VA菌根对土壤酸度的耐性

The ammonification,nitrifcation and denitrification in maize rhizosphere of alluvial sols were compared with those in the bulk soil after exposure to different kinds of heavy metals.The addition of cadmium at low levels (2 mg kg^-1 and 5 mg kg^-1）could stimulate the ammonification and nitrification in the soils,while inhibition influences were found at high levels of Cd addition (10 mg kg^-1 and 20mg kg^-1），The relationship between microbial activity and cadmium concentration varied with the kind of microorganisms.The nitrifying bacteria were more sensitive to cadmium pollution than the ammonifying bacteria.When Cd( Ⅱ），Cu（Ⅱ）and Cr（Ⅵ）wer compared at the same addition concentration of 20 mg kg^-1 soil,Cd(Ⅱ） was the most effective inhibitor of ammonification and denitrification. among the three investigated heavy metals,and Cr（Ⅵ）had the most strong inhibitory influence on the nitrifying bacteria.The microbial activities in rhizosphere were higher than those in the bulk soil for most of the treatments.Cr(Ⅵ)proved to be the most effective in enhacing the microbial activities in rhizosphere,and this could be caused by the poitive reduction of Cr(Ⅵ）to Cr(Ⅲ）in rhizosphere,and the relatively sufficient existence of organic matter which intensified the adsorption of the metal,It seemed that the rhizosphere had some mitigation effect on heavy metal toxicity.     

水稻根际和周围土壤中微生物功能基因丰度与碳、氮状况及其通量之间的关系

Rapid nitrogen（N） transformations and losses occur in the rice rhizosphere through root uptake and microbial activities. However,the relationships between rice roots and rhizosphere microbes for N utilization are still unclear. We analyzed different N forms（NH＋4,NO-3, and dissolved organic N）, microbial biomass N and C, dissolved organic C, CH4 and N2O emissions, and abundance of microbial functional genes in both rhizosphere and bulk soils after 37-d rice growth in a greenhouse pot experiment. Results showed that the dissolved organic C was significantly higher in the rhizosphere soil than in the non-rhizosphere bulk soil, but microbial biomass C showed no significant difference. The concentrations of NH＋4, dissolved organic N, and microbial biomass N in the rhizosphere soil were significantly lower than those of the bulk soil, whereas NO-3in the rhizosphere soil was comparable to that in the bulk soil. The CH4 and N2O fluxes from the rhizosphere soil were much higher than those from the bulk soil. Real-time polymerase chain reaction analysis showed that the abundance of seven selected genes, bacterial and archaeal 16 S rRNA genes, amoA genes of ammonia-oxidizing archaea and ammonia-oxidizing bacteria, nosZ gene, mcrA gene, and pmoA gene, was lower in the rhizosphere soil than in the bulk soil, which is contrary to the results of previous studies. The lower concentration of N in the rhizosphere soil indicated that the competition for N in the rhizosphere soil was very strong, thus having a negative effect on the numbers of microbes. We concluded that when N was limiting, the growth of rhizosphere microorganisms depended on their competitive abilities with rice roots for N.     

外源铬对小白菜(Brassica chinensis L.)根际土壤微生物生物量和磷脂脂肪酸的剖面分布特征的影响

The effects of root activity on microbial response to cadmium （Cd） loading in the rhizosphere are not well understood. A pot experiment in greenhouse was conducted to investigate the effects of low Cd loading and root activity on microbial biomass and community structure in the rhizosphere of pakchoi （Brassica chinensis L.） on silty clay loam and silt loamy soil. Cd was added into soil as Cd（NO3）2 to reach concentrations ranging from 0.00 to 7.00 mg kg-1. The microbial biomass carbon （MBC） and community structure were affected by Cd concentration, root activity, and soil type. Lower Cd loading rates （〈 1.00 mg kg-1） stimulated the growth of pakchoi and microorganisms, but higher Cd concentrations inhibited the growth of microorganisms. The content of phospholipid fatty acids （PLFAs） was sensitive to increased Cd levels. MBC was linearly correlated with the total PLFAs. The content of general PLFAs in the fungi was positively correlated with the available Cd in the soil, whereas those in the bacteria and actinomycetes were negatively correlated with the available Cd in the soil. These results indicated that fungi were more resistant to Cd stress than bacteria or actinomycetes, and the latter was the most sensitive to Cd stress. Microbial biomass was more abundant in the rhizosphere than in the bulk soil. Root activity enhanced the growth of microorganisms and stabilized the microbial community structure in the rhizosphere. PLFA analysis was proven to be sensitive in detecting changes in the soil microbial community in response to Cd stress and root activity.     

黑土区大豆基因型的根际细菌群落结构时空动态变化

The dynamics of rhizosphere microbial communities is important for plant health and productivity, and can be influenced by soil type, plant species or genotype, and plant growth stage. A pot experiment was carried out to examine the dynamics of microbial communities in the rhizosphere of two soybean genotypes grown in a black soil in Northeast China with a long history of soybean cultivation. The two soybean genotypes, Beifeng 11 and Hai 9731, differing in productivity were grown in a mixture of black soil and siliceous sand. The bacterial communities were compared at three zone locations including rhizoplane, rhizosphere, and bulk soil at the third node (V3), early flowering (R1), and early pod (R3) stages using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rDNA. The results of principal component analyses (PCA) showed that the bacterial community structure changed with growth stage. Spatially, the bacterial communities in the rhizoplane and rhizosphere were significantly different from those in the bulk soil. Nevertheless, the bacterial communities in the rhizoplane were distinct from those in the rhizosphere at the V3 stage, while no obvious differences were found at the R1 and R3 stages. For the two genotypes, the bacterial community structure was similar at the V3 stage, but differed at the R1 and R3 stages. In other words, some bacterial populations became dominant and some others recessive at the two later stages, which contributed to the variation of the bacterial community between the two genotypes. These results suggest that soybean plants can modify the rhizosphere bacterial communities in the black soil, and there existed genotype-specific bacterial populations in the rhizosphere, which may be related to soybean productivity.     

大豆生长期间的土壤呼吸

Soil respiration induced by soybean cultivation over its entire growing season and the factors influencing soil respiration were investigated to examine the seasonal pattern of soil respiration induced by soybean cultivation, explore soybean growth and photosynthesis on soil respiration, and determine the temperature dependence on soil respiration. Soil respiration in a pot experiment with and without soybean plants was sampled using the static chamber method and measured using gas chromatograph. Air temperature was a dominant factor controlling soil respiration rate in unplanted soil. Additionally, rhizosphere respiration comprised 62% to 98% of the soil respiration rate in the soybean-planted soil varying with the soybean growth stages. Harvesting aerial parts of soybean plant caused an immediate drop in the soil respiration rate at that stage. After harvesting the aerial parts of the soybean plant, a highly significant correlation between soil respiration rate and air temperature was found at the flowering stage （P 〈 0.01）, the pod stage （P 〈 0.01）, and the seed-filling stage （P 〈 0.05）. Thus, rhizosphere respiration during the soybean-growing period not only made a great contribution to soil respiration, but also determined the seasonal variation pattern of the soll respiration rate.     

Phenological Stage, Plant Biomass, and Drought Stress Affect Microbial Biomass and Enzyme Activities in the Rhizosphere of Enteropogon macrostachyus

Indigenous grasses have been effectively used to rehabilitate degraded African drylands. Despite their success, studies examining their effects on soil bioindicators such as microbial biomass carbon(C) and enzyme activities are scarce. This study elucidates the effects of drought stress and phenological stages of a typical indigenous African grass, Enteropogon macrostachyus, on microbial biomass and enzyme activities(β-glucosidase, cellobiohydrolase, and chitinase) in the rhizosphere soil. Enteropogon macrostachyus was grown under controlled conditions. Drought stress(partial watering) was simulated during the last 10 d of plant growth, and data were compared with those from optimum moisture conditions. The rhizosphere soil was sampled after 40 d(seedling stage), 70 d(elongation stage), and 80 d(simulated drought stress). A high root:shoot ratio at seedling stage compared with elongation and reproduction stages demonstrated that E. macrostachyus invested more on root biomass in early development, to maximise the uptake of nutrients and water. Microbial biomass and enzyme activities increased with root biomass during plant growth. Ten-day drought at reproduction stage increased the microbial biomass and enzyme activities, accompanying a decrease in binding affinity and catalytic efficiency. In conclusion, drought stress controls soil organic matter decomposition and nutrient mobilization, as well as the competition between plant and microorganisms for nutrient uptake.     

丛枝菌根防止Plantago lanceolata根际菌根土壤实际消化速率受抑制

The vast majority of herbaceous plants engage into arbuscular mycorrhizal (AM) symbioses and consideration of their mycorrhizal status should be embodied in studies of plant-microbe interactions. To establish reliable AM contrasts, however, a sterilized re-inoculation procedure is commonly adopted. It was questioned whether the specific approach is sufficient for the studies targeting the bacterial domain, specifically nitrifiers, a group of autotrophic, slow growing microbes. In a controlled experiment mycorrhizal and non-mycorrhizal Plantago lanceolata were grown up in compartmentalized pots to study the AM effect on nitrification rates in the plant rhizosphere. Nitrification rates were assayed following an extensive 3-week bacterial equilibration step of the re-inoculated soil and a 13-week plant growth period in a controlled environment. Under these specific conditions, the nitrification potential levels at harvest were exceptionally low, and actual nitrification rates of the root compartment of non-mycorrhizal P. lanceolata were significantly lower than those of any other compartment. It is then argued that the specific effects should be attributed to the alleged higher growth rates of non-mycorrhizal plants that are known to occur early in the AM experiment. It is concluded that the specific experimental approach is not suitable for the study of microbes with slow growth rates.     

棕壤线虫对尿素配施尿酶抑制剂和硝化抑制剂的响应研究

Effects of urea amended with urease and nitrification inhibitors on soil nematode communities were studied in a Hapli- Udic Argosol （Cambisol, FAO） in Liaoning Province of Northeast China. A completely random design with four treatments, i.e., conventional urea （CU）, slow-release urea amended with a liquid urease inhibitor （SRU1）, SRU1 ＋nitrification inhibitor dicyandiamide （SRU2）, and SRU1 ＋ nitrification inhibitor 3,5-dimethylpyrazole （SRU3） and four replicates were applied. Thirty-nine genera of nematodes were identified, with Cephalobus and Aphelenchus being dominant; and in all treatments, the dominant trophic group was bacterivores. In addition, during the growth period of spring wheat （Triticum aestivum L.）, soil urease activity was lower in SRUs than in CU. The numbers of total nematodes and bacterivores at wheat heading and ripening stages, and omnivores-predators at ripening stage were higher in SUR3 than in CU, SRU1 and SRU2 （P 〈 0.05）.     

Phosphorus Speciation and Nutrient Stoichiometry in the Soil-Plant System During Primary Ecological Restoration of Copper Mine Tailings

The effects of plant vegetation on phosphorus(P) speciation, pH, total carbon concentration, total nitrogen concentration, and alkaline phosphatase activities were investigated to explore the P uptake strategy of plants in low-P soil and to determine the nutrient stoichiometric ratio changes in the rhizosphere of plants(Imperata cylindrica, Miscanthus floridulus, Zoysia sinica, Artemisia lavandulaefolia, Indigofera pseudotinctoria, and Conyza canadensis) which had grown for approximately 15 years in copper mine tailings, East China. The results showed that the average pH values in the rhizosphere decreased by 0.06–1.37 compared with those in the non-rhizosphere. The alkaline phosphatase activities of the rhizosphere were significantly higher than those in the non-rhizosphere.The mean concentrations of aluminum(Al)-and iron(Fe)-bound P and Ca_2-P(CaHPO_4) in the rhizosphere of all plants were 5.4% to 87.7%, 49.2% to 214.2%, and 86.6% to 147.6% higher than those in the non-rhizosphere, respectively. Except for Ca_8-P(Ca_8H_2(PO_4)_6)and Ca_(10)-P(Ca_(10)(PO_4)_6(OH)_2) in the rhizosphere, all kinds of inorganic P forms were negatively correlated with pH. Significant correlation was also observed among the concentrations of dominant forms of inorganic P, C, and N and alkaline phosphatase activities in the rhizosphere. Among the studied species, I. pseudotinctoria showed the most significant effect on enhancing soil available P concentration. The stoichiometric ratios of C:P and N:P were apparently higher in the rhizosphere than the non-rhizosphere, whereas these ratios were far below the ratios commonly observed in Chinese soils. These results indicated that the plant growth effectively affected P fractions possibly by changing pH, C and N concentrations, and alkaline phosphatase activity, in the rhizosphere in copper mine tailings.     

土壤－水分－植物体系中铅和镉的交互作用及其机制: Ⅱ. 根际中Pb-Cd的交互作用

The interaction of Pb-Cd can be observed not only in the uptake process of elements by plants and in their influence on the growth,but also in rhizosphere.The changes in extractable Cd and Pb concentrations in the rhizosphere soil of rice plants ,root exudates from wheat and wheat plant and their complexing capacity,with Pa and Cd were investigated under different Pb and Cd treatments.Results showed that the concentration of extractable Cd in the rhizosphere of rice in red soil was markedly increased by Pb-Cd interaction,It increased by 56% in the treatment with Pb and Cd added against that in the treatment with only Cd added in soil . The considerable differences in both composition and amount of root exudate from wheat and rice were found among different treatments.Pb and Cd might be complexed by root exudates ,The concentrations of free Pb and Cd in the solution were increased markedly by adding root exudate from wheat and decreased by that from rice due to Pd-Cd interaction.The distribution patterns of Pb and Cd in roots were affected by Pb-Cd interaction,which accelerated transport of Pb into internal tissue and retarded accumulation of Cd in external tissue.     

Changes in soil biological activities under reduced soil pH during Thlaspi caerulescens phytoextraction

Phytoextraction of soil Cd and Zn may require reduction in soil pH in order to achieve high metal uptake. Reducing the pH of high metal soil, however, could negatively affect soil ecosystem function and health. The objectives of this study were to characterize the quantitative causal relationship between pH and soil biological activities in two Zn and Cd contaminated soils and to investigate the relationship between metals and soil biological activities under low pH. Soils were adjusted to five or six different pH levels by sulfur addition, followed by salt leaching. Thlaspi caerulescens was grown for 6 months, and both the rhizosphere and non-rhizosphere soil biological activities were tested after harvest. Reducing pH significantly lowered soil alkaline phosphatase activity, arylsulphatase activity, nitrification potential, and respiration. However, acid phosphatase activity was increased with decreasing pH. The relationship between soil biological activities and pH was well characterized by linear or quadratic regression models with R² values ranging from 0.57 to 0.99. In general, the three enzyme activities, nitrification potential, and the ratio of alkaline phosphatase to acid phosphatase activity were very sensitive indicators of soil pH status while soil respiration was not sensitive to pH change. The rhizosphere soil had higher biological activities than non-rhizosphere soil. The negative effects observed in the non-rhizosphere soil were alleviated by the rhizosphere influence. However, rhizosphere soil after 6 months phytoextraction showed lower nitrification potential than non-rhizosphere soil, probably due to substrate limitation in our study.     

Differences between soil solutions obtained from rhizosphere and non-rhizosphere soils by water displacement and soil centrifugation

Soil solution was obtained from potted rhizosphere or non-rhizosphere soils by water displacement or soil centrifugation. The pH of the displaced solutions was lower than that of bulk soils when solutions were obtained from non-rhizosphere soil, although it increased as plants grew. This increase probably reflected true changes in rhizosphere pH, generated by the uptake by plants of N0₃-N. In contrast, the pH of soil centrifugates was usually close to that of the bulk soils, implying that buffering by colloids had occurred during sampling. Concentrations of elements in solutions from non-rhizosphere soil were similar for both methods when soils were incubated at ambient pCO₂. However, when non-rhizosphere soils were incubated at elevated pCO₂, displacement solutions had lower pH values, and much larger concentrations of elements, compared to soil centrifugates. Comparison of mass flow of elements versus actual plant uptake showed that Ca and Mg accumulated, while K, Zn and Cd were depleted from the rhizosphere. Displacement solutions showed this accumulation or depletion of the elements more clearly than soil centrifugates. These differences were attributed to the fact that, at constant soil moisture, the rhizosphere developed mainly in larger pores, which were sampled by displacement. With centrifugation, a mixture of pore sizes was sampled, so that rhizosphere solution was only obtained when all of the soil had become rhizosphere. Soil centrifugates obtained after 22 days of growth also contained higher concentrations of organic carbon than displacement solutions, indicating contamination due to the disruption of roots and/or micro-organisms. We conclude that water displacement is suitable for sampling solution from light to medium textured rhizosphere or non-rhizosphere soils and that soil centrifugation is only of limited suitability.     

免耕和稻草还田对稻田土壤氮素转化强度的影响

为了明确土壤氮素转化对免耕和稻草还田的响应,研究了不同耕作对土壤(0～5 cm、5～12 cm和12～20 cm土层)氮素转化强度和铵态氮含量的影响。结果表明,免耕显著提高0～5 cm土层土壤氨化强度和铵态氮含量,但对于5～12 cm和12～20 cm土层趋势则相反;各土层土壤硝化势、反硝化强度免耕明显低于常耕,表明免耕促进0～5 cm土层有机氮的氨化作用和降低5～20 cm土层有机氮的氨化及0～20 cm土层土壤硝化-反硝化损失。稻草还田对土壤氮素转化强度的影响因耕作而异,免耕下,稻草还田促进5～20 cm土层土壤氨化强度及各土层反硝化强度和铵态氮含量,对0～5 cm土层土壤氨化强度和各土层土壤硝化势影响不大;常耕下,稻草还田降低5～20 cm土层铵态氮含量和硝化势,提高各土层氨化强度和反硝化强度。因而,免耕结合稻草还田更有利于土壤氮素的释放、供应,但需注意防止反硝化损失。     

影响砂姜黑土麦田土壤氮素转化的生物学因素 及其对供氮量的响应

砂姜黑土是我国典型的中低产田土壤类型,研究其在土壤微生物驱动下的氮素转化过程及其机制,可为定向调控土壤氮素转化过程,提高氮素利用效率并减少其负面效应提供科学依据。试验设置0 kg·hm~(-2)、120 kg·hm~(-2)、225 kg·hm~(-2)和330 kg·hm~(-2) 4个供氮量,分别于冬小麦越冬期、拔节期、抽穗期、开花期、灌浆期和成熟期测定小麦根际土壤氮转化相关微生物作用(氨化作用、硝化作用和反硝化作用)强度和土壤氮素转化相关酶(脲酶、蛋白酶)活性,土壤净氮素矿化速率、土壤硝态氮和铵态氮含量的变化,研究影响砂姜黑土麦田土壤氮素转化的生物学因素及其对不同供氮量的响应。结果表明,土壤氮素转化微生物及酶活跃时期为拔节到灌浆期,灌浆期之后土壤氨化作用强度、硝化作用强度、脲酶及蛋白酶活性降低;土壤净氮素矿化速率与土壤氮素转化微生物作用强度及酶活性的活跃期较为一致,在开花前后达到最高。除脲酶活性随供氮量增加持续上升外,土壤氮素转化微生物作用强度及蛋白酶活性均随供氮量的增加,在225 kg·hm~(-2)处理下达到最高,进一步增加供氮量至330 kg·hm~(-2),微生物作用强度及酶活性均表现出不同程度的下降。可见,砂姜黑土土壤氮素转化的活跃期与小麦需氮高峰期基本一致,有利于冬小麦的生长。但由于砂姜黑土中土壤硝化作用强度较低,土壤硝化能力有限,从而降低了氮素可利用性,且增加了土壤氨挥发损失的潜在风险。在一定范围内增加供氮量,有利于土壤氮素的转化,但供氮过多(330 kg·hm~(-2))则不利于砂姜黑土供氮能力的提高。     

不同年龄木麻黄林地根际土壤养分含量和酶活性动态

在福建省东山县滨海沙地，开展了不同年龄木麻黄林地根际和非根际土壤养分和酶活性的测定，研究结果表明：（1）不同年龄木麻黄林地根际pH小于非根际土壤，随林龄增长根际土壤和非根际土壤pH值均表现为下降趋势；根际土壤有机质、全氮和水解氮含量高于非根际土壤，各土层有机质含量在中龄林时最大；从中龄林阶段至过熟林，水解氮含量下降；全磷和速效磷含量从幼林发育至中龄林、近熟林逐渐减少，至过熟林略有恢复；根际土壤全钾、速效钾含量呈增加趋势。（2）不同年龄木麻黄林地根际CEC值、水解性总酸度、交换性盐基总量、交换性Mg^2＋均大于非根际土壤；幼林龄根际交换性Ca^2＋低于非根际；土壤CEC值在中龄林时最高。（3）不同年龄木麻黄林地根际土壤磷酸酶、过氧化物酶和多酚氧化酶活性均大于非根际土壤；随着林木的生长，根际和非根际土壤磷酸酶活性逐渐升高，并且根际与非根际间的差异也呈增大趋势；根际过氧化物酶活性从幼龄林到中龄林下降，随着林木生长至过熟林有所升高；根际多酚氧化酶活性在过熟林阶段高于其它发育阶段。     

红壤稻田不同生育期土壤氨氧化微生物群落结构

以福建省红壤稻田土壤为对象,通过提取土壤总DNA,利用特异引物进行PCR(聚合酶链反应)扩增和DGGE(变性梯度凝胶电泳)并结合DNA克隆测序,研究了水稻生长过程中稻田土壤氨氧化细菌和氨氧化古菌群落结构的变化。结果显示:稻田土壤具有丰富的氨氧化细菌和氨氧化古菌资源。水稻生长过程中土壤氨氧化细菌群落组成较为稳定,只表现出水稻生长前期(苗期、分蘖期)和中后期(孕穗期、成熟期)间存在一定差异。而土壤氨氧化古菌群落组成变化较大,在水稻生长的苗期、分蘖期、孕穗期和成熟期4个时期间均存在一定差异。在水稻生长过程中,土壤氨氧化细菌群落多样性指数无显著性变化,但氨氧化古菌群落多样性指数随水稻生长明显提高,孕穗期后才达到平稳。水稻生长前期土壤硝化势也具有显著上升趋势,孕穗期时达到最高,而后有所下降。土壤硝化势与氨氧化古菌群落多样性指数具有显著正相关性,与氨氧化细菌没有相关性。研究表明,氨氧化古菌对红壤稻田土壤硝化作用的影响程度较大,证实了氨氧化微生物尤其是氨氧化古菌在稻田土壤微生物组成及其生态系统功能中的重要性。     

Effects of pine roots on microorganisms,fauna, and nitrogen availability in two soil horizons of a coniferous forest spodosol

Summary We investigated the effects of pitch pine seedling roots on extractable N, microbial growth rate, biomass C and N, and nematodes and microarthropods in microcosms with either organic (41% C, 1.14% N) or mineral (0.05% C, 0.01% N) horizon soils of a spondosol. Root quantity was manipulated by varying plant density (0, 1, 2, or 4 seedlings) and rhizosphere soil was separated from non-rhizosphere soil by a 1.2 m mesh fabric. In the rhizosphere of organic soil horizons, moisture, microbial growth rate, biomass C and N, and extractable N declined as root density was increased, but there was little effect on nematodes or microarthropods. High levels of extractable N remained after 5 months, suggesting that N mineralization was stimulated during the incubation. In the rhizosphere of mineral soil horizons, microbial growth rate, and nematode and microarthropod abundances increased at higher root density, and in the absence of roots faunal abundance approached zero. Faunal activity was concentrated in the rhizosphere compared to non-rhizosphere soil. In organic soil horizons, roots may limit microbial activity by reducing soil moisture and/or N availability. However, in mineral soil horizons, where nutrient levels are very low, root inputs can stimulate microbial growth and faunal abundance by providing important substrates for microbial growth. Our results demonstrate a rhizosphere effect for soil fauna in the mineral soil, and thus extends the rhizosphere concept to components of the soil community other than microbes for forest ecosystems. Although our results need to be verified by field manipulations, we suggest that the effects of pine roots on nutrient cycling processes in coniferous forests can vary with soil nutrient content and, therefore, position in the soil profile.     

放牧对羊草草原土壤氮素循环的影响

研究了放牧对内蒙古羊草草原土壤中N素分解细菌以及固N作用、氨化作用和硝化作用的影响,结果表明,轻度放牧使土壤中4类N素转化细菌的数量显著增加,也显著加强了土壤中的固N作用、氨化作用和硝化作用。促进了土壤中的N素循环;随放牧强度的增加,重度放牧则导致草原土壤中4类N素转化细菌数量均显著降低。显著抑制土壤中的固N作用和氨化作用,会阻碍土壤中的N循环的进程。放牧强度对N素转化细菌以及N循环中的固N作用、氨化作用和硝化作用的季节性变化规律无明显影响。     

苗期干旱胁迫下施氮对玉米氮素吸收和土壤生物化学性质的影响

研究苗期干旱胁迫下施氮对东北春玉米氮素吸收利用和土壤生物化学性质的影响,为区域玉米养分管理与逆境调控提供依据.研究设置水、氮二因素盆栽试验,土壤水分包括3个水平:田间持水量的30％(W0),50％(W1)和70％(W2);施氮量包括2个水平:不施氮(N0)和施氮0.24 g/kg(N1),测定不同水氮条件下玉米苗期的植...