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1.
Background: A study was undertaken to examine the effects of poultry fat (PF) compared with those of soybean oil (SBO) on intestinal development, fatty acid transporter protein (FATP) mRNA expression, and fatty acid composition in broiler chickens. A total of 144 day-old male commercial broilers were randomly allocated to 2 treatment groups (6 replicates of 12 chicks for each treatment) and fed isocaloric diets containing 3.0% PF or 2.7% SBO at 0 to 3 wk and 3.8% PF or 3.5% SBO at 4 to 6 wk, respectively. Results: PF had no influence on intestinal morphology, weight, or DNA, RNA, or protein concentrations at 2, 4, and 6 wk of age. However, compared with SBO, PF significantly decreased FATP mRNA abundance at 4 wk (P=0.009) and 6 wk of age (P<0.001); decreased liver fatty acid-binding protein (L-FABP) mRNA abundance at 6 wk of age (P=0.039); and decreased C18:2 (P=0.015), C18:3 (P<0.001), C20:2 (P=0.018), Σ-polyunsaturated fatty acids (Σ-PUFA) (P = 0.020), and the proportion of PUFA (P<0.001) in the intestinal mucosa and decreased C18:2 (P=0.010), C18:3 (P<0.001), C20:2 (P<0.001), Σ-PUFA (P=0.005), and the proportion of PUFA (P<0.001) in breast muscle at 6 wk of age. Conclusions: PF decreases FATP and L-FABP mRNA expression and decreased the proportion of PUFA in the intestinal mucosa and breast muscle. 相似文献
3.
The effect of fat source on fat and fatty acid (FA) apparent faecal (aFD) and ileal digestibility (aID) was studied in growing pigs. Faecal and ileal digestibilities were measured, using titanium dioxide as inert marker, in intact and ileo-rectally anastomosed pigs, respectively. Five different fat sources, added at 10% to a barley based diet (B), were tested: tallow (T), high oleic sunflower oil (HOSF), sunflower oil (SO), linseed oil (LO) and a fat blend (FB; 5.5% T, 3.5% SO and 1% LO of diet). Except for B and T, fat aFD and aID were relatively similar among diets and site of measurements and the same was observed when it was obtained from the sum of FA aFD. However fat and sum of FA aID varied according to dietary FA composition and it was inversely related with the saturated FA content, due to the lowest aID of palmitic and stearic. aID of linoleic in SO and linolenic in LO were higher than the respective FA of the other diets and no differences were observed for oleic acid. The aFD of the unsaturated FA was higher than the corresponding aID values, despite similar fat aID and aFD. This together with the lower (in some cases negative) aFD of stearic acid suggest that there is biohydrogenation of unsaturated FA in the hindgut. Measurement at the end of the ileum should give a better estimation of digestibility of fat and FA than at the faecal level. 相似文献
7.
Fatty acid transport protein 1 (FATP1) plays an important role in the fatty acid transmembrane transport and fat deposition. However, its role in porcine intramuscular preadipocytes proliferation and differentiation remain poorly understood. Here, we examined the effects of pFATP1 on porcine intramuscular preadipocytes proliferation and differentiation. Overexpression of pFATP1 in porcine intramuscular preadipocytes significantly promoted the proliferation of porcine intramuscular preadipocytes, and also significantly upregulated the expressions of peroxisome proliferator‐activated receptor γ, CCAAT enhancer binding protein α, lipoprotein lipase, fatty acid synthetase and perilipin 1. Moreover, overexpression of pFATP1 in porcine intramuscular preadipocytes significantly increased fat accumulation and downregulated β‐catenin protein expression. Overall, our results indicated that pFATP1 played an important role in porcine intramuscular preadipocytes proliferation and differentiation, and it might promote adipogenesis in porcine intramuscular preadipocytes by repressing Wnt/β‐catenin signaling pathway. 相似文献
8.
The purpose of this work was to evaluate the fatty acid composition of the Longissimus muscle from carcasses of Nellore steers fed diets with calcium salts of fatty acids (CSFA) and high moisture corn. Forty eight steers were fed during 70 days four diets containing dry corn (DC), high moisture corn (HM), dry corn plus CSFA (DC-CSFA) or high moisture corn plus CSFA (HM-CSFA). Fatty acid composition of the Longissimus muscle was determined by gas chromatography. Corn type had no effect on the ether extract percentage and in the content of the majority of the fatty acids, although steers fed HMC showed higher levels of polyunsaturated fatty acids and polyunsaturated/saturated ratio. Feeding CSFA increased ether extract percentage but had no effect on total of saturated, unsaturated and saturated: unsaturated ratio. Both high moisture corn and calcium salts of fatty acids increased CLA (cis9, trans11) and total CLA concentrations in intramuscular fat. 相似文献
9.
该试验旨在评价2种不同脂肪来源的饲料中添加菊粉对肉鸡行为、血清代谢物、肝脏脂质、腹脂、胸肌和腿肌中脂肪酸组成的影响。将240只1日龄雌性肉鸡随机分成6组,每组8个重复,每个重复5只肉鸡。试验采用3×2双因子试验设计,菊粉设3个水平(0、5和10g/kg),日粮中2种类型的脂肪[棕榈油(PO)组和葵花籽油(SO)组]含量均为90g/kg。试验期为1~34d。日粮中的脂肪不影响肉鸡的体重增长,但PO组肉鸡的消化率低于SO组(p〈0.001)。日粮中含棕榈油增加了肉鸡腹脂沉积和血清中脂质及葡萄糖的含量。PO组肉鸡肝脏中的三酰甘油含量高于SO组。同样,日粮中的脂肪改变了肉鸡腹脂和肌内的脂肪酸组成,导致PO组肉鸡的C16:0和C18:1n-9含量高于SO组,C18:2n-6含量却更低。日粮中添加菊粉对肉鸡体重的增长(p=0.017)有二次效应,而对消化率没有影响。与对照组相比,菊粉减少了肉鸡肝脏油脂的总含量(p=0.003)、血清中甘油三酯含量以及低密度的脂蛋白胆固醇(31%)。菊粉添加到含葵花籽油的日粮中,增加了肉鸡腹脂和肌内多不饱和脂肪酸与饱和脂肪酸的比值。该研究结果表明:日粮中添加菊粉对肉鸡血脂的有益作用,是因为减少了三酰甘油含量。该结果同样证明,菊粉增强了葵花籽油提高肉鸡肌内多不饱和脂肪酸与饱和脂肪酸比值的能力。 相似文献
10.
BackgroundHamburger is the most consumed beef product in North America, but lacks in nutritional appeal due to its high fat content and high proportion of saturated fatty acids (SFA). Objectives of the present study were to improve the FA profiles of hamburgers made with perirenal fat (PRF) and subcutaneous fat (SCF) when feeding steers different diets along with examining differences in sensory attributes and oxidative stability. Diets included a control diet containing 70:30 red clover silage: barley based concentrate, a diet containing sunflower-seed (SS) substituted for barley, and diets containing SS with 15% wheat dried distillers’ grain with solubles (DDGS-15) or 30% DDGS (DDGS-30). Hamburgers were made from triceps brachii and either PRF or SCF (80:20 w/w). ResultsPerirenal fat versus SCF hamburgers FA had 14.3% more ( P <0.05) 18:0, 11.8% less cis ( c)9-18:1 ( P <0.05), and 1.82% more total trans ( t)-18:1 mainly in the form of t11-18:1. During sensory evaluation, PRF versus SCF hamburgers had greater ( P <0.05) mouth coating, but the difference was less than one panel unit. Examining effects of steer diet within PRF hamburgers, feeding the SS compared to the control diet increased ( P <0.05) t-18:1 by 2.89% mainly in the form of t11-18:1, feeding DGGS-15 diet led to no further changes ( P >0.05), but feeding DDGS-30 diet reduced the proportions of ( P <0.05) of t-18:1 chiefly t11-18:1. Feeding SS and DDGS diets had small but significant ( P <0.05) effects on hamburger sensory attributes and oxidative stability. ConclusionsFeeding high-forage diets including SS and 15% DDGS, and taking advantage of the FA heterogeneity between fat depots offers an opportunity to differentially enhance beef hamburgers with 18:2 n-6 biohydrogenation products ( i.e., t11-18:1) with potential human health benefits without compromising their sensory attributes and oxidative stability during retail display. 相似文献
11.
This experiment was designed to examine changes in milk fatty acids during fish oil-induced milk fat depression (MFD) and to test the theory that these changes are related to milk fat fluidity. The experiment was divided into three periods: 1) Baseline: all cows ( n = 12) received a high fiber diet without fish oil (FO) for 12 days; 2) Treatment: 4 cows/group received the following treatments for 21 days: a) Low fiber diet without FO (LF), b) High fiber diet + FO (HF + FO) and c) Low fiber diet + FO (LF + FO); 3) Post-treatment: cows returned to the baseline diet and were monitored for 12 days. FO was included at 1.6% DM and HF and LF diets had 40 and 26% NDF, respectively. Milk fat content and yield were unchanged by the LF diet, but were reduced by FO diets at both dietary fiber levels and recovered in the post-treatment period. FO diets caused a pronounced reduction in stearic and oleic acid concentrations in milk fat and an equally pronounced increase in trans-18:1 fatty acid concentrations. Milk fat mean melting point (MMP) was correlated with MFD ( r = 0.73) and with milk oleic acid concentration ( r = − 0.92). The ratio of oleic:stearic in milk fat increased gradually and consistently in response to FO. Trans-C18:1 isomers with double bounds at carbon ≤ 10 increased with greater MFD and those with double bonds at carbon ≥ 11 decreased with greater MFD. Trans-9 cis-11 CLA explained more than 80% of MFD and was strongly correlated with trans-10 C18:1. Maintenance of MMP below 39–40 °C suggests that the mammary gland was able to secrete only milk fat with adequate fluidity and that MFD could be an adaptation mechanism to prevent secretion of milk with higher MMP. 相似文献
12.
Developmental changes in the immune‐related cells of the gut during the first 2 weeks post hatching were determined in broiler chickens fed a corn–soybean meal (CS) based diet, a semi‐purified diet with high fat (HF) and a high carbohydrate (HC) diet. The expression of CD3 and interferon‐gamma (IFN‐γ) mRNA increased during 3–7 days of age. When the chicks were fed the CS diet, the expression of interleukin‐2 mRNA in the foregut did not increase with age. The expression of iNOS mRNA was lowered at 3 days of age in the fore‐ and mid gut but the expression was constant at the other days of age. TLR‐2 mRNA expression increased in three section of the gut during 3–7 days of age, while TLR‐4 expression increased during 0–3 days of age. Chicks fed the HC diet showed higher interleukin‐2 but lower IFN‐γ mRNA expression than birds fed the CS diet. Feeding the HF diet tended to decrease the expressions determined. These findings suggest that the functional maturation of the T cell does not always correlate with an increase in T cell numbers, maturation of innate immunity may occur earlier than that of T cells and possibly the dietary composition modifies the developmental pattern. 相似文献
13.
为了研究地塞米松、油酸、乳酸对HSL mRNA表达的影响,试验采用实时荧光定量方法检测脂肪细胞中HSL mRNA的表达量。结果表明:油酸、乳酸抑制脂肪细胞内HSL mRNA表达,抑制作用随着浓度增加而增强;地塞米松促进脂肪细胞内HSL mRNA表达,促进作用存在剂量依赖性。 相似文献
15.
选择自然发生的酮病奶牛、脂肪肝奶牛和正常围产期荷斯坦奶牛(对照)各5头,通过手术方法采取其肝脏样品,采用半定量RT—PCR方法检测了这3组奶牛肝胜样品胰岛素受体(InsR)mRNA丰度的变化。结果表明,酮痛奶牛InsRmRNA相对表达量低于正常对照奶牛,而高于脂肪肝奶牛;脂肪肝奶牛InsR mRNA相对表达量低于正常对照奶牛和酮病奶牛(P〈0.05)。可见,酮病奶牛InsR mRNA相对表达量下降,提示此有利于酮病奶牛糖异生和脂肪动员,从而缓解能量负平衡;脂肪肝奶牛InsR mRNA相对表达量降低,提示奶牛胰岛素应答显著减弱,此时奶牛可能发生了胰岛素抵抗。 相似文献
16.
The purpose of this study is to elucidate developmental changes in muscle fiber type in the pig during pre‐ and postnatal development. For this purpose, we performed a histochemical analysis for myosin adenosine triphosphatase activity to assess muscle fiber type and determined abundances of messenger RNA (mRNA) of myosin heavy chain (MHC) isoforms. Samples of Longissimus dorsi (LD) muscle were taken from fetuses on day 90 of the fetal stage. Further, samples of LD, Rhomboideus and Biceps femoris (B. femoris) muscles were taken from pigs when they were 1, 12, 26, 45 or 75 days old. Expression of MHC 2b mRNA in the LD and the B. femoris muscles rapidly and considerably increased from the late fetal stage to the early postnatal stage and this increase was associated with the development of type 2b fibers at least in the LD muscle. As shown by the rapid and considerable changes in expression of MHC 2b mRNA, it seems that a certain plasticity of muscle fiber type still remains in this developmental stage. 相似文献
17.
The aim of this study was to investigate the phenotypic relationship between feed intake (FI) characteristics with intramuscular fat, cholesterol and fatty acid composition in pork. Data were available on 202 Duroc barrows. Intramuscular fatness was positively correlated with a higher percentage of saturated (SFA) and monounsaturated fatty acids (MUFA), a lower percentage of polyunsaturated (PUFA), omega-3 (ω3) and omega-6 (ω6) fatty acids, and a lower ω6/ω3 and P:S ratio (p < 0.05). Faster growth resulted in a higher level of SFA and a lower level of ω3 fatty acids (p < 0.05). Increased FI, residual feed intake (RFI) and feed intake time (TIME) and a decreased feed intake frequency (FREQ) resulted in an increase in MUFA, a decrease in PUFA, ω3 and ω6 fatty acids and a lower P:S ratio (p < 0.05). In addition, increased FI and RFI and a decreased FREQ resulted in an increase in SFA (p < 0.05). Most of the correlations were a result of a concomitant relationship with intramuscular fatness and disappeared after correction for that trait. Serum total, high-density lipoprotein and low-density lipoprotein cholesterol levels were positively correlated with SFA and MUFA, and negatively with PUFA, omega-3 and omega-6 fatty acids, and the P:S ratio (p < 0.05). These results have implications for programmes aimed at improving meat quality through selection. 相似文献
18.
The effects of dietary fat supplementation on performance, fatty acid (FA) composition of tissues and antioxidant defence system of broilers were studied. Male broilers were placed in 20 floor pens (60 broilers per pen). The broilers were fed by diets with added different energy sources: lard (L); sunflower oil (SFO); soybean oil (SBO); and linseed oil (LSO). The treatments did not modify significantly growth performance and feed intake of the broilers. There was no effect of dietary FA pattern on reduced glutathione level and glutathione peroxidase activity of plasma, erythrocyte and liver samples. However, higher PUFA content of the diet resulted in a significant increase in malondialdehyde level of erythrocytes and liver. The broilers fed LSO diet more effectively maintained their antioxidant status with enhanced plasma radical scavenger capacity. FA composition in tissues reflected the FA pattern of the diets, although proportion of FAs with four or more double bonds was metabolic specific. LSO diet increased the level of C18:3, C20:5 and C22:6 in tissue lipids in relation to L, SFO and SBO diets. Significantly increased plasma radical scavenging capacity in concert with the enhanced C20:5 and C22:6 proportion in liver and muscle during LSO feeding indicate metabolic changes to counteract the oxidative injury. This may be related to the compounds produced after different biochemical pathways of n-6 and n-3 FAs. 相似文献
19.
Sterol regulatory element-binding factor 1 ( SREBP1) plays an important role in the lipogenesis which affects fatty acid (FA) composition in backfat and consequently influences beef nutritional quality. This study analyzed the association of 84 bp-indel, both short (S) and long (L) alleles in intron 5 of SREBP1, with FA composition and gene expression of SREBP1 in backfat of northern Spanish beef breeds (Pirenaica, Salers and Holstein-Friesian). Phylogenetic analysis suggests that 84 bp-indel of ruminants is a highly conserved region compared with those in the full-length sequence of intron 5 or mRNA of SREBP1 among species. Overall, higher content of polyunsaturated FAs was observed in SL genotype compared to LL genotype of 84 bp-Indel ( p < .05). In particular, in Pirenaica, SL genotype was associated with a higher content of stearic (18:0), α-linolenic (18:3n-3) acid, and total n-3 content ( p < .05). However, the gene expression of SREBP1 did not differ among genotypes of 84 bp-Indel ( p > .05). 相似文献
20.
The aim of this study is to examine 1) muscle fiber type composition, 2) myofiber diameter, and 3) aquaporin (AQP) 7 and AQP 9 mRNA expressions by quantitative PCR in muscles of obese db/db mice. The myofiber type composition of skeletal muscle was not statistically significantly different between db/db mice and control mice; while the average myofiber diameter ratio showed a decrease in db/db mice. The expression of AQP7 but not AQP9 mRNA in the skeletal and cardiac muscles was significantly upregulated in db/db mice. Thus this study revealed quantitatively that type 2 myofiber atrophy was shown in the skeletal muscles of db/db mice. AQP7 mRNA expression was upregulated in the skeletal and cardiac muscles of db/db mice. 相似文献
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