Effect of dietary calcium and phosphorus concentrations on retention of these nutrients by caged layers

1. A 5 3 factorial experiment was carried out with caged White Leghorn hens with 5 concentrations of calcium (26.0, 29.0, 32.5, 36.0, 39.0 g/kg) and 3 concentrations of phosphorus (4.3, 5.0 and 6.0 g/kg) for 120 d. Variables observed were hen day egg production, food consumption, shell weight, shell weight per unit surface area (SWUSA) and egg content. 2. 36.0 g calcium (Ca) and 4.3 g phosphorus (P) kg were found to be the dietary concentrations that resulted in optimal hen day egg production, shell weight, SWUSA and egg content. 3. Lack of a significant interaction between the effects of calcium and phosphorus on production parameters showed that the dietary Ca: P ratio is not of great importance for the laying hen. 4. Absolute retentions of Ca and P were inversely related to percentage retentions. 5. A balance study of calcium and phosphorus also showed optima at 36.0 g Ca and 4.3 g P per kg. 6. It was inferred that 36.0 g Ca and 5.0 g total P per kg are the optimal concentrations in diets for caged layers in a tropical climate.     

Influence of dietary calcium and phosphorus on metabolism and production in laying hens

1. The effects of different dietary concentrations of calcium (24 to 56.9 g/kg) and phosphorus (4.5 to 14.2 g/kg) on production and some aspects of metabolism were studied in laying hens.

2. Treatments did not affect egg numbers, food consumption, conversion efficiency of food to egg, bodyweight gain or mortality.

3. Increasing dietary calcium (Ca) significantly increased plasma Ca and inorganic phosphorus (P), breaking strength at the radius and egg specific gravity and significantly decreased plasma alkaline phosphatase and egg weight.

4. Increasing dietary phosphorus increased plasma P and decreased egg specific gravity significantly.

5. Plasma Ca, P and alkaline phosphatase and radius breaking strength were suitable indices of the Ca status of the hens.     

饲粮钙磷水平对笼养北京白鸡蛋壳质量及产蛋性能的影响

<正> 笼养来航鸡蛋壳质量是尚未完全得到解决的问题。母鸡产蛋后期蛋重增大而沉积钙的能力有限,使蛋壳变薄容易破损。影响蛋壳质量的原因有:蛋鸡对沉积钙的能力,激素的分泌,饲粮钙磷水平与维生素D的含量,磷与钙在血液中的比例,以及钙磷的贮存与动员中互相制约的关系等。     

Effect of Energy and Protein on Performance,Egg Components,Egg Solids,Egg Quality,and Profits in Molted Hy-Line W-36 Hens

An experiment was conducted to determine the influence of dietary energy on performance, egg composition, egg solids, egg quality, and profits of Hy-Line W-36 hens fed different protein levels. The experiment was designed as a 4 × 3 factorial arrangement with 4 added dietary energy levels (0, 79, 158, and 238 kcal of ME/kg) and 3 protein levels (14.89, 16.06, and 17.38%). The basal diets of 17.38, 16.06, and 14.89% protein contained 2,751, 2,784, and 2,815 kcal of ME/kg, respectively. This study lasted 12 wk. Molted Hy-Line W-36 hens (n = 1,440) in phase 1 (70 wk of age) were randomly divided into 12 treatments (8 replicates of 15 hens per treatment). Protein had a significant effect on egg production, egg mass, feed intake, feed conversion, egg weight, percentage of egg shell components, yolk color, and yolk and albumen weight. As dietary energy increased from 0 to 238 kcal of ME/kg by addition of poultry oil, feed intake linearly decreased. Increasing dietary energy also significantly increased BW and egg yolk color. As dietary energy increased, percentage yolk solids increased at the 2 greater dietary protein levels, whereas egg-specific gravity linearly decreased at the 17.38% protein level. Increasing dietary energy and protein significantly improved feed conversion. Increasing protein intake significantly increased albumen and yolk weight but had no influence on yolk, albumen, or whole egg solids. Because feed ingredient and egg prices vary, there can be no fixed ideal dietary energy level for optimal profits during molt phase 1 (70 to 81 wk).     

The effects of phytase and root hydroalcoholic extract of Withania somnifera on productive performance and bone mineralisation of laying hens in the late phase of production

1. A 6-week study was conducted to investigate the effects of phytase and hydroalcoholic extract of Withania somnifera root (WS) on productive performance and bone mineralisation of laying hens in the late phase of production.

2. Diets were arranged factorially (3?×?2?×?2) and consisted of a positive control with adequate Ca (4·37%) and nonphytate P (NPP; 0·39%) and a negative control diet with Ca (4·06%) and NPP (0·36 %); three concentrations of Withania somnifera (0, 65 and 130?mg/kg diet); and two concentrations of microbial phytase (0 and 300 U/kg diet).

3. A total of 144 72-week-old Hy-Line W36 laying hens were randomly assigned to the 12 treatment groups. Each treatment was replicated 4 times (4 x 3 hens). Egg production and egg weight were recorded daily, while feed intake and egg quality traits were recorded every two weeks. Bone quality traits were evaluated at the end of experiment.

4. Withania somnifera supplementation increased egg production and lowered egg weight only in the second two weeks of the experiment. Addition of phytase significantly depressed specific gravity of the eggs for the entire experiment period. No dietary treatment effects were observed on egg shell thickness and yolk weight.

5. Withania somnifera at 130?mg/kg did not affect feed intake. The hens fed on the positive control diet had higher albumen weight than the negative control diet in the second two-week period. Supplementation of the positive control diet with 65?mg/kg Withania somnifera in the absence of phytase significantly improved shell weight compared with the negative control (5·779 vs. 5·273?g respectively).

6. Supplementing Withania somnifera significantly improved Ca and P retention in tibia bone. In addition, an increase in tibia bone P was observed with phytase supplementation. There were significant interactions between Withania somnifera content and phytase for tibia bone Ca and P.

7. The results of this experiment indicated that dietary Withania somnifera has beneficial effects on tibia bone Ca and P content, and phytase improved tibia bone P retention without adverse effects on productive performance.     

Evaluation of the dietary interaction of calcium and phosphorus in the high producing laying hen

1. In a 6 x 7 factorial experiment using 2688 22-week-old laying hens of the Lohmann-SL strain kept in cages (4 birds/cage), diets containing six calcium (20, 25, 30, 35, 40, 45 g calcium/kg) and seven phosphorus concentrations (3.2, 4.2, 5.2, 6.2, 7.2, 8.2, 16.2 g total phosphorus/kg (Pt)) were combined orthogonally. The resulting 42 treatments were replicated 8 times so that a replicate consisted of a double cage of 2 x 4 hens. The experiment lasted 40 weeks (10 x 28 days). 2. The experimental diets, based on maize and soyabean meals contained 11.5 MJ metabolisable energy/kg and 175 g/kg protein. Different dietary calcium and phosphorus contents were obtained by substituting oat hulls with limestone and dicalcium phosphate. 3. Mortality, egg production, egg weight, egg mass, food intake and food conversion efficiency were determined as well as the breaking strength, thickness of shells and the percentage of eggs with defective shells. 4. All responses measured were significantly influenced by the variance sources (calcium, phosphorus, interaction). Most of the production traits responded asymptotically to increasing dietary phosphorus concentration, the greatest increases or decreases generally being seen between 3.2 and 5.2 g Pt/kg. Further but weaker increases were seen between 5.2 and 8.2 or 16.2 g Pt/kg. 5. Increases in dietary calcium content always resulted in curvilinear responses. In all cases optimal effects were obtained with diets containing 25 g calcium/kg and the worst values at 45 g calcium/kg. The interaction between calcium and phosphorus was recognised by strong performance depressions and a high mortality at combinations of the lowest phosphorus concentration (3.2 g/kg) with high calcium contents (35 to 45 g/kg). These were largely offset by increasing dietary phosphorus. Thus, between 7.2 and 16.2 g Pt/kg and 25 and 45 g Ca/kg a plateau was formed where only small differences in egg production were observed. 6. From the three egg shell characteristics measured, breaking strength and shell thickness responded differently to the percentage of eggs with defective shells. While breaking strength and shell thickness were respectively negatively and positively influenced by increasing dietary phosphorus and calcium contents, both elements affected the proportion of eggs with defective shells.(ABSTRACT TRUNCATED AT 400 WORDS)     

Influence of Dietary Energy and Antibiotic on Performance,Egg Solids,and Egg Quality in Bovans White and Dekalb White Hens

A 4 × 2 × 2 factorial experiment with 4 dietary energy levels (2,776, 2,820, 2,864, and 2,908 kcal/kg) and 2 strains (Bovans White and Dekalb White) with and without Tylosin was conducted to determine the influence of dietary energy and antibiotic on performance, egg solids, and egg quality. The experiment lasted 10 wk. Bovans White hens (n = 768) and Dekalb White hens (n = 768) at 55 wk of age were randomly divided into 16 treatments (8 replicates of 12 birds per treatment). Bovans hens had significantly greater egg production and significantly lower egg weight, egg-specific gravity, and shell weight than Dekalb hens. Increasing dietary energy by the addition of poultry oil had no significant effect on performance, egg solids, or eggshell quality. An ideal dietary energy level for optimal performance could not be determined. There can be no fixed ideal dietary energy level for optimal profits, due to varying feed ingredient and egg price. Although Tylosin supplementation had no effect on performance or egg solids, Tylosin significantly reduced dirty eggs, resulting in a positive effect on egg quality.     

Effects of age at onset of production,light regime and dietary calcium on performance,eggshell traits,duodenal calbindin and cholecalciferol metabolism

1. Rate of production and shell thickness (ST) decreased, while body weight (BW), egg weight (EW) and percentage breakage increased progressively with age. Shell weight (SW) increased until 8 to 13 months of age and then decreased. 2. Early onset of production resulted in lower BW and EW at the onset of production, and lower pooled averages of BW, EW, SW and ST, as compared with late or medial onset of production. In 4 out of 5 trials, early onset did not result in the production of more eggs during the laying period. 3. Early onset of production is associated with physiological Ca deficiency as indicated by increases in kidney-1-hydroxylase and duodenal calbindin in early layers as compared with late layers. Early layers exhibited a more severe reduction in shell quality as the result of Ca deficiency as compared with late layers. 4. Feeding pullets with a prelaying diet containing 3.9% Ca did not affect unequivocally the performance or shell quality during the whole productive period, whether the birds started to lay early or late. The dietary treatment did not cause renal damage, as indicated by morphological examination and by plasma calcium and uric acid concentration.     

Effect of the manganese content in laying hen feed with different Ca and mineral levels on the egg shell quality and bone mineralization of hens]

Four experiments with 270, 44, 432 and 66800 Leghorn hens were carried out to investigate the influence of various Mn additions to diets differed in mineral or Ca contents on egg shell quality. The addition of 300 mg Mn/kg diet improved significantly egg shell breaking strength by 4 N over one year. The supply of 50-500 mg Mn/kg diet for 10-24 weeks of the second half of laying year did not influence the egg shell quality. Addition of mineral mixture or Ca grit to layer rations with adequate or higher Mn levels did not influence egg shell strength. High mineral content in a low manganese diet increased number of cracks by 3%. Strength, weight and ash content of tibia were significantly reduced by feeding a low mineral level. Addition of 50-150 mg Mn per kg low mineral diet normalized partially tibia stability in young hens. It was concluded that supplied dietary Manganese influences calcification positively only in young hens. High levels of Ca did not influence the effects of Mn. 50 mg Mn per kg layers mixture have been considered as an essential supply.     

饲粮不同代谢能、粗蛋白质水平对新杨绿壳蛋鸡产蛋后期生产性能、蛋品质及血清生化指标的影响

本试验旨在通过研究饲粮不同代谢能、粗蛋白质水平对新杨绿壳蛋鸡产蛋后期(44~56周龄)生产性能、蛋品质及血清生化指标的影响,得出其产蛋后期饲粮代谢能和粗蛋白质的适宜配比。选取768羽体重相近的43周龄末新杨绿壳蛋鸡,随机分为6组,每组4个重复,每个重复32羽。采用2×3因子(代谢能水平为10.87和11.08 MJ/kg,粗蛋白质水平为15.00%、15.50%和16.00%)设计试验,共设计6种饲粮。试验预试期1周,正试期12周。结果表明:1)饲粮不同代谢能、粗蛋白质水平对绿壳蛋鸡产蛋后期产蛋率和料蛋比有显著影响(P0.05),饲粮代谢能和粗蛋白质水平在产蛋率上表现出极显著的交互作用(P0.01),其中,高能低蛋组(代谢能为11.08 MJ/kg,粗蛋白质为15.00%)产蛋率最高,料蛋比最低。2)饲粮较高粗蛋白质水平(16.00%)能显著提高绿壳蛋鸡产蛋后期鸡蛋的哈氏单位(P0.05);饲粮较高代谢能水平(11.08 MJ/kg)能显著提高绿壳蛋鸡产蛋后期鸡蛋的蛋黄颜色(P0.05);饲粮代谢能和粗蛋白质水平在蛋品质上未表现出显著的交互作用(P0.05)。3)高能低蛋组绿壳蛋鸡血清尿素氮含量最低;饲粮较高代谢能水平(11.08 MJ/kg)能显著降低绿壳蛋鸡血清总胆固醇含量(P0.05)。综上,在本试验条件下,饲粮代谢能、粗蛋白质水平分别为11.08 MJ/kg和15.00%时,更有利于绿壳蛋鸡产蛋后期生产性能的发挥和蛋品质的改善。     

Effect of excess dietary threonine on laying performance,egg quality,serum free amino acids,and digestive enzymes activities of laying hens during the postpeak period

A total of 640 layer hens, 56 wk of age, were allocated to 4 dietary treatments groups, each of which included 5 replicates of 32 hens. Graded levels ofl-Thr were added to the basal diet at 0.0 (control), 1.0, 2.0, and 3.0 g/kg; determined Thr values were 5.7, 6.5, 7.4, and 8.7 g/kg of diet, respectively. In the 53-d feeding trial, egg production increased significantly due to dietaryl-Thr supplementation. Hens fed 2.0 g of Thr/kg of diet had significantly higher egg production than the control diet. Supplementing the diet withl-Thr did not affect egg weight or feed intake, but egg mass increased significantly. Also, FCR was improved significantly and the best FCR was exhibited by hens receiving 2.0 g of Thr/kg of diet. Feeding laying hens a diet containing 2.0 g ofl-Thr/kg of diet improved Haugh units linearly; however, egg shell thickness decreased linearly as the level of dietary Thr increased, and the lowest value was observed for hens fed 3.0 g of Thr/kg of diet. Serum free Thr increased linearly as supplemental Thr increased, but serum free Ile, Phe, and Tyr decreased. No differences attributable tol-Thr were found among groups in the activity of digestive enzymes (trypsin and amylase) in the mucosal homogenates of the jejunum. The activity of α-alkaline phosphatase in the jejunum was analyzed as a marker of mucosal enterocyte maturation and no differences due tol-Thr supplementation were noted. In conclusion, feeding laying hens from 56 to 64 wk of agel-Thr up to 2.0 g/kg can improve laying performance and Haugh units.     

Protein and energy self-selection by turkey hens: reproductive performance

1. The influence of protein and energy self-selection on the reproductive performance of turkey hens was investigated. Large White turkey hens were fed on either a complete diet (185 g CP/kg, 11.30 MJ ME/kg) or were provided with a choice between a high protein-low energy diet (348 g CP/kg, 7.74 MJ ME/kg) and a high energy-low protein diet (81 g CP/kg, 13.39 MJ ME/kg). 2. Egg production, egg weight, and the fertility and hatchability of eggs produced were not significantly affected by the dietary treatments (P greater than 0.05). 3. While energy intake was not significantly different between groups, protein consumption decreased by approximately 40% (P less than or equal to 0.001) among self-selecting hens compared to hens fed on the complete diet. This resulted in a 10% (P less than or equal to 0.05) reduction in the food intake in the self-selecting birds.     

Nutritional and environmental factors involved in egg shell quality of laying hens.

1. Two experiments were conducted to assess the effects of lighting regime, dietary calcium source and sodium bicarbonate on production variables and egg shell quality of White Leghorn hens. In both experiments, hens were assigned to one of three lighting programmes that provided evening, morning or intermittent (midnight) lighting supplements in addition to natural daylight. Experimental diets used in the first study were formulated to contain (1) ground oyster shell flour, (2) limestone flour, or (3) and (4) the same +2/3 of the calcium source as hen-size oyster shell grit. The same 4 diets plus those containing hen-size limestone or hen-size limestone and oyster shells were used in experiment II. Additionally, diets in the first experiment contained either 0 or 10 g/kg sodium bicarbonate. 2. Hen-day egg production and food consumption were not affected by any of the experimental treatments. Hens fed on oyster shell diets or exposed to intermittent lighting regimes laid eggs of the highest specific gravity. Shell quality, as measured by specific gravity, was not affected by the addition of dietary sodium bicarbonate. 3. As expected, elevated temperatures (greater than 32 degrees C) significantly reduced egg shell quality. However, this effect was variable particularly in experiment II which used younger hens. 4. The shell quality of eggs from hens exposed to intermittent lighting in experiment II was significantly higher in each of the 4 sampling periods: morning (08.00-12.00), afternoon (12.00-16.00), evening (16.00-20.00) and night (20.00-08.00). 5. It is suggested that midnight lighting programmes provide a means of supporting egg shell quality of older laying hens during the summer months without a significant reduction in egg production.     

Dietary phosphorus supply, egg-shell deposition and plasma inorganic phosphorus in laying hens

1. In 2 experiments the effects of dietary phosphorus on relationships between plasma inorganic phosphorus concentration (Pi), shell and egg production and depletion states were measured in brown laying hens. 2. In a 12-week experiment dietary phosphorus concentrations from conventionally deficient (1.6 g non-phytate-phosphorus (PNP)/kg) to moderate excess (3.9 g PNP/kg) had little effect on egg and shell production, although there was evidence that plasma Pi concentration, when not influenced strongly by shell formation, reflected dietary phosphorus content. 3. Among birds at each dietary phosphorus concentration there was a negative linear relationship between shell weight of early eggs in the sequence and plasma Pi concentration. The relationship was apparently not affected by dietary phosphorus concentration. 4. Continued feeding of the deficient diet to 61 weeks of age did not have effects on body weight, egg and shell production, other than those associated with age, but plasma Pi and bone measurements indicated marginal phosphorus depletion. 5. In another experiment excessive dietary phosphorus (11.9 g PNP/kg) fed in a cross-over design caused small adverse effects on shell production, increased food intake and body weight and increased plasma Pi content, while there was no relationship between shell weight and plasma Pi concentration. 6. The results are consistent with an indirect effect of plasma phosphorus accumulation on shell formation, probably via an inhibitory effect on skeletal calcium release, in addition to any effect of excess dietary phosphorus on intestinal calcium availability. 7. Phosphorus requirement and status in the laying hen are complicated by the failure to recognise the contribution of digestible phytate-phosphorus to the available phosphorus supply.     

Effect of reduced energy, protein and entire substitution of inorganic phosphorus by phytase on performance and bone mineralisation of laying hens

1. The objective of this study was to evaluate the effects of total removal of dietary inorganic phosphorus and reduced energy and protein, without and with phytase supplementation, on the performance, egg quality and bone composition of laying hens. 2. Lohmann pink-shell hens were randomly assigned at 56 weeks of age to 5 treatments for 20 weeks as follows: (1) a positive control (PC) with 155 g CP/kg, 11·09 MJ ME/kg, calcium (Ca) 3·40% and non-phytic phosphorus (NPP) 0·26%, (2) a negative control (NC1) diet based on PC diet with Ca decreased to 3·30% and NPP to 0·14%, (3) NC2 diet was formulated on the basis of NC1 diet with 152·7 g CP/kg, 10·90 MJ/kg, (4) NC1 and (5) NC2 supplemented with phytase (300 FTU/kg) each. 3. Feed intake, hen-day or hen-housed egg production, egg number per hen-housed, and final body weight were depressed with NC1 and NC2 diets, but restored by phytase inclusion. There were no significant differences between the dietary treatments for feed conversion efficiency, rates of cracked and broken eggs, egg-shell thickness or egg-shell strength. Mortality was significantly increased by NC2 diet without phytase. Tibia ash was significantly decreased by both NC1 and NC2 diets. Bone strength, and Ca and P contents in tibia ash were significantly increased by phytase inclusion in the NC1 diet. 4. In conclusion, the NC1 and NC2 diets significantly depressed performance and tibia quality, but the addition of phytase (300 FTU/kg) significantly improved performance and tibia integrity.     

Influence of high dietary vitamin E supplementation on egg production and plasma characteristics in hens subjected to heat stress

1. The effects of different dietary concentrations of vitamin E (alpha-tocopherol acetate) were investigated in 2 experiments on laying hens exposed to chronic heat stress at 32C. 2. In the first experiment, egg production and plasma concentrations of calcium and egg yolk precursors were measured in 24 hens before, during and after a stress period of one week and fed on diets containing 10 or 500 mg vitamin E/kg. 3. In the second, larger experiment, egg production and food intake were measured in 300 hens housed in 2 temperature-controlled rooms and fed on diets containing 10, 125 or 500 mg vitamin E/kg. Birds in room 1 were stressed from 24 to 28 weeks of age and those in room 2 from 32 to 36 weeks. 4. In experiment 1, egg production and egg weight were significantly higher (72.6 vs 51.2%, P < 0.05 and 66.6 vs 63.1 g, P < 0.005 respectively) during and after the period of stress in the group given 500 mg vitamin E/kg. Plasma concentrations of calcium, vitellogenin (zinc) and VLDL (triglyceride) were also higher in this group. 5. In experiment 2, egg production was significantly higher (65.4 vs 56.2%, P < 0.05) during and after the period of heat stress in birds in room 1 fed on the diet containing 500 mg vitamin E/kg. Egg production was also higher (49.9% vs 44.7%) on this treatment during the stress period in room 2, though the difference was not significant (P < 0.10). Egg weight and food intake were unaffected by treatment in either room. 6. It is concluded that dietary supplementation with extra vitamin E can, at least in part, alleviate the adverse effects of chronic heat stress in laying hens, perhaps by maintaining the supply of egg precursors in plasma.     

日粮中添加辣诺素对海兰褐蛋鸡产蛋性能和蛋品质的影响

本试验旨在研究日粮中添加辣诺素（辣木和诺丽果的提取物）对蛋鸡生产性能及蛋品质的影响。将产蛋高峰期（22周龄）的海兰褐商品蛋鸡540只随机分为6个处理,每个处理6个重复,每个重复15只鸡。负对照组（NC）饲喂基础日粮,正对照组（PC）饲喂添加50 mg/kg杆菌肽锌的基础日粮,4个试验组分别饲喂添加0.25、0.50、0.75和1.00 g/kg辣诺素（LNS）的基础日粮。试验期24周。结果显示：①日粮处理对蛋鸡试验前期和后期生产性能均无显著影响（P>0.05）,试验后期添加0.50和0.75 g/kg辣诺素有改善料蛋比的趋势（P=0.087）;日粮处理对试验前期和后期鸡蛋蛋形指数、蛋壳强度、蛋壳厚度、蛋黄重和哈氏单位均无显著影响（P>0.05）,但日粮中添加杆菌肽锌和辣诺素有提高蛋壳强度的趋势（P=0.062）,其中以添加0.75g/kg辣诺素组鸡蛋强度最高。②与正、负对照组相比,添加辣诺素显著增加了生蛋黄和熟蛋黄的罗氏蛋黄色比率（P<0.05）,显著降低了蛋黄胆固醇的含量（0.25 LNS组除外）（P<0.05）。③与负对照组相比,添加0.50 g/kg辣诺素显著提高了鸡蛋总黄酮、二十二碳六烯酸（DHA）、卵磷脂、亚麻酸和亚油酸含量（P<0.05）。综合以上试验结果,辣诺素作为饲料添加剂具有提高鸡蛋营养品质的功能,考虑到添加成本,产蛋鸡日粮中添加0.25~0.50 g/kg为宜。     

Effects of dietary cottonseed meal and iron-treated cottonseed meal in different laying hen genotypes

The effects of dietary screw-pressed cottonseed meal (CSM) and iron-treated CSM on laying performance and discolourations in eggs were examined in a range of hen genotypes. In experiment 1, six genotypes, obtained at point-of-lay from various sources, were fed on a non-CSM diet, a diet with 300 g CSM/kg, and a diet containing iron-treated CSM at 300 g/kg. In experiment 2, two of these genotypes were reared together from day-old and were fed from 10 to 18 weeks on a non-CSM diet or a diet containing iron-treated CSM at 250 g/kg. They were then fed on a non-CSM layer diet or a diet containing iron-treated CSM at 300 g/kg, in a 2 x 2 x 2 factorial design that also examined the effects of the rearing diet. 2. The effects on food intakes and egg production of including CSM and iron-treated CSM in layer diets depended on the genotype of the hens. The strongest interaction between breed and diet was on food intake, the breed Hubbard Golden Comet (HGC) being the least tolerant of CSM and iron-treated CSM. 3. Inclusion of iron-treated CSM in the rearer diet to supply approximately 70% of the dietary protein had no adverse effects on growth or age at first egg. Food intake and egg production between 18 and 26 weeks were affected by the iron-treated CSM layer diet, but there were no carry-over effects attributable to the rearing diets. 4. Genotype was not a factor in the development of the gossypol-related brown yolk discolouration in fresh or warm-stored eggs of hens fed on a CSM-based diet containing 197 mg free gossypol/kg and 52 mg cyclopropenoid fatty acids (CPFA)/kg (experiment 1). 5. In both experiments, the susceptibility of eggs to the CPFA-related cold storage effects depended on the genotype of the hen, eggs from hens of the HCG breed being more affected than those of ISA hens. 6. Treatment of CSM with crystalline ferrous sulphate heptahydrate, at a 4:1 weight ratio of iron to free gossypol, prevented brown yolk discolourations in all genotypes tested, as assessed by subjecting egg yolks to atmospheres of ammonia, and cold storage of eggs.     

The influence of dietary boron supplementation on egg quality and serum calcium, inorganic phosphorus, magnesium levels and alkaline phosphatase activity in laying hens

This study was performed to investigate the influence of dietary boron (B) supplementation on egg production, interior and exterior egg quality and serum parameters related to egg formation in laying hens. Two hundred and twenty four, 18 week old, Hyline Brown 98 strain hens were assigned to 7 groups with 4 replicates. After 10 days of adaptation the hens were fed for 8 weeks on diets supplemented with 0, 5, 10, 50, 100, 200 and 400 mg/kg (diet) B (H(3)BO(3)). Live weight, feed consumption and egg production were reduced in the 400 mg/kg B supplemented group. Increases were determined in serum Ca, inorganic P and Mg levels and serum ALP activity was not influenced by supplemental B. Boron changed the interior and exterior quality of eggs. In particular, high level of B supplementation reduced live weight, feed consumption and egg production and changed some blood metabolites.     

Lack of response of laying hens to relative humidity at high ambient temperature

1. The effects of relative humidity (rh=40% to 70%) at high ambient temperature (Ta) on the performance of laying hens at different ages (8 to 10 months, Trial 1; and 16 to 18 months, Trial 2) was evaluated. Laying hens were exposed to 25 degrees C (control) for 3 weeks and thereafter acclimated for 1 week to 35 degrees C and 4 different rh. 2. Body weight declined significantly in young and older hens exposed to 60% or 70% and 70% rh, respectively: Food intake declined with increasing Ta, except in the case of older hens exposed to 60% rh, for which it remained relatively constant. Water consumption, however, increased with increasing Ta but the increase was significant in young hens exposed to 70% rh only. 3. Egg production was not affected by the changes in Ta. However, a decrease in egg production was observed in older hens exposed to 60% rh. 4. Egg weight (EW), shell weight (SW) and shell thickness (ST) were significantly reduced by exposure to elevated Ta, whereas % breakage significantly increased. In young hens, a response to rh was exhibited in ST which was significantly higher in hens exposed to the low rh (40% to 45%) than in those exposed to the highest rh (70% to 75%). 5. It can be concluded that Ta is the main environmental factor affecting young and older laying hens while the effect of rh is minor.