质粒pUC19-CM-D的构建及应用

[目的]用克隆载体pUC19构建乳杆菌自杀质粒及乳杆菌基因缺失工程菌[方法]在质粒pUC19的多克隆位点插入氯霉素抗性基因构建pUC19-CM载体;在pUC19-CM载体氯霉素抗性基因的两侧均添加1个用于同源重组的同源臂,再构建成自杀质粒pUC19-CM-D。将自杀质粒pUC19-CM-D转化乳杆菌进行抗性筛选即可得到目标基因被抗性基因替换的突变株。[结论]pUC19-CM-D质粒的构建及应用为乳杆菌基因缺失工程菌的构建提供了一个快速有效的手段,也为乳杆菌基因功能研究奠定了基础。     

Research on the Construction of Bacterial Artificial Chromosome Vector DNA and the Potentials in Application

[Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119-Bluelox BAC.[Method] With selecting a proper single restriction site,sequences of a single copy BAC vector plasmid were inserted into proper site of High-copy plasmid pUC119 vector.[Result] The gene sequence of BAC vector lost control function of single copy number in new plasmid pUC119-BAC and was copied through High-copy form. The gene sequence of BAC vector basic function was completely cutted off through single enzyme digestion and the control function of single copy could be recovered by auto-connection.[Conclusion] The High-copy pUC119-BAC plasmid was used to copy and amplify high copy of basic function gene sequence in BAC vector,besides that it could be used to construct transfer vector of molecular cloned recombinant virus or BAC library.     

一种用高拷贝质粒载体制备BAC载体基本功能基因的新方法(英文)

[Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119-Bluelox BAC.[Method] With selecting a proper single restriction site,sequences of a single copy BAC vector plasmid were inserted into proper site of High-copy plasmid pUC119 vector.[Result] The gene sequence of BAC vector lost control function of single copy number in new plasmid pUC119-BAC and was copied through High-copy form. The gene sequence of BAC vector basic function was completely cutted off through single enzyme digestion and the control function of single copy could be recovered by auto-connection.[Conclusion] The High-copy pUC119-BAC plasmid was used to copy and amplify high copy of basic function gene sequence in BAC vector,besides that it could be used to construct transfer vector of molecular cloned recombinant virus or BAC library.     

PttGA20-氧化酶基因dsRNA抑止载体的构建(英文)

[Objective] Genetic Engineering technology was used to regulate the expression of PttGA20-oxidase gene thus restrained plant height growth and internode elongation for cultivating dwarfed plant.[Method] Based on the RNAi principle,the gene specific sequences of PttGA20-oxidase in the antisense and sense orientations interrupted by a gene sequence from GUS were cloned into a binary vector pBI121.The selection marker gene npt Ⅱ was replaced with bar gene to RNAi plasmid.[Result] After undergone different endonuclease restrictions,the constructed constraint vector released different segments whose sizes were similar to that of target segment,which demonstrated that the RNAi plasmid of PttGA20-oxidase gene was successfully constructed.[Conclusion] The experiment provided a new way for culturing dwarfed plant.     

转基因大豆MON89788检测质粒标准分子构建与应用

[目的]构建适用于转基因大豆MON89788检测的质粒标准分子.[方法]利用定性PCR和连接、转化等分子克隆技术,将大豆内标准基因lectin、MON89788的3'端特异性序列和5'端特异性序列依次克隆到pMD18-T载体上,获得质粒标准分子pMD-LM3M5,并进行适用性验证.[结果]获得了3 700 bp的质粒标准分子,其中重组DNA片段1 029 bp.该质粒标准分子的定性PCR检测灵敏度达到10 copy.[结论]该研究构建的质粒标准分子pMD-LM3M5能替代NON89788基体标准品,用于MON89788大豆及其产品的定性PCR检测. Abstract： [Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788.[Method]the lectin gene sequence,3'-junction and 5'-junction sequence between host plant DNA integrated DNA of MON89788 soybean were amplified independently,and the three fragments were cloned into the cloning vector pMD18-T in order through molecular manipulation method to construct pMD-LM3M5,the applicability of the constructed novel PRM was tested. [Result] Sequencing confirmation result showed that the PRM was 3 700 bp in length,containing 1 029 bp of recombined DNA fragment.The limits of qualitative detection of the PRM were 10 copies,[Conclusion]The PRM constructed in this study was suitable for the identification of MON89788 event.     

PttGA20-氧化酶基因dsRNA抑止载体的构建(英文)

[Objective] Genetic Engineering technology was used to regulate the expression of PttGA20-oxidase gene thus restrained plant height growth and internode elongation for cultivating dwarfed plant.[Method] Based on the RNAi principle,the gene specific sequences of PttGA20-oxidase in the antisense and sense orientations interrupted by a gene sequence from GUS were cloned into a binary vector pBI121.The selection marker gene npt Ⅱ was replaced with bar gene to RNAi plasmid.[Result] After undergone different endonuclease restrictions,the constructed constraint vector released different segments whose sizes were similar to that of target segment,which demonstrated that the RNAi plasmid of PttGA20-oxidase gene was successfully constructed.[Conclusion] The experiment provided a new way for culturing dwarfed plant.     

RNAi Plasmid Construction of PttGA 20-oxidase Gene

[Objective] Genetic Engineering technology was used to regulate the expression of PttGA20-oxidase gene thus restrained plant height growth and internode elongation for cultivating dwarfed plant.[Method] Based on the RNAi principle,the gene specific sequences of PttGA20-oxidase in the antisense and sense orientations interrupted by a gene sequence from GUS were cloned into a binary vector pBI121.The selection marker gene npt Ⅱ was replaced with bar gene to RNAi plasmid.[Result] After undergone different endonuclease restrictions,the constructed constraint vector released different segments whose sizes were similar to that of target segment,which demonstrated that the RNAi plasmid of PttGA20-oxidase gene was successfully constructed.[Conclusion] The experiment provided a new way for culturing dwarfed plant.     

N-乙酰胞壁质酶缺失对保加利亚乳杆菌LJJ自溶及形态的影响

【目的】乳酸菌自溶在发酵乳制品生产中广泛存在,自溶的速率和程度可以对产品的质量、风味、生产周期产生重要影响,在整个发酵过程中非常重要。N-乙酰胞壁质酶作为肽聚糖水解酶中的重要组成,可以破坏细胞壁完整性,在乳酸菌自溶过程中发挥着重要作用。论文旨在研究N-乙酰胞壁质酶缺失对保加利亚乳杆菌自溶的影响及对其形态的影响。【方法】分别以保加利亚乳杆菌基因组和pMG36e载体为参考序列设计引物,PCR分别扩增保加利亚乳杆菌中N-乙酰胞壁质酶的上下游同源臂基因m-up, m-down和pMG36e中的红霉素抗性基因。上述基因经过测序验证后,将经Kpn Ι,Xba Ι双酶切的红霉素抗性基因和pUC19相连接形成重组载体pUC:Emr并进行验证。随后,将经Kpn Ι,Sac Ι双酶切的m-down和重组载体pUC:Emr相连接形成重组载体pUC:Emr:m-down并进行验证。最后,将经Pst Ι,Xba Ι双酶切的 m-up与重组载体pUC:Emr:m-down相连接形成重组载体pUC:m-up:Emr:m-down并进行验证。以重组载体pUC:m-up:Emr:m-down作为N-乙酰胞壁质酶的同源重组敲除组件, 在电转化条件电压1.5 kV,电阻400 Ω和电容25 μF下,电转入保加利亚乳杆菌,在含有红霉素的MRS琼脂平板上筛选N-乙酰胞壁质酶基因敲除突变菌株并进行PCR验证。采用核酸溶出法检测基因缺失菌株与野生型菌株间的自溶度变化。扫描电子显微镜观察基因缺失菌株与野生型菌株间的形态变化。【结果】构建得到在N-乙酰胞壁质酶中间插入红霉素抗性基因为筛选标记的敲除组件用于保加利亚乳杆菌中N-乙酰胞壁质酶的基因敲除。获得带有红霉素抗性基因的N-乙酰胞壁质酶缺失的保加利亚乳杆菌突变菌株。突变菌株相比野生型菌株自溶特性及形态均发生显著变化,其中自溶度显著降低,37℃下自溶24 h,野生型菌株的自溶度约为73%,而突变菌株的自溶度约为35%,自溶度降为原来的1/2。野生型菌株的单个菌体细胞长度约10 μm,突变菌株的单个菌体细胞约30-40 μm,相比野生型菌株约增长了3-4倍。【结论】N-乙酰胞壁质酶在保加利亚乳杆菌自溶与细胞分裂过程中起着重要作用。保加利亚乳杆菌中N-乙酰胞壁质酶的缺失会导致菌体自溶的降低和菌体增殖过程中的细胞分裂受阻,产生菌体长度增长约3-4倍的菌体细胞。     

牛瑟氏泰勒虫P23主要表面蛋白基因的克隆及原核表达

[目的]研究牛瑟氏泰勒虫P23表面蛋白基因的克隆及原核表达.[方法]采用PCR方法扩增牛瑟氏泰勒虫中国延边株P23基因片段,将扩增产物克隆人pMD18-T载体构建重组质粒pMD18-P23经PCR、双酶切鉴定后测序;将目的基因片段亚克隆人表达载体pGEX-4T-1构建重组表达质粒pGEX-4T-P23,转化宿主菌BL21获得重组菌.通过对诱导条件的优化,根据SDS-PAGE确定表达蛋白的最佳表达条件;Western-blotting检测表达蛋白的反应原性.[结果]所克隆的牛瑟氏泰勒虫P23基因片段长507 bp,与牛瑟氏泰勒虫日本株P23基因的核苷酸同源性达99.4%,表达的融合蛋白大小约为46 ku;诱导时机以接种培养后2 h为最佳,诱导时间以6 h为最佳,诱导温度以34℃为最佳,0.008～1.000 mmol/L的IPTC对表达量的影响不大.Western blotting检测表明该蛋白具有较好的抗原性.[结论]为牛瑟氏泰勒虫病的免疫学诊断和预防等研究奠定了基础. Abstract： [Objective] The aim was to study cloning and prokaryotic expression of P23 major surface protein gene of Theileria sergenti.[Method]A pair of specific primers was designed according to the sequence of P23 major surface protein of T.sergenti (D84447).The P23 gene was amplified by PCR from genomic DNA of T.sergenti and cloned into pMD18-T vector to construct recombinant clonal vector pMD18-P23.Positive clones were identified by PCR screening and restriction digestion.A recombinant expression plasmid pGEX-4T-P23 was constructed by subcloning the cloned P23 gene into the linearized pGEX-4T-1 vector and transformed into E.coil BL21.After introduction by IPTG,the expressed fusion protein was identified by SDS-PAGE and Western-blotting. [Result] The cloned gene has a total length of 507 bp.Sequencing result showed that the nucleotide sequence of the cloned P23 gene shared 99.4% identity with that of P23 published in GenBank (D64447).The expressed fusion protein was 46 ku in molecular mass.Induction opportunity of zhours after culture inoculation was the best,the induction time of 6 h was the best,and induction temperature of 34 ℃ was the best as well,IPTG of 1 mmol/L had little effect on the expression.Western-blotting indicated that recombinant protein was recognized by specific antibody. [Conclusion] This study would lay a foundation for further research on the prevention and diagnose of T,sergenti.     

金黄色葡萄球菌FnBP配体结合区基因的克隆及其原核表达(英文)

[Objective] The study aimed to clone the FnBP ligand binding gene of Staphylococcus aureus and run prokaryotic expression by constructing a prokaryotic expression vector. [Method] The gene encoding FnBP ligand binding gene was amplified from S.aureus chromosomal DNA by PCR technique. After T-A cloning, plasmid pMD18- FnBP was constructed. pMD18- FnBP and pET28a(+)were digested by BamH Ⅰ and EcoR Ⅰ double enzymes, then the purified FnBP ligand binding gene was subcloned into the expression vector pET28a(+), and the prokaryotic expression vector pET28a-FnBP was thus constructed. The constructed plasmid pET28a-FnBP was transformed into Escherichia coli BL21(DE3) competent cells. The bacterium was induced by IPTG and the expressed products were analyzed by SDS-PAGE and Western blot. [Result] The gene fragment with the length of 370 bp was amplified by PCR approach. One approximately 30 kD exogenous protein was observed in SDS-PAGE analysis. Western blot analysis indicates the protein has antigenicity of S.aureus. [Conclusion] The FnBP ligand binding gene of S.aureus was successfully cloned and expressed in prokaryotic cells.     

牛体外发育胚胎特定阶段差异表达基因的研究

[目的]研究不同发育时期牛体外受精胚胎在基因表达模式上的差异.[方法]利用单个胚胎mRNA差异显示技术,对单个8细胞期胚胎与囊胚进行mRNA差异显示,获得1条特异表达条带,对其进行克隆、测序,并与GenBank进行对比.[结果]该序列与牛核糖体蛋白131基因(ribosomal protein 131,RPL3])具有99%的同源性.采用实时定量PCR技术检测8细胞期和囊胚期胚胎RPL31的mRNA表达量,结果表明,RPL31在8细胞期胚胎的相对表达量为囊胚期胚胎的3.2倍.[结论]为揭示和阐明控制牛早期胚胎发育的相关机理提供依据. Abstract： The cattle different stage embryos obtained from in vitro was studied using the technology of single preimplantation embryo mRNA different display:single 8-cell and blastocyst stage embryos were studied using technology of mRNA different display and one different fragment was found.The result suggested that this fragment displayed high homology (99%) to cattle mRNA for ribosomal protein L31.Then to detect the expression of RPL31 mRNA in 8 cell and blastocyst stage embryos by real-time quantitative PCR,the result showed the relative amount of 8 cells was 3.2 times of blastocyst's.     

用最短路径传递共表达预测拟南芥基因功能

提出基因之间传递共表达可作为一个重要因素来连接同一代谢通路中的基因,而同一代谢通路中的功能相似的基因都是高表达相关的.因此可通过求代谢通路下的最短路径,南同在一条最短路径上的与未知基因高表达相关的已知基因来预测末知基因的功能.通过用最短路径算法分析拟南芥代谢通路下的共表达数据对未知基因的功能进行预测,证明了此方法可以预测出拟南芥代谢通路下未知基因的功能,并验证了通过在代谢通路下求最短路径来预测基因功能的方法具有一定的可行性和有效性. Abstract： The present paper predicted the function of unknow genes by analyzing the co-expression data of Arabidopsis thaliana from biological pathway based on the shortest-path algorithm.This paper proposed that transitive co-expression among genes can be used as an important attribute to link genes of the same biological pathway.The genes from the same biological pathway with similar functions are strongly correlated in expression.Moreover,the function of unknown genes can be predicted by the known genes where they are strongly correlated in expression lying on the same shortest-path from the biological pathway.Analyzing the Arabidopsis thaliana from the biological pathway,this study showed that this method can reliably reveal function of the unknown Arabidopsis thaliana genes and the approach of predicting gene function by transitiving coexpression in shortest-path is feasible and effective.     

底栖鱼类对水田上覆水中磷素动态的扰动效应

[目的]研究底栖鱼类泥鳅对水田上覆水中磷素动态的扰动效应,探讨生物扰动机制.[方法]基于模拟试验,使用离子色谱法和分光光度法,对比分析上覆水中磷素含量在有/无泥鳅活动时的差异.[结果]扰动组的TP、DTP和PP浓度在试验开始阶段与对照组无显著差异,在试验中、后期显著高于对照(P<0.05).扰动组要的PP/TP高于对照组,扰动组中TP浓度的增加主要是由于PP的增加,扰动组的DIP/DT在试验中、后期显著高于对照(P<0.05).[结论]底栖鱼类对水田上覆水中的磷素产生了扰动作用,增加了水稻生长可利用的的磷素养分. Abstract： [Objective] The research aimed to investigate the bioturbation effects of benthic fish Misgurnus anguillicaudatus on phosphorus dynamic in overlying water of paddy field,as well as to explore the bioturbation mechanism.[Method]Based on simulation experiment,the phosphorus contents in overlying water were analyzed comparatively with and without Misgurnus anguillicaudatus by the using of ion chromatography and spectrophotometry. [Result] The concentrations of total phosphorus (TP),dissolved total phosphorus(DTP)and particular phosphorus(PP) in bioturbation group had no significant differences with those in control group in initial stage of experiment,and became significantly higher than control group in middle and late stages of experiment(P<0.05).The PP/TP ratios in bioturbation group were bigger than those in control group,the increase of TP concentration in bioturbation group was mainly due to the increase of PP.The ratios of dissolved inorganic phosphorus(DIP) to DTP (DIP/DTP) were significantly bigger than those in control group in middle and late stages of experiment (P<0.05). [Conclusion] The benthic fish had bioturbation effects on phosphorus in overlying water of paddy field,which increased the available phosphorus for rice growth.     

抗黄瓜花叶病毒RNAi载体的构建及烟草的转化

[目的]构建抗黄瓜花叶病毒RNAi载体,并将载体转入烟草.[方法]采用RT-PCR方法,扩增黄瓜花叶病毒NS04加工番茄分离物的RNA2基因组的序列选取CMV RAN2基因组中的复制酶片段作为靶序列,构建pBi35SCR2真核表达载体,并对表达载体时行鉴定;通过农杆菌介导的方法将表达载体转入烟草,用PCR的方法检测载体是否转入.[结果]系统进化树分析结果表明,RNA2中编码CMV-2a的序列与中国浙江的DQ412731 分离物有较高核苷本乡酸及氨基酸同源性,分别达到98.0%和96.5%;RCR结果表明,试验成功构建了pBi35SCR2真核表达载体,并成功将表达载体转入烟草[结论]试验获得的转基因烟草可作为后期攻毒试验的材料,并为研究加工番茄抗黄瓜花叶病毒奠定了基础. Abstract： [Objective] Aimed to construct RNAi vector resistant to cucumber mosaic virus and transferred this vector into tobacco.[Method]RT-PCR method was used to amplify cucumber mosaic virus NSO4 and process RNA2 gene sequen of tomato isolates.The analysis results of phylogenetic tree demonstrated that the sequence in RNA2 encoded CMV-2a had 98.0% and 96.5% homology with nucleotide and amino acid of DQ412731 isolate of Zhejiang,China.The replicase fragment in CMV RAN2 gene was taken as target sequence to construct pBi35SCR2 eukaryotic expression vector,then the expression vector was identified.Through agrobacterium-mediated method,the expression vector was transferred into tabacco and PCR method was used to check the transfer.The PCR results demonstrated that the experiment had successfully construct eukaryotic expression vector of pBi35SCR2 and the expression vector was successfully transferred into tabacco. [Conclusion] The obtained transgenic tobacco could be used as challenge test material in following experiment and provided foundation for studying processing tomato resist cucumber mosaic virus.     

一株产紫杉醇的曼地亚红豆杉内生真菌的分离及鉴定

[目的]分离并鉴定1株产紫杉醇的曼地亚红豆杉内的内生真菌.[方法]从曼地亚红豆杉树皮内表皮中分离得到32株内生真菌,并通过高效液相色谱法检测其发酵产物.[结果]筛选获得1株可以产紫杉醇的内生真菌M57,其紫杉醇产量为45～50μg/L,并通过对M57菌落的形态学观察以及18S rDNA序列分析初步将其鉴定为根霉属(Rhizopus)真菌.[结论]该菌株的发现为微生物发酵法生产紫杉醇提供了具有潜在应用价值新的菌种. Abstract： [Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media.[Method]32 strains of endophytic fungi were identified form the inner bark of T.media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp.through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp.M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.     

基于蒙特卡洛多重积分的DNA动力学结构参数的计算

依据最新NDB数据库中蛋白质-DNA复合物晶体结构数据,基于修正的DNA结构统计力学模型,利用蒙特卡洛多重积分计算DNA动力学结构的有关参数,并对计算得到的结果进行时间复杂度和精确度分析. Abstract： Based on protein-DNA complex crystal structural data in up-to-date Nucleic Acid Database,the related parameters of DNA Kinetic Structure were investigated by Monte-Carlo Multiple Integrals on the base of modified DNA structure statistical mechanical model,and time complexity and precision were analyzed on the calculated results.     

电场作用下人工湿地植物的生理生化响应

通过测定人工湿地植物叶片中叶绿素含量、MDA含量及SOD酶活性.研究不同通电强度下人工湿地植物生理特性变化,分析电场对植物生理特性的影响机理,为利用电场强化人工湿地去除污染物提供依据.研究结果表明,与对照植物相比,1和3 V低强度电压对植物正常生理指标变化无明显影响,且生长趋势优于对照组;随着电压强度的升高,植物叶片中叶绿素含量、MDA含量以及SOD酶活性受到较大影响,表明植物体受到较强的氧化胁迫,生长受到危害.合适的电场能够强化人工湿地的污水处理效果. Abstract： By measuring wetland plants chlorophyll content,malondialdehyde (MDA) content and superoxide dismutase (SOD) enzyme activity,the changes of wetland plant physiological characteristics under different power strength were studied,and the mechanism of electric field on plant physiological characteristics was analyzed to provide a theoretical basis for the pollutant removal ability strengthening of artificial wetland under electric field.The results showed that compared with the control plants,low-intensity-voltage (1 V and 3 V) had no significant effect on the normal physiological and biochemical indexes of the plants,and the growth trend was better than the control group;with the voltage increasing,plant chlorophyll content,MDA content and SOD activity were greatly affected,indicating that plants were under strong oxidative stress,and the growth was damaged.Therefore,a suitable electric field could enhance the sewage treatment effect of constructed wetland.     

催产素在广西本地水牛下丘脑-垂体-卵巢轴的免疫组化定位

[目的]研究催产素(OT)在广西本当水牛下丘脑、垂体及卵巢的分布上的联系,进而了解OT在下丘脑、垂体及卵巢3者之间分泌释放途径.[方法]采用免疫组织化学SuperPicTureTM二步法检测5头广西本地水牛的下丘脑、垂体和卵巢中催产素(OT)的分布情况.[结果]下丘脑中分泌OT的神经元主要分布在弓状核、视上核及室旁核,在腹内侧核、腹外侧核、交叉上核、背内侧核、乳头体、下丘脑前核等核团也有一定数量的阳性神经元;在腺垂体发现OT免疫反应阳性产物,自垂体柄和正中隆起的一侧可见到平行排列的OT阳性神经纤维断续地延伸至神经部;卵巢中只在生殖上皮、卵泡颗粒细胞和黄体细胞发现有大量OT免疫阳性产物.[结论]首次发现OT存在于广西本地水牛下丘脑-垂体-卵巢轴的各个环节,并且下丘脑中各主要核团均有OT免疫阳性神经元的分布,尤其以弓状核、视上核、室旁核分布最多,为进一步研究OT的合成和作用机制提供形态学依据,并对广西本地水牛的繁殖育种及泌乳起到一定的参考指导作用. Abstract： To study the association of oxytocin (OT)'s distribution in hypothalamatic,pituitary and ovary,and understand how the OT secrete releasing in hypothalamus,pituitary and ovaries,the paraffin section immunohistochemistry SuparPicTureTM two step method was used to detect the distribution of OT in hypothalamatic-pituitary-ovary axis of five femal Guangxi local buffalo.The test results could provide morphology according to study the OT's synthesis and mechanism of action ,and could play reference and directions part in breeding Guangxi local buffalo.The test results display:oxytocin immuno reactive (OT-IR) neuronsw eremainly distributed arcuate nucleus,supraoptic nucleus and paraventricular nucleus,and OT-IR neurons was also found in ventrornedial nucleus,ventrolateralis nucleus,suprachiasrnaticus nucleus,dorsomediai nucleus,mamillary body,anterior hypothalamic nucleus and so on.The OT immunoactive production was found in pituitary and few OT-IR nerve fibers extended to post pituitary from hypophyseal stalk and medium eminence.In ovaries,OT immunoactive productions were only distributed in germinal epithelium cells,granulosa cells and lutein ceils.The OT was first discovered in singulorum link of hypothalamatic-pituitary-ovary axis of Guangxi local buffalo.The OT immunoactive neurons were first discovered in every main nucleus of Guangxi local buffalo hypothalamus,especially distributed in arcuate nucleus,supraoptic nucleus and paraventricular nucleus.     

干旱胁迫对苗期甘蔗叶片水分和叶绿素荧光参数的影响

[目的]研究干旱胁迫对甘蔗叶片水分和叶绿素荧光参数的影响,为甘蔗生产及评价研究提供依据.[方法]选取7个抗旱性不同的甘蔗品种,在苗期进行干旱胁迫,并测定胁迫条件下甘蔗叶片水分含量和叶绿素荧光参数的变化.[结果]甘蔗叶片水势和相对含水量与土壤相对含水量存在一定的内在联系,耐旱强的品种对土壤水分的利用率较高;相关分析和因子分析表明茁期干旱存活率、Fc/Fm、叶片水势和相对含水量可被用作抗旱性评价指标.[结论]水势表现为一个相对独立的影响因子,对甘蔗抗早性有支配作用,并验证了Fv/Fm作为甘蔗抗旱评价指标的可靠性. Abstract： [Objective] The aim was to explore the effects of water stress on leaf water and chlorophyll fluorescence parameters of sugarcane seedling,as well as to provide basis for the study on sugarcane production and evaluation.[Method]Seven different sugarcane varieties were studied at the seedling stage under drought stress,and the changes of leaf water and chlorophyll fluorescence parameters under stress conditions were detected. [Result] leaf water potential,leaf relative water content and soil relative water content showed a certain amount of internal relationship,the sugarcane varieties that had more tolerant to drought had higher utilization rate of soil water;the correlation analysis and factor analysis suggested that the survival rate at seedling stage under drought stress,Fv/Fm,leaf water potential and relative water content could be used as drought resistance evaluation indicators. [Conclusion] As a relatively independent influencing factor,water potential had dominating effect on drought resistance,and the reliability of Fv/Fm as drought resistance evaluation indicator had been verified.     

中草药提取液对黄瓜苗期杀根结线虫的活性研究

[目的]研究中草药提取液对黄瓜苗期杀根结线虫的活性.[方法]利用植物水培技术,结合生长条件易控制、根系生长过程易连续观察的特点,应用5种具有较强室内触杀根结线虫活性的植物提取液及其与阿维菌素的复配液,对黄瓜苗期水培根系杀线活性进行系统研究.[结果]胡黄连和石榴皮提取液可以在不影响水培黄瓜苗期植株生长的前提下表现出对根结线虫良好的活体植株根系寄生防治和杀灭活性,且达到了与阿维菌素相近的作用水平;而狗脊、木香和蛇床子提取液对黄瓜的生长表现出不同程度的抑制作用,其对根结线虫的防治效用和杀灭活性也较弱.[结论]触杀效果和杀线活性成分能否被植物吸收利用共同制约着具有杀线活性中草药提取液的开发应用. Abstract： Present mature plants hydroponic technology was used,combined with some excellent characteristics,such as growth conditions was easy to control and process of root growth was easy to continuously observe,the nematicidal activity of 5 kinds of Chinese herbs extracts and the compound solution of Avermectin,with strong contact toxicity effect indoor,was systematically studied and investigated the affection on the rootknot nematode parasitized on the cucumber seeding stage.It is found that under the premise of no influence on root growth of cucumber,extracts from Picrorhiza scrophulariiflora and Punica granatum showed strong prevention and nematicidal activity,and had the similar efficacy of Avermectin;while the extracts from Cibotium barornetz,Aucklandia lappa Decne and Fructus cnidii showed low nematicidal activity and various degrees inhibition effect on plant growth.