共查询到1条相似文献,搜索用时 0 毫秒
1.
Effect of porcine glucagon‐like peptides‐2 on tight junction in GLP‐2R + IPEC‐J2 cell through the PI3k/Akt/mTOR/p70S6K signalling pathway
下载免费PDF全文
![点击此处可从《Journal of animal physiology and animal nutrition》网站下载免费的PDF全文](/ch/ext_images/free.gif)
K. K. Qi Y. Q. Sun J. Wan B. Deng X. M. Men J. Wu Z. W. Xu 《Journal of animal physiology and animal nutrition》2017,101(6):1242-1248
Because of rare glucagon‐like peptide‐2 (GLP‐2) receptor (+) cells within the gut mucosa, the molecular mechanisms transducing the diverse actions of GLP‐2 remain largely obscure. This research identified the naturally occurring intestinal cell lines that endogenously express GLP‐2R and determined the molecular mechanisms of the protective effects of GLP‐2‐mediated tight junctions (TJ) in GLP‐2R (+) cell line. (i) Immunohistochemistry results showed that GLP‐2R is localised to the epithelia, laminae propriae and muscle layers of the small and large bowels of newborn piglets. (ii) GLP‐2R expression was apparent in the cytoplasm of endocrine cells in IPEC‐J2 cell lines. (iii) The protein expressions of ZO‐1, claudin‐1, occludin, p‐PI3K, p‐Akt, p‐mTOR and p‐p70S6K significantly (p < 0.05) increased in GLP‐2‐treated IPEC‐J2 cells, and all of them significantly (p < 0.05) decreased when LY‐294002 or rapamycin was added. GLP‐2 improves intestinal TJ expression of GLP‐2R (+) cells through the PI3k/Akt/mTOR/p70S6K signalling pathway. 相似文献