小尾寒羊同期发情与人工授精试验研究

在本试验中,运用氯前列醇注射法和阴道栓法可使小尾寒羊母羊同期发情率分别达到83.3%和90.0%,虽然后者的发情率比前者高6.7%,但是差异不显著。使用人工授精方法可使母羊情期受胎率和分娩率分别达到82.9%和86.1%,比自然交配分别提高6.1%和3.5%。通过同期发情和人工授精技术的结合,可达到提前配种和计划配种的目的,同时可扩大种公羊的利用率、减少饲喂种公羊的成本,在生产实践中值得进一步推广。     

肉羊同期发情控制技术

1 同期发情技术的意义和注意事项 1．1同期发情技术的意义减少发情鉴定时间和次数,合理利用圈舍、气候和草场资源,提高优秀种公羊的利用率。在绵羊高效繁殖体系中,对母羊实行一年两产或两年三产,依市场需求调整母羊配种期和产羔期。在草场或配种时间有限制的地区,母羊批量发情,便于人工授精和品种改良的计划组织和实施。     

肉羊同期发情人工授精关键技术

肉羊同期发情人工授精技术是指通过激素等方法人为控制母羊发情进程,使母羊群集中于特定时间段同时发情、排卵,应用优良种公羊精液对同期发情母羊群进行集中人工输精,实现肉羊批量化生产的高效繁殖技术。推广应用该技术可以使母羊集中发情、集中输精、集中分娩、统一管理、统一出栏,对提高空怀母羊和优良种公羊配种效能、降低生产成本、实现肉羊科学化、规范化、标准生产、提高肉羊生产经济效益具有重要意义。     

肉羊同期发情人工授精技术

<正>肉羊同期发情人工授精技术是指通过激素等方法人为控制母羊群发情进程,使母羊群集中于特定时间段同时发情、排卵,应用优良种公羊精液对同期发情母羊群进行集中人工输精,实现肉羊批量化生产的高效繁殖技术。推广应用该技术可以使母羊集中发情、集中输精、集中分娩、统一管理、统一出栏,对提高空怀母羊和优良种公羊配种效能,降低生产成本,实现肉羊科学化、规范化、标准生产,提高肉羊生产经济效益具有重要意义。     

不同药物同期发情处理与温精授配对比试验

用前列腺素、三合激素、孕马血清促性腺激素，结合注射法进行小尾寒羊同期发情处理，不同药物间差异显著（P〈0．05），以孕马血清促性腺激素处理效果最好；采取无角道塞特、萨福克公羊精液稀释后进行子宫颈输精配种，以孕马血清促性腺激素处理小尾寒羊的情期受胎率比前列腺素、三合激素分别提高了30．65％、24．80％。试验表明，绵羊同情发情使用PMSG效果最好。     

羊同期发情技术的应用

同期发情,即有计划地使一群羊或一个自然村的当地母羊在同一时间内发情,这便于羊的人工授精,提高精液利用率。同时,母羊同期发情、同期配种、同期产羔也便于生产的组织和管理工作。公羊1次射出的精液稀释后,可供50～100只母羊输精。在普通条件下,精液保存的时间不超过3d,如果母羊零散地发情,每次采集的精液只能利用一小部分,不但造成精液浪费．而且增加种公羊不必要的负担和精力消耗。     

非繁殖季节诱导流产细毛羊同期发情的试验

对183只流产细毛羊在非繁殖季节诱导同期发情,用处理公羊配2个情期,结果有71.6%的处理母羊产羔,并且这些产羔母羊能够及时参加当年繁殖季节的配种。     

非繁殖季节诱导流产细毛羊同期发情的试验

对183只流产细毛羊在非繁殖季节诱导同期发情，用处理公羊配2个情期，结果有71.6％的处理母羊产羔，并且这些产羔母羊能够及时参加当年繁殖季节的配种。     

晋岚绒山羊同期发情和人工授精技术的应用

同期发情配合人工授精技术,不仅可大幅度提高优秀种公羊利用率,在短时间内完成配种任务,也便于档案记录和管理,为育种工作创造条件。2012和2013年,我们采用两次前列腺素注射法共处理晋岚绒山羊母羊10 304只,同期发情率平均为86.7%,共对8 930只母羊进行了输精,平均受胎率为93.0%,效果良好,促进了晋岚绒山羊新品种的选育提高。     

非繁殖季节诱导琉产细毛羊同期发情的试验

对183只流产细毛羊在非繁殖季节诱导同期发情，用处理公羊配2个情期，结果有71．6％的处理母羊产羔，并且这些产羔母羊能够及时参加当年繁殖季节的配种。     

水牛同期发情技术的研究

采用不同药物处理和给药方式,对不同品种水牛在不同季节自然发情和同期发情配种后的受胎效果进行系统研究,以期建立一套稳定的适合水牛同期发情的处理方法。试验选取本地水牛(102头)、摩拉水牛(129头)、尼里/拉菲水牛(98头)和杂交水牛(326头)共655头,分为5个组进行比较试验。结果表明:河流型摩拉水牛和尼里/拉菲水牛的同期发情率和配种受胎率均高于杂交水牛和本地水牛(P<0.05),但同为河流型的摩拉和尼里/拉菲水牛之间没有差异(P>0.05),以本地水牛效果最差,其同期发情率和配种受胎率分别为74.51%和30.39%;应用不同的药物处理时,以GnRH+PGF2α+GnRH效果最好,同期发情率和受胎率分别为88.46%和46.38%,其同期发情率显著高于其他各药物组(P<0.05),而用PGc的效果最差(分别为79.10%和33.21%);用PMSG肌注+PGc灌注法,其同期发情率和受胎率效果最好,显著高于其他各组(P<0.05),其次是PMSG肌注+PGc肌注法,PGc肌注法效果最差;在冬季进行同期发情处理时,同期发情率和受胎率最高(分别为83.75%和43.28%),明显高于其他各季节(P<0.05);水牛自然发情的人工授精受胎率和胚胎移植的受胎率均比同期发情的高,差异显著(P<0.05)。     

肉牛同期发情效果影响因素的研究

[目的]为了研究同期发情处理方法、繁殖状况、品种、体重等对肉牛同期发情效果的影响,[方法]选择140头不同繁殖状况、品种、体重的肉牛,随机分为3组,分别采用CIDR+第10天肌注PMSG+第11天肌注PGF_2α（埋栓Ⅰ组）、CIDR+第9天肌注PMSG+第11天肌注PGF_2α（埋栓Ⅱ组）、肌注PGF_2α+PMSG(PGF_2α组)的方法进行同期发情处理。[结果]3种不同处理方法相比,PGF_2α组发情率为87.10%,埋栓Ⅰ组、Ⅱ组母牛发情率分别为97.83%、98.41%,显著高于PGF_2α组（P<0.05）;PGF_2α组处理牛受胎率为83.87%,埋栓Ⅰ组、Ⅱ组处理牛受胎率分别为89.13%、84.13%,高于PGF_2α组,但差异不显著（P>0.05）;育成牛与经产牛发情时间相比,埋栓组12～24 h内出现发情症状的经产牛占比为27.27%,育成牛占比为60%,显著高于经产牛（P<0.05）,2...     

马的同期发情试验

试验利用脂肪酸类激素药物PGF2α来诱导母马同期发情。试验马匹分为发情正常,生殖系统健康组;无发情表现,无子宫内膜炎,有黄体组;无发情表现,无子宫内膜炎,无黄体组。结果表明:发情正常、生殖系统健康组的受胎率高,发情不正常的马匹同期发情的效果不好,即使发情,受胎率也不高。     

甘肃马鹿同期发情效果初步研究

将45头甘肃马鹿均分为3组,应用氯前列烯醇(PG)和孕马血清促性腺激素(PMSG)进行同期化处理,处理A组其处理程序为PG+PG+PMSG,处理B组其处理程序为PG+PMSG。结果表明:A、B组5d内的同期发情率(分别为93·3%和86·7%)均高于对照组(P<0·05),但2个处理组间差异不显著(P>0·05);产仔率分别为73·3%和60·0%,比对照组分别提高20%和6·7%,其中A组的双仔率为6·7%;产仔日期比对照组分别缩短了12d和8d。处理组仔鹿的平均初生重也比对照组提高1·53kg,而且A、B2组仔鹿的断奶成活率也比对照组分别提高了2·5和12·5个百分点。总体而言,A组效果较好。     

Use of norgestagens for estrus synchronization if farm animals

The action of norsteroid type preparations and their capability of synchronising oestrus in heifer and sheep were studied at laboratory and clinical levels. The effectiveness of norethysterone acetate in heifer was tested by subcutaneous injections of 5 mg and 10 mg and did not exceed the order of 50 per cent. Combination of norsteroids with serum of pregnant mares increased slightly the number of animals with synchronised oestrus and improved the rate of fertilisation. Studies conducted into sheep revealed that no complete inhibition of the sexual function was ensured by injection of norgestrol acetate with prolongator in intervals of four days. Implantation of norgestrol acetate was not effective either. Further studies are required, before the above preparations can be applied to farm animals on a large scale.     

A review of synchronization of estrus in postpartum cattle

Estrus has been synchronized in cattle with progestogens, prostaglandins (prostaglandin F2 alpha and its analogues), progestogen-estrogen combinations and progestogen-prostaglandin combinations. Progestogens administered for 14 to 20 d are effective in synchronizing estrus; however, fertility at the synchronized estrus is subnormal. Duration of progestogen treatment can be reduced by combining it with an estrogen. Syncro-Mate B is a progestogen-estrogen combination that results in a tightly synchronized estrous response. Prostaglandins can be used in double- or single-injection programs. Fertility of the estrus after prostaglandin treatment is similar to that of controls. Estrus also has been synchronized effectively by combining a 5- to 9-d progestogen treatment with prostaglandin at or near the end of treatment. When prostaglandin is used alone, cattle in the late stages of the luteal phase (d 11 to 15 of the estrous cycle) at the time of prostaglandin injection have a higher estrous response and may be more fertile than those injected with prostaglandin in the early part (d 6 to 9) of the luteal phase. More recently, a 14-d progestogen treatment has been combined with a prostaglandin injection given 16 to 18 d after progestogen withdrawal. This system places cattle in the late luteal phase of the estrous cycle at the time of prostaglandin injection and has resulted in an estrus with greater fertility than that immediately following progestogen treatment.     

Luteal lifespan and fertility after estrus synchronization in goats

The present experiment aims to examine the efficiency of estrus synchronization using progesterone and equine chorionic gonadotrophin (eCG) and to look at luteal function. During the non-breeding and breeding season, 5 adult female Korean native goats were injected intramuscularly with 2.5 ml of physiological saline as the control. A progesterone impregnated intravaginal sponge was then kept in the same goats for 10 days followed, after a week, by an intramuscular injection of 500 IU eCG. Five adult female Nubian goats were mated with a fertile buck during the non-breeding season. During the non-breeding season 2 of the 5 goats showed a normal estrous cycle (ranging from 18 to 21 days) and 3 a short estrous cycle (ranging from 3 to 6 days). During the breeding season the equivalent figures were 1 and 2. The major axes of the corpus luteum (CL) were measured by means of calipers built into the ultrasonography system, and the concentrations of plasma progesterone (P₄) were determined by double antibody radioimmunoassay. The mean major axes of the CL in goats showing the short cycle (6.1 ± 0.5 mm) was significantly smaller than in those showing the normal cycle (8.9 ± 0.5 mm; p < 0.01) and also the value of P₄ in goats showing the short cycle (4.2 ± 2.1 ng/ml) was significantly lower than for those showing the normal cycle (10.3 ± 4.3 ng/ml; p < 0.05) at day 3 following ovulation. Three out of 5 Nubian goats became pregnant but only one goat carried to full term. The present experiment indicated that a combination of progesterone and eCG was effective in inducing estrus, although it resulted in a high incidence of short luteal lifespan. The low kidding rate and high incidence of embryonic loss may be due to the instability of the luteal lifespan.     

Influence of estrus synchronization of prepubertal gilts on embryo quality

Synchronization and superovulation are commonly used to obtain large numbers of embryos for experimental and practical purposes. This study compared the number, quality, and in vitro development of embryos recovered from gilts following single or double estrus synchronization and superovulation. Prepubertal gilts from the single synchronization group were injected with 1500 I.U. PMSG and 1000 I.U. hCG 72 h later. The double synchronized group of gilts was treated with 750 I.U. PMSG and 500 I.U. hCG 72 h later. After 17 days, 1500 I.U. PMSG followed by 1000 I.U. hCG was administered. Five days after insemination embryos were recovered and cultured for 6 days. Both single and double hormonal stimulation schedules resulted in recovery of elevated numbers of embryos (28.4 and 23.4 vs. 11.3; p<0.01 and p相似文献