Integrated Control Technique of Poplar Diseases in China

IntroductionNowthereservedarea-ofartificialforestsis3.3333xlO7hm2inChina,whiletheareaofPoplaris6.6xlo6hm2.ltmakesChinabecomethelargestcountryinpossess-ingartificialforestsofPoplar.PoplarvarietiesandnumberincreasegraduallyWithartificialforestsofpopIarrapid…     

Hybrid poplar (Populus spp.) adaptation to a semi-arid region: results from Northwest New Mexico (2002–2011)

A provenance test was initiated in spring 2002 at the New Mexico State University Agricultural Science Center at Farmington to examine the adaptability of various hybrid poplar (Populus spp.) crosses to the high elevation, semi-arid conditions of this region of the Southwestern United States. Ten crosses of P. deltoides, P. maximowiczii, P. nigra and P. trichocarpa obtained from nurseries in the Pacific Northwest were grown in replicates (3 plots × 16 trees) under drip irrigation programmed to match tree evapotranspiration (ET) rates. By the end of year 10, six crosses had maintained a 90 % or higher survival rate and had an average wood volume of 246 m³ ha^?1. The P. deltoides × P. nigra (P. × canadensis) clone OP-367 surpassed a ten-year commercial target of 25-cm diameter at breast height (DBH) after eight seasons, and by the end of 2011 attained a DBH of 28.0 cm, height of 19.9 m and wood volume of 473 m³ ha^?1. Results suggest that hybrid poplar production is possible in this type of semi-arid environment using appropriate germplasm and drip irrigation regimes scheduled according to tree ET demand.     

Detection of phytoplasma infections in declining Populus nigra ‘Italica’ trees and molecular differentiation of the aster yellows phytoplasmas identified in various Populus species

A disease of Populus nigra‘Italica’ associated with foliar yellowing, sparse foliage, stunting, dieback, and decline was observed in south-western Germany; a witches’ broom disease of Populus alba that is known in other countries was also detected in Hungary and Germany. The aetiology of the diseases was studied by fluorescence microscopy and polymerase chain reaction (PCR) amplification. Using fluorescence microscopy, phytoplasmas could be detected only in P. alba. However, most diseased trees of P. nigra‘Italica’ tested phytoplasma-positive by PCR. In some of the trees the phytoplasma numbers were so low that nested PCR was required to detect the infection. Very low phytoplasma numbers were also observed in diseased Populus tremula. The identity of phytoplasmas from P. nigra‘Italica’ sampled in Germany and France, P. alba and also P. tremula was examined by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified ribosomal DNA. In all poplars, phytoplasmas of the aster yellows group were detected. However, three different RFLP groups were identified that consisted of (1) French strains from P. nigra‘Italica’, (2) German strains from P. nigra‘Italica’ and (3) strains from P. alba and P. tremula. The profile observed in the last group was probably the result of sequence heterogeneity in the two 16S RNA genes.     

Effect of some chemicals employed in the detached leaf culture of Populus on the infection of Melampsora larici-populina

A study was made on the effect of certain chemicals and their combinations on the longevity of the leaf disks cut from Populus × euramericana‘1-488’and P. alba cv. hickeliana. Benzimidazole and gibberellic acid maintained (up to a maximum of 80 days) the leaf disks better than the others, however, the longevity was dependent on the chemical and the concentration employed. In a factorial experiment of 3 chemicals × 2 concentrations × 2 cultivars × 2 races of the fungus, it was found that. all these factors contributed significantly (P<0.05) to the experimental variance. However, differential effects of the chemicals or the concentrations on the cultivar × race interaction were not observed.     

THE BEHAVIOR OF ADULT OF OSIER WEEVIL

THEACTIONLAWoFADULTThcadultsofOsierweevilwereobservedonafixedspotinfieldandraisedindoorsandinfieldseparatelyinl987-9o.Theboxes(4()x4()x6ocm)ineachofWhichl5pairsofadultswereplacedwereusedwiththrcere-peats.Thesamesizesofboxesinwhichtl1eadultsxvcrcraisedx`eredesignedindoorswithtWorepeats.l5canswerealsousedineachofwhlchapairofadultwasplaced.ThefreshbranchesofPoplarthattheadultswcrelikctoeatt`crereplacedilladayabout.ThcEmcrgcnccLawofAdultOsierwecvilstartcdemergenceinthclasttendaysofJuly.…     

Effects of Thidiazuron,basal medium and light quality on adventitious shoot regeneration from <Emphasis Type="Italic">in vitro</Emphasis> cultured stem of <Emphasis Type="Italic">Populus alba</Emphasis>×<Emphasis Type="Italic">P. berolinensis</Emphasis>

The effect of Thidiazuron （TDZ）, basal media and light quality on adventitious shoot regeneration from in vitro cultured stem of Populus albaxP berolinensis were determined to establish a high efficiency shoot regeneration system from stem explants of P. alba~P berolinensis. Stems ofPopulus alba~P berolinensis were collected from cultured shoots in vitro derived from dormancy buds of 3-year-old seedlings. The stem explants were cultured on MS medium containing 0.02-mg·L-1NAA （naphthaleneacetic acid）, and 0.1, 0.3, 0.5 and 1.0 mg·L-1 concentrations of TDZ to determine the effect of TDZ on shoot regeneration. Three basal media, i.e. MS, woody plant medium （WPM） and B5, were used to test their influences of different media on adventitious shoot regeneration. Green, red, blue and yellow plastic films in comparison with florescent light as control were used to observe their effects on shoot regeneration. The results showed that differ- ent concentrations of TDZ had an evident influence on shoot regeneration. Lower concentration of TDZ （0.1 mg·L-1） resulted in more ad- ventitious shoot regeneration and higher concentration of TDZ （〉0.1 mg·L-1） inhibited shoot regeneration. Among different media, MS medium exhibited a high efficiency for shoot regeneration, followed by WPM medium, while B5 medium inhibited shoot regeneration. Normal light and yellow light exhibited better effects on shoot regeneration, compared with other light.     

Interactive effects of irrigation and exponential fertilization on nutritional characteristics in <Emphasis Type="Italic">Populus</Emphasis> × <Emphasis Type="Italic">euramericana</Emphasis> cv. ‘74/76’ cuttings in an open-air nursery in Beijing,China

Nutritional characteristics determine tree stock quality to a considerable extent. Exponential fertilization can induce nutrient reserves within juvenile trees, but its validity on poplar is contingent on interaction with a scientific irrigation regime due to limited water resources under global warming. In the present study, we raised 3200 Populus × euramericana cv. ‘74/76’ cuttings under four irrigation regimes of 0 (I0), 60 % (I60), 80 % (I80), and 100 % (I100) of field capacity for soil moisture content with or without (control) the employment of nitrogen (N) addition delivered as exponential fertilization at the rate of 8 g N cutting^?1 (E8) in an open-air nursery in Beijing, China. Both height and diameter increased with the increment of soil moisture ratio or in response to exponential fertilization (EF) without any interactive effects. In general, concentrations of N, phosphorus (P), and potassium (K) declined with time in stem but foliar N concentration did not change. Under the I100 regime, EF increased foliar N concentration relative to the control but failed to affect N concentration in stem in September, when both N concentration and N content were increased by EF under the I80 regime. Stem-K content and concentration by EF under the I80 regime also increased in September, therefore EF-treated cuttings had a higher ratio of K content in stem to that in whole plant (%ANAR). Vector diagnosis for nutritional status indicated that EF resulted in dilution of K concentration but induced a steady-state P uptake in leaves under all irrigation regimes. Therefore, EF of N addition could promote N uptake to leaves of P. × euramericana cv. ‘74/76’ cuttings, but it had a null effect on N and P reserves in stem and impaired K reserves. In conclusion, the irrigation regime of 80 % field capacity of soil moisture content was suggested for the culture of juvenile P. × euramericana cv. ‘74/76’ cuttings. As the interactive meaning, EF was also suggested for its excellent promotion on both N and K uptakes.     

10~11年生杨树品系抗杨干象水平及其与树干物理特性的关系

[目的]开展不同品系的杨树亲本来源、干部物理特性与抗性关系研究,为抗杨干象新品系的选育奠定一定基础.[方法]本研究连续2年研究了10个亲本51个不同杨树品系10~11年生杨树的被害株率、杨干象虫口密度与杨树木质部硬度、树皮硬度、树皮厚度、胸径等的关系.[结果]免疫品系、高抗虫品系、抗虫品系、感虫品系和高感虫品系木质部平均硬度分别为(47.38±1.71 ) HD、(46.07±1.17) HD、(44.64±1.61) HD、(41.84±1.66) HD、(40.73±2.04) HD;树皮平均硬度分别为(40±3.53) HD、(39.99±0.96) HD、(37.63±0.46) HD、(32.35±1.54) HD、(31.7±0.52) HD;平均胸径分别为(361.64±13.8) mm、(313.8±6.19) mm、(309.98±5.27) mm、(289.56±10.73) mm、(287.67±17.49) mm,亲本为美洲黑杨×青杨、马氏杨、美洲黑杨×马氏杨、小叶杨×胡杨的品系为抗虫品系;亲本欧洲黑杨×美洲黑杨、欧洲黑杨×小叶杨、美洲黑杨的多数品系为感虫品系.[结论]杨树木质部硬度与树皮硬度越大抗杨干象能力越强;胸径生长相对较快的品系抗性强;青杨、马氏杨、小叶杨为亲本抗虫性强,美洲黑杨、欧洲黑杨为亲本抗虫性差.在抗杨干象品系育种工作中应选择青杨、小青杨或甜杨做为亲本;避免以美洲黑杨或欧洲黑杨为亲本.     

Genotypic variation in wood density and growth traits of poplar hybrids at four clonal trials

Wood density is considered as one of the most important wood properties which affects the properties and value of both fibrous and solid wood products. The present study was intended for evaluating the possibilities of improving wood quality and growth of poplar hybrids. Wood density components of individual growth rings (minimum and maximum wood density, average ring density) and growth traits (tree height, dbh, stem volume) were measured in four 10- and 12-year-old clonal trials of four poplar hybrids, Populus deltoides × P. nigra, P. trichocarpa × P. deltoides, P. maximowiczii × P. balsamifera, and P. balsamifera × P. nigra, as well as P. deltoides. Wood density components of individual growth rings were obtained from microdensitometeric profiles measured with a direct reading X-ray densitometer. Site had a moderately significant effect on wood density and a highly significant effect on tree growth. The hybrid effect was highly significant (P < 0.001) for most traits. Minimum, maximum and weighted wood densities were found to be under strong genetic control, with clonal repeatabilities varying between 0.45 and 0.81. The coefficient of genotypic variation (CV_G) for wood density at individual sites ranged from 4.0 to 6.8%, whereas CV_G for dry fiber weight (mass) reached 32.8% with repeatabilities of up to 0.67. A small but significant (P = 0.028) hybrid × environment interaction was found for dry fiber weight. The highest ecological sensitivity was found for P. deltoides × P. nigra, with ecovalence reaching 32.3%. Clonal × environment interaction was significant for weighted, average, and minimum wood density. Significant negative genotypic correlations between stem volume and wood density ranged from −0.39 to −0.74. One possible strategy in tree breeding would be to maximize wood fiber production through selection for dry fiber weight.     

Molecular characterization of a phytoplasma from the aster yellows (16SrI) group naturally infecting Populus nigra L. ‘Italica’ trees in Croatia

Leaf and branch samples were collected from 10 Populus nigra L. ‘Italica’ trees found in the Zagreb urban area. One of the P. nigra L. ‘Italica’ trees exhibited leaf yellowing, overall sparse foliage, stunting and decline. Two methods for the nucleic acid extraction in the phytoplasma detection from P. nigra were compared. A phytoplasma from the aster yellows group (16SrI) was detected by PCR in the symptomatic as well as in four apparently asymptomatic plants. The pathogens are classified, by restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene plus the spacer region, as members of a newly described subgroup 16SrI‐P. Phylogenetic analysis of 16S ribosomal and spacer region sequence confirmed their close relationship with the other members of the aster yellows group. However, RFLP analyses of other conserved genes such as tuf, BB88 and ribosomal protein rpL22 gene, clearly confirmed that this is a molecularly distinguishable phytoplasma belonging to a new ribosomal protein subgroup designated rp‐O.     

Germination of Melampsora larici-populina uredospores on poplar leaves1

Using an electron scanning microscope the germination of Melampsora larici-populina ure dospores was observed on the lower leaf surfaces of a susceptible poplar clone Populus trichocarpa and a resistant one P. בSerotina de Poitou’. The observations were made from a few hours to more then a day after placing the uredospores on the leaf surfaces.     

Interactions between Widely Spaced Young Poplars (Populus spp.) and the Understorey Environment

Silvopastoral systems involving poplars are common to rural landscapes in many parts of New Zealand. The effect of widely spaced trees of Populus nigra × P. maximowiczii, aged 8–11 years, on the surrounding micro-environment in a tree-pasture system was determined over 3 years at a southern North Island hill country site. Relative to open (unshaded, no trees) pasture, understorey pasture received 33% less radiation while radiation on the north side of trees (North) was similar to that on the south side of trees (South). Around one tree, soil temperature averaged 14.9°C annually on the North and 13.8°C on the South. Soil water content was highest in spring and winter (0.35–11;0.39 m³ m⁻³) and lowest in summer and autumn (0.21–11;0.26 m³ m⁻³), and differences occurred between plots in open pasture and those beneath trees in all seasons except spring. Soil water content of tree aspects differed slightly (<10%) in summer (South > North) and autumn (North > South), but not in spring and winter, when contents were similar. Soil pH was 0.2 units higher beneath trees than in open pasture in one of 2 years. Concentrations of Ca, K, Mg, P, and S were similar in tree and open environments. The study results complement those collected for mature trees, and will be useful in developing tree-pasture models.     

Pathogenicity of entomopathogenic fungi to <Emphasis Type="Italic">Thaumetopoea pityocampa</Emphasis> (Schiff.) (Lepidoptera: Thaumatopoeidae) larvae in laboratory conditions

Pathogenicity of 13 strains of entomopathogenic fungi belonging to genera Paecilomyces, Tolypocladium, Beauveria, Metarhizium, and Lecanicillium (=Verticillium) was evaluated on fourth instar larvae of Thaumetopoea pityocampa (Schiff.) (Lep.: Thaumatopoeidae). Larvae were treated individually by dipping into conidial suspension with the concentration of 1.0 × 10⁵ conidia ml⁻¹. All the tested isolates resulted in various levels of mortality (16–100%), and eleven of them were able to grow and sporulate on dead larvae. Mortality caused by three isolates of P. fumosoroseus, one isolate of Beauveria bassiana and one isolate of Metarhizium anisopliae were significantly higher than that of the other treatments. Three isolates of P. fumosoroseus, one isolate of P. farinosus, and one isolate of M. anisopliae showed mycelial growth and sporulation on significantly more cadavers compared to other treatments. Dose–mortality test was conducted with P. fumosoroseus (isolate no: 2679), which appeared to be the most promising isolate among the tested fungi, by spraying five concentrations of its conidia (1.0 × 10⁶, 1.5 × 10⁶, 7.5 × 10⁶, 1.0 × 10⁷ and 1.5 × 10⁷ conidia ml⁻¹). The estimation of the LC₅₀ (95% confidence limits) was 3.4 × 10⁶ (9.3 × 10⁵ − 6.9 × 10⁶) conidia ml⁻¹. This study showed that P. fumosoroseus (isolate no: 2679) could be a good candidate as a microbial control agent against T. pityocampa in forest environment.     

Leaf eating insect damage on different poplar clones and sites

As much for the geneticist as for the planter, information on sites, clones, and other factors would be decisive to reduce insect damage on poplars. In this perspective, a study was done in eight field trials in Belgium and in Luxembourg, considering two types of leaf damage on 24 clones from Italy, Belgium and the Netherlands. Damage varied considerably, depending on the clones, sites, species and parental individuals. Clone’s ranks concord on the different sites; site’s ranks are similar for the various clones. Clones with same parents have similar levels of resistance. The two types of damage are not correlated. Hybrids of P. deltoides × nigra show widespread variability; those with genetic material of P. trichocarpa are generally more resistant to caterpillar-like damage. The maximal differences are about 1 to 5 for the clones, and 1 to 10 for the sites. But the most vulnerable clone on the most favourable site was damaged almost 70 times more often than the opposite combination.     

黑杨派基因库内无性系生长特性的遗传分析*

黑杨派基因库内无性系生长特性的遗传分析解荷锋，于中奎，陈一山，张绮纹，徐红关键词黑杨派，基因库，生长特性，遗传变异黑杨派杨树在杨属栽培种中速生性能及经济价值最高［１］，其中尤以美洲黑杨为佳。我国从长江流域到黑龙江以南，广大平原地区都适宜生长［２］。为...     

Comparison of postfire mortality in endemic Corsican black pine (<Emphasis Type="Italic">Pinus nigra</Emphasis> ssp. <Emphasis Type="Italic">laricio</Emphasis>) and its direct competitor (<Emphasis Type="Italic">Pinus pinaster</Emphasis>)

• Introduction   

Laricio pine (Pinus nigra J.F. Arn. ssp. laricio (Poiret) Maire var Corsicana Hyl.) is a form of black pine endemic to Corsica, that may now be under threat due to current fire regimes and competition with maritime pine (Pinus pinaster Aiton).     

Dünnschichtchromatographische und mikrobiologische Untersuchungen über den Hemmstoffgehalt einiger Pappelklone

Investigations of the content of inhibitory substances in some Poplar clones by thin-layer chromatography and the use of micro-organisms . Poplar leaves and stems (T 39 Wettstein Züchtung, T 213 PבForndorf’, T 217 Pבvernirubens’) from fertilized plots (P N K) and unfertilized plots were tested microbiologically and by thin-layer chromatography. Leaf extracts contained phenolic compounds which influenced the growth of Septotinia podophyllina Whetz. Stem extracts were found to contain other substances influencing the growth of Cryptodiaporhthe, populea (Sacc.) Butin. Fertilizing frequently influenced phenol production in bark extracts but only seldom in leaf extracts.     

Can soil plant analysis development values predict chlorophyll and total Fe in hybrid poplar?

Iron chlorosis caused by an elevated soil pH remains an important selection criteria in evaluating hybrid Populus trials in northwest New Mexico. Compared to expensive extraction methods or visual ranking scales, the soil plant analysis development (SPAD) chlorophyll meter is a handheld tool that objectively indicates leaf color. SPAD calibration curves pertaining to Fe status in Populus leaves were developed for two clones: NM-6 (Populus nigra × Populus maximowiczii) and OP-367 (Populus deltoides × Populus nigra). Hybrids were grown in the greenhouse in a sandy loam soil of pH > 8 and the same soil amended with composted biosolids enriched with 420 mg kg⁻¹ Fe. SPAD measurements related to foliar Fe as follows: r ² = 0.72 for NM-6 and r ² = 0.58 for OP-367. SPAD measurements correlated with total chlorophyll as follows: r ² = 0.66 for NM-6 and r ² = 0.85 for OP-367. Based on the total chlorophyll content (supported with foliar element data), Fe sufficiency threshold values from SPAD measurements were estimated in these two clones.