Performance of different rubber tree clones against South American leaf blight (Microcyclus ulei)

This study was aimed to evaluate the behaviour of eighteen clones of Hevea brasiliensis (rubber) against South American leaf blight and to study progress of the disease. The experiment was conducted in Cachoeiro de Itapemirim‐ES, where 18 clones were evaluated. The experimental design was a randomized block with four replicates; each experimental unit consisted of three plants. Evaluations were performed at 15‐day intervals on 30 leaflets per tree. Disease incidence was quantified and infection classified according to the stages of development and type of damage. Leaf blight occurred during the entire experimental period; however, disease intensity varied with the resistance level of the clones and the time of year. Clones FX 3864, RRIM 725, RRIM 711, IAC 300 and IAN 873 exhibited the highest resistance to leaf blight.     

橡胶树砧木家系苗期生长性状变异分析

在西双版纳采集优良无性系RRIM600、GT1和IAN873开放授粉种子(花粉来源复杂),繁殖成3个家系用作砧木试验,对其实生苗木逐株编号,在嫁接前测量6个性状指标并进行变异分析。结果表明,在家系内株高、地径、每蓬叶数、大叶柄长、小叶长、小叶宽等6个性状变异系数为11.19%~27.17%;IAN873种子苗木平均株高为222.14 cm,平均地径为22.48 mm,显著大于RRIM600和GT1种子苗木的株高和地径(P<0.01);3个家系苗木株高和地径基本符合正态分布,株高和地径与叶子4个性状相关密切。橡胶树的这3个家系间和家系内单株间都存在丰富的遗传变异。     

云南种植橡胶树品种性状传递遗传分析

以云南植胶区推广种植的橡胶树品种为材料,根据各个品种的试验测定数据和生产中的性状表现,结合亲本特点,分析了橡胶树产量和抗辐射型低温能力两个性状在亲子间的传递关系。结果表明云南推荐品种的51%和自育品种的64%是GT1、PR107、RRIM600和PB86的后裔,GT1和PR107一般配合力高,GT1×PR107特殊配合力高,以在当地规模化种植具有较高重复力的无性系为育种亲本能获得较大遗传增益。要培育更好的地方品种,宜在当地选择符合育种目标的优树经过无性系重复力鉴定后用作育种材料。     

对橡胶树不同品种实行新割制后存在状况的调查与思考

对云南景洪农场六作业区橡胶树RRIM600、PR107、GT1、云研1号等品种实行刺激割胶新割制后的存在状况进行调查,结果表明:胶树的病残株率随着割龄的增加而增高,在开割7年后现存株率、有效割株率及单位面积有效割株数随着树龄的增加而降低;RRIM600在所有割龄段中,相对于其它参试品种表现出较强的适应性,存株率、有效割株率及单位面积有效割株数明显较高、死皮明显较轻。研究提出应在更大范围去深入调查,再认识RRIM600的优良性状,作出新的评价,合理地应用于生产。     

Seasonal variation in photochemical activity and hardiness in clones of Norway spruce (Picea abies)

We investigated changes in photochemical activity and cold hardiness of detached needles of three clones of Picea abies (L.) Karst. by measuring variable chlorophyll fluorescence (F(v)/F(m)), before and after artificial freezing, from September to June. Photochemical activity varied considerably during the study, but only minor differences in photochemical activity among the clones were observed before freezing. Photochemical activity was high during early fall and then declined from November until April. Photochemical activity was at a minimum in April and then increased quickly to high values in May. During the period from late September to October, and also during the winter, differences in F(v)/F(m) ratios after artificial freezing to below -10 degrees C were observed among clones, indicating clonal differences in cold hardiness and hardiness development. The clone having an average height of 2.3 m after 11 years showed consistently lower cold hardiness than clones that had reached average heights of 4.0 and 5.0 m. There were also differences in the temperature requirement for bud flushing among clones.     

Evaluation of Brazilian wild Hevea germplasm for cold tolerance:genetic variability in the early mature growth

Hevea brasiliensis is a commercially cultivated species for its natural rubber(NR) latex in South East Asian countries. To meet the ever-increasing demand, NR cultivation has been extended to non-traditional regions due to the limited scope of further expansion in traditional rubbergrowing areas in India. These areas are often confronted with various abiotic stresses, especially high and low temperatures, which cause reduction in plant growth, thereby increasing its uneconomical immaturity period. Eighteen wild Hevea accessions along with two modern clones RRII203 and PB 235 and two check clones RRIM 600 and Haiken 1 were evaluated in the early mature growth phase.The site was at Nagrakata, West Bengal, the sub-Himalayan cold-prone region of India. In Hevea, crop production is governed by two major factors, growth-vigor and production capacity. Growth-vigor is of special importance because the production of rubber is a process linked with the early growth of the plant, which results in early tappability and early economic gains. The genotypes exhibited highly significant clonal differences(P = 0.05) for all the growth traits. Tappability percentage in the seventh year, ranged from 0.33 %(AC 3074, AC 3075, AC 3293) to 89.67 %(RO 2727). The most vigorously growing accession(RO2727) reached tappabile girth early in the seventh year when the girth of plant ranged from 22.38 cm(AC 3293) to53.12 cm(RO 2727). The general mean was 43.32 cm, and the similar growth trend was exhibited by these accessions in the tenth year also. Annual girth increment(cm a-1) over3 years ranged from 1.81 cm(AC 3075) to 6.80 cm(RO2727). The mean winter girth increment(cm a-1) over4 years ranged from 0.13 cm(AC 3075) to 0.96 cm(RO2727) as compared to the check clone RRIM 600(1.11 cm)and Haiken 1(1.10 cm). Wide differences between the phenotypic coefficient of variation(50.29) and genotypic coefficient of variation(24.82) were observed for winter girth increment. Girth in the tenth year recorded the highest heritability(87 %). Girth was significantly correlated with the other growth traits. The top 30 % of the potential accessions showing high growth vigour and early tappability under cold stress were identified. These ecotypes/selections have high potential value for the development of coldtolerant clones for these regions and also in broadening the genetic base of the present-day cultivated rubber.     

Impact of snow cover on photoinhibition and winter dessication in evergreen Rhododendron ferrugineum leaves during subalpine winter

Effects of winter snow cover on photoinhibition and possible interactions with winter desiccation were investigated in situ in an evergreen subalpine woody species, Rhododendron ferrugineum L., at the alpine timberline (1950 m a.s.l.). Timing and duration of complete snow cover markedly influenced potential efficiency of photosystem II (PSII; F(v) /F(m)). Lack of snow cover led to severe but mostly reversible photoinhibition with F(v)/F(m) values as low as 0.05. Complete snow cover immediately stopped further reductions in PSII efficiency. Snow cover promoted recovery from photoinhibition, but only if, in addition to shading by snow, plants were exposed to nonfreezing temperatures close to 0 degrees C. The F(v)/F(m) ratio was closely related to minimum leaf temperatures because both photoinhibition and recovery from photoinhibition were strongly influenced by temperature. The period without major reductions in PSII efficiency lasted for only two months, reflecting the extremely short growing period in the subalpine environment. Compared with complete snow cover, incomplete snow cover led to significantly higher water losses as well as lower dehydration tolerance, because both osmotic adjustment and changes in turgor maintenance capacity were significantly reduced. Interactions between photoinhibition and winter desiccation were masked by the direct effects of freezing temperatures. However, both photoinhibition and winter desiccation are closely linked and occur together under field conditions in this evergreen subalpine woody species.     

Intra- and inter-clonal tree growth variations of Hevea brasiliensis

We evaluated the effects of planting densities (500, 1,000,1,500 and 2,000 trees·ha-1) on tree growth performance (diameter atbase, diameter at breast height, and clear bole height) of two clones(RRIM 2020 and RRIM 2025) of nine years old plantations of rubber tree(Hevea brasiliensis Muell. Arg) in Malaysia. For the four planting densities of the two clones, basal area and diameter at breast height declined with increasing planting density. Clear bole heights were greatest at 1,500 trees·ha-1 and lowest at 500 trees·ha-1 for the clone RRIM 2020, andat 2,000 trees·ha-1 and 500 trees·ha-1 for clone RRIM 2025. We conclude that the ideal planting density is 2,000 trees·ha-1 for obtaining high volume of wood production and 500 trees·ha-1 for high wood quality.     

Application of chlorophyll fluorescence to evaluate Mn tolerance of deciduous broad-leaved tree seedlings native to northern Japan

We used chlorophyll fluorescence to examine photosynthetic responses to excess Mn accumulation in leaves of four tree species differing in successional traits. Betula ermanii Cham. (Be) and Alnus hirsuta Turcz. (Ah) were studied as representatives of early-successional species. Ulmus davidiana Planch. var. japonica (Rehder) Nakai (Ud) was selected as a mid-successional species, and Acer mono Maxim. var. glabrum (Lév. et Van't.) Hara (Am) was chosen as a late-successional species. In Be, Ah and Am, high foliar concentrations of Mn had little effect on maximum photochemical efficiency of photosystem II (PSII), as indicated by the values of dark-adapted F(v)/F(m), whereas a significant decrease was observed in Ud. Photochemical quenching (qP) and the excitation capture efficiency of open PSII (F'(v)/F'(m)) decreased with increasing leaf Mn concentration at photosynthetic steady state after a 15-min exposure to 430 &mgr;mol m(-2) s(-1) PPFD. Compared with early-successional species, these decreases were greater in mid- and late-successional species leading to lower effective quantum efficiencies of PSII (DeltaF/F'(m) = qP x F'(v)/F'(m) = (F'(m) - F)/F'(m)). To determine the extent of photoinhibition, F(v)/F(m) of the illuminated leaves was remeasured after a 15-min dark period. Compared with the dark-adapted F(v)/F(m), we observed a significant decrease in F(v)/F(m) in Am leaves containing high concentrations of Mn. These chlorophyll fluorescence studies indicate that the early-successional species Be and Ah have a higher tolerance to excessive accumulations of Mn in leaves than the mid- and late-successional species Ud and Am.     

东部白松叶片光合作用日变化特征的研究

利用LI-6400便携式光合仪观测了东部白松自然状态的叶片光合作用日变化规律。结果表明:东部白松叶片的净光合速率日变化呈典型双峰曲线,表现出明显的光合"午睡"现象,光合效率午间明显降低;蒸腾速率日变化趋势与净光合速率相似,但两者最大值出现的时间不相同。气孔导度日变化与净光合速率日变化相似,但并不完全同步,气孔导度最大值出现的时间较净光合速率提前约2h。     

Seasonal acclimation of photosystem II in Pinus sylvestris. I. Estimating the rate constants of sustained thermal energy dissipation and photochemistry

Acclimation of the partitioning of absorbed light energy in Photosystem II (PSII) between photochemical and non-photochemical processes includes short-term adjustments that are rapidly reversed in the dark and seasonal acclimation processes that are unaffected by dark acclimation. Thus, by using dark-acclimated leaves to study the seasonal acclimation of PSII, the confounding effect of short-term adjustments is eliminated. The maximum quantum yield of photochemistry, estimated by chlorophyll fluorescence analysis as F(v)/F(m), where F(v) = (F(m) - F(o)), and F(m) and F(o) are maximum and minimum chlorophyll fluorescence, respectively, has been widely used to follow the seasonal acclimation of PSII, because it is measured in dark-acclimated leaves. Seasonal changes in F(v)/F(m) can be caused by adjustments in either the photochemical capacity in PSII, or the capacity of thermal dissipation in PSII, or both. However, there is a lack of chlorophyll fluorescence parameters that can distinguish between these processes. In this study, we introduce two new parameters: the rate constants of sustained thermal energy dissipation (k(NPQ)) and of photochemistry (k(P)). We estimated k(NPQ) and k(P) from dark-acclimated F(o) and F(m) measured during spring recovery of photosynthesis in Scots pine (Pinus sylvestris L.) trees. We suggest that k(NPQ) and k(P) be used to study the mechanisms underlying the observed seasonal acclimation in PSII, because these parameters provide quantitative data that complement and extend F(v)/F(m) measurements.     

不同灌溉条件对刺槐生长量及各项生理指标的影响

在小管出流与大水漫灌等不同灌溉方式下,研究刺槐的相对含水量(RWC),水分饱和亏缺(RWD)、光响应曲线、净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、胞间CO2浓度(Ci)、单叶水分利用效率(WUE)等指标的变化。结果表明,小管出流灌溉条件下,刺槐叶片净光合速率Pn值、蒸腾速率、气孔导度、生长量均大于漫灌下刺槐;但是,单叶水分利用率与叶片胞间CO2浓度在这2两种灌溉条件下差异较小。     

金森女贞光合生理特性的研究

研究了金森女贞光合生理日变化及其光补偿点和饱和点.结果表明:金森女贞的单日净光合速率(Pn)呈双峰曲线,出现"午休"现象,造成Pn下降的主要原因是非气孔因素.蒸腾速率(Tr)和气孔导度(Gs)的变化趋势与Pn相似,胞间CO2浓度(Ci)与之相反.Pn与Ci和PAR的相关性达到极显著水平,Tr与PAR、RH和VPD均达到极显著水平.金森女贞的光补偿点(LCP)为16.67 μmol/(m2·s),饱和点(ISP)约为1 000 μmol/(m2·s),属于阳性植物.     

Seasonal variation in photosystem II efficiency and photochemical reflectance index of evergreen trees and perennial grasses growing at low and high elevations in subtropical Taiwan

Three pines species, three evergreen broadleaf trees, one C(3) and two C(4) perennial grasses of subtropical Taiwan were studied to elucidate the correlation between photosystem II (PSII) efficiency and photochemical reflectance index (PRI = (R(531) - R(570))/(R(531) + R(570))). Measurements were made at two sites differing in altitude (800 and 2600 m) over several growing seasons. At high elevation, potential PSII efficiency, measured by chlorophyll fluorescence (the ratio of variable to maximal fluorescence; F(v)/F(m)) at predawn, decreased with decreasing air temperature and varied greatly among species. At the lowest air temperature (-3 degrees C) studied, variation in F(v)/F(m) among species ranged from 0.33 to 0.72. In contrast, at low elevation where air temperature was moderate, seasonal variation in F(v)/F(m) was small in all of the study species. When species, elevation and season data were pooled, despite the high variation in F(v)/F(m) among species, a good correlation between F(v)/F(m) and PRI was observed. When compared at the same value of PRI, F(v)/F(m) of evergreen trees was higher than that of perennial grasses; however, when the minimum temperature on the measurement day was below 0 degrees C, F(v)/F(m) was underestimated relative to PRI. We conclude that PRI could be used as a remote indicator of photosynthetic function when air temperature is above 0 degrees C.     

5种四倍体泡桐生长特性的研究

以2年生5种四倍体泡桐为材料，研究其光合作用、材积生长量、接干长度、比叶重（ SLW）等指标间的差异。结果表明，5种四倍体泡桐叶片的净光合速率（ Pn）、气孔导度（ Gs）、蒸腾速率（ Tr）、胞间CO2浓度（ Ci）光合因子的日变化规律相同。9月份叶片的Pn、 Gs、 Tr、 Ci日变化为单峰曲线，四倍体豫杂一号泡桐（ TF4）的最大净光合速率高于其余4个种。此外，四倍体毛泡桐（ T4）的树高生长量和接干高增长量、 TF4的胸径生长量和比叶重增长量、四倍体南方泡桐（ A4）的材积增长量最大。5种四倍体泡桐的树高、胸径、接干、材积的相关性表明，材积与胸径呈正相关关系，相关性显著；树高与比叶重呈正相关关系，但相关性不显著。     

早实核桃‘农核1号’的光合特性

【目的】了解早实核桃新品种‘农核1号’的光合特性,为该品种优质高效栽培提供参考。【方法】以早实核桃‘农核1号’为试材,采用Li 6400测定叶片净光合速率(P_n)、蒸腾速率(T_r)、气孔导度(G_s)、胞间CO₂浓度(C_i)、水分利用效率(WUE)、气孔限制值(L_s)及主要环境因子的日变化,分析各指标间的关系。【结果】‘农核1号’叶片P_n日变化呈单峰曲线,最高峰出现在9:00,峰值达15.67 μmol/(m²·s)。11:00—19:00,P_n降低主要受非气孔限制因素的影响。一天当中P_n及其他光合参数在不同时段存在一定差异。相关分析结果表明,P_n与T_r在13:00—19:00呈极显著正相关;P_n与G_s在各时段均呈极显著正相关;P_n与C_i在7...