Microsatellite markers for genes lr34/yr18 and other quantitative trait Loci for leaf rust and stripe rust resistance in bread wheat

ABSTRACT Leaf rust and stripe rust, caused by Puccinia triticina and P. striiformis, respectively, are important diseases of wheat in many countries. In this study we sought to identify molecular markers for adult plant resistance genes that could aid in incorporating such durable resistance into wheat. We used a doubled haploid population from a Japanese cv. Fukuho-komugi x Israeli wheat Oligoculm cross that had segregated for resistance to leaf rust and stripe rust in field trials. Joint and/or single-year analyses by composite interval mapping identified two quantitative trait loci (QTL) that reduced leaf rust severity and up to 11 and 7 QTLs that might have influenced stripe rust severity and infection type, respectively. Four common QTLs reduced stripe rust severity and infection type. Except for a QTL on chromosome 7DS, no common QTL for leaf rust and stripe rust was detected. QTL-7DS derived from 'Fukuho-komugi' had the largest effect on both leaf rust and stripe rust severities, possibly due to linked resistance genes Lr34/Yr18. The microsatellite locus Xgwm295.1, located almost at the peak of the likelihood ratio contours for both leaf and stripe rust severity, was closest to Lr34/Yr18. QTLs located on 1BL for leaf rust severity and 3BS for stripe rust infection type were derived from 'Oligoculm' and considered to be due to genes Lr46 and Yr30, respectively. Most of the remaining QTLs for stripe rust severity or infection type had smaller effects. Our results indicate there is significant diversity for genes that have minor effects on stripe rust resistance, and that successful detection of these QTLs by molecular markers should be helpful both for characterizing wheat genotypes effectively and combining such resistance genes.     

Identification, Chromosome Location, and Diagnostic Markers for a New Gene (YrCN19) for Resistance to Wheat Stripe Rust

ABSTRACT Several wheat lines and cultivars of wheat (Triticum aestivum) originating from the southwestern region of China were found to be highly resistant to stripe rust by inoculation with the prevalent races (CYR30, CYR31, and CYR32) and newly emerged races (H46-4, SY11-4 and SY11-14) of the pathogen. An inheritance study of the resistance to stripe rust was carried out by crossing resistant AIM6 with susceptible BeiZ76. Results indicated that the resistance to stripe rust was controlled by a single dominant gene. The 112 F(2) plants chosen from the cross BeiZ76/ AIM6 were analyzed with 218 pairs of microsatellite primers to determine the map location of the resistance gene. A simple sequence repeat marker on chromosome arm 2BS, Xgwm410, showed polymorphism and co-segregation between stripe rust resistant and susceptible plants. From the pedigree, inheritance, molecular marker, and resistance response, it is concluded that the stripe rust resistance gene in wheat cv. Chuan-nong19 (CN19) and wheat lines AIM5 and AIM6 is a novel gene, designated YrCN19. The microsatellite primer Xgwm410 is a diagnostic marker of the resistance gene YrCN19, which has potential for application in the marker-assisted breeding of wheat.     

小麦品系中梁93444的抗条锈性遗传分析与分子作图

为明确抗锈品种中梁93444抗条锈基因及遗传特点,用CYR30、CYR31、CYR32对该品种、铭贤169及杂交组合进行遗传分析,用SSR技术对分离家系F_3-3进行PCR扩增和电泳分析。结果显示,中梁93444对CYR30、CYR31的抗病性均由1对显性和1对隐性基因控制,对CYR32由2对显性互补基因控制;F_3-3分离家系对CYR32的抗病性由1对显性基因控制,该基因暂命名为Yr93444。对F_3-3分离群体进行SSR标记,建立了与该基因连锁的8个标记Xgwm122、Xwmc702、Xwmc644、Xwmc794、Xgwm328、Xwmc455、Xgwm372、Xwmc819,遗传距离分别为38.1、30.7、22.9、15.6、10.0、6.9、3.5和2.8 cM。 应用SSR标记、中国春及缺四体将Yr93444定位于2AL上。系谱分析和SSR分子标记检测表明,该基因是来自中间偃麦草的新抗条锈基因。用与该基因紧密连锁的SSR标记Xgwm372和Xwmc819检测中梁品种和黄淮麦区主栽品种,发现89%中梁品种含该抗病基因,而86%黄淮麦区主栽品种不含该抗病基因,表明该基因应用潜力很大。     

Lr46: a gene conferring slow-rusting resistance to leaf rust in wheat

ABSTRACT Wheat (Triticum aestivum) cultivar Pavon 76 carries slow-rusting resistance to leaf rust that has remained effective in Mexico since its release in 1976. 'Pavon 76' was crossed with two leaf rust-susceptible wheat cultivars, Jupateco 73S and Avocet S, and between 118 and 148 individual F(2) plant-derived F(3) and F(5) lines were evaluated for adult-plant leaf rust resistance at two field sites in Mexico during different seasons. Evaluation of F(1) plants and parents indicated that the slow-rusting resistance was partially dominant. Segregation in the F(3) and F(5) indicated that the resistance was based on two genes with additive effects. Monosomic analysis was carried out to determine the chromosomal locations of the resistance genes. For this purpose, two or three backcross-derived cytogenetic populations were developed by crossing 'Pavon 76' with a monosomic series of adult-plant leaf rust-susceptible cultivar Lal-bahadur. Evaluation of such BC(2)F(3) and BC(3)F(3) lines from 16 confirmed 'Lalbahadur' monosomics indicated that one slow-rusting gene was located in chromosome 1B of 'Pavon 76'. This gene, designated as Lr46, is the second named gene involved in slow-rusting resistance to leaf rust in wheat.     

Tagging and validation of a major quantitative trait locus for leaf rust resistance and leaf tip necrosis in winter wheat cultivar forno

ABSTRACT A major leaf rust (Puccinia triticina) resistance quantitative trait locus (QTL) (QLrP.sfr-7DS) previously has been described on chromosome 7DS in the winter wheat (Triticum aestivum) cv. Forno. It was detected in a population of single-seed descent (SSD) lines derived from the cross Arina x Forno. QLrP.sfr-7DS conferred a durable and slow-rusting resistance phenotype, co-segregated with a QTL for leaf tip necrosis (LTN) and was mapped close to Xgwm295 at a very similar location as the adult plant leaf rust resistance gene Lr34 found in some spring wheat lines. Here, we describe the validation of this QTL by mapping it to the same chromosomal region close to Xgwm295 on chromosome 7DS in a population of SSD lines from the winter wheat x spelt (T. spelta) cross Forno x Oberkulmer. In both populations, the log of the likelihood ratio curves for leaf rust resistance and LTN peaked at identical or very similar locations, indicating that both traits are due to the same gene. We have improved the genetic map in the target region of QLrP.sfr-7DS using microsatellite and expressed sequence tag (EST) markers. Two EST loci (Xsfr.BF473324 and Xsfr.BE493812) define a genetic interval of 7.6 centimorgans containing QLrP.sfr-7DS, a considerably more precise genetic location for this QTL than previously described both in spring and winter wheat. The identified genetic interval is physically located in the distal 39% of chromosome 7DS. Single-marker analysis identified Xsfr.BF473324 and Xgwm1220 as the most informative loci for QLrP.sfr-7DS and QLtn.sfr-7DS. In the rice genome, the two ESTs flanking the QLrP.sfr-7DS/QLtn.sfr-7DS chromosomal segment in wheat are conserved on chromosome 6S in a region colinear with wheat chromosome 7DS. There, they define a physical region of three rice bacterial artificial chromosomes spanning approximately 300 kb.     

小麦品种天选43抗条锈性遗传分析及抗病基因的分子标记

 天选43是由8845-01-01-1-1和抗源材料贵农22杂交选育而成的普通小麦品种,对我国目前所有条锈菌生理小种均表现良好抗性。为明确其抗条锈性遗传基础,本研究选用当前条锈菌流行小种CYR32和CYR33,对天选43与感病品种铭贤169杂交F₁、F₂和F₃代群体进行遗传分析,同时应用460对SSR引物对接种CYR32的天选43/铭贤169 F₂代150个单株群体进行抗病基因定位。结果表明,天选43对CYR32抗性由1对显性基因控制,而对CYR33抗性由1对隐性基因控制。筛选到10个与抗CYR32基因连锁的SSR标记Xwmc134、Xgwm413、Xbarc187、Xwmc406、Xcfd65、Xgwm18、Xbarc181、Xbarc137、Xwmc419和Xgwm230,两侧距离目的基因最近的标记为Xgwm18和Xgwm413,遗传距离分别为0.8 cM和3.4 cM,并初步将其抗病基因定位于小麦染色体1BS上,暂命名为YrTx43。基因来源、抗病遗传分析、分子标记检测及染色体位点分析表明,YrTx43很可能是与Yr24、Yr26具有等位性的抗条锈基因。     

5个持久抗条锈病小麦品种的抗性组分和遗传距离的研究

 小麦数量抗病品种Aquileja(AQ)、Libellula(LB)、Luke(LK)、Nugaines(NG)和咸农4号(XN)具有持久抗条锈病特点,本文对这5个品种的抗性组分及它们之间的遗传距离进行了研究,以铭贤169(MX)为感病对照品种。苗期室内抗性组分试验表明:LB、XN、AQ 3个品种与LK、NG 2个品种相比,前者的气孔下泡囊密度低于后者;前者的菌落长度短于后者。其中AQ和XN的吸器密度低于LK和NG而高于LB;LK和NG的气孔下泡囊密度甚至高于感病对照品种MX。田间抗性组分试验表明:5个数量抗病品种AQ、LB、LK、NG和XN的反应型(IT)、严重度(DS)、病情发展曲线下面积(AUDPC)和病斑长度(LL)值均低于感病对照品种MX;数量抗病品种AQ、LB、LK、NG、XN之间相比,AQ的IT值最高而LB最低;NG的DS和AUDPC值最高而LB最低;AQ、NG和XN的LL值高于LB和LK;AQ的病斑密度值低于NG和LK而高于XN和LB。由此可见,LK和NG没有抗侵入能力而在成株期具有抗扩展能力,LB、AQ和XN除了具有抗扩展能力外,还具有降低侵入频率能力。基于SSR DNA指纹数据的聚类分析,AQ与LB为同一类,NG与XN为同一类,LK单独为一类。由此推测,AQ或LB与其它3个数量抗病品种之间杂交的后代中可能会出现性状超亲分离,本实验室报道的AQ/NG杂交组合的结果证明了这一点。本文为进一步的抗病遗传育种工作提供了有用信息。     

Mapping of QTL lengthening the latent period of Puccinia striiformis in winter wheat at the tillering growth stage

The winter wheat lines Luke and AQ24788-83 are respectively susceptible and slow-rusting at tillering stage to yellow (stripe) rust, caused by Puccinia striiformis f. sp. tritici (Pst). A mapping population consisting of 206 recombinant inbred lines was developed from the cross Luke?×?AQ24788-83. These lines were evaluated at the tillering stage in the field trials for infection type (IT) and disease incidence (DI) and in greenhouse trials for IT and latent period (LP). A significant negative correlation was found between LP and DI. A genetic map with 473 marker loci was constructed and used for identifying QTL associated with LP and IT. Two QTL, QYr.cau-1BS and QYr.cau-5AS, were mapped on 1BS and 5AS respectively, explaining collectively up to 46.4 % of LP phenotypic variance. QYr.cau-5AS was clearly distinct, in terms of mapping position, from all six yellow rust resistance genes/QTL previously reported on 5A. QYr.cau-1BS could not be spatially differentiated from three (i.e. YrAlp, Yr15, and YrH52) of the six genes/QTL known on 1BS and centromere-vicinity regions, but was determined to be different from these three genes based on phenotype. The two QTL identified here, therefore, are likely to be novel to the currently known Pst resistance genes/QTL. A minor QTL on 3AL was detected to be associated with both IT and LP. Expression of quantitative resistance at early wheat growth stages and usefulness of the QTL are discussed for the wheat-Pst system.     

Identification of a quantitative trait locus for high-temperature adult-plant resistance against Puccinia striiformis f. sp. hordei in 'Bancroft' barley

Sustainable control of plant diseases can be achieved by developing cultivars with durable resistance. 'Bancroft' barley has durable high-temperature, adult-plant (HTAP) resistance to stripe rust caused by Puccinia striiformis f. sp. hordei. The objectives of this study were to determine the inheritance of the HTAP resistance in Bancroft, develop molecular markers for the HTAP resistance using the resistance gene analog polymorphism (RGAP) technique, map the HTAP resistance quantitative trait locus or loci (QTL) on barley chromosomes, and determine the usefulness of the RGAP markers in other barley cultivars for marker-assisted selection. The parents and F(4) recombinant inbred lines (RIL) and the parents and F(5) RIL were evaluated in 2004 and 2005 in one and three field sites, respectively, in Washington State. Infection type (IT) and disease severity (DS) were recorded three times at each location during each growing season. Area under the disease progress curve (AUDPC) was calculated for each parent and RIL based on the DS data. Genetic analyses of IT data of the parents, F(1), and F(2) tested in the adult-plant stage under controlled high-temperature cycle in the greenhouse and the parents, F(4), and F(5) RIL in the field indicated that one dominant gene controlled the HTAP resistance in Bancroft. Using 119 F(5:6) RIL and IT data, a linkage map on chromosome arm 3HL was constructed with eight RGAP markers and three simple sequence repeat (SSR) markers. Using the QTL analysis, a QTL for HTAP resistance was mapped with the DS and AUDPC data on the same chromosome location as with the IT data. The QTL explained >70% of the total phenotypic variation for the DS and AUDPC. The heritability of the HTAP resistance based on the AUDPC data was 76%. The two markers most close to the QTL peak detected polymorphisms in 84 and 88% of 25 barley genotypes that do not have the Bancroft HTAP resistance when used individually, and detected polymorphism in 100% of the genotypes when used in combination, indicating that the markers could be used in incorporating the HTAP resistance into these barley genotypes to improve the level and durability of resistance to stripe rust.     

小偃9366抗条锈病基因的遗传分析及分子作图

为了明确小偃9366抗条锈病遗传特点,对小偃9366与铭贤169及其杂交F₁、F₂、F₃和BC₁F₁代进行温室苗期抗条锈性遗传分析,选取400余对SSR引物对接种CYR31的群体进行分子标记,并利用目标基因的侧翼引物分析99个黄淮麦区主栽小麦品种。小偃9366对CYR25的抗病性由1显、1隐2对基因独立控制,对CYR27的抗病性由3对显性基因控制,其中2对基因表现累加作用,对CYR30 和CYR31的抗病性均由1对显性基因独立控制,对Su11-4的抗性由2对隐性基因独立控制。位于2AL上的6个标记Xwmc794、Xwmc455、Xwmc261、Xgwm47、Xgwm294和Xcfd168与抗CYR31基因(暂命名Yrxy9366)连锁,与目的基因的遗传距离分别为10.8、6.5、3.2、4.4、16.0和32.8 cM,将Yrxy9366定位在2AL上。利用Xwmc261、Xgwm47两个引物分析99个黄淮麦区主栽小麦品种,仅5%检测到同源片段。研究表明Yrxy9366是一个新的抗病基因。     

中粱93447抗条锈病基因遗传分析和分子作图

 用小麦条锈菌生理小种对中梁93447与感病品种铭贤169的杂交后代F₁、F₂和F₃代进行苗期温室抗条锈性遗传分析,结果表明中梁93447对CYR30的抗病性由1对显性基因控制。用中梁93447×铭贤169 F₂代分离群体建立抗、感DNA池,在F₂代群体中通过SSR标记技术寻找与抗病基因连锁的分子标记,发现7个位于5BS上的标记Xwmc813、Xwmc740、Xgwm159、Xbarc4、Xwmc616、Xwmc363和Xbarc89与目的基因连锁,遗传距离分别为17、12.9、8.3、4.5、3.7、9.1和20.8cM。系谱分析及分子标记分析表明,该基因可能来自中间偃麦草,暂命名为YrZL93447。与已定位于5B染色体上的抗条锈病基因的比较研究表明,YrZL93447可能是1个新的抗条锈病基因。用SSR引物BARC4和WMC616检测43个黄淮麦区主栽品种,其中7%的黄淮麦区主栽品种具有与YrZL93447基因相同的标记位点。     

The dissection and SSR mapping of a high-temperature adult-plant stripe rust resistance gene in American spring wheat cultivar Alturas

Stripe rust is one of major diseases in wheat production worldwide. The best economic and efficient method is to utilize resistant varieties. Alturas has high-temperature adult-plant resistance. In order to determine stripe rust resistance characteristics, resistance gene combination and molecular map of the resistance gene(s), Alturas was crossed with Chinese susceptible cultivar Taichung29. The parents, F₁, F₂ progenies were tested with Chinese predominant mixed races CYR31, CYR32 and CYR33 in field experiments in 2010 and F₃ progenies were evaluated at one site in Beijing, the other site in Langfang, Hebei Province. Infection type (IT) and disease severity (DS) were recorded three times for each plant for F₁ and F₂, and each progeny for F₃ during each growing season. The DS data were used to calculate relative area under the disease progress curve (AUDPC) values. Both IT and AUDPC data showed continuous distributions, indicating that the Alturas HTAP resistance was controlled by quantitative trait loci (QTLs). A major HTAP QTL, designated as QYrAlt.syau-3BS, was consistently detected across environments and was located on chromosome 3BS. The gene contributed to 34.28?% of the phenotypic variation for average AUDPC and 50.20?% for average IT. Markers Xgwm389 and Xbarc238 flanking the major QTL, should be useful in breeding for obtaining durable and high-level resistance by combinations with other non-race-specific resistance genes.     

Quantitative trait Loci mapping for adult-plant resistance to powdery mildew in bread wheat

ABSTRACT Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a major disease to wheat (Triticum aestivum) worldwide. Use of adult-plant resistance (APR) is an effective method to develop wheat cultivars with durable resistance to powdery mildew. In the present study, 432 molecular markers were used to map quantitative trait loci (QTL) for APR to powdery mildew in a doubled haploid (DH) population with 107 lines derived from the cross Fukuho-komugi x Oligoculm. Field trials were conducted in Beijing and Anyang, China during 2003-2004 and 2004-2005 cropping seasons, respectively. The DH lines were planted in a randomized complete block design with three replicates. Artificial inoculation was carried out in Beijing with highly virulent isolate E20 of B. graminis f. sp. tritici and the powdery mildew severity on penultimate leaf was evaluated four times, and the maximum disease severity (MDS) on penultimate leaf was investigated in Anyang under natural inoculation in May 2004 and 2005. The heritability of resistance to powdery mildew for MDS in 2 years and two locations ranged from 0.82 to 0.93, while the heritability for area under the disease progress curve was between 0.84 and 0.91. With the method of composite interval mapping, four QTL for APR to powdery mildew were detected on chromosomes 1AS, 2BL, 4BL, and 7DS, explaining 5.7 to 26.6% of the phenotypic variance. Three QTL on chromosomes 1AS, 2BL, and 7DS were derived from the female, Fukuho-komugi, while the one on chromosome 4BL was from the male, Oligoculm. The QTL on chromosome 1AS showed high genetic effect on powdery mildew resistance, accounting for 19.5 to 26.6% of phenotypic variance across two environments. The QTL on 7DS associated with the locus Lr34/Yr18, flanked by microsatellite Xgwm295.1 and Ltn (leaf tip necrosis). These results will benefit for improving powdery mildew resistance in wheat breeding programs.     

小麦抗条锈病一致性数量性状位点(MQTL)图谱构建

 小麦条锈病是造成小麦减产和品质劣化的最重要病害,定位小麦染色体上一致性条锈病抗性基因/位点/区段是小麦条锈病抗性分子育种的重要基础。本研究对至今分子标记和遗传定位的342个条锈病抗性基因/位点/区段进行数据搜集整理,借助Maccaferr和Andrzej的参考图谱,基于元分析技术进行Meta-QTL(MQTL)检测,共获得194个小麦抗条锈病MQTL,包括74个与严重度(Disease severity, DS)相关,46个与反应型(Infection type, IT)相关、19个与病程曲线下面积相关(Area under disease progress curve, AUDPC)、28个与DS和IT共相关、6个与DS和AUDPC共相关、15个与IT和AUDPC共相关、6个与其他条锈病抗性性状相关。这些抗条锈病一致性QTL定位于小麦21条染色体上,呈非均匀分布,且部分MQTL集中成簇。通过与已发表的正式命名抗条锈病基因比较分析,发现大多数正式命名基因定位于MQTL簇区段,说明这些MQTL簇区段很可能是控制小麦条锈病抗性热点区域。控制小麦抗条锈病一致性QTL遗传图谱的构建为小麦条锈病抗性基因精细定位及抗病育种提供了遗传信息参考依据。     

小麦抗条锈病一致性数量性状位点(MQTL)图谱构建

 小麦条锈病是造成小麦减产和品质劣化的最重要病害,定位小麦染色体上一致性条锈病抗性基因/位点/区段是小麦条锈病抗性分子育种的重要基础。本研究对至今分子标记和遗传定位的342个条锈病抗性基因/位点/区段进行数据搜集整理,借助Maccaferr和Andrzej的参考图谱,基于元分析技术进行Meta-QTL(MQTL)检测,共获得194个小麦抗条锈病MQTL,包括74个与严重度(Disease severity, DS)相关,46个与反应型(Infection type, IT)相关、19个与病程曲线下面积相关(Area under disease progress curve, AUDPC)、28个与DS和IT共相关、6个与DS和AUDPC共相关、15个与IT和AUDPC共相关、6个与其他条锈病抗性性状相关。这些抗条锈病一致性QTL定位于小麦21条染色体上,呈非均匀分布,且部分MQTL集中成簇。通过与已发表的正式命名抗条锈病基因比较分析,发现大多数正式命名基因定位于MQTL簇区段,说明这些MQTL簇区段很可能是控制小麦条锈病抗性热点区域。控制小麦抗条锈病一致性QTL遗传图谱的构建为小麦条锈病抗性基因精细定位及抗病育种提供了遗传信息参考依据。     

人工合成小麦新种质抗条锈性鉴定与Yr18基因检测

对97份来自CIMMYT的人工合成小麦新种质进行了主要农艺性状考察、Yr18分子检测和成株期田间抗锈性接种鉴定。结果表明:参鉴种质间主要农艺性状变异程度较大,尤其千粒重普遍偏高,有30份材料千粒重达50 g以上、占30.9%;利用csLV34标记检测到合成20和合成43携带Yr18,占2.1%;鉴选出成株期呈抗性反应的材料30份、占30.9%,高度慢条锈材料19份、占19.6%,其中合成2、5、14、60、75、76、78、82、83、84计10个合成种同时表现高抗和高度慢锈。这些大粒、抗条锈或慢条锈合成小麦种质的鉴定筛选,为选育小麦新品种提供了优异资源。     

普通小麦品种陕旱8675中抗条锈病基因的分子定位

 利用SSR标记技术对小麦品种陕旱8675的抗条锈病基因进行了分子标记定位。通过对290对微卫星引物的筛选,发现Xwmc170和Xcfa20432对引物在抗亲、感亲、抗池和感池之间均有多态。群体分析结果表明,Xwmc170和Xcfa2043与陕旱8675中抗病基因相连锁,遗传距离分别为9.8 cM和15.0 cM,基因和标记之间的顺序为着丝点-Xcfa2043-Xwmc170-YrSh,间隔距离分别为19.05、5.2和9.8 cM。根据作图的结果,将陕旱8675中所含有的抗病基因定位于2A染色体长臂上,根据该基因的作图位置与抗谱分析,认为该基因可能是1个新的抗条锈基因,暂定名为YrSh。     

普通小麦-华山新麦草易位系9020-17-25-6抗条锈性遗传分析及分子标记

为明确普通小麦-华山新麦草易位系9020-17-25-6的抗条锈病基因及其遗传特点,利用中国条锈菌小种CYR29对9020-17-25-6、铭贤169及其杂交后代F1、F2、F3代进行苗期抗条锈性鉴定及遗传分析,选取48条RGAP引物和491对SSR引物对接种CYR29的F2代群体进行筛选,寻找与抗病基因连锁的分子标记。结果表明:9020-17-25-6对CYR29具有良好的抗条锈性,由1对显性基因独立控制,暂定名为Yr Hua9020。筛选到2个RGAP标记(M1和M2)和位于染色体3AS上的4个SSR标记(Xwmc11、Xwmc532、Xcfd79、Xgwm2)与Yr Hua9020连锁,与目的基因的遗传距离分别为6.9、9.5、17.8、12.2、7.2和17.8 c M。与已定位于3A染色体上的抗条锈病基因的比较研究表明,Yr Hua9020是一个与已知基因不同的新的抗条锈病基因。     

小簇麦易位系V9128-3抗条锈病基因的遗传分析和SSR分子标记

 用7个我国当前流行的条锈菌生理小种对V9128-3的抗条锈性进行了评价,表明本易位系对我国优势流行小种具有良好的抗病性。以Su-4对V9128-3与铭贤169配置的F₁、BC₁F₁、F₂及F₃代群体进行了遗传分析,并对其中1个F₂群体进行了SSR标记,再用BC₁F₁群体的部分单株和F₃家系进行连锁标记的初步验证。遗传分析表明了V9128-3对Su-4的抗病性由1对显性核基因独立控制,从219对SSR引物中筛选到2个位于2AL上的该基因YrHV(暂命名)两侧的标记Xgwm356和Xwmc658,遗传距离分别为8.5和5.6cM,所用部分BC₁F₁单株和F₃家系验证了该2个标记与YrHV连锁性。将此标记可用于小麦抗条锈病分子标记辅助育种。     

粉锈宁药效与小麦品种对条锈菌感病性关系的研究

在田间小区试验条件下,粉锈宁喷雾防治小麦条锈病,每666.6m~28克用药量对各类型感病小麦品种的效应是有显著差异的,对慢锈品种和前期中感,但抽穗后有抗性的品种均可控制为害,而对极感品种则未能控制产量损失;等比系列用药量对各类型感病品种的效应也是有明显差异的,在 ED_(50)和 ED_(95)(克/666.6m~2)平均值上,极感品种为9.38和74.85,高感品种为4.53和25.43,中感品种为3.62和20.96,慢锈品种为1.82和15.30。根据这一结果,模拟出各类型感病品种大田防治的每666.6m~2适宜用药量,高感品种9—12克,中感品种7—9克,慢锈品种4—6克,并经实践证明,这一剂量标准是可行的。拌种对压低各类型感病品种的成株期病情也有明显差异,造成这种差异的主要原因是各类型品种流行速度不同。