荒漠草原不同发育阶段柠条灌丛堆土壤真菌群落多样性特征研究

为了解荒漠草原柠条灌丛堆演化过程中环境微生物多样性变化与形成机理,以宁夏荒漠草原不同发育阶段的柠条灌丛堆为研究对象,采用高通量测序技术分析其土壤真菌的群落结构及多样性特征,探究发育阶段和土壤理化因子对土壤真菌群落结构和功能的影响因素。结果显示：获得的2662个真菌操作分类单元(Operational taxonomic unit, OTU)属于13门40纲524属,其中优势菌门为子囊菌门(Ascomycota,占77.48%～82.07%)、担子菌门(Basidiomycota,占7.42%～10.07%)以及被孢霉门(Mortierellomycota,占5.42%～10.78%);优势菌纲有粪壳菌纲(Sordariomycetes,占27.10%～36.23%)、座囊菌纲(Dothideomycetes,占25.69%～35.30%)、散囊菌纲(Eurotiomycetes,占12.64%～16.33%)、伞菌纲(Agaricomycetes,占7.07%～7.57%)。土壤有机碳、速效磷、总氮、电导率在柠条灌丛堆不同发育阶段不同部位有显著性差异,而总磷、pH无明显差异。在柠条灌丛...     

Characteristics of root pullout resistance of Caragana korshinskii Kom. in the loess area of northeastern Qinghai-Tibet Plateau,China

Roots exert pullout resistance under pullout force, allowing plants to resist uprooting. However, the pullout resistance characteristics of taproot-type shrub species of different ages remain unclear. In this study, in order to improve our knowledge of pullout resistance characteristics of taproot systems of shrub species, we selected the shrub species Caragana korshinskii Kom. in different growth periods as the research plant and conducted in situ root pullout test. The relationships among the maximum pullout resistance, peak root displacement, shrub growth period, and aboveground growth indices (plant height and plant crown breadth) were analyzed, as well as the mechanical process of uprooting. Pullout resistance of 4-15 year-old C. korshinskii ranged from 2.49 (±0.25) to 14.71 (±4.96) kN, and the peak displacement ranged from 11.77 (±8.61) to 26.50 (±16.09) cm. The maximum pullout resistance and the peak displacement of roots increased as a power function (R²=0.9038) and a linear function (R²=0.8242) with increasing age, respectively. The maximum pullout resistance and the peak displacement increased with increasing plant height; however, this relationship was not significant. The maximum pullout resistance increased exponentially (R²=0.5522) as the crown breadth increased. There was no significant relationship between the peak displacement and crown breadth. The pullout resistance and displacement curve were divided into three stages: the initial nonlinear growth, linear growth, and nonlinear stages. Two modes of failure of a single root occurred when the roots were subjected to vertical loading forces: the synchronous breakage mode and the periderm preferential breakage mode. These findings provide a foundation for further investigation of the soil reinforcement and slope protection mechanisms of this shrub species in the loess area of northeastern Qinghai-Tibet Plateau, China.     

Diversity of soil bacteria and fungi communities in artificial forests of the sandy-hilly region of Northwest China

Soil erosion is a serious issue in the sandy-hilly region of Shanxi Province, Northwest China. There has been gradual improvement due to vegetation restoration, but soil microbial community characteristics in different vegetation plantation types have not been widely investigated. To address this, we analyzed soil bacterial and fungal community structures, diversity, and microbial and soil environmental factors in Caragana korshinskii Kom., Populus tomentosa Carr., Populus simonii Carr., Salix matsudana Koidz, and Pinus tabulaeformis Carr. forests. There were no significant differences in the dominant bacterial community compositions among the five forest types. The alpha diversity of the bacteria and fungi communities showed that ACE (abundance-based coverage estimator), Chao1, and Shannon indices in C. korshinskii forest were significantly higher than those in the other four forest types (P<0.05). Soil organic matter, total nitrogen, and urease had a greater impact on bacterial community composition, while total nitrogen, β-glucosidase, and urease had a greater impact on fungal community composition. The relative abundance of beneficial and pathogenic microorganisms was similar across all forest types. Based on microbial community composition, diversity, and soil fertility, we ranked the plantations from most to least suitable as follows: C. korshinskii, S. matsudana, P. tabulaeformis, P. tomentosa, and P. simonii.     

Changes in soil carbon stocks and related soil properties along a 50-year grassland-to-cropland conversion chronosequence in an agro-pastoral ecotone of Inner Mongolia, China

Land use change significantly influences soil properties.There is little information available on the long-term effects of post-reclamation from grassland to cropland on soil properties.We compared soil carbon(C) and nitrogen(N) storage and related soil properties in a 50-year cultivation chronosequence of grassland in the agro-pastoral ecotone of Inner Mongolia.Field surveys on land use changes during the period of 1955-2002 were conducted to build a chronosequence of cropland of different ages since the conversion from grassland.The results showed that soil C and N storage,soil texture,and soil nutrient contents varied with land use types and cropland ages(P<0.01).In the 0-30 cm soil layer,the soil organic carbon(SOC) density was significantly lower in the croplands(3.28 kg C/m2 for C50 soil) than in the grasslands(6.32 kg C/m2).After 5,10,15,20,35,and 50 years of crop planting(years since the onset of cultivation),the SOC losses were 17%,12%,19%,47%,46%,and 48%,respectively,compared with the grasslands.The soil total nitrogen(TN) density of the grasslands was 65 g N/m2,and TN density of the cropland soil was 35 g N/m2 after 50 years of crop planting.Both the SOC and TN densities could be quantitatively determined by a negative exponential function of cropland age(P<0.0001,R2=0.8528;P<0.0001,R2=0.9637).The dissolved organic carbon(DOC) content,soil available potassium(AK) content,clay content,and pH value were decreased;and the soil bulk density and sand content were increased since the conversion of grassland into cropland during the 50-year period.Our results show soil nutrients were higher in grassland than in cropland.The conversion of grasslands to croplands induced a loss of soil C storage and changes of related soil properties.The reclamation time of cultivated soil(cropland age) had significant effects on soil properties in the study area.     

Arbuscular mycorrhizal fungal community structure and diversity are affected by host plant species and soil depth in the Mu Us Desert,northwest China

The vertical diversity and distribution of arbuscular mycorrhizal (AM) fungi were investigated in the Mu Us Desert, northwest China. Soils were sampled to 50?cm in depth in the rhizospheres of Hedysarum laeve, Artemisia ordosica, and Psammochloa villosa and 44 AM fungal species belonging to 10 genera were isolated. Several of these species have peculiar morphological features, which are distinct from other habitats. AM fungal diversity and distribution differed significantly among the three host plants and the five soil layers. Spore density, species richness, and the Shannon-Wiener index of AM fungi were 0.55–4.3 spores g^?1 soil, 7–36 and 1.78–2.89, respectively. Spore density and species richness had a significant positive correlation with soil total phosphorus content (0.0377–0.1129?mg?g^?1), and a negative correlation with soil pH (7.19–7.64). Nonmetric multidimensional scaling, PerMANOVA, and structural equation model analysis demonstrated that host plant species and soil depth significantly and directly influenced the structure of AM fungal communities. We concluded that diversity and distribution of AM fungi might be influenced by plant species, soil depth patterns, and soil nutrient availability in desert ecosystems. This research into AM fungal communities may lead to the development of AM fungi treatment for the mitigation of soil erosion and desertification using mycorrhizal plants, such as H. laeve, A. ordosica, and P. villosa.     

镰叶锦鸡儿湿地土壤酶活性分布特征及其与活性有机碳表征指数的关系

选取伊犁河谷昭苏县镰叶锦鸡儿湿地土壤为研究对象,研究其土壤酶活性(脲酶活性,蔗糖酶活性,过氧化氢酶活性)与土壤活性有机碳(土壤水溶性有机碳WSOC,土壤易氧化碳ROC,土壤微生物量碳MBC)的分布特征及其内在关系,分析镰叶锦鸡儿湿地土壤酶活性和土壤有机碳表征指数两者的相互关系。研究表明:镰叶锦鸡儿湿地的土壤酶活性随着土层深度的增加而下降;活性有机碳含量随土壤深度的增加,其垂直剖面的含量呈下降趋势。镰叶锦鸡儿湿地解放桥有锦鸡儿剖面的土壤酶活性和活性有机碳表征指数的含量均大于无锦鸡儿剖面,表明镰叶锦鸡儿在生长过程中可以提高土壤酶活性与活性有机碳含量;蔗糖酶,脲酶和过氧化氢酶与土壤活性有机碳呈极显著相关关系(P0.01),表明土壤酶对土壤活性有机碳的迁移转化起着重要作用。     

不同种植年限老芒麦根际土壤营养与细菌群落多样性

为揭示不同生长年限老芒麦根际土壤营养及细菌多样性变化特征,采用高通量测序技术对种植3 a(Y3)、4 a(Y4)和7 a(Y7)老芒麦根际土壤细菌群落组成和多样性进行分析。结果表明:随着种植年限的增加,土壤pH由7.95增加至8.03;土壤全氮(TN)、速效氮(AN)、速效磷(AP)含量随着老芒麦生长年限的增加显著降低,Y4较Y3分别降低了21.9%、12.0%、32.0%,Y7较Y3分别降低了34.5%、24.2%、38.9%;Chao 1和Shannon指数亦随种植年限的增加而降低,Y3、Y4和Y7的Chao 1指数分别为2605.42,2518.46和2483.89,Shannon指数分别为9.79,9.65和9.62。聚类分析表明,Y4与Y3之间细菌群落相似性基本一致,与Y7样地间差异较大。在门分类水平上,变形菌门、放线菌门、酸杆菌门为优势菌门;在属分类水平上,优势菌群依次为Norank_cSubgroup₆、鞘氨醇单胞菌属(Sphingomonas)、Norank_fGe...     

Response of C:N:P in the plant-soil system and stoichiometric homeostasis of Nitraria tangutorum leaves in the oasis-desert ecotone,Northwest China

Nitraria tangutorum nebkhas are widely distributed in the arid and semi-arid desert areas of China. The formation and development of N. tangutorum nebkhas are the result of the interaction between vegetation and the surrounding environment in the process of community succession. Different successional stages of N. tangutorum nebkhas result in differences in the community structure and composition, thereby strongly affecting the distribution of soil nutrients and ecosystem stability. However, the ecological stoichiometry of N. tangutorum nebkhas in different successional stages remains poorly understood. Understanding the stoichiometric homeostasis of N. tangutorum could provide insights into its adaptability to the arid and semi-arid desert environments. Therefore, we analyzed the stoichiometric characteristics of N. tangutorum in four successional stages, i.e., rudimental, developing, stabilizing, and degrading stages using a homeostasis model in an oasis-desert ecotone of Northwest China. The results showed that soil organic carbon (SOC), total nitrogen (TN), and total phosphorus (TP) contents and their ratios in the 0-100 cm soil depth were significantly lower than the averages at regional and global scales and were weakly influenced by successional stages in the oasis-desert ecotone. TN and TP contents and C:N:P in the soil showed similar trends. Total carbon (TC) and TN contents in leaves were 450.69-481.07 and 19.72-29.35 g/kg, respectively, indicating that leaves of N. tangutorum shrubs had a high storage capacity for C and N. Leaf TC and TN contents and N:P ratio increased from the rudimental stage to the stabilizing stage and then decreased in the degrading stage, while the reverse trend was found for leaf C:N. Leaf TP content decreased from the rudimental stage to the degrading stage and changed significantly in late successional stages. N:P ratio was above the theoretical limit of 14, indicating that the growth of N. tangutorum shrubs was limited by P during successional stages. Leaf N, P, and N:P homeostasis in four successional stages was identified as 'strictly homeostasis'. Redundancy analysis (RDA) revealed that soil acidity (pH) and the maximum water holding capacity were the main factors affecting C:N:P stoichiometric characteristics in N. tangutorum leaves. Our study demonstrated that N. tangutorum with a high degree of stoichiometric homeostasis could better cope with the arid desert environment.     

红砂植被盖度对土壤不同形态碳、氮及细菌多样性的影响

以自然恢复的红砂群落为研究对象,探讨黄土高原红砂植被不同盖度对土壤不同形态碳、氮及细菌多样性的影响,为该地区的人工生态恢复措施提供理论支撑.在兰州市南北两山植被恢复技术研究与示范基地,按照5级盖度分类法设置红砂植被盖度梯度,重点对土壤养分碳氮、微生物量碳氮和细菌多样性开展研究.结果表明:随着植被恢复,土壤有机碳 (SOC)和全氮(TN)、土壤微生物碳 (MBC)和微生物氮(MBN)逐渐提高,并且增加比较快 ,但是当总盖度达到48.73%之后,增加比较缓慢,而且增加的差异不显著.相同的植被盖度对土壤有机碳、全氮和土壤微生物碳、氮的影响趋于一致.土壤细菌多样性随植被盖度有所增加,在植被盖度达到48.73%后多样性维持在彼此接近的水平,尽管微生物多样性群落结构有差异 .在植被稀疏、物种多样性较低的干旱坡地,红砂植被盖度增加明显改善了土壤生态功能, 但是片面追求植被盖度的增加,对土壤特性改善有限.     

Competition,spatial pattern,and regeneration of Haloxylon ammodendron and Haloxylon persicum communities in the Gurbantunggut Desert,Northwest China

Competition, spatial pattern, and regeneration are important factors affecting community composition, structure, and dynamics. In this study, we surveyed 300 quadrats from three dunes(i.e., fixed dunes, semifixed dunes, and mobile dunes) in the Gurbantunggut Desert, Northwest China, from late May to early June in 2021. The intraspecific and interspecific competition, spatial pattern, and regeneration of Haloxylon ammodendron and Haloxylon persicum were studied using the Hegyi competition index a...     

Changes in diversity,composition and assembly processes of soil microbial communities during Robinia pseudoacacia L. restoration on the Loess Plateau,China

Robinia pseudoacacia L. (RP) restoration has increased vegetation cover in semi-arid regions on the Loess Plateau of China, but ecological problems have also occurred due to RP restoration, such as reduced soil moisture. Further, it is still uncertain how microbial diversity, composition and assembly processes change with RP restoration in semi-arid regions. Therefore, amplicon sequencing of small subunit ribosomal ribonucleic acid (16S rRNA) and internal transcribed spacer (ITS) genes was performed to study soil bacterial and fungal diversity, composition and assembly processes at four study sites with different stand ages of RP plantations (Y10, RP plantation with stand ages less than 10 a; Y15, RP plantation with stand ages approximately 15 a; Y25, RP plantation with stand ages approximately 25 a; and Y40, RP plantation with stand ages approximately 40 a) along a 40-a chronosequence on the Loess Plateau. The diversity of soil bacteria and fungi increased significantly during the restoration period from 10 to 15 a (P<0.05). However, compared with Y15, bacterial diversity was lower at Y25 and Y40, and fungal diversity remained stable during the restoration period between 25 and 40 a. The relative abundances of Proteobacteria and Ascomycota increased during the restoration period from 10 to 15 a. Conversely, after 15 a of restoration, they both decreased, whereas the relative abundances of Actinomycetes, Acidobacteria and Basidiomycota gradually increased. The variations in soil bacterial communities were mainly related to changes in soil total nitrogen, nitrate nitrogen and moisture contents, while soil fungal communities were mainly shaped by soil organic carbon and nitrate nitrogen contents. Bacterial communities were structured by the heterogeneous selection and stochastic process, while fungal communities were structured primarily by the stochastic process. The RP restoration induced an increase in the relative importance of heterogeneous selection on bacterial communities. Overall, this study reveals the changes in microbial diversity, community composition and assembly processes with RP restoration on the Loess Plateau and provides a new perspective on the effects of vegetation restoration on soil microbial communities in semi-arid regions.     

Severe drought strongly reduces water use and its recovery ability of mature Mongolian Scots pine (Pinus sylvestris var. mongolica Litv.) in a semi-arid sandy environment of northern China

Trees growing in a semi-arid sandy environment are often exposed to drought conditions due to seasonal variations in precipitation, low soil water retention and deep groundwater level. However, adaptability and plasticity of individuals to the changing drought conditions greatly vary among tree species. In this study, we estimated water use (T_s) of Mongolian Scots pine (MSP; Pinus sylvestris var. mongolica Litv.) based on sap flux density measurements over four successive years (2013-2016) that exhibited significant fluctuations in precipitation in a semi-arid sandy environment of northern China. The results showed that fluctuations in daily T_s synchronously varied with dry-wet cycles of soil moisture over the study period. The daily ratio of water use to reference evapotranspiration (T_s/ET₀) on sunny days in each year showed a negative linear relationship with the severity of drought in the upper soil layer (0-1 m; P<0.01). The decrease in Ts induced by erratic drought during the growing season recovered due to precipitation. However, this recovery ability failed under prolonged and severe droughts. The T_s/ET₀ ratio significantly declined with the progressive reduction in the groundwater level (g_w) over the study period (P<0.01). We concluded that the upper soil layer contributed the most to the T_s of MSP during the growing season. The severity and duration of droughts in this layer greatly reduced T_s. Nevertheless, g_w determined whether the T_s could completely recover after the alleviation of long-term soil drought. These results provide practical information for optimizing MSP management to stop ongoing degradation in the semi-arid sandy environments.