2009年贵州部分引进辣椒品种区域田间试验

设9个试验点,以‘遵椒2号’作对照,对7个引进的辣椒品种开展区域田间试验,并对参试品种的性状数据进行综合分析。结果表明:有5个品种比对照种增产0.21%～1 2.04%,其中增产幅度达极显著水平的品种有3个,有2个品种增产幅度差异不显著;有2个品种比对照种减产1.03%～4.09%,减产幅度差异水平均未达到显著水平。     

2010—2011年广西合浦甘蔗品种区域试验简报

为了探讨广西区已育成或引入的甘蔗新品种（系）在广西合浦县生态区域的适应性,2010—2011年间,在合浦县对10个参试材料和2个对照品种进行了新植试验研究。结果表明：桂糖04-2278平均蔗茎产量和含糖量最高,增产效果比CK1和CK2分别达到了显著和极显著水平。桂辐98-296的平均蔗茎产量和含糖量均高于两对照,比CK2增产极显著,但比CK1达不到显著水平。桂糖04-2724的平均蔗茎产量和含糖量均高于两对照,比CK2增产显著,但比CK1达不到显著水平。桂糖04-120的平均蔗茎产量高于两对照,但增产不显著,并且平均甘蔗蔗糖分低于两对照,在所有参试品种（系）中,蔗糖分最低。其它品种（系）的甘蔗蔗茎产量及蔗糖分表现均不突出。     

贵州地方辣椒品种比较试验

以遵椒2号作对照,对7个贵州地方常规干制辣椒品种进行多点比较试验。试验结果表明,有6个品种比对照增产3．01％-37．77％,其中增幅达到显著或极显著水平的品种有4个;有1个品种比对照减产7．38％,减幅差异不显著。其中表现最好的品种是224,不仅产量高,而且稳产性最好,适宜推广种植;其次是204,产量较高。     

部分鲜食辣椒品种2009年贵州区域试验

以辛香二号作对照,对6个鲜食辣椒品种在贵州9个试点进行田间区域试验,并对参试品种的性状数据进行综合分析。结果表明,有5个品种比对照种增产,增幅达10.03%～32.41%,其中产量差异达极显著水平的品种有4个（长研201,韩玉109,早青109,永利209）,达显著水平的品种有1个（黔椒4号）;有1个品种（新甜椒一号）比对照种（辛香二号）减产10.87%,减产幅度差异达到显著水平。     

不同南瓜砧木对薄皮甜瓜生长、产量及品质的影响

用6个不同南瓜砧木品种嫁接薄皮甜瓜,研究南瓜砧木对薄皮甜瓜生长、产量及品质的影响。结果表明,各嫁接组合抗枯萎病能力、产量方面都显著提高,但抗白粉病能力及品质方面都有所降低。综合各方面看,选用砧木达美嫁接,嫁接苗成活率高,嫁接后甜瓜产量显著提高,比对照增产49.4%,抗枯萎病能力显著增强,中心含糖量和口感风味与对照相当,可作为今后嫁接栽培的理想砧木品种。     

贵州辣椒区试G组田间比较试验

设4个试验点，以辛香2号品种作对照，对6个引进的菜椒品种开展区域田间比较试验，并对参试品种的性状数据进行综合分析。结果表明：参试的6个品种比对照种增产0．89％～44．57％，增幅达极显著差异水平的品种有4个，增幅差异水平不显著的品种有2个。     

2009-2010年海南(三亚)甜瓜品种比较试验

为了鉴定甜瓜品种的丰产性、稳产性、适应性、抗逆性及其他重要品种特性,筛选适合不同生态区域、不同栽培方式和综合性状优良及适合设施栽培的甜瓜品种,笔者引进和征集了光皮类型和网纹类型甜瓜品种253个,进行品种比较试验。根据其综合表现,从中筛选出表现突出的光皮类型甜瓜品种10个,网纹类型甜瓜品种12个,推荐参加2010-2011年海南(三亚)甜瓜品种生产试验,为甜瓜新品种推广提供依据。     

韭菜早春保护地适宜品种的研究

通过对韭菜10个品种早春保护地栽培单株产量构成性状的研究表明，平韭5号发苗期最短，分别比其他品种早出苗2－3天。单株高、叶片长、鞘长、鞘粗、单株重与其他品种差异均达极显著水平，小区产量577g，比对照嘉兴白根增产198.96%，比平韭4号增产118.56%，比其他品种增产116.51%－306.33%。     

福建省厚皮甜瓜新品种比较试验初报

2002年对自育的4个厚皮甜瓜品种(组合)进行了品种比较试验,露地栽培,以伊丽莎白为对照。结果显示:供试的4个品种(组合)嘉蜜、嘉美、68×121、126×114种性优良,耐湿性好,各具特色,熟性虽比对照略晚,但均属于早熟品种,且综合性状均略优于对照。     

适宜南通地区大棚栽培的厚皮甜瓜品种筛选

为筛选出适宜南通地区栽培的厚皮甜瓜品种,选用5个光皮类型和6个网纹类型的厚皮甜瓜品种进行品种比较试验。结果表明:光皮类型中的海蜜6号和一品红,网纹类型中的抗病3800和海蜜5号这4个厚皮甜瓜品种具有丰产、优质、商品性好等特点,可作为主栽品种在南通地区进行推广。     

茶薪菇品种比较实验研究

对7个茶薪菇品种进行品种比较实验从中选择出适宜于棉籽壳为主的培养料的优质菌株5个，瓶栽实验生物学效率显著地高于对照品种的生物学效率(53．45％)，达到1％的显著水准。     

光质对矮生黄瓜生长及生理特性的影响

以矮生黄瓜D0462和蔓生黄瓜129为试材,研究不同光质对其形态指标和生理特性的影响。结果表明,蓝光和红光处理对129和D0462均有影响,且蓝光影响更大;D0462经蓝光处理后下胚轴长极显著低于129,除叶绿素含量两品种间差异不显著外,D0462的花色素苷含量、IAA氧化酶、SOD及POD活性经蓝光处理后均显著高于129。     

Differentiation of rat bone marrow mesenchymal stem cells expressing hepatocyte growth factor into hepatocyte-like cells

AIM:To observe the hepatic differentiated efficiency of rat bone marrow mesenchymal stem cells (rMSCs) expressing hepatocyte growth factor (HGF) in three-dimensional microenvironment formed by hanging drop. METHODS:rMSCs were isolated and cultured in vitro, and flow cytometry was used to detect the expression of CD44, CD90, CD34 and CD45. Recombinant retrovirus carrying cDNA of human HGF (pLNCX2-hHGF) was constructed and infected with rMSCs (hHGF-rMSCs). hHGF expression in hHGF-rMSCs was detected by RT-PCR, immunofluorescence staining and ELISA. hHGF-rMSCs were cultured in hanging drop for 21 days. The expression of albumin (ALB),cytokeratin-18 (AFP) and alpha fetoprotein (CK-18) were detected by RT-qPCR and immunofluorescence staining in the 7th, 14th and 21st day, respectively. The secretion of albumin in cultured supernatant was measured by ELISA. RESULTS:CD44 and CD90 were highly expressed in the third generation of rMSCs, but CD34 and CD45 were hardly expressed. The expression of hHGF at mRNA and protein level were all detectable in the hHGF-rMSCs, and the secretion in the cultured supernatant was about (123.71±8.81) μg/L in a period of 21 days. In the hHGF-rMSCs, ALB, AFP and CK-18 were highly expressed at mRNA level from the 7th to the 21st day, and there were significant differences compared with rMSCs at the same time point (P<0.01). The results of immunofluorescence staining showed that the protein expression of AFP was negative on day 7 and 14, and positive on day 21; while the protein expression of ALB and CK-18 was positive on day 7 and lasted until day 21. ALB was positively observed in the culture supernatant of hHGF-rMSCs from 7th to 21st day measured by ELISA, and there was significant difference between the hHGF-rMSCs and rMSCs (P<0.01). CONCLUSION:hHGF transduced-rMSCs can be induced to differentiate into hepatocyte-like cells after cultured in hanging drop which provides a three-dimensional microenvironment. All these results might help to provide new seed cells for cell therapy of clinical liver diseases and in vitro bioartificial liver.     

大白菜不同品种对硝酸盐积累差异的研究

 将17个大白菜品种在高浓度氮素水平下营养液栽培, 分析各品种不同时期硝酸盐含量, 发现不同品种之间硝酸盐含量差异达到极显著, 最高和最低者相差两倍以上。硝酸还原酶的活性同硝酸盐含量相关性达到显著负相关, 但是也有硝酸盐含量低, 同时硝酸还原酶活性也低的品种, 通过分析认为可以通过育种途径培育出硝酸盐低积累量的品种。     

二十五个三色堇和角堇品系性状比较

收集国内外大花型、中花型、小花型三色堇及角堇材料共25份,对其发芽、开花特性及株高、冠幅、花径、花量等主要观赏性状进行比较研究。结果表明:美国品种(系)观赏性状较好,国内品种(系)适应性更强。供试材料中白色、黄色褐斑比红色、红色褐斑三色堇生长更健壮,综合表现更好。大花型三色堇冠幅和花径较大,中、小花品种花朵繁密,盛花期持续时间长,可根据具体要求合理栽植。     

几个日本大葱品种苗期耐热性鉴定评价

以章丘大葱为对照,对引自日本的不同大葱品种进行了耐热性鉴定。结果表明,高温胁迫使大葱叶片电解质渗漏率及丙二醛(MDA)含量增加,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等保护酶活性变化较大。热害指数随高温胁迫时间延长而上升,但不同品种的变化幅度不同,表明不同大葱品种的耐热性存在差异。通过计算隶属函数值的方法,对高温胁迫下大葱各生理指标进行了综合评价,田喜与长宝的耐热性较强,显著高于章丘大葱,而春味与元藏的耐热性则较差,高冠与章丘大葱差异不大。     

杏果实糖酸组成及其不同发育阶段的变化

 采用高效毛细管电泳仪分离测定了10个杏品种果实中的糖酸组分, 并对新世纪杏与凯特杏不同发育时期糖酸组分的变化进行了研究。结果表明: 各糖组分及总糖含量在品种间都存在较大差异, 但均以蔗糖含量最高, 果糖含量最低; 苹果酸和柠檬酸在品种间差异显著, 根据有机酸组成不同, 可将杏品种分为柠檬酸型和苹果酸型; 在果实不同发育时期, 两个品种果实中总糖含量及各组分的变化趋势基本一致,总糖和蔗糖的含量一直在增加, 而果糖和葡萄糖的含量变化不大。新世纪杏中总酸和苹果酸含量变化趋势完全一致, 都在较高水平上缓慢增加, 而柠檬酸的含量始终很低; 凯特杏中柠檬酸在果实发育前期含量很低, 硬核期结束时含量急剧上升, 成熟前略有下降, 呈“S”型变化曲线, 而苹果酸的变化规律正好与柠檬酸相反, 总的变化趋势呈现反“S”型变化曲线。     

Hepatic differentiation of mesenchymal stem cells derived from rats in three-dimensional microenvironment formed by hanging drops

AIM: To establish an optimal differentiated three-dimensional microenvironment formed by hanging drops for the high efficiency of hepatic differentiation from rat mesenchymal stem cells(rMSCs).METHODS: rMSCs were cultured in the hanging drops, which provided a three-dimensional microenvironment, for 21 days in the presence of hepatocyte growth factor(HGF, 20 μg/L). The expression of albumin(ALB), alpha-fetoprotein(AFP) and cytokeratin-18(CK-18) was detected by RT-PCR and immunofluorescence staining at 7th, 14th and 21st days. The secretion of albumin in the culture supernatants was measured by ELISA. RESULTS: rMSCs were aggregated in spheroid with a tubiform medium altitude in the center. In rMSCs cultured in the hanging drops with HGF, the expression of albumin, AFP and CK-18 was all detectable by RT-PCR and immunofluorescence staining at 7th day. The production of albumin in the cells cultured in the hanging drops with HGF was 50.25±5.32, 55.03±7.45 and 54.92±3.18(ng·dish^-1·d^-1) at 7th, 14th and 21st days, respectively, significantly higher than that in the cells in the plate cultivation with or without HGF induction at corresponding time points(P<0.01).CONCLUSION: In the presence of HGF, rMSCs are induced to differentiate into hepatocyte-like cells cultured in the hanging drops, where the three-dimensional spheroidal cultures are promising microenvironment for hepatic transdifferentiation of MSCs.