Influence of rice varieties,nitrogen management and planting methods on methane emission and water productivity

A field experiment was conducted during rainy seasons of 2009 and 2010 at New Delhi, India to study the influence of varieties and integrated nitrogen management (INM) on methane (CH₄) emission and water productivity under flooded transplanted (FT) and aerobic rice (AR) cultivation. The treatments included two rice (‘PB 1’ and ‘PB 1121’) varieties and eight INM practices including N control, recommended dose of N through urea, different combinations of urea with farmyard manure (FYM), green manure (GM), biofertilizer (BF) and vermicompost (VC). The results showed 91.6–92.5 % lower cumulative CH₄ emission in AR compared to FT rice. In aerobic conditions, highest cumulative CH₄ emission (6.9–7.0 kg ha^?1) was recorded with the application of 100 % N by organic sources (FYM+GM+BF+VC). Global warming potential (GWP) was significantly lower in aerobic rice (105.0–107.5 kg CO₂ ha^?1) compared to FT rice (1242.5–1447.5 kg CO₂ ha^?1). Significantly higher amount of water was used in FT rice than aerobic rice by both the rice varieties, and a water saving between 59.5 and 63 % were recorded. Under aerobic conditions, both rice varieties had a water productivity of 8.50–14.69 kg ha^?1, whereas in FT rice, it was 3.81–6.00 kg ha^?1. In FT rice, a quantity of 1529.2–1725.2 mm water and in aerobic rice 929.2–1225.2 mm water was used to produce one kg rice. Thus, there was a saving of 28.4–39.6 % total water in both the rice varieties under AR cultivation.     

The effect of floating vegetation on CH<Subscript>4</Subscript> and N<Subscript>2</Subscript>O emissions from subtropical paddy fields in China

Duckweed (Lemna minor), a floating macrophyte belonging to the Lemnaceae family, is commonly found in subtropical paddy fields. This plant rapidly takes up nutrients from water and forms dense floating mats over the water surface that may impact the biogeochemical processes and greenhouse gas production in paddy fields. In this study, we measured CH₄ and N₂O emissions from duckweed and non-duckweed plots in a subtropical paddy field in China during the period of rice growth using static chamber and gas chromatography methods. Our results showed that CH₄ emission rate ranged from 0.19 to 26.50 mg m^?2 h^?1 in the duckweed plots, and from 1.02 to 28.02 mg m^?2 h^?1 in the non-duckweed plots. The CH₄ emission peak occurred about 1 week earlier in the duckweed plots compared to the non-duckweed counterparts. The mean CH₄ emission rate in the duckweed plots (9.28 mg m^?2 h^?1) was significantly lower than that in non-duckweed plots (11.66 mg m^?2 h^?1) (p < 0.05), which might be attributed to the higher water and soil Eh in the former. N₂O emission rates varied between ?50.11 and 201.82 µg m^?2 h^?1, and between ?28.93 and 54.42 µg m^?2 h^?1 in the duckweed and non-duckweed plots, respectively. The average N₂O emission rate was significantly higher in the duckweed plots than in the non-duckweed plots (40.29 vs. 11.93 µg m^?2 h^?1) (p < 0.05). Our results suggest that the presence of duckweed will reduce CH₄ emission, but increase N₂O flux simultaneously. Taking into account the combined global warming potentials of CH₄ and N₂O, we found that growing duckweed could reduce the overall greenhouse effect of subtropical paddy fields by about 17 %.     

Pattern of methane emission and water productivity under different methods of rice crop establishment

Methane (CH₄) emission and water productivity were estimated in an experiment conducted during wet (rainy) season of 2010 at the research farm of Indian Agricultural Research Institute, New Delhi, India. Treatments comprising three methods of crop establishment viz., conventional transplanting (CT), system of rice intensification (SRI) and double transplanting (DT) were laid out in randomized block design with four replications. Scented rice (Oryza sativa L) variety ‘Pusa Basmati 1401’ was transplanted in puddle field. In CT and SRI 21 and 12-day-old seedlings, respectively, were transplanted while in DT overall 45-day-old seedlings were transplanted. In CT and DT flooded conditions while in SRI saturated conditions were maintained. Results indicated that among the methods of crop establishment, CT had maximum cumulative CH₄ emission (32.33 kg ha^?1) followed by DT (29.30 kg ha^?1) and SRI (19.93 kg ha^?1). Temporal CH₄ flux fluctuated between 79.7 and 482.0 mg m^?2 day^?1 under CT; 46.0 and 315.0 mg m^?2 day^?1 in SRI and 86.7 and 467.3 mg m^?2 day^?1 in DT. Considerable temporal variations in the individual CH₄ fluxes were observed. Flux of CH₄ was generally higher in early stage of crop and peaked about 21 days after transplanting coinciding with tillering stage of crop. CH₄ flux declined gradually from 75 days after transplanting and stabilized at the harvest stage of rice in all the three methods of transplanting. Global warming potential was highest in CT (807.4 kg CO₂ ha^?1) and lowest in SRI (498.25 kg CO₂ ha^?1). However, a reverse trend was observed with carbon efficiency ratio. The water savings to the extent of six irrigations was recorded in SRI over CT. A saving of 27.4 % irrigation water and 18.5 % total water was recorded in SRI over CT while the corresponding values of DT over CT were 14.5 and 9.8 %. Water productivity of SRI (3.56 kg/ha mm) was significantly higher as compared to DT (2.87 kg/ha mm) and CT (2.61 kg/ha mm).     

Effect of organic,inorganic and slow-release urea fertilisers on CH<Subscript>4</Subscript> and N<Subscript>2</Subscript>O emissions from rice paddy fields

Vietnam is one of the world’s top two rice exporting countries. However, rice cultivation is the primary source of agriculture’s greenhouse gas (GHG) emissions in Vietnam. In particular, strategies are required to reduce GHG emissions associated with the application of organic and inorganic fertilisers. The objective of this study was to assess the effects of various combinations of biochar (BIOC), compost (COMP) and slow-release urea (SRU) on methane (CH₄) and nitrous oxide (N₂O) emissions. In total, 1170 gas samples were collected from closed gas chambers in rice paddies at Thinh Long commune and Rang Dong farm in northern Vietnam between June and October 2014. The gas samples were analysed for CH₄-C and N₂O-N fluxes using gas chromatography. The application of BIOC alone resulted in the lowest CH₄ emissions (4.8–59 mg C m^?2 h^?1) and lowest N₂O emissions (0.15–0.26 µg N m^?2 h^?1). The combined application of nitrogen–phosphorus–potassium (NPK) + COMP emitted the highest CH₄ (14–72 mg C m^?2 h^?1), while ½NPK + BIOC emitted the highest N₂O (1.03 µg N m^?2 h^?1 in the TL commune), but it was the second lowest (0.495 µg N m^?2 h^?1) in the RD farm. Green urea and orange urea reduced N₂O emissions significantly (p < 0.05) compared to white urea, but no significant differences were observed with respect to CH₄ emissions. SRU fertilisers and BIOC alone measured the lowest greenhouse gas intensity, i.e. <2.5 and 3 kg CO₂ eq. kg^?1 rice grain, respectively. Based on these results, application of fertilisers in the form of BIOC and/or orange or green urea could be a viable option to reduce both CH₄ and N₂O emissions from rice paddy soils.     

Influence of irrigation frequency on greenhouse gases emission from a paddy soil

Water management is known to be a key factor on methane (CH₄), carbon dioxide (CO₂), and nitrous oxide (N₂O) emissions from paddy soils. A field experiment was conducted to study the effect of continuous irrigation (CI) and intermittent irrigation (II) on these emissions. Methane, CO₂, and N₂O emissions from a paddy soil were sampled weekly using a semi-static closed chamber and quantified with the photoacoustic technique from May to November 2011 in Amposta (Ebro Delta, NE Spain). Intermittent irrigation of rice paddies significantly stimulated (N₂O + N₂)–N emission, whereas no substantial N₂O emission was observed when the soil was re-wetted after the dry phase. The cumulative emission of (N₂O + N₂)–N was significantly larger from the II plots (0.73 kg N₂O–N ha^?1 season^–1, P < 0.05) than from the CI plots (?1.40 kg N₂O–N ha^?1 season^?1). Draining prior to harvesting increased N₂O emissions. Draining and flooding cycles controlled CO₂ emission. The cumulative CO₂ emission from II was 8416.35 kg CO₂ ha^?1 season^?1, significantly larger than that from CI (6045.26 kg CO₂ ha^?1 season^?1, P < 0.05). Lower CH₄ emission due to water drainage increased CO₂ emissions. The soil acted as a sink of CH₄ for both types of irrigation. Neither N₂O–N nor CH₄ emissions were affected by soil temperature. Global warming potential was the highest in II (4738.39 kg CO₂ ha^?1) and the lowest in CI (3463.41 kg CO₂ ha^?1). These findings suggest that CI can significantly mitigate the integrative greenhouse effect caused by CH₄ and N₂O from paddy fields while ensuring the highest rice yield.     

Super Rice Cropping Will Enhance Rice Yield and Reduce CH4 Emission： A Case Study in Nanjing,China

A pot experiment was performed to learn the differences in plant productivity and OH4 emission between two rice cultivars, super rice variety Ningjing 1 and traditional variety Zhendao 11, which were currently commercially appUed in Nanjing, China. Similar seasonal changes of CH4 emission fluxes and soil solution CH4 contents were found between the tested cultivars. Although there was no significant difference in plant biomass production between the cultivars, the grain yield of Ningjing 1 was significantly higher by 35.0% （P 〈 0.05） than that of Zhendao 11, whereas the total CH4 emission from Ningjing 1 was 35.2% lower （P 〈 0.05）. The main difference in the amounts of CH4 emission between the cultivars occurred in the period from the tillering stage to the heading stage. The biomass-scaled and yield-scaled CH4 emissions were respectively 3.8 and 5.2 mg/g for Ningjing 1, significantly lower than those for Zhendao 11 （7.4 and 12.8 mg/g, respectively）. According to the relationships between the plant growth characteristics and the CH4 emission, a stronger root system contributed mainly to the lower CH4 emission of Ningjing 1, as compared with Zhendao 11. Our results demonstrated that super rice has advantages not only in grain productivity but also in CH4 emission mitigation. Further expansion of super rice cropping will enhance rice yield and reduce greenhouse gas emission in China.     

Methane emission from irrigated rice ecosystem: relationship with carbon fixation,partitioning and soil carbon storage

Rice is a major agricultural crop and accounts for 40 % of the total food grain production of India. A field experiment was conducted for two successive seasons (December–June, 2012–13 and December–June, 2013–14) to assess the efficiency of rice varieties for methane (CH₄) emission in relation to atmospheric carbon fixation, partitioning of carbon, and storage in the soil. Six high yielding rice varieties, Bahadur, Cauvery, Dinanath, Joymoti, Kanaklata, and Swarnabh were grown under irrigated condition. Results of the present investigation depicted differences in photosynthetic rate among the varieties accompanied by differential ability for plant biomass partitioning between the shoots and the roots. Stomatal frequency of flag leaf at panicle initiation stage was found to have strong influence on photosynthesis. Low CH₄-emitting rice varieties, Bahadur and Dinanath, were found to have lower size of the xylem vessels than the high CH₄-emitting rice varieties, Joymoti and Kanaklata, and found to influence the CH₄ flux. Soil organic carbon storage of 0.505 Mg C ha^?1 y^?1 in the plough layer of soil (0–15 cm) confirmed that irrigated rice ecosystem is an effective sink of carbon. These findings suggest that selection of suitable rice varieties with higher photosynthetic efficiency and lower emission of CH₄ can be a suitable biological mitigation of this greenhouse gas. Although an inverse relationship of CH₄ with carbon dioxide (CO₂) efflux was observed, irrigated rice ecosystem has a good potential to store substantial amount of carbon in the soil.     

In vitro rumen methane output of perennial ryegrass varieties and perennial grass species harvested throughout the growing season

The selection and feeding of perennial ryegrass (Lolium perenne L.) varieties (PRV) or perennial grass species (PGS) may affect enteric methane (CH₄) output because of changes in the fermentation dynamics in the rumen as a result of differences in herbage chemical composition. The objective of this study was to determine the effects of PRV and PGS harvested throughout the growing season on herbage chemical composition, and in vitro rumen fermentation variables and CH₄ output per unit of feed using a batch culture technique. Seven PRV (Experiment 1: Alto, Arrow, Bealey, Dunluce, Greengold, Malone, Tyrella) and six perennial grasses [Experiment 2: perennial ryegrass (Navan), perennial ryegrass (Portstewart), cocksfoot, meadow fescue, tall fescue, timothy; defined as PGS], managed under a simulated grazing regime, were incubated for 24 h with buffered rumen fluid in two separate experiments. The CH₄ output per unit of feed dry‐matter (DM) incubated was not affected (P > 0·05) by PRV (range of mean values across PRV of 23·9–25·3 (SEM 0·41) mL g^?1 DM) or by PGS (25·6–26·6 (SEM 0·37) mL g^?1 DM). The CH₄ output per unit feed DM disappearing during the in vitro rumen incubation was not affected by PRV (33·9–35·1 (SEM 0·70) mL g^?1 DM), and although there was an overall PGS effect (P < 0·05; 37·2–40·3 (SEM 0·71) mL g^?1 DM), none of the paired contrasts between PGS were significant when analysed using Tukey adjusted comparisons. This outcome reflected either small‐scale or a lack of treatment effects on individual herbage chemical composition (e.g. 454–483 g NDF kg^?1 DM, 215–224 g CP kg^?1 DM and 94–122 g water‐soluble carbohydrate (WSC) kg^?1 DM across PRV; 452–506 g NDF kg^?1 DM, 208–243 g CP kg^?1 DM and 73–131 g WSC kg^?1 DM across PGS) and in vitro rumen fermentation variables. Hence, these results provide no encouragement that choices among the grasses examined, produced within the management regimes operated, would reduce enteric CH₄ output per unit of feed in vivo. However, the technique utilized did not take account of animal × PRV or PGS interactions, such as potential differences in intake between animals, that may occur under farm conditions.     

Comparison of feed intake,body weight gain,enteric methane emission and relative abundance of rumen microbes in steers fed sainfoin and lucerne silages under western Canadian conditions

Beef cattle are major contributors of enteric methane (CH₄) emissions in Canada. Feeding forages containing condensed tannins (CT) has been suggested as a means of reducing enteric CH₄ emissions and improving production efficiency. Sainfoin (Onobrychis viciifolia) is one of the CT‐containing legumes, which has also been recognized to have several additional beneficial properties. This study compared sainfoin silage (SS) and lucerne (Medicago sativa) silage (LS) with respect to animal performance, enteric CH₄ production, abundance of selected rumen microbes and selected serum parameters in yearling steers during a Canadian winter. Sainfoin silage in our study contained approximately 12 g CT kg^?1 dry matter (DM), a level which did not adversely affect silage palatability as steers fed SS experienced higher DM intake. However, animals fed LS and SS had similar body weight gain over the course of the trial. Feeding SS resulted in lower blood urea nitrogen concentrations and relative abundance of methanogenic archaea in rumen fluid. Yet, microbial population shifts fell short of exerting significant influence on enteric CH₄ emissions. This study suggests that under western Canadian growing and animal‐rearing conditions, sainfoin is not yet competitive with lucerne forage with respect to enteric CH₄ emissions or animal productivity warranting further research and development.     

In vitro rumen methane output of grasses and grass silages differing in fermentation characteristics using the gas‐production technique (GPT)

The chemical composition of silage consumed by cattle can influence the subsequent rumen microbial fermentation patterns and methane (CH₄) emissions. The objectives of this study were to (i) evaluate the effect of ensilage on the in vitro rumen methane output of perennial ryegrass and (ii) relate the silage fermentation characteristics of grass silages with in vitro rumen methanogenesis. Three pre‐harvest herbage‐conditioning treatments and seven silage‐additive treatments were used in a laboratory‐scale silo experiment to produce a diversity of silage fermentation characteristics. Ensilage reduced (P < 0·01) the in vitro rumen CH₄ output (mL CH₄ g^?1 dry matter (DM) disappeared). This reflected differences in the direction of rumen fermentation (lower acetic (P < 0·05) and higher propionic (P < 0·001) acid proportions in volatile fatty acids) rather than major changes in the extent of in vitro rumen fermentation (i.e. mmol VFA g^?1 DM). The magnitude of the decrease in CH₄ output (mL g^?1 DM incubated) owing to ensilage increased as the extent of silage fermentation, and in particular the lactic acid concentration, increased. In contrast, among silages with relatively similar extents of silage fermentation (i.e. total fermentation products), an increase in the proportion of lactic acid in silage fermentation products led to a more extensive in vitro rumen fermentation and thus to a greater CH₄ output (mL g^?1 DM).     

Rice husk biochar application to paddy soil and its effects on soil physical properties,plant growth,and methane emission

The objective of this study was to investigate the effects of the application of rice husk biochar on selected soil physical properties, rice growth, including root extension, and methane (CH₄) emissions from paddy field soil. Three replication experiments were conducted using outdoor pot experiments utilizing commercial rice husk biochar mixed with paddy soil at a rate of 0 (control), 2, and 4 % (weight biochar/weight soil) in which the rice was cultivated for 100 days under a continuously flooded condition. The physical properties of soils were analyzed before and after the growing periods. Some parameters of rice growth and CH₄ emissions of paddy soils were monitored weekly during the experiment. Root extension was also analyzed after harvesting. The experiments showed that the application of rice husk biochar improved the physical properties of paddy soils. It led to a decrease in bulk density and an increase in saturated hydraulic conductivity, including the total pore volume as well as the available soil water content. The shoot height of rice plants was significantly higher in soil amended with 4 % biochar than that in the control soil. However, other plant growth parameters and root extension were only slightly affected by the application. It was also found that amending soil with biochar led to a reduction of the total CH₄ emissions by 45.2 and 54.9 % for an application rate of 2 and 4 %, respectively, compared with the control. Our results showed that the higher the application rate, the stronger the effect of biochar was observed. More research is still necessary for a better understanding of the underlying mechanisms.     

Dry direct-seeding of rice for mitigating greenhouse gas emission: field experimentation and simulation

Conventional puddled transplanted rice (TPR) is a major source of greenhouse gas (GHG), particularly methane, causing global warming. Direct-seeded rice (DSR) is a feasible alternative to mitigate methane emission, besides saving water and labor. A 2-year field experiment was carried out to quantify GHG mitigation and water- and labor-saving potentials of the DSR crop compared to TPR in three villages in Jalandhar district of Punjab, India. The InfoRCT simulation model was used to calculate the emission of CO₂ besides CH₄ and N₂O in different districts of Punjab, India. Total global warming potential (GWP) in transplanted rice in various districts of Punjab ranged from 2.0 to 4.6 t CO₂ eq. ha^?1 and in the DSR it ranged from 1.3 to 2.9 t CO₂ eq. ha^?1. Extrapolation analysis showed that if the entire area under TPR in the state is converted to DSR, the GWP will be reduced by 33 %, and if 50 % area is converted to DSR the GWP will be reduced by 16.6 % of the current emission. The DSR crop saved 3–4 irrigations compared to the transplanted rice without any yield penalty. Human labor use also reduced to 45 % and tractor use to 58 % in the DSR compared to TPR.     

Comparative efficacy of surface drying and re-drying seed priming in rice: changes in emergence, seedling growth and associated metabolic events

In seed priming, seeds are soaked in a solution of low water potential and dried back to their original weight. This study was conducted to evaluate the comparative performance of different seed priming treatments and to investigate whether re-drying is essential or not? Seeds of fine grain aromatic rice (Oryza sativa L.) cultivar Super-Basmati were subjected to hardening, osmohardening with CaCl₂ and KCl (ψ _s ?1.25 MPa) each for 24 h (one cycle), and hydropriming and ascorbate priming (10 mg L^?1) for 48 h. Seeds were primed in two sets. In one set after each treatment, seeds were given three surface washings with distilled water and dried back near to original moisture contents with forced air. In the other case, after surface washings with distilled water, seeds were surface dried using blotting paper and sown immediately. All the priming treatments improved the emergence, seedling growth and reserve metabolism. Use of surface drying was more effective for rice seed invigoration, as evident from earlier and synchronized seedling emergence. Moreover, shoot and root length, seedling dry weight, root score, α-amylase activity, soluble sugars and dehydrogenase activity were also improved, although germination percentage and leaf score were similar from both strategies. Results suggested that surface drying, rather than re-drying close to original weight, was more effective, while among the treatments, osmohardening with CaCl₂ was the most effective. Increasing rice production with judicious use of water is need of the day, and aerobic rice cultivation is an attractive alternative for this purpose. However, poor and erratic stand establishment is one of the major hindrances in its wide scale adoption. In this regard, seed priming techniques are pragmatic approaches to achieve proper stand establishment in the new rice culture. They help in improving seedling density per unit area under optimal and adverse soil conditions and may be opted to improve the performance of aerobic rice.     

Intermittent drainage in paddy soil: ecosystem carbon budget and global warming potential

Intermittent drainage of rice fields alters soil redox potential and contributes to the reduction of CH₄ emission and thus may reduce net global warming potential (GWP) during rice cultivation. Incorporation of green biomass helps maintaining soil organic matter, but may increase CH₄ emission. We investigated net ecosystem carbon budget (NECB) and net GWP under two water management regimes—continuous flooding and intermittent drainage—having four biomass incorporation levels (0, 3, 6 and 12 Mg ha^?1). Water management and biomass incorporation level demonstrated significant (P < 0.05) interaction effect on the NECB and GWP. Intermittent drainage decreased the NECB by ca. 6–46 % than continuous flooding under same rates of cover crop biomass (CCB) incorporation. Moreover, intermittent drainage reduced seasonal CH₄–C fluxes by ca. 54–58 % and net GWP by 35–58 % compared to continuous flooding. There was also no significant reduction in rice yield because of intermittent drainage under similar CCB. This implies that incorporation of 3 Mg ha^?1 CCB and intermittent drainage could be a good option for reducing net GWP and higher grain yield.     

Short-term diurnal and temporal measurement of methane emission in relation to organic carbon, phosphate and sulphate content of two rice fields of central Gujarat, India

Methane emission from two rice fields of Lambhvel village, Anand district, Central Gujarat, India, was measured for whole cultivation period during pre-summer season. Along with the methane emission, soil chemistry of the two rice fields (Organic Carbon, PO₄⁻² and SO₄⁻²) was determined. The methane emission ranged from 0.10 to 0.56 mg/m² per h, having maximum emission during noon period (11 a.m. to 1 p.m.) of the day at the Rice field-1. Besides, at rice field-2, the methane emission ranged between 0.15 and 0.94 mg/m² per h, having maximum peak during same period (11 a.m. to 1 p.m.) of the day. The results of the current investigation confirm that the methane emission vary substantially between two rice fields, and suggest that soil chemistry and water level might control the methane emission in both the rice fields and suppressed by the phosphate and sulphate concentrations. The greater methane emission was declined with the age of rice plantation. Correlation analysis, ANOVA and F test showed that the methane emission from both the sites has positive correlation with organic carbon and negative correlation with sulfate and phosphate content of the soil and the details of these reasons will be discussed in this paper.     

Emissions of CH4 and CO2 from paddy fields as affected by tillage practices and crop residues in central China

A field experiment was conducted to investigate effects of tillage practices [no-tillage (NT) and conventional intensive tillage (CT)] and oilseed rape residue returning levels (0, 3000, 6000, 9000 kg dry matter ha^?1) on methane (CH₄) and carbon dioxide (CO₂) emissions and grain yield from paddy fields during the 2011 rice growing season after 2 years oilseed rape-rice rotation in central China. The experiment was established following a split-plot design of a randomized complete block with tillage practices as the main plots and residue returning levels as the sub-plots. NT significantly decreased CO₂ and CH₄ emissions by 38.8 and 27.3 % compared with CT, respectively. Residue returning treatments released significantly more CO₂ and CH₄ by 855.5–10410 and 51.5–210.5 kg ha^?1 than no residue treatments, respectively. The treatments of 3,000 and 6,000 kg ha^?1 residue returning significantly increased rice grain yield by 37.9 and 32.0 % compared with the treatment of no residue returning, respectively. Compared with NT, CT increased yield-scaled emissions of CH₄ and CO₂ by 16.0 %. The treatments of 6,000 and 9,000 kg ha^?1 residue returning significantly increased yield-scaled emissions of CH₄ and CO₂ by 18.1 and 61.5 %, respectively, compared with the treatment of no residue returning. Moreover, the treatment of NT in combination with 3,000 kg ha^?1 residues had the lowest yield-scaled emissions of CH₄ and CO₂ across tillage and residue treatments. In this way, this study revealed that the combination of NT with 3,000 kg ha^?1 residues was a suitable strategy for optimizing carbon emissions and rice grain yield.     

Radiation utilization efficiency,latent heat flux,and crop growth simulation in irrigated rice during post-flood period in east coast of India

To study the radiation utilization efficiency, latent heat flux, and simulate growth of rice during post-flood period in eastern coast of India, on-farm trial was conducted with three water regimes in main plots (W ₁ = continuous flooding of 5 cm, W ₂ = irrigation after 2 days of water disappearance, and W ₃ = irrigation after 5 days of water disappearance) and five nitrogen levels in subplots (N ₁ = 0 kg N ha^?1, N ₂ = 60 kg N ha^?1, N ₃ = 90 kg N ha^?1, N ₄ = 120 kg N ha^?1, and N ₅ = 150 kg N ha^?1) on a rice cultivar, ‘Lalat’. Average maximum radiation utilization efficiency (RUE) in terms of above ground dry biomass of 2.09 (±0.05), 2.10 (±0.02), and 1.9 (±0.08) g MJ^?1 were computed under W ₁, W ₂, and W ₃, respectively. Nitrogen increased the RUE significantly, mean RUE values were computed as 1.60 (±0.07), 1.78 (±0.02), 2.060 (±0.08), 2.30 (±0.07), and 2.34 (±0.08) g MJ^?1 when the crop was grown with 0, 60, 90, 120, and 150 kg ha^?1 nitrogen, respectively. Midday average latent heat flux (on clear days) varied from 7.4 to 14.9 and 8 to 13.6 MJ m^?2 day^?1 under W ₂ and W ₃ treatments, respectively, at different growth stages of the crop in different seasons. The DSSAT 4.5 model was used to simulate phenology, growth, and yield which predicted fairly well under higher dose of nitrogen (90 kg and above), but the model performance was found to be poor under low-nitrogen dose.     

Agronomic,molecular and antioxidative characterization of red- and purple-pericarp rice (Oryza sativa L.) mutants in Taiwan

Pigmentation of rice grain is controlled by Ra, Rc and Rd genes, and the expressions of these genes differ among red-, purple- and white-pericarp varieties. The present study examined the grain yield and the expression of Ra, Rc and Rd genes in non-waxy white-pericarp rice SA418 and waxy white-pericarp rice SA419 and their respective red-pericarp and purple-pericarp mutants with waxy or non-waxy endosperm. Significant variations in 100-grains weight and grain yield were observed among the tested mutants. The Ra was expressed in purple-pericarp mutants, while the Rc was expressed in red-pericarp mutants. The total phenolics, total flavonoids, total anthocyanins and total proanthocyanidins contents and antioxidant activities in the bran part also differed among the mutants. Non-waxy red-pericarp mutant M-69 had heavier 100-grains weight (2.86 g), contained more total phenolics (49.37 mg g⁻¹ bran dry weight) and produced higher grain yield (6.93 t ha⁻¹) than white-pericarp rices SA418 (2.43 g, 2.89 mg g⁻¹ bran dry weight and 2.80 t ha⁻¹, respectively) and SA419 (2.62 g, 2.20 mg g⁻¹ bran dry weight and 6.73 t ha⁻¹, respectively). Thus, the polished rice grain of M-69 can be used for staple food consumption, and its bran parts are useful for producing health-promoting by-product.     

Effect of emergence time,inter- and intra-specific competition on growth and fecundity of Echinochloa crus-galli in dry-seeded rice

Since 2005, the evolution and spread of herbicide-resistant Echinochloa crus-galli biotypes have posed a serious threat to crop production in the Philippines. A comprehensive knowledge of E. crus-galli ecology and fecundity is fundamental in managing different biotypes of this weed. It was hypothesized that (a) high weed plant density produces more biomass and fertile seeds per unit area, (b) rice interference reduces the biomass and fecundity of the weed, and (c) a delay in weed emergence reduces the soil seed bank. In 2013, experiments were conducted in the wet season (WS) and dry season (DS), to understand the effect of E. crus-galli densities (40 and 80 plants m⁻²) on its growth, survival, and fecundity, with varying emergence times of 2, 15, 30, and 45 d after rice emergence (DARE). Relative to the weed plants grown without rice interference, E. crus-galli growth and seed production was lower in the presence of rice. Percent survival and plant height of E. crus-galli declined in a linear manner in the DS, and declined in a quadratic manner in the WS. Tiller number, inflorescence number, inflorescence biomass, and shoot biomass per plant declined in an exponential manner, with a delay in emergence of each cohort relative to rice. Across rice seeding rate, weed density, and emergence time, there was a linear relationship (y = 110x − 272 in the DS and y = 100x − 220 in the WS) between E. crus-galli shoot biomass and the number of seeds plant⁻¹. Relative to the late-emerging weed cohorts, E. crus-galli seed production (1320–1579 seeds plant⁻¹), 1000-seed weight (2.2–2.9 g), and seed yield (2808–2334 kg ha⁻¹) were higher when seedlings emerged with the crop (2 DARE). None of the seedlings that emerged 45 DARE produced viable seeds. Seed germination of the first two cohorts (2 and 15 DARE) ranged from 84 to 91%. The delay in emergence of E. crus-galli beyond 30 DARE reduced the percentage of germinable and viable seeds, and increased the percentage of non-viable seeds produced plant⁻¹. The results suggest that cultural weed management approaches that delay the emergence of E. crus-galli can reduce weed biomass and seed production, and is thus valuable for preventing seed rain to the seed bank by noxious weed biotypes in the field.     

Chemical composition and in vitro total gas and methane production of forage species from the Mid Rift Valley grasslands of Ethiopia

There is increasing interest in sustainable land use in the tropics to optimize animal production while also reducing methane (CH₄) emissions, but information on nutritive value and CH₄‐emission potential of tropical forage species is limited. Samples of 24 grasses and five other forages were collected during the main rainy season on randomly positioned quadrats in semi‐arid grassland in the Mid Rift Valley of Ethiopia. Samples were pooled by species, analysed for chemical composition and incubated with rumen fluid to determine total gas and CH₄‐emission potentials using a fully automated in vitro gas production apparatus. Organic matter digestibility (OMD) and metabolizable energy (ME) contents were calculated from chemical composition and gas production data. Large variability was observed among forages for all nutritional variables considered. The grasses Eleusine multiflora, Pennisetum stramineum, Dactyloctenium aegyptium, Eragrostis aspera, Cenchrus ciliaris and Eragrostis cilianensis showed relatively high OMD (68–72%) and ME values (9·1–10·2 MJ kg^?1 dry matter). Melinis repens, E. multiflora and the non‐legume forb Zaleya pentandra showed relatively low CH₄ to total gas ratios; these species may have potential for use in low CH₄‐emission forage diets. Acacia tortilis fruits had high content of crude protein and moderate ME values, and may be an ideal feed supplement for the grazing ruminant. Sodium content was below the recommended level for ruminants in all the forage species. Overall, the pasture stand during the main growing season was evaluated as having moderate nutritional quality.