采精频率对德州驴精液品质的影响

为探究采精频率对成年种公驴精液品质的影响,对东阿国家黑毛驴繁育中心34头种公驴的采精状况及生产数据进行跟踪与统计分析,并将数据分为4组,A组(每3天采精1次)、B组(每4天采精1次)、C组(每5天采精1次)和D组(每10天采精1次)。结果表明:D组的总精液量显著低于其他各组(P0.05),C组的总精液量显著低于A组(P0.05)和B组(P0.05);C组精液的精子密度与D组间无显著性差异(P0.05),但显著高于A组(P0.05)和B组(P0.05);D组精液的原精活力和冻后活力均显著低于其他各组(P0.05),但A、B、C三组间差异并不显著(P0.05);D组精子的复苏率显著低于其他各组(P0.05),B组与C组间差异不显著(P0.05),但均显著低于A组(P0.05);采精频率与冻后活力、复苏率之间均呈显著负相关(P0.05)。结论:种公驴采精频率为每3天采精1次时,可以获得稳定的精液品质。     

日粮中添加l-精氨酸对公犬精液品质、生殖器超声参数及血清睾酮的影响

为了探究日粮中添加l-精氨酸对公犬精液品质、生殖器超声参数及血清睾酮的影响,试验从沈阳市某警犬基地选取体重相近的4～5岁健康史宾格公犬20只,随机分为4组(n=5),分别为试验A组、试验B组、试验C组和对照组,试验A组、试验B组和试验C组分别按体重每天在日粮中添加10,15,20 mg/kg l-精氨酸,对照组日粮中不添加l-精氨酸,试验期为30 d。于试验第1,10,20,30天分别对各组的精液品质(精液量、精液、密度精子、活力精子活率)、生殖器超声参数(睾丸宽度、睾丸深度和附睾尾部厚度)及血清睾酮质量浓度进行检测。结果表明：试验第1,10天,各指标各组间均差异不显著(P>0.05)。试验第20天,试验A组、试验B组和试验C组的精液密度、精子活力显著高于对照组(P<0.05),试验B组的精液密度显著高于试验A组和试验C组(P<0.05);试验B组和试验C组左右两侧睾丸宽度和睾丸深度显著高于对照组和试验A组(P<0.05)。试验第30天,试验A组、试验B组和试验C组的精液密度、精子活力显著高于对照组(P<0.05),试验B组的精液密度和精子活力显著高于试验...     

品种、年龄及采精频率对犬精液品质和精子形态的影响

为探究品种、年龄及采精频率对犬精液品质和精子形态的影响,本实验选取沈阳某警犬基地1～9岁健康德国牧羊犬和马里努阿犬各30头,按照品种(德国牧羊犬和马里努阿犬)、年龄(1～3岁、4～6岁和7～9岁)、采精频率(2、4、6、8 d和10 d)设置不同组别。用计算机辅助精子分析法(CASA)测定精液体积、精子密度、精子活力、精子活率等精液品质指标,采用伊红Y染色结合CASA测定精子头部长度、头部宽度、顶体比例、尾部中段长度等精子形态指标。结果显示：年龄对精液品质的影响较品种、采精频率对精液品质的影响更大,7～9岁年龄段各项精液品质指标出现下降(P<0.05);4～6岁年龄段的精液体积、精子密度、精子活力均优于1～3岁及7～9岁;采精频率为每间隔2、4d和10d时,精液体积、精子密度、精子活力和精子活率下降,采精频率为6 d或8 d对精液品质无影响;两犬种之间精液品质差异不显著。德国牧羊犬与马里努阿犬精子中段长度分别为11.35、8.74μm左右,存在显著差异;两犬种精子头部长度、宽度和顶体比例无显著差异;同一品种不同年龄段犬的精子形态差异不显著。在制定犬繁殖育种计划时,应将公犬年龄作为...     

不同保存时间和容器对海兰褐父母代种鸡精液品质的影响

试验旨在研究不同保存时间和容器对蛋用型种公鸡精液品质的影响。采集50周龄海兰褐父母代种公鸡的精液,用生理盐水稀释200倍,试验分12组,每组设3个重复,精液盛放容器分别是塑料和玻璃集精管,保存时间设置6个水平:15、30、45、60、90、120min。结果显示:两种集精管存放的精液,保存15min和30min精子活力最高、畸形率最低,15min略好于30min(P>0.05);15、30min两组精子活力显著或极显著高于45、60、90和120min组(P<0.05或P<0.01),畸形率显著低于45、60、90、120min组(P<0.05),保存120min组精液品质最差。相同保存时间下,玻璃集精管精子活力显著高于塑料集精管(P<0.05),畸形率低于塑料集精管(P<0.05)。研究表明:采精后精液保存时间以不超过30min为宜;玻璃集精管保存精液效果优于塑料集精管。     

采精员和猪舍环境参数对杜洛克公猪精液品质的影响

【目的】研究采精员、猪舍温湿度、氨气浓度和光照强度对种公猪精液品质的影响,以提高猪精液品质。【方法】选取347头(580±48) d的杜洛克公猪,收集2021年11月至2022年2月共2 966条精液测定记录,采精员做好每天公猪的采精记录,猪舍温湿度、氨气浓度和光照强度参数于每天上午09:00进行测定。使用R软件对采精员记录、环境参数和精液性状进行关联分析。【结果】(1)采精员对精液品质具有显著影响(P<0.05),8号采精员采集的精液精子活力最高、精子畸形率最低。(2)猪舍环境温度为22～25℃时,精子活力显著高于18.5～22℃(P<0.05),精子畸形率显著低于18.5～22℃(P<0.05)。(3)相对湿度在70%～77%时精子活力最高、精子畸形率最低,与相对湿度在46%～60%时差异显著(P<0.05),与相对湿度在60%～70%时无显著差异(P>0.05),相对湿度在60%～70%时精子活力、精子畸形率和总精子数都处于较高水平。(4)随着氨气浓度降低,精子活力和总精子数呈现上升趋势、精子畸形率呈下降趋势,氨气浓度2～4 ppm与5～7.3 pp...     

种公鸡日粮中添加蜂花粉的效用研究

研究蜂花粉对人工采精中采不出精液的公鸡的繁殖性能及内分泌机能的影响。结果表明:日粮中添加2%的蜂花粉,能显著改善公鸡的精液量、精子活力、精子密度和有效精子数(P<0.05),对精子畸形率影响不大(P>0.05),有效缓解血浆T水平的下降(P<0.05),对E2水平无明显影响;与对照组相比,添加蜂花粉后试验组血浆T3含量升高(P<0.05)、T4含量(P<0.05)和皮质醇含量(P>0.05)下降,说明蜂花粉改善了热应激公鸡的甲状腺功能和肾上腺功能。     

采精频率对不同品种公猪精液品质的影响

为了探讨采精频率对杜洛克、大白、长白种公猪精液品质的影响，并确定不同品种种公猪的最佳采精频率，试验选取12月龄体重相近的健康杜洛克、大白、长白种公猪各6头，分5个不同采精频率阶段采精，即第1阶段(A组)为1次/1 d,第2阶段(B组)为1次/2 d,第3阶段(C组)为1次/3 d,第4阶段(D组)为1次/4 d,第5阶段(E组)为1次/5 d,每个阶段采精3次，一个阶段采精完成后，公猪休息一周，然后开始下一个阶段采精，采精完成后对每份精液的精液量及精子密度、活率和畸形率指标进行统计分析。结果表明：三个品种种公猪精液量及精子密度和活率随采精频率的降低均总体呈升高趋势，精子畸形率随着采精频率的降低呈现下降趋势；杜洛克种公猪C组的精液量、精子密度、精子活率较佳，E组精子畸形率最低，但与B、C、D三组精子畸形率差异不显著(P>0.05);大白种公猪B组精液量较佳，D组精子密度较佳，C组精子活率较佳，E组精子畸形率最低，与D组精子畸形率差异不显著(P>0.05);长白种公猪C组精液量较佳，D组精子密度较佳，C组精子活率较佳，E组精子畸形率最低，与D组精子畸形率差异不显著(P>0...     

采精频率对长白种公猪精液品质的影响

为探讨采精频率对种公猪精液品质造成的影响，本试验采用计算机辅助精液分析仪检测了四川某种猪繁育场的10头长白种公猪在不同采精频率下（1、2、3、4、5次/周）的精液，分别其对精子活力、密度、精液量、精子结构变化和精子运动性能等指标的影响。结果表明：采精频率为3次/周时，精子密度最高，与采精频率为4次/周的精子密度差异不显著（P> 0.05），与采精频率为1、2和5次/周时的差异显著（P> 0.05）；采精频率为3次/周时精子活力和活率最高，与采精频率为5次/周时的活力与活率差异显著（P> 0.05）；精子总数在采精频率为3次/周时最多，精液量未呈现出规律性变化，但在采精频率为1次/周时最多，与采精频率为5次/周时的差异显著（P> 0.05）。综上所述，采精频率为3次/周时精液品质最佳；采精频率为4次/周和2次/周时精液品质次之。     

采精频率对长白种公猪精液品质的影响

为了研究采精频率对成年长白种公猪精液品质的影响。试验通过对6头成年长白种公猪进行五个不同阶段的采精试验,分别为A阶段(采精1次/2 d),B阶段(采精1次/3 d),C阶段(采精1次/4 d),D阶段(采精1次/5 d),E阶段(采精1次/6 d)。研究结果表明:(1)采精频率为A组时,其精液采精量显著低于B组、C组、D组、E组(P0.05),而B组采精量虽低于C组、D组,但差异不显著(P0.05),所以成年种公猪精液采精量的最佳采精频率为B组;(2)采精频率为D组时,其精液精子密度与E组差异不显著(P0.05),但显著高于A组、B组、C组(P0.05);(3)采精频率为C组时,C组精液的精子活率与D组、E组差异不显著(P0.05),但与A组、B组差异显著(P0.05);(4)当采精频率为A组、B组时,其精液中精子畸形率均达到20%以上,而E组、D组的精子畸形率均在5%左右,且E组、D组间差异不显著(P0.05),而C组精子畸形率在15%左右,虽低于20%,但与E组、D组差异显著(P0.05);(5)采精频率与总精液量、活率、密度之间都呈现极显著的负相关性(P0.01),与精子畸形率呈现极显著的正相关(P0.01)。     

注射口蹄疫疫苗对种公牛鲜精品质的影响

本研究比较了经免疫注射口蹄疫疫苗后两个品种共13头种公牛的鲜精精液品质。结果表明,荷斯坦牛和西门塔尔牛免疫期的精液量和精液密度指标无明显变化(P>0.05);活力指标显著降低(P<0.05);精子畸形率显著增加(P<0.05)。尾部畸形增加是影响精子直线运动和冲力的主要原因。     

海藻糖、二甲乙酰胺对公猪精液冷冻保存效果的研究

采用5% 二甲乙酰胺(DMA)(V/V)完全替代甘油,比较乳糖、海藻糖对精液冷冻保存效果的影响。结果表明,海藻糖显著提高了冷冻——解冻后精子成活力(49.32%±1.52%)与顶体完整性 (47.33%±1.16%)(P<0.05)。然后利用海藻糖替代乳糖,评价不同浓度的DMA对公猪精液冷冻保存的影响。结果表明,当DMA添加量为4%时,解冻后精子活率、成活力、顶体完整率分别为(45.17±0.56)%、(50.33±0.67)%、(48.30±1.44)%,均显著高于3% DMA、6% DMA添加组(P<0.05),精子活率显著高于5% DMA添加组(P<0.05),但精子成活力、顶体完整性与其差异不显著(P>0.05)。因此,当利用海藻糖作为冷冻保存基础稀释液,DMA最适添加量为4%。     

红景天粗多糖中基分对猪精子冻后生化指标及活力的影响

试验将采自5头杜洛克（2～4岁）猪精液（2次/周）冷冻解冻于含有红景天粗多糖中基分（Middle Components of Rhocliola Polysacchoride,MCRP）的稀释液中,应用计算机辅助精子分析系统对猪精子运动特征进行了评价,其它参数用常规精子评价方法进行评估,分析MCRP对猪精子冻后生化指标及活力的影响。结果表明,MCRP在稀释液中的有效浓度范围为4.0～6.0mg/L,当MCRP浓度为6mg/L时,其各项指标均显著高于其它各组（P〈0.05）,而且冷冻解冻后,MCRP添加于稀释液I的精子质量显著优于MCRP添加到稀释液II的精子质量（P〈0.05）。冷冻解冻液中MCRP的浓度与解冻后解冻液中的谷胱甘肽、精子线粒体活性、精子低渗肿胀系数呈显著正相关（P〈0.05）,而与解冻液中的丙二醛浓度呈显著负相关（r=-0.982,P〈0.05）。猪精液冷冻保护液中添加MCRP能够有效地抵抗由于冷冻产生的活性氧自由基对精子的伤害,从而提高精子冻后质量,但是MCRP抑制活性氧自由基的具体成分仍需要进一步的研究。     

Post-thaw viability of european bison (Bison bonasus) semen frozen with extenders containing egg yolk or lipids of plant origin and examined with a heterologous in vitro fertilization assay.

Basic characteristics of European bison (Bison bonasus) semen were described and the efficacies of two extenders-Triladyl, containing egg yolk, and a synthetic extender, containing soybean lipids-were tested for semen cryopreservation. Seven ejaculates were collected by electroejaculation from a 10-yr-old, European bison bull. Each ejaculate was diluted at 37 degrees C to a final concentration of 200 x 10(6) sperm/ml with Triladyl or the synthetic extender. Extended semen samples were frozen according to a standard bull semen freezing protocol. After 2 wk of storage, one straw from each extender and ejaculate was thawed, and postthaw quality was evaluated by individual sperm motility and movement rate, numbers of sperm morphologic abnormalities and intact acrosomes, functional integrity of the sperm membranes determined by hypoosmotic swelling test (HOST), viability (live-dead, eosin-nigrosin stain), and a heterologous in vitro sperm penetration assay (SPA). A total of 600 in vitro-matured bovine oocytes were inseminated with 1 X 10(6) spermatozoa of Holstein semen frozen-thawed in Triladyl (control) or of European bison semen frozen in Triladyl or the synthetic extender. Nuclear status of the oocytes was determined after 18 h of sperm-oocyte coincubation. Extender had no effect on any evaluated parameters of semen after dilution and cooling (4 hr at 5 degrees C) or in postthaw individual motility, quality of movement, and sperm morphology. However, significantly (P < 0.05) higher numbers of spermatozoa with intact acrosomes, intact membranes (HOST), and viable sperm (P < 0.01) were in semen frozen in Triladyl than in the synthetic extender. Mean values for heterologous SPA for bull (control) and for bison semen frozen in the synthetic extender were very much alike-63.3+/-10.6% and 63.1 +/- 15.9%, respectively; bison semen frozen in Triladyl was lower, 43.0+/-24.2% but not significantly different. Cumulative results from a variety of viability assays of diluted/cooled and frozen-thawed semen, including the heterologous SPA, suggest that European bison semen can be successfully frozen in both extenders tested in this study.     

Relationships among age, body weight, scrotal circumference, semen quality and peripheral testosterone and estradiol concentrations in pubertal and postpubertal Holstein bulls

In the present study, the correlations among age, body weight, scrotal circumference (SC), semen quality and peripheral testosterone and estradiol-17beta (E(2)) concentrations were investigated in pubertal (n=5) and postpubertal (n=7) groups of Holstein bulls over a 6 week period. There were significant positive correlations (P<0.01) among age, body weight and SC in both groups, and similar significant correlations between sperm motility and SC in pubertal bulls (P<0.01) and between sperm concentration and SC in postpubertal bulls (P<0.05). The sperm motility after collection (P<0.05) and after freezing and thawing (P<0.01) of the postpubertal bulls correlated positively with the E(2) concentration. Estrogen may be important for the function of postpubertal bull testes, in which it may regulate spermatozoa motility in vivo.     

Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry

The use of frozen semen in the swine industry is limited by problems with viability and fertility compared with liquid semen. Part of the reduction in sperm motility and fertility associated with cryopreservation may be due to oxidative damage from excessive or inappropriate formation of reactive oxygen species (ROS). Chemiluminescence measurements of ROS are not possible in live cells and are problematic because of poor specificity. An alternative approach, flow cytometry, was developed to identify viable boar sperm containing ROS utilizing the dyes hydroethidine and 2', 7'-dichlorodihydrofluorescein diacetate as oxidizable substrates and impermeant DNA dyes to exclude dead sperm. The percentage of sperm with high mitochondrial transmembrane potential was determined by flow cytometry using the mitochondrial probe 5, 5', 6, 6'-tetrachloro-1, 1', 3, 3'-tetraethylbenzimidazolylcarbocyanine iodide with propidium iodide staining to exclude nonviable cells. Sperm were incubated with and without ROS generators and free radical scavengers. Basal ROS formation was low (less than 4%) and did not differ (P = 0.26) between viable fresh and frozen-thawed boar sperm. In addition, fresh and frozen-thawed viable sperm were equally susceptible (P = 0.20) to intracellular formation of ROS produced by xanthine/xanthine oxidase (94.4 and 87.9% of sperm, respectively). Menadione increased (P < 0.05) ROS formation, decreased (P < 0.05) JC-1-aggregate fluorescence intensity, and decreased (P < 0.05) motion variables by 25 to 60%. The mechanism of inhibition of motility by ROS formation may be related to a decrease in mitochondrial charge potential below a critical threshold. Catalase and superoxide dismutase treatment in the presence of xanthine/xanthine oxidase indicated that hydrogen peroxide was the primary intracellular ROS measured. Further, catalase, but not superoxide dismutase, was capable of attenuating ROS-induced inhibition of motility. Whereas basal intracellular hydrogen peroxide formation was low in viable fresh and frozen-thawed boar sperm, both were quite susceptible to external sources of hydrogen peroxide.     

二甲基甲酰胺对猪精液冷冻保存效果的影响

用二甲基甲酰胺(DMF)完全替代甘油,比较不同平衡时间和不同DMF添加量对猪精液冷冻保护效果的影响。结果表明,DMF能完全替代甘油,获得较好的冷冻保护效果。最佳平衡时间为90 min,解冻后精子活力为(44.57±0.72)%,显著高于对照组和其他组(P0.05)。当DMF添加量为5%时,冻后精子活力、活率、线粒体活性、顶体完整率和质膜完整率分别为(49.91±0.39)%(、46.51±0.26)%、(47.51±0.52)%(、49.84±0.56)%、(46.30±1.61)%,均显著高于2%、3%、6%DMF添加组(P0.05),但与4%DMF添加组相比,冻后精子活力、活率和质膜完整率差异不显著(P0.05)。本试验结果表明,DMF最适添加量为5%。     

Effects of dietary supplementation with an organic source of selenium on characteristics of semen quality and in vitro fertility in boars

Semen characteristics in boars fed organic or inorganic sources of Se were assessed in 3 experiments. Crossbred boars were randomly assigned at weaning to 1 of 3 dietary treatments: I) basal diets with no supplemental Se (control), II) basal diets with 0.3 mg/kg of supplemental Se from an organic source (Sel-Plex, Alltech Inc., Nicholasville, KY), and III) basal diets supplemented with 0.3 mg/kg of supplemental Se from sodium selenite (Premium Selenium 270, North American Nutrition Co. Inc., Lewisburg, OH). For Exp. 1, semen was collected from boars (n = 10/dietary treatment) on 5 consecutive days at 15 mo of age. Effects of treatment × day were detected for the proportions of progressively motile (P = 0.02) and rapidly moving (P = 0.03) spermatozoa, and measures of sperm velocity, including path velocity of the smoothed cell path (P = 0.05) and average velocity measured in a straight line from the beginning to the end of the track (P = 0.05). Negative effects of day of semen collection on sperm motility were least pronounced in boars fed Sel-Plex. Experiment 2 was conducted when boars were 17 mo of age, and semen was collected (n = 10 boars/dietary treatment), diluted in commercially available extenders, and stored at 18°C for 9 d. Effects of treatment × day were detected for percentages of motile (P = 0.01) and static (P = 0.01) spermatozoa, amplitude of lateral head displacement (P = 0.02), frequency with which the sperm track crossed the sperm path (P = 0.04), straightness (P = 0.01), and average size of all sperm heads (P = 0.03). In general, sperm cells from boars fed Sel-Plex were better able to maintain motility during liquid storage compared with boars fed sodium selenite. For Exp. 3, semen was collected from boars (n = 6/dietary treatment) at 23 mo of age, and spermatozoa were evaluated at d 1 and 8 after semen collection using in vitro fertilization procedures. There was a tendency for an effect (P = 0.11) of dietary treatment on fertilization rate with Sel-Plex-fed boars having the greatest value (70.7%). The results of this study suggest that there are positive effects of dietary supplementation with Sel-Plex on boar semen characteristics and that organic Se supplementation may help ameliorate the negative effects of semen storage on characteristics of sperm motility.     

DNA fragmentation kinetics and postthaw motility of flow cytometric-sorted white-tailed deer sperm

This study examined DNA damage and postthaw motility of white-tailed deer sperm (n = 28) before and after sex selection and conventional sorting using MoFlo XDP SX flow cytometry. Semen samples from the same individuals were treated in 4 different ways: 1) chilled-extended sperm samples (without glycerol); 2) cryopreserved conventional samples, samples directly cryopreserved after the addition of extenders; 3) cryopreserved conventionally sorted samples, sorted samples to remove the dead sperm subpopulation; and 4) cryopreserved sex-sorted samples; sorted samples to remove the dead sperm subpopulation and separation of X- and Y-chromosome-bearing sperm. In all the cases (n = 6), conventional samples showed decreased postthaw motilities (43 ± 26%) when compared with X-sorted samples (59 ± 20%; P < 0.05) and Y-sorted samples (54 ± 20%; P > 0.05). The DNA fragmentation baseline was <5% for frozen-thawed conventional samples, but even less after sex sorting and conventional sorting: 2.4 and 1.7%, respectively. On the other hand, conventional samples showed greater (P < 0.05) DNA fragmentation than the sex-sorted sperm (n = 6) at 96 h (average of 4.8 ± 4.5% and 5.3 ± 4%, respectively). Conventionally sorted samples (n = 8) did not have greater (P > 0.05) DNA fragmentation when compared with the sex-sorted samples. Fragmentation of DNA on X-chromosome and Y-chromosome-bearing sorted sperm were not significantly different (n = 10, P > 0.05) after 96 h (2.6 ± 3.6% and 2.2 ± 0.5%, respectively). Future research should be implemented for examining the fertilizing potential of sex-sorted white-tailed deer sperm (e.g., AI fertility trials).     

Fertility of Mares Inseminated With Frozen-Thawed Semen Processed by Single Layer Centrifugation Through a Colloid

The aim of this study was to determine whether there was an increase in pregnancy rates when frozen-thawed stallion semen was processed by single layer centrifugation (SLC) through a colloid before insemination. In addition, changes in semen parameters, including motility, were determined before and after SLC. Twenty light-horse mares (aged 3-16 years) and one Thoroughbred stallion (aged 16 years) having average fertility with fresh and cooled semen (>50% per cycle) and displaying a postthaw motility of >35% were used. Control mares were inseminated using 4- × 0.5-mL straws (200 × 10⁶/mL) of frozen-thawed semen. Treatment mares were inseminated with 4 × 0.5 mL of frozen-thawed semen after processing by SLC. Pregnancy rates were compared using Fisher exact test, and continuous parameters were evaluated by a Student t test. The pregnancy rates at day 14 were not different for the mares inseminated with control versus SLC-processed semen, despite the difference in sperm number (171 × 10⁶ ± 21, 59 × 10⁶ ± 25 progressively motile sperm). After frozen-thawed semen was processed by SLC, the percentage progressively motile sperm improved (P < .05), and SLC processing resulted in a 21.8% recovery of spermatozoa. In summary, centrifugation of frozen-thawed semen through a single layer of colloid increased the percentage of motile spermatozoa, but did not improve pregnancy rates after deep horn insemination.     

维生素C对荷斯坦公牛X精子冻后品质的影响

试验旨在研究维生素C（VC）对荷斯坦公牛性控精液冻后品质的的影响。在精液稀释液中分别添加0,2.0,4.0,6.0,8.0 mg/mL的VC进行试验。将装有X精子的细管冻精解冻（37.5 ℃,30 s）后分析0～3 h精子活率及0～4 h质膜完整率和顶体完整率的变化情况。结果表明,当添加量为6.0 mg/mL时,解冻后0 h精子活率与对照无差异显著,解冻后1、2、3 h精子活率与对照组差异显著（P<0.05）。当VC的添加量为4.0 mg/mL时,解冻后0 h时X精子质膜完整率显著高于对照组(P<0.05),解冻后1 h与对照相比差异不显著（P>0.05）,但数值依然高于对照组。解冻后存放2、3、4 h与对照组相比差异显著。添加量为6.0 mg/mL时,解冻后0 h时X精子顶体完整率显著高于对照组(P<0.05),解冻后1、3 h精子顶体完整率与对照组相比差异显著（P<0.05）,2、4 h与对照相比差异不显著（P>0.05）,但顶体完整率高于对照组。在冷冻精液稀释液中VC的适宜添加量为4～6 mg/mL,此浓度下可有效提高荷斯坦公牛X精子解冻后活率、质膜完整率及顶体完整率。