“800”浓缩料替代鱼粉饲喂肉猪的效果

为验证“800”浓缩料替代鱼粉喂猪效果以便推广应用,选用三元杂交猪24头,随机分为试验和对照组进行观测。结果,试验组平均日增重668g,提高12．65％;料肉比3．83,降低6．13％;每kg增重节省饲料费10．1％,提高经济效益73．1％。     

A Comparative Study of the Preventive Use of Tilmicosin Phosphate (Pulmotil premix®) and Mycoplasma hyopneumoniae Vaccination in a Pig Herd with Chronic Respiratory Disease

This study was conducted to compare the effects of a preventive in‐feed medication programme using tilmicosin (Pulmotil^® 200 premix, Elanco Animal Health) at 200 p.p.m. with those of a Mycoplasma hyopneumoniae (Mh) vaccination programme (Stellamune^TM Mycoplasma, Pfizer Animal Health). A pig herd with chronic respiratory disease in which infection with Mh played an important role was selected, and a total of 204 piglets were randomly allocated to either the medication (P) or the vaccination (V) group. Pigs in the P group received medicated feed for 3 weeks after weaning (days 34–55), and for 2 weeks late in the nursery period (days 77–98). The piglets in the V group were vaccinated twice intramuscularly, at 4 and 22 days of age. The two groups were compared on the basis of average daily gain (ADG), feed conversion rate (FCR), additional curative medication days (CMD), overall mortality (major variables), a coughing index, pneumonia lesions, and serology against Mh, influenza H1N1 and influenza H3N2 viruses, Actinobacillus pleuropneumoniae (App) and porcine reproductive and respiratory syndrome virus (PRRSV) (minor variables). No significant differences (P > 0.05) were observed for ADG (555 g/day in P group; 567 g/day in V group), FCR (2.64 in P group; 2.41 in V group) and mortality rate (11% in P group; 7% in V group). The average number of additional curative medication days (CMD) per pig was significantly higher (P < 0.01) in the P group (1.5) than in the V group (0.58). At slaughter age, the serological results and the prevalence of macroscopic lung lesions were comparable in the two groups (P > 0.05). With the exception of CMD, the preventive use of tilmicosin at this swine farm was found to confer similar beneficial effects to Mh vaccination.     

饲料添加剂和添加剂预混合饲料生产许可证获证企业名单(广东省部分)

最近,农业部发布第 140号公告,公布了第 7批获得饲料添加剂和添加剂预混合饲料生产许可证的企业名单。现将其中我省获得饲料添加剂和添加剂预混合饲料生产许可证的企业名单刊发如下,供饲料行业的生产者、经营者以及消费者参考。     

我省公布饲料添加剂和添加剂预混合饲料产品批准文号(2004.8～2005.1)(上)

根据《饲料和饲料添加剂管理条例》和《饲料添加剂和添加剂预混合饲料产品批准文号管理办法》的有关规定,2004年8月至2005年1月,我省共核发广州市骏泰生物饲料有限公司等24家企业64个饲料添加剂产品批准文号,广州市骏泰生物饲料有限公司等34家企业386个添加剂预混合饲料产品批准文号。特此公告。     

An Outbreak of Salmonella enteritidis Infection in Pygmy Hogs (Sus salvanius)

An outbreak of salmonellosis was recorded in captive pygmy hogs (Sus salvanius), a critically endangered species of mammal. Of 42 captive animals maintained for conservation breeding by the Pygmy Hog Conservation Programme, Guwahati, Assam, India, 7 (16.67%) died within 3 days. The organism associated with this outbreak was identified as Salmonella enteritidis. The organisms were highly susceptible to chloramphenicol, gentamicin, norfloxacin and cefotaxim but were resistant to ampicillin, oxytetracycline, mezlocillin and sulfamerazin. The strain belonged to phage type 13a/7 and harboured two plasmids (38 and 44 megadaltons). The organisms were enterotoxigenic in CHO cell assay and were found to carry stn, sef and pef genes.     

发酵床猪舍对育肥猪生长性能及肉品质的影响

选择46 kg左右的杜长大三元杂交猪60头,随机分成两组(试验组,发酵床猪舍;对照组,传统水泥地面猪舍),每组3个重复,每个重复10头猪,在日粮配方相同的情况下进行饲养试验、屠宰测定和肉品质量检测.结果表明:日增重(840～874 g)、料肉比(3.01～3.1),试验组略优于对照组,而饲料成本试验组比对照组每头降低20.7元(按增重90 kg计算);眼肌面积、瘦肉率试验组比对照组分别提高21.5%(P<0.05)和3.04%.发酵床猪舍对猪肉品质及肌肉化学成分、氨基酸含量无明显影响,但能显著提高肌肉中粗脂肪、精氨酸的含量,粗脂肪提高15.12%(P<0.05),精氨酸提高12.58%(P<0.05);发酵床猪舍不会造成肌肉中铜、铅、砷、汞、镉、铬的残留超标.结果显示,采用发酵床养猪技术能改善胴体品质,节省用水,特别是猪舍臭味明显减少,无粪尿污水外排.     

Listeria monocytogenes Infection in a Sugar Glider (Petaurus breviceps) – New Mexico, 2011

Listeria monocytogenes is a Gram‐positive, facultative anaerobic, rod‐shaped bacterium that can infect and cause disease in many species. In this case report, we describe a case of L. monocytogenes infection causing sepsis in a sugar glider (Petaurus breviceps). The sugar glider consumed a varied diet consisting of human food items, including cantaloupe. A nationwide outbreak of L. monocytogenes foodborne illness associated with cantaloupes occurred simultaneously with this incident case. In this case, the bacterial strains from the outbreak and glider were genetically distinct. Although rare, veterinarians should be aware of the emergence of foodborne pathogens' ability to infect exotic animals residing in domestic environments.     

First Evidence of Porcine Circovirus Type 2 (PCV‐2) Infection of Pigs in the Czech Republic by Semi‐Nested PCR

Three oligonucleotide primers for semi‐nested polymerase chain reaction (PCR) were designed according to already published sequences of porcine circovirus types 1 (PCV‐1) and 2 (PCV‐2) isolates. These primers were used to detect PCV‐2 DNA. A positive amplification reaction was visualized from a DNA suspension containing as few as 10 copies of virus DNA. In total, 77 samples of inguinal lymph nodes and nasal swabs from pigs in the Czech Republic were used to detect the virus. Thirty‐seven of them were positive for PCV‐2 DNA. In order to confirm specificity of the PCR reaction, seven DNA fragments were sequenced. Czech PCV sequences were found to have a 92–97% homology with other known PCV‐2 strains and only 80–83% homology with PCV‐1 strains.     

Efficacy and toxicity of carboplatin and cytarabine chemotherapy for dogs with relapsed or refractory lymphoma (2000–2013)

Medical records of 22 dogs treated with carboplatin (n = 8) or carboplatin and cytarabine (n = 14) chemotherapy for relapsed or refractory lymphoma between 2000 and 2013 were retrospectively reviewed. The clinical response rate was 18.2% (4/22). Median time to progression was 18 days (56 for responders; 12 for non‐responders, P = 0.0006). Median overall survival time was 28 days (109 for responders; 21 for non‐responders, P = 0.0007). Thrombocytopenia and neutropenia occurred in 84.2% (16/19) and 52.6% (10/19), respectively. Grade IV thrombocytopenia and neutropenia occurred in 56.3% (9/16) and 60.0% (6/10), respectively. Dogs that received both drugs were more likely to become neutropenic (P = 0.022) or thrombocytopenic (P = 0.001) than dogs receiving carboplatin alone. All responders received both drugs giving a 28.6% (4/14) response rate for the combination. Although some dogs responded to the combination, toxicity was high and the responses were not durable. With adequate supportive care, this protocol may be an acceptable rescue option for some dogs.     

Treatment of oromaxillary fistulae in nine standing horses (2002–2006)

Reasons for performing study: There is minimal published information on equine oromaxillary fistulae that are unrelated to cheek teeth (CT) repulsion or on the conservative treatment of these atypical fistulae. Objectives: To report equine oromaxillary fistulae unrelated to CT extraction and describe their management in standing horses. Methods: Case details of oromaxillary fistulae of atypical aetiology occurring at 2 referral centres between 2002–2006, including their treatment and response to treatment were examined. Results: Nine cases of oromaxillary fistula were recorded, mainly in aged horses (median 22 years). Fistulae were associated with CT diastemata in 7 cases, fractured CT in one and a central defect in a worn CT in another. After removing food and exudate from the sinuses, 6 cases were treated successfully by filling the diastema or dental defect with polymethylmethacrylate (PMMA). Following dental extraction, the other 3 cases were treated successfully by use of PMMA alveolar packing. Conclusion: Older horses can spontaneously develop oromaxillary fistulae, usually secondary to CT diastemata. In the absence of apical infection, this disorder can usually be treated successfully in standing horses by treating the sinusitis and sealing the oral aspect of the diastema with PMMA. Potential relevance: Older horses with sinusitis should be assessed for the presence of CT diastemata and oromaxillary fistulae. If detected, these disorders can be treated successfully in the standing horse.     

An Outbreak of Enzootic Bovine Leukosis in Upper Egypt: Clinical,Laboratory and Molecular–Epidemiological Studies

In 1989, 220 Holstein Friesian cattle (212 heifers and eight bulls) were imported from Minnesota, USA, to form a closed dairy herd in Arab El‐Aoumar, Assiut, Upper Egypt. In November 1996, some abnormal signs such as loss of weight, decreased milk yield, external lymphadenopathy and decreased appetite were observed on this farm. Serological screening by enzyme‐linked immunosorbent assay revealed a seroprevalence of antibodies directed against bovine leukaemia virus (BLV) of 37.7% in cattle under 2 years old and of 72.8% in animals more than 2 years old. Diagnosis was confirmed by the detection of BLV proviral DNA using polymerase chain reaction with primers amplifying a fragment of the env gene. Out of 21 tested leucocyte fractions from individual animals, 15 were positive showing a BLV‐specific amplicon of 444 base pairs. Analysis of the amplicons for restriction fragment length polymorphisms and DNA sequencing results allowed the isolates to be typed. Since this was the first recorded case of enzootic bovine leukosis in Upper Egypt, strict quarantine measures were adopted and all serologically positive animals in the herd were culled.     

板芩桔甘合剂对传染性支气管炎病毒人工感染雏鸡的临床疗效分析

旨在探讨板芩桔甘合剂（BQJGHJ）对传染性支气管炎病毒（IBV）人工感染雏鸡的临床疗效。选取120只雏鸡随机分为空白组、模型组（感染病毒）、阳性药物组（感染病毒+甘胆口服液）和BQJGHJ高、中、低剂量组（感染病毒+BQJGHJ）,每组20只。除空白组外,其他组感染IBV造模,4 d后,各组分别饮水给药,连用5 d。每天观察各组雏鸡临床症状,记录临床症状记分,统计有效率、治愈率;在造模后,给药第3、5天,及停药后第2天分别自翅下静脉采集血液,分离血清,ELISA法检测感染雏鸡血清中IFN-γ、IL-6、IL-10含量变化。结果显示：BQJGHJ高、中、低剂量组的治愈率分别为75.0%、80.0%、65.0%,有效率分别为90.0%、95.0%、80.0%。模型组雏鸡血清中IL-10含量降低,IL-6含量升高,与空白组比较,差异显著（P<0.05或P<0.01）;在给药第3、5天时,BQJGHJ高、中、低剂量组、阳性药物组雏鸡血清中IFN-γ（第5天时,高剂量组差异极显著,P<0.01,其他时间、其他组差异均不显著）、IL-10含量（第5天时差异极显著,P<0.01）升高,雏鸡血清中IL-6含量降低,与模型组比较,均差异显著（P<0.05）。在停药后2 d,BQJGHJ高、中、低剂量组、阳性药物组雏鸡血清中IFN-γ、IL-10含量升高,雏鸡血清中IL-6含量降低,与模型组比较,均差异显著（P<0.05或P<0.01）;BQJGHJ高、中、低剂量组雏鸡血清中IFN-γ、IL-6、IL-10含量,与阳性药物组相比,无显著性差异（P>0.05）。BQJGHJ可提高传染性支气管炎雏鸡血清中IFN-r、IL-10的含量,降低传染性支气管炎雏鸡血清中IL-6的含量,发挥治疗鸡传染性支气管炎的作用。     

Lymphangiosarcoma in 12 dogs: a case series (1998–2013)

Lymphangiosarcoma (LAS) is an uncommon malignant neoplasia arising from lymphatic endothelium; little information exists regarding therapy. Single institutional retrospective review for canine LAS histopathology diagnoses over a 15‐year period yielded 12 dogs. Ten dogs were presented for a mass and/or swelling at cervical, trunk or limb regions. Prior to diagnosis, 10 dogs received empiric wound therapy. Cytology performed in 10 consisted of mild inflammation. Survival ranged from 60, 168 and 876 days for three dogs with palliation; 90 days with prednisone in one; 182 days with chemotherapy in one; 240, 267, 487, 630 and 941 days for five receiving surgery; and 574 days for one receiving surgery, radiation and chemotherapy. One dog is alive with recurrence at 243 days following surgery and carboplatin chemotherapy. Clinical improvement existed in LAS dogs receiving multimodal therapies. Early tissue biopsies are recommended for progressive oedematous lesions of unknown origin.