黑荆树组织培养繁殖技术的研究

通过黑荆树组织培养,探索基本培养基、生长调节物质、蔗糖含量、生长素种类和浓度不同对黑荆树嫩梢增殖、生根的影响。研究结果:采用春季培育42天的实生苗茎段作为外植体,在含有一定成分配比的培养基中,经7-20天,外植体长出1-3个芽梢。在低无机盐分的培养基中附加生长素NAA0.2-0.4mg/1,20-30天即可生根。研究指出:芽丛分化与细胞激动素,无机盐分含量,蔗糖浓度和生长素种类及浓度关系密切。     

曼地亚红豆杉的组织培养快繁技术

以曼地亚红豆杉扦插苗当年生枝的带芽茎段为外植体,进行腋芽增殖再生植株育苗试验.结果表明:MS 6-BA0.05 mg·L-1 NAA 0.5 mg·L-1是曼地亚红豆杉腋芽增殖启动培养的适宜培养基,启动率89.55%.6-BA对芽的启动是必需,但浓度稍高启动率便极显著下降.生长调节物质对芽的启动有利但抑制芽的生长,不添加生长调节物质的MS(有机物加倍)培养基是曼地亚红豆杉侧芽继代培养的适宜培养基,有效芽率为72.73%,平均芽长3.3cm.曼地亚红豆杉组培生根不定根的诱导比较困难,生根率最高27.78%.不定根的发生需要在植物体内建立一个最佳的细胞分裂素和生长素的平衡,而不仅是需要生长素.用IBA 1 000 mg·L-1作生根促进剂处理30 s进行瓶外生根,生根率达到58.33%,是进行曼地亚红豆杉组培无根苗生根诱导的适宜处理方式.     

彩叶朱蕉组织培养育苗技术研究

以彩叶朱蕉优株顶芽和茎段为外植体,设计不同浓度细胞分裂素、生长素以及培养基组合,诱导芽分化、增殖和生根.MS+6-BA 3.0 mg/L+NAA 0.01 mg/L+蔗糖30g/L培养基适用于诱导芽的形成,3/4MS+6-BA 2.0 mg/L+NAA 0.01 mg/L+蔗糖30g/L培养基适用于芽的增殖,3/4MS...     

扁桃茎尖组织培养的研究

为快速繁殖扁桃,以从美国引种的5个品种为试材,从取材、材料的处理、外植体的诱导分化、芽的增殖、生根以及影响试管苗形成的重要因子等方面探讨了扁桃组织培养的技术和方法。经研究得出生长素与细胞分裂素对芽的诱导分化及芽的增殖最佳配比浓度为：在改良MS基本培养基上添加0.5mg/L的NAA,0.5mg/L的KT,0.1mg/L的BA,此配比浓度产生的扁桃有效苗最多;而添加1.5～2.0mg/L的IBA生长素有利于扁桃组培苗生根。     

霍山石斛组培丛生芽诱导增殖及生根技术

以霍山石斛无根试管苗为试材,研究了丛生芽诱导增殖和生根技术.丛生芽诱导增殖通过4因素3水平正交试验,筛选出的最适培养基为1/2MS 0.2 mg/LKT 0.2 mg/L NAA 0.3 mg/L 6-BA.4个因子对霍山石斛丛生芽诱导的影响顺序依次为:KT＞基本培养基＞6-BA＞NAA,其中,KT对霍山石斛丛生芽的诱导效果极为显著.添加不同浓度的生长素及香蕉泥进行生根诱导,其生根效果排序为:香蕉泥＞IBA＞NAA,最佳的生根培养基是MS基本培养基添加20%香蕉泥,生根率可达94.3%.     

非洲菊试管苗生根培养试验

非洲菊试管苗继代培养基中高质量浓度的细胞分裂素可抑制根的诱导；生根培养基中适量的生长素能促进根原基的分化和生长，蔗糖和无机盐质量浓度也影响生根的效果。以MS BZ1.0mg/L IAA0.1mg/L培养基上增殖的芽在1/2MS IBA0.01mg/L上培养，供试品种生根率在15d内达均到100%，且幼苗生长健壮，移出试管后经过适当的锻炼，成活率达到100%。以市售白糖和自来水代替蔗糖和去离子水，以河沙代替琼脂试验均取得了良好的效果。     

濒危植物峨眉拟单性木兰茎段腋芽及愈伤组织诱导

以峨眉拟单性木兰带芽茎段为外植体,MS、WPM、B5为基本培养基,添加不同浓度的细胞分裂素(6-BA)和生长素(IBA、NAA、2,4-D),以不同浓度组合进行腋芽和愈伤组织的诱导.结果表明,基本培养基的类型是腋芽诱导的主要影响因素,以高盐浓度的培养基B5效果较好,MS次之,细胞分裂素的浓度与生长素的比值为15∶1时较适合腋芽的诱导,比值调整为5∶1时有利于茎的伸长生长;愈伤组织的诱导中6-BA起主导作用,其次是2,4-D,再者是基本培养基和NAA,其中2,4-D浓度过高不利于愈伤组织的诱导.     

蓝莓离体快繁及生根特点研究

为了探索蓝莓离体繁育技术方案及其生根的特点,以蓝莓‘夏普蓝’春梢茎段为外植体,就其初代诱导、增殖及生根培养方案进行了筛选实验,对其生根时新梢基部愈伤组织进行了切片观察,并就生根期第0、2、4、6天幼芽中的内源激素IAA及ZR浓度进行了测定。实验结果表明:最适诱导培养基为CQWL+3 mg·L-1 ZT,以此培养基诱导的萌发率达45.2%;最佳增殖培养基为CQWL+1.5 mg·L-1 ZT,增殖系数达4.2倍以上,且增殖的不定芽长势健壮;最佳生根培养基为1/2 CQWL+0.05 mg·L-1 IBA,以此培养基培养的单株长有4~5条根,生根率达73%。切片观察结果表明:生根幼苗基部愈伤组织细胞小,胞质含量多。测定结果表明:添加了0.05 mg·L-1 IBA处理的新梢在生根期间内源激素IAA的浓度升高但变化不明显;内源激素ZR的浓度先下降再升高;IAA/ZR比值稳定。文中认为,培养基CQWL可适用于蓝莓的组织培养;细胞分裂素ZT能促进蓝莓的诱导与增殖;生长素IBA能促进蓝莓的离体生根。     

生长调节剂对自由人槭'冷俊'组培过程中增殖及生根的效应

针对自由人槭组织培养过程中的增殖和生根困难的重要问题，以自由人槭‘冷俊’为试材，运用正交试验研究了生长调节剂对其组培过程中增殖和生根的影响。结果表明：细胞分裂素TDZ对无菌苗增殖效果显著，在MS培养基中添加0．02mg／L的TDZ可有效促进增殖；生长素NAA对无菌苗的生根效果显著，在MS培养基中添加0．1mg／LNAA生根率最高。     

大花、重瓣型非洲紫罗兰组织培养技术研究

对大花、重瓣型非洲紫罗兰(Saintpaulia ionantha)的组织培养研究表明,外植体消毒以0.1%的升汞溶液浸泡5~6 min为宜;不同激素浓度对其愈伤组织诱导、愈伤组织分化、芽丛形成及根的形成有不同的影响;生长素与细胞分裂素的比例决定着愈伤及芽丛的形成;小苗在1/2MS培养基上生根最好。各培养阶段的最适培养基分别为:愈伤组织诱导MS+6-BA1.0 mg/L+NAA 0.5mg/L,芽分化及增殖培养MS+6-BA 2.0 mg/L+NAA0.5 mg/L,生根培养1/2MS+NAA0.1 mg/L或1/2MS+IBA0.1 mg/L。     

Hormone level analysis on adventitious root formation in Eucalyptus globulus

Eucalyptus globulus is the one of the most economically important trees for pulp and paper industries due to its fast growth and short harvesting cycle. However, E. globulus is well known as a woody plant that is hard to propagate vegetatively. In this study, we found wide distribution of rooting ability among seven independent genotypes we tested. Analysis of the mechanism of adventitious rooting by using the auxin transport inhibitor, N-naphthylphthalamic acid, revealed that polar auxin transport is crucial in root formation. The gravitropism of the stem was highly correlated to the percentage of adventitious root formation. Hormonal analysis showed that the levels of indole-3-acetic acid (IAA) were the same between difficult-to-root and easy-to-root genotypes, whereas levels of IAAsp (indole-3-acetyl aspartic acid) were higher in the difficult-to-root genotype, indicating that IAA metabolism might play an important role in adventitious root formation in this species. Levels of several cytokinins exhibited differences between genotypes that varied in their cutting performance and rooting ability. Furthermore, higher level of cytokinin in elongating shoots were correlated with rooting percentage. Taken together, our results indicate that both auxins and cytokinins play a role in adventitious root formation in E. globulus, and that a complex interplay between the levels of auxins and cytokinins and their metabolism might result in root formation in this commercially important plant.     

中华矮樱桃试管苗快速繁殖技术研究

取中华矮樱桃茎段作外植体 ,探讨了其快速繁殖技术 ,筛选出最佳增殖、生根培养基 ,找出了影响试管苗移栽成活率的因素。试管苗增殖系数达到 10 ,生根率 90 7%,移栽成活率 90 %以上。     

风箱树组织培养研究

以风箱树(Cephalanthus occidentalis L.)带腋芽的茎段为外植体,探讨不同植物激素(6-BA、IBA、NAA)对试管苗增殖以及植株再生的影响,建立了一套风箱树离体培养技术体系。试验结果表明:启动最适培养基为MS0;增殖最适培养基为MS+6-BA 1.5mg/L+IBA 0.2mg/L;生根最适培养基为1/4MS0;诱导率可达73.3%,增殖系数可达3.55,生根率89.47%,移栽成活率可达到100%。     

榛子无性繁殖及研究进展

对近几年的榛子压条育苗、硬枝扦插、嫩枝扦插等无性繁殖方法及研究进展作了综述,总结了各种无性繁殖方法中影响生根的因素,不同榛子品种无性繁殖适合不同的生长素和浓度处理。     

阿月浑子组织培养研究

为探索优良经济树种阿月浑子的繁殖方法,以种子胚为外植体,进行了阿月浑子组织培养研究,筛选出了较适宜的增殖和生根培养基。将ＤＫＷ培养基应用于阿月浑子的组织培养,选出了合适的芽增殖培养条件。在诱导生根试验中,根皮苷具有辅助生根作用,使生根率达８０％以上,并移栽成苗。     

In Vitro propagation of juvenileNothofagus leoni Espinosa (Fagaceae)

Nothofagus leoni has a restricted distribution in Chilean forests. This work determines suitable culture conditions forin vitro multiplication and rooting through shoots obtained from seedlings. Broadleaved Tree Medium was suitable for shoot multiplication. A medium with a pulse of 0.55 μM BA in the first subculture and two subcultures on BA-free medium resulted in a multiplication rate at day 63 of × 5.7, without callus growth or shoot neoformation. Rooted shoots of good quality (number and length of roots without callus growth) were obtained with 1.23 μM IBA (91.4% of rooting). The first roots appeared at day 11, reaching a higher speed of rooting at day 15.     

Ecballium elaterium: an in vitro source of cucurbitacins

Biomass and secondary metabolite accumulation were assayed on a wide range of auxins, cytokinins and their combinations using Ecballium elaterium callus tissue. The best combination was that consisting of alpha-naphthalene acetic acid (NAA) and benzylamino purine (BAP) (1 mg/l of medium, each). NAA and BAP were set up in a grid to determine concentrations that produced the best callus as regards fresh and dry weights and yield of cucurbitacins, particularly cucurbitacin E. The best combination for callus proliferation consisted of 5 mg/l NAA and 5 mg/l BAP, reaching a peak at week 5. For cucurbitacin production, an optimum yield was obtained at week 3 with 5 mg/l NAA.     

软枣猕猴桃组织培养的研究

以软枣猕猴桃腋芽为材料,研究了扩繁增殖和生根的最佳条件。结果表明,增殖培养基以MS 6-BA0.5mg/L ZT 1mg/L为好,增殖倍数可达4.00倍;生根培养基以MS IBA 0.2mg/L较好,生根率达87.0%。     

樟树组织培养快繁育苗技术研究

文章报道了应用组织培养育苗技术对樟树家系进行快速繁殖的初步结果.试验表明:带腋芽的茎段外植体在添加细胞分裂素和生长素的MS培养基上可分化形成不定芽,但芽增殖和继代培养过程并不需要添加生长素.在DCR BA 3.0 mg/L的培养基上增殖培养时,平均每个外植体增殖4.7个不定芽,高度小于1.5 cm的不定芽的比例为74.1%;而在DCR BA 4.0 mg/L的培养基上培养时,平均每个外植体增殖2.4个不定芽,高度大于1.5 cm的枝芽比例可提高至58.1%.枝芽接种在DCR IBA 1.5 mg/L的培养基上诱导生根培养28 d的生根率为98%,移植的再生植株95%以上存活.     

黑荆树茎尖培养技术的研究

以黑荆树无菌种子苗的茎类为材料，研究了用不同的细胞分裂素，生长的浓度及其配比，培养基成分的浓度，对茎尖诱导分化芽，芽的伸长及生根的影响，研究结果；培养基为ＭＳ＋６－ＢＡ６．０ｍｇ／Ｌ＋ＬＢＡ０．０１ｍｇ／Ｌ＋ＧＡ３０．１ｍｇ／Ｌ有利于促进茎尖丛生茎的形成和伸长，１／２ＭＳ＋ＮＡＡ０．６ｍｇ／Ｌ有利于诱导芽生根，试管苗移栽成活率达９０％，并在温室里生长正常。