共查询到20条相似文献,搜索用时 78 毫秒
1.
2.
为了发掘更多玉米C型不育胞质的强恢复系资源,本研究对2份自交系Z16和7250-14-1进行了恢复能力的测定、恢复基因的遗传分析及恢复基因的分子标记定位。结果表明, Z16和7250-14-1对C黄早四、C478、C698-3和CMo17均表现为育性恢复,而对C48-2则均表现为育性部分恢复。通过对玉米CMS-C不同亚组胞质测交鉴定发现, Z16对G48-2、EC48-2、ES48-2、RB48-2及类48-2均表现为不育性保持,而7250-14-1对G48-2、EC48-2、ES48-2表现为育性部分恢复,对RB48-2和类48-2则表现为不育性保持。Z16和7250-14-1对CMS-T不育系均表现为不育性保持,而对CMS-S不育系则均表现为育性部分恢复。遗传分析显示, Z16对C478和C黄早四的育性恢复均受1对基因控制;而7250-14-1对C黄早四及C478的育性恢复分别受1对基因及2对基因控制。利用(C黄早四×Z16)F2、(C黄早四×7250-14-1)F2群体分别对恢复基因进行分子标记定位,其中Z16的恢复基因被定位于标记B-1至第8染色体短臂末端区域,物理距离为494 kb; 7250-14-1的恢复基因被定位于第8染色体短臂的标记B-1和Chr8-86080之间,物理距离为249kb。该研究不仅为玉米CMS-C"三系"配套的生产利用提供了恢复基因资源,也为玉米CMS-C恢复基因的克隆及恢复机制的研究奠定了一定基础。 相似文献
3.
向日葵CMS恢保关系的分子遗传学研究 总被引:3,自引:0,他引:3
本研究对具有代表性的向日葵CMS品系和自交系进行了遗传学及分子生物学研究。通过分子杂交分析了来自21种CMS品系的5种具代表性的不同细胞质基因类型,发现在atpA基因位点有4种分子杂交类型,根据不同的CMS类型对20种自交系的恢保关系进行了分析;并对11种自交系的恢复基因类型进行了研究,其中6个系含有Rf1基因,5个系不含有R 相似文献
4.
模拟干旱对不同耐性玉米自交系幼苗根系和水分利用效率的影响 总被引:1,自引:0,他引:1
以耐旱玉米自交系昌7-2(T)和旱敏感玉米自交系Mo17(S)为试验材料,利用4个水平PEG-6000浓度处理,研究苗期干旱对不同耐性玉米自交系根系形态特性和叶片水分利用效率的影响。结果表明:PEG-6000胁迫下,玉米自交系的根总长、根系表面积、根系体积与根系吸水能力均减小,Mo17的变化幅度大于昌7-2;植株水分利用效率增加,Mo17增加幅度小于昌7-2。与旱敏感玉米自交系相比,耐旱玉米自交系在干旱胁迫下仍能保持较正常的根系形态、较高的吸水能力和水分利用效率。 相似文献
5.
结球甘蓝细胞质雄性不育(CMS)类型的分子鉴定 总被引:4,自引:0,他引:4
本研究以高代回交转育获得的6个不育率和不育度均达100%且遗传稳定的结球甘蓝胞质雄性不育系为材料,通过对orf138与orf222基因的特异扩增发现,所鉴定的24个株系64个单株结球甘蓝CMS植株中59个单株既含有orf138基因,也有orf222基因.两种基因共存的结果暗示,该类CMS可能是ogura和nap两种类型的杂合体.进一步的序列分析结果表明,6个结球甘蓝CMS中所扩增获得的orf138基因与来自萝卜CMS的orf138基因序列完全相同;而除Bo134-2 CMS的orf222基因与甘蓝型油菜CMS orf222基因相比对有2个核苷酸差异外,其余5个CMS的orf222基因与甘蓝型油菜nap CMS orf222基因的相似性均为100%.其中,Bo134-2 CMS的orf222基因第191 bp的变异导致第64位氨基酸由精氨酸(R)突变成了赖氨酸(K). 相似文献
6.
7.
8.
为利用分子标记辅助选择加快糯玉米种质资源改良进程,以糯玉米自交系云539和普通玉米自交系昌7-2为材料,通过DNA序列测定和分析,探明糯玉米自交系云539 wx基因属于wx-D7突变类型。根据2个自交系基因组序列差异,开发出waxy基因功能标记FMD7,并通过了表型验证和通用性验证。利用开发的基因功能标记FMD7进行分子标记辅助选择,结合产量、株型和品质性状分析,获得携带wx基因的优良昌7-2改良系6个。利用本研究开发的基因功能标记FMD7进行辅助选择可加快回交转育进程,提高育种效率,为选育高产糯玉米杂交种提供材料基础,同时也为分子标记辅助选择单基因或少数主效基因控制的性状提供理论参考。 相似文献
9.
10.
11.
介绍了运用冷冻干燥技术进行速溶蛋花汤的加工,研究了生产工艺、特点以及影响产品质量的主要因素。试验表明,采用冷冻干燥技术加工的速溶蛋花汤,具有营养丰富、风味独特、方便快捷等特点。 相似文献
12.
DENG Zhao-jing 《保鲜与加工》2005,(1):153-156
A new definition of inertia,i.e. the momentum is the measuring of inertia of matter, is proposed. The article through and etc., subjects, the correctness of the new notion of inertia:The monentum is the measuring of inertia has been demonstrated, and the unsolved contradictions of the traditional definition of inetia. And the mass is the measuring of inertia has been revealed. 相似文献
13.
14.
香蕉是热带亚热带发展中国家重要的粮食作物和碳水化合物来源。但近年来,香蕉生产受到严重的病虫危害。大多数香蕉栽培品种是三倍体,生长周期长,而且不孕。由于没有种子,给繁殖和育种带来一定的困难。遗传转化技术的发展为香蕉品种的改良提供了一种有效的手段。香蕉的遗传转化方法有电激法、基因枪法、农杆菌介导法等。农杆菌介导法的应用是香蕉品种改良的一个重大突破。香蕉遗传转化的外植体也发展到多种,有原生质体,胚性细胞悬浮系,分生组织,以及横切薄片等。近几年,随着分子生物学的发展,出现了转化效率更高,重复性更好的香蕉遗传转化技术。如农杆菌和基因枪结合法,离心辅助农杆菌介导法、真空渗透技术等。这些新技术新方法的出现,必将推动香蕉产业高速发展。 相似文献
15.
基于RS与GIS技术,利用景观生态学理论及景观格局指数对布尔津县土地利用现状进行定量分析。结果显示为:(1)布尔津县以草地、林地为主要景观类型,镶嵌着耕地、水域等景观类型。(2)四个分区(即高山区、丘陵区、平原区、荒漠区)的林地资源组成差异明显,耕地、居民、交通及工矿用地集中分布在丘陵区和平原区(3)从各项指数来看,高山区的多样性指数较低;平原区是受人文因素最多的区域,景观优势度低,各种土地利用类型比例差异不大,有利于生态可持续发展,但要合理的开发利用;荒漠区生态环境相对脆弱,要加强生态环境的保护与改善。通过该研究对布尔津县的土地利用现状作一个了解,为该县乃至整个地区的景观规划和可持续发展战略提供科学依据。 相似文献
16.
17.
18.
Efficiency of water use of early plantings of sunflower 总被引:4,自引:0,他引:4
M. Auxiliadora Soriano Francisco Orgaz Francisco J. Villalobos Elías Fereres 《European Journal of Agronomy》2004,21(4):465-476
Rain fed crop production in Mediterranean environments depends to a large extent on strategies that avoid the intense summer drought. Early plantings of sunflower have given consistently higher yields in such environments, but the basis for such yield increases has not been explored. We conducted two field experiments at Cordoba (Spain) to investigate the effects of an early and a late planting date on the components of water-limited crop productivity; namely, water use (T), water use efficiency (TE) and harvest index (HI) of sunflower. The results were generalized by simulating rain fed sunflower yields, under early (1 January) and late (15 March) plantings, for a 25-year period with the aid of a simulation model of the Ceres type (OILCROP-SUN) which has been validated in Cordoba. Experimental seed yields of early plantings in 1989 and 1996 were 2.0 and 3.0 t ha−1, while late plantings yields were 1.3 and 2.4 t ha−1, for the 2 years. Average simulated yields were 2.7 ± 1.1 and 1.9 ± 0.7 t ha−1 for early and late plantings, respectively. For the 2 years, T of early plantings was higher than that of late plantings, but the response of TE and HI to planting date was not the same in the two experiments. In the simulation exercise, T and TE of early plantings were consistently higher than those of late plantings, while there were no differences in the HI for the two planting dates. We conclude that early plantings of sunflower increase rain fed yields by increasing both T and TE, while the impact of planting date on HI very much depends on the crop water stress pattern, which is quite variable from year to year even in the predictable Mediterranean environment. 相似文献
19.
The cotyledon and hypocotyl of eight varieties of alfalfa( Medicago sativa L. )are used to study the effect of different cultivation procedures and genotypes on embryogenic callus and embryoid induction,plant development and transplant of alfalfa.The varieties of alfalfa with high embryoid induction rate and high differentiation rate are screened. A high frequency regeneration system for alfalfa tissue culture is established:which is benifical to the gene transformation of alfalfa. 相似文献
20.
Automobile comfort dynamic simulation is necessary when we predict and evaluate the comfort of a automobile or optimize performance of automobile. The nine DOF vibration model of automobile is established , which acts root mean square value of body in multiple work conditions as comfort evaluation indices. Based on the comfort simulation, the Hongyan 1160 is predicted in the way of comfort. The programmes can be used in many different automobiles . The computational result indicates that the model can simulate vibration of automobile truly. It is significant to research automobile comfort evaluation to optimize performance of automobile and comfort simulation .The computational program can be applied to optimize or simulate which has some reference value. 相似文献