Spectroscopic Determination of Soil Organic Carbon and Total Nitrogen Content in Pasture Soils

To explore a convenient and efficient approach for determining the content of soil organic carbon (SOC) and total nitrogen (TN), this study focused on capturing the features value of SOC and TN by applying the method of wavelet analysis and wavelet transformation. The soil used in the study was sampled from the pastures in Fukang City of Xinjiang Uygur Autonomous Region, China. The soil samples were tested by using a combined approach of chemical analysis and spectroscopy measurements. It was found that reflectance at 400–2500 nm was more strongly correlated to SOC than to TN. The maximum negative r values between reflectance and SOC + TN at 2309 nm was –0.81 (P < 0.01), and SOC/TN at 1693 was –0.48 (P < 0.05). The maximum correlation coefficient between SOC, TN, and wavelet coefficient was more than 0.96 compared to the relationship among SOC, TN, and spectral reflectance. By using continuous wavelet transformation (CWT), it was found that the maximum correlation coefficients were 0.981 at 2328 nm of scale 13 for SOC and 0.968 at 1741 nm of scale 6 for TN. These results also suggested that wavelet analysis was a better method for capturing the absorption features of soil properties and determining SOC and TN content.     

长期施肥对稻田土壤活性有机碳和氮的影响

土壤活性有机碳、氮是土壤有机碳、氮中最活跃的组分,在土壤碳、氮循环过程中具有重要作用。以湖南3个长期定位试验点（桃源、宁乡、桃江）为研究对象,分析测定表层土壤MBC、MBN、DOC、DON,研究长期施肥对土壤活性有机碳、氮（MBC＋DOC,MBN＋DON）的影响。结果表明,长期施用化肥及配施有机肥均能提高土壤活性有机碳、氮的含量。与对应的无肥处理（CK）相比,长期单施化肥（NPK）使土壤活性有机碳、氮的提高幅度分别为3.3%~21.0%和3.3%~27.1%,长期有机肥施用提高幅度分别为48.7%~84.8%和17.9%~105.8%,且土壤活性有机碳、氮含量随有机肥施用量的提高而增加,3个试验点活性有机碳大小顺序为桃源〉宁乡〉桃江。土壤活性有机碳与土壤有机碳（SOC）的累积速率呈显著正相关关系（P〈0.05）,而与SOC的含量关系不大。桃源土壤粘粒含量较高可能是土壤SOC累积速率快的主要原因;宁乡和桃江的粘粒含量和有机碳投入量相差不大,桃江较高的初始SOC水平影响了SOC积累速率;桃源和宁乡试验点的土壤活性氮含量相差不大,约是桃江的1.8倍。与土壤全氮（TN）相比,全氮的积累速率与土壤活性氮的关系更为密切,两者间有极显著的相关关系（P〈0.01）。     

Standardization of Automated Vario EL III CHNS Analyzer for Total Carbon and Nitrogen Determination in Soils

The traditional methods for determination of carbon (C) and nitrogen (N) are cumbersome and time-consuming. With the development of automated methods, the time taken to analyze a sample has been reduced considerably. However, these need to be standardized with respect to the type and weight of soil sample used. A study on the determination of soil C and N was carried out to evaluate sample particle size and weight effects on C and N determined by a CHNS analyzer and conventional methods. Three texturally different soils were analyzed for C and N with the CHNS analyzer and traditional methods. The C and N contents measured with the CHNS analyzer were greater than those measured with traditional methods. With finer (<0.25-mm) fractioned samples, C and N contents were greater than in the coarse-fractioned. Carbon and N contents were greater with a sample weight of 10 than with 25 mg.     

四川盆地水稻土有机碳与全氮的时空变异及影响因素研究

利用全国第二次土壤普查数据与2008年采样数据对四川盆地水稻土有机碳和全氮的含量水平变化进行了研究。结果表明:研究区1982年和2008年3种水稻土亚类有机碳含量均呈现潜育型水稻土显著高于淹育型和潴育型水稻土。研究区1982年3种水稻土亚类全氮含量呈现潜育型水稻土显著高于淹育型水稻土,2008年则为潜育型水稻土显著高于淹育型和潴育型水稻土。26年间水稻土有机碳与全氮含量均增长明显,且淹育型与潜育型两种水稻土的增长幅度高于潴育型水稻土。1982年至2008年,平坝、冲沟和坡脚稻田中有机碳及全氮含量增长明显,且大致呈从平坝、冲沟到坡中上部递减;冲积物和泥岩发育的水稻土有机碳及全氮因易于积累而含量更高;质地黏重的土壤有机碳与全氮的含量较高,同时也比质地偏轻的土壤更利于碳、氮的积累;冬水田与稻–油轮作的农田土壤有机碳与全氮的含量与增长幅度显著高于稻–麦轮作田。     

武夷山不同海拔典型森林土壤有机碳和全氮储量分布特征

为探究武夷山森林土壤碳氮储量的分布特征,以武夷山国家公园不同海拔高度(600,1 000,1 400 m)的典型森林土壤为研究对象,研究土壤有机碳和全氮含量及储量随海拔高度的变化规律,分析了影响土壤有机碳和全氮储量变化的因子。结果表明:随着海拔的升高,土壤有机碳和全氮的含量在0—5 cm土层和5—10 cm土层变化规律不同,5—10 cm土层的土壤碳氮含量随海拔变化趋势更为明显,而0—5 cm土层的土壤碳氮含量表现为1 000 m海拔较高; 海拔1 000 m的土壤C/N明显高于海拔1 400 m和600 m; 在土壤有机碳和全氮储量方面,1 400 m明显高于1 000 m和600 m,且高海拔区域土壤碳氮储量的变化幅度显著大于低海拔区域,两土层间差异不显著; 相关分析和RDA分析表明细根C/N和土壤温度是影响土壤有机碳和全氮储量的主导因子。综上所述,土壤有机碳和全氮的分布随海拔升高并非线性增长,受到气候、植被特征及土壤状况的综合影响,高海拔地区土壤碳氮储量对气候变化的响应更为敏感。     

城市不同功能区绿地土壤微生物量碳氮及溶解性碳氮分布特征及影响因素

为研究城市功能分区对绿地土壤活性碳氮的影响,选择合肥市不同功能区(老商业区、老工业区、居民服务区、近郊森林公园)绿地土壤为研究对象,分析了土壤微生物量碳氮(MBC、MBN)、溶解性碳氮(DOC、DON)、有机碳(SOC)、全氮(TN)以及土壤基本理化性质的差异及其相互关系。结果表明,功能区类型对土壤微生物量碳氮及溶解性碳氮影响显著(P0.05),且0~30 cm土层含量均为近郊森林公园高于城区内各功能区。土层厚度对MBC、DOC、DON含量影响显著,MBC、DON在各功能区中均随土层深度增加而递减,森林公园MBN、DOC均为10~20 cm含量最高。不同功能区MBC/MBN在6~14间波动,以此预测本研究范围内土壤微生物可能以真菌和放线菌为主,细菌较少。本研究未证实DOC与MBC、MBC与DON、DON与MBN间的相关性,MBC+DOC、MBN+DON比MBC、DOC、DON、MBN更能反应土壤活性碳氮库与土壤微生物量。总体来说,相比城区内各功能区,近郊森林公园更利于土壤生物量碳氮及溶解性有机碳氮的积累。     

NIR Spectroscopy: An Alternative for Soil Analysis

Advances in laboratory instrumentation and chemometrics provide alternatives to traditional methods of conducting soil chemical analysis. One of these is infrared diffuse reflectance spectroscopy in the near-infrared spectral range (NIRS). Herein we report the results of a multinational study to develop useful calibrations associating NIRS spectra with laboratory-measured results for total soil carbon (C), total soil nitrogen (N), δ¹³C, and δ¹⁵N from a single soil site in Mexico subjected to zero- and conventional-tillage regimens with and without crop residues and crop rotations of maize and wheat across 16 years. Modified partial least squares regression (MPLS) was used to obtain useful NIR predictions for total soil C and N, with ratio performance deviation (RPD) values of 6.8 and 2.6, respectively. Corresponding multiple correlation coefficients (RSQs) for C and N were 0.98 and 0.85, with standard errors of prediction (SEPs) of ±0.45 g C kg^–1 and ±0.09g Nkg^–1, respectively. The generation of δ¹⁵N and δ¹³C models produced different NIR recordings in soils with and without crop residues. Application of discriminant partial least squares (DPLS) statistics to the NIR spectral data allowed us to discriminate soils with and without residues. The prediction confidence for stable isotopes was 90% (internal validation) and 94% (external validation). Modified partial least squares regression was used to estimate δ¹⁵N and δ¹³C. Ratio performance deviation, RSQ, and SEP values obtained for δ¹³C and δ¹⁵N were 2.44 and 3.57, 0.83 and 0.81, ±0.5‰ (parts per thousand) and ±0.45‰ in soils with residues and 2.5 and 3.8, 0.93 and 0.92, and ±0.2‰ and ±0.23‰ in soils without residues, respectively. Overall, results obtained with NIRS were comparable to those obtained using conventional analytical methods, a finding that has wide relevance to agricultural soils and environmental studies in tropical locations. However, further testing is necessary to confirm that the calibration models are neither site nor instrument specific.     

碳氮添加对雨养农田土壤全氮、有机碳及其组分的影响

为探明碳氮添加4年后,土壤全氮、有机碳及其组分(可溶性有机碳、微生物量碳、轻组和重组有机碳)的变化特征,依托布设于甘肃省定西市安定区李家堡镇的不同碳源配施氮素田间定位试验,涉及秸秆、生物质炭、氮素3个因素,秸秆设置为不施、施用秸秆2水平;生物质炭为不施和施用生物质炭2个水平;氮素设置为不施氮、施纯氮50 kg/hm^2、施纯氮100 kg/hm^2 3个水平,共9个处理。结果表明:不同处理下土壤全氮、有机碳及其组分的含量均随土层的加深而降低。添加生物质炭对土壤全氮、有机碳及其组分均具有不同程度的提升效应。添加秸秆对土壤全氮、有机碳和可溶性有机碳、微生物量碳、轻组有机碳均具有显著提升效应,仅在0-5 cm土层对重组有机碳有显著提高。添加氮素可显著提升土壤全氮、有机碳和可溶性有机碳、微生物量碳、轻组有机碳含量。较其他处理,添加生物质炭对土壤全氮、有机碳和重组有机碳的提升效应最高,添加秸秆对可溶性有机碳、微生物量碳、轻组有机碳的提升效果最优。从提升土壤质量的角度出发,推荐秸秆配施氮素模式,该模式下土壤碳素有效性高、易于被微生物利用,有利于作物生长。从提高土壤固碳角度考虑,推荐生物质炭配施氮素模式,该模式有利于碳的封存。     

改良剂对旱地红壤微生物量碳、氮及可溶性有机碳、氮的影响

基于室内模拟培养试验,研究改良剂(生物质炭、过氧化钙)对旱地红壤微生物量碳、氮及可溶性有机碳、氮的影响。试验设置4个处理,即CK、Ca(过氧化钙,1.72g/kg)、C(生物质炭,21.46g/kg)、C+Ca。结果表明:各处理土壤微生物量碳、氮以及可溶性有机碳具有相同的变化趋势,即前期(3d内)都增加较快,在第3天达到最大值,随试验进行有所下降,配施效果优于单施。各处理可溶性有机氮在21d内缓慢增加;第21天时,C+Ca、Ca、C相比CK分别显著增加了62.1%,55.5%,40.9%;35d以后,配施(C+Ca)与单施过氧化钙(Ca)的效果显著优于单施生物质炭(C)和对照(CK)。120d培养期内,配施(C+Ca)处理能够明显提高微生物量碳、氮以及可溶性有机碳、氮的平均含量;微生物量碳的平均含量大小顺序为C+CaCCKCa,微生物量氮的平均含量C+Ca处理显著高于其他处理;可溶性有机碳的平均含量大小顺序为C+CaCaCCK,可溶性有机氮的平均含量C+Ca、Ca处理显著高于CK、C处理。微生物量碳、氮以及可溶性有机碳之间互为极显著正相关(P0.01),而微生物量碳与可溶性有机氮之间呈极显著负相关。因此,生物质炭和过氧化钙能有效提高旱地红壤微生物量碳、氮及可溶性有机碳、氮,且生物质炭与过氧化钙配合施用更有助于土壤改良。     

Comparison of Methods for Determination of Carbon in Calcareous Soils

The global cycle of carbon (C) has raised attention in recent decades due to the great increase in carbon dioxide levels (CO₂) levels in the atmosphere and its influence on climate change. Calcareous soils represent a significant fraction of the areas with potential for agriculture and have differential attributes, such as high calcium contents, magnesium, carbonates, and pH values. These attributes have been ignored in analytical procedures despite these characteristics, resulting in an overestimation or underestimation of the soil carbon. Several methods have been proposed for determining the soil carbon contents, however, studies evaluating the analytical procedures of C quantification methods, considering the soil characteristics, such as the calcareous soils, are needed, in order to improve their accuracy. Therefore, the objective of this work was to evaluate and compare methods for C determination and to propose adjustments in the methodology for calcareous soil analysis. The Yeomans and Bremner (YB) was the most efficient method for quantification of organic C among the wet oxidation methods. On the other hand, the Donagemma (WB) method underestimated the organic carbon contents. The results showed that the samples must be macerated and pretreated with a hydrochloric acid solution for the use of CHNS-O, in order to eliminate carbonates in the form of nodules and concretions.     

Rapid Determination of Carbon,Nitrogen, Silicon,Phosphorus, and Potassium in Sugar Mill By-products,Mill Mud,and Ash using Near Infrared Spectroscopy

This article describes a proof-of-concept exercise to examine the ability of near infrared spectroscopy (NIRS)–based methods to predict the major nutrient properties of sugar mill by-products, particularly mill mud, ash, and mixtures of mud and ash. Sixty mill mud, mixed mud/ash, and ash samples were subsampled three times and analyzed using traditional analytical techniques for carbon (C), nitrogen (N), silicon (Si), phosphorus (P), and potassium (K), and the NIR spectra were recorded. Two different partial least squares (PLS) regression models were constructed, one using all samples and the other without the ash samples included in the model development. Three mud, one mixed mud/ash, and two ash samples were retained for predictive purposes and were not included in the model development process. R² values in the range of 0.77 to 0.98 were obtained for all constituents across both sets of PLS models. The standard errors of prediction (SEP) were similar for both models for N (0.10 and 0.08), P (0.17 and 0.16), and K (0.05 and 0.05). However, the SEP obtained for Si (3.53 and 1.04) and C (1.92 and 1.00) varied between the two models. These preliminary results are very encouraging. Future research will extend to robust NIRS calibrations for these nutrients and develop applications for their use within laboratory or field situations to permit nutrient monitoring in various sugar mill by-products.     

Comparison of Near Infrared Reflectance Spectroscopy with Combustion and Chemical Methods for Soil Carbon Measurements in Agricultural Soils

As interest in soil organic carbon (SOC) dynamics increases, so do needs for rapid, accurate, and inexpensive methods for quantifying SOC. Objectives were to i) evaluate near infrared reflectance (NIR) spectroscopy potential to determine SOC and soil organic matter (SOM) in soils from across Tennessee, USA; and ii) evaluate potential upper limits of SOC from forest, pasture, no-tillage, and conventional tilled sites. Samples were analyzed via dry-combustion (SOC), Walkley–Black chemical SOM, and NIR. In addition, the sample particle size was classified to give five surface roughness levels to determine effects of particle size on NIR. Partial least squares regression was used to develop a model for predicting SOC as measured by NIR by comparing against SOM and SOC. Both NIR and SOM correlated well (R² > 0.9) with SOC (combustion). NIR is therefore considered a sufficiently accurate method for quantifying SOC in soils of Tennessee, with pasture and forested systems having the greatest accumulations.Abbreviations SOC, soil organic carbon; NIR, Near Infrared Reflectance Spectroscopy; MTREC, Middle Tennessee Research and Education Center; RECM, Research and Education Center at Milan; PREC, Plateau Research and Education Center; PLS, Partial least squares.     

尕海湿地区沼泽草甸生长季土壤碳氮组分对增温施氮的响应

[目的] 为探究高寒湿地土壤碳氮组分对气候变暖和氮沉降的响应特征。[方法] 以尕海湿地沼泽草甸为研究对象,采用开顶箱增温(OTC)和外源氮素(NH₄NO₃)添加模拟未来气候变暖及氮沉降试验,分别设置对照(CK)、增温(W)、施氮(N)和增温施氮(WN)4种处理。在试验进行1.5年后对土壤碳氮组分含量进行测定。[结果] (1)开顶箱增温装置提高0—20 cm土层平均温度1.126℃,显著降低0—10 cm土层土壤含水量(SMC)、pH、全氮(TN)、微生物量氮(MBN)、铵态氮(NH₄⁺—N)、有机碳(SOC)和可溶性有机碳(DOC)含量,提高硝态氮(NO₃^-—N)含量。(2)施氮显著降低NH₄⁺—N、SOC和10—20 cm土层微生物生物量碳(MBC)及DOC含量,增加土壤TN、MBN和NO₃^-—N含量。(3)增温施氮显著增加土壤SMC、TN、NO₃^-—N和MBC含量,降低MBN、NH₄⁺—N和DOC含量。(4)相关分析显示,土壤水分与各理化因子均存在正相关性,土壤碳氮组分间均呈正相关性。[结论] 模拟增温施氮缓解尕海湿地植物生长的温度和氮的限制,促进TN的积累,对土壤微生物量碳氮影响较大,导致土壤微生物量碳氮及分布特征发生转换。     

模拟氮沉降对湿性常绿阔叶次生林土壤碳氮组分和酶活性的影响

为了研究氮沉降对次生林土壤碳氮组分和酶活性的影响,以华西雨屏区湿性常绿阔叶次生林为对象,从2014年1月起进行野外定位模拟氮沉降试验,分别设置对照(CK,+0 g/(m^2·a))、低氮(LN,+5 g/(m^2·a))和高氮(HN,+15 g/(m^2·a))3个氮添加水平。在氮沉降进行27个月后,按照腐殖质层和淋溶层表层进行取样,测定不同土层土壤总有机碳(TOC)、可浸提溶解性有机碳(EDOC)、易氧化碳(ROC)、全氮(TN)、硝态氮(NO_3^-—N)和铵态氮(NH_4^+—N)含量以及蔗糖酶、脲酶、酸性磷酸酶和多酚氧化酶活性。结果表明:模拟氮沉降显著增加该次生林腐殖质层土壤的TOC和NH_4^+—N含量,显著增加腐殖质层和淋溶层表层土壤的NO_3^-—N含量,腐殖质层土壤C/N显著升高。淋溶层表层土壤TOC、NH_4^+—N、C/N以及2层土壤的EDOC、ROC、TN和NH_4^+—N/NO_3^-—N均无显著影响。2层土壤的多酚氧化酶活性均随着氮添加量的升高而降低,其中淋溶层表层达到显著差异。模拟氮沉降对蔗糖酶、脲酶和酸性磷酸酶活性均无显著影响。腐殖质层中,NH_4^+—N和NO_3^-—N含量与TOC含量存在极显著正相关关系。2层土壤的多酚氧化酶活性均与NO_3^-—N含量呈极显著负相关。结果说明,模拟氮沉降使该次生林中原本较高的腐殖质层土壤TOC含量进一步显著增加,并且促进土壤无机氮的积累,而模拟氮沉降对多酚氧化酶的抑制作用更加有利于土壤有机质的积累。     

不同松栎混交林土壤溶解性有机碳氮的差异分析

为揭示北亚热带地区不同林分类型的土壤活性碳氮分布特征,在驻马店市南部山区选取4处具有代表性的松栎人工混交林为研究对象,对其0-20 cm土壤的溶解性有机碳（DOC）,溶解性有机氮（DON）等组分进行研究。结果表明:4种林型土壤DOC的含量为麻栎纯林 > 湿地松麻栎混交林 > 火炬松麻栎混交林 > 马尾松麻栎混交林,DON的含量为马尾松麻栎混交林 > 麻栎纯林 > 湿地松麻栎混交林 > 火炬松麻栎混交林,不同林分类型间整体上差异性显著（p<0.05）;在垂直剖面上,4种林型土壤DOC和DON均随土层深度的增加而显著性下降（p<0.05）。4种林分类型DOC/SOC,DON/TN,DOC/DON分别以麻栎纯林、马尾松麻栎混交林、麻栎纯林最高,在垂直空间分布上,差异性整体上不显著。土壤DOC和DON与土壤含水率、土壤硬度、速效钾、全氮均呈显著（p<0.05）或极显著（p<0.01）相关性。     

常规与有机农田土壤团聚体组成及碳氮储量研究

长期施用化肥或连作农田管理模式导致土壤质量退化及碳氮损失加剧。以常规农作大豆和转换后的有机农作大豆田土壤为研究对象,利用土壤物理分组技术,分析了土壤团聚体组成及碳氮储量变化。结果表明,常规农作大豆田转换为有机农作大豆田后,<0.053mm粉粒加黏粒比重显著降低,0.053~0.25mm较小团聚体显著增加,土壤稳定性增大,土壤及团聚体中有机碳和全氮含量都显著增加。有机农作大豆田土壤包被于较小的大团聚体(0.25~2mm)中的<0.053mm细颗粒有机质百分比显著降低,0.053~2mm粗颗粒有机质显著增加。有机农作大豆田土壤及团聚体中碳氮储量都显著高于常规农作大豆田,土壤碳汇和氮汇效应增大。有机农作大豆田土壤稳定性增加,团聚体中碳氮含量显著增加,土壤碳汇效应增强,有机农作方式可能比常规农作方式更有利于土壤碳氮资源持续利用。     

有机物料输入对关中土碳氮影响的后效作用

土壤有机碳氮是土壤肥力的关键因素,有机物料施用是提高土壤有机碳氮的有效措施。研究和比较了不同有机物料输入对土耕层（0—20 cm）土壤有机碳、全氮、可溶性有碳氮及0—200 cm剖面土壤硝态氮和含水量分布变化的后效作用。结果表明,停止施入有机物料两年后,与对照（CK）相比,秸秆与氮磷肥配施（SNP）和生物炭与氮磷肥配施（BNP）的表层（0—20 cm）土壤有机碳（SOC）分别提高了29.5%和29.8%（p<0.05）;氮磷肥（NP）、有机肥与氮磷肥配施（MNP）、秸秆与氮磷肥配施（SNP）和生物炭与氮磷肥配施（BNP）的表层土壤全氮含量较CK分别提高了22.0%,14.3%,24.2%和26.4%（p<0.05）。BNP处理的土壤可溶性有机碳（DOC）显著高于其他处理（p<0.05）,分别比CK,NP,MNP和SNP提高了23.4%,10.9%,21.3%,20.5%;所有施肥处理的土壤可溶性有机氮（DON）均显著高于CK（p<0.05）,分别提高了39.3%,29.3%,34.5%和52.3%。与CK相比,各施肥处理显著提高了表层土壤硝态氮含量（p<0.05）,增加了0—100 cm土层的硝态氮累积量。与NP处理相比,MNP和SNP显著提高了0—200 cm土层的硝态氮累积量（p<0.05）,而BNP则差异不显著。相比CK,施肥处理（NP,MNP,SNP,BNP）可显著提高0—20 cm土层的含水量,增加0—40 cm土层的储水量,且BNP处理显著高于SNP和MNP。总体而言,生物炭在提高和维持表层土壤肥力以及降低剖面硝态氮淋溶风险等方面的后效作用显著优于秸秆和有机肥,是陕西关中地区旱地土上一种较好的有机物料施用方式。     

氮输入对森林土壤有机碳截存与损耗过程的影响

大气氮沉降对受氮限制的陆地生态系统碳截存/损耗的机理尚不清楚,尤其是对土壤有机碳(SOC)的输入、转化和输出过程的认识明显不足。本文论述了外源性氮素(氮沉降、人为增氮)对凋落物分解、土壤有机碳各组分周转的影响,以及土壤呼吸各组分(根系自养呼吸、根际微生物呼吸和SOM分解)对增氮的响应等领域的最新研究进展,指出了在上述研究领域中存在的问题,并提出拟解决的途径以及未来的可能研究方向,以期为该领域的研究提供参考。     

长期氮沉降和地上凋落物处理对半干旱区沙质草地表层土壤碳氮组分的影响

为揭示半干旱区沙质草地生态系统中表层土壤C、N组分对长期氮添加和地上凋落物处理的响应特征,以科尔沁沙地西南部国家野外科学观测研究站建立的长期(9年)氮添加和凋落物处理样地为平台,测定并分析该样地表层土壤环境因子、铵态氮、硝态氮、总有机碳、不同碳氮组分。结果表明:(1)持续9年的氮添加和地上凋落物处理对表层土壤环境因子和不同碳氮组分无交互作用;(2)氮添加处理显著降低土壤pH(p<0.01),增加土壤中硝态氮的含量(p<0.05),其增长幅度为37.57%,并显著增加溶解性有机氮(DON)和易变活性氮(LON)的含量(p<0.01,p<0.05);(3)地上凋落物去除显著降低土壤总有机碳(TOC)、易变缓性碳(IOC)、微生物生物量碳(MBC)和微生物生物量氮(MBN)含量(p<0.05);(4)经过9年氮添加和地上凋落物处理,半干旱区沙质草地表层土壤中不同碳氮组分与土壤环境因子间相关性并不密切。即长期氮添加和地上凋落物处理会改变表层土壤不同碳、氮组分的含量,但并未显著改变各碳、氮组分的比值。研究结果为揭示长期氮添加和地上凋落物处理对半干旱区沙质草地土壤C、N贮存和预测未来土壤生物地球化学元素动态研究提供参考资料。     

微地形对高寒草地土壤有机碳及氮含量的影响

[目的]系统分析高寒草地不同微地形条件下土壤有机碳及氮含量的变化规律,为高寒地区合理利用草地提供理论参考。[方法]在天祝高寒草地区选取7类不同微地形作为研究对象,测定其表层(0—30cm)土壤有机碳及氮含量。[结果]随着土层深度的增加,位于坡底平地,土壤全氮和铵态氮含量呈减小的趋势,位于阴坡不同坡位,土壤全氮含量呈"V"型变化,土壤铵态氮呈减小的趋势,位于阳坡不同坡位,土壤全氮含量呈减小的趋势,土壤铵态氮含量呈先增大后减小的趋势;所有微地形中,随着土层深度的增加,土壤有机碳含量呈减小的趋势;随着土层深度的增加,坡底平地,中坡阳面和中坡阴面微地形中,土壤水解氮含量呈减小的趋势,下坡阳面和下坡阴面微地形中,土壤水解氮含量呈先减小后增大的趋势,上坡阳面和上坡阴面微地形中,土壤水解氮含量呈先增大后减小的趋势。土壤表层全氮、水解氮、有机碳的含量的变化为:下坡位上坡位中坡位,而铵态氮的含量呈现先减小后增大的趋势。[结论]地形的微变化明显影响土壤氮有机碳和素的含量。