向日葵抗列当水平室内鉴定体系准确性评价及不同品种抗列当水平鉴定

向日葵列当是一种寄生在向日葵根部的寄生性种子植物。向日葵被列当寄生后表现为植株矮化、花盘减小、产量急剧下降,含油量和蛋白含量也明显降低,严重时可导致向日葵绝收。利用12份在田间条件下鉴定出抗性水平的向日葵品种对室内建立的列当寄生体系(培养皿滤纸体系)鉴定结果的准确性进行了评价。在此基础上对供试的80份不同向日葵品种的抗列当水平进行了室内鉴定。结果表明,供试的47份食葵品种中以TP3313为代表的3份品种表现为免疫水平,所占比例为6%;而以JK601为代表的11份品种呈现高抗水平,占比为24%;其余品种均呈现感病和高感水平。供试的33份油葵品种中以F917为代表的11份品种表现为免疫水平,所占比例为33%;而以赤CY102为代表的14份品种呈现高抗水平,占比为43%;感病和高感水平品种占比为3%。整体看来,油葵品种对列当的抗性水平显著高于食葵品种。     

向日葵抗列当品种(系)筛选及其应用评价

为了全面评价不同向日葵品种(系)对列当的抗寄生性和其在生产中的应用价值,对‘TP3313’等50个食葵品种(系)、‘内蒙杂4号’等6个油葵品种(系)进行了田间自然寄生试验,结果表明:食葵‘TP3313’为对列当免疫的品种,且病虫害发生较轻,产量相对较高(261.11kg/667m~2),因而具有极高的推广应用价值;‘TP3314’为高抗列当品种,但是受向日葵菌核病危害较重,产量为219.64kg/667m~2;‘SH361-L’、‘SH338-M’等8个品种表现为中感,属耐列当品种(系),并且产量相对较高,在生产中具有一定的应用价值。     

向日葵新品种抗列当水平的田间鉴定

向日葵列当是一种寄生性杂草,严重危害向日葵产业的发展。为有效鉴定向日葵登记品种列当抗性、加快优良品种推广应用,本研究选取国内具有代表性的36个食用向日葵登记品种,在内蒙古向日葵主产区乌拉特前旗芦管壕村和四子王旗高油房村进行了品种列当抗性田间鉴定。结果表明,乌拉特前旗芦管壕村试验点列当生理小种为F小种,‘同欣2号’‘诚牌C93’等20份品种在该点表现为免疫水平;‘三瑞3号’‘天葵16’等11份品种呈现出高抗水平;其余5份品种田间表现为易感或者高感水平。四子王旗高油房村试验点列当生理小种为G小种,‘同欣2号’‘圣地壹号’等14份品种在该点表现为免疫;‘诚牌C93’‘三瑞3号’等16份品种表现为高抗;‘龙食葵5号’表现为中抗;其余5份品种表现为易感或者高感。其中,在两个地区均表现为免疫的品种共有13份,分别是‘同欣2号’‘圣地壹号’‘龙葵27’‘圣地777’‘启源6号’‘JR5511’‘益民931’‘益民303’‘益民966’‘益民969’‘金谷葵33’‘S2009’‘新农101’;呈现高抗列当的品种有9份,分别是‘三瑞3号’‘天葵16’‘正博鼎盛’‘双星6号’‘中食葵90’‘益民968’‘...     

阴山北麓地区向日葵列当生理小种的鉴定及种子的单株纯化

向日葵列当是一种全寄生的种子植物,对向日葵生产造成严重的影响,而选育抗列当品种是目前防控向日葵列当最为有效的防控措施。由于向日葵列当种群存在生理小种分化现象,因此明确不同地区的向日葵列当生理小种的类型以及分布对于抗列当品种的布局非常重要。本研究利用国际通用的向日葵列当生理小种鉴别寄主,对阴山北麓地区的武川县和四子王旗共计7个向日葵地块中采集的列当种子的生理小种进行了鉴定,结果表明除了呼和浩特市武川县头号村采集的向日葵列当样本为E小种外,其他6个不同地点采集到的向日葵列当样本均鉴定为G小种。为了更好地对向日葵品种抗列当水平进行准确的评价,本研究选择了已经鉴定为E、F和G三个小种类型的向日葵列当的样本进行了单株纯化以及扩繁。扩繁得到的列当的种子将用于准确评价向日葵不同的育种材料和品种对列当不同生理小种的抗性水平。     

向日葵新品种对列当的抗性鉴定

通过田间自然寄生试验,鉴定了2个向日葵新品种对列当的抗性。结果表明,向日葵新品种TP3313列当寄生率为0%,抗寄生指数为1,为免疫品种,其他病虫害发生为害较轻,产量为261.1 kg/667m~2,具有较高的推广应用价值;TP3314向日葵列当的寄生率为0.4%,寄生指数为0.99,为高抗品种。但向日葵菌核病发生较重,产量为219.6 kg/667m~2,在生产应用过程中,应注意向日葵菌核病的防控。     

我国向日葵列当发生危害现状调查

向日葵列当是危害我国向日葵最严重的杂草。为明确我国向日葵田列当的发生危害情况及防治现状,采用对角线5点取样法对新疆、内蒙古、吉林和河北等主要向日葵种植区进行调查,对向日葵列当的寄生率和寄生强度等数据进行分析。结果表明,向日葵列当在向日葵产区广泛分布,严重危害向日葵的生产,其对食葵的寄生率高于油葵,对抗性品种的寄生率较低。在所有调查地区中内蒙古地区向日葵列当发生最严重,寄生率高达100%,寄生强度达到38.5,单株寄生数量最高,达118株;新疆地区的向日葵列当寄生率居中,吉林、河北向日葵列当发生相对较轻。通过实地调研结合文献报道,对目前国内外向日葵列当的研究进展进行了综述,研究结果为指导向日葵列当防治提供参考。     

向日葵筒状小花和瘦果性状与欧洲葵螟寄主选择的关系

为明确欧洲葵螟Homoeosoma nebulellum Denis et Schiffermüller寄主选择的物理机制,通过对14个向日葵品种（系）筒状小花及瘦果的形态指标观察,采用籽粒被害虫情指数进行田间寄主选择性试验,研究了向日葵的形态性状和欧洲葵螟寄主选择的关系。欧洲葵螟对不同向日葵品种（系）具有明显的选择性,且对食葵品种（系）的选择性均显著高于油葵品种（系）。食葵瘦果多为中长或长形（≥1.5 cm）,油葵瘦果则属于短或特短形（<1.5 cm）。向日葵各形态指标在不同品种（系）间均存在极显著差异,食葵的花盘直径、筒状小花开口、子房壁厚度、瘦果果壁厚度、瘦果果壁茸毛密度均大于油葵,且各指标与籽粒被害虫情指数之间、各指标互相之间也均呈显著正相关关系。表明选育较小花盘的向日葵品种（系）可减少葵螟对向日葵的为害。     

烟草及向日葵上列当Orobanche cumana的发生及其生物防治

 近年,在河北省部分烟草和向日葵种植地发现大量寄生性杂草列当发生并造成严重为害。经鉴定为向日葵列当Orobanche cumana Wallr.。向日葵、烟草、番茄和黄瓜等作物的根分泌物能够刺激向日葵列当O. cumana种子萌发,而油葵根的分泌物则不能刺激向日葵列当种子萌发。利用列当致病镰刀菌L2菌株进行田间生物防治,防治效果达到92.4%。L2菌株对小麦、玉米、棉花、烟草和向日葵等作物生长无影响,L2菌株粗毒素能够抑制列当种子萌发,并造成列当种子萌芽管损伤。     

向日葵列当综合防控技术集成与应用

向日葵列当是一种分布广、传播快、危害大、防治极为困难的全寄生性杂草,目前严重影响内蒙古乃至全国向日葵产业的健康发展。本研究在明确当地向日葵列当发生规律和列当优势小种的基础上,加强检疫;以种植抗性品种为主,采用清洁田园、适时播种、水肥调控、人工铲除和轮作等措施;以及施用诱抗剂和使用抗咪唑啉酮类除草剂向日葵品种的化学防治技术等,结合各地生产条件集成向日葵列当综合防治技术体系。2014—2018年该技术体系在内蒙古、新疆和黑龙江主要向日葵列当发生危害区推广应用21.30万hm2,田间防效达85%以上,挽回经济损失共计9.57亿元,有效遏制了向日葵列当的进一步危害,保障了我国向日葵产业的健康发展,取得了良好的经济、社会效益。     

不同向日葵品种对黄萎病的抗性*

采用浸根接种法鉴定不同向日葵种质资源对黄萎病的抗性差异。结果表明：40份供试向日葵品种依据抗性划分标准共分为3类,其中中抗品种有5份如：‘118’、‘新葵杂5号’等;低抗品种有8份如‘767’、‘KJ003’等;其余27份供试材料均呈现感病;没有鉴定出免疫和高抗的向日葵品种。     

Dermestid beetles in ‘evolution Canyon’, lower Nahal Oren, Mt. Carmel, including new records for Israel

Fifteen species of dermestid beetles were recorded at ‘Evolution Canyon’ (EC), Lower Nahal Oren, Mt. Carmel, Israel. They represent ~35% of known Israeli dermestid species. The following three species were recorded for the first time in Israel:Trogoderma svriaca Dalla Torre, 1911;Ctesias svriaca Ganglbauer, 1904; andAnthrenus (s.str.) jordaniens Pic, 1934. Adults of 13 species were collected on the more solar radiated, warmer and climatically more fluctuating south-facing slope (SFS); ten species were collected on the opposite, north-facing slope (NFS), which was cooler and climatically more stable. The abundance of adult dermestid beetles was 1.9 times higher on the SFS than on the NFS (86 and 47, respectively). Species richness and abundance distribution at EC (three collecting stations on each slope and one at the valley bottom) were significantly negatively correlated with the plant cover that consisted of trees and bushes (Spearmanr _s ,P=0.007 and 0.039, respectively) and perennials (Spearmanr _s ,P=0.039 and 0.077, respectively), indicating that non-woody plants were preferred by adult dermestid beetles.     

Epidemiology of Toxigenic Fungi and their Associated Mycotoxins for Some Mediterranean Crops

Recent data on the epidemiology of the common mycotoxigenic species of Fusarium, Alternaria, Aspergillus and Penicillium in infected or colonized plants, and in stored or processed plant products from the Mediterranean area are reviewed. Emphasis is placed on the toxigenicity of the causal fungal species and the natural occurrence of well known mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes, zearalenone, patulin, Alternaria-toxins and moniliformin), as well as some more recently described compounds (fusaproliferin, beauvericin) whose toxigenic potential is not yet well understood. Several Fusarium species reported from throughout the Mediterranean area are responsible of the formation of mycotoxins in infected plants and in plant products, including: Fusarium graminearum, F. culmorum, F. cerealis, F. avenaceum, F. sporotrichioides and F. poae, which produce deoxynivalenol, nivalenol, fusarenone, zearalenone, moniliformin, and T-2 toxin derivatives in wheat and other small grains affected by head blight or scab, and in maize affected by red ear rot. Moreover, strains of F. verticillioides, F. proliferatum, and F. subglutinans, that form fumonisins, beauvericin, fusaproliferin, and moniliformin, are commonly associated with maize affected by ear rot. Fumonisins, were also associated with Fusarium crown and root rot of asparagus and Fusarium endosepsis of figs, caused primarily by F. proliferatum. Toxigenic A. alternata strains and associated tenuazonic acid and alternariols were commonly found in black mould of tomato, black rot of olive and citrus, black point of small cereals, and black mould of several vegetables. Toxigenic strains of A. carbonarius and ochratoxin A were often found associated with black rot of grapes, whereas toxigenic strains of A. flavus and/or P. verrucosum, forming aflatoxins and ochratoxin A, respectively, were found in moulded plant products from small cereals, peanuts, figs, pea, oilseed rape, sunflower seeds, sesame seeds, pistachios, and almonds. Finally, toxigenic strains of P. expansum and patulin were frequently found in apple, pear and other fresh fruits affected by blue mould rot, as well as in derived juices and jams.     

A survey of the genera of<Emphasis Type="Italic">Microgaster</Emphasis> latreille and<Emphasis Type="Italic">Hygroplitis</Emphasis> thomson (Hymenoptera: Braconidae: Microgastrinae) from China

The genera ofMicrogaster Latreille 1804 andHygroplitis Thomson 1895 from China are presented systematically in this paper. Thirty-two species ofMicrogaster and three species ofHygroplitis are known in China. Diagnosis, character variation, distribution and host of each species among the two genera are presented, including its host and distribution. Keys to the species ofMicrogaster andHygroplitis are given. http://www.phytoparasitica.org posting Dec. 19, 2006.     

Plant Viruses Transmitted by Whiteflies

One-hundred and fourteen virus species are transmitted by whiteflies (family Aleyrodidae). Bemisia tabaci transmits 111 of these species while Trialeurodes vaporariorum and T. abutilonia transmit three species each. B. tabaci and T. vaporariorum are present in the European–Mediterranean region, though the former is restricted in its distribution. Of the whitefly-transmitted virus species, 90% belong to the Begomovirus genus, 6% to the Crinivirus genus and the remaining 4% are in the Closterovirus, Ipomovirus or Carlavirus genera. Other named, whitefly-transmitted viruses that have not yet been ranked as species are also documented. The names, abbreviations and synonyms of the whitefly-transmitted viruses are presented in tabulated form together with details of their whitefly vectors, natural hosts and distribution. Entries are also annotated with references. Whitefly-transmitted viruses affecting plants in the European–Mediterranean region have been highlighted in the text.     

Broad bean mottle virus in Morocco; curculionid vectors,and natural occurrence in food legumes other than faba bean (Vicia faba)

Broad bean mottle virus (BBMV) was transmitted from infected to healthy faba-bean plants by the curculionid weevilsApion radiolus Kirby,Hypera variabilis Herbst,Pachytychius strumarius Gyll,Smicronyx cyaneus Gyll, andSitona lineatus L. The latter appeared to be an efficient vector: acquisition and inoculation occurred at the first bite, the rate of transmission was c. 41%, and virus retention lasted for at least seven days.S. lineatus transmitted the virus from faba bean to lentil and pea, but not to the three genotypes of chickpea tested. This is the first report on the generaHypera, Pachytychius, andSmicronyx as virus vectors, and onA. radiolus, H. variabilis, P. strumarius, andS. cyaneus as vectors of BBMV.Out of 351 samples of food legumes with symptoms suggestive of virus infection, 16, 11, 19, and 17% of the samples of chickpea, lentil, pea, and common bean, respectively, were found infected when tested for BBMV in DAS-ELISA. This is the first report on the natural occurrence of BBMV in chickpea, lentil, pea, and common bean. The virus should be regarded as a food-legume virus rather than a faba-bean virus solely, and is considered an actual threat to food legume improvement programmes.     

Characterization of potato potyvirus Y (PVY) isolates from seed potato batches. Situation of the NTN, Wilga and Z isolates

A collection of 38 PVY isolates from seed potato batches, originating from several Western European countries, was characterized by using current biological, serological and molecular tools differentiating PVY strains and groups. The correlation between the three kinds of tests was good but not absolute. No single serological or PCR method was able to discriminate among the five isolate groups found. Twenty-nine isolates belonged to the PVY^N strain and six to the PVY^O strain. No PVY^C was found. Two other isolates reacted serologically like PVY^O, but were unable to elicit a hypersensitive response from the Ny_tbr gene and probably represent the PVY^Z group. At the molecular level, these two isolates showed a combination of both PVY^O and PVY^N and could be recombinants of these strains. Another isolate reacted serologically like PVY^O, but induced vein necrosis in tobacco, like PVY^N-Wilga. Some PVY^N isolates caused tuber ring necrosis in glasshouse conditions. These might belong to the PVY^NTN group. The PVY^NTN, PVY^N-Wilga and PVY^Z groups probably represent pathotypes within strains PVY^N and PVY^O, respectively. The present study also confirms previous reports showing a high genetic variation at the 5 end within the PVYN strain.     

The autumn leafroller: Phenology,damage and parasitoids in a Dutch apple orchard

The phenology of the autumn leafroller,Syndemis musculana, a local pest of apple, was studied in order to forecast larval emergence. From 1983–1986, peak flight as determined with sexpheromone traps was always between 13–18 May. The duration of embryonic development was determined at various constant temperatures and used to estimate the periods of egg hatch in these four years. Each year, most eggs should have hatched in the second decade of June.Differences in attack rates between apple cultivars seem to be explained largely by the variation in picking time. Larvae are only half grown at the beginning of harvest (cv. James Grieve), and have gone into hibernation when the latest variety (cv. Golden Delicious) is picked. Moreover, the varieties Cox's Orange Pippin and Belle de Boskoop, picked about half time, are liable to receive additional damage by caterpillars brought with the picked fruits into storage.Various hymenopterous parasites were reared from caterpillars. As the only leafroller in the orchard which hibernates as mature larva,S. musculana may promote winter survival of some parasitoids, like the eulophidColpyclypeus florus.Samenvatting De fenologie van de herfstbladroller (Syndemis musculana Hübner), een incidentele plaag op appel, werd nader bepaald met het doel het uitkomen van de eieren te kunnen voorspellen. In 1983–1986 viel de piekvlucht, bepaald met behulp van feromoonvallen, steeds tussen 13 en 18 mei.De ontwikkelingsduur van de eieren bij verschillende constante temperaturen werd gebruikt om de periode van uitkomen te schatten. De meeste eieren zullen ieder jaar in de eerste helft van juni uitkomen.Geconstateerde verschillen in schade tussen appelrassen blijken goeddeels terug te voeren op verschillen in pluktijdstip. De rupsen van de herfstbladroller zijn pas half-was als de eerste appels eind augustus geplukt worden, terwijl tegen het einde van de oogst begin oktober de meeste al in winterslaap zijn. Met name tussentijdse rassen, als Cox's Orange Pippin and Schone van Boskoop, lopen extra schade op doordat grotere rupsen met de geplukte vruchten in de kist terecht komen.Uit de rupsen werden negen, al van andere boomgaardbladrollers bekende, sluipwespen gekweekt, Omdat deze bladrollersoort, als enige in de boomgaard, als volgroeide rups overwintert, lijkt zij bij uitstek geschikt als winterwaard.This study was carried out at the Experimental Orchard De Schuilenburg, Schuilenburg 3, 4041 BK Kesteren, the Netherlands, to which address correspondence should be addressed.     

Diagnostics,genetic diversity and pathogenic variation of ascochyta blight of cool season food and feed legumes

Molecular diagnostic techniques have been developed to differentiate the Ascochyta pathogens that infect cool season food and feed legumes, as well as to improve the sensitivity of detecting latent infection in plant tissues. A seed sampling technique was developed to detect a 1% level of infection by Ascochyta rabiei in commercial chickpea seed. The Ascochyta pathogens were shown to be genetically diverse in countries where the pathogen and host have coexisted for a long time. However, where the pathogen was recently introduced, such as A. rabiei to Australia, the level of diversity remained relatively low, even as the pathogen spread to all chickpea-growing areas. Pathogenic variability of A. rabiei and Ascochyta pinodes pathogens in chickpea and field pea respectively, appears to be quantitative, where measures of disease severity were based on aggressiveness (quantitative level of infection) rather than on true qualitative virulence. In contrast, qualitative differences in pathogenicity in lentil and faba bean genotypes indicated the existence of pathotypes of Ascochyta lentis and Ascochyta fabae. Therefore, reports of pathotype discrimination based on quantitative differences in pathogenicity in a set of specific genotypes is questionable for several of the ascochyta-legume pathosystems such as A. rabiei and A. pinodes. This is not surprising since host resistance to these pathogens has been reported to be mainly quantitative, making it difficult for the pathogen to overcome specific resistance genes and form pathotypes. For robust pathogenicity assessment, there needs to be consistency in selection of differential host genotypes, screening conditions and disease evaluation techniques for each of the Ascochyta sp. in legume-growing countries throughout the world. Nevertheless, knowledge of pathotype diversity and aggressiveness within populations is important in the selection of resistant genotypes.     

Characterization of Sclerotinia minor populations in Texas peanut fields

In the summer of 2004 an epidemic of sclerotinia blight of peanut, a disease caused by Sclerotinia minor, occurred in Texas in fields where the disease was never previously detected. The disease was observed on many plants within one of the fields (>3000 disease foci), although most foci were <1 m. It is hypothesized that these observations were inconsistent with the recent introduction of a monocyclic pathogen, even if disease developed under conducive environmental conditions. The pattern of disease is most suggestive of the presence of foliar (ascospore) infections, although air temperature was above the known limits for apothecia development if the pathogen had arrived in the field in 2004 peanut seed. To further examine this epidemic, 232 isolates were collected, across a variety of spatial scales spanning this field and other Texas peanut fields, and evaluated for aggressiveness, fungicide sensitivity and genotypic diversity. There was wide variation among isolates for the phenotypic characteristics measured, but there was no evidence that a genotypically unique, highly aggressive, and fungicide resistant isolate had been introduced or evolved. The predominant genotype, TX1, which contained 154 isolates, was found in every county and field population.     

Tumours of Begonia and some other ornamentals,induced by Corynebacterium fascians

In 1975 many tumours were observed in plants ofBegonia Schwabenland grown in Aalsmeer. Submersion of the roots ofNicotiana megalosiphon seedlings in a homogenate of tumorous tissue, induced tumours after two weeks. Short periods of submergence yielded results similar to those obtained after longer periods. Tumour homogenates lost their infectivity after ten min at 50°C. Aphids transmitted the infectious agent.Treatment with propylene oxide did not inhibit infectivity completely. Filtration through a 450 nm filter removed the infectious agent.Tobacco tumor virus or a viroid could not be isolated. Cultures ofCorynebacterium fascians, isolated from tumours ofN. megalosiphon were highly infectious and induced tumours in healthyN. megalosiphon andBegonia. Tumorous tissue homogenates ofPelargonium zonale, Dahlia sp.,Gladiolus sp., andLilium sp. also caused tumours inN. megalosiphon, from whichC. fascians was isolated. It was not possible to produce tumours inN. megalosiphon with homogenates from roses with symptoms of bud proliferation.Samenvatting In 1975 werden vele tumoren waargenomen inBegonia Schwabenland op Aalsmeerse bedrijven (Fig. 1). De infectiositeit van tumorweefsel kon goed en snel worden vastgesteld door de wortels van zaailingen vanNicotiana megalosiphon in een homogenaat van tumorweefsel te dompelen. Tumoren ontstonden na twee weken, de eindbeoordeling geschiedde na een maand (Fig. 2). Ook verschillende andereNicotiana spp.,Melilotus officinalis (Fig. 3) enPisum odoratum (Fig. 4) werden aangetast.Bij de infectiositeitstoets gaven zeer korte dompeltijden even goede resultaten als langere (Tabel 1). Infectieus sap verloor zijn infectievermogen na 10 min verhitting bij 50°C. Bladluizen brachten de smetstof over. Propyleenoxide verminderde de infectiositeit wel, doch onderdrukte deze niet totaal. Bij filtratie door een 450 nm filter bleef het infectieuse agens op het filter achter. Het tumor-inducerende agens was ook aanwezig in die delen van planten met tumoren welke gezond leken en het ging voor een gering deel over met zaad (Tabel 2).Uit tumoren konden wij geen tabakstumorvirus of een viroïde isoleren. Culturen vanCorynebacterium fascians, geïsoleerd uit tumoren vanN. megalosiphon bleken zeer infectieus en veroorzaakten tumoren inN. megalosiphon enBegonia. Homogenaten van tumorweefsel vanPelargonium zonale, dahlia (Fig. 5), gladiool (Fig. 6) enLilium Mid Century Hybrid Enchantment (Fig. 7) veroorzaakten ook tumoren opN. megalosiphon, waaruitC. fascians werd geïsoleerd. Met sap van kroeskopzieke rozen konden wijN. megalosiphon niet besmetten.