AMMONIA AS A SOURCE OF NITROGEN FOR GRASS SWARDS

Aqueous ammonia was injected to a depth of 10 cm into small grass plots in late winter at rates equivalent to 200 and 400 kg N per ha. The effect on yield of DM, seasonal distribution of yield and uptake of N in the herbage was compared with that of single or split applications of ammonium nitrate. Two levels of supplementary irrigation were given in summer. The method of injection minimised damage to the sward and loss of ammonia by volatilization.
Aqueous ammonia and ammonium nitrate (single application) gave similar yields of dry matter, uptake of N and distribution of yield over the season. Ammonia was no more effective than ammonium nitrate under dry conditions. A single application in February of 200 kg N/ha as ammonium nitrate gave a greater total annual yield than the same quantity of N as ammonia or as a split dressing of the solid fertilizer. At 400 kg N/ha, split application gave the highest yields. The uptakes of N in the harvested grass was similar, in each irrigation treatment and at each level of N, for the three methods of applying N, The apparent recovery was low.
Aqueous ammonia, efficiently injected, was a satisfactory form of N for grass at the first cut, but it did not provide a continuing supply of N for crops harvested later in that season.     

Effects of nitrification inhibitor and acid addition to cattle slurry on nitrogen losses and herbage yields

Cattle slurry was applied to grassland on two contrasting soils in autumn and spring between 1987 and 1990. Slurry was applied with or without the addition of acid, to lower the pH to 5·5, and, in autumn only, with or without a nitrification inhibitor. Ammonia volatilization, denitrification and apparent recovery of N by the cut herbage accounted for 61–86% of the ammonium nitrogen (NH₄⁺-N) applied in slurry. Estimates from lysimeter experiments indicated that nitrate leaching from autumn application may have accounted for an additional 1–2% only. Acidifying slurry reduced volatilization losses to 1–12% of the NH₄⁺-N applied, and the nitrification inhibitor halved denitrification losses from autumn applications. Reductions in nitrogen losses were reflected in significant increases in first-cut herbage yields which, for slurry applied in the autumn with acid and the nitrification inhibitor, were generally greater than those from 120 kg ha⁻¹ N as ammonium nitrate applied in the spring. There were no significant differences between treatments at subsequent cuts in each season but, owing to the large increases at first cut, total yields were significantly higher for autumn-applied slurry with acid and nitrification inhibitor.     

THE YIELD RESPONSE OF A TALL FESCUE/WHITE CLOVER SWARD TO NITROGEN RATE AND HARVESTING FREQUENCY

A series of eight fertilizer N rates, ranging from 0 to 377 kg N/ha per year, at increments of 53.8 kg N, was applied to a S170 tall fescue/SlOO white clover sward. The annual rates were split into 8, 6 or 4 equal dressings for harvests at intervals of 3, 4 and 6 weeks, respectively, over a 24-week growth period from March to September, in each of two years. Residual harvests were taken 6 weeks later in October. Curves relating annual herbage yields to N rate were fitted to tbe data. Total herbage DM responses to 108 kg N/ha were small because of the typical white clover yield N rate interaction, but were almost linear from 108 to 323 kg N/ha at all harvesting frequencies. Tbe decline in rate of response at tbe bigher N application levels was less marked with frequent than with infrequent defoliation. Tall fescue DM responses were substantially linear from 0 to 323 kg N/ha. For the first and second years, relative DM yields of total herbage for harvesting frequencies of 3, 4 and 6 weeks were 100:108:120 and 100:111:131, respectively. Total herbage CP response curves were similar to those for DM, but continued to rise more steeply to the highest N rate tested, 377 kg N/ha. Tall fescue CP responses were linear from 0 to 377 kg N/ha. Harvesting frequency did not markedly influence CP yields. Total herbage DM yield was 14% less in the second year compared witb the first. The decline was due to a reduction in white clover, attributed mainly to the effect of repeated N applications, also the subsidiary effects of companion grass and weather.     

The effect of strategic use of fertilizer nitrogen in spring and/or autumn on the productivity of a perennial ryegrass/white clover sward

The productivity of a mixed sward, comprising perennial ryegrass cvs Barlano and Bastion and white clover cvs Donna and Aran, was measured under sixteen fertilizer N treatments. These involved 0.25, 50 and 75 kg N ha^-1 in spring only, in autumn only and in all combinations of spring N and autumn N. A simulated grazing regime of six cuts annually at 3- to 6-week intervals was imposed. Increasing rates of total N application increased total herbage DM regardless of application pattern. Yield response was greater with N applied in the spring, and total herbage DM was higher with high spring N-low autumn N than the reverse. Mean yield responses at the first harvest to 25, 50 and 75 kg ha^-1 N in spring were 13.6, 10.8 and 11.6 kg DM per kg N. Corresponding responses at the final harvest to N rates in the autumn were 7.2, 5.8 and 6.8 kg DM per kg N. Responses were similar at these times for treatments receiving combined spring and autumn N. Over all treatments, mean annual production of total herbage was between 7.08 t ha^-1 DM with no N and 8.19 t ha^-1 with 75 kg ha^-1 N in both spring and autumn. Owing to drought, mean production in year 2 fell by 32% compared with year 1. White clover production fell progressively with increasing N application. Treatments with spring-applied N gave the most marked decrease. White clover was more markedly depressed than the associated grass by the drought in the second year. The mean reductions in white clover content were 0.17, 0.07 and 0.12 percentage units per kg applied N for spring N, autumn N and combinations. Autumn N use depressed white clover less than spring N but the yield response of grass was less. It is concluded that any applied N adversely affects white clover performance to some degree. Where management factors are unfavourable to white clover even strategic N use may not be wise. Instead, it is suggested that a ‘dual-sward’ approach be adopted in practice, namely, grass/white clover swards with no N. and complementary grass swards receiving optimum applied N to give better production at times when grass/white clover swards are relatively less productive.     

Herbage production and nitrogen recovery from slurry injection and fertilizer nitrogen application

An experiment was carried out during 1986 and 1987 to examine the effects of mid-season slurry injection and fertilizer-nitrogen (N) application on herbage dry matter (DM) yield and N recovery. Cattle and pig slurry were injected at 56 and 112 m³ ha⁻¹ into an established sward. Five rates of fertilizer-N, as calcium ammonium nitrate, ranging from 0 to 120 kg ha⁻¹ in 30-kg increments, were superimposed on these treatments, and in both years DM yield was measured in one cut after 70 d regrowth. Slurry treatments increased herbage DM yields significantly ( P < 0-001). The efficiency of slurry total N compared with calcium ammonium nitrate-N averaged 53% in 1986 and 86% in 1987. The mean apparent recovery of slurry total N in herbage was 55% in 1986 and 40% in 1987. Fertilizer-N application increased ( P < 0.001) the mean yields of herbage in both years but when combined with some of the slurry treatments, DM yields over the five N-levels did not differ significantly, giving rise to interactions in 1986 ( P < 0-001) and 1987 ( P < 001). It is concluded that mid-season injection of slurry can be an effective means of utilizing slurry-N in terms of herbage DM production and consequent N use.     

Successive annual applications of organic manures for cut grass: short-term observations on utilization of manure nitrogen

Abstract The recovery of nitrogen (N) from, and the fertilizer‐N value of, low dry‐matter (DM) cattle slurry and farm yard manure (FYM), applied annually to perennial ryegrass swards grown at two sites, on sandy loam and shallow calcareous silty clay loam soils, were studied over a 4‐year period. Slurry or FYM, applied at target rates of either 150 kg N ha^?1 or 300 kg N ha^?1 in either October, February or May/June, in combination with 150 kg N ha^?1 inorganic fertilizer‐N (applied as split dressings before the first and second grass cut), were compared with a set of inorganic fertilizer‐N response treatments. DM yield, N offtake, apparent manure‐N recovery (in herbage) and manure‐N efficiency (compared with inorganic fertilizer‐N) were determined at two silage cuts each summer. Soil mineral nitrogen (SMN) measurements in autumn and spring were used to assess potential N leaching loss over winter and to quantify available N residues in the soil in spring. Apparent manure‐N recovery and manure‐N efficiency were usually greater from slurry applications in February than from those in October, but the timing of the application of FYM had a much smaller effect, compared with the timings of the application of slurry, on the utilization of N from manure by grass. Spring assessment of SMN was useful in quantifying available N residues from October slurry applications. Manure‐N recovery for all application timings was, on average, higher from the sandy loam than the shallow calcareous clay loam. The application of slurry to grass in early spring, at a rate of 150 kg total N ha^?1, with the addition of a supplementary 50 kg inorganic fertilizer‐N ha^?1, was the most suitable strategy for utilizing slurry‐N effectively and for supplying the N requirement for first‐cut silage.     

Effect of fertilizer nitrogen source and cattle slurry on herbage production and nitrogen utilization

A field plot experiment was carried out on an established grassland sward from 1983–88 inclusive to examine the effects of time of application, chemical form of nitrogen (N) and cattle slurry dry matter (DM) content on yield and efficiency of N use. Four forms of fertilizer N (a semi-organic fertilizer, a combined 2.1:1 (w/w) semi-organic/calcium ammonium nitrate (CAN) fertilizer, CAN and urea, each supplying 300 kg N ha^?1 year^?1, were applied with or without unseparated or separated cattle slurry at 93 and 73 g kg^?1 DM respectively, both supplying approximately 150 kg N ha^?1 year^?1. All fertilizers and slurries were applied in three equal dressings (February/March, May/June and July/August). The efficiency of use of fertilizer and slurry N was evaluated by measuring DM yield, N uptake and apparent recovery of N in herbage at all harvests during each growing season. Fertilizer N application significantly increased (P<0.001) the mean yields of herbage at each harvest in all years. The form of fertilizer N had no significant effect (P>0.05) on first harvest and total herbage yields, nor on N uptakes by herbage at the first harvest in any year. The performance of urea and of CAN was more variable at the second and third harvests relative to that of the semi-organic or combined 2.2:1 (w/w) semi-organic/CAN sources which had similar efficiencies of N use. Lower DM production was associated with reduced uptake of N. Values for mean overall apparent recovery of N ranged from 57.9 ± 2.67% for the semi-organic fertilizer to 50.2±3.05% for CAN. Unseparated cattle slurry and separated cattle slurry produced similar herbage yields and N responses that were lower and more variable than with fertilizer N. The overall mean apparent recovery of N from unseparated cattle slurry was 25.5 ± 5.03% compared to 5.0 ± 4.74% for separated cattle slurry. Efficiency of N use was highest with spring applications and least with mid-season applications. Recoveries ranged from ?29% for separated cattle slurry applied in June 1984 to 56% for unseparated and separated cattle slurry applied in February 1988 and June 1987 respectively. No interactions were recorded between cattle slurry and fertilizer N in terms of DM production or N uptake by herbage. The results of this study support the use of a fertilizer N source, selected on a least-cost basis, in combination with slurry to promote spring herbage production. For subsequent production, N should be supplied in fertilizer form only. The use of urea is risky under low rainfall conditions. Mechanical separation did not improve the efficiency of use of slurry N.     

The comparative performance of Holcus lanatus and Lolium perenne under sheep grazing in the Scottish uplands

An experiment was conducted over 3 years (1983-85) to assess the performance of Holcus lanatus German Commercial and cv. Massey Basyn compared with Lolium perenne cv. Perma on a gley soil under sheep grazing in the Scottish uplands. All grasses were sown together with Trifolium repens cv. Grasslands Huia. The swards were rotationally grazed at similar herbage allowances. During the first harvest year, the swards were grazed hard to a low mass (500 kg DM ha⁻¹). In the second harvest year, post-grazing herbage masses of 500 and 1000 kg DM ha⁻¹ were compared.
Perma ryegrass had a higher level of herbage production than both H. lanatus cultivars in the first and second harvest years after sowing and hence had a greater number of sheep grazing days. The ryegrass sward consisted of 25% more green sown grass (85 cf. 60%) but 12% less white clover (4 cf. 16%) compared with both H. lanatus cultivars in the establishment and first harvest years. The persistence of all three grasses was poor although ryegrass had a higher presence (36%) than either Massey Basyn (22%) or German Commercial (13%) at the end of the. second harvest year.
At a similar herbage allowance, there were no significant differences in the herbage intake and liveweight gain of sheep. Ryegrass had a higher organic matter digestibility and lower neutral and acid detergent fibre and lignin contents than either of the H. lanatus cultivars.
In the second harvest year, although herbage production was greater at the higher herbage mass, there was no difference in the proportion of sown grass.
It was concluded that ryegrass is a superior grass to H. lanatus on upland soils with high N status, moderate P status and a high pH.     

The influence of surface and sub-surface application methods for pig slurry on herbage yields and nitrogen recovery

Experiments were conducted on a grassland site at Wrest Park, Silsoe, Bedfordshire between 1987 and 1989, to compare herbage yields from slurry applied by deep and shallow injection, low trajectory and conventional vacuum tanker methods. Slurry application rates for all spreaders were calibrated at 86 ± 5 t ha^-1, an equivalent of c. 200 kg NH₄⁺-N ha^-1 applied in autumn or in spring.
As expected, herbage yields following spring applications were higher than from autumn applications, with average mineral fertilizer equivalents of 122 and 89 kg N ha^-1 respectively. Yields from the conventional and low trajectory spreaders showed no consistent differences. However, in both years, first cut yields from plots were significantly lower ( P < 0·05) where slurry had been injected than where surface applications had been used by an average of 0·7 t DM ha^-1. Subsequent cuts in 1988 demonstrated higher residual effects from injection so that annual total yields were similar from all slurry applications irrespective of spreader type.
Analysis of N content revealed high N levels in herbage from deep injection plots. Mean concentrations of N in the herbage, expressed as a percentage of the dry matter, were 1·43 for surface treatments and 1·79 for deep injection in 1988, and 1·84 for surface treatments, 2·13 for shallow injection and 2·68 for deep injection in 1989.     

The effect of strategic fertilizer nitrogen and date of primary harvest on the productivity of a perennial ryegrass/white clover sward

Four management systems involving different dates for first harvest (simulated grazing, early silage, late silage and hay) and two fertilizer N rates in spring (0 and 80 kg ha^-1) were imposed on a perennial ryegrass cv. Talbot/white clover cv. Blanca sward during 1981-82. In each year, annual total herbage DM was increased by spring application of N but white clover production and content in the total herbage were reduced; however, white clover, which was depressed in the harvests immediately after N application, recovered during the season to amounts and contents in the total herbage similar to those given no spring N.
Annual total herbage DM production increased as the date of primary harvest was delayed (935 to 1197 t ha^-1 over two years) but mean organic matter digestibility values for the same period decreased (0-769 to 0700). First-harvest production made up substantial proportions of the annual production in the conservation systems. White clover, as shown by its production and the amount of stolon present, was tolerant of conservation systems, especially with no applied N.
It is concluded that grass/white clover swards are suitable for management systems which involve cutting for conservation. The use of strategic spring N seems a viable option, but more knowledge of rates would be valuable since this experiment only compared 80 kg ha^-1 with no applied N.     

THE EFFECT OF FERTILIZER NITROGEN AND WHITE CLOVER ON HERBAGE PRODUCTION

The effect of a range of N rates on herbage production from grass/white-clover and grass swards was investigated in two long-term grazed experiments. The mixed sward yielded more than the grass swards over the range of fertilizer rates tested which were 0–120 lb N/ac (134.5 kg/ha), but the response of the mixed sward to fertilizer N was less than that of tbe grass sward. Tbis was due to the direct and indirect effect of clover, which was considerable with no fertilizer N but decreased witb increasing rates of application. However, clover was not completely suppressed by N at eitber site and contributed to berhage yields at all N rates. Rate and time of N application and the amount of clover in tbe sward affected tbe seasonal distribution of DM yields. The mid-season decline was less marked at bigb- tban at intermediate-levels of N supply.     

Implications of nitrogen fertilizer applications and extended grazing for the N economy of grassland

The response of swards which have been previously grazed to N fertilizer applied in early February was studied in two experiments in Northern Ireland. The effect of N fertilizer applied at a range of dates in autumn and spring on swards for out-of-season utilization was studied in a further experiment. Deep soil coring was also undertaken, subsequent to grazing with dairy cows, in grazed and protected areas in November and March to investigate the effect of out-of-season grazing on soil mineral N levels.
Dry-matter (DM) yield response to early spring N application in previously grazed swards was low, with no effect on DM yield in February or March. Progressively delaying N application (and commencement of herbage accumulation) in autumn from 8 September until 18 October reduced herbage availability in late autumn and early spring but increased leaf lamina content. The greater the amount of herbage accumulated to 1 December, the lower the tiller density in the following April.
N fertilizer had a greater impact on soil mineral N in spring than in late autumn/early winter, suggesting that fertilizer N was more prone to loss in the latter. Soil mineral N was not significantly affected by out-of-season grazing.
It is concluded that in well-fertilized, previously grazed swards response to N for out-of-season herbage is low and the probability for N loss is increased. Herbage quality will decline and the sward may be damaged if about 2 t DM ha⁻¹ or more of harvestable herbage accumulates for use in winter or in early spring.     

The effects of rate and timing of application of fertilizer nitrogen in late summer on herbage mass and chemical composition of perennial ryegrass swards over the winter period in Northern Ireland

A small‐plot experiment was carried out in Northern Ireland on a predominantly perennial ryegrass sward over the period July 1993 to March 1994 to investigate the effect of timing and rate of fertilizer nitrogen (N) application on herbage mass and its chemical composition over the winter period. Eighty treatment combinations, involving four N fertilizer application dates (28 July, 9 and 30 August and 20 September 1993), four rates of N fertilizer (0, 30, 60 and 90 kg N ha^?1) and five harvest dates (1 October, 1 November, 1 December 1993, 1 February and 1 March 1994), were replicated three times in a randomized block design experiment. N application increased herbage mass at each of the harvest dates, but in general there was a decrease in response to N with increasing rate of N and delay in time of application. Mean responses to N applications were 13·0, 11·5 and 9·5 kg DM kg^?1 N at 30, 60 and 90 kg N ha^?1 respectively. Delaying N application, which also reduced the length of the period of growth, reduced the mean response to N fertilizer from 14·3 to 7·4 kg DM kg^?1 N for N applied on 28 July and 20 September respectively. Increasing rate of N application increased the N concentration and reduced the dry‐matter (DM) content and water‐soluble carbohydrate (WSC) concentration of the herbage but had little effect on the acid‐detergent fibre (ADF) concentration. Delaying N application increased N concentration and reduced DM content of the herbage. The effect of date of N application on WSC concentration varied between harvests. A decrease in herbage mass occurred from November onwards which was associated with a decrease in the proportion of live leaf and stem material and an increase in the proportion of dead material in the sward. It is concluded that there is considerable potential to increase the herbage mass available for autumn/early winter grazing by applying up to 60 kg N ha^?1 in early September.     

A comparison of diploid and tetraploid perennial ryegrass and tetraploid ryegrass/white clover swards under continuous sheep stocking at controlled sward heights. 3. Sward characteristics and animal output, years 4–8

Results for years 4–8 of a long-term grazing experiment on swards of a diploid perennial ryegrass (Lolium perenne), var. Contender (D swards), a tetraploid ryegrass, var. Condesa (T swards) and Condesa with S184 white clover (Trifolium repens) (TC swards), direct sown in May 1987, are presented. The swards were continuously stocked with sheep from 1988 to 1990, as previously reported, and for a further 5 years, 1991–95, at a target sward surface height (SSH) of 4–6 cm. Control of sward height was successfully achieved by variable stocking, except in 1993 when paddocks were set stocked and the resulting mean SSH was 9·3 cm. Grass swards received on average 160 kg N ha^?1 year^?1; grass/clover swards were mainly not fertilized with N with the exception that they were given 30 kg N ha^?1 as a remedial mid-summer application during a period of low herbage mass on offer in 1994 and 1995. Mean white clover content of the swards fell from 18·2% of herbage dry-matter (DM) in 1992 to 8·5% in 1993, whereas stolon lengths fell from 120 to 58 m m^?2. A return to lower sward heights in 1994–95 resulted in an increase in white clover content to 12·8% by the final sampling in August 1995. Perennial ryegrass content of the grass swards remained high throughout (mean 96·7% in 1995). Perennial ryegrass tiller densities recorded in August 1991, 1993 and 1994 showed consistently significant (P < 0·001) sward differences (3-year mean 16 600, 13 700 and 10 100 perennial ryegrass tillers m^?2 for the D, T and TC swards). In 1994, the year after lax grazing, a low perennial ryegrass tiller density (9100 m^?2) and low white clover content (mean 4·3%) in the TC swards resulted in a much lower herbage bulk density than in the grass swards (April–July means 72, 94 and 44 kg OM ha^?1 cm^?1 for the D, T and TC swards). There was a consistent 40 g d^?1 increase in lamb liveweight gain on the TC swards over the T swards, except in 1994. In that year there was a reduction in lamb liveweight gain of 33 g d^?1 on the TC swards and a significant increase in ewe liveweight loss (117 g d^?1) associated with low herbage bulk density despite optimal sward height. Lamb output (kg liveweight ha^?1) on TC swards reflected white clover content, falling from a similar output to that produced from grass given 160 kg N ha^?1, at 18% white clover DM content, down to 60% of grass + N swards with around 5% clover. A 6% greater output from the T than the D swards was achieved mainly through higher stocking rate. The experiment demonstrated a rapid, loss in white clover under lax grazing, and showed that the relationship between performance and sward height is also dependent on herbage density. High lamb output from a grass/clover sward was only achieved when the clover content was maintained at 15–20% of the herbage DM.     

Digestion and nitrogen metabolism in beef cattle and in vitro rumen fermentation of autumn grass differing in fertilizer nitrogen application rate

Beef cattle partition dietary nitrogen (N) into meat or excrete it mainly in faeces and urine, which can contribute significantly to water and air pollution. The effects of two inorganic nitrogen (N) fertilizer application rates—15 (LN) or 80 (HN) kg N/ha—to Lolium perenne‐dominant swards in autumn, on herbage chemical composition, intake, digestion and N balance in beef cattle, and in vitro fermentation and methane production were studied. Four growing beef steers used in a 2 × 2 crossover design experiment were offered zero‐grazed grass harvested 21 days post‐N application between July and October. The same grasses were incubated in an eight‐vessel rumen simulation technique in a randomized complete block experiment. Grass dry‐matter (DM) concentration was 26 g/kg lower and crude protein (CP) concentration was 35 g/kg DM higher for HN compared to LN. There was no difference in herbage DM intake or in vivo DM digestibility between treatments. Nitrogen intake and the digestibility of N were higher for HN compared to LN. Total and urine N loss were 41 and 45 g/day greater, respectively, for HN compared to LN, but faecal N loss did not differ between treatments. The quantity of N retained and therefore N‐use efficiency did not differ between LN and HN (25% vs. 22%). In vitro rumen pH and ammonia‐N concentrations were higher for HN compared to LN, whereas volatile fatty acid concentrations and molar proportions did not differ between treatments. In vitro methane and total gas production were 0.9 mmol/day and 280 ml/day lower for HN than LN respectively. Under the particular conditions of this experiment, reducing fertilizer N application rate reduced total and urinary N excretion, which has potential environmental benefits; however, methane output measured in vitro was increased.     

Herbage and nitrogen yields,fixation and transfer by white clover to companion grasses in grazed swards under different rates of nitrogen fertilization

In grass–legume swards, biologically fixed nitrogen (N) from the legume can support the N requirements of the grass, but legume N fixation is suppressed by additional fertilizer N application. This study sought to identify a fertilizer N application rate that maximizes herbage and N yields, N fixation and apparent N transfer from white clover to companion grasses under intensive grazing at a site with high soil‐N status. During a 3‐year period (2011–2013), swards of perennial ryegrass and of perennial ryegrass–white clover, receiving up to 240 kg N ha^?1 year^?1, were compared using isotope dilution and N‐difference methods. The presence of white clover increased herbage and N yields by 12–44% and 26–72%, respectively. Applications of N fertilizer reduced sward white clover content, but the effect was less at below 120 kg N ha^?1. The proportion of N derived from the atmospheric N fixation was 25–70%. Nitrogen fixation ranged from 25 to 142 kg N ha^?1 measured using the isotope dilution method in 2012 and from 52 to 291 kg N ha^?1 using the N‐difference method across all years. Fertilizer N application reduced the percentage and yield of fixed N. Transfer of N from white clover to grass was not confirmed, but there was an increased N content in grass and soil‐N levels. Under intensive grazing, the maximum applied N rate that optimized herbage and N yields with minimal effect on white clover content and fixation rates was 60–120 kg N ha^?1.     

Effect of fertilizer nitrogen on six-year-old red clover/perennial grass swards

Three diploid red clover cultivars—Sabtoron, Violetta and Essex—and three tetraploid, Hungaropoly, Teroba and Red Head, were sown separately in pure culture and with each of three companion grasses: timothy (Aberystwyth S48), tall fescue (Aberystwyth S170) and perennial ryegrass (Aberystwyth S24).
The effects of fertilizer N on yield and on clover/grass ratio over a 2-year period (seventh and eighth harvest years) subsequent to 6 harvest years during which no N fertilizer was applied were investigated. The data for productivity and persistence have already been published (McBratney, 1981; 1984).
Application of fertilizer N increased DM yields in the eighth year. In this year, the highest yield, 11·9t ha^-1, averaged over the six clover cultivars, was given in association with tall fescue. Tall fescue contributed 90% of this yield. Clover content continued to decrease in all swards but the decrease was greatest in the swards receiving fertilizer N. The yield of clover DM averaged over the six cultivars under N treatment declined from 5·6t ha^-1 in the seventh year to only 0·4t ha^-1 in the eighth year.
The results from this trial demonstrate the potential of red clover sown either pure or in mixture with a suitable perennial grass, to maintain high output of quality herbage over a 6-year period without the aid of fertilizer N. They further demonstrate that following decline in red clover content, both herbage yield and quality may be restored by the application of N fertilizer, particularly where the clover was seeded with a highly productive companion grass.     

THE EFFECT OF FERTILIZER NITROGEN ON SILAGE FERMENTATION

Direct-cut grass which has been heavily fertilized often produces silage with a poor fermentation. An investigation was carried out to study the effect of rate and timing of N applications to herbage intended for direct-cut silage. A small but consistent increase in silage pH was recorded after high levels of N were applied 7–8 weeks before cutting. The application of additional N fertilizer 10–14 days before cutting the sward had an adverse effect on silage fermentation. Changes in herbage composition following different levels of applied N are outlined.     

THE EFFECT OF A RYEGRASS COMPANION GRASS AND THE VARIETY OF RED CLOVER ON THE PRODUCTIVITY OF RED-CLOVER SWARDS

An experiment was conducted to measure the effects of differrat ryegrass companion grasses and red-clover varieties on the productivity of red-clover swards. Three silage harvests per year were taken over a 2-year period. The addition of a companion grass increased total herbage yields; S24 perennial ryegrass gave the highest herbage yield over the two years, followed by Reveille perennial ryegrass. Because of lack of persistence, Tetila Italian ryegrass yielded poorly the second year. A companion grass had little effect on red-clover yields in the mixed swards but improved percentage digestibility of the OM of the total herbage and lowered the CP percentage. Its presence also reduced the ingress of unsown species. The variety of red clover used had little effect on total herbage yields or red-clover yields in the first harvest year. In the second year, Hungaropoly and Tilo persisted better and so gave higher total herbage yields and red-clover yields than Dorset Marl or Essex. The fall in total herbage yields from the first to the second year was entirely due to a fall in red-clover yield since yields of the ‘non-red clover’ fraction of the total herbage increased. A red-clover/grass sward may have advantages over a pure red-clover sward nutritionally, for silage-making and for its effect in diluting the oestrogenic activity of a pure clover sward. A major reappraisal of the role and potential of red-clover swards in the UK is warranted because of their many valuable attributes, particularly their ability to give high herbage yields of high nutritive value without the addition of fertilizer N. The improved persistency of some of the tetraploid varieties of red clover enhances the value of the plant.     

Slot-seeding investigations. 1. Effect of level of nitrogen fertilizer and row spacing on establishment, herbage growth and quality of perennial ryegrass

Three field experiments showed that perennial ryegrass can be successfully slot-seeded into an existing Festuca-Agrostis sward. Ryegrass survival was satisfactory in all cases but was improved on a low fertility site by the application of fertilizer N, P and K in the slot at sowing.
The effect of varying inter-row spacing from 37·2 to 15 cm was measured in a 3-year experiment. In year one, involving seven cuts, slot-seeding increased total herbage dry matter harvested by a mean value of 17%; inter-row distances of 22·2-30 cm gave the optimal combination of ryegrass + old sward herbage. Differences in yield between inter-row spacings declined in the subsequent two years, as the rows of ryegrass thickened. Ryegrass digestibility (measured only in year two, from six cuts) was higher than that of the old sward; total metabolizable energy harvested from 15-cm rows was 20% higher than that from unsown controls. Increasing N input from 200 to 400 kg ha^-1, starting in year two, only produced a significant increase in total herbage harvested in year three but proved effective in increasing the ryegrass contribution from the wider spaced rows.