Bioavailability and Accumulation of Cadmium and Zinc by Sedum plumbizincicola after Liming of an Agricultural Soil Subjected to Acid Mine Drainage

A glasshouse pot experiment was conducted to study the effects of liming on plant growth and zinc (Zn) and cadmium (Cd) accumulation by Sedum plumbizincicola in a heavy-metal-contaminated acidified paddy soil. Lime application significantly increased the soil pH, which reached a maximum of 5.53 after addition of 4.0 g kg^?1 lime to soil, about 1.4 units more than that of the control. Sedum plumbizincicola grew larger after lime application but aboveground biomass did not increase significantly with increasing soil pH. Liming significantly reduced shoot Zn and Cd concentrations and uptake except at the lowest lime application rate (0.5 g kg^?1 lime to soil). This indicates that S. plumbizincicola can grow well in acidic soil at a soil pH of 4.15, and application of lime did not increase plant heavy-metal extraction. Consequently, it is promising to use this plant for Cd and Zn phytoextraction from agricultural soils polluted with acid and metals.     

Tolerance of forage legumes to lime‐stabilized sludge

Abstract

Lime‐stabilized sludge (LSS) from dairy processing waste‐water treatment plants is a desirable product for land application. The material contains lime, which neutralizes soil acidity, and P, which is useful as a plant nutrient. The fineness of the lime and the solubility of P make LSS especially desirable in establishing forage legumes. This greenhouse study had two objectives: to determine a reasonable quantity of LSS for establishing forage legumes such as alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) and to prevent adverse effects on seedlings. Sludge was applied at 0, 2.5, 5.0, 7.5 g kg^‐1 to an acid, low P soil in pots, and alfalfa and red clover seeds were sown. All treatments received 123 μg g^‐1 potassium as KCl. A completely randomized design with four replications was used. Each species was handled as a separate study. Dry matter production was measured at one‐tenth bloom stage. Plant samples were analyzed for P, K, Ca, and Mg content. Soil samples taken at the end of the study were analyzed for pH, organic matter, Bray P, K, Ca, Mg, exchangeable Al, EC, and CEC. The higher quantities of LSS (7.5 g kg^‐1 for alfalfa and 5.0 g kg^‐1 for red clover) had negative effects on seedling germination and establishment. Lime‐stabilized sludge resulted in an increase in total nutrient uptake of Ca, Mg, K, and P up to 5.0 and 2.5 g kg^‐1 in alfalfa and red clover, respectively. In both species significant dry matter yield increases were obtained with LSS up to 5.0 g kg^‐1; however, 7.5 g kg^‐1 caused a reduction in dry matter yield. Based on these results, applications of LSS at 5.0 for alfalfa and 2.5 g kg^‐1 for red clover had positive effects in seedling establishment, nutrient uptake, and dry matter production. Lime‐stabilized sludge application resulted in significant increases in soil pH, available P, Ca, Mg, EC, and CEC; decreases were seen in neutralizable acidity and exchangeable Al levels in soil. This study indicates that LSS is appropriate for the acidic, low P soils of Southern Missouri for alfalfa and red clover establishment and production, if applied in appropriate quantities.     

Influence of Lime and Gypsum on Yield and Yield Components of Soybean and Changes in Soil Chemical Properties

Soybean is one of the most important legume crops in the world. Two greenhouse experiments were conducted to determine the influence of liming and gypsum application on yield and yield components of soybean and changes in soil chemical properties of an Oxisol. Lime rates used were 0, 0.71, 1.42, 2.14, 2.85, and 4.28 g kg^?1 soil. Gypsum rates applied were 0, 0.28, 0.57, 1.14, 1.71, and 2.28 g kg^?1 soil. Lime as well as gypsum significantly increased grain yield in a quadratic fashion. Maximum grain yield was achieved with the application of 1.57 g lime per kg soil, whereas the gypsum requirement for maximum grain yield was 1.43 g per kg of soil. Lime significantly improved soil pH, exchangeable soil calcium (Ca) and magnesium (Mg) contents, base saturation, and effective cation exchange capacity (ECEC). However, lime application significantly decreased total acidity [hydrogen (H) + aluminum (Al)], zinc (Zn), and iron (Fe) contents of the soil. The decrease in these soil properties was associated with increase in soil pH. Gypsum application significantly increased exchangeable soil Ca, base saturation, and ECEC. However, gypsum did not change pH and total acidity (H + Al) significantly. Adequate soil acidity indices established for maximum grain yield with the application of lime were pH 5.5, Ca 1.8 cmol_c kg^?1, Mg 0.66 cmol_c kg^?1, base saturation 53%, Ca saturation 35%, and Mg saturation 13%. Soybean plants tolerated acidity (H + Al) up to 2.26 cmol_c kg^?1 soil. In the case of gypsum, maximum grain yield was obtained at exchangeable Ca content of 2.12 cmol_c kg^?1, base saturation of 56%, and Ca saturation of 41%.     

Liming Effects on Extractable Boron in Six Acidic Soils

A laboratory incubation study was conducted to study the influence of liming on extractable boron (B) in acidic soil. Six soils, three each from the Inceptisols and Alfisols, were incubated for 30 days with combinations of lime and B. The soils were acutely deficient in plant-available B (0.09–0.21 mg kg^?1). Only <50 percent of applied B was recovered from the soils in available form. Such recovery was lower in Alfisols than that in Inceptisols due to adsorption of a greater amount of added B with iron (Fe^?) and aluminium (Al^?) oxides in the former soil group. Required dose of lime showed an increase in availability of native soil B, particularly in Inceptisols (26 percent), and a net decrease in recovery of added B (32.5 percent) as compared to no lime control (41.6 percent). The results thus suggest that liming to acidic soils increases extractable B.     

Effect of molybdenum,phosphorus, and lime application to acid soils on dry matter yield and molybdenum nutrition of lentil

Deficiency of molybdenum (Mo) in acid soils causes poor growth of pulses. An experiment was, therefore, conducted in greenhouse to study the effect of Mo, phosphorus (P), and lime application on the dry matter yield and plant Mo concentration of lentil (Lens esculenta L.) in two Mo‐deficient acid alluvial soils. The experiment was conducted using a factorial design with three levels of lime (no lime, half, and full lime requirement), three levels of P (0, 25, and 50 mg kg^‐1), and two levels of Mo (0 and 1.0 mg kg^‐1). Plants were grown for 60 days and at harvest their dry matter yield and Mo concentration were recorded. The three treatments significantly increased dry matter yield, Mo concentration and Mo uptake, the increase being most pronounced with Mo application followed by lime and P. Increases due to applied Mo were greater in presence than in absence of added P; while the reverse was true with the liming treatments. Liming and P application at their lower levels also interacted positively for better Mo nutrition of plants. Results thus indicated that the severity of Mo deficiency in the lentil plants may be reduced by lime and P application in Mo‐deficient acid alluvial soils.     

Internal Boron Requirement of Young Sunflower Plants: Proposed Diagnostic Criteria

Abstract

In a greenhouse study, a significant increase in sunflower (Helianthus annuus L., cv. Hysun 33) dry matter yield was observed with boron (B) application to a B-deficient (hot water-extractable, 0.23 mg B kg^?1) calcareous soil of Missa series (Typic Ustochrept). Six rates of B, ranging from 0 to 8 mg B kg^?1 soil, were applied as H₃BO₃ along with adequate basal fertilization of nitrogen (N), phosphorus (P), potassium (K), and zinc (Zn). Four plants of sunflower were grown in each pot; two were harvested after 4 weeks of germination and the other two after 8 weeks. Maximum crop biomass was produced with 1.0 mg B kg ^?1, and application of ≥2.0 mg B kg^?1 proved toxic, resulting in drastic yield suppressions. Critical B concentration range for deficiency diagnosis in 4‐week‐old sunflower whole shoots appears to be 46–63 mg B kg^?1. However, critical concentration in 8‐week‐old plants was much less (i.e., 36 mg B kg^?l), presumably due to a dilution effect. As plant's internal B requirement can vary, in fact manifold, depending on the species, plant part, and plant age, only a relevant criterion can help in diagnosing the deficiency effectively.     

Effect of Lime and Flooding on Phosphorus Availability and Rice Growth on Two Acidic Lowland Soils

Abstract

Loss of soil‐water saturation may impair growth of rainfed lowland rice by restricting nutrient uptake, including the uptake of added phosphorus (P). For acidic soils, reappearance of soluble aluminum (Al) following loss of soil‐water saturation may also restrict P uptake. The aim of this study was to determine whether liming, flooding, and P additions could ameliorate the effects of loss of soil‐water saturation on P uptake and growth of rice. In the first pot experiment, two acid lowland soils from Cambodia [Kandic Plinthaqult (black clay soil) and Plinthustalf (sandy soil)] were treated with P (45 mg P kg^?1 soil) either before or after flooding for 4 weeks to investigate the effect of flooding on effectiveness of P fertilizer for rice growth. After 4 weeks, soils were air dried and crushed and then wet to field capacity and upland rice was grown in them for an additional 6 weeks. Addition of P fertilizer before rather than after flooding depressed the growth of the subsequently planted upland rice. During flooding, there was an increase in both acetate‐extractable Fe and the phosphate sorption capacity of soils, and a close relationship between them (r²=0.96–0.98). When P was added before flooding, Olsen and Bray 1‐extractable P, shoot dry matter, and shoot P concentrations were depressed, indicating that flooding decreased availability of fertilizer P. A second pot experiment was conducted with three levels of lime as CaCO₃ [to establish pH (CaCl₂) in the oxidized soils at 4, 5, and 6] and four levels of P (0, 13, 26, and 52 mg P kg^?1 soil) added to the same two acid lowland rice soils under flooded and nonflooded conditions. Under continuously flooded conditions, pH increased to over 5.6 regardless of lime treatment, and there was no response of rice dry matter to liming after 6 weeks' growth, but the addition of P increased rice dry matter substantially in both soils. In nonflooded soils, when P was not applied, shoot dry matter was depressed by up to one‐half of that in plants grown under continuously flooded conditions. Under the nonflooded conditions, rice dry matter and leaf P increased with the addition of P, but less so than in flooded soils. Leaf P concentrations and shoot dry matter responded strongly to the addition of lime. The increase in shoot dry matter of rice with lime and P application in nonflooded soil was associated with a significant decline in soluble Al in the soil and an increase in plant P uptake. The current experiments show that the loss of soil‐water saturation may be associated with the inhibition of P absorption by excess soluble Al. By contrast, flooding decreased exchangeable Al to levels below the threshold for toxicity in rice. In addition, the decreased P availability with loss of soil‐water saturation may have been associated with a greater phosphate sorption capacity of the soils during flooding and after reoxidation due to occlusion of P within ferric oxyhydroxides formed.     

Effect of sewage sludge and its biochar on chemical properties of two calcareous soils and maize shoot yield

Little information is available regarding the effect of sewage sludge biochar on soil properties and crop yield. Thus, our objective was to evaluate the effect of sewage sludge (S) and its biochar (B) on maize shoot yield, nutrients and heavy metals uptake in two calcareous soils. The amendments were applied at the rates of 0, 10, 20 and 40 Mg ha^?1. Moreover, NK treatment was included to compare the effects of S and B with conventional fertilization. At harvest time, plant shoots and soil samples were collected for yield, nutrients uptake and chemical analyses. The highest shoot dry matter was obtained in the S treatment. The B application in the clay loam and loam soils resulted in 5.2% increment and 17.7% decrement of shoot dry matter relative to the control, respectively. Shoot dry matter in the NK treatment was significantly higher than in the control. B application decreased Fe, Zn, Mn, Cu and Pb uptake by maize shoot. DTPA-extractable Pb in B-amended soils was lower than in control, while an inverse trend was obtained for available Fe, Zn, Mn and Cu. Biochar application at the rate of 7.3 Mg ha^?1 might be suggested for maize cultivation in clay loam soils.     

Influence of Lime and Gypsum on Growth and Yield of Upland Rice and Changes in Soil Chemical Properties

Upland rice is an important crop in the cropping systems of South America, including Brazil. Two greenhouse experiments were conducted to determine influence of lime and gypsum on yield and yield components of upland rice and changes in the chemical properties of an Oxisol. The lime rates used were 0, 0.71, 1.42, 2.14, 2.85, and 4.28 g kg^?1 soil. The gypsum rates were 0, 0.28, 0.57, 1.14, 1.71, and 2.28 g kg^?1. Lime as well as gypsum significantly increased plant height, straw and grain yield, and panicle density in a quadratic fashion. Adequate lime and gypsum rates for maximum grain yield were 1.11 g kg^?1 and 1.13 g kg^?1, respectively. Plant height, straw yield, and panicle density were positively related to grain yield. Lime as well as gypsum application significantly changed extractable calcium (Ca), magnesium (Mg), hydrogen (H)+aluminum (Al), base saturation, and effective cation exchange capacity. In addition, liming also significantly increased pH, extractable phosphorus (P) and potassium (K), calcium saturation, magnesium saturation, and potassium saturation. Optimum acidity indices for the grain yield of upland rice were pH 6.0, Ca 1.7 cmol_c kg^?1, base saturation 60%, and calcium saturation 47%. In addition, upland rice can tolerate 42% of acidity saturation.     

Effects of limestone and phosphorus on nutrient availability and coastal bermudagrass yield on an ultisol

Abstract

This field study was conducted to evaluate nutrient availability and Coastal bermudagrass [Cynodon dactylon (L.) Pers.] yield response to factorial combinations of applied limestone and P in a strongly acid (pH 4.7), infertile soil. Limestone was applied at rates of 0, 672, and 3808 kg ha^‐1 to a Lilbert loamy fine sand (loamy, siliceous, thermic, arenic Plinthic Paleudult). Phosphorus was applied at rates of 0, 30, 60, 90, 120, 240, and 480 kg P ha^‐1. Soil pH in the surface 15 cm initially increased to 6.2 in response to the high limestone rate, but subsequently declined due to N fertilization. Lime increased soil test P, Ca, and Mg and decreased K and Al. The efficiency of increasing soil test P with fertilizer P was low, but improved as a consequence of liming. Coastal bermudagrass yield increased by as much as 37 percent from P application. Maximum yield coincided with 10 to 15 mg kg^‐1 or greater soil test P and tissue P concentrations that ranged from 1.6 to 2.2 g kg^‐1. Lime Increased tissue Ca and Mg, but had no effect on plant P concentrations. Yield was unaffected by lime despite its positive effect on soil P and an apparent K‐Mg antagonism. Plant nutrients obtained from deep rooting of the bermudagrass into an argiilic horizon may have precluded any positive effect of lime on Coastal bermudagrass yield.     

Phosphorus availability to corn from wood ash-amended soils

Wood ash is a residual material produced during biomass burning. In the northeastern United States up to 80 % of the ash is spread on agricultural lands as a liming amendment with the remainder being disposed of in landfills. As well as raising soil pH, wood ash also adds plant nutrients to soil. This study is an examination of the plant availability of the P in 8 different soils amended with one wood ash. Plant availability was assessed by measuring the biomass and P concentration of corn (Zea mays) L.) plants grown in the greenhouse for 28 d in soil amended with either CaCO₃ (control), wood ash to supply 200 mg kg^?1 total P, or monocalcium phosphate (MCP) to supply 200 mg kg^?1 total P and CaCO₃. Both corn growth and P uptake were highest in the MCP treatments, intermediate in the wood ash treatments, and lowest in the controls for all soil types. The soil property which seemed to have the greatest influence on P availability was pH buffer capacity. The soils with the greatest capacity to buffer OH additions also tended to exhibit the greatest absolute P uptake from wood ash-amended soils and the greatest P uptake relative to that from MCP-amended soils. The ability of soil test extractants to predict uptake of P in the three soil treatments was examined. A buffered ammonium acetate extradant overestimated P availability in the ash-amended soils relative to the MCP-amended soils. An unbuffered, acid, fluoride-containing extract provided a measure of P levels that was consistent with P uptake from all soil treatments. In this study the predictive relationship was as follows: P uptake = 0.017× (Bray P, mg kg^?1) + 1.19; r = 0.81.     

Determining critical limit of boron in soil for wheat (Triticum aestivum L.)

Abstract

Critical values of boron (B) for wheat nutrition in soil and plant were determined through a pot experiment with twenty-one surface soils of Alluvial flood plain and Red-latertic belt comprising three major soil orders (Entisols, Alfisols, Inceptisols) with four levels of boron. Application of boron significantly increased the dry matter yield as well as uptake of B by plants. Critical concentration of hot calcium chloride (CaCl₂) extractable B in soil for wheat was found to be 0.53?mg?kg^?1. The critical plant B concentration varied with growth stages and values were 7.4?mg?kg^?1 at panicle initiation and 4.18?mg?kg^?1 at maturity, respectively. The findings of this investigation also recommend the application of 2?kg?B^?1?ha^?1 for ensuring B sufficiency to wheat in Indo-gangetic alluvial and Red-Lateritic soils.     

Chemical fractions and response of cauliflower (Brassica oleracea var. botrytis) to soil applied boron

The present study was conducted with an objective to estimate the distribution of boron (B) application in various soil fractions and their plant response for assessing the availability in the soil. Two soils (alluvial and red soil) and five levels of B (0, 0.5, 1, 2 and 3?mg B kg^?1 soil) were applied in the pot experiment, and pots were sown with cauliflower (Sabour Agrim) arranged in a completely randomized block design (CRD) with three replications. Result showed that the curd yield of cauliflower increased significantly upto 2?mg B kg^?1 soil irrespective of soils. The percent yield increase was 14.78 and 15.01 in alluvial and red soil over the control, respectively. The initial total B content was 35.88 (alluvial soil) and 15.51 (red soil) mg·kg^?1. The mean content of Fraction I, II, III, IV and V in alluvial soil was 1.11, 1.54, 0.65, 1.49, and 95.18% and in red soil was 2.68, 4.47, 6.62, 2.50, and 83.59% of the total soil B, respectively. For changes in amount of B fractions due to B applications there was significant effect on all the fractions except Fraction II. The increase in apparent B uptake was 0.43?mg B kg^?1 in alluvial soil and 0.25?mg B kg^?1 in red soil over the control (0?mg B kg^?1 soil). Regression equation of yield and B fractions showed the relationship between first four fractions to the yield. Residual fraction was found to be collinear during calculation. Overall the study predicted the bioavailability and dynamics of B in the two distinct soils.     

Silicon availability of the nursery bed soils and effects of silicon fertilizer applied on the growth and silicon uptake of rice Oryza sativa L. seedlings

(pp. 41–46)

Silicon availability in 36 commercial nursery bed soils was evaluated by four methods the phosphate buffer (pH 6.2, 40 mmol L^?1), incubation, supernatant and acetate buffer (pH 4.0, 1 mol L^?1) Methods. The influence of silicon availability in the nursery bed soils on the silicon uptake of rice Oryza sativa L. cv. Hitomebore seedlings and the effect of silicon fertilizer application were examined in a glass house in 2002.

The results revealed that the best correlation between silicon content in rice seedlings and available silicon in soils was obtained with the phosphate buffer-solution method (r = 0.86). More precise evaluation of available silicon was achieved by grouping soils based on these phosphate absorption coefficients (PAC). The correlation coefficients between silicon content in rice seedlings and available silicon in soils were 0.92 and 0.72 for volcanic soils (PAC > 1500) and non volcanic soils (PAC < 1500), respectively.

We concluded that the phosphate buffer method is the most easily adjusted method for estimation of silicon availability in nursery bed soils, and silicon fertilizers should be applied when silicon availability in non-volcanic nursery bed soils goes below 200 mg kg^?1, whereas the level is less than 350 mg kg^?1 in volcanic soils.     

Phosphorus Uptake by Young Citrus Trees in Low-P Soil Depends on Rootstock Varieties and Nutrient Management

Because low-phosphorus (P) availability limits citrus growth, rootstocks with a relatively high capacity for P uptake are desirable. An experiment was conducted with trees on Cleopatra mandarin (CM) and Rangpur lime (RL). Treatments consisted of P rates (20, 40, and 80 mg kg^?1 of soil) applied in soil layers of 0–0.30 m and/or 0.31–0.60 m, besides an unfertilized control. The P fertilization increased root and shoot growth, and P nutrition was improved as indicated by greater leaf P concentration, P uptake, and P root uptake efficiency (PUE). The P applied in both soil layers improved shoot growth, P uptake, and PUE. Trees on RL took up 23–126% more P and had root systems with greater growth and PUE compared to those on CM. Thus, P uptake by citrus trees in low-P soils can be improved by augmenting the depth of fertilizer application and the use of more adapted rootstocks.     

Assessment of critical limit of boron for cowpea in piedmont soils of Arunachal Pradesh

A study was conducted with 24 piedmont soils of Arunachal Pradesh (India) to estimate the critical limit of available boron (B) in soil and cowpea plant for predicting the response of cowpea (Vigna unguiculata L. Walp.) to B application. The critical concentration of available B in these soils was established by graphical procedure at 0.48 mg kg^?1 for soil and at 24.5 mg kg^?1 in the plant tissues. Soil containing available B below the critical limit responded appreciably to B fertilization. A negative response to B application was also observed in soils at higher level of available B. The average dry matter yield increased with the increasing level of B application up to 1.5 mg kg^?1. The yield response to B application in cowpea on B deficient soils was 34.5%. Based on the critical value of 0.48 mg kg^?1, 10 soils were rated to be adequate and 20.0% soils belonging to this category responding to B application, whereas 85.7% soils below the critical value showed a positive response to B application. The hot water soluble B was significantly and positively correlated with organic carbon, cation exchange capacity and clay contents and negatively correlated with pH, silt and sand contents of soils.     

Effect of Sheep Manure and Its Produced Vermicompost and Biochar on the Properties of a Calcareous Soil after Barley Harvest

ABSTRACT

Conversion of manures to vermicompost and biochar may alleviate some negative effects of manure application to soil but the efficiency of the produced vermicompost and biochar as compared to their feedstocks is not well-known. In the current investigation, we compared the effects of sheep manure and its derived vermicompost and biochar (pyrolyzed at 400°C for 4 h) on the properties of a calcareous soil that planted with five cultivars of barley (Behrokh, Khatam, Reyhaneh03, Fajr 30 and Nimrooz) for 60 days. Different soil properties and availability of nutrients and barley yield were determined after plant harvest. The biochar significantly increased barley yield rather than control (4.20 vs. 3.57 g pot^?1), but sheep manure and vermicompost had no effect on it (3.51 and 3.37 g pot^?1, respectively). Fajr 30 and Nimrooz (3.52 and 3.42 g pot^?1, respectively) had significantly lower yield than other cultivars. Biochar increased soil pH up to 8.2. Soil salinity was increased by application of all organic materials (increase to 16–36%). Cation exchange capacity (CEC) and organic matter content of soil were also increased by all organic materials application (0.4–0.9 cmol kg^?1 and 0.33–0.50%, respectively). All organic materials increased total nitrogen (N), but this increase was the highest with sheep manure application (53%). The availability of phosphorus (P) and potassium (K) was increased significantly by application of all organic materials, and this increase was the highest with biochar application (19 and 309 mg kg^?1, respectively). Biochar application had no effect on the availability of micronutrients, but application of sheep manure and vermicompost increased the availability of iron (Fe) (0.62 and 0.48 mg kg^?1, respectively) and zinc (Zn) (0.18 and 0.37 mg kg^?1, respectively). Generally, organic materials may change the status of soil nutrients via change in soil pH, organic matter content, release of nutrients, increase in soil CEC and formation of soluble complex with nutrients.     

Boron fertilization of Mediterranean aridisols improves lucerne (Medicago sativa L.) yields and quality

Abstract

Lucerne (Medicago sativa L.) is grown as a forage crop on many livestock farms. In calcareous soils in eastern Turkey, lucerne production requires boron (B) addition as the soils are naturally B deficient. Field experiments with four B-application rates (0, 1, 3, and 9 kg ha^?1 B) were conducted in 2005 and 2006 to determine the optimum economic B rate (OEBR), critical soil test and tissue B values for dry matter (DM) production for lucerne grown on B-deficient calcareous aridisols in eastern Turkey. Boron application increased yield at each site in both years of production. The OEBR and critical soil and tissue B content were not impacted by location. Averaged over the two years and three locations, the OEBR was 6.8 kg B ha^?1 with an average DM yield of 12.0 Mg ha^?1. The average soil B content at the OEBR was 0.89 mg kg^?1 while leaf and shoot tissue B content amounted to 51.8 and 35.5 mg kg^?1, respectively. Boron application decreased tissue calcium (Ca), zinc (Zn), and copper (Cu), and increased tissue nitrogen (N), phosphorus (P), potassium (K), magnesium (Mg), iron (Fe), and manganese (Mn). Tissue and soil B increased without impacting yield at B levels up to 9 kg ha^?1. We conclude that 7 kg ha^?1 B is sufficient to elevate soil test B levels from 0.11 to 0.89 mg kg^?1 and overcome B deficiency at each of the sites in the study. Similar studies with different soils and initial soil test B levels are needed to conclude if these critical soil and tissue values can be applied across the region.     

Liming and Cultivars Affect Root Growth,Nodulation, Leaf to Stem Ratio,Herbage Yield,and Elemental Composition of Alfalfa on an Acid Soil

Abstract

Soil acidity is one of the limiting factors affecting the production and sustainability of pastures and crops in many parts of the world. An on‐farm experiment was conducted in Australia to investigate the cultivar variation in alfalfa (lucerne) (Medicago sativa L.) with respect to soil acidity and response to applied lime. The experimental site was a brown sandy clay loam with a soil pH of 4.8 (1:5 calcium chloride). Ten cultivars (Hunter River, Hunterfield, Sceptre, Aurora, Genesis, Aquarius, Venus, PL90, PL55, and breeding line Y8804) were tested at two levels of lime (0 and 2 t ha^?1). Lime application significantly increased the root growth, nodulation, leaf retention, leaf to stem ratio, herbage yield, and crude protein content of alfalfa. Liming had a significant effect on elemental composition of alfalfa shoots. Aluminum (Al) concentration was reduced from 93 mg kg^?1 DM in nil lime treatment to 45 mg kg^?1 DM in +lime treatment. Similarly, manganese (Mn) and iron (Fe) shoot concentrations were reduced from 74 mg kg^?1 DM and 92 mg kg^?1 DM to 59 mg kg^?1 DM and 76 mg kg^?1 DM, respectively. Liming significantly improved the calcium (Ca) concentration of shoots, while there was a little effect on phosphorus (P) and zinc (Zn) concentrations of alfalfa shoots. Cultivars had differential response to lime application. Response to lime application was greater in Y8804 and Aurora alfalfa where yield increased by 32% and 31%, while yield increase was 11–22% in other cultivars. Cultivars also differed significantly in root growth, nodulation, leaf drop, leaf to stem ratio, crude protein content, and elemental composition of shoots. Cultivars with better performance in no liming treatment had comparatively lower shoot Al, Mn, and Fe concentrations compared with other cultivars.     

Effect of phosphogypsum application and bacteria co-inoculation on biochemical properties and nutrient availability to maize plants in a saline soil

Phosphogypsum (PG), which contains Ca, P and S and has an acidic effect, may be applied to manage soil constraints such as alkalinity and salinity. For increasing nutrients bioavailability, biofertilizers are commonly applied. Therefore, the aim of this study was to assess PG effect either alone or in combination with the mixed co-inoculation of plant growth promoting rhizobacteria on a saline soil. In a greenhouse pot experiment with maize (Zea mays L.), the inoculated and non-inoculated saline soils were treated with PG at 10 g kg^?1 (PG10), 30 g kg^?1 (PG30), and 50 g kg^?1 (PG50). The soil pH, electrical conductivity (EC_e), and macro-(NPK) and micronutrients (Fe, Mn, Zn, and Cu) availability to mays were examined. Applying PG reduced soil pH and co-inoculation induced significant decreases in soil EC_e. Applying PG increased significantly soil available P. Applying PG combined with co-inoculation effectively increased the soil available K. The soil available micronutrients decreased significantly with PG. However, the inoculated maize treated with PG showed significant higher dry weight (82.1–127.4%) and nutrients uptake than the control. It could be concluded that PG along with co-inoculation may be an important approach for alleviating negative effects of salinity on plant growth.