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1.
The currently accepted method of detection for azoxystrobin, a strobilurin fungicide, involves a labor-intensive organic solvent extraction and gas chromatography analysis. Three diagnostic assay formats, i.e., enzyme-linked immunosorbent assay (ELISA), fluorescence polarization (FP), and time-resolved fluorescence (TR-FIA), were developed and compared with regard to detection and quantification of azoxystrobin in grape extract and river, lake, and well water samples. These three assay formats require no initial sample extraction and were not affected by any of the environmental matrices tested, and each had a linear working range of 0-400 pg/mL. The polyclonal antibodies used for each of the immunoassays were specific to azoxystrobin; that is, the highest cross-reactivity to other pesticides observed was 5.7%. The limits of detection of the immunoassays were similar at 3 (ELISA), 46 (FP), and 28 (TR-FIA) pg/mL, as were the respective IC50 values of 306, 252, and 244 pg/mL. Each of the three immunoassays developed was less labor-intensive and approximately 100-fold more sensitive than the gas chromatographic method. While the three formats were comparable in terms of performance, the fluorescence polarization assay was the least labor-intensive and required the least time to perform.  相似文献   

2.
This study aimed at assessing the potential of near-infrared reflectance spectroscopy (NIRS) for determining the distribution of soil organic matter (SOM) in particle size fractions, which has rarely been attempted. This was done on sandy soils from Burkina Faso (three sites) and Congo-Brazzaville (one site). Over the total sample set, NIRS accurately predicted carbon (C) and nitrogen (N) concentrations (g kg?1 fraction) in the fraction <20 μm. When considering Burkina Faso only, predictions were improved in general; those of C and N amounts (g kg?1 soil) became accurate for the fraction <20 μm but not for the coarser fractions, probably due to heterogeneous SOM repartition. However, most SOM being <20 μm in general, NIRS could be considered promising for determining SOM size distribution.  相似文献   

3.
Pentachlorophenol (PCP) is used as a herbicide in agriculture and as an insecticide for termite control. Because of the apparent hazard associated with its usage, there is a need for an efficient and economic on-site screening method. A 5-min on-site test has been developed for the detection of PCP based on the OnTrak format, a successful Roche on-site test format for drugs of abuse, utilizing the principle of latex agglutination immunoassay. The test detects 1 ppm of PCP in soil samples.  相似文献   

4.
A liquid chromatography (LC) method with luminescence detection for the determination of eight quinolone antibiotics is reported. The system encompasses three consecutive steps: (a) chromatographic separation using reverse-phase mode (RP-LC), (b) postcolumn derivatization reaction, and (c) luminescence detection by monitoring fluorescence (FL) and time-resolved (TR) signals. The derivatization step is based on the reaction between quinolones and terbium(III) to form luminescent chelates, which were determined at lambda(ex) 340 and lambda(em) 545 nm (FL mode) or at lambda(ex) 281 and lambda(em) 545 nm (TR mode). Dynamic ranges of the calibration graphs, obtained with standard solutions of analytes and FL and TR modes, respectively, were 190-3500 and 316-2000 ng mL-1 for marbofloxacin, 8-3500 and 8.1-1500 ng mL-1 for ciprofloxacin, 6.2-3500 and 13-1500 ng mL-1 for danofloxacin, 7.4-3500 and 8.4-1500 ng mL-1 for enrofloxacin, 14-3500 and 20-2000 ng mL-1 for sarafloxacin, 12.5-3500 and 13.9-1200 ng mL-1 for difloxacin, 7.6-3500 and 13-3000 ng mL-1 for oxolinic acid, and 9-2000 and 130-3000 ng mL-1 for flumequine. Limit of detection values obtained using FL and TR modes, respectively, were 60 and 95 ng mL-1 for marbofloxacin, 2 and 2.4 ng mL-1 for ciprofloxacin, 1.9 and 3.9 ng mL-1 for danofloxacin, 2.2 and 2.5 ng mL-1 for enrofloxacin, 3.8 and 7 ng mL-1 for sarafloxacin, 4 and 4.2 ng mL-1 for difloxacin, 2.3 and 4 ng mL-1 for oxolinic acid, and 2.7 and 40 ng mL-1 for flumequine. The precision was established at two concentration levels of each analyte and expressed as the percentage of relative standard deviation with values ranging between 1.9 and 7.8%. The validation procedure for the analysis of samples was carried out using European Community recommendations, and the decision limit and detection capability were calculated for bovine whole milk. The method was applied to whole, semiskimmed, and skimmed milk samples spiked with the target analytes, and the recoveries ranged between 93.3 and 106.0%.  相似文献   

5.
A method based on high-performance liquid chromatography with diode-array detection was developed and validated aiming at the simultaneous determination of terbuthylazine (TER) and its five major metabolites, desisopropyl-hydroxy-atrazine, desethyl-hydroxy-terbuthylazine, desisopropyl-atrazine, hydroxy-terbuthylazine, and desethyl-terbuthylazine. Although s-triazines are used worldwide as herbicides for agricultural and nonagricultural purposes, there is limited information on the environmental impact of TER degradation products. The proposed method includes a solid-phase extraction procedure (using MCX cartridges) with adequate recovery efficiency (70-80%). The statistical evaluation of the method reveals good linearity, accuracy, and precision for the compounds determined, with RSD values less than 14.6%, while the detection limit was found to be 0.05 microg L(-1) for DIHA and 0.01 microg L(-1) for the other substances. This method can be employed in biodegradation studies of TER and its metabolites in water samples from constructed wetlands, thus assisting the evaluation of their environmental impact.  相似文献   

6.
滩涂土壤有机质含量的反射光谱估算   总被引:5,自引:0,他引:5  
Rapid determination of soil organic matter (SOM) using regression models based on soil reflectance spectral data serves an important function in precision agriculture. “deviation of arch”(DOA)-based regression and partial least squares regression (PLSR) are two popular modeling approaches to predict SOM. However, few studies have explored the accuracy of the DOA-based regression and PLSR models. Therefore, the DOA-based regression and PLSR were applied to the visible near-infrared (VNIR) spectra to estimate SOM content in the case of various dataset divisions. A two-fold cross-validation scheme was adopted and repeated 10 000 times for rigorous evaluation of the DOA-based models in comparison with the widely used PLSR model. Soil samples were collected for SOM analysis in the coastal area of northern Jiangsu Province, China. The results indicated that both modelling methods provided reasonable estimates of SOM, with PLSR outperforming DOA-based regression in general. However, the performance of PLSR for the validation dataset decreased more noticeably. Among the four DOA-based models, the linear model of the DOA provided the best estimation of SOM and a cutoff of SOM content (19.76 g kg-1), and the performance for calibration and validation datasets was consistent. As the SOM content exceeded 19.76 g kg-1, SOM became more effective in masking the spectral features of other soil properties to a certain extent. This work confirmed that reflectance spectroscopy combined with PLSR could serve as a non-destructive and cost-efficient way for rapid determination of SOM when hyperspectral data were available. The DOA-based model, which requires only 3 bands in the visible spectra, also provided SOM estimation with acceptable accuracy.  相似文献   

7.
Diffuse reflectance spectroscopy using visible (vis), near‐infrared (NIR) and mid‐infrared (mid‐IR) energy can be a powerful tool to assess and monitor soil quality and function. Mathematical pre‐processing techniques and multivariate calibrations are commonly used to develop spectroscopic models to predict soil properties. These models contain many predictor variables that are collinear and redundant by nature. Partial least squares regression (PLSR) is often used for their analysis. Wavelets can be used to smooth signals and to reduce large data sets to parsimonious representations for more efficient data storage, computation and transmission. Our aim was to investigate their potential for the analyses of soil diffuse reflectance spectra. Specifically we wished to: (i) show how wavelets can be used to represent the multi‐scale nature of soil diffuse reflectance spectra, (ii) produce parsimonious representations of the spectra using selected wavelet coefficients and (iii) improve the regression analysis for prediction of soil organic carbon (SOC) and clay content. We decomposed soil vis‐NIR and mid‐IR spectra using the discrete wavelet transform (DWT) using a Daubechies’s wavelet with two vanishing moments. A multiresolution analysis (MRA) revealed their multi‐scale nature. The MRA identified local features in the spectra that contain information on soil composition. We illustrated a technique for the selection of wavelet coefficients, which were used to produce parsimonious multivariate calibrations for SOC and clay content. Both vis‐NIR and mid‐IR data were reduced to less than 7% of their original size. The selected coefficients were also back‐transformed. Multivariate calibrations were performed by PLSR, multiple linear regression (MLR) and MLR with quadratic polynomials (MLR‐QP) using the spectra, all wavelet coefficients, the selected coefficients and their back transformations. Calibrations by MLR‐QP using the selected wavelet coefficients produced the best predictions of SOC and clay content. MLR‐QP accounted for any nonlinearity in the data. Transforming soil spectra into the wavelet domain and producing a smaller representation of the data improved the efficiency of the calibrations. The models were computed with reduced, parsimonious data sets using simpler regressions.  相似文献   

8.
基于三波长漫反射理论的玉米叶绿素含量无损测定   总被引:1,自引:0,他引:1  
传统的叶绿素无损检测方法采用是的光学透射法,它的测量值是一个相对值。植物叶片的厚度显著的影响了测量的准确性。该文利用三波长漫反射的技术设计了一个试验装置。选取了3种玉米品种,郑单-958,浚单-20和豫单-606来验证检测装置的可行性。在玉米叶片漫反射函数值和叶片叶绿素真实值之间建立了回归分析,郑单-958,浚单-20和豫单-606的决定系数R2分别为0.9766,0.9612和0.9409。试验结果证明了三波长漫反射光谱的方法测量玉米整体叶绿素含量的可行性。  相似文献   

9.
Soil degradation processes have dramatically increased in their extent and intensity over the last decades. Progressively, actions have been taken in order to evaluate and reduce the major threats that have already wreaked havoc on soil conditions. Efficient and standardized monitoring of soil conditions is thus required but soil quality research is facing an important technological challenge because of the number of properties involved in soil quality. The objective of the present review is to examine critically the suitability of near-infrared reflectance spectroscopy (NIRS) as a tool for soil quality assessment. We first detail the soil quality-related parameters (chemical, physical and biological) that can be predicted with NIRS through laboratory measurements. The ability of imaging NIRS (airborne or satellite) for mapping a minimum data set of soil quality is also discussed. Then we review the most recent research using soil reflectance spectra as an integrated measure of soil quality, from global site classification to the prediction of specific soil quality indices. We conclude that imaging NIRS enables the direct mapping of some soil properties and soil threats, but that further developments to solve several technological limitations identified are needed before it can be used for soil quality assessment. The robustness of laboratory NIRS for soil quality assessment allows its implementation in soil monitoring networks. However, its routine use requires the development of international soil spectral libraries that should become a priority for soil quality research.  相似文献   

10.
The persistence of two herbicides, simazine and terbuthylazine, and appearance of their principal dealkylated chloro-s-triazine metabolites have been studied in agricultural soil after the addition of urban sewage sludge as organic amendment. Both herbicides and metabolites were monitored during long-term laboratory incubation (140 days) and analyzed by gas chromatography with a nitrogen-phosphorus detector (GC-NPD). Residues were confirmed by gas chromatography with a mass selective detector (GC-MSD). A sonication microextraction method was used to extract the compounds. The organic amendments used were urban sewage sludge and the humic fraction of this sludge, to increase the organic matter content of the soil from 1% to 2%. For both compounds, simazine and terbuthylazine, the degradation began earlier in the amended soils. Simazine showed a higher dissipation rate than terbuthylazine, the percentage of the former at the end of the experiment being lower than 2% in all cases, while for terbuthylazine the corresponding percentage ranged from 5% to 46%. Organic amendment, mainly its humic fraction, caused a certain stabilization of terbuthylazine in the soil, but did not greatly influence the residual amount of simazine at the end of the experiment. The periodic aeration of the soil caused a greater degradation in the case of terbuthylazine. Only mono-deethylsimazine and deethylterbuthylazine were isolated from the soil during the time the experiment lasted, while the di-deethylated metabolite of simazine was not found.  相似文献   

11.
Abstract

In Western Australia soil samples to measure soil‐test phosphorus (P) are collected November to March when soils are usually dry. Most of the soils are hard‐setting when dry and it is difficult to penetrate and collect soil samples to 10 cm using the traditional sampler, which is a 2.5 cm diameter tube that is pushed into the soil by foot. Farmers collect too few soil samples at shallow depths to measure soil‐test P. In Part 1 of this paper, it was shown that soil‐test P can vary markedly for individual soil samples collected from uniform areas in paddocks. Consequently, an adequate number of soil samples needs to be collected and bulked from defined areas to measure soil‐test P. Phopshorus accumulates near the top of the soil of (i) pastures since P fertilisers are applied to the surface of pastures, and (ii) crops sown by minimum (conservation) tillage. Crops are increasingly being sown using conservation tillage methods. Collecting too few soil samples to a shallow depth can result in large errors when estimating the current P status of soils and determining optimum fertiliser application levels. A new rotating blade method of collecting soil samples to the standard 10 cm depth for measuring soil‐test P was compared with the traditional sampler. The new sampler successfully penetrated hard‐setting soils to the standard depth and the soil samples it collected produced similar soil‐test P as the traditional sampler. Consequently, the same soil‐test P calibrations determined using the traditional sampler can also be used for the new sampler. The new sampler is, therefore, recommended for collecting soil samples to measure soil‐test P. It should have application wherever hard‐setting soils pose a problem for soil P testing.  相似文献   

12.
A capillary electrophoresis-based competitive immunoassay (CEIA) with a laser-induced fluorescence (LIF) detector for the determination of carbaryl was developed. The method was based on the competitive reactions between fluorescently labeled carbaryl tracer (Ag*) and free carbaryl (Ag) with a limited amount of anticarbaryl antibody (Ab), and the relative amounts of each were separated and determined by capillary electrophoresis (CE) with an LIF detector. Using CEIA, equilibrium was reached in 30 min, and the analytical results were obtained within a further 8 min. The linear range and the detection limit for carbaryl were 0.16-50 ng/mL and 0.05 ng/mL, respectively. The sensitivity of this CEIA with an LIF detector was almost 14 times greater than that of ELISA, which used the same immuno-reagents. The method was also applied to the analysis of carbaryl in rice with rapid and simple sample pretreatment. The method is thus proposed as a fast and sensitive assay for the detection of carbaryl.  相似文献   

13.
A rapid immunochromatographic lateral-flow test strip was developed in the competitive reaction format for the detection of sulfonamides in eggs and chicken muscle. A monoclonal antibody against the common structure of sulfonamides was conjugated to colloidal gold particles as the detection reagent and an N-sulfanilyl-4-aminobenzoic acid (SUL)-bovine serum albumin (BSA) conjugate was immobilized to a nitrocellulose membrane as the capture reagent to prepare the test strip. With this method, it required only 15 min to accomplish the semiquantitative or quantitative detection of sulfonamides. The sensitivity to sulfonamides (sulfamonomethoxine, sulfamethoxydiazine, sulfadimethoxine, and sulfadiazine) was at least 10 ng/mL, as determined with an optical density scanner. By eye measurement, the sensitivity was 20 ng/mL for sulfamonomethoxine, sulfamethoxydiazine, and sulfadimethoxine and 40 ng/mL for sulfadiazine. On the basis of a sulfamonomethoxine standard curve, recoveries were from 89.5 to 95.6% for sulfamonomethoxine, from 89.5 to 95.1% for sulfamethoxydiazine, from 85.0 to 95.6% for sulfadimethoxine, and from 44.8 to 60.9% for sulfadiazine in egg and chicken muscle samples. A parallel analysis of 27 egg samples and 28 chicken muscle samples from the animal experiment showed that the differences between test strips and high-performance liquid chromatography (HPLC) were from 0.8 to 11.2% for egg samples and from 2.2 to 34% for chicken muscle samples for the quantitative detection, and the agreement rates between test strips and HPLC were 100%, based on the maximum allowed residue level of sulfadiazine (100 ng/g) established by the European Union and China. In conclusion, the method is rapid and accurate for the detection of sulfonamides in eggs and chicken muscles.  相似文献   

14.
黑土土壤水分反射光谱特征定量分析与预测   总被引:3,自引:0,他引:3  
选择单一土类黑土作为研究对象, 并准确调配其不同含水量,实验室测定土壤高光谱反射率,利用光谱分析与统计方法,定量描述了不同含水量黑土反射光谱特征,并建立了黑土含水量反射光谱预测模型,结果表明,随土壤含水量的增加,达到一定阈值(300 g kg-1),反射率存在过饱和现象,但其倒数对数微分可以有效去除饱和问题;土壤反射率倒数对数微分对土壤含水量的响应表现出三个变化阶段,导致1 870 nm波段的倒数对数微分也表现为非线性变化,需要利用分段函数进行土壤含水量的光谱精确速测。  相似文献   

15.
Near-infrared (NIR) spectroscopy is a practical spectroscopic procedure for the detection of organic compounds in matter. It is particularly useful because of its nondestructiveness, accuracy, rapid response, and easy operation. This work assesses the applicability of NIR for the rapid identification of micotoxigenic fungi and their toxic metabolites produced in naturally and artificially contaminated products. Two hundred and eighty maize samples were collected both from naturally contaminated maize crops grown in 16 areas in north-central Italy and from ears artificially inoculated with Fusarium verticillioides. All samples were analyzed for fungi infection, ergosterol, and fumonisin B1 content. The results obtained indicated that NIR could accurately predict the incidence of kernels infected by fungi, and by F. verticillioides in particular, as well as the quantity of ergosterol and fumonisin B1 in the meal. The statistics of the calibration and of the cross-validation for mold infection and for ergosterol and fumonisin B1 contents were significant. The best predictive ability for the percentage of global fungal infection and F. verticillioides was obtained using a calibration model utilizing maize kernels (r2 = 0.75 and SECV = 7.43) and maize meals (r2 = 0.79 and SECV = 10.95), respectively. This predictive performance was confirmed by the scatter plot of measured F. verticillioides infection versus NIR-predicted values in maize kernel samples (r2 = 0.80). The NIR methodology can be applied for monitoring mold contamination in postharvest maize, in particular F. verticilliodes and fumonisin presence, to distinguish contaminated lots from clean ones, and to avoid cross-contamination with other material during storage and may become a powerful tool for monitoring the safety of the food supply.  相似文献   

16.
近红外光谱法快速测定土壤碱解氮、速效磷和速效钾含量   总被引:18,自引:2,他引:18  
运用偏最小二乘法(PLS)和人工神经网络(ANN)方法分别建立了0.9 mm筛分风干黑土土壤碱解氮、速效磷和速效钾含量预测的近红外光谱(NIRS)分析模型。使用偏最小二乘算法建立的碱解氮、速效磷和速效钾校正模型的决定系数R2分别为0.9520、0.8714和0.7300,平均相对误差分别为3.42%、13.40%和7.40%。人工神经网络方法建立的碱解氮、速效磷和速效钾校正模型的决定系数分别为0.9563、0.9493和0.9522,相对误差分别为2.67%、6.48%和2.27%,测试集仿真的相对误差分别为5.44%、16.65%和7.87%。结果表明,人工神经网络方法所建立的校正模型均优于偏最小二乘法所建模型;用近红外光谱分析法预测土壤碱解氮含量是可行的,而速效磷、速效钾模型的测试集样品仿真的相对误差较大,其预测可行性还需做进一步研究。  相似文献   

17.

Background, aim, and scope  

Terbuthylazine is one of the most commonly used herbicides for vegetation management in forest plantations in New Zealand. Knowledge about the sorption of terbuthylazine on forest soils, especially the influence of coexisting organic amendments, remains obscure. In this study, we evaluated the effects of biosolids and biochars on the sorption of terbuthylazine to forest soils.  相似文献   

18.
Abstract

Weight variability of volumetric soil test samples taken with the standard 4.25 cm3 Urbana Laboratories’ soil scoop was studied as a factor influencing soil test accuracy. Also examined was effect of degree of sample pulverization on volume—weight, differences among technicians, and volume‐weight differences between dry and moist samples.

The results revealed that volumetric sampling of Maine soils can lead to major errors, particularly when quick tests are used to quantify lime requirement, extractable H, exchangeable cations, CEC, and percent base saturation. Good predictions of these properties from quick soil test results, however, are possible when samples are weighed. In the Maine Laboratory poor quantitative results from volumetric samples originated from wide variations in volume‐weight among soils. This variability was traced to degree of aggregation, which in turn was traceable to differences in texture and organic matter among samples. Technician differences were not an important source of volume‐weight variability. Volume‐weights were much lower with moist than with dried soil, but volume‐weight variability among soils was less when sampled moist.  相似文献   

19.
运用 Image J 软件分析土壤结构特征   总被引:12,自引:0,他引:12  
毕利东  张斌  潘继花 《土壤》2009,41(4):654-658
以土壤团聚体、土壤裂隙以及土壤优先流特征数码图像为研究对象,介绍了Image J软件在土壤结构特征分析中的应用.研究结果表明:①马尾松林地表层土壤团聚体圆度大于母质层土壤颗粒的圆度;②运用图像测量方法能够快速地测定土壤裂隙几何特征以及土壤收缩曲线;③土壤优先流示踪图像分析结果显示红壤性水稻土犁底层具有显著的防渗功能,而同一土壤剖面内土壤连通性孔隙存在较大的空间分异.最后,本文还对以上研究结果和图像分析方法进行了探讨.  相似文献   

20.
北京典型耕作土壤养分的近红外光谱分析   总被引:5,自引:2,他引:5  
为研究土壤养分含量分布信息,以从北京郊区一块试验田采集的72个土壤样品为试验材料,应用傅里叶变换近红外光谱技术分析了土样的全氮、全钾、有机质养分含量和pH值。采用偏最小二乘法(PLS)对光谱数据与土壤养分实测值进行回归分析,建立预测模型,以模型决定系数(R2)、校正标准差(RMSECV)、预测标准差(RMSEP)和相对分析误差(RPD)作为模型精度的评价指标。结果表明,利用该模型与光谱数据对土壤全氮、全钾、有机质养分含量和pH值进行预测,结果与实测数据具有较好的一致性,最高决定系数R2达到0.9544。偏最小二乘回归方法建立的养分预测模型能准确地对北京地区褐土土质全氮、有机质、全钾和pH值4种养分进行预测。  相似文献   

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