牛传染性鼻气管炎病毒的分离和鉴定

结荷兰进口的,临诊上表现牛传染性鼻气管炎(IBR)症状的奶牛的22份眼、鼻分泌物作 IBR 病毒分离,获得了4株病毒。通过血清学、病毒学、理化特性、病毒核酸型和电镜观察证实,分离到的病毒均为牛传染性鼻气管炎病毒。     

牛传染性鼻气管炎检测方法的研究进展

牛传染性鼻气管炎病毒(IBRV)是引起牛传染性鼻气管炎的病原。文中介绍了牛传染性鼻气管炎的各种检测方法的研究进展,包括病理组织学诊断、病毒的分离鉴定、免疫学检测和核酸分子检测,为牛传染性鼻气管炎的检测提供了有价值的参考。     

奶牛传染性鼻气管炎病毒gG基因PCR检测方法的建立

本试验旨在建立一种PCR技术,既能快速检测牛传染性鼻气管炎病毒,又能区分同属病毒牛疱疹病毒5型和伪狂犬病病毒。根据基因库中牛传染性鼻气管炎病毒gG基因的特异性引物,建立PCR方法,对牛传染性鼻气管炎病毒参考毒株和阳性样本进行扩增,结果均能扩增出一条463 bp的特异性条带;对同属的牛疱疹病毒5型进行扩增,获得651 bp和431 bp两条带;对同属伪狂犬病病毒进行扩增,获得493 bp的条带;而非相关病毒(如猪呼吸与繁殖综合征病毒等),不能扩增出条带。对牛传染性鼻气管炎病毒的检测灵敏度为2×10^-3 PFU/mL。鉴于该方法具有良好的灵敏度和特异性,将在牛疱疹病毒感染诊断和标记疫苗免疫后的鉴别诊断方面具有良好的应用前景。     

IBRV DQ分离株gI基因的克隆与序列分析

通过PCR扩增得到牛传染性鼻气管炎病毒大庆分离株(IBRV-DQ)gI基因上一个398 bp的片段.扩增产物经克隆、测序,所得到的核苷酸序列与GenBank上已发表的此病毒其他分离株的核甘酸序列相比,其同源性在95.5%～99.3%,表明IBRV gI基因非常保守.在系统进化树中IBRV-DQ株与U14106.1、U14107.1株亲缘关系较近,属于同一个分支.     

牛传染性鼻气管炎诊断方法的研究进展

牛传染性鼻气管炎是由牛传染性鼻气管炎病毒引起的一种牛的热性、急性、接触性传染病。该病是世界动物卫生组织(OIE)规定的必须上报的疾病之一,在我国也被列为二类疫病。牛传染性鼻气管炎可降低牛的肥育率、繁殖率和产奶量,给养牛业造成了重大经济损失。从病毒的分离鉴定、血清学以及分子生物学等方面对该病的诊断方法进行综述,以期为牛传染性鼻气管炎的检测和防控提供参考。     

牛传染性鼻气管炎诊断方法研究进展

牛传染性鼻气管炎是由牛传染性鼻气管炎病毒引起的一种牛的热性、急性、接触性传染病。该病是世界动物卫生组织(OIE)规定的必须上报的疾病之一,在我国也被列为二类疫病。牛传染性鼻气管炎可降低牛的肥育率、繁殖率和产奶量,给养牛业造成了重大经济损失。从病毒的分离鉴定、血清学以及分子生物学等方面对该病的诊断方法进行综述,以期为牛传染性鼻气管炎的检测和防控提供参考。     

牛传染性鼻气管炎病毒的分离鉴定与灭活疫苗免疫效果研究

由2例疑似牛传染性鼻气管炎（IBR）病例的荷斯坦奶牛分离到一株病毒,命名为IBRV—C1株。该病毒可被IBR标准阳性血清完全中和;接种MDBK细胞可出现IBR病毒典型细胞病变效应;选取IBR病毒gB蛋白基因序列设计引物进行PCR检测和基因测序,结果可扩增出特异性目的片段;动物回归试验显示,3头牛均可见体温升高、鼻流粘液、呼吸困难等典型的IBR临床症状。在此基础上制备了三批牛传染性鼻气管炎灭活疫苗,并进行了疫苗安全性和效力试验,结果表明三批疫苗对靶动物安全,免疫效果较好,免疫牛中和抗体效价几何平均值可达1：41以上,攻毒保护率达5／5。     

牛传染性鼻气管炎(综述)

牛传染性鼻气管炎(Infectious Bovine Rhinotracheitis)是由病毒引起的牛的一种高度传染性疾病。又名传染性牛坏死性鼻气管炎、坏死性鼻炎、“红鼻子”病、牛交媾疹、传染性脓疱外阴道炎。1956年在美国科罗拉多州的育肥牛群中第一次发现,并为Madin氏等分离到牛传染性鼻气管炎病毒。通常表现为鼻气管炎、结膜炎、脑膜炎、流产,并引起母牛传染性脓疱外阴道炎和公牛的龟头包皮炎。病程短(7—10天),大部份能康复。但仍能成为潜在的带毒者。     

牛传染性鼻气管炎的防治

牛传染性鼻气管炎是由牛传染性鼻气管炎病毒引起的急性接触性传染病,又称牛疱疹病毒Ⅰ型感染、红鼻病或牛传染性坏死性鼻炎.患牛临床特征表现为呼吸困难和发热,有鼻炎、鼻窦炎、喉炎和气管炎.世界动物卫生组织(OIE)将牛传染性鼻气管炎列为B类疫病. 1 病原 牛传染性鼻气管炎病毒属于疱疹病毒,病毒呈球状,直径约200毫微米.可在牛肾、牛胎肾、猪肾、羊肾及马肾细胞上生长,并可发生细胞病变,均产生核内包涵体.     

牛传染性鼻气管炎

牛传染性鼻气管炎是由病毒引起的一种高度传染性疾病。又名牛传染性坏死性鼻气管炎、坏死性鼻炎、“红鼻子”病、牛交媾疹、传染性脓疱外阴道炎。1956年在美国科罗拉多州的育肥牛群中首次发现,并为Madin氏等分离到病毒。通常表现为鼻气管炎、结膜炎、脑膜炎、流产、母牛脓疱外阴道炎和公牛的龟头包皮炎。病程短(7—10天),病畜大多数     

内蒙古地区部分奶牛场牛传染性鼻气管炎的血清学调查

为了解内蒙古地区牛传染性鼻气管炎在奶牛场的流行情况,对内蒙古地区的16个大、中、小型奶牛养殖场的2 321头奶牛,应用ELISA方法进行牛传染性鼻气管炎的血清流行病学调查。结果显示,5个大型奶牛场牛传染性鼻气管炎的阳性率在68.5%~100%之间,5个中型奶牛场的阳性率在34.0%~84.2%之间,6个小型奶牛场的阳性率在32.6%~89.3%之间,平均阳性率为75.7%。     

Isolement du virus de la rhino-trachéite infectieuse bovine à partir du liquide synovial du tarse d''un taureau

Isolation of infectious bovine rhinotracheitis virus from the tarsal joint of a bullInfectious bovine rhinotracheitis virus was isolated from the right tarsal joint of a bull who was showing signs of a systemic viral infection. The clinical signs manifested by 16 bulls of this herd are described, the laboratory methods used are listed and the results are analysed and discussed.     

Effect of maternal antibody upon vaccination with infectious bovine rhinotracheitis and bovine virus diarrhea vaccines.

This report presents the normal rate of decay of maternal antibody and the influence of maternal antibody on responses to a single vaccination with modified-live bovine virus diarrhea and infectious bovine rhinotracheitis virus vaccines at 196 days of age and on response to vaccinations with the same vaccines given twice at 84 and 196 days of age. Passive immunity decreased to near zero over the first six months of life for both bovine virus diarrhea and infectious bovine rhinotracheitis controls. All calves seroconverted to bovine virus diarrhea vaccine at 84 days of age, even though high levels (greater than 1:32) of maternal antibodies were present. These calves did not seroconvert to infectious bovine rhinotracheitis vaccine at 84 days of age when high levels (less than 1:16) of maternal antibodies were present. Calves responded well to bovine virus diarrhea and infectious bovine rhinotracheitis vaccines given only once at 196 days of age after passive immunity disappeared. Calves which were revaccinated with infectious bovine rhinotracheitis seroconverted showing a more rapid response than the single vaccinates. Those revaccinated with bovine virus diarrhea showed an immediate response of small magnitude.     

The association between serological titers in infectious bovine rhinotracheitis virus, bovine virus diarrhea virus, parainfluenza-3 virus, respiratory syncytial virus and treatment for respiratory disease in Ontario feedlot calves.

A seroepidemiological study of the association between antibody titers to infectious bovine rhinotracheitis, parainfluenza-3, bovine virus diarrhea and bovine respiratory syncytial viruses, and treatment for bovine respiratory disease was conducted. A total of 322 calves from five different groups were bled on arrival, then one month later all cases (cattle treated for bovine respiratory disease) were rebled together with an equal number of controls (cattle not treated for any disease). Titers to these viruses varied significantly from group to group. Based on seroconversion, infectious bovine rhinotracheitis virus was active in 4.4%, bovine virus diarrhea virus in 24%, parainfluenza-3 virus in 69.5% and bovine respiratory syncytial virus in 71.3% of the cattle. Cattle with low titers to infectious bovine rhinotracheitis and/or bovine respiratory syncytial viruses on arrival, were at increased risk of subsequent treatment for bovine respiratory disease. Treated cattle also had significantly greater increases to parainfluenza-3 and/or bovine virus diarrhea viruses than control calves. Treatment rates varied considerably from group to group and were not strongly correlated with weight gain in the postarrival period.     

四川部分地区牦牛传染性鼻气管炎血清学检测报告

[目的]为了解四川省牦牛传染性鼻气管炎感染的流行情况。[方法]应用酶联免疫吸附试验对在2012年间采自对四川省甘孜藏族自治州和红原县共377份牦牛血清进行牛传染性鼻气管炎病毒抗体检测。[结果]检出阳性血清样品共105份,阳性率为27.9%。[结论]表明四川省牦牛群中均存在牛传染性鼻气管炎病毒的感染。该研究为该地区牦牛传染性鼻气管防治提供依据。     

牛传染性鼻气管炎诊断和防治

牛传染性鼻气管炎又被称为坏死性皮鼻炎,是由牛传染性鼻气管炎病毒感染引发的一种急性呼吸道疾病。不同年龄的牛感染传染性鼻气管炎病毒后表现的临床症状存在一定差异,年龄较小的牛会表现为明显的临床症状,年龄较大的牛呈现隐性感染或持续性感染,患病牛长时间或终生携带病毒,污染周围环境后造成病情反复流行,病情难以控制、难以消灭。牛传染性鼻气管炎造成的死亡率相对较低,但是会严重影响牛群正常生长与采食,需要掌握该类传染性疾病的流行特点,并进行严格诊断,然后构建有效的防控措施,降低发病率。该文分析牛传染性鼻气管炎的流行病学、临床症状、病理变化、诊断方法和防治措施。     

Effects of swab materials on infectious bovine rhinotracheitis virus.

The recovery rates of infectious bovine rhinotracheitis virus from swab materials were compared. The adsorptive and elutive properties of cotton, polyester, and calcium alginate wool were examined by direct exposure of infectious bovine rhinotracheitis virus to swab materials in buffered tissue culture medium. Calcium alginate wool was virucidal; this was apparent after 2 hours' exposure. Cotton and polyester swab materials exhibited little virucidal effects. The addition of wooden applicator sticks with the swab materials reduced viral titers further.     

Eradication of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) from a herd of beef cattle

Infectious bovine rhinotracheitis virus was eradicated from a 150 cow beef herd at the Animal Diseases Research Institute, Lethbridge, Alberta. Tests used to accomplish this included standard and modified serum-virus neutralization tests and an enzymelinked immunosorbent assay. These results and those of preliminary pilot studies in the herd and in a nonvaccinated, infectious bovine rhinotracheitis-infected 450 cow beef herd suggest that eradication of infectious bovine rhinotracheitis infection can be considered as a practical control alternative to vaccination, and that young animals in purebred herds could be monitored serologically and isolated, to enhance their eligibility for entry into artificial insemination studs or for export.