Water use characteristics of different pioneer shrubs at different ages in western Chinese Loess Plateau: Evidence from δ2H offset correction

Caragana korshinskii Kom. and Tamarix ramosissima Ledeb. are pioneer shrubs for water and soil conservation, and for windbreak and sand fixation in arid and semi-arid areas. Understanding the water use characteristics of different pioneer shrubs at different ages is of great importance for their survival when extreme rainfall occurs. In recent years, the stable isotope tracing technique has been used in exploring the water use strategies of plants. However, the widespread δ²H offsets of stem water from its potential sources result in conflicting interpretations of water utilization of plants in arid and semi-arid areas. In this study, we used three sets of hydrogen and oxygen stable isotope data (δ²H and δ¹⁸O, corrected δ²H_{_c1} based on SW-excess and δ¹⁸O, and corrected δ²H_{_c2} based on -8.1‰ and δ¹⁸O) as inputs for the MixSIAR model to explore the water use characteristics of C. korshinskii and T. ramosissima at different ages and in response to rainfall. The results showed that δ²H_{_c1} and δ¹⁸O have the best performance, and the contribution rate of deep soil water was underestimated because of δ²H offset. During the dry periods, C. korshinskii and T. ramosissima at different ages both obtained mostly water from deeper soil layers. After rainfall, the proportions of surface (0-10 cm) and shallow (10-40 cm) soil water for C. korshinskii and T. ramosissima at different ages both increased. Nevertheless, there were different response mechanisms of these two plants for rainfall. In addition, C. korshinskii absorbed various potential water sources, while T. ramosissima only used deep water. These flexible water use characteristics of C. korshinskii and T. ramosissima might facilitate the coexistence of plants once extreme rainfall occurs. Thus, reasonable allocation of different plants may be a good vegetation restoration program in western Chinese Loess Plateau.     

Succession of soil bacterial and fungal communities of Caragana korshinskii plantation in a typical agro-pastoral ecotone in northern China over a 50-a period

Bacterial and fungal communities play critical roles in reestablishing vegetation structure, function and biodiversity in ecosystem restoration in arid and semi-arid areas. However, the long-term successional changes in bacterial and fungal communities that occur with artificial vegetation development are not fully understood. In this study, we investigated the successional changes in bacterial and fungal communities in Caragana korshinskii Kom. plantation over a period of 50 a (6, 12, 18, 40 and 50 a) and their relationships with key soil environmental factors in a typical agro-pastoral ecotone, northern China. The results showed that bacterial and fungal diversities (α- and β-diversity) were significantly affected by plantation age; moreover, the change in fungal community was more evident than that in bacterial community. Soil samples from 12 a plantation had the highest (P<0.05) bacterial and fungal α-diversity (i.e., abundance-based coverage estimator (ACE) and Chao1 index) at 0-10 cm depth compared with other samples. However, soil samples from plantation at the late recovery stage (40-50 a) had the highest α-diversity at 10-20 cm depth. Soil bacterial community was not significantly affected by plantation age at the genus level; but, soil fungal community was significantly affected at the genus level. Overall, Mortierella and Chaetomium were the dominant genera at natural recovery stage (0 a); Inocybe was the dominant genus at the early recovery stage (6-12 a); Inocybe and Mortierella were the dominant genera at the mid-recovery stage (12-40 a); And Mortierella, Cladosporium and Humicola were the dominant genera at the late recovery stage (40-50 a). Redundancy analysis (RDA) showed that β-glucosidase activity, total nitrogen and soil organic carbon were closely associated with bacterial community composition, while alkaline phosphatase, urease activity and total nitrogen were associated with fungal community composition, indicating that changes in enzyme activity and soil nutrients were the most important determinants of dominant genera. Furthermore, pathogenic microorganisms (Cladosporium and Humicola) were dominant in soils from 40-50 a plantation, which may affect plant growth, resulting in the decline of C. korshinskii plantation. Overall, the findings of this study improve the understanding of ecological patterns of bacterial and fungal communities in artificial vegetation and provide an important scientific basis for comprehensive ecological restoration management in arid and semi-arid areas.     

Water utilization of typical plant communities in desert steppe,China

Water is a limiting factor in the restoration and construction of desert steppe. Exploring plant water sources is necessary to understand soil-plant interactions and species coexistence; however, water sources of major plant communities within the desert steppe of Ningxia Hui Autonomous Region, China remain poorly understood. In this study, we analyzed the water uptake of plants in four typical communities: Agropyron mongolicum Keng.; Sophora alopecuroids Linn.; Stipa breviflora Griseb., and Achnatherum splendens (Trin.) Nevski communities. Stable isotopes δD and δ¹⁸O in the xylem of plant and soil water at different soil depths were analyzed. An IsoSource model was used to determine the soil depths from which plants obtained water. Results showed that A. mongolicum community obtained water predominantly from 0-20 and 40-80 cm depth, S. alopecuroids community from 0-20 cm depth, S. breviflora community from 0-40 cm depth, and A. splendens community from 0-20 and 80-140 cm depths. S. alopecuroides had a wider range of soil depths for water extraction, i.e., utilizing different water sources depending on habitat, and the plasticity of its water uptake pattern determined its role in different communities. Water source of plants relayed heavily on the distribution of their roots. Competition for soil water exists between different plant life forms in the sierozem habitat (A. mongolicum, S. alopecuroids, and S. breviflora communities), and in the sandy soil habitat (A. splendens community). The use of soil water by A. splendens community is more spatially differentiated, and shrubs and herbs can coexist stably. Under the pattern of extended drought period in the future, sierozem habitat may be more favorable for the formation of a dominant monoculture community type of perennial fibrous plants. In aeolian sandy soil habitat, A. splendens had a strong competitive advantage, and the growth of shallow-rooted plants was easily suppressed.     

Scenario simulation of water retention services under land use/cover and climate changes: a case study of the Loess Plateau,China

Comprehensive assessments of ecosystem services in environments under the influences of human activities and climate change are critical for sustainable regional ecosystem management. Therefore, integrated interdisciplinary modelling has become a major focus of ecosystem service assessment. In this study, we established a model that integrates land use/cover change (LUCC), climate change, and water retention services to evaluate the spatial and temporal variations of water retention services in the Loess Plateau of China in the historical period (2000-2015) and in the future (2020-2050). An improved Markov-Cellular Automata (Markov-CA) model was used to simulate land use/land cover patterns, and ArcGIS 10.2 software was used to simulate and assess water retention services from 2000 to 2050 under six combined scenarios, including three land use/land cover scenarios (historical scenario (HS), ecological protection scenario (EPS), and urban expansion scenario (UES)) and two climate change scenarios (RCP4.5 and RCP8.5, where RCP is the representative concentration pathway). LUCCs in the historical period (2000-2015) and in the future (2020-2050) are dominated by transformations among agricultural land, urban land and grassland. Urban land under UES increased significantly by 0.63×10³ km²/a, which was higher than the increase of urban land under HS and EPS. In the Loess Plateau, water yield decreased by 17.20×10⁶ mm and water retention increased by 0.09×10⁶ mm in the historical period (2000-2015), especially in the Interior drainage zone and its surrounding areas. In the future (2020-2050), the pixel means of water yield is higher under RCP4.5 scenario (96.63 mm) than under RCP8.5 scenario (95.46 mm), and the pixel means of water retention is higher under RCP4.5 scenario (1.95 mm) than under RCP8.5 scenario (1.38 mm). RCP4.5-EPS shows the highest total water retention capacity on the plateau scale among the six combined scenarios, with the value of 1.27×10⁶ mm. Ecological restoration projects in the Loess Plateau have enhanced soil and water retention. However, more attention needs to be paid not only to the simultaneous increase in water retention services and evapotranspiration but also to the type and layout of restored vegetation. Furthermore, urbanization needs to be controlled to prevent uncontrollable LUCCs and climate change. Our findings provide reference data for the regional water and land resources management and the sustainable development of socio-ecological systems in the Loess Plateau under LUCC and climate change scenarios.     

Response of plant physiological parameters to soil water availability during prolonged drought is affected by soil texture

Soil water deficit is increasingly threatening the sustainable vegetation restoration and ecological construction on the Loess Plateau of China due to the climate warming and human activities. To determine the response thresholds of Amygdalus pedunculata (AP) and Salix psammophila (SP) to soil water availability under different textural soils, we measured the changes in net photosynthetic rate (P_n), stomatal conductance (G_s), intercellular CO₂ concentration (C_i), leaf water potential (ψ_w), water use efficiency (WUE) and daily transpiration rate (T_d) of the two plant species during soil water content (SWC) decreased from 100% field capacity (FC) to 20% FC in the sandy and loamy soils on the Loess Plateau in the growing season from June to August in 2018. Results showed that P_n, G_s, WUE and T_d of AP and SP remained relatively constant at the beginning of soil water deficit but decreased rapidly as plant available soil water content (PASWC) fell below the threshold values in both the sandy and loamy soils. The PASWC thresholds corresponding to P_n, G_s and C_i of AP in the loamy soil (0.61, 0.62 and 0.70, respectively) were lower than those in the sandy soil (0.70, 0.63 and 0.75, respectively), whereas the PASWC thresholds corresponding to P_n, G_s and C_i of SP in the loamy soil (0.63, 0.68 and 0.78, respectively) were higher than those in the sandy soil (0.58, 0.62 and 0.66, respectively). In addition, the PASWC thresholds in relation to T_d and WUE of AP (0.60 and 0.58, respectively) and SP (0.62 and 0.60, respectively) in the loamy soil were higher than the corresponding PASWC thresholds of AP (0.58 and 0.52, respectively) and SP (0.55 and 0.56, respectively) in the sandy soil. Furthermore, the PASWC thresholds for the instantaneous gas exchange parameters (e.g., P_n and G_s) at the transient scale were higher than the thresholds for the parameters (e.g., T_d) at the daily scale. Our study demonstrates that different plant species and/or different physiological parameters exhibit different thresholds of PASWC and that the thresholds are affected by soil texture. The result can provide guidance for the rational allocation and sustainable management of reforestation species under different soil conditions in the loess regions.     

黄河上游植被覆盖度空间分布特征及其影响因素

采用2000—2015年MODIS1M NDVI数据计算黄河上游年最大植被覆盖度 (FVC),了解空间分布状况及变化特征;同时,采用了一种基于统计学原理的地理探测器模型,考虑非气候类环境因素、气候类环境因素和人类活动因素,使用相应的代理变量对黄河上游FVC空间分布的影响因素做定量研究。研究表明：① 黄河上游FVC总体上以改善为主,空间分布特征变化不大;② 单因子方面,降水量(q值0.669)是该地区FVC空间分布的主要影响因素,其他因子的影响作用存在区域差异;③ 降水分别和土壤类型、土地利用方式的交互作用(q值0.777、0.775)对研究区FVC空间分布起主导作用,土壤类型和土地利用的影响作用在一定高程、降水条件下才得以体现;④ 总体上,气候类环境因素>非气候类环境因素>人类活动因素,人类活动在与降水等环境因子的共同作用能够更充分地解释FVC空间分布;⑤ 对研究区的生态恢复应重点放在降水的充分利用和土地利用方式改进等方面。     

Tree-ring δ15N of Qinghai spruce in the central Qilian Mountains of China: Is pre-treatment of wood samples necessary?

A knowledge of the tree-ring stable nitrogen isotope ratio (δ¹⁵N) can deepen our understanding of forest ecosystem dynamics by indicating the long-term availability, cycling and sources of nitrogen (N). However, the radial mobility of N blurs the interannual variations in the long-term N records. Previous studies of the chemical extraction of tree rings before analysis had produced inconsistent results and it is still unclear whether it is necessary to pre-treat wood samples from specific tree species to remove soluble N compounds before determining the δ¹⁵N values. We compared the effects of pre-treatment with organic solvents and hot ultrapure water on the N concentration and δ¹⁵N of tree rings from endemic Qinghai spruce (Picea crassifolia) growing in the interior of the central Qilian Mountains, China, during the last 60 a. We assessed the effects of different preparation protocols on the removal of the labile N compounds and investigated the need to pre-treat wood samples before determining the δ¹⁵N values of tree rings. Increasing trends of the tree-ring N concentration were consistently observed in both the extracted and unextracted wood samples. The total N removed by extraction with organic solvents was about 17.60%, with a significantly higher amount in the sapwood section (P<0.01). The δ¹⁵N values of tree rings decreased consistently from 1960 to 2019 in both the extracted and unextracted wood samples. Extraction with organic solvents increased the δ¹⁵N values markedly by about 5.2‰ and reduced the variations in the δ¹⁵N series. However, extraction with hot ultrapure water had little effect, with only a slight decrease in the δ¹⁵N values of about 0.5‰. Our results showed that the radial pattern in the inter-ring movement of N in Qinghai spruce was not minimized by extraction with either organic solvents or hot ultrapure water. It is unnecessary to conduct hot ultrapure water extraction for the wood samples from Qinghai spruce because of its negligible effect on the removal of the labile N. The δ¹⁵N variation trend of tree rings in the unextracted wood samples was not influenced by the heartwood-sapwood transition zone. We suggest that the δ¹⁵N values of the unextracted wood samples of the climate-sensitive Qinghai spruce could be used to explore the ecophysiological dynamics while focusing on the long-term variations.     

临潼区土地整理中新增耕地土壤养分现状研究

本研究目的在于分析土地整理项目中新增耕地土壤养分含量的特征,明确生产条件改善后影响土地生产能力的重要因素,为更好地发挥土地整理效果提供依据。采集临潼区新丰土地整理项目中道路交通用地、水利设施用地、疏林地和对照样地等4种土地利用类型经整理后的新增耕地17个土壤样品,测定其容重、有机质、全氮、速效磷、速效钾、代换量、pH值和全盐量,运用SPSS统计软件分析土壤养分状况。结果表明:新增耕地土壤养分含量较低,土壤养分中容重、有机质、全氮、速效磷、速效钾、pH值、全盐和代换量含量平均为1.54 g/cm3,5.37 g/kg,0.54 g/kg,5.42 mg/kg,73.23 mg/kg,8.51,573.81 mg/kg和12.41 cmol/kg;原有土地利用类型对养分含量影响较大;土壤中各养分间相关程度较低,容重-有机质、速效钾-代换量和全盐量、代换量-全盐量具有明显相关性,相关系数分别为-0.54,0.58,-0.62和-0.68,其余养分含量之间无明显相关性。可以看出:新增耕地利用时间较短,养分积累不足,土壤有机质含量低是导致肥力较低的主要原因,土壤养分水平成为制约整理后土地生产能力和影响产量差异的的重要因素,应将提高土壤有机质含量作为培肥中心环节。     

沙地樟子松人工林土壤氮矿化特征

土壤氮(N)的有效性是沙地林生态系统生产力和稳定性的关键限制因子。本研究以科尔沁沙地樟子松人工林为研究对象,分析测定了10~60 a林龄土壤NH₄⁺-N、NO₃^--N和矿质N含量、氨化速率、硝化速率和N矿化速率的变化规律。结果表明：樟子松人工林土壤NH₄⁺-N、NO₃^--N和矿质N含量,随着不同林龄在10~60 a内表现出增加的趋势,其中10~30 a增幅较小,40~60 a显著提高;随着土层深度增加而减小,主要集中在0~20 cm,呈现出表聚性。不同林龄樟子松人工林土壤氨化速率、硝化速率和矿化速率均随林龄增加而增加,随土层深度的增加而降低,其中0~40 cm土层明显大于40~100 cm。通过双因素方差分析,得出林龄与土层对各矿化指标影响显著。土壤矿化指标与人工林地上樟子松株高、胸径、冠幅以及土壤有机碳、全氮、碱解氮有显著的正相关关系。通过冗余分析(RDA)表明,影响不同林龄土壤矿化指标的主要环境因子为全氮、全磷、有机碳;影响不同土层土壤矿化指标的主要环境因子为全氮、碱解氮、有机碳。因此,樟子松人工林能够改善沙地土壤N的有效性,幼龄林和中龄林改善效果较小,近成熟林和成熟林改善效果明显;对表层土的改善效果优于深层土;土壤肥力是影响土壤矿化指标的主要环境因子。     

昆虫病原线虫JLSY 003的鉴定及其生物学特性测定

经28S rDNA和ITS序列分析,从吉林省松原市土样中分离到的昆虫病原线虫JLSY 003为Oscheius myriophila品系.本研究以大蜡螟末龄幼虫为寄主,测定该线虫的生物学特性.结果表明,JLSY 003浓度为80IJs/larva时具有较高的侵染力,致死中时间LT50为15.409 h,侵染24、48h...     

Diversity of soil bacteria and fungi communities in artificial forests of the sandy-hilly region of Northwest China

Soil erosion is a serious issue in the sandy-hilly region of Shanxi Province, Northwest China. There has been gradual improvement due to vegetation restoration, but soil microbial community characteristics in different vegetation plantation types have not been widely investigated. To address this, we analyzed soil bacterial and fungal community structures, diversity, and microbial and soil environmental factors in Caragana korshinskii Kom., Populus tomentosa Carr., Populus simonii Carr., Salix matsudana Koidz, and Pinus tabulaeformis Carr. forests. There were no significant differences in the dominant bacterial community compositions among the five forest types. The alpha diversity of the bacteria and fungi communities showed that ACE (abundance-based coverage estimator), Chao1, and Shannon indices in C. korshinskii forest were significantly higher than those in the other four forest types (P<0.05). Soil organic matter, total nitrogen, and urease had a greater impact on bacterial community composition, while total nitrogen, β-glucosidase, and urease had a greater impact on fungal community composition. The relative abundance of beneficial and pathogenic microorganisms was similar across all forest types. Based on microbial community composition, diversity, and soil fertility, we ranked the plantations from most to least suitable as follows: C. korshinskii, S. matsudana, P. tabulaeformis, P. tomentosa, and P. simonii.     

Characteristics of root pullout resistance of Caragana korshinskii Kom. in the loess area of northeastern Qinghai-Tibet Plateau,China

Roots exert pullout resistance under pullout force, allowing plants to resist uprooting. However, the pullout resistance characteristics of taproot-type shrub species of different ages remain unclear. In this study, in order to improve our knowledge of pullout resistance characteristics of taproot systems of shrub species, we selected the shrub species Caragana korshinskii Kom. in different growth periods as the research plant and conducted in situ root pullout test. The relationships among the maximum pullout resistance, peak root displacement, shrub growth period, and aboveground growth indices (plant height and plant crown breadth) were analyzed, as well as the mechanical process of uprooting. Pullout resistance of 4-15 year-old C. korshinskii ranged from 2.49 (±0.25) to 14.71 (±4.96) kN, and the peak displacement ranged from 11.77 (±8.61) to 26.50 (±16.09) cm. The maximum pullout resistance and the peak displacement of roots increased as a power function (R²=0.9038) and a linear function (R²=0.8242) with increasing age, respectively. The maximum pullout resistance and the peak displacement increased with increasing plant height; however, this relationship was not significant. The maximum pullout resistance increased exponentially (R²=0.5522) as the crown breadth increased. There was no significant relationship between the peak displacement and crown breadth. The pullout resistance and displacement curve were divided into three stages: the initial nonlinear growth, linear growth, and nonlinear stages. Two modes of failure of a single root occurred when the roots were subjected to vertical loading forces: the synchronous breakage mode and the periderm preferential breakage mode. These findings provide a foundation for further investigation of the soil reinforcement and slope protection mechanisms of this shrub species in the loess area of northeastern Qinghai-Tibet Plateau, China.     

Effects of native and invasive Prosopis species on topsoil physiochemical properties in an arid riparian forest of Hormozgan Province,Iran

Biological invasions can alter soil properties within the range of their introduced, leading to impacts on ecosystem services, ecosystem functions, and biodiversity. To better understand the impacts of biological invasions on soil, we compared topsoil physiochemical properties at sites with invasive alien tree species(Prosopis juliflora), native tree species(Prosopis cineraria, Acacia tortilis, and Acacia ehrenbergiana), and mixed tree species in Hormozgan Province of Iran in May 2018. In this s...     

Elevated CO2 increases shoot growth but not root growth and C:N:P stoichiometry of Suaeda aralocaspica plants

The purpose of the current study was to investigate the eco-physiological responses, in terms of growth and C:N:P stoichiometry of plants cultured from dimorphic seeds of a single-cell C₄ annual Suaeda aralocaspica (Bunge) Freitag and Schütze under elevated CO₂. A climatic chamber experiment was conducted to examine the effects of ambient (720 μg/L) and CO₂-enriched (1440 μg/L) treatments on these responses in S. aralocaspica at vegetative and reproductive stages in 2012. Result showed that elevated CO₂ significantly increased shoot dry weight, but decreased N:P ratio at both growth stages. Plants grown from dimorphic seeds did not exhibit significant differences in growth and C:N:P stoichiometric characteristics. The transition from vegetation to reproductive stage significantly increased shoot:root ratio, N and P contents, but decreased C:N, C:P and N:P ratios, and did not affect shoot dry weight. Moreover, our results indicate that the changes in N:P and C:N ratios between ambient and elevated CO₂ are mainly caused by the decrease of N content under elevated CO₂. These results provide an insight into nutritional metabolism of single-cell C₄ plants under climate change.     

土地整治的生态系统服务价值评估--参数优化与实证

为定量研究具体区域土地整治前后生态系统服务价值的变化情况,本研究以庆阳市土地整治子项目为例,依据生态系统服务价值评估方法的局限性对其参数进行相应优化的基础上,对研究区内土地整治前后的生态系统服务价值变化进行实证分析。结果表明:①通过尺度推演,将宏观大尺度的生态系统服务价值评估方法进行了市场因素修正、特殊生态系统类型价值当量的制定以及针对区域内部差异的NPP修正,降低了当量因子法应用到具体尺度上的局限性,使其更具合理性。②基于优化参数的实证得到,土地整治前后4 a间生态系统服务价值由2.88×10^8元减少到2.76×10^8元,减少了4.31%。在一级分类体系下,只有调节服务的生态系统服务价值减少了1.84×10^7元,其余服务均相对增加;在二级分类体系下,多项服务较土地整治前均有所增加,且食物生产服务的增加量最大为8.35×10^6元,原料生产、生物多样性以及4项调节服务均相对减少。本研究的实证结果更符合具体区域实际情况,参数优化后的方法可用于坡耕地转变为梯田的生态系统服务价值评估,为区域内部残丘陵、沟谷、坡地等地貌类型的黄土丘陵沟壑区土地整治的生态系统服务价值评估可提供参考。     

The impact of land use change on soil organic carbon and labile organic carbon stocks in the Longzhong region of Loess Plateau

Land use change (LUC) is widely recognized as one of the most important driving forces of global carbon cycles. The soil organic carbon (SOC) and labile organic carbon (LOC) stores were investigated at arable land (AL), artificial grassland (AG), artificial woodland (AW), abandoned arable land (AAL) and desert steppe (DS) in the Longzhong region of the Loess Plateau in Northwest China. The results showed that conversions from DS to AL, AL to AG and AL to AAL led to an increase in SOC content, while the conversion from DS to AW led to a decline. The differences in SOC content were significant between DS and AW at the 20-40 cm depth and between AL and AG at the 0-10 cm depth. The SOC stock in DS at the 0-100 cm depth was 39.4 t/hm 2 , increased by 28.48% after cultivation and decreased by 19.12% after conversion to AW. The SOC stocks increased by 2.11% from AL to AG and 5.10% from AL to AAL. The LOC stocks changed by a larger magnitude than the SOC stocks, which suggests that it is a more sensitive index of carbon dynamics under a short-term LUC. The LOC stocks increased at 0-20 cm and 0-100 cm depths from DS to AW, which is opposite to that observed for SOC. The proportion of LOC to SOC ranged from 0.14 to 0.20 at the 0-20 cm depth for all the five land use types, indicating low SOC dynamics. The allocation proportion of LOC increased for four types of LUC conversion, and the change in magnitude was largest for DS to AW (40.91%). The afforestation, abandonment and forage planting on arable land led to sequestration of SOC; the carbon was lost initially after afforestation. However, the carbon sink effect after abandonment may not be sustainable in the study area.     

基于TaqMan MGB探针的葡萄茎枯病菌实时荧光PCR检测方法

 葡萄茎枯病菌是我国进境植物检疫性有害生物。带菌植物材料是病害传播的重要载体,准确、灵敏、快速的检测方法是严格执行口岸检疫措施及研究病害防控措施的有力工具。根据葡萄茎枯病菌及其近似种的细胞骨架蛋白(Actin)基因序列差异,设计并合成1对引物和1条特异性TaqMan-MGB探针,建立了葡萄茎枯病菌的实时荧光PCR检测方法。通过对反应体系的优化,确定了葡萄茎枯病菌的实时荧光PCR最佳反应条件:引物终浓度为0.6 μmol·L^-1,探针终浓度为0.6 μmol·L^-1。灵敏度试验结果显示,最低检测限为总DNA含量20 pg(20 μL反应体系)。此方法快速灵敏,整个反应1 h即可完成,检测过程完全闭管,无需PCR产物后续处理,为快速检测葡萄茎枯病菌提供了重要参考。该方法用于口岸疑似菌株检测,可成功检测出葡萄茎枯病菌。本研究建立的基于TaqMan MGB探针的荧光定量PCR检测方法为葡萄茎枯病菌的早期快速检测监测提供了有力工具。     

UeRbf1调控菰黑粉菌丝状生长和致病力的作用研究

 基于菰黑粉菌的全基因组序列,克隆得到了UeRbf1(GenBank登录号:MW375682),该基因的开放阅读框为1 281 bp,无内含子,编码426 个氨基酸,经预测具有3个C₂H₂锌指结构域,提示其具有转录调控功能。进一步在菰黑粉菌中敲除UeRbf1后进行体外生长及侵染试验,发现:在体外培养时,UeRbf1的缺失没有改变菌株的生长速率及单倍体形态,也未改变两个性亲和UeRbf1缺失突变体的融合及菌丝形成能力,但是融合菌丝的丝状生长能力显著减弱,而且在侵染时UeRbf1缺失突变体丧失了菌丝形成及丝状生长能力,人工接种野茭后也无法使其茎部膨大。以上结果表明,UeRbf1是调控菰黑粉菌丝状生长和侵染能力的一个重要因子。另外, UeRbf1缺失突变后,在体外融合及侵染时相关丝状生长和致病力的调控因子Biz1、Clp1、Hdp1和UeKpp6呈下调表达,其中Biz1的下调表达最为显著,在突变体中几乎不表达,说明UeRbf1对菰黑粉菌丝状生长和致病力的影响,可能是通过调控Biz1来实现的。     

基于TaqMan MGB探针的葡萄茎枯病菌实时荧光PCR检测方法

 葡萄茎枯病菌是我国进境植物检疫性有害生物。带菌植物材料是病害传播的重要载体,准确、灵敏、快速的检测方法是严格执行口岸检疫措施及研究病害防控措施的有力工具。根据葡萄茎枯病菌及其近似种的细胞骨架蛋白(Actin)基因序列差异,设计并合成1对引物和1条特异性TaqMan-MGB探针,建立了葡萄茎枯病菌的实时荧光PCR检测方法。通过对反应体系的优化,确定了葡萄茎枯病菌的实时荧光PCR最佳反应条件:引物终浓度为0.6 μmol·L^-1,探针终浓度为0.6 μmol·L^-1。灵敏度试验结果显示,最低检测限为总DNA含量20 pg(20 μL反应体系)。此方法快速灵敏,整个反应1 h即可完成,检测过程完全闭管,无需PCR产物后续处理,为快速检测葡萄茎枯病菌提供了重要参考。该方法用于口岸疑似菌株检测,可成功检测出葡萄茎枯病菌。本研究建立的基于TaqMan MGB探针的荧光定量PCR检测方法为葡萄茎枯病菌的早期快速检测监测提供了有力工具。     

Impact of land use/land cover types on surface humidity in northern China in the early 21st century

In the context of global change, it is essential to promote the rational development and utilization of land resources, improve the quality of regional ecological environment, and promote the harmonious development of human and nature for the regional sustainability. We identified land use/land cover types in northern China from 2001 to 2018 with ENVI images and ArcGIS software. Meteorological data were selected from 292 stations in northern China, the potential evapotranspiration was calculated with the Penman-Monteith formula, and reanalysis humidity and observed humidity data were obtained. The reanalysis minus observation (RMO, i.e., the difference between reanalysis humidity and observed humidity) can effectively characterize the impact of different land use/land cover types (forestland, grassland, cultivated land, construction land, water body and unused land) on surface humidity in northern China in the early 21^st century. The results showed that from 2001 to 2018, the area of forestland expanded (increasing by approximately 1.80×10⁴ km²), while that of unused land reduced (decreasing by approximately 5.15×10⁴ km²), and the regional ecological environment was improved. Consequently, land surface in most areas of northern China tended to be wetter. The contributions of land use/land cover types to surface humidity changes were related to the quality of the regional ecological environment. The contributions of the six land use/land cover types to surface humidity were the highest in northeastern region of northern China, with a better ecological environment, and the lowest in northwestern region, with a fragile ecological environment. Surface humidity was closely related to the variation in regional vegetation coverage; when the regional vegetation coverage with positive (negative) contributions expanded (reduced), the land surface became wetter. The positive contributions of forestland and water body to surface humidity were the greatest. Unused land and construction land were associated with the most serious negative contributions to surface humidity. Affected by the regional distribution pattern of vegetation, surface humidity in different seasons decreased from east to west in northern China. The seasonal variation in surface humidity was closely related to the growth of vegetation: surface humidity was the highest in summer, followed by autumn and spring, and the lowest in winter. According to the results, surface humidity is expected to increase in northeastern region of northern China, decrease in northern region, and likely increase in northwestern region.