Nucleotide sequence of a human Blym transforming gene activated in a Burkitt's lymphoma

The nucleotide sequence of a human Blym-1 transforming gene activated in a Burkitt's lymphoma cell line was determined. This sequence predicts a small protein of 58 amino acids that is 33 percent identical to the predicted product of chicken Blym-1, the activated transforming gene of chicken B cell lymphomas. Both the human and chicken Blym-1 genes exhibit significant identity to an amino-terminal region of transferrins.     

Tissue factor gene localized to human chromosome 1 (1pter----1p21)

Tissue factor (tissue thromboplastin, coagulation factor III), a protein component of cell membranes, is an essential cofactor for factor VII-dependent initiation of blood coagulation. Since no tissue factor-deficient condition has been described, it is one of only a few proteins of the coagulation system for which the pattern of inheritance has not been ascertained. Because of the species-specificity of tissue factor activity and the availability of a very sensitive chromogenic assay, it was possible in the present study to use somatic cell hybrids to assign the chromosomal location of the tissue factor structural gene (F3) to human chromosome 1 (1pter----1p21).     

Isolation of human C-reactive protein complementary DNA and localization of the gene to chromosome 1

With a synthetic oligonucleotide mixture as probe, complementary DNA clones of C-reactive protein were isolated from an adult human liver complementary DNA library. The clones ranged in size from 700 to 1100 base pairs and were identified by partial DNA sequence analysis. One complementary DNA clone was used as a probe for hybridization with human-rodent DNA's isolated from somatic cell hybrids and bound to nitrocellulose filters (Southern blot analysis) to assign the human C-reactive protein gene to chromosome 1.     

A major human histone gene cluster on the long arm of chromosome 1

A human histone gene cluster was assigned to chromosome 1 by Southern blot analysis of DNA's from a series of mouse-human somatic cell hybrids with 32P-labeled cloned human H4 and H3 histone DNA as probes. Localization of this histone gene cluster on the long arm of chromosome 1 was confirmed by in situ hybridization of this DNA probe to metaphase chromosomes.     

利用染色体片段代换系定位一个水稻显性早熟基因

以水稻染色体片段代换系构建的分离群体为材料,利用微卫星标记(Simple sequence repeat,SSR)定位一个生育期基因,对水稻生育期基因的分子标记辅助选择进行了研究.结果表明,早熟显性基因(暂命名为Hd10-1)定位于第10染色体,SSR标记RM271和RM258之间,与RM 271的遗传距离为1.90 cM,与RM 258的遗传距离为8.40 cM,RM 271与RM 258位于Hd10-1的两侧.     

5' viral and human cellular sequences corresponding to the transforming gene of simian sarcoma virus

The 5' nucleotide sequences of the transforming gene of simian sarcoma virus (v-sis) and its human cellular homolog (c-sis) were compared. A short homology was found between helper virus and cellular DNA sequences at the junction of v-sis and c-sis, which may have had a role in the original recombination event leading to the generation of simian sarcoma virus.     

Mapping human autosomes: assignment of the MN locus to a specific segment in the long arm of chromosome no. 2

The locus for MN blood groups, MN, is tentatively assigned to a specific region near the centromere of the long arm of chromosome No. 2 on the basis of a demonstrable deletion of band 2q14 from a No. 2 chromosome in a boy previously reported to be hemizygous (M/-) at that locus.     

高赖氨酸蛋白基因遗传转化水稻的研究

采用农杆菌介导的方法将高赖氨酸蛋白基因导入台粳9号幼胚诱导的胚性愈伤组织中,经潮霉素筛选,抗性愈伤分化成苗,共获得2株转基因植株.对这些植株及后代植株进行GUS染色和PCR及PCR Southern b lot检测分析,确定高赖氨酸蛋白基因已整合到台粳9号基因组中,并能稳定遗传表达.经检测,转基因水稻糙米赖氨酸含量为0.349%,比对照提高29.3%;转基因水稻秸秆赖氨酸含量为0.341%,比对照提高8.3%.     

辽棉19号转化耐盐基因AlNHX1的研究

为提高辽宁棉花品种的耐盐性,利用农杆菌介导上胚轴外植体转化法,将来源于盐生植物獐茅的Na~+/H~+逆向转运蛋白基因(AlNHX1)导入棉花辽棉19号中,分析影响棉花上胚轴外植体农杆菌转化的几个重要因素,建立并优化了该品种的转化体系,结果表明:1)农杆菌侵染的最佳时间为90s,共培养过程中乙酰丁香酮最适浓度为100 mg/L,筛选培养基中潮霉素最适浓度为30 mg/L,生根培养基中吲哚乙酸IBA的最适浓度为10mg/L;2)对转化植株进行PCR检测,表明耐盐基因AlNHX1已导入辽棉19号中;3)在盐胁迫下,转基因植株的电导率,渗透势都显著低于未转化植株。结果表明,通过筛选并优化转化体系,辽棉19号品种的耐盐性有较明显的提升,为沿海滩涂地区种植棉花提供了一定的参考。     

Localization of the gene encoding the human interleukin-2 receptor on chromosome 10

The human interleukin-2 receptor is an inducible growth factor receptor present on the surface of activated T lymphocytes. The receptor is required for a normal T-cell immune response. High-resolution fluorescence-activated chromosome sorting and DNA spot-blot analysis with complementary DNA's for the interleukin-2 receptor indicated that the receptor gene was located on chromosome 9, 10, 11, or 12. In situ hybridization studies showed that the interleukin-2 receptor gene is on the short arm of chromosome 10, p14----15.     

Location of the c-yes gene on the human chromosome and its expression in various tissues

Analysis of DNA from human embryo fibroblasts showed that ten Eco RI fragments were hybridizable with the Yamaguchi sarcoma virus oncogene (v-yes). Four of the Eco RI fragments were assigned to chromosome 18 and one to chromosome 6. There was evidence for multiple copies of yes-related genes in the human genome; however, only a single RNA species, 4.8 kilobases in length, was related to yes in various cells.     

鸡W染色体基因GPBP1在胚胎组织中的表达研究

鸡W染色体为进化过程中保留下来的Z染色体高度退化后的拷贝,在机体发育和代谢等过程中发挥着重要作用.以鸡W染色体上富含GC启动子的结合蛋白(GPBP1)基因为对象,对其在胚胎发育过程中的表达和功能进行初步研究;根据GPBP1基因在W和Z染色体上序列的不同,设计特异性引物扩增其W拷贝(GPBP1-W)、Z拷贝(GPBP1-Z)及同时扩增W和Z拷贝(GPBP1-ZW),采用qPCR及Western blot技术分析其在胚胎发育过程中不同组织的表达量,并通过FAD诱导性反转探究其对性别表型的响应.结果 表明:GPBP1-W在孵化6d的胚胎性腺中高表达,且表达量随胚胎发育而升高.GPBP1-W和GPBP1-Z在所检测的孵化12 d的胚胎组织中均有表达,其中mRNA高表达于脾脏组织中,蛋白高表达于性腺组织中;GPBP1-Z mRNA和蛋白质在雄性组织中的表达量高于雌性.GPBP1-W表达受雌性向雄性性反转的影响而显著降低,但GPBP1-Z的表达不受性反转的影响.这一研究提示,鸡性染色体基因GPBP1在鸡胚的性腺和脾脏中表达量高,且其Z拷贝在性别间和各组织中存在剂量效应和转录后调控作用,而W拷贝可能在雌性的部分组织中作为Z拷贝的补偿以保证其生物学功能的发挥.     

Herpes-type virus and chromosome marker in normal leukocytes after growth with irradiated Burkitt cells

Cultured cells derived from male patients with Burkitt's lymphoma and harboring herpes-type virus particles were lethally irradiated. These irradiated cells induced normal peripheral leukocytes of female infants to grow within 2 to 4 weeks after mixed cultivation. Cells of a line free of this agent failed to stimulate growth. If either type of cell was cultured separately, it did not survive under the experimental conditions. Herpes-type viral antigen and C-group chromosomal marker previously described in cultured Burkitt cells were found in all of the female cell cultures that were obtained.     

The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1

Fragments of the recently cloned human gene for the beta subunit of nerve growth factor (beta-NGF) were used as hybridization probes in analyzing two sets of rodent-human somatic cell hybrids for the presence of human beta-NGF sequences. Results from the first set of hybrids assigned the human beta-NGF gene to chromosome 1 and ruled out the presence of sequences of comparable homology on any other chromosome. With the second set of hybrids, which contained seven different, but overlapping, regions of chromosome 1, the NGF locus was mapped to band 1p22.     

Induction of cellular senescence in immortalized cells by human chromosome 1

The control of cellular senescence by specific human chromosomes was examined in interspecies cell hybrids between diploid human fibroblasts and an immortal, Syrian hamster cell line. Most such hybrids exhibited a limited life span comparable to that of the human fibroblasts, indicating that cellular senescence is dominant in these hybrids. Karyotypic analyses of the hybrid clones that did not senesce revealed that all these clones had lost both copies of human chromosome 1, whereas all other human chromosomes were observed in at least some of the immortal hybrids. The application of selective pressure for retention of human chromosome 1 to the cell hybrids resulted in an increased percentage of hybrids that senesced. Further, the introduction of a single copy of human chromosome 1 to the hamster cells by microcell fusion caused typical signs of cellular senescence. Transfer of chromosome 11 had no effect on the growth of the cells. These findings indicate that human chromosome 1 may participate in the control of cellular senescence and further support a genetic basis for cellular senescence.     

Mapping of herpes simplex virus-1 neurovirulence to gamma 134.5, a gene nonessential for growth in culture

The gene designated gamma 134.5 maps in the inverted repeats flanking the long unique sequence of herpes simplex virus-1 (HSV-1) DNA, and therefore it is present in two copies per genome. This gene is not essential for viral growth in cell culture. Four recombinant viruses were genetically engineered to test the function of this gene. These were (i) a virus from which both copies of the gene were deleted, (ii) a virus containing a stop codon in both copies of the gene, (iii) a virus containing after the first codon an insert encoding a 16-amino acid epitope known to react with a specific monoclonal antibody, and (iv) a virus in which the deleted sequences were restored. The viruses from which the gene was deleted or which carried stop codons were avirulent on intracerebral inoculation of mice. The virus with the gene tagged by the sequence encoding the epitope was moderately virulent, whereas the restored virus reacquired the phenotype of the parent virus. Significant amounts of virus were recovered only from brains of animals inoculated with virulent viruses. Inasmuch as the product of the gamma 134.5 gene extended the host range of the virus by enabling it to replicate and destroy brain cells, it is a viral neurovirulence factor.     

菜豆几丁质酶基因转化小麦的研究

利用农杆菌二元载体转化法和花粉管通道法 ,以菜豆几丁质酶基因转化小麦东农 774 2。农杆菌感染后的愈伤组织以 G4 183 0 mg· L-1筛选 5周 ,经 PCR和 PCR- Southern杂交检测 ,有0 .3 8%的愈伤获得转化。花粉管通道法转化小麦共操作小花 3 85朵 ,其中有 68.3 0 %的小花结实 ,经 PCR和 PCR- Southern杂交检测 ,获得转基因植株的转化频率为 0 .52 %。     

Three distinct genes in human DNA related to the transforming genes of mammalian sarcoma retroviruses

Southern blot hybridization was used to identify human and other vertebrate DNA sequences that were homologous to cloned DNA fragments containing the oncogenic nucleic acid sequences of three different type C mammalian retroviruses (simian sarcoma virus, the Snyder-Theilen strain of feline sarcoma virus, and the Harvey strain of murine sarcoma virus). Each onc gene counterpart has a single genetic locus, which probably contains non-onc intervening sequences. The human DNA sequences may represent genes important to cell growth or cell differentiation, or both. Their identification and isolation may allow elucidation of their role in these processes and in neoplasias.