An outbreak of sheep pox associated with goat poxvirus in Gansu province of China

Three strains of Capripoxviruses (CaPVs) were isolated from an outbreak of sheep pox in Gansu province of China. They were analyzed by P32 gene-based molecular methods and a species-specific PCR based on the RPO30 gene. Two bands which are specific to goat poxvirus (GTPV) were observed after the PCR products of P32 gene were digested with the endonuclease of Hinf I. Moreover, an amplicon of 172 bp, which is specific to GTPV, was amplified from the viruses by using the RPO30 gene-based PCR. Sequence analysis of the P32 genes showed that three nucleotide bases for coding residue of aspartic acid which are located at 163-165 position of P32 gene of sheep poxvirus (SPPV) were absent, and six single nucleotide substitutions which are characteristic of GTPV were present. The viruses were genetically closer to GTPV strains and clustered into the GTPV branch of the phylogenetic tree constructed on the basis of the P32 gene. The results characterized the isolated viruses as GTPV. It is the first report of an outbreak of sheep pox associated with GTPV in China.     

绵羊痘的诊断与防治

2003年11月下旬,秦安县叶堡乡武装村有一农户从外地购进小尾寒羊数只,与其它羊只混合,采取舍饲与放牧结合方式饲养,12月初,发现部分羊只精神不振,采食量减少,眼结膜潮红,鼻孔流出浆液或脓性分泌物,随后在皮肤无毛或少毛处出现红斑,继而形成丘疹凸出皮肤表面,2只体弱羊死亡。数日后,本村另外2户农户家的绵羊也相继发病,3户农户共饲养绵羊97只,发病39只,发病率40．2％,死亡8只,死亡率20．5％。     

绵羊痘的防制

绵羊痘是由山羊痘病毒属绵羊痘病毒引起的以皮肤和粘膜发生痘疹为主要症状的一(新疆天康畜牧生物科技股份有限公司生产)0.5mL/只,可有效预防本病的发生。对发病羊群中尚未发病或邻近受威胁的羊只进行紧急免疫,也可起到一定的预防作用。5.2隔离封锁扑杀绵羊痘发病率高,病死率高,     

绵羊痘病毒的分离与鉴定

利用羔羊睾丸细胞、Marc-145、Vero细胞对病毒进行分离和培养,通过动物回归试验、组织病理学切片的观察、透射电镜观察、PCR鉴定等方法进行病毒的鉴定,并参照GenBank中绵羊痘病毒P32基因序列设计并合成1对特异性引物,成功地对分离株的P32基因进行克隆、测序,并与多株参考毒株进行同源性比对分析。结果显示:透射电镜下观察到典型的痘病毒粒子;动物回归试验中试验动物临床症状为典型的羊痘症状;组织病理学切片中观察到羊痘病毒嗜酸性包涵体;通过DNASTAR与参考毒株进行同源性比对,与参考株同源性为97.2%~99.5%。结果表明:该分离株为绵羊痘病毒,并命名为SPPV-NeiMengG2015。     

Factors associated with productivity in Canadian sheep flocks.

A mail survey of Canadian sheep flocks registered on the Record of Performance program was conducted, and the association of management practices and diseases with productivity was studied using multiple regression techniques. The relationships between management practices and diseases of lambs which were associated with production were also investigated using discriminant analysis. Flocks in the maritimes had a lower average flock productivity than those in Ontario-Quebec and the western provinces. In purebred flocks, the average adjusted weight at 100 days of age was higher than in other types of flocks, and the prevalence of scours was lower. At the herd level, the rates of pregnancy toxemia and vaginal prolapse were associated with heavier lambs, while the average daily weight gain between 50 and 100 days of age was lower in herds affected by scours and starvation. Using pasture and bush as winter housing was also detrimental to lamb productivity. No management practices discriminated between case and control flocks for scours and starvation in lambs. It was concluded that the reasons for the geographic differences should be investigated as should the management differences between purebred and other producers. Prevention of scours and starvation in lambs should be given priority in order to increase productivity.     

鸡痘鹌鹑化弱毒细胞苗接毒培养工艺探索

在鸡胚成纤维细胞(CEF)培养鸡痘鹌鹑化弱毒细胞苗的工艺中,采用同步接毒工艺,通过调整单位细胞浓度和单位病毒含量,以细胞单层形成时间和收获时间分别为24 h和96 h为标准,确定细胞浓度和接毒浓度分别为1.2×106 ～1.5×106个/mL和102 ～103 EID50/mL较为合适.     

治奶牛气臌

20 0 2年 7月 1 0日 ,本乡荣生、长安及蓬生三个村绵羊 80 0余只相继发生痘病 ,死亡90余只 ,死亡率高达 1 2 % ,现将诊疗及防治情况报告如下。1　临诊症状病羊精神沉郁 ,食欲废绝 ,体温升高 ,结膜潮红 ,有浆性、粘性或脓性鼻液 ,呼吸和脉搏增速 ,经 1～ 4ｄ发痘。先在皮肤无毛或少毛部位如眼周围、唇、鼻、四肢和尾内侧出现红斑 ,1～ 2ｄ后形成淡红色或灰白色丘疹 ,突出皮肤表面 ,呈结节状。结节在几天之内变成水疱 ,水疱内容物起初象淋巴液 ,后变成脓性 ,如果无继发感染则在几天内干燥成棕色痂块。痂块脱落留一个红斑 ,以后颜色逐渐变淡而…     

绵羊痘的诊疗及防治

2002年7月10日,本乡荣生、长安及蓬生三个村绵羊800余只相继发生痘病,死亡90余只,死亡率高达12%,现将诊疗及防治情况报告如下.