步增加,作物植株吸肥量也随之增加,施肥效益降低,肥料利用率持续下降。通过对氮、磷单因素及二因素肥料效应的分析,对施肥水平做进一步优化,得出小麦最佳施氮量为167.67~196.61 kg·hm-2,最佳施磷量为130.43~186.64 kg·hm-2;玉米最佳施氮量为222.10~299.14 kg·hm-2,最佳施磷量为156.14~188.00 kg·hm-2。这将为进一步削减氮、磷配施量,改善当地土壤养分平衡,减轻农业面源污染提供一定的指导作用。     

旱地麦田水肥关系及对产量的影响试验研究

用微区隔离遮雨棚法研究结果分析回归得出，氮肥用量、磷肥用量、供水量与冬小麦产量之间关系。固定肥料用量，在试验供水范围内水分和小麦产量呈近似直线关系；固定供水量，施肥量和小麦产量呈抛物线关系。氮肥用量和供水量之间有明显的正交互作用，磷肥用量与供水量之间交互作用不明显。大田不同降雨年份试验结果表明，施肥增产效果是降雨多＞降雨中＞降雨少的年份，在蓄墒期、生育期降雨量为355、411和523 mm的低、中、高降雨年份，冬小麦达最高产量时的氮、磷肥用量分别为每hm²施N 64.5 kg、P₂O₅52.5 kg；N 27.5 kg、P₂O₅105.0 kg和N 192.0 kg、P₂O₅157.5 kg。     

施磷量对小麦物质生产及吸磷特性的影响

在低磷土壤条件下,以中筋小麦扬麦12号和弱筋小麦扬麦9号为材料,研究了施磷量对小麦物质生产和吸磷特性的影响。结果表明,在施磷量(P2O5)0～180.kg/hm2范围内,植株对磷的吸收量、吸收速率和磷的积累量随施磷量增加而上升;以施磷量108.kg/h2处理的叶面积指数(LAI)、植株茎蘖数、茎蘖成穗率、干物质积累量、花后干物质积累量和子粒产量最高。当施磷量超过108.kg/hm2时,相关物质生产指标则呈下降趋势,说明即使在缺磷土壤上,施磷量有其适宜值。小麦一生对磷的吸收存在两个高峰,出苗至越冬始期为第一个吸收高峰,拔节至孕穗期为第二个吸收高峰。植株磷素积累量的70%～75%是在拔节后吸收,表明拔节期施磷对满足小麦第二个吸磷高峰和磷的最大积累期需磷有重要意义。     

Enhancing micronutrient uptake and yield of wheat through bacterial PGPR consortia

A pot experiment was undertaken under net house conditions, with three rhizobacterial strains AW1 (Bacillus sp.), AW5 (Providencia sp.) and AW7 (Brevundimonas sp.), applied along with 2/3 recommended dose of nitrogen (N) and full dose of phosphorus (P) and potassium (K) fertilizers (N₉₀P₆₀K₆₀). An enhancement of 14–34% in plant biometric parameters and 28–60% in micronutrient content was recorded in treatments receiving the combination of AW1?+?AW5 strains, as compared to full dose of fertilizer application. The treatment involving inoculation with AW5?+?AW7 recorded highest values of % P and N, with a two-fold enhancement in phosphorus and 66.7% increase in N content, over full dose application of P and K fertilizers. A significant correlation was recorded between plant biomass, panicle weight, grain weight, N, P and iron (Fe) with acetylene reduction activity, indicating the significance of N fixation in overall crop productivity. Our study illustrates the multiple benefits of plant growth promoting rhizobacteria (PGPR) inoculation in integrated nutrient management and biofortification strategies for wheat crop.     

Effects of Molybdenum and Phosphorus Fertilizers on Cold Resistance in Winter Wheat

A pot trial with acid yellow-brown soil was conducted to investigate the effects of molybdenum (Mo) and phosphorus (P) fertilizers on cold resistances of winter wheat. Molybdenum was applied at two rates (0 and 0.15 mg Mo kg^?1 soil) and P at four rates [0, 100, 200, and 300 mg phosphorus pentoxide (P₂O₅) kg^?1 soil] in experiment 1. Both Mo and P fertilizers were applied at two rates (0 and 0.15 mg Mo kg^?1 soil; 0, 150 mg P₂O₅ kg^?1 soil) in experiment 2. Seed yield, soluble sugar, water-soluble protein, ascorbic acid (AsA), malondialdehyde (MDA), and abscisic acid (ABA) concentrations were studied. The results indicated that Mo and P fertilizer increased seed yield, soluble sugar, water-soluble protein, and AsA but decreased the MDA. It implied that appropriate Mo applied with P application had beneficial effects on increasing seed yield and enhancing the cold resistance ability through changing biological substances concentration in winter wheat.     

养分专家系统推荐施肥对苏北地区水稻产量和肥料利用率的影响

苏北地区水稻集约化种植体系中存在氮肥施用过量、施肥时期与肥料配比不合理、肥料利用率低等现象,是农民收益增加和农业可持续发展的限制因子。为科学、系统地指导苏北地区稻田施肥,提高水稻养分利用效率并降低环境风险,本研究基于地块土壤性状、产量目标及养分管理措施等信息,运行水稻养分专家系统(Nutrient Expert System, NE)进行施肥推荐,并通过田间试验比较了NE推荐施肥对苏北地区水稻产量、经济效益、养分吸收、肥料利用率的影响。试验共设置5个处理,分别为水稻养分专家系统推荐施肥(NE),基于NE处理的减氮(NE-N)、减磷(NE-P)和减钾(NE-K)处理,以及农民习惯施肥(FP)。结果表明:与FP处理相比, NE处理的氮、磷肥偏生产力分别提高44.77%和6.32%,其中氮肥偏生产力达到显著水平(P0.05);钾肥偏生产力显著降低33.55%(P0.05);氮肥、钾肥回收利用率显著提高4.91%、19.35%,磷肥回收利用率与FP处理基本相同。相比于FP处理,NE处理在减少氮肥投入,保证氮、磷、钾肥平衡施用的条件下,水稻增产2.23%,增收6.24%,但差异不显著;水稻植株籽粒中磷和钾积累量分别增加10.32%和51.63%,其中后者达显著水平(P0.05)。综上所述,水稻养分专家系统在苏北地区依据地块信息和智能化施肥系统,指导优化了氮、磷、钾肥的施用量和施用方法,促进了水稻对氮、磷、钾养分的吸收和利用,提高了肥料利用率,具有较好的增产增收效果,可以在苏北地区推广应用。     

EFFECTS OF SINGLE,DUAL AND TRIPLE INOCULATIONS WITH BACILLUS SUBTILIS,BACILLUS MEGATERIUM AND RHIZOBIUM LEGUMINOSARUM BV. PHASEOLI ON NODULATION,NUTRIENT UPTAKE,YIELD AND YIELD PARAMETERS OF COMMON BEAN (PHASEOLUS VULGARIS L. CV. ‘ELKOCA-05’)

This study was conducted in order to investigate the effects of single, dual, and triple inoculations with Rhizobium, N₂-fixing Bacillus subtilis (OSU-142), and P-solubilizing Bacillus megaterium (M-3) on nodulation, plant growth, nutrient uptake and seed yield of common bean (Phaseolus vulgaris L. cv. ‘Elkoca-05’) in comparison to control and mineral fertilizer application under field conditions in 2006 and 2007 in the cold highland in Erzurum plateau (29° 55′ N and 41° 16′ E with an altitude of 1850 m), Turkey. Bacterial inoculations significantly increased all the parameters investigated compared with the control treatment, equal to or higher than nitrogen (N), phosphorus (P), and NP treatments. The lowest shoot dry weight and chlorophyll content values were recorded in the control treatment and the bacterial inoculations increased shoot dry weight by 19.7–54.3% and chlorophyll content by 34.1–59.3% over control. Nodule dry weight significantly increased in Rhizobium alone treatment. Additionally, nodulation by native soil Rhizobium population was increased in single inoculations of OSU-142 and M-3. Significant increases of the seed yield under different inoculation treatments ranged between by 6.6% (Rhizobium + OSU-142 + M-3) and 12.2% (OSU-142 alone) over the control whereas N, P and NP applications corresponded to increases of 5.6%, 4.0% and 7.4%, respectively. All bacterial inoculations, especially triple inoculation, significantly increased uptake of macronutrients and micronutrients by common bean. In conclusion, seed inoculation with Rhizobium, OSU-142 and M-3, especially OSU-142 alone, may substitute partially costly NP fertilizers in common bean production even in cold highland areas such as in Erzurum.     

施磷水平对晋南旱地冬小麦产量及磷素利用的影响

在自然降水条件下, 通过大田试验研究了施磷量对晋南旱地冬小麦部分抗性指标、产量、磷素利用率以及1 m土壤磷素形态分布特征的影响。结果表明: 施磷可以提高旱地冬小麦抗逆性、穗数, 进而提高产量, 但对穗粒数和千粒重影响不明显。在0~120 kg(P₂O₅)·hm^-2施磷范围内, 小麦生育期旗叶硝酸还原酶(NR)活性、穗数和产量随施磷量增加显著增加, 丙二醛(MDA)和脯氨酸(Pro)含量随施磷量增加显著降低。当施磷量达到180 kg(P₂O₅)·hm^-2时, 旗叶中MDA、Pro含量降低幅度较小, 甚至会升高; NR活性除抽穗期外不再有显著变化, 穗数和产量变化亦不显著。磷素施入土壤后易固定, 导致磷肥利用率偏低, 当季回收率仅为9%~13%, 以施磷60~120 kg(P₂O₅)·hm^-2为最高。1 m土壤各土层Hedley形态磷分布特征表现为: HCl-Pi>Residual-P> HCl-Po>NaOH-Pi>NaHCO₃-Pi>NaOH-Po>H₂O-Pi>NaHCO₃-Po>H₂O-Po, 其中以HCl-P和Residual-P为主, 分 别占全磷的75%和20%左右, H₂O-P、NaHCO₃-P和NaOH-P含量共占全磷的5%左右。施入土壤中的磷素当 季主要被固定在0~20 cm土层, 不同Hedley形态磷增加量总体在0~39.11 mg·kg^-1之间, 且施磷越多, 被固定磷素就越多。综合考虑冬小麦抗逆性、产量及磷素利用率, 当地旱作冬小麦施磷量(P₂O₅))以120 kg·hm^-2左右为宜。     

陕西省关中地区石灰性土壤上影响磷肥肥效因素的探讨 Ⅰ.土壤的某些农化性质对磷肥肥效的影响

我国北方石灰性土壤上磷肥的肥效,很不稳定。近年来各地围绕着这一问题,进行了一些工作。大体看来,着重在肥料配合、施用方法、磷肥品种和作物种类对磷肥肥效影响的报道较多。至于土壤本身对于磷肥肥效的影响,研究的还少。     

秸秆还田替代化肥对黄土旱塬小麦产量及水肥利用的影响

为研究不同秸秆还田量替代部分化肥后对黄土高原冬小麦产量、水肥利用效率和硝态氮积累特征的影响。于2018—2021年在晋南黄土旱塬冬小麦种植区,设置秸秆不还田(S0)、秸秆半量还田(S1/2)、秸秆全量还田(S1)、秸秆2倍量还田(S2)4个还田量处理,研究不同秸秆还田量替代化肥对冬小麦产量形成、水肥利用效率及土壤硝态氮残留的影响。结果表明:在黄土旱塬麦区,秸秆还田替代8.3%~31.9% N和15.7%~63.2% P₂O₅的基础上,冬小麦产量总体随秸秆还田量增加而增加,且在降水丰沛年份,增加秸秆还田量可产生更大的产量效应。3年试验总体表明,S2处理冬小麦平均产量分别较S0、S1/2和S1处理分别高17.5%(P＜0.05),10.4%(P＜0.05),4.3%。连续3年秸秆还田均提高了冬小麦穗数,S2处理冬小麦平均单位面积穗数分别较S0、S1/2和[JP]S1处理高17.1%(P＜0.05),12.3%(P＜0.05),3.6%,不同处理间穗粒数和千粒重差异不显著。播前2 m土壤贮水量总体随着还田量的增加而增加,试验期间S2处理平均贮水量较S0提高8.3%(P＜0.05)。冬小麦生育期耗水量也表现为随着还田量的增加而增加,S2处理平均耗水量较S0处理增加了10.0%(P＜0.05)。不同处理间水分生产效率差异不显著,平均为14.9 kg/(hm²·mm)。在秸秆还田替代部分化肥基础上,旱塬冬小麦肥料利用效率随着秸秆还田量的增加而增加,其中,S2处理平均氮肥偏生产力(PFP_N)、氮肥农学效率(AE_N)、氮肥当季回收率(RE_N)和磷肥偏生产力(PFP_P)较S0处理分别提高66.4%,155.8%,113.5%,105.2%。连续3年秸秆不还田使0—2 m土壤硝态氮残留量较2018年播前提高100.6%,并随水向下淋溶在深层土壤中累积,而秸秆还田处理2 m土层硝态氮累积量均低于2018年播前,S2处理2 m土壤硝态氮残留量最低,为244.8 kg/hm²。综合考虑,晋南黄土旱塬麦区,在秸秆还田替代8.3%~31.9% N和15.7%~63.2% P₂O₅基地上,可增加播前土壤底墒,降低肥料残留,并提高肥料利用效率,进而提高冬小麦产量,其中,以2倍秸秆还田量(平均为7 477 kg/hm²)产生的产量和水肥效应最佳。研究结果可为推进旱作麦区面源污染防控和冬小麦高产高效绿色生产提供理论依据。