Effect of Nutrient Application on Growth,Metabolic and Enzymatic Activities of Rice Seedlings During Flooding Stress and Subsequent Re‐Aeration

The impact of submergence on the allometry, changes in metabolic activities and antioxidant enzymes during oxidative stress in four Indica rice varieties namely IR‐20, IR‐64 Sub1, Swarna Sub1 and Savitri Sub1 was studied. The differential response of flooding under clear and turbid water with different nutrient application schedules was also examined during and after 12 days of complete submergence. Submergence substantially reduced allometric parameters and the activities of antioxidant enzymes but increased the % change in chlorophyll, soluble sugars and malondialdehyde (MDA) across cultivars with drastic effects on IR‐20. Turbid water resulted in higher leaf senescence, lodging, higher depletion of chlorophyll and soluble sugars because of poor light transmission. Pre‐submergence N application resulted in higher lodging, leaf senescence and higher MDA level but depletion of chlorophyll and NSC leading to higher % change over prior to submergence. Basal P application reduced the senescence and lodging, whereas increased the antioxidant enzyme activities. Foliar spray of post‐submergence N with basal P improved the retention and regain of chlorophyll, soluble sugar and increased the dry matter, leaf area and root shoot ratio. Crop establishment could therefore be enhanced in areas where untimely flooding is anticipated by applying basal P and foliar spray of urea after desubmergence.     

1,2,4-三氯苯胁迫对水稻分蘖盛期植株生长和生理特性的影响

在土培条件下,以对1,2,4-三氯苯(TCB)敏感性显著差异的宁粳1号(敏感)和扬辐粳8号(耐性)为对象,研究了不同浓度1,2,4-三氯苯(TCB)胁迫对两种水稻分蘖盛期生长和生理特性的影响。结果表明,不同浓度TCB胁迫下,敏感基因型宁粳1号最长根长、株高、单穴分蘖数、地上部和地下部干物重随胁迫程度加深均显著递减; 耐性基因型扬辐粳8号在低浓度(20 mg kg^-1) TCB胁迫下,最长根长、地上部和地下部干物重极显著增加,当中高浓度(40 mg kg^-1、60 mg kg^-1) TCB胁迫时,最长根长、株高、单穴分蘖数、地上部和地下部干物重显著下降。不同浓度TCB胁迫下,敏感基因型宁粳1号根系活力、叶绿素含量、叶片和根系可溶性蛋白质含量相对较低,叶片和根系超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性明显降低,O₂^?产生速率和丙二醛(MDA)含量显著增高; 耐性基因型扬辐粳8号在低浓度(20 mg kg^-1) TCB胁迫下,根系活力、叶片和根系的可溶性蛋白含量、SOD、POD、CAT活性极显著增加,O₂^?产生速率和MDA含量极显著降低,当中等浓度(40 mg kg^-1) TCB胁迫时,SOD、POD、CAT活性虽然增强,但O₂^?产生速率显著增加,MDA大量积累,当高浓度(60 mg kg^-1) TCB胁迫时,其根系活力、叶绿素含量极显著降低,根系中SOD、CAT活性显著降低,O₂^?产生速率和MDA含量显著增加。总之,低浓度TCB胁迫下,长势良好、叶片和根系可溶性蛋白质含量高、抗氧化系统清除活性氧能力强、膜脂过氧化程度低是耐性基因型扬辐粳8号区别于敏感型宁粳1号的主要特征。     

Salinity tolerance of wild barley Hordeum vulgare ssp. spontaneum

Salinity tolerance of 47 wild barley genotypes and six barley cultivars was evaluated under control and salinity stress (300 mM NaCl) conditions. Shoot and root dry weight (DW), plant height, membrane stability index (MSI), relative water content, survival rate, leaf malondialdehyde (MDA) and proline contents, root and leaf Na, K, Ca and K/Na ratio, and chlorophyll a fluorescence were measured. Salinity stress caused significant increase in the MDA, proline content, Na and Ca concentrations of the roots and leaves, but resulted in a decrease in the other traits. H. spontaneum genotypes were considerably less affected by the salinity than the genotypes of H. vulgare. Plant survivability was negatively correlated with the Na concentration (r =−.66) but positively correlated with the leaf K/Na ratio (r = .67) and MSI (r = .68). Tolerance mechanisms such as ion exclusion (Na) were likely to be present in the wild barley causing K/Na homeostasis as well as the much lower root and shoot Na, resulting in the higher survival rate.     

苗期耐低氮基因型苦荞的筛选及其评价指标

土壤缺氮是造成我国黄土高原冷凉地区农业低产的主要原因。为了筛选耐低氮苦荞基因型及适宜鉴定指标,采用苗期水培方式,测定9个不同基因型苦荞在不同氮水平下的农艺性状、生理特性及植株氮素利用等指标,采用隶属函数法计算各指标耐低氮指数,通过主成分分析与聚类分析评价各基因型苦荞综合耐低氮能力。结果表明,低氮胁迫下,苦荞地上部生长受抑制程度大于根系,株高、茎粗、叶面积、地上部干重、根系体积、根系表面积及根系平均直径均呈下降趋势,主根长和根冠比呈上升趋势;根系活力、根系硝酸还原酶(NR)活性及可溶性蛋白含量有所下降,而根系超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、丙二醛(MDA)、可溶性糖及游离脯氨酸含量有所升高;叶片叶绿素含量、Fm和Fv/Fm降低,而Fo升高;植株全氮含量及氮积累量降低,而由于植株氮积累量降幅明显大于生物量降幅,导致氮利用效率显著升高。将25个单项指标转化为4个相互独立的综合指标(累计贡献率达87.44%),并将9个苦荞基因型划分为耐低氮型、中间型和不耐低氮型。为了预测苦荞基因型的耐低氮能力,将D值作因变量,各指标耐低氮指数作自变量进行逐步回归分析,建立最优回归方程,筛选出株高、茎粗、叶面积、根冠比、叶绿素含量、Fm、根系超氧化物歧化酶(SOD)活性及氮利用效率8项对苦荞耐低氮能力有显著影响的指标,并且9个苦荞基因型的苗期耐低氮能力预测值Y与D值显著相关(R2=0.998,P0.01),表明这8个指标可用于耐低氮苦荞基因型的快速鉴定。     

甘蓝型油菜氮素高效吸收的植株形态与生理特性研究

为阐明甘蓝型油菜氮素高效吸收的形态和生理机制,利用6个氮素效率差异显著的甘蓝型油菜为供试材料,分析低氮条件下甘蓝型油菜抽薹期地上部和根系形态、叶片和根系生理特性的基因型差异及这些指标与高效氮素吸收的关系。结果表明,3个氮高效基因型在表型性状（株高、叶片数、最大叶长×宽、地上部干重、茎基粗、根长、根表面积、根体积、根平均直径和根干重）、生理（叶片可溶性糖含量、游离氨基酸总量、根系吸收总面积、活跃吸收面积、游离氨基酸总量和根系活力）和光合方面（净光合速率、气孔导度、胞间CO₂浓度、蒸腾速率和氮素光合效率）均高于3个氮低效基因型。甘蓝型油菜抽薹期根平均直径、最大叶长×宽、蒸腾速率和根系硝酸还原酶共同决定了氮素吸收效率的92.10%。     

铁营养对烟草幼苗生长及生理生化指标的影响

采用水培试验方法,研究了不同铁营养水平下（0.00、0.56、1.12、1.68、3.36、4.48 mg/L）,烟草幼苗生长及其外渗电导率、可溶性糖含量、可溶性蛋白含量、游离脯氨酸含量、丙二醛含量和根系活力的关系。结果表明：铁浓度为1.68mg/L处理后的烟草幼苗生长状况较好,株高,根冠比均明显好于其它铁离子处理;抗性生理指标外渗电导率、游离脯氨酸含量及丙二醛含量均低于供试的其它铁离子处理,而可溶性糖、可溶性蛋白含量及根系活力均高于供试的其它铁离子处理。     

Elevated CO2 increases wheat cer, leaf and tiller development, and shoot and root growth

Whole-plant responses to elevated CO₂ throughout the life cycle are needed to understand future impacts of elevated atmospheric CO₂. In this study, Triticum aestivum L. leaf carbon exchange rates (CER) and carbohydrates, growth, and development were examined at the tillering, booting, and grain-filling stages in growth chambers with CO₂ concentrations of 350 (ambient) or 700 (high) μmol mol^?1. Single-leaf CER values measured on plants grown at high CO₂ were 50% greater than those measured on plants grown at ambient CO₂ for all growth stages, with no photosynthetic acclimation observed at high CO₂. Leaves grown in high CO₂ had more starch and simple sugars at tillering and booting, and more starch at grain-filling, than those grown in ambient CO₂. CER and carbohydrate levels were positively correlated with leaf appearance rates and tillering (especially third-, fourth- and fifth-order tillers). Elevated CO₂ slightly delayed tiller appearance, but accelerated tiller development after appearance. Although high CO₂ increased leaf appearance rates, final leaf number/culm was not effected because growth stages were reached slightly sooner. Greater plant biomass was related to greater tillering. Doubling CO₂ significantly increased both shoot and root dry weight, but decreased the shoot to root ratio. High CO₂ plants had more spikes plant^?1 and spikelets spike^?1, but a similar number of fertile spikelets spike^?1. Elevated CO₂ resulted in greater shoot, root and spike production and quicker canopy development by increasing leaf and tiller appearance rates and phenology.     

辽宁苗期玉米根叶对水分胁迫的响应

为了提炼辽宁春玉米苗期干旱胁迫影响的定量化评价指标,以‘丹玉39’为试验材料,采用盆栽称重控水法,对不同水平土壤含水量对春玉米苗期根叶生长的影响进行研究。结果表明：正常（75%）和偏湿（85%）水分条件下玉米具有较高的株高,茎粗和叶面积。干旱胁迫下,玉米生长发育受到抑制,株高降低、茎粗变细、根系和地上部的生物量降低,叶片光合面积减小,根冠比增大。但一定程度的干旱（55%）有利于根系下扎,增大根冠比。干旱胁迫对玉米的株高和根系的生长发育有较大的影响,地上生物量受干旱胁迫影响大于根系。     

根区温度对黄瓜幼苗生长和光合特性的影响

试验对日光温室黄瓜幼苗进行了不同根区温度处理。结果表明,与适温处理(ck)相比,亚适温和低温处理降低了黄瓜幼苗的株高、茎粗、叶面积、地上部干重和全株干重,而增加了地下部干重和根冠比,降低了黄瓜幼苗叶片叶绿素含量、光合速率、蒸腾速率、气孔导度和胞间CO2浓度。     

品种和外植体对马铃薯脱毒试管苗生长的影响

采用二因素随机区组试验设计,通过测定苗鲜重、根鲜重、苗干重、根干重、叶片数、株高、茎粗、根长、根条数、节长、节数和叶面积等生长指标,研究了品种和外植体对马铃薯脱毒试管苗生物量,地上部和地下部的影响。研究结果表明:3个不同成熟期品种、3种不同外植体部位对马铃薯单株根鲜重、苗干重、根干重、株高、茎粗、节长和根冠比影响有显著性交互作用(p<0.05),说明不同品种和外植体对马铃薯脱毒试管苗生长发育有直接影响,其余性状受品种和外植体交互作用影响不显著;3个不同成熟期品种马铃薯的苗鲜重、根鲜重、苗干重、根干重、叶片数、茎粗、节长、节数、根冠比和根条数受品种影响显著(p<0.05),其余性状受品种影响不显著;3种不同外植体处理对马铃薯的根鲜重、苗干重、根干重、株高、茎粗、节数、叶面积和根冠比有显著性影响(p<0.05),其余性状受3种外植体的影响不显著。其中,以一个节段转接最有利于马铃薯脱毒试管苗的生长。     

油菜素甾醇(BRs)对杂交粳稻灌浆期光合物质、群体氮素积累和利用的影响

以常规杂交粳稻常优3号和籼粳杂交超级稻甬优12为材料,通过叶面施肥分析了油菜素甾醇(BRs)对2个杂交粳稻灌浆期剑叶光合特性、群体干物质积累、氮素吸收利用、非结构性碳水化合物(NSC)积累和植株不同器官NSC/N的影响。结果表明,BRs对2个水稻叶片光合生理指标的影响表现为G_s>T_r>P_n>C_i。喷施2,4-表油菜素内酯(eBL)后能够增强灌浆期叶片净光合速率和蒸腾速率,维持叶绿素含量和胞间CO₂浓度,从而提升叶片光合能力;以及增加群体干物质、氮素和NSC积累量以及干物质积累比例和积累速率、氮素吸收比例和吸收速率、NSC转运比例和转运速率,但降低了氮素干物质生产效率;喷施油菜素吡咯(Brz)后的上述生理效应相反。eBL提升了2个水稻品种花后干物质、NSC和氮素的转移量及氮素对籽粒贡献率,降低了干物质和NSC对籽粒贡献率;Brz则降低了干物质、NSC及氮素的转移量和转移率,提升了干物质和NSC对籽粒贡献率。2个杂交粳稻整个灌浆期地上部不同器官NSC、氮素转运量均表现为eBL>CK>Brz,籽粒灌浆前期>籽粒灌浆后期。2个水稻品种地上部不同器官NSC/N随着籽粒的不断充实呈现上升趋势,其中茎鞘、穗部NSC/N变化最为显著;eBL减小了叶片和茎鞘中的NSC/N,Brz则反之,但喷施BRs后穗部变化规律不明显。籼粳杂交超级稻甬优12比常规杂交粳稻常优3号具有更强的剑叶光合速率,更高的干物质和氮素及NSC积累量,更大的灌浆前期NSC和氮素转运量,最终籽粒实际产量较常优3号增加5.03%~9.32%;eBL对2个水稻品种干物质和氮素积累及NSC转运作用规律不一,Brz对甬优12干物质、氮素积累及NSC转运抑制效应大于常优3号。     

相互竞争对栽培稻和杂草稻形态特性的影响

采用盆栽试验研究杂草稻和栽培稻相互竞争对各自形态特征的影响,杂草稻和栽培稻按照2:1和1:2的比例各自插植在同一盆中,以单独插植杂草稻和栽培稻的处理为对照,分别测定他们的分蘖数、株高、叶片长宽、干物质积累和根系性状。结果显示：杂草稻与栽培稻的竞争对栽培稻‘沈农265’的株高、叶片形态影响不大,使分蘖数、干物质积累和根系性状显著降低;杂草稻与栽培稻的竞争对杂草稻‘WR04-12’的分蘖数、株高、叶片形态、干物质积累和根系性状都略有增加,但没有达到显著水平;另外,在同一生育时期,杂草稻‘WR04-12’的分蘖数、株高和根系性状都比栽培稻‘沈农265’高,处于竞争中的优势地位,而栽培稻受杂草稻的遮挡,处于劣势地位。因此,杂草稻和栽培稻相互竞争限制了栽培稻‘沈农265’的生长和干物质的积累,造成栽培稻‘沈农265’减产。     

Mechanisms of resistance to shoot fly, <Emphasis Type="Italic">Atherigona soccata</Emphasis> in sorghum

Summary Sorghum shoot fly, Atherigona soccata (Rondani) is an important pest of sorghum in Asia, Africa, and Mediterranean Europe, and host plant resistance is an important component for the management of this pest. The levels of resistance in the cultivated germplasm are low to moderate, and therefore, it is important to identify genotypes with different mechanisms of resistance to pyramid the resistance genes. We studied the antixenosis for oviposition, antibiosis, and tolerance components of resistance in a diverse array of shoot fly-resistant and -susceptible genotypes. The main plants and tillers of SFCR 151, ICSV 705, SFCR 125, and, IS 18551 experienced lower shoot fly deadhearts at 28 days after seedling emergence, produced more number of productive tillers. The insects fed on these genotypes also exhibited longer larval period (10.1–11.0 days compared to 9.3 days on Swarna), lower larval survival and adult emergence (54.7–67.8 and 46.7–52.2% compared to 73.3 and 60.6% on Swarna, respectively), and lower growth and adult emergence indices as compared to the susceptible check, Swarna. Physico-chemical traits such as leaf glossiness, trichome density, and plumule and leaf sheath pigmentation were found to be associated with resistance, and chlorophyll content, leaf surface wetness, seedling vigor, and waxy bloom with susceptibility to shoot fly and explained 88.5% of the total variation in deadhearts. Step-wise regression indicated that 90.4% of the total variation in deadhearts was due to leaf glossiness and trichome density. The direct and indirect effects, correlation coefficients, multiple and step-wise regression analysis suggested that deadhearts, plants with eggs, leaf glossiness, trichomes on the abaxial surface of the leaf, and leaf sheath pigmentation can be used as marker traits to select for resistance to shoot fly, A. soccata in sorghum.     

打顶后NAA处理对烟草生长、烟叶中钾和烟碱浓度的影响

在云南省田间连续三年研究了打顶后在茎断面涂抹萘乙酸（NAA）处理对烤烟植株生长、钾素吸收、烟叶中钾和烟碱浓度的影响。结果表明,打顶导致整个烟株中钾素的钾素损失,烟叶中钾浓度下降,烟碱浓度增加。打顶后NAA处理可以增加烟株对钾素的吸收,并促进钾素向叶片中分配,使叶片钾浓度提高;同时降低叶片烟碱浓度。第一次NAA处理两周后用NAA再处理一次虽然可以进一步提高烟叶钾浓度,但使叶片干重稍有下降,且在操作上多一道工序。若开花后不打顶,虽然叶片中钾浓度较高,但大大降低了叶片干物重,严重影响叶片的产量和品质。     

水分胁迫对花生不同器官非结构性碳水化合物含量的影响

为探讨不同生育时期水分胁迫对花生不同器官非结构性碳水化合物(NSC)“库—源—流”间的变化动态,以‘花育20号’和‘花育27号’花生品种为试材,采用控制条件下的防雨棚池栽方法,研究了花生生长发育过程中不同生育时期水分胁迫下,非结构性碳水化合物(NSC)在花生叶片、茎、根和荚果等器官中的动态变化。结果表明,全生育期干旱胁迫使花生叶片、茎和根中可溶性总糖含量和淀粉均明显升高,但荚果中可溶性糖含量却明显降低。无论何生育时期受到干旱胁迫均使得叶片中可溶性糖含量峰值提前15天左右出现,“源”物质输出提前但输出量降低,叶片提前衰老。生育前期干旱胁迫使滞留在荚果中的NSC含量增加,结荚期后干旱胁迫反而利于荚果中NSC的转化。全生育期水分适宜处理叶片中NSC含量相对较低且变化较小。由此表明,干旱胁迫降低了NSC由源至库的运输和转化,使荚果“库”容量降低。     

品种基因型和温度对马铃薯脱毒试管苗农艺性状的影响

以大西洋、克新1号、青薯168脱毒基础苗为试验材料,采用二因素随机区组设计,研究不同基因型品种、不同温度对马铃薯脱毒基础苗苗鲜重、根鲜重、苗干重、根干重、叶片数、株高、茎粗、根长和根条数影响,培养14d时,测定各性状指标.结果表明,3个不同成熟期基因型品种、3种不同培养温度处理对马铃薯单株平均根鲜重、叶片数、株高影响有显著性交互作用(p＜0.05),其余性状受品种基因型和温度交互作用影响不显著.不同基因型品种对苗干重、根干重、叶片数、根条数农艺性状指标影响显著(p＜0.05),其余性状指标未达显著影响;不同培养温度对马铃薯单株平均苗鲜重、根鲜重、苗干重、根干重、茎粗、根长、根条数(p＜0.05)农艺性状指标有显著性影响(p＜0.01),其余性状指标未达显著影响;3个不同基因型品种所需的适宜培养温度为(23±2)℃,此培养温度时,3个品种基因型的农艺性状表现最好.     

壮秧剂对寒地超级稻龙稻5号秧苗素质及产量提高的作用

以寒地超级稻龙稻5号为供试品种,在寒地早春大棚旱育秧条件下,研究了不同壮秧剂及用量对水稻秧苗素质及本田产量构成的影响,旨在探讨适龄、健壮、适合机插秧苗群体在苗期的调控措施,为黑龙江地区培育高质量的秧苗提供理论依据和技术指导。结果表明：施用壮秧剂处理秧苗地上部鲜、干重,株高,叶片最大长度、宽度及面积,叶挺长、叶龄,苗基宽,本田最终分蘖和穗结实率均显著大于对照处理（不使用壮秧剂）。研究结果还表明,壮秧剂施入后增产的主要原因是显著提高了穗数和二次枝梗结实率。此外,处理3（育苗伴侣每育秧钵盘使用12g,用机插秧专用拌种剂旱育保姆包衣水稻种子后,直接播种）、处理 4（育苗伴侣每育秧钵盘使用12g,用机插秧专用拌种剂旱育保姆包衣水稻种子后,浸种催芽播种）秧苗根干重、根冠比均显著大于其他处理（根冠比CK除外）;处理5（三金壮秧剂当地用量,每育秧钵盘使用41.6g,水稻种子浸种催芽播种）叶长和株高显著大于其他处理,但其充实度小于处理1（育苗伴侣每盘使用15g,水稻种子浸种催芽播种）、处理2（育苗伴侣每盘使用12g,水稻种子浸种催芽播种）、处理3、处理4,且与处理3、处理4差异显著。     

Pattern of genetic inheritance of morphological and agronomic traits of sorghum associated with resistance to sorghum shoot fly, <Emphasis Type="Italic">Atherigona soccata</Emphasis>

Sorghum shoot fly, Atherigona soccata is an important pest of sorghum during the seedling stage, which influences both fodder and grain yield. To understand the nature of inheritance of shoot fly resistance in sorghum, we performed generation mean analysis using two crosses IS 18551 × Swarna and M 35-1 × ICSV 700 during the 2013–2014 cropping seasons. The F₁, F₂, BC₁ and BC₂ progenies, along with the parental lines were evaluated for agronomic and morphological traits associated with resistance/susceptibility to sorghum shoot fly, A. soccata. The cross IS 18551 × Swarna exhibited significant differences between the parents for shoot fly deadhearts (%) in the postrainy season. The progenies of this cross exhibited lower shoot fly damage, suggesting that at least one of the parents should have genes for resistance to develop shoot fly-resistant hybrids. Leaf glossiness, leafsheath pigmentation and plant vigor score during the seedling stage exhibited non-allelic gene interactions with dominant gene action, whereas 100 seed weight showed both additive and dominant gene interactions. Presence of awns showed recessive nature of the awned gene. Generation mean analysis suggested that both additive and dominance gene effects were important for most of the traits evaluated in this study, but dominance had a more pronounced effect.     

水稻分蘖芽的环境敏感期研究

本文以早籼稻、晚粳稻、籼型杂交稻等5个具有不同分蘖力的水稻品种为材料，研究了水稻分蘖芽分化发育过程中对外界环境的敏感期。结果表明：水稻分蘖芽从分蘖原基形成起，经过一幼一基、二幼一基、三幼一基、四幼一基期后、其第一叶就超过前出叶。然后伸出母茎叶鞘，长成正常分蘖。在这个过程中，三幼一基向四幼一基发展的转     

半干旱地区菊芋品系植株表型与光合特性分析

为了加快半干旱地区的新型资源作物菊芋品种的选育进程,探讨菊芋产量及相关性状表型和光合生理基础是必要的。选用本课题组收集和培育的4个品系( 2个高产,2个低产品系),设置随机区组试验,比较菊芋高产和低产品系与产量形成相关的生物量累积、植株表型和光合特性的差异。结果表明,高产菊芋品系的地上生物量、块茎产量及根生物量显著高于低产品系;高产品系整体表现出明显的生长优势,其株高、基径、叶面积和叶片数均显著高于低产品系。气体交换参数分析表明,高产品系的光补偿点和暗呼吸速率显著小于低产品系,而其他各项参数差异不显著。相关性分析表明,菊芋块茎产量与株高、基径、叶面积、叶片数、地上生物量和根生物量呈显著正相关,而与光补偿点和暗呼吸速率呈显著负相关。建议多的叶片数、大的叶面积和发达的根系作为半干旱地区菊芋高产品系田间选育指标,而低的光补偿点和暗呼吸速率在高产品系选育中也具参考价值。