INTERRELATION BETWEEN FRUIT MINERAL CONTENT AND PRE-HARVEST CALCIUM TREATMENTS ON ‘GOLDEN SMOOTHEE’ APPLE QUALITY

The effects of different pre-harvest calcium treatments and their interrelation with other nutrients in apples (Malus domestica Borkh, ‘Golden Smoothee’) on quality attributes at harvest and after cold storage and on relation between calcium (Ca) and other nutrients were investigated. Calcium sprays were applied during three-seasons beginning 60 days after full bloom (DAFB), with 6 or 12 applications in the second part of the growing season. It was confirmed that calcium treatments generally resulted in increased flesh firmness at harvest but not sufficient to maintain during storage. Treatments reduced bitter pit and lenticel blotch pit after cold storage, but these disorders were not totally eliminated. The treatments did not influence evolution of titratable acidity and soluble solids. Total fruit calcium increased with number of applications, but this increase was not proportional with number of applications. The beneficial effect of calcium treatments was reflected in the nitrogen/Calcium (N/Ca), potassium/calcium (K/Ca), and (K+Mg)/Ca ratios.     

LEAF MINERAL COMPOSITION OF ‘NOVA’, ‘ROBINSON’ AND ‘FREMONT’ MANDARIN CULTIVARS ON DIFFERENT ROOTSTOCKS

Rootstock selection has a critical importance for mineral nutrition of budded cultivars. This study was conducted at the experimental farm of Mustafa Kemal University, Dörtyol, Turkey. The aim of this study was to investigate the effect of sour orange, Troyer and Carrizo citrange rootstocks on the leaf nutrient contents of ‘Nova’, ‘Robinson’, and ‘Fremont’ mandarin cultivars in the 2004 and 2005 growing seasons. Carrizo citrange was examined for nitrogen (N), potassium (K), magnesium (Mg), sodium (Na), and manganese (Mn); Troyer citrange for iron (Fe); and sour orange for calcium (Ca) and zinc (Zn) were superior on the others. Nitrogen, phosphorus (P) and Mg levels of mandarin leaf budded on sour orange were under or just above the deficiency threshold. Potassium, Mn, and Zn content of ‘Robinson’; P, Ca and Fe content of ‘Fremont’; and Cu content of ‘Nova’ were statistically higher. Therefore, it can be concluded that Carrizo rootstock may be suggested for the region and regions with similar ecological conditions.     

EFFECT OF CITRUS ROOTSTOCKS ON LEAF MINERAL COMPOSITION OF ‘OKITSU’, ‘CLAUSELLINA’, AND ‘SILVERHILL’ MANDARIN CULTIVARS

Citrus performance is strongly related with rootstock. This study was conducted to investigate leaf nutrient contents of ‘Okitsu’, ‘Clausellina’ and ‘Silverhill’ mandarin cultivars budded onto sour orange, ‘Carrizo’ and ‘Troyer’ citrange rootstocks in Dörtyol, Turkey in 2004 and 2005. The maximum nitrogen (N), potassium (K), and copper (Cu) contents were determined for ‘Clausellina’; phosphorus (P) for ‘Okitsu’; and sodium (Na) for ‘Silverhill’. Calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), and zinc (Zn) uptake were similar for the mandarin cultivars. ‘Carrizo’ citrange at N, K, Mg, Mn, and Cu uptake; ‘Troyer’ citrange at N, P, K, and Fe uptake; and common sour orange at Ca, Zn, and Na uptake was superior on the other rootstocks. It was observed that ‘Carrizo’ and ‘Troyer’ citrange rootstocks had advantages over sour orange in nutrient uptake. Thus, growth performance, yield, and quality parameters considered, ‘Carrizo’ and/or ‘Troyer’ citranges could be suggested as rootstocks for the studied mandarin cultivars under similar ecological conditions.     

BIOREGULATORS INFLUENCE CALCIUM CONCENTRATION AND COLD HARDINESS OF YOUNG ‘DELICIOUS’ (MALUS DOMESTICA,BORKH) APPLE TREES

Calcium concentration and cold hardiness in woody shoots of young ‘Delicious’ apple trees increased during the first half of the dormant season by early-fall whole-tree application of bioregulators and/or plant protectant. Compared with untreated controls, the plant protectant, α-[(1,3-dioxolan-2-ylmethoxy) imino] benzene acetonitcile [Hytech Polymer, (formerly ProTec^TM), Agro-K, Corp., Minneapolis, MN, USA] applied in September at 5 or 10% induced more cold hardiness in late October and November, whereas treating at 0.05% with the bioregulator, 2-chloroethyl-methyl-bis(phenylmethoxy)silane [CGA-15281, Novartis International AG, (formerly Ciba-Geigy Limited) Basel, Switzerland], induced greater cold hardiness and higher woody tissue calcium concentration in November and December. Applied in October, CGA-15281 resulted in woody tissue with the highest cold hardiness rating from November through March. From October 28 through 8 December, tissue from control trees had the lowest calcium concentration and frequently the least amount of cold hardiness. In woody stems of young apple trees, cold hardiness was more closely related to calcium concentration than that of the other eleven measured mineral elements; this was especially apparent from November to March (r = ?0.856).     

FOLIAR APPLICATION OF ZINC INFLUENCES THE LEAF MINERAL STATUS,VEGETATIVE AND REPRODUCTIVE GROWTH,YIELD AND FRUIT QUALITY OF ‘KINNOW’ MANDARIN

Deficiency of zinc (Zn) is widespread in many citrus orchards of Pakistan, consequently hampering fruit yield. Effects of foliar applications of zinc sulfate on tree nutrition, growth, productivity and fruit quality were studied on ‘Kinnow’ mandarin. Leaf nitrogen (N), calcium (Ca), and manganese (Mn) were highest for trees sprayed with 0.2% zinc sulfate, while phosphorus (P) and iron (Fe) were highest with 0.6% zinc sulfate and potassium (K) and Zn were highest with 0.8% Zn sulfate applications. Trees sprayed with 0.6% zinc sulfate exhibited highest increase in height, crown width and stem girth, fruit diameter, fruit weight, ascorbic acid contents, and total phenolics compared to all other treatments. Pre-harvest fruit drop was lowest and total number and weight of fruit per tree was highest at harvest with 0.4% zinc sulfate application. In conclusion, foliar application of zinc sulfate up to 0.6% improved tree mineral nutrients, growth, and productivity with better fruit quality in ‘Kinnow’ mandarin.     

EFFECTS OF BAG-CONTROLLED RELEASE FERTILIZER ON NITROGEN UTILIZATION RATE,GROWTH AND FRUITING OF THE ‘FUJI’ APPLE

Bag-controlled release fertilizer is a new type of controlled-release fertilizer designed to meet the needs of the large individual volume characteristic of fruit trees. The effect of bag-controlled release fertilizer on nitrogen (N) utilization, growth-rate, and fruiting was investigated using ‘Fuji’ apple trees (M. domestica Borkh. cv. ‘Fuji’/M.hupehensis Rhed.). The results showed that the available concentration of nutrients in the soil following bag-controlled release fertilizer treatment (BCRT) was more consistent than when application treatments were broadcasted. The BCRT significantly increased N use efficiency, where this was 2.7 or 1.6 times greater than one or four broadcasted treatments, respectively. Trees treated via BCRT were healthier and had more quality spurs due to more constant nutrient concentration in the soil. With the same application amount, the chlorophyll level and Pn content of BCR-treated trees were steadier and higher than those of broadcasted controls; thus, BCRT significantly increased both yield and fruit quality.     

INFLUENCE OF PREHARVEST SPRAYS OF A MIXTURE OF CALCIUM FORMATE,CALCIUM ACETATE,CALCIUM CHLORIDE AND CALCIUM NITRATE ON QUALITY AND ‘JONAGOLD’ APPLE STORABILITY

The aim of the study was to examine effect of preharvest sprays of a mixture of calcium (Ca) formate, Ca-acetate, Ca-chloride (CaCl₂) and Ca-nitrate [Ca(NO₃)₂] on quality and apple storability. The experiment was conducted during 2008–2009 at the Experimental Station of the Research Institute of Horticulture in Skierniewice, Poland, on mature ‘Jonagold’ apple trees/M.26, planted at a moderate density on a coarse-textured soil with low status of organic matter and abundant in exchangeable Ca. Apple trees were sprayed with Ca, 7 times per season, at 2-week intervals, starting 3 weeks after blooming. In the spray treatments a mixture of Ca-formate, Ca-acetate, CaCl₂ and Ca(NO₃)₂ (as Insol Wap fertilizer), CaCl₂ or Ca(NO₃)₂ were used at rates of 7.3 kg Ca, 11.8 kg Ca, and 9.9 kg Ca ha^?1 per season, respectively. The trees sprayed with water were treated as the control. The results showed that Ca sprays caused no leaf and fruit injury. Calcium spray measures did not affect yield, mean fruit weight, and soluble solids concentration (SSC), titratable acidity and starch index of fruit at harvest. However, at harvest apples sprayed with Ca contained more this nutrient and were firmer than those of the control plots. After storage apples sprayed with Ca had more acids, and were firmer and less liable to bitter pit than the control fruit. Calcium sprays did not affect SSC of fruit after storage but reduced postharvest decay of fruit caused by Peziculla spp. and Penicillium expansum fungi. Apples sprayed with Insol Wap material were the least affected by the above pathogens. It is concluded that in apple orchards preharvest sprays of a mixture of Ca-formate, Ca-acetate, CaCl₂ and Ca(NO₃)₂, containing 10% Ca (w/w) with ca. 50% in an organic form, can be the alternative to sprays of CaCl₂ or Ca(NO₃)₂.     

FOLIAR APPLICATION OF BORON INFLUENCES THE LEAF MINERAL STATUS,VEGETATIVE AND REPRODUCTIVE GROWTH,YIELD AND FRUIT QUALITY OF ‘KINNOW’ MANDARIN (CITRUS RETICULATA BLANCO.)

The effects of foliar application of boron (B) in the form of boric acid on vegetative and reproductive growth, yield and fruit quality of ‘Kinnow’ mandarin (Citrus reticulate Blanco.) were investigated. Kinnow mandarin was sprayed with different concentrations of boric acid viz. (0.1%, 0.2%, 0.3% and 0.4%) at fruit set stage. Leaf nitrogen (N), phosphorus (P), potassium (K), B, and zinc (Zn) along with flush length, tree height, tree spread and tree trunk diameter increased, while leaf length and leaf age showed non-significant results after foliar B application. Yield of the ‘Kinnow’ mandarin was significantly affected by foliar application of B and a significant increase in fruit weight at harvest was also observed. Soluble solid concentration (SSC): titratable acidity (TA) ratio, ascorbic acid, total sugars, total phenolic content (TPC) and total antioxidants significantly affected, while pH of juice, SSC, TA, reducing sugars and non-reducing sugars showed non- significant results.     

DO MAIZE AND PEPPER PLANTS DEPEND ON MYCORRHIZAE IN TERMS OF PHOSPHORUS AND ZINC UPTAKE?

Nutrient deficiency, especially zinc (Zn) and phosphorus (P), is a common nutritional problem for the production of some crops in Turkey. This problem results in the application of increasing amounts of several fertilizers. Mycorrhizal inoculation or the indigenous potential of mycorrhizae in the soil is a critical factor in crop production under low supply of Zn and P. The effects of selected mycorrhizal inoculation on growth and Zn and P uptake of maize and green pepper were investigated in Zn- and P-deficient calcareous soils from Central Anatolia. Soils were sterilized by autoclaving and plants were grown for 7 weeks in pots under greenhouse conditions with inoculation of two selected arbuscular mycorrhizal (AM) species (Glommus moseea and G. etunicatum) at three rates of P (0, 25, 125 mg P kg^?1 soil) and two rates of Zn (0 and 5 mg Zn kg^?1soil). Without mycorrhizal inoculation, shoot and root dry matter production were severely affected by P and Zn deficiencies, and supply of adequate amounts of P and Zn significantly enhanced plant growth. When the soil was inoculated with mycorrhizal inoculation, the increasing effects of P and Zn fertilization on plant growth remained less pronounced. In accordance with growth data, mycorrhizae inoculation enhanced P and Zn concentration of plants, especially under low supply of P and Zn. The results obtained indicate that maize and green pepper are highly mycorrizal–dependent (MD) plant species under both low P and Zn supply and mycorrhizae play an essential role in P and Zn nutrition of plants in P and Zn-deficient soils. Although addition of P and Zn increased plant growth and plants are mycorrhizal dependent on P and Zn nutrition however dependence is much more dependent on P nutrition.     

EFFECT OF PREHARVEST SPRAYS OF CALCIUM CHLORIDE AND SUCROSE ON CRACKING AND QUALITY OF ‘BURLAT’ SWEET CHERRY FRUIT

The aim of the study was to examine impact of preharvest sprays of calcium (Ca) and sucrose on splitting and quality of ‘Burlat’ sweet cherry fruit. The study was carried out during 2009–2010 in Poland and Turkey, on mature trees planted at a moderate density on coarse- and medium-textured soils. Sweet cherries were sprayed with calcium chloride (CaCl₂) and/or sucrose at a rate of 5 kg of each material per ha, using ca. 1000 L of water. In Poland, sprays of CaCl₂ and/or sucrose were applied 8–14 h before each rain, starting 21 d before harvest, whereas in Turkey the spray treatments were made schematically: 7, 14 and 21 d before harvest. Sprays of water were treated as the control. The results showed that preharvest sprays of CaCl₂ and/or sucrose did not affect yield, mean fruit weight, and fruit acidity. Sprays of CaCl₂ increased fruit Ca status, and simultaneously reduced fruit cracking in an orchard or laboratory test. In the trial performed in Turkey, part of fruit sprayed with CaCl₂ (<5%) had spray deposit on the peel. Sucrose sprays did not affect fruit cracking. In one year of the study in Turkey, sucrose sprays increased soluble solids concentration of fruit; however, part of fruit treated with sucrose (<2%) had spray deposit on the skin.     

EFFECTS OF FOLIAR AND SOIL CALCIUM APPLICATION ON YIELD AND QUALITY OF TABLE GRAPE CV. ‘THOMPSON SEEDLESS’

Table grape quality and firmness are key parameters for Chile's export, given distances from the main markets. As a consequence, calcium (Ca) fertilization has become a standard practice in the country, although its effects have not been fully evaluated. The objectives in this study were to investigate the effects of calcium fertilization on table grape nutrition and quality. A two-year field trial was conducted in central Chile, using grapevines (Vitis vinifera) cv. ‘Thompson seedless’, grown on an overhead trellis system. Calcium (CaCl₂) application treatments included foliar, soil, foliar plus soil, and a control without Ca fertilization. Calcium and other nutrients concentration were determined afterwards in soil, leaves, and berries. Also, yield and some fruit quality parameters were evaluated. Calcium application method did not have an effect on soil, leaf or fruit Ca content, neither on yield and berry sugar concentration, with levels being similar to the control treatment. Additionally, in treatments where CaCl₂ was applied to soil, chlorides concentration in soil, leaves, and berries were higher. Also, berries were larger, more turgent, with lower dry matter and larger cells. In the second season, in these same treatments, berry firmness at harvest was higher, but this effect disappeared after the storage simulation period. These results question the validity that Ca applications might improve table grape postharvest quality for growing conditions similar to the ones in this experiment. The temporal changes in berry quality parameters were attributed to higher Cl soil content and the associated plant osmoregulatory mechanisms.     

VEGETATIVE GROWTH,YIELD, FRUIT QUALITY AND FRUIT AND LEAF COMPOSITION OF STRAWBERRY CV. ‘PAJARO’ AS INFLUENCED BY SALICYLIC ACID AND NICKEL SPRAYS

Salicylic acid (SA) occurs naturally in plants at low concentrations. Previous studies reported a vast range of responses after SA application on plants. Nickel (Ni) as an essential element for plant growth and development has been proven to have positive impact on overall plant life cycle. To evaluate influence of these two compounds on strawberry plants cv. ‘Pajero’ this study was carried out as a 3 × 4 factorial in a completely randomized design in greenhouse conditions. Treatments included SA at 0, 1, 2, and 3 mM and nickel sulfate (NiSO₄) at 0, 150 and 300 mg L^?1 concentration. It seems that treatments had promoting effects on measured parameters as 2 mM of SA increased root and shoot fresh and dry weight, concentration of anthocyanins and poliphenolics of fruits significantly, 3 mM of this phytohormone caused augmentation of vitamin C content of fruits. Nitrogen and nickel concentration within leaves and fruits were affected after SA treatments. 150 mg L^?1 of nickel solution promoted total yield, root dry weight and nitrogen concentration of fruits. Worth mentioning, beneficial effects of such treatments were higher when applied together. Further research is needed before recommendation on other cultivars and commercial use.     

EFFECTS OF POTASSIUM NITRATE SPRAYING ON FRUIT CHARACTERISTICS OF ‘MALAS YAZDI’ POMEGRANATE

The present research was carried out to investigate the effects of potassium nitrate on pomegranate fruit quality. Treatments were: 1) control, distilled water was used; 2) potassium nitrate 250 mg L-¹ plus Tween-20 and 3) potassium nitrate 500 mg L-¹ plus Tween-20. All treatments were sprayed on fruits with a diameter of 30 mm. Juice, peel, seed characters, and fruit quality components were evaluated when fruits were harvested. Results indicated that 250 mg L-¹ potassium treatment increased juice volume and weight compared with the control and the 500 mg L-¹ K treatments. As the potassium nitrate level increased, aril length and diameter decreased. Although there was not a significant difference among treatments on titrable acidity and pH contents of fruits, the highest total soluble solids and vitamin C were obtained in the 250 mg L-¹ potassium nitrate treatment when compared with the others. These findings show that applied potassium nitrate significantly influences fruit quality of pomegranate when fruit are in the beginning stages of growth and development.     

EFFECT OF AUTUMN CALCIUM SPRAY AT A HIGH RATE ON ‘GRANNY SMITH’ APPLE QUALITY AND STORABILITY

The aim of the study was to examine effect of autumn calcium (Ca) spray at a high rate on apple quality and storability. The investigation was carried out during 2005–2006 in Isparta district, Turkey, under semi-arid conditions, on mature ‘Granny Smith’ apple trees/M.9 EMLA, planted at a spacing of 3.5 × 1.5 m on fine-textured soil rich in Ca. The trees were sprayed with Ca in summer and/or in autumn, using organically complexed Ca to avoid possible leaf and fruit injuries. In autumn (10 days before harvest), the trees were sprayed with Ca at a rate of 8 kg ha^?1. In summer, the another trees were sprayed with Ca six times at a rate of 1.5 kg ha^?1 in each measure; the first spray treatment was performed 6 weeks after full bloom, and the others at 7–9 day intervals. The third part of the trees was sprayed with Ca in summer as well as in autumn, at the same terms and rates as given above. Trees unsprayed with Ca served as the control. It was found that Ca sprays in autumn or in summer plus in autumn damaged leaves; however those treatments did not caused defoliation. Calcium sprays had no effect on apple yield, mean fruit weight, fruit skin russeting, and firmness, soluble solids concentration and titratable acidity of fruit at harvest. Fruit flesh Ca concentrations of the control trees were high. However, the highest fruit Ca concentration was recorded on the trees sprayed with Ca in summer plus in autumn. Calcium sprays in summer or in autumn increased fruit Ca concentrations but their effects were weaker than summer plus autumn Ca applications. After 100 days of refrigerated air storage, soluble solids concentration of fruit did not differ between the studied combinations. Only apples sprayed with Ca in summer plus in autumn were firmer and contained more organic acids than the control fruit. During storage, there were neither pathogenic diseases nor physiological disorders. Based on the obtained results we conclude that Ca sprays in summer plus in autumn at high rate can prolong ‘Granny Smith’ apple storage even thought initial fruit Ca concentration is as high as 502–504 mg Ca kg^?1 DW or 84.1–84.8 mg Ca kg^?1 FW.     

EFFECT OF VARIOUS FOLIAR SPRAYS ON SOME FRUIT QUALITY ATTRIBUTES AND LEAF NUTRITIONAL STATUS OF THE PEACH CULTIVAR ‘ANDROSS’

The effect of various products applied as foliar sprays on some fruit quality attributes and leaf nutritional status of the peach cultivar ‘Andross’ for a three year period is reported. Foliar application of all calcium (Ca) products plus boron (B), did not affect mean fruit weight compared to the control. Application of profical plus calcium phosphite plus power 7-B resulted in an increase of flesh firmness. Low flesh firmness was measured at the potassium nitrate (KNO₃) and Chelan-K treatments. Foliar application of all Ca and K products did not alter significantly total soluble solids, total titratable acidity, and length and width of pits in comparison to the control. The highest amount of compact pits (without visual split) for the three year period was measured after foliar application of silene-K. Profical plus calcium phosphite plus power 7-B, and calcium chloride (CaCl₂) plus power 7-B gave also good results for the three years. Foliar application of agriphos, chelan-K, silene-K, and KNO₃ resulted in an increase of K concentration of leaves compared to the control. Foliar application of CaCl₂, bcazin, power-Ca, profical and calcium phosphite plus profical resulted in an increase of Ca concentration of leaves compared to the control for all the years. The efficacy of the foliar sprays varied from year to year indicating the influence of the climatic conditions on tree nutrition.     

RESPONSE OF ORNAMENTAL SUNFLOWER CULTIVARS ‘SUNBEAM’ AND ‘MOONBRIGHT’ TO IRRIGATION WITH SALINE WASTEWATERS

To explore the possibility that saline wastewaters may be used to grow commercially acceptable floriculture crops, a study was initiated to determine the effects of salinity on two pollen-free cultivars of ornamental sunflower (Helianthus annuus L.). ‘Moonbright’ and ‘Sunbeam’ were grown in greenhouse sand cultures irrigated with waters prepared to simulate wastewaters commonly present in two inland valley regions of California: 1) San Joaquin Valley (SJV) where saline-sodic drainage waters are dominated by sodium (Na⁺) and sulfate (SO ^2? ₄ ) and 2) Coachella Valley (CV) where major ions in tailwaters are Na⁺, chloride (Cl^?), SO ^2? ₄ , magnesium (Mg²⁺), calcium (Ca²⁺), predominating in that order. Ten-day-old seedlings were subjected to five salinity treatments of each water composition, each replicated three times. Electrical conductivities (EC) of the irrigation waters were 2.5, 5, 10, 15, and 20 dS·m^?1. Flowering stems were harvested when about 75% of the ray flowers were nearly horizontal. Stem length and fresh weight, flower and stem diameter were measured. Mineral ion concentrations in upper and lower stems, upper and lower leaves were determined. Sodium was excluded from the young tissues in the upper portions of the shoot and retained in the basal stem tissue. Inasmuch as sunflower is also a strong potassium (K)-accumulator, K⁺/Na⁺ selectivity coefficients were unusually high in the younger shoot organs. Despite a five-fold increase in substrate Ca²⁺ in both solutions, shoot-Ca decreased as salinity increased and this cation was retained in the older leaves. A few of the lower leaves of plants irrigated with ICV waters at EC = 10 dS·m^?1 and higher, exhibited necrotic margins which were undoubtedly caused by high concentrations of Cl^? in the tissues. Flowering stems produced in all treatments met florist quality standards in terms of diameters for stems (0.5 to 1.5 cm) and blooms (8 to 15 cm). Across treatments, stem lengths ranged from 60 to 175 cm. Both ornamental sunflower cultivars proved to be good candidates for production of marketable flowering stems using moderately saline wastewaters.     

EFFECTS OF SINGLE,DUAL AND TRIPLE INOCULATIONS WITH BACILLUS SUBTILIS,BACILLUS MEGATERIUM AND RHIZOBIUM LEGUMINOSARUM BV. PHASEOLI ON NODULATION,NUTRIENT UPTAKE,YIELD AND YIELD PARAMETERS OF COMMON BEAN (PHASEOLUS VULGARIS L. CV. ‘ELKOCA-05’)

This study was conducted in order to investigate the effects of single, dual, and triple inoculations with Rhizobium, N₂-fixing Bacillus subtilis (OSU-142), and P-solubilizing Bacillus megaterium (M-3) on nodulation, plant growth, nutrient uptake and seed yield of common bean (Phaseolus vulgaris L. cv. ‘Elkoca-05’) in comparison to control and mineral fertilizer application under field conditions in 2006 and 2007 in the cold highland in Erzurum plateau (29° 55′ N and 41° 16′ E with an altitude of 1850 m), Turkey. Bacterial inoculations significantly increased all the parameters investigated compared with the control treatment, equal to or higher than nitrogen (N), phosphorus (P), and NP treatments. The lowest shoot dry weight and chlorophyll content values were recorded in the control treatment and the bacterial inoculations increased shoot dry weight by 19.7–54.3% and chlorophyll content by 34.1–59.3% over control. Nodule dry weight significantly increased in Rhizobium alone treatment. Additionally, nodulation by native soil Rhizobium population was increased in single inoculations of OSU-142 and M-3. Significant increases of the seed yield under different inoculation treatments ranged between by 6.6% (Rhizobium + OSU-142 + M-3) and 12.2% (OSU-142 alone) over the control whereas N, P and NP applications corresponded to increases of 5.6%, 4.0% and 7.4%, respectively. All bacterial inoculations, especially triple inoculation, significantly increased uptake of macronutrients and micronutrients by common bean. In conclusion, seed inoculation with Rhizobium, OSU-142 and M-3, especially OSU-142 alone, may substitute partially costly NP fertilizers in common bean production even in cold highland areas such as in Erzurum.     

EFFECT OF PREVIOUS NITROGEN STARVATION ON NO3 − AND NH4 + UPTAKE AND ASSIMILATION ASSOCIATED WITH THE ENDOGENOUS SOLUBLE CARBOHYDRATE UTILIZATION IN MOROCCAN WHEAT SEEDLINGS

Uptake of the two forms of nitrogen NO₃ ^? and NH₄ ⁺ and nitrate reduction have been studied in two varieties of Moroccan wheat seedlings, cvs. Sais and Jouda, cultivated under controlled conditions in a hydroponic medium. The available form of nitrogen in the medium had a direct effect on the rate of nitrogen absorption. A brief nitrogen starvation increased the root length and stimulated accumulation of soluble carbohydrates, especially in the root tissues. A resupply of nitrogen stimulated ammonium and nitrate uptake and assimilation with higher mobilization of the soluble carbohydrates previously accumulated in root seedlings. It is suggested that root nitrogen and carbohydrate status might both regulate nitrate uptake and reduction in Moroccan wheat seedlings.

The seedlings raised with the nitrate supply accumulated nitrate in the vacuole as an osmoticum. Shoots are the preferential site for such accumulation. The accumulation of mineral nitrogen was absent in the seedlings cultivated with ammonium supply but was compensated by accumulation of soluble organic nitrogen.