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1.
Independent selection for seed free tryptophan content and vernalization response in chickpea domestication
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Ruth P. van‐Oss Avi Gopher Zohar Kerem Zvi Peleg Simcha Lev‐Yadun Amir Sherman Hong‐Bin Zhang George Vandemark Clare J. Coyne Ofer Reany Shahal Abbo 《Plant Breeding》2018,137(3):290-300
Chickpea shows a distinct domestication trajectory vis‐a‐vis pod dehiscence and growth cycle mediated by vernalization insensitivity compared with its companion Near Eastern legumes. Our objectives were: (i) to map the quantitative trait loci (QTLs) associated with vernalization response and seed free tryptophan in domesticated × wild chickpea progeny and (ii) estimate the genetic correlation between vernalization response and free tryptophan content. A domesticated × wild chickpea cross was used to document phenotypic segregation in both traits and to construct a skeletal genetic map for QTL detection. A number of vernalization response and seed free tryptophan content QTLs were documented in both F2 and F3 generations. No significant genetic correlation between these two traits was observed. Epistatic relationship between two free tryptophan loci was documented. It is evident that selection for high seed tryptophan is easier to accomplish relative to selection for vernalization insensitivity. This suggests that the two traits were selected independently in antiquity, thereby corroborating earlier claims for conscious selection processes associated with chickpea domestication. 相似文献
2.
Potato (Solanum tuberosum L.) is a major food and cash crop mainly grown by small-scale farmers in the highland regions of Uganda. Changing global weather patterns require varieties that are able to grow within the short rainfall cycles and yield optimally under the prevailing conditions. The objectives of this study were to estimate the combining ability effects for early maturity, yield and yield related traits in potato. Eighteen F1 families generated from two sets of 12 parents using a North Carolina Design II were evaluated for days to 50% flowering, leaf senescence, yield and yield related traits in two different locations. Both additive and non-additive genetic effects influenced the expression of traits. However, additive genetic effects were predominant over the non-additive for most of the traits. The GCA/SCA ratios were 0.68 and 0.78 for days to 50% flowering and average tuber weight. Broad sense heritability estimates were 0.70 for total tuber weight and 0.78 for days to 50% flowering. The predominance of additive genetic effects imply that, genetic gains can be achieved through different selection methods and traits transferred to the respective progenies. Parents Rwangume, 396,038.107, 395,011.2, NKRK19.17, 393,077.54, Kimuri, and 392,657.8 had desirable GCA effects for the number of days to flowering and yield related traits. Families of Rwangume?×?NKRK19.17, 393,077.54?×?395,011.2, 396,038.107?×?Rwangume and 396,038.107?×?395,011.2 had desirable SCA effects for yield and number of days to 50% flowering. The selected parents and families will be subjected to further clonal evaluation and selection. 相似文献
3.
To determine the effect of Vrn‐H2 locus on plant developmental and agronomic traits, detailed controlled environment tests involving a factorial set of vernalization and photoperiod treatments were carried out using doubled haploid lines developed from a facultative (Vrn‐H2?) × winter (Vrn‐H2+) barley cross. The allele phase at the Vrn‐H2 locus influenced heading date as well as the developmental and agronomic traits. The performance of Vrn‐H2+ lines was significantly influenced by vernalization: reproductive fitness traits showed significant decreases without vernalization. However, the effects of alleles at the Vrn‐H2 locus extended beyond simple satisfaction of the vernalization requirement. Vrn‐H2+ lines showed increased reproductive fitness compared with Vrn‐H2? lines when vernalization was followed by a long photoperiod. The responses of the two Vrn‐H2 allele classes to photoperiod duration were quite different in terms of heading date, developmental and agronomic traits. These results suggest that alleles at the Vrn‐H2 locus – and/or tightly linked gene(s) – respond primarily to the exogenous signal of vernalization (temperature), but when the vernalization requirement has been fulfilled, they also respond to photoperiod duration. 相似文献
4.
Summary The inheritance of flowering time and its component processes, vernalization and photoperiod response, were studied in two crosses of subterranean clover (Trifolium subterraneum L.) using a field sowing and four controlled environment sowings with different combinations of vernalization and photoperiod. Time to flowering was under polygenic control and was highly heritable. For both vernalization and photoperiod response, there was dominance for a low response, or earliness. A simple genetic control was indicated for photoperiod response. The results for vernalization response were not clear cut, although the character appeared to be under polygenic control. An interaction between vernalization and photoperiod response was evident in three of the four cultivars studied. This made it impossible to separate the effect of these two component processes and complicated the study of their inheritance. Node of first flower on the main stem was closely related to flowering time and its use led to similar conclusions in the inheritance studies. 相似文献
5.
Selecting high yielding genotypes with stable performance is the breeders’ priority but is constrained by genotype × environment (G×E) interaction. We investigated canola yield of 35 genotypes and its stability in multiple environment trials (MET) in south-western Australia and the possibility to breed broadly-adapted high yielding genotypes. The Finlay–Wilkinson (F–W) regression and factor analytic (FA) model were used to investigate the G×E interaction, yield and genotype stability and adaptability. The cross-over response in the F–W regression, substantial genetic variance heterogeneity, and the genetic correlations in the FA model demonstrated substantial G×E interaction for yield. Cluster analysis suggests low, medium and high rainfall mega-environments. F–W regression indicated that genotypes with high stability (e.g. low regression slope values) produced relatively low yield and vice versa, but also identified broadly adapted genotypes with high intercepts and steep regression slopes. The FA model provided a more detailed analysis of performance, dividing genotypes by positive, flat or negative responses to environment. In general, early flowering genotypes responded negatively to favourable environments and vice versa for late flowering genotypes. More importantly, a few early flowering hybrids with long flowering phases were consistently productive in both low and high yielding environments, showing broad adaptability. These productive hybrids were consistent with those identified earlier by high F–W intercept and slope values. Hybrids were higher yielding and more stable than open-pollinated canola, as was Roundup-Ready® canola compared to the three other herbicide tolerance groups (Clearfield®, Triazine tolerant, conventional). We conclude that yield stability and high yield are not mutually exclusive and that breeding for broadly adapted high yielding canola is possible. 相似文献
6.
Murali K. Darapuneni Gaylon D. Morgan Amir M. H. Ibrahim Robert W. Duncan 《Euphytica》2014,195(2):279-285
Vernalization and photoperiodism are two important physiological processes related to yield of many cool-season annual crops. The flowering response of 20 flax (Linum usitatissimum L.) genotypes to two vernalization regimes (vernalized and unvernalized) and two photoperiod treatments (10 and 14 h) was evaluated in a growth chamber study in 2010 and 2011. The results suggest that photoperiod, vernalization, and genotype all had an effect on earliness as measured by days to anthesis. Unlike flax grown in the Upper US Midwest and Canada, Texas flax is grown in the fall due to high spring and summer temperatures. Genotype interaction was observed with both vernalization and photoperiod. Specifically, flax genotypes from Texas (winter type) were sensitive to both vernalization and photoperiods for flowering. Texas genotypes delayed anthesis for 7 days or more in unvernalized seedlings, whereas flowering time of most other spring grown flax genotypes was unaffected by the vernalization treatments. Texas genotypes also delayed anthesis for 12 days or more under vernalized and short day conditions, whereas most other genotypes were not influenced by photoperiodism in vernalized seedlings. The selection for vernalization and photoperiodic sensitivity in Texas genotypes and introgression of these traits into recently adapted spring grown genotypes is needed for development of high yielding flax genotypes for southern Great Plains production areas. 相似文献
7.
8.
Muhammad Iqbal Alireza Navabi Donald F. Salmon Rong-Cai Yang Brenda M. Murdoch Steve S. Moore Dean Spaner 《Euphytica》2007,154(1-2):207-218
Due to the short growing season in the high northern latitudes, the development of early maturing spring wheat (Triticum aestivum L.) cultivars is important to avoid frost damage which can lower production and quality. We investigated earliness of flowering and maturity, and some associated agronomic traits, using a set of randomly selected high northern latitude adapted spring wheat cultivars (differing in maturity) and their F1 and F2 crosses made in a one-way diallel mating design. The parents, and their F1 and F2 crosses were evaluated under field conditions over 2 years. Anthesis and maturity times were controlled by both vernalization response and earliness per se genes, mainly acting additively. Non-additive genetic effects were more important in controlling grain fill duration, grain yield and plant height. Additive × additive epistatic effects were detected for all traits studied except time to anthesis. Segregation analyses of the F2 populations for time to anthesis indicated the presence of different vernalization response genes. Molecular genetic analyses revealed the presence of Vrn-A1 and Vrn-B1 genes in the parental cultivars. Narrow-sense heritability was medium to high (60–86%) for anthesis and maturity times but low to medium (13–55%) for grain fill duration, plant height and grain yield. Selection for early flowering/maturity in early segregating generations would be expected to result in genetic improvement towards earliness in high latitude spring wheats. Incorporation of the vernalization responsive gene Vrn-B1 in combination with vernalization non-responsive gene Vrn-A1 into spring wheats would aid in the development of early maturing cultivars with high grain yield potential for the high latitude wheat growing regions of the northern hemisphere. 相似文献
9.
The extent and distribution of incompatibility between gene pools (Mesoamerican and Andean) and evolutionary classes (wild,
landrace and bred) of the common bean (Phaseolus vulgaris) was explored by seeking anomalous values of highly heritable quantitative traits in the progeny of crosses. Clear incompatibility
(no progeny or sterile or deformed progeny) was shown by 28 crosses in a 12-parent wild × bred diallel cross, and 37 crosses
in a 12-parent landrace × bred diallel cross. Incompatibility was particularly common in the progeny of certain genotypes,
but was not consistently associated with the division between gene pools or evolutionary classes. When crosses showing clear
incompatibility were eliminated from the data, days to flowering, number of seeds per pod, log (weight per seed) and seed
roundness in the F1 generation gave a good fit to an additive-dominance model, confirming that there is no overall tendency to incompatibility
between the gene pools. There was a division between the gene pools with regard to log (weight per seed), as expected, but
there was no such division, with regard either to the means of the parent lines or the distribution of the statistics V
r and W
r (which indicate the distribution of dominant alleles between genotypes), for the other quantitative variables. Differences
between reciprocal crosses were strikingly widespread, and appeared generally to be due to cytoplasmic effects or cytoplasmic
× nuclear interactions rather than maternal effects, indicating that the direction in which a cross is made may have a perceptible
effect on the progeny that can be obtained from it.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
10.
Hybrid performance and heterosis in strawberry (Fragaria × ananassa Duchesne), regarding acidity,soluble solids and dry matter content in fruits
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Elżbieta Kaczmarska Jacek Gawroński Ewa Jabłońska‐Ryś Marta Zalewska‐Korona Wojciech Radzki Aneta Sławińska 《Plant Breeding》2016,135(2):232-238
A top‐cross‐mating design among 29 S4 inbred lines and tester (cultivar ‘Dukat’) was carried out to study their breeding value in terms of general combining ability (GCA). The objectives of this study were to evaluate the acidity, soluble solids and dry matter contents in fruits of progeny F1 in comparison with S4 inbred lines as well as the cultivars (S0); identify strawberry genotypes with high value of GCA for use in cultivar development; and determine mid‐parent heterosis regarding S4 inbred lines and cultivated strawberry. The 2‐year observations showed statistically significant differences between tested genotypes in terms of the studied traits. The highest breeding value based on GCA was estimated for Chandler 123‐5 for soluble solids and dry matter content, and Kent 7‐6 for acidity. Estimated mid‐parent heterosis had positive and negative values. The highest heterosis in terms of extract and dry matter content (26.71% and 17.50%, respectively) occurred in the offspring Chandler 123‐5 × ‘Dukat’, but as regards acidity in hybrid Chandler 123‐22 with cv. ‘Dukat’. The study of genetic divergence by dendrograms may help to identify parents suitable for obtaining hybrids with higher heterosis effects. 相似文献
11.
Gene effects of Cicer reticulatum on qualitative and quantitative traits in the cultivated chickpea
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The gene effects of Cicer reticulatum on both double‐podding as qualitative traits and yield criteria as quantitative traits in interspecific hybridization with cultivated chickpea (Cicer arietinum) have not yet been elucidated, despite the easy acquisition of hybrid progeny between two species. This study sought to answer three questions concerning qualitative and quantitative traits in reciprocal crosses between C. arietinum and C. reticulatum. (i) Is there a similarity in the gene effects of flower colour, pigmentation and double‐podded traits in reciprocal interspecific crosses? (ii) What are the expressivity and penetrance of the double‐podded trait in interspecific crosses? (iii) Which heterosis predicts the occurrence and the extent of transgressive variation? The materials for this study were F1, F2 and F3 progeny derived from a reciprocal cross between C. arietinum and C. reticulatum. As qualitative traits, purple flower colour, pigmentation and single‐podded traits in C. reticulatum were governed by a dominant single gene. Purple flower colour and pigmentation were detected to be linked traits as all progeny had the same phenotypes. As quantitative traits, yield criteria in progeny which were double‐podded had higher values than the single‐podded counterparts. Expressivity and penetrance of the double‐podding trait were superior in progeny derived from C. reticulatum × C. arietinum. The results showed that fruitful heterosis was more useful than residual heterosis in F3 as residual heterosis was mostly negative and fruitful heterosis was suggested in self‐pollinated species such as chickpea that lacks inbreeding depression. Interspecific transgression was significant with respect to chickpea improvement because it represented a potential source of novel genetic variation. 相似文献
12.
Summary The effect of direct vernalization of immature embryos on flowering was studied in six winter wheat genotypes. Fourteen-, 17-, and 20-day-old embryos were excised and vernalized for 0–6 weeks on synthetic medium during a conditioning period. Percent germination of embryos was high (overall 96.1%), and free from genotypic effects. Genotypes differed for flowering in response to cold treatment of excised embryos. Embryo vernalization was as effective as or more than conventional vernalization (control, seedling vernalization for 6 weeks). Seventeen-day-old embryos were the most responsive to vernalization. With a 5-week vernalization of 17-day-old embryos, the percentage of plants anthesed was higher than those from 14-and 20-day-old embryos. For 17-day-old embryos vernalized for 5 weeks, the mean number of days from culture to anthesis was less than that of 6 week vernalization, less than that of 14- and 20-day-old embryos, and less than controls.Purdue Univ., Agronomy Dept., W. Lafayette, IN 47907, USA. 相似文献
13.
Summary Fourteen lines of linseed (Linum usitatissimum L.) were evaluated for general and specific combining ability through line × tester cross analysis using five diverse testers in two different environments. Seventy F1's and nineteen parental genotypes were raised in completely randomized block design with two replications with respect to yield and yield related traits. Considerable genetic variation was observed for all the traits studied. The additive as well as non-additive gene effects played significant role in the inheritance of yield and related traits with preponderance of non-additive gene effects for all the traits studied. Higher proportion of general combining ability × environment interaction variance as compared to specific combining ability × environment estimates was recorded. Additive genetic variances were more sensitive than non-additive genetic variances to the changing environment. Low estimates of heritability (narrow sense) for primary branches per plant, capsules per plant, seeds per capsule, seed yield per plant, harvest index and oil content and medium heritability for days to 50% flowering, days to maturity, plant height and 1000-seed weight was observed. Among the female parents LCK-9816, Parvati, Himalini, KL-168 were good general combiners for yield and related traits along with oil content, whereas among male parents, Surbhi and KL-224 were good general combiners for yield related traits and oil content but KL-221 was good general combiner for seed yield and related traits. 相似文献
14.
Late blight, caused by Phytophthora infestans (Mont.) de Bary, is the most destructive disease of potato worldwide. As this pathogen can rapidly overcome major race‐specific resistance genes, identifying the basis for enhanced quantitative resistance has become a crucial element for implementing advanced breeding strategies. A population of 230 full‐sib progeny derived from a cross between two diploid hybrid Solanum phureja × S. stenotomum clones was evaluated for foliage resistance against late blight in replicated trials at multiple locations in Pennsylvania between 1999 and 2002. In field experiments, plants were evaluated visually for per cent defoliation, and area under the disease progress curve (AUDPC) was determined. The two parents and three control cultivars (‘Atlantic’, ‘Kennebec’ and ‘Katahdin’) were included in all trials. In all three experiments, the presence of a significant number of clones exhibiting transgressive segregation were observed. There were significant differences among environments as well as among clones, and the clone × environment interaction was also significant. Stability analysis revealed that 37 clones made a significant contribution to the overall environment × clone interaction. Broad‐sense heritability for resistance, measured as AUDPC, was estimated as 0.67. The overall results indicate the presence in this potato family of a high level of field resistance against late blight. This segregating diploid family appears to be a good candidate for quantitative trait loci mapping to identify and characterize the genetic components of partial late blight resistance. 相似文献
15.
X. Q. Zhang X. D. Wang P. D. Jiang S. J. Hua H. P. Zhang Y. Dutt 《Plant Breeding》2007,126(4):385-391
The present study was conducted to investigate the relationship between parental molecular marker diversity and hybrid performance in both intra‐ and interspecific hybrids of cotton to evaluate the feasibility of predicting hybrid performance using molecular markers. Three cytoplasmic male sterile (CMS) lines were crossed with 10 restorer lines to produce 22 F1 hybrids during 2003. Of 22 F1s, 14 hybrids were intraspecific (Gossypium hirsutum × G. hirsutum) and eight interspecific (G. hirsutum × G. barbadense). These 22 F1 hybrids and their parents were evaluated for yield and fibre quality traits at Zhejiang University, Hangzhou, China during 2004 and 2005. Genetic distances (GD) among the parents were calculated from 56 random‐amplified polymorphic DNAs (RAPD) and 66 simple sequence repeat (SSR) marker data, and their correlation with hybrid performance and heterosis were analysed. The parents could be discriminated into G. hirsutum and G. barbadense clusters by cluster analysis based on both RAPD and SSR markers data. The correlation (r = 0.503, P ≤ 0.05) was calculated between GDrapd (GD based on RAPD markers) and GDssr (GD based on SSR markers). Correlation of GD with hybrid performance and heterosis differed considerably between intra‐ and interspecific hybrids. The correlation between GD and hybrid performance was non‐significant for most of traits within the hybrids of G. hirsutum species. However, it was significantly and positively correlated for fibre length, fibre strength and elongation in interspecific hybrids. The relationship between GD and heterosis was observed to be positively significant for boll weight within hybrids of G. hirsutum with significant and negative correlations for fibre length and elongation. In conclusion, the power of predicting hybrid performance using molecular markers in cotton is low. But, the relationship between SSR marker heterozygosity and hybrid performance can be used to predict fibre length during interspecific hybrid cotton breeding. 相似文献
16.
Shahal Abbo Ofer Goren Yehoshua Saranga Matthias Langensiepen David J. Bonfil 《Plant Breeding》2013,132(2):200-204
Modifying plant architecture is considered a promising breeding option to enhance crop productivity. Modern chickpea (Cicer arietinum L.) cultivars with either compound (wild‐type) or simple leaf shapes are commercially grown but the relationships between leaf shape and yield are not well understood. In this study, a random sample of ‘Kabuli’ type progeny lines of both leaf types, derived from two crosses between modern American simple leaf cultivars and early‐flowering wild‐type breeding lines, were planted at different sowing densities. Leaf area development and final grain yield in genotypes of the two leaf types responded differently to changes in sowing densities. Compound leaf lines attained higher leaf area indices and higher grain yields at both low and high sowing densities. Yield responses of the simple leaf lines to increasing sowing density were significantly higher compared to compound leaf genotypes in two of three field experiments. The prospects for utilizing the simple leaf trait as a breeding target for short‐season growing areas are discussed. 相似文献
17.
R. Keeve G. H. J. Krüger H. L. Loubser J. A. M. Van Der Mey 《Journal of Agronomy and Crop Science》1999,183(4):217-223
An experiment to determine the effect of temperature and photoperiod on Lupinus albus under controlled environmental conditions was carried out, using the three Lupinus albus genotypes 'Tifwhite', 'Esta' and 'Kiev', and three temperature (10/20, 18/28 and 20 °C continuously) and two photoperiod (8 and 16 h daylength) regimes, in all combinations. Half of the seeds were vernalized for 21 days at 4 °C to alleviate the obligate vernalization requirement of Tifwhite. Although Esta and Kiev do not have obligate vernalization requirements, they were influenced by this vernalization period. Observations included the duration of the period from planting to seedling emergence, the duration of the period from planting to the beginning of flowering and the duration of flowering. The vernalization treatment accelerated plant development in all genotypes. The period from planting to emergence was shorter under the higher temperature regime. For all genotypes, the period from planting to flowering was shorter under the longer photoperiod, the same trend as would be expected for long-day plants. Duration of flowering periods were, in contrast to pre-flowering periods, shorter for all genotypes at cooler temperatures. The results of this study confirm that photoperiod does contribute to the growth period from planting to flowering in L. albus and that this species does behave as a long-day plant. 相似文献
18.
Ginette Séguin‐Swartz Jacqueline A. Nettleton Connie Sauder Suzanne I. Warwick Richard K. Gugel 《Plant Breeding》2013,132(4):390-396
The potential for gene flow between Camelina sativa, a promising edible and industrial oil crop in Canada, and its wild North American relatives C. alyssum, C. microcarpa and C. rumelica subsp. rumelica, was investigated. Sequence analysis of the nuclear ribosomal internal transcribed spacer region (ITS) was used to differentiate Camelina species and/or accession‐specific nucleotide markers and identify interspecific F1 hybrids. ITS analysis identified hybrids in progeny of C. alyssum × C. sativa, C. microcarpa accession 36010 × C. sativa and C. sativa × C. rumelica subsp. rumelica. Seed set on C. alyssum × C. sativa F1 and F2 progeny was similar to the parents; few seeds were produced on hybrid progeny of C. microcarpa accession 36010 × C. sativa and C. sativa × C. rumelica subsp. rumelica. The study provided evidence that should the species have sympatric distributions and overlapping flowering periods, gene flow between C. sativa and its wild North American relatives is possible and that it would most likely occur with C. alyssum given the high fertility of the F1 hybrids recovered. 相似文献
19.
P. Krajewski J. Bocianowski M. Gawłowska Z. Kaczmarek T. Pniewski W. Święcicki B. Wolko 《Euphytica》2012,183(3):323-336
Quantitative trait loci for yield, yield components and seed protein content were investigated on the basis of experiments
performed with two populations of pea (Pisum sativum L.) lines derived from linked crosses between lines Wt11238, Wt3557 and Wt10245 with contrasting characteristics. The yield-related
traits were defined as components giving the grain yield in a multiplicative way. The aim was to clarify the genetic architecture
of the relation between seed yield, its components and protein content, with a possible inclusion of the role of epistasis
in this explanation. To take full advantage of the availability of the two populations, additive QTL effects and both types
of epistasis were analysed: the QTL by genetic background interaction and the first-order QTL–QTL interaction. The two hybrid
populations differed with respect to the prevailing gene action, which in the Wt11238 × Wt3557 progeny was mainly additive,
while in the Wt10245 × Wt11238 progeny mainly epistatic. Some loci with previously reported, large, repeatable, but contradictory
effects on yield and protein content were confirmed. New loci with alleles coming from the protein-rich Wt11238 line, positive
for yield components, were identified. It was found that the first order QTL–QTL interaction events were more frequent for
the loci showing the QTL by genetic background interaction. 相似文献
20.
Francis Kwame Padi 《Euphytica》2007,158(1-2):11-25
Twenty-four cowpea genotypes were evaluated under sole cropping or additive series intercropping with sorghum from 2004 to
2005 at four sites representative of the Guinea and Sudan savannah ecologies in Ghana. The aim was to determine whether cowpea
breeding programs that emphasize selection under sole-crop conditions have the potential to produce cultivars that are effective
under additive series intercropping. Genotype × cropping systems interaction was significant for days to 50% flowering but
not for grain yield, biomass and other studied traits. Genotypic yield reaction to cropping systems indicated that bridging
the yield gap between sole cropping and intercropping systems is best addressed by agronomic interventions that reduce stress
on intercrop cowpea rather than by selecting for specifically adapted genotypes for intercropping. Significant genotype × environment
interactions were observed for all traits when data was pooled over cropping systems. Partitioning of the genotype × environment
interaction variance indicated that days to 50% flowering was dominated by heterogeneity of genotypic variance, whereas genotype × environment
interactions for grain yield and biomass was mainly due to imperfect correlations. Large differences in genotypic yield stability
were observed as estimated by the among-environment variance, regression of yield on the environmental index, Kataoka’s index,
and by partitioning of genotype × environment interaction sum of squares into components attributable to each genotype. The
results suggest that in regions where genotype × environment interaction for yield frequently causes re-ranking across environments,
genotypes with the least contribution to the interaction sum of squares are likely to be most productive. On the whole, the
results support the contention that breeding under sole-crop conditions has the potential to produce cultivars effective under
intercropping conditions. 相似文献