Phosphate sorption isotherms for fertilizer p needs of sweet corn (Zea mays) grown on a high phosphorus fixing soil

Abstract

Phosphorus sorption isotherms were used to estimate phosphorus fertilizer rates on a phosphorus fixing Alfic Fragiorthod soil from north Idaho. Five rates ranging from 0–584 kg/ha corresponded to sorption equilibrium solution levels from 0.06 to 0.20 ppm P. Yield of sweet corn (variety Spancross) grown two successive years was related to the sorption equilibrium solution P level. Whole plant weight, weight of ears, number of ears, and ear size approached maximum when the sorption equilibrium solution P level was near 0.13 ppm. The P content of leaves at early tassel stage increased with increasing sorption equilibrium solution P in a nearly straight line relationship. The leaves contained 0.27% P when the sorption equilibrium solution P was 0.13 ppm, the level producing maximum yield. The 0.20 ppm P resulted in 0.41% P in corn leaves.     

Decomposition and nitrogen mineralization from legume and non-legume crop residues in a subarctic agricultural soil

An understanding of the C and N dynamics of crop residues is important for efficient nutrient management. The present experiment was conducted to determine the rate of mass and N loss from alfalfa, faba bean, barley, and rape crop residues in a subarctic agricultural soil. Mass, C, and N losses were measured from residues contained in mesh bags and placed on the soil surface or buried 15 cm below the surface. The mass loss from October, 1988, to May, 1989, was the same for surface and buried alfalfa, barley, and rape residues, averaging 40, 20, and 61%, respectively, while surface and buried faba bean residue sustained 30 and 40% mass loss, respectively. The mass loss of the buried residues continued over the summer but not of those placed on the soil surface, resulting in an average 23% greater mass loss of the buried materials after 1 year. The N loss from October to May was similar from the surface and from the buried placements for the alfalfa, faba bean, and rape residues, averaging 11.3, 10.3 and 38.4 g N kg^-1 residue, respectively, while the surface and buried barley lost 2.9 and 4.2 g N kg^-1, respectively. The C:N ratio of all of the residues increased during the winter. These data indicate that the rate of decomposition and N mineralization from crop residues in subarctic environments can equal that measured in temperate climates. Furthermore, the concurrent loss of mass and N combined with an increase in the C:N ratio of the residues suggests that physical rather than biological processes were functioning during the winter. Most of the mass and N loss from these residues occurred during the winter, out of phase with crop demand, thereby creating the potential for N loss from the system and inefficient use of crop residue N.     

Estimation of maximum permissible levels of cadmium in a light soil by using cereal plants

A pot experiment was conducted in a greenhouse to establish the threshold level of Cd in a light soil planted with cereals. During three consecutive years barley, wheat, rye, and corn were grown in the soil treated with 2.5; 5; 25 g g^–1 of Cd applied in sulphate form. The Cd concentration of 5 g g^–1 (plus native Cd content of the soil) was considered as the maximum permissible Cd level in a soil for the soil-plant systems studied. Besides the estimation of total Cd concentrations, several forms of Cd in soils (exchangeable, carbonate, Fe-Mn oxides, organic, and residual) were determined by mean of sequential extraction to define the phytoavailable form of Cd in the soil. The Cd concentration in studied plants increased with the doses of this metal in soil but to different extents for various plant species and various plant parts. The highest Cd enrichment ratios (ER) were found for wheat and corn leaves (2 to 3), whereas, the lowest ERs were detected for corn and rye grains (0.05 to 0.2) grown at the treated soils. All samples of the control plants had ER below 1. A close relationship was found between Cd content of plants and the exchangeable form of Cd in soil, which indicates that this form of the element is readily available for plants. The proposed maximum permissible level of Cd (5 g g^–1 of added metal plus native Cd content of soils) in light soils proved to be too high for cereal plants. The threshold concentration for light soil should not exceed 3 g g^–1.     

Utilization by wheat crops of nitrogen from legume residues decomposing in soils in the field

Ground ¹⁵N-labelled legume material (Medicago littoralis) was mixed with topsoils in confined microplots in the field, and allowed to decompose for 7 and 5 months in successive years (1979, 1980) before sowing wheat. The soil cropped in 1979 (and containing ¹⁵N-labelled wheat roots and legume residues) was cropped again in 1980.The results support evidence that ungrazed legume residues, incorporated in amounts commonly found in southern Australian wheat growing regions, contribute only a little to soil available N and to crop N uptake, even in the first year of their decomposition. Thus mature first crops of wheat, although varying greatly in dry matter yield (2.9-fold) and total N uptake (2.4-fold), took up only 27.8 and 20.2% of the legume N applied at 48.4 kg ha^?1, these corresponding to 6.1 and 10.8% of the N of the wheat crops. The availability of N from medic residues to a second wheat crop declines to <5% of input. For both first and second wheat crops, uptake of N from legume residues was approximately proportional to legume N input over the range 24.2 to 96.8 kg ha ^?1.The proportional contributions of medic N to soil inorganic N, N released in mineralization tests, and to wheat crop N, differed between seasons and soils, but for a given crop did not significantly differ between tillering, flowering and maturity. In both years, grain accounted for 52–65% of the total ¹⁵N of first crops, roots for < 5–6%. In neither year did the amounts of N or ¹⁵N in the tops change significantly between flowering and maturity, despite a gain in tops dry matter in 1979; by contrast N and ¹⁵N of roots decreased significantly during ripening in both years. Wheat plants at tillering contained about 75% of the N and ¹⁵N taken up at flowering. The amounts of legume-derived ¹⁵N in mature first wheat crops were equivalent to 82–88% of the amounts of inorganic ¹⁵N in the soil profiles at sowing. Wheat straw added at the rate of 2.5 t ha^?1, 2 months before sowing, decreased the uptake of N (15%) and ¹⁵N (18%) by wheat in a nitrogen responsive season.     

Effect of one phosphorus rate placed in different soil volumes on p uptake and growth of wheat

Abstract

The volume of soil treated with P fertilizer affects P uptake by the crop. Earlier studies have shown that the stimulation of root growth in P‐fertilized soil was similar for both corn (Zea mays L.) and soybean (Glycine max L. Merr). The objective of this research was to determine the effect of fertilizer P placement on P uptake and shoot and root growth of spring wheat (Triticum vulgare L.). Wheat was grown for 34 days in Raub silt loam (Aquic Argiudolls) in a controlled climate chamber. One rate of phosphate per pot, 150 mg P per three kg of soil, was mixed with 2, 5, 10, 20, 40 and 100% of the soil in the pot. The P was equilibrated with moist soil for 5 days at 70°C followed by 21 days at 25° C before transplanting 8‐day‐old wheat plants into each 3 L pot. The P stimulation of root growth in the P‐treated soil was similar to that for corn and soybeans. The effect could be described by the equation y = x^0.7 where y is the fraction of the root system in the P‐fertilized soil where P is mixed with x fraction of the soil. The greatest P uptake and plant growth occurred when added P was mixed with 20% of the soil.     

Chemical changes and phosphorus release during decomposition of pea residues in soil

To study C chemistry and nutrient dynamics in decomposing residues and P dynamics at the residue-soil interface, young pea (Pea-Y) and mature pea (Pea-M) residues were incubated in a sandy soil with low P availability. The study was conducted in microcosms in which the residues were separated from the soil by a nylon mesh. Controls consisted of microcosms without residues. Residues and the soil in the immediate vicinity of the nylon mesh were sampled after 5, 15, 28, 42 and 61 days. Residue chemistry was studied by ¹³C nuclear magnetic resonance (NMR) spectroscopy and determination of C, N and P concentrations. Compared to Pea-M, Pea-Y was characterised by higher N and P concentrations, higher percentage of proteins, esters, fatty acids and sugars, and was more easily decomposable in the first 15 days. Pea-M residues had a greater percentage of cellulose and other polysaccharides than Pea-Y and showed a more gradual loss in dry weight. Differences in C chemistry and N and P concentration between the residues decreased with time. The decomposition of Pea-Y and Pea-M residues resulted in an increase in microbial P in the residue-soil interface compared to the control, but available P was increased only in the vicinity of Pea-Y residues.     

Grain legume pre-crops and their residues affect the growth, P uptake and size of P pools in the rhizosphere of the following wheat

Legumes have been shown to increase P uptake of the following cereal, but the underlying mechanisms are unclear. The aim of this study was to compare the effect of legume pre-crops and their residues on the growth, P uptake and size of soil P pools in the rhizosphere of the following wheat. Three grain legumes (faba bean, chickpea and white lupin) were grown until maturity in loamy sand soil with low P availability to which 80?mg P kg^?1 was supplied. This pre-crop soil was then amended with legume residues or left un-amended and planted with wheat. The growth, P uptake and concentrations of P pools in the rhizosphere of the following wheat were measured 6?weeks after sowing. In a separate experiment, residue decomposition was measured over 42?days by determining soil CO₂ release as well as available N and P. Decomposition rates were highest for chickpea residues and lowest for wheat residues. P release was greatest from white lupin residues and N release was greatest from faba bean residues, while wheat residues resulted in net N and P immobilisation. The growth of the following wheat was greater in legume pre-crop soil without residue than in soils with residue addition, while the reverse was true for plant P concentration. Among the legumes, faba bean had the strongest effect on growth, P uptake and concentrations of the rhizosphere P pools of the following wheat. Regardless of the pre-crop and residue treatment, wheat depleted the less labile pools residual P as well as NaOH-Pi and Po, with a stronger depletion of the organic pool. We conclude that although P in the added residues may become available during decomposition, the presence of the residues in the soil had a negative effect on the growth of the following wheat. Further, pre-crops or their residues had little effect on the size of P pools in the rhizosphere of wheat.     

不同豆科植物对黄土高原弃土场的改良效果

为了探究豆科植物种植初期对黄土高原地区弃土场的改良效果,本文采用野外模拟弃土场和实验室测定相结合的方法,在弃土场上种植柠条、白三叶、红豆草、草木樨和紫花苜蓿5种豆科植物,研究1年后弃土场土壤的水分和养分状况.结果表明:各小区种植植物1年后,土壤水分状况有了明显的改善;土壤养分硝态氮质量分数增加,有机质、速效钾的质量分数除了草木樨有所增加以外,其他均减少,速效磷的质量分数均减少,土壤中的速效钾由于植物生长消耗而减少,但其质量分数丰富,足以满足植物生长的需要;土壤中的铵态氮和硝态氮质量分数表现为表聚作用,且硝态氮的质量分数均大于铵态氮;植被对土壤的改良程度依次为白三叶＞柠条＞红豆草＞紫花苜蓿＞草木樨.因此,种植豆科植物可以有效的改善土壤的水分和氮素营养,为今后弃土场土壤改良提供一定的理论参考.     

Changes in soil pH and phosphorus availability during decomposition of cover crop residues

The aim of this study was to determine the effect of winter cover crop (CC) residues on soil pH and phosphorus (P) availability. Three incubation assays were performed in pots using two CC: vetch (V) (Vicia villosa Roth.) and oats (Oa) (Avena sativa L.). Soil samples were taken from 10 sites at 0–20-cm depth. The rate of residues were 0 (D0), 10 (D1), 20 (D2), 30 (D3), and 40 (D4) g dry matter kg^?1 soil and the soil sampling was after 10, 20, 30, 60, 90, and 120 days of incubation. Soil pH, extractable P (Pe), and soil organic matter (SOM) and its fractions were determined. The pH increase was correlated with the rate applied (D1 < D2 < D3 < D4). No differences were found for pH comparing V and Oa residues with low residue rates. Soil pH changes were dependent from initial pH and SOM fractions in different soils across the incubation period. The multiple regression models showed that the pH changes were dependent on initial pH level and SOM fractions with a high R² (0.81). CC residues and its quantities produced different changes on pH – especially at the beginning of the incubation – which influenced the P availability.     

Quantitative conformation of dimethoate residues in wheat plants by single ion mass spectrometry.

A gas chromatographic-single ion mass spectrometric method was developed for determining dimethoate residues in wheat plants. The base peak (m/e 37) of dimethoate was chosen as the single ion peak, and methyl stearate was used as an internal standard for this analysis. The minimum detectable concentration of dimethoate by this method was about 0.1 ppm for a 20 g wheat plant sample. The recoveries of dimethoate were about 89% at 0.13 ppm and greater than 96% at 0.5-1 ppm.     

Physical characterization of a soil amended with organic residues in a rice–wheat cropping system using a single value soil physical index

The effect of soil incorporations of lantana (Lantana spp.) biomass, an obnoxious weed, on physical environment of a silty clay loam soil (Typic Hapludalf) under rice (Oryza sativa L.)–wheat (Triticum aestivum L.) cropping was studied in a long-term field experiment conducted in a wet temperate region of north India. Fresh lantana biomass was incorporated into the plough layer at 10, 20 and 30 Mg ha⁻¹ annually, 7–10 days before puddling. Plant-available water capacity (PAWC), non-limiting water range (NLWR) and NLWR:PAWC ratio were determined to characterize soil physical environment during wheat crop in the tenth cropping cycle.

Ten annual applications of lantana at 10, 20 and 30 Mg ha⁻¹, increased organic carbon (OC) content over control by 12.6, 17.6 and 27.9% in 0–15 cm soil layer, and 17.1, 26.3 and 39.5% in 15–30 cm soil layer, respectively. The OC content in 0–15 and 15–30 cm soil layer of control plots was 11.1 and 7.6 g kg⁻¹ soil. Bulk density decreased by 3–14% in 7.5–10.5 cm layer and 1–6% in 15–18 cm layer. Volumetric moisture contents at 10% air-filled porosity were 38.4, 40.0, 54.5 and 55.7% at 7.5–10.5 cm depth, and 31.4, 32.2, 33.9 and 34.6% at 15–18 cm depth corresponding to 0, 10, 20 and 30 Mg ha⁻¹ lantana treatment, respectively. At 15–18 cm soil depth, volumetric moisture contents at 2 MPa soil penetration resistance were 26.9, 24.8, 23.0 and 19.6% in zero, 10, 20 and 30 Mg ha⁻¹ lantana-treated plots, respectively. Lower soil water contents associated with 10% air-filled porosity and greater soil water contents associated with a limiting penetration resistance of 2 MPa resulted in a lower NLWR (4.3%) for control as compared to lantana-treated soil (7.4–15.1%). The PAWC showed slight increase from 12.9 to 13.4–14.9% due to lantana additions. The NLWR:PAWC ratio was also lower in control (0.33) as compared to lantana-treated soil (0.55–1.01). The NLWR was significantly and positively correlated with wheat grain yield (r=0.858^**).     

Response of greenhouse cucumber to mineral fertilizers on a high phosphorus and potassium soil

The response of greenhouse cucumber (Cucumis sativus L. cv. Lolita) to nitrogen (N), phosphorus (P) and potassium (K) fertilizers on a soil high in available P and K was studied during 1986. The greenhouses were located in the Beqa Valley, central Lebanon, and their soil chemical properties before planting were: NO₃‐N = 52 ppm, P(NaHCO₃ ext.) = 100 ppm, K (ammonium acetate ext.) = 650 ppm, ECe = 1.6 dS/m, pH = 7.5. Nitrogen at 200 kg/ha, P at 85 kg/ha and K at 150 kg/ha were applied in the following combinations: N, N+K, N+P+K and an unfertilized control. The rates were split into four equal weekly applications starting on the fourth week after transplanting the seedlings to the greenhouse. The treatments were applied through the drip irrigation system of the greenhouses. Fruit yield over the two months of harvest was highest in plants receiving N alone, which yielded 57 ton/ha. Yields of the plots receiving N+K, N+P+K and the control were 55.0, 54.0 and 39.5 ton/ha, respectively. Yield during the first month of harvest was comparable in all fertilized treatments and was substantially higher than the control.     

Mineralisation of crop residues on the soil surface or incorporated in the soil under controlled conditions

In the present work, we compare the effect of mature crop residues mixed into a ferralitic soil or placed as a single layer on soil surface on the mineralisation of C and N over 55 days. As residues, we used dry stems of rice, soybean, sorghum, brachiaria and wheat. There were no significant effects of residue placement on C mineralisation kinetics. Decomposition of the residues on the soil surface slightly increased net N mineralisation for residues having the smallest C/N ratio.     

Carbon and nitrogen mineralization dynamics in different soils of the tropics amended with legume residues and contrasting soil moisture contents

Seasonal drought in tropical agroecosystems may affect C and N mineralization of organic residues. To understand this effect, C and N mineralization dynamics in three tropical soils (Af, An₁, and An₂) amended with haricot bean (HB; Phaseolus vulgaris L.) and pigeon pea (PP; Cajanus cajan L.) residues (each at 5 mg g⁻¹ dry soil) at two contrasting soil moisture contents (pF2.5 and pF3.9) were investigated under laboratory incubation for 100–135 days. The legume residues markedly enhanced the net cumulative CO₂–C flux and its rate throughout the incubation period. The cumulative CO₂–C fluxes and their rates were lower at pF3.9 than at pF2.5 with control soils and also relatively lower with HB-treated than PP-treated soil samples. After 100 days of incubation, 32–42% of the amended C of residues was recovered as CO₂–C. In one of the three soils (An₁), the results revealed that the decomposition of the recalcitrant fraction was more inhibited by drought stress than easily degradable fraction, suggesting further studies of moisture stress and litter quality interactions. Significantly (p < 0.05) greater NH₄⁺–N and NO₃⁻–N were produced with PP-treated (C/N ratio, 20.4) than HB-treated (C/N ratio, 40.6) soil samples. Greater net N mineralization or lower immobilization was displayed at pF2.5 than at pF3.9 with all soil samples. Strikingly, N was immobilized equivocally in both NH₄⁺–N and NO₃⁻–N forms, challenging the paradigm that ammonium is the preferred N source for microorganisms. The results strongly exhibited altered C/N stoichiometry due to drought stress substantially affecting the active microbial functional groups, fungi being dominant over bacteria. Interestingly, the results showed that legume residues can be potential fertilizer sources for nutrient-depleted tropical soils. In addition, application of plant residue can help to counter the N loss caused by leaching. It can also synchronize crop N uptake and N release from soil by utilizing microbes as an ephemeral nutrient pool during the early crop growth period.     

Role of carrier-based biofertilizer in reclamation of saline soil and wheat growth

Two plant growth promoting rhizobacteria (PGPR), Pseudomonas moraviensis and Bacillus cereus, were used as bioinoculants on wheat, applied alone and in combination. Ground maize straw and sugarcane husk were used as carriers. Experiment was conducted for two consecutive years (2010 and 2011) under axenic conditions in the greenhouse of Quaid-e-Azam University, Islamabad. Sodium chloride (NaCl) (150 mM) was applied with irrigated water after 7 and 14 days of seed germination. Measurements made 40 days after sowing (DAS) revealed that P. moraviensis and B. cereus have better survival efficiency (as evidenced by higher colony forming units (CFUs)) in the carriers. The substantial increase in CFU of both PGPR was also observed in the soil at 57 DAS. Coinoculation of PGPR with both the carrier materials significantly decreased electrical conductivity (EC) and Na⁺ content of soil over control. The N, P, K⁺, Ca⁺, and Mg⁺ contents were 30–40% higher in soil, and 30–45% higher in leaves. Coinoculation of PGPR with carriers significantly increased chlorophyll, protein, sugar, phytohormone contents, and antioxidant activities of leaves. The application of biofertilizers improved the yield of wheat by 15–25% over control. It is inferred that the carriers assisted PGPR for long-time survival, and the formulation was applicable in promoting crop production under salt stress.     

Early nitrogen deficiency favors high nitrogen recovery efficiency by improving deeper soil root growth and reducing nitrogen loss in wheat

ABSTRACT

A two-year field and micro-plot ¹⁵N-labelled experiment was conducted under two levels of N application rate (240 and 180 kg N ha^–1) with three basal N application stages [seeding (L0), four-leaf stage (L4), and six-leaf stage (L6)] to investigate the effects of reducing basal N application amount and postponing basal N fertilization period on wheat growth and N use efficiency (NUE). No significant differences were observed in grain yield, root growth and root morphology between the N180L4 and N240L0 treatments, while the root-shoot ratio of N180L4 was significantly improved. Postponing basal N application period increased the residual basal ¹⁵N in soil and reduced basal ¹⁵N loss, and N180L4 treatment favored the highest ¹⁵N recovery efficiency (NRE), mainly due to reduced ¹⁵N loss. Grain yield and basal NRE were significantly positively correlated with root dry weight in deeper soil layers (40–60 cm), and the contribution of root growth to improved grain yield and NRE increased with the downward distribution of the roots. Therefore, postponing the basal N fertilization period under N deficiency promotes deeper root growth during the post-jointing period and increases basal N uptake, as well as reducing basal N loss and increasing grain yield and NUE.     

Arbuscular mycorrhizal fungi improve the growth and phosphorus uptake of mung bean plants fertilized with composted rock phosphate fed dung in alkaline soil environment

Abstract

Inoculation effect of arbuscular mycorrhizal fungi (AMF) on phosphorus (P) transfer from composted dung of cattle with a diet supplemented with powdered rock phosphate (RP) and their successive uptake by mung bean plants was assessed in alkaline soil. The efficacy of composted RP fed dung alone or/and in combination with AMF inoculums containing six different species were compared with SSP in six replicates per treatment in pots. The results showed that the association of AMF with composted RP fed dung had a positive effect on mung bean shoot (3.04?g) and root (2.62?g) biomass, chlorophyll (a, b), carotenoid contents and N (58.38?mg plant^?1) and P (4.61?mg plant^?1) uptake. Similarly, the percent roots colonization (56%) and nodulation of mung bean plant roots and their post-harvest soil properties were also improved by the inoculation of AMF together with composted RP fed dung. It is concluded that the combined application of AMF with composted RP fed dung has almost the same effect as SSP for improving mung bean plants growth and their nutrients uptake. Moreover, AMF inoculants can be used as a suitable biofertilizer in combination with locally available organic sources of fertilizers for improving P status and growth of plants in alkaline soils.     

Use of a microtitre plate reader in soil phosphorus analysis

Abstract

A technique is described for using microtitre plates and plate reader in soil phosphorus analysis. The molybdate‐vanadate method of phosphorus analysis was modified to use a reduced volume of reagents and each sample was read at 405 nm instead of 420 nm. Using standard microtitre plates instead of test tubes, the volume of reagent was reduced 20 X and as many as 96 samples could be read in a total of two minutes.