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1.
Japanese flounder juveniles (initial body weight: 7.12 ± 0.02 g) were fed three diets containing 0, 120 and 200 g/kg of carbohydrates (C0, C120 and C200, respectively) for 10 weeks. Results showed that higher dietary carbohydrate intake enabled further deposition of glycogen and lipids in liver and muscle. The mRNA levels of glucokinase (gck), phosphofructokinase (pfkl) and hexokinase (HK) activity involved in glycolysis were significantly up‐regulated in C120 (p < .05) but showed no further up‐regulation except for gck in C200. Besides, the gluconeogenic phosphoenolpyruvate carboxykinase 1 (pck1) mRNA levels were down‐regulated significantly in fish fed the C120 (p < .05). However, further increase in dietary carbohydrate levels increased the mRNA levels and activities of enzymes involved in gluconeogenesis and lipolysis (p < .05). Additionally, plasma glucose remained unchanged in C120 (p > .05) but significantly increased in C200 group (p < .05). In conclusion, Japanese flounder was able to use carbohydrates efficiently through regulation of glucose and lipid metabolism when dietary carbohydrate was not higher than 120 g/kg, while 200 g/kg of dietary carbohydrate caused the deregulation of glucose homoeostasis.  相似文献   

2.
A 10‐week feeding trial was conducted to evaluate the growth performance, glucose transport and metabolism of Chinese soft‐shelled turtles (Pelodiscus sinensis) exposure to graded levels of dietary starch (0.52%, 7.43%, 14.74%, 22.99% and 31.38%). The 360 turtles (initial body weight, 12.94 ± 0.50 g) with 12 replicates were randomly assigned to five experimental diets. The highest weight gain and specific growth rate (SGR) were observed in 7.43% group and the lowest in 31.38% group. The protein efficiency ratio, whole‐body lipid contents, hepatic glycogen contents and the 4‐hr postprandial plasma glucose levels were significantly increased with the increment of starch levels (p < .05). In contrast, the daily feed intake and feed conversion ration were significantly declined (p < .05). The mRNA levels of glucose transporter 2, glucokinase, pyruvate kinase, malic enzyme and acetyl‐CoA carboxylase alpha genes in the liver significantly increased as the increase in starch levels at 4‐hr and 24‐hr post feeding (p < .05). No significant differences were observed in the expression of gluconeogenesis genes at each time point (p > .05). These results suggested that dietary addition of starch up‐regulated hepatic glycolysis, glycogenesis and lipogenesis genes expression, but the deficient response of gluconeogenesis to dietary starch might be part of the causes limited the starch utilization. Based on the secondary polynomial regression of SGR, y = ?0.0011x2 + 0.028x + 1.63 (R2 = 0.9292), the 12.73% inclusion level of dietary starch was recommended in juvenile turtles.  相似文献   

3.
A 12‐week feeding trial was conducted to determine a suitable carbohydrate source and form for Pangasianodon hypophthalmus. Ten isoenergetic, isonitrogenous and isolipidic diets, including five carbohydrate sources (dextrin, potato starch, wheat starch, corn starch and sago starch) and two carbohydrate forms (raw and cooked), were formulated. Results showed that specific growth rate, weight gain percentage, whole‐body lipid and 6‐phosphogluconate dehydrogenase enzyme activity of fish fed with cooked carbohydrates were significantly lower (p < .05) than those fed with raw carbohydrates (p < .05). Similarly, the fatty acid synthase enzyme activity of fish fed with the cooked form of carbohydrates was significantly lower (p < .05) than that fed with the raw form of carbohydrates except fish fed with cooked potato starch. The blood glucose level and malic enzyme activity of fish fed with cooked carbohydrate were significantly (p < .05) higher than those fed with raw carbohydrate. In summary, this study showed that cooked starch is an unsuitable dietary carbohydrate form for Pangasianodon hypophthalmus fingerlings. The raw forms of dextrin starch and potato are suitable dietary carbohydrate sources for further scientific studies on the carbohydrate utilization of P. hypophthalmus.  相似文献   

4.
5.
Juvenile brook trout were fed on six isonitrogenous and isolipidic diets, containing graded levels of gelatinized corn starch (50, 100, 150, 200, 250, 300 g/kg diet) for 81 days. Cellulose was used to compensate carbohydrate loss. The weight gain, specific growth rate and protein efficiency ratio increased as the dietary carbohydrate level increased from 50 to 150 g/kg, but decreased thereafter (p < .05). Quadratic regression analysis revealed that the optimum dietary carbohydrate level was in the range of 187.1–194.1 g/kg. In addition, serum alanine transaminase and aspartate transaminase activities, as well as glucose content, were highest in the group fed on 300 g/kg carbohydrate (p < .05). The hepatic malondialdehyde level increased with dietary carbohydrate levels (p < .05). The hepatic lysozyme activity increased as dietary carbohydrate level increased from 50 to 150 g/kg and decreased thereafter (p < .05). The overall survival rate after hypoxia challenge (45 min; dissolved oxygen content: 2.2 mg L?1) decreased with dietary carbohydrate levels (p < .05). Taken together, these results suggested that optimal level of dietary carbohydrate could improve growth performance. However, excessive dietary carbohydrate intake (> 250 g/kg) may decrease innate immunity status, increase oxidative stress and reduce resistance to hypoxia stress in brook trout.  相似文献   

6.
The growth performance, body composition, fillet fatty acid content, serum hepatic enzymes and postprandial changes in serum lipid metabolism of hybrid sturgeon (70.8 ± 0.5 g) were investigated to determine the effects of total replacement of 80 g kg‐1 fish oil (diet A) with linseed oil (diet B) and soybean oil (diet C), respectively. No significant differences in weight gain rate and specific growth ratio were observed among all fish groups (p > .05). Diet A fish group had the highest, but diet B fish group had the lowest feed intake (p < .05). Feed efficiency of fish fed diet A was significantly lower than the other two fish groups (p < .05). Fish fed diet A had the lowest fillet and liver lipid contents (< .05). Serum lactate dehydrogenase, alanine aminotransferase and aspartate aminotransferase activities of fish fed diet A were significantly lower than those of fish fed diets B and C (< .05). The contents of linoleic acid (C18:2n6) and linolenic acid (C18:3n3) in fillets showed a significantly positive linear correlation with the diets. Serum glucose and non‐esterified fatty acid were just affected by the time point (< .05). The lipid source, time point and interaction of both factors had significant effects on serum triglyceride, high‐density lipoprotein cholesterol and low‐density lipoprotein cholesterol (< .05). Serum total cholesterol was only affected by interaction of time point and lipid source (< .05), and ketone body was not affected by lipid source, time point or interaction of both factors (> .05). In summary, total replacement of fish oil with linseed oil or soybean oil had no significant adverse effects on hybrid sturgeon growth during 84‐day period, and linoleic acid and linolenic acid in fillet were modified by dietary treatments.  相似文献   

7.
An 8‐week growth trial was conducted to evaluate effects of dietary oil sources on growth, enzymes activity and genes expression levels related to lipid metabolism of hybrid grouper (♀Epinephelus fuscoguttatus × ♂E. lanceolatu) juveniles. Seven iso‐lipid (97 g/kg of dry matter) and iso‐protein (503.5 g/kg of dry matter) experimental diets were formulated containing 50 g/kg fish oil (FO; acting as controls) or various vegetable oils (VOs): corn oil (CO), sunflower oil (SO), tea oil (TO), olive oil (OO), rice oil (RO) and mixed oil (MO; comprising equal amounts of these oils). Each diet was fed to triplicate groups of 40 fish for per repetition (15.09 ± 0.01 g) for 56 days. The results show that (a) alternative dietary oils had no significant effects on final weight compared with control group (p > .05); (b) compared with FO group, VOs significantly changed the contents of serum lipoproteins, cholesterol, triglycerides and the activity of liver lipid‐metabolizing enzymes (p < .05); (c) CO group had the least effect on the serum lipoproteins, triglycerides and cholesterol of grouper compared with control; the activity of liver lipid‐metabolizing enzymes in RO and control group was the closest; (d) the mRNA levels of Δ6 Fatty acid desaturase (Δ6Fad), hormone‐sensitive lipase (HSL) and lipoprotein lipase (LPL) were not significantly effected by lipid sources, but CO, TO, OO and MO significantly down‐regulated the expression of fatty acid synthetase (FAS) mRNA level in liver, while RO opposite (p < .05); (e) vegetable oil significantly up‐regulated peroxisome proliferator‐activated receptor α (PPARα) and peroxisome proliferator‐activated receptor β (PPARβ) mRNA levels, while TO and RO down‐regulated peroxisome proliferator‐activated receptor γ (PPARγ) mRNA levels (p < .05); and 6) MO significantly increased the mRNA levels of heart‐type fatty acid‐binding protein (H‐FABP) and adipocyte‐type fatty acid‐binding protein (A‐FABP) (p < .05), while other VOs had no effect on them (p > .05). In conclusion, dietary substitution of FO by VO in diet affected lipid metabolism of grouper, which may be regulated by PPARs.  相似文献   

8.
An 8‐week trial was conducted to determine the effects of total replacement of 12.9% fish oil (FO) with soybean oil (SBO), peanut oil (PNO), sunflower seed oil (SFSO), corn oil (CO) and canola oil (CNO) on growth performance, health status and fillet fatty acid composition of hybrid sturgeon (194.28 ± 0.14 g). Compared to the FO group, dietary SBO decreased growth performance (p < .05), increased serum glucose and hepatic lipid content (p < .05). No obvious adverse effects on growth performance and health status were observed in PNO, SFSO and CO groups (p > 0.05). The fish fed with CNO had increased growth performance (p < .05), reduced serum ALT, AST, LDL‐C (p < .05) and enhanced serum GSH‐Px, T‐AOC, and LZM, MPO, C4 (p < .05). The contents of C18:1n9, C18:2n6, and ∑n‐3 PUFA and ∑n‐6 PUFA in fillets showed a positive linear correlation with the diets (p < .05). In summary, PNO, SFSO and CO are probable alternative lipid sources to fully replace FO. Hybrid sturgeon prefers to use CNO as a lipid source with improved growth performance and health status. The fillet fatty acid composition mirrors the dietary fatty acid composition.  相似文献   

9.
A 95‐day feeding trial was conducted to determine the effects of n‐3 highly unsaturated fatty acids (n‐3 HUFA) on the growth, antioxidation and lipid metabolism in grass carp (Ctenopharyngodon idellus). Two isonitrogenous and isolipidic diets were formulated with either lard oil (LO) or fish oil (FO) as the main lipid source. The results showed that the intraperitoneal fat (IPF) ratio was significantly lower (P < 0.05) in FO group. The concentration of n‐3 HUFA in muscle, hepatopancreas and IPF was significantly higher in FO group (P < 0.05). The serum low‐density lipoprotein (LDL) content was significantly lower (P < 0.05), and glucose (GLU) content was significantly higher (P < 0.05) in FO group. The serum total superoxide dismutase (T‐SOD) activity was significantly higher (P < 0.05) in FO group, consistent with the serum malondialdehyde (MDA) content. The gene expression of IPF fatty acid synthase (FAS), acetyl‐CoA carboxylase (ACC), sterol regulatory element‐binding protein (SREBP‐1) and peroxisome proliferator‐activated receptor γ (PPARγ) was significantly lower (P < 0.05) and that of peroxisome proliferator‐activated receptor α (PPARα) was significantly higher (P < 0.05) in FO group compared with LO group. Similar trends were found in the hepatopancreas, except for PPARγ. It is suggested that n‐3 HUFA could inhibit lipid accumulation in grass carp by affecting the expression of lipid‐metabolism‐related genes.  相似文献   

10.
11.
A 60‐day experiment was carried out to investigate dietary starch levels on growth performance, hepatic glucose metabolism and liver histology of largemouth bass, Micropterus salmoides. Fish (initial weight 22.00 ± 0.02 g) were fed five graded levels of dietary corn starch (0, 50, 100, 150 and 200 g/kg). Fish fed low (0 and 50 g/kg) dietary starch showed significantly higher weight gain than other groups (p < .05). Liver lipid and glycogen accumulations were induced when dietary starch higher than 100 g/kg. After 20 days of feeding, hexokinase activity and mRNA expression were decreased in fish fed dietary starch higher than 150 g/kg (p < .05) and the pyruvate kinase showed the opposite tendency. Insulin receptor 1 (irs1), glucagon‐like peptide‐1 receptor and glucose transport protein 2 (glut2) mRNA expression were decreased with the increasing dietary starch after 10 days of feeding (p < .05). These results indicated gluconeogenesis was depressed and β‐oxidation was enhanced in response to high dietary starch, while the glycolysis was inhibited and endocrine system was impaired when fish fed high dietary starch; then, glucose homeostasis was disturbed and finally led to the glucose intolerance of largemouth bass.  相似文献   

12.
A‐56 days feeding trial was conducted to evaluate the growth performance, feed utilization, biochemical composition, antioxidant status, lipid metabolism and immunity parameters of Chu's croaker Nibea coibor fed diets supplemented with different levels of conjugated linoleic acid (CLA): 0% (control), 0.5%, 1%, 1.5% and 2%. Each diet was randomly assigned to triplicate groups of 25 fish (initial body weight: 12.8 ± 0.1 g) in 15 floating cage. Weight gain (WG) and specific growth rate (SGR) were not significantly affected. 2% of dietary CLA led to significant lower lipid content in both whole body and liver (< .05). Muscle lipid content was significantly lower in fish fed 1.5% and 2% CLA (< .05). Saturated fatty acid (SFA) increased while monounsaturated fatty acid (MUFA) decreased with increasing dietary CLA in tissues (< .05). Increasing CLA significantly promoted hepatic lipase (HL), lipoprotein lipase (LPL), serum lysozyme (LSZ) and immunoglobulin M (IgM) while decreased serum triglyceride (TG) in both liver and serum (< .05). Liver Superoxide dismutase (SOD), catalase (CAT) and total antioxidant capacity (t‐AOC) increased while malondialdehyde (MDA) decreased (< .05). Our study shown that Chu's croaker can successfully incorporate CLA in tissues up to 2% dietary lipid without growth inhibition, and that CLA increased fish quality due to the increased parameters of lipid metabolism, specific immunity, hepatic antioxidant and CLA accumulation, and the reduction of tissue fat deposition.  相似文献   

13.
An 8‐week feeding trial was performed to examine the potential of total replacement of fish meal with animal by‐products with or without enzymatic components in juvenile genetically improved farmed tilapia (GIFT) diets, Oreochromis niloticus, (mean initial weight, 19.71 ± 0.28 g), reared in RAS system. Five isonitrogenous and isoenergetic diets were formulated with fish meal for the diets with total replacement of the isonitrogenous amounts of enzymatic fish meal (E‐FM), chicken liver meal (CLM), enzymatic chicken liver meal (E‐CLM), dried porcine solubles (DPS) and enzymatic dried porcine solubles (E‐DPS) respectively. The results indicated that the weight gain rate (WGR) and specific growth rate (SGR) in the FM group were significantly higher than the E‐FM and E‐CLM groups (p < .05), but the specific growth rate (SGR) in the E‐CLM group lower than the E‐FM group. The feed conversion ratio (FCR) in FM and CLM was significantly lower than the E‐FM, E‐CLM and E‐DPS groups (p < .05), and no significant difference was observed among the FM, CLM and DPS groups (p > .05). The values of the protein retention efficiency (PRE) and protein efficiency ratio (PER) in FM, CLM and DPS groups were significantly higher than the E‐CLM group (p < .05). The values of feed intake ratio (FIR) in FM and CLM groups were significantly lower than the E‐FM, E‐CLM and E‐DPS groups (p < .05). There was no significant difference in the whole body contents of moisture, crude lipid and crude ash among the FM and E‐CLM groups (p > .05). The whole body contents of crude protein in the FM group were significantly higher than the E‐FM, CLM, E‐CLM DPS and E‐DPS groups (p < .05). The apparent digestibility coefficients (ADC) of dry matter, crude protein and crude lipid in the FM, E‐FM, E‐CLM and E‐DPS groups were not significantly different (p > .05). The serum glucose(GLU), total cholesterol (TCHO) and triglycerides (TG), or the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not affected by the different treatments (p > .05). There was no significant difference in serum total protein (TP) or albumin (ALB) and between the FM group and other groups (p > .05). There was no significant difference in the serum urea nitrogen (BUN) content in the FM, CLM, E‐CLM and DPS groups (p > .05). Fish fed with the CLM and DPS diets had significantly lower hepatic GHR1, IGF1 and IGF2 gene mRNA expression levels than in fish fed the FM diet (p < .05). The present data showed that 6% of dietary fish meal can be totally replaced by the chicken liver meal and dried porcine solubles with no effect on the growth performance of GIFT strain tilapia.  相似文献   

14.
This study aimed to investigate the optimum dietary carbohydrate/lipid (CHO/L) ratio for fingerling blunt snout bream Megalobrama amblycephala (average initial weight: 6.61 ± 0.03 g). Six isonitrogenous and isoenergetic diets were formulated to contain various CHO/L ratios ranging from 1.62 to 24.20. Each diet was tested in four replicates for 10 weeks. Specific growth rate (SGR), feed conversion ratio and nitrogen and energy retention all improved significantly (< 0.05) as dietary CHO/L ratios decreased from 24.20 to 5.64 but showed little difference (> 0.05) as dietary CHO/L ratios ranged from 2.45 to 5.64. Hepatosomatic index increased significantly (< 0.05) as dietary CHO/L ratios increased, whereas intraperitoneal fat ratio showed an opposite trend (< 0.05). Opposite to moisture content, lipid content of whole body and carcass all increased significantly (< 0.05) as dietary CHO/L ratios decreased. Liver lipid content showed little difference (> 0.05) among all the treatments, while liver glycogen content increased significantly (< 0.05) with increasing CHO/L ratios. High dietary carbohydrate enhanced the activities of liver hexokinase, pyruvate kinase and glucose‐6‐phosphate dehydrogenase but did not induce hyperglycaemia. Based on the second‐order polynomial regression analysis of SGR, the optimal dietary carbohydrate and lipid contents for fingerling blunt snout bream were 291.7 and 81.4 g kg−1, respectively, with a corresponding dietary CHO/L ratio of 3.58.  相似文献   

15.
Phosphoenolpyruvate carboxykinase (PEPCK) is known to exist as mitochondrial (PEPCK‐M) and cytosolic isoforms (PEPCK‐C). They are involved in glucose production and play vital roles in regulating glucose homeostasis. This study aimed to (1) clone and characterize PEPCK‐C cDNA from golden pompano fish (Trachinotus ovatus), which are known to have poor utilization of dietary carbohydrates, and (2) analyze the regulation of its expression by varying dietary carbohydrate‐to‐lipid ratios and nutritional status. A full length golden pompano PEPCK‐C cDNA fragment of 2,652 bp was cloned, which contains an open reading frame of 1,875 bp encoding 624 amino acids and shows a high homology to cobia (Rachycentron canadum) PEPCK‐C sequence (92% similarity). The analysis of tissue distributions of PEPCK‐C mRNA showed that a high abundance of PEPCK‐C was expressed in golden pompano liver, followed by kidney; while lower expression levels were found in the heart and intestine. In liver, PEPCK is under the regulation of dietary composition and nutritional status at the enzymatic and molecular levels. Our results indicate that PEPCK‐C plays a modulating role in the adaptation of hepatic gluconeogenesis to different nutrient conditions. Thus, the absence of molecular inhibition of gluconeogenic PEPCK gene expression in rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata), which can at least partially account for their poor utilization of dietary carbohydrate, is specific to these species and is not observed in golden pompano. Further studies that examine, at the enzymatic and molecular levels, other key enzymes involved in hepatic glucose metabolism are necessary to understand the poor utilization of carbohydrates in golden pompano.  相似文献   

16.
A 90‐day trial investigated the effect of dietary carbohydrate level on growth performance, body composition, serum physiological responses and hepatic antioxidant abilities of a herbivorous fish, Wuchang bream (Megalobrama amblycephala) and a carnivorous fish, black carp (Mylopharyngodon piceus Richardson, 1846). For each species, two isonitrogenous and isolipidic semi‐purified diets (28.6% crude protein and 5.3% crude lipid for Wuchang bream) were formulated to contain 30.4% and 52.9% carbohydrate (WB‐NCHO and WB‐HCHO); Two isonitrogenous and isolipidic semi‐purified diets (32.6% crude protein and 5.3% crude lipid for black carp) were formulated to contain 20.5% and 40.5% carbohydrate (BC‐NCHO and BC‐HCHO). It was shown that, compared to Group WB‐NCHO, Wuchang bream in Group WB‐HCHO had lower weight gain rate, specific growth rate, serum total protein, hepatic superoxide dismutase activity and total antioxidative capacity, while higher feed conversion ratio, serum triglyceride and cortisol levels (< 0.05). Compared to Group BC‐NCHO, black carp in Group BC‐HCHO had lower final weight, weight gain rate, specific growth rate, protein efficiency ratio, serum alkaline alkaline phosphatase activity, serum total protein, hepatic superoxide dismutase activity and total antioxidative capacity, while higher feed conversion ratio, whole body crude lipid, serum triglyceride, cholesterol, glutamic‐oxaloacetic transaminase activity and cortisol levels (< 0.05). The results of this study suggested that Wuchang bream had better capacity of utilizing dietary carbohydrate than that of black carp, while high levels of dietary carbohydrate might not only be detrimental to growth performance and feed utilization but also the immune response and health for both species.  相似文献   

17.
The effects of high‐carbohydrate diets on endocrine status and gene expression of hepatic metabolism enzymes in genetically improved farmed tilapia (GIFT) juveniles (Oreochromis niloticus) at different sampling times were investigated. Two isonitrogenous (28% crude protein) and isolipidic (5% crude lipid) diets were formulated to contain 40% gelatinized wheat starch (WS) or glucose (GLU). Diets were assigned to triplicate groups of 30 fish (initial weight: 51 g) for 42 days. At the end of the trial, there were no significant difference in growth between WS group and GLU group. Hepatosomatic and visceral index and liver glycogen in juveniles were not significantly influenced by high‐carbohydrate diets on Day 42 (> 0.05). Plasma glucose was higher in the GLU group than the WS group from Day 7 onwards. Data on gene expression showed that G6PD, but not GK and G6Pase, were affected by high‐carbohydrate diet. The levels of G6PD in the GLU group on Days 35 and 42 were significantly higher than those in the WS group. Serum IGF‐I levels of both groups significantly decreased on Day 42, whereas serum insulin levels were unaltered by high‐carbohydrate diet. The serum GH level decreased significantly in the GLU group compared with the WS group. Overall, our data suggest that high‐glucose diets were efficiently used as an energy source by GIFT tilapia juveniles, and significantly affected liver lipogenic activity and serum GH levels.  相似文献   

18.
Five isonitrogenous and isoenergetic diets with soybean oil (SO), linseed oil (LO), algae oil from Schizochytrium sp. (AO), mixed oil (MO, SO:LO:AO = 1:1:1) and fish oil (FO; control group) were selected to feed juvenile Onychostoma macrolepis (initial weight 1.86 ± 0.07 g) for eight weeks. The results showed that in the LO and FO groups fish grew best. There was no significant difference in specific growth rate (SGR) and feed efficiency (FE) between the MO and FO groups (p > .05). The highest contents of 18:2n‐6, 18:3n‐3 and 22:6n‐3 in liver and muscle were found with the SO, LO and AO groups, respectively (p < .05). There appeared the highest malondialdehyde (MDA) content and the lowest superoxide dismutase (SOD) activity in the fish liver of the AO group. There appeared the highest concentrations of serum glucose (GLU), cholesterol (CHOL) and triglycerides (TG) in the SO group. The expressions of lipid anabolism genes were significantly up‐regulated by dietary SO and LO (p < .05). The expressions of lipid catabolism genes were significantly higher with the AO, MO and FO groups (p < .05). This study recommended that LO or MO as a better vegetable oil source for juvenile O. macrolepis.  相似文献   

19.
The feeding trial was conducted to evaluate the potentials of Clostridium butyricum in the diet of tilapia. Fish (~14 g) were fed with basal diet supplemented with 0 (Control), 0.5 (C‐1), 1 (C‐2), 2 (C‐3), 4 (C‐4) and 8 (C‐5) g/kg commercial probiotic‐containing C. butyricum (1.5 × 108 CFU/g) for 8 weeks. The results showed that weight gain significantly increased, and feed conversion ratio decreased in the C‐2, C‐3 and C‐4 groups (p < .05). The protein retention (except C‐1 group), lipid retention and apparent digestibility coefficient (ADC) of dry matter in probiotic supplementation groups were significantly enhanced, and ADC of protein in the C‐4 group was also improved (p < .05). The supplementation of probiotic significantly increased villus height in anterior intestines and reduced the numbers of intestinal Escherichia coli (p < .05). High‐throughput sequencing showed that top three phyla namely Planctomycetes in all probiotic‐containing groups, Proteobacteria in the C‐1 and C‐2 groups and Chloroflexi in the C‐3 group had higher level than the NC group. The cumulative mortality was reduced by dietary probiotic after challenging with Aeromonas hydrophila (p < .05). In conclusion, C. butyricum can be supplemented at 1–2 g/kg feed for promoting the growth, feed utilization, gut health and microbiota of tilapia.  相似文献   

20.
Three groups of juvenile golden pompano, Trachinotus ovatus (54.75 ± 0.25 g), were each fed one of three diets containing different lipid sources: fish oil (FO), soybean oil (SO) and lard oil (LO). Fish were reared in sea cages for 8 weeks, and the fish fed the FO diet had significantly higher specific growth rate (SGR) but lower condition factor (CF) than the other treatments. The fatty acid (FA) composition of whole‐body lipids was closely correlated with those in the diets. Although no differences can be found in hepatic fatty acid synthase (fasn) activity, the carnitine palmitoyl transferase 1 (cpt1) activity in fish fed the FO diet was significantly higher compared with other treatments. In addition, the relative gene expression of lipid metabolism‐related enzymes, such as cpt1, fas, apolipoprotein B100 (apoB100), delta‐6 fatty acyl desaturase (fadsd6) and fatty acid‐binding protein 1 (fabp1), was also influenced by the different dietary lipid sources. Serum triglyceride (TG) and glucose content in fish fed the LO and FO diets were significantly higher than those in the SO group. Accordingly, it can be concluded that FO could not be completely replaced by SO or LO in golden pompano diets. The lipid sources of a diet could impose significant influence on body condition factor and hepatic lipid metabolism of golden pompano.  相似文献   

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