Effect of cuticle removal on the water vapour conductance of egg shells of several species of domestic bird

1. The effect of egg shell cuticle removal, using a 14.7 mM solution of sodium hypochlorite, on the water vapour conductance of egg shells was studied in 5 species of domestic bird.

2. The water vapour conductance of fowl and Muscovy duck egg shells was not affected by the treatment.

3. Cuticle removal increased the conductance of turkey and goose shells, particularly those already relatively porous to water vapour.

4. Removal of cuticles from domestic duck egg shells increased water vapour conductance. In this case the effect was most marked for eggs with lower initial conductances.

5. The differing patterns of resistance to water vapour loss associated with egg shell cuticle are related to the ultrastructure of the cuticle itself, especially the number and size of cuticular fissures.

6. The results suggest that egg shell cuticle is a component in a series of resistances associated with the egg shell and that the use of Fick's Law is insufficient to explain water vapour diffusion across the shell.

7. Embryo survival may be improved by cuticle removal, though this may be limited to those species in which the cuticle restricts water vapour loss in initially low conductance egg shells.     

Whitening of brown shelled eggs: Individual variation and relationships with age,fearfulness, oviposition interval and stress

1. Fearfulness, shell colour, incidence and degree of shell whitening and the interval between ovipositions were studied in two populations of 30 brown egg laying hens with family histories of a low or a high incidence of egg shell whitening.

2. Hens of the population with the high incidence of whitening appeared to be more fearful than hens of the population with the low incidence of whitening.

3. Brown colouration of the egg shell and the incidence and degree of shell whitening declined as the hens aged.

4. Brown colouration and egg shell whitening were most pronounced on the blunt ends of the eggs.

5. A large part of the variation in egg shell whitening was attributable to the individual (hen) component of variance.

6. Differences in egg shell whitening, between the two populations, were detectable throughout the 26 weeks of the experiment.

7. Oviposition intervals were similar for normal and coated eggs when birds were not exposed to disturbance.

8. Disturbance of hens increased oviposition intervals and the incidence and degree of shell whitening, to a similar extent, in both populations.

9. It is concluded that stress‐related egg retention is not the sole factor responsible for abnormal egg shell whitening. Shell whitening may occur as a consequence of the premature termination of shell pigmentation as well as a consequence of the retardation of oviposition which occurs when hens are disturbed.     

Genotypic differences in speed and magnitude of response to ahemeral lighting

1. The paper compares between three genetically different lines in their egg weight and shell thickness response to a 28‐h cycle.

2. The response in shell thickness to ahemeral lighting was more rapid (2.2 d) than for egg weight (4.2 d).

3. Differences between genotypes were evident in both the speed and magnitude of response.

4. Overall, egg weight and shell thickness on the 28‐h cycle were 7% and 8% greater than on 24 h.

5. The allometric increase indicates that the increase in shell weight was on average 14% more than the increase in egg weight.     

The hen's egg: Incidence and inheritance of some egg abnormalities

1. More than 9000 eggs, each laid by a known hen, were examined and malformations of six types recorded: double‐yolked, A, B, rough, shell‐less and thin‐shelled.

2. A and B malformations are those seen in, respectively, an egg that is held in the shell gland for an abnormally long period and one that reaches the shell gland while an A‐egg is still there; many of the eggs recorded as rough‐shelled were probably A‐eggs for which the corresponding B‐eggs had been lost.

3. The birds were of three strains: a medium‐body‐weight brown‐egg‐laying strain and a White Leghorn strain, both of commercial origin, and a laboratory strain of Brown Leghorns.

4. The incidences of egg malformation in the three strains were 8.3, 9.2 and 0.5%.

5. A‐, B‐ and rough‐shelled eggs constituted more than two‐thirds of all malformed eggs.

6. Evidence from two generations suggests that these malformations have a high heritability.     

Studies on the avian shell gland during egg formation: Aqueous and electrolytic composition of the mucosa 1

1. The intracellular composition of the avian shell gland mucosa was studied at six stages of egg formation.

2. Total water content of the mucosa before shell calcification was 4.8 kg/kg dry weight (dw) and rose to 6.6 kg/kg dw during shell formation; 60% of the increase was intracellular and 40% extracellular.

3. The potassium concentration of the mucosa remained constant during egg formation.

4. Chloride was removed constantly from the mucosal cells during egg formation.

5. A model was constructed for the glandular cells which depicts them as primarily NaHCO₃‐secreting cells towards the luminal side and HC1‐secreting cells towards the serosal side, their main function being to provide CO^2‐ ₃ for shell formation. In this model, the columnar mucosal cells are responsible for the calcium used in shell formation.     

Responses of the reproductive vascular system during the egg‐formation cycle of unanaesthetised laying hens

1. Vascular adjustments to the process of egg formation were examined in the unanaesthetised laying hen, by the radioactive microspheres method.

2. Three‐ to four‐fold increases in blood flow were found in segments surrounding the egg during its passage along the oviduct, possibly due to an enhanced metabolic activity in the muscle layer of the oviduct.

3. Shell‐gland blood flow was minimal in the absence of an egg and increased gradually to a maximum (5‐fold) about 5 h after entrance of the egg into the shell gland. This parallels the rate of calcification of the egg shell.

4. Changes in blood flow in the ovarian follicles and other parts of the oviduct were small while the egg was in the shell gland. This might be typical for the reproductive system of the fowl, which undergoes little structural alteration during egg formation.     

Differences in egg‐shell quality among the Sinai Bedouin fowl,the commercial white leghorn and their crossbreds

1. A comparison of egg‐shell characteristics was made among the Sinai breed (a desert inhabiting strain), the commercial White Leghorn and their reciprocal crossbreds.

2. The Sinai egg was smaller and its shell thicker and stronger than the Leghorn egg. All the differences were statistically significant.

3. Shell thickness and strength of Leghorn eggs were in accordance with values predicted on the basis of egg mass, while those of the Sinai and the two crosses were considerably higher than predicted.

4. The apparent differences in mean egg mass between Sinai and Leghorn breeds did not explain the differences in egg‐shell quality, neither did the shape index which was very similar among all breeds.

5. The findings suggest that the differences in egg‐shell properties are of a genetic origin.

6. The thick, strong and less permeable egg‐shell of the Sinai breed may reflect adaptations to its arid environmental origin and to incubation in the open.

7. These genetically‐determined egg‐shell characteristics might serve in a future selection for improved egg‐shell quality in poultry.     

Physical characteristics of intact and cracked eggs

1. Six egg samples, comprising equal numbers of intact and cracked eggs, were collected from three flocks of hybrid layers.

2. Cracks were classified according to type (hole, star or straight) and the weight, shape, shell colour and specific gravity of all the eggs were measured. Shell thickness and dry shell weight were also recorded for a maximum of 10 intact and 10 cracked eggs from each sample.

3. The largest proportion of cracks were holes.

4. Differences in mean egg weight and shape of intact and cracked eggs within each sample were inconsistent. Mean shell colour was darker for the intact brown eggs than for the cracked ones. For all the samples, mean egg specific gravity, shell thickness and dry shell weight were higher for the intact eggs. The difference in egg specific gravity was significant (P<0.05) for all samples.

5. Holes occurred in eggs of lower mean weight but with better quality shells than those in which star or straight cracks occurred.     

Traits influencing the hatching performance of Japanese quail eggs

1. Japanese quail eggs from moderately heavier sires showed superior fertility; while fertile eggs from moderately heavier dams hatched slightly better than the eggs from lighter dams.

2. Higher rates of fertility and hatchability of Japanese quail eggs were observed from parents of 10 to 19 weeks of age, with peak fertility and hatchability at 14 and 12 weeks of age, respectively.

3. Sex ratios of 1:2 to 1:5 gave comparable fertility and hatchability results.

4. The hatching performance of quail eggs from cage and deep litter reared breeders was comparable.

5. Fertility and hatchability were directly proportional to the egg weight.

6. Quail egg shell colour, tints and blotches were found to influence hatching performance.

7. Storing quail eggs at 16 ± 2°C and 75 ± 5% relative humidity for more than 4 d reduced hatchability.

8. Hatchability of eggs stored at room temperature was improved if they were sealed in polyethylene bags.

9. Provision of light during the first 14 d of incubation resulted in a photo‐acceleration of about 3.2 h.     

Egg‐shell water Vapour conductance of the domestic fowl: Comparison between two breeds and their crosses

1. A comparison of egg water loss and egg‐shell water vapour conductance was made between the Sinai fowl (a desert inhabiting breed) and the commercial White Leghorn, together with their reciprocal crossbreds.

2. The Sinai egg was found to be smaller and less permeable to water vapour than the eggs of the Leghorn and crossbreds. The differences were statistically significant.

3. The measured egg‐shell water vapour conductance of the Sinai breed was 25% lower than predicted on the basis of egg mass.

4. The low permeability of the Sinai egg shell might be related to its higher than predicted thickness, which did not interfere with the shell functional pore area.

5. The low water vapour conductance of the Sinai egg shell may reflect adaptations to its dry habitat.     

Comparisons of frequencies and egg shell characteristics of broken and intact eggs within diverse populations of chickens

1. Differences were found among eight populations of White Plymouth Rock pullets in the frequency of broken eggs.

2. The shells of such eggs were thinner than those of intact eggs in seven of the populations.

3. The number of defective eggs, the specific gravity of the eggs, and the percentage hen‐day egg production of normal eggs were significantly correlated within populations with the incidence of broken eggs.

4. No significant correlations were found within populations between the incidence of egg breakage and either egg weight, body weight, or shank length.     

Plasma inorganic phosphorus concentration during egg‐shell formation: Effect of the physical form of the dietary calcium

1. Four experiments were carried out with Warren laying hens to elucidate the changes in plasma inorganic phosphorus (P_i) concentration during egg formation.

2. In hens receiving a normal diet containing a calcium supplement in a powdery form P_i increased from 25 to 42 mg/1 during an entire shell formation cycle (from 10 to 22 h after oviposition of the previous egg), while in cockerels P_i decreased slightly during the night.

3. This increase in P_i in hens, was not related to cessation of feeding at the onset of darkness but was specifically connected with the beginning of shell secretion.

4. When hens received calcium as crushed sea‐shells separately from the diet, the nocturnal peak in Pi virtually disappeared and only a temporary increase of 4 mg/1 between 10 and 14 h after oviposition remained.

5. These results indicate that the beginning of shell secretion is always accompanied by an increase in Pi and that a separate presentation of dietary calcium reduces the bone mobilisation at night.     

Influence of stocking density and layer age on production traits and egg quality in Japanese quail

1. Japanese quail hens were housed from 6 to 26 weeks of age in cages providing areas of 150, 180, 210 and 240 cm²/bird.

2. Body weight gain, age at 50% egg production, mortality, hen‐day egg production and food conversion values showed significant improvement with proportionate increase in cage space per layer.

3. Egg weight gradually increased with age but shell thickness was influenced neither by age of the hen nor by stocking density.

4. Yolk index and colour were superior in the lowest stocking density (more cage space) group; other egg quality traits and egg weight were not influenced by stocking density.

5. The albumen index, internal quality unit, yolk index and yolk colour values increased with age.     

Quality characteristics of quail eggs

1. An experiment was carried out with laying Japanese quail to study the influence of age on their egg quality.

2. Egg, yolk, albumen and shell plus membrane weights increased, whereas the egg shape, specific gravity and shell thickness decreased between 49 and 154 days of age.

3. Yolk, albumen and shell plus membrane comprised 32–59%, 53–57% and 7–80%, respectively of the total egg weight.

4. Regression between the quail's age and the characteristics of eggs were derived and correlation coefficients ranged from —0–42 to — 0–86. The albumen weight was more highly correlated with egg weight than yolk weight.

5. Relationships were also derived between egg shape and specific gravity, and egg shape and albumen weight.

6. It was concluded that the eggs’ characteristics were modified as the quails aged, and the best criterion of the shell's strength was the egg shape.     

Effect of spray sanitising on hatching egg cuticle efficacy and hatchability

1. Eggs from a broiler breeder flock were sanitised using a continuous spray sanitising machine. The effect of this treatment on the ability of the cuticle to resist water and bacterial penetration was investigated throughout the production cycle.

2. The volume of water taken up by eggs that had been sanitised was significantly (P < 0.001) greater than that taken up by the control eggs. The volume of water taken up by the sanitised eggs increased as the flock age increased, whereas water uptake was maximal for the control eggs at 42 weeks of age.

3. There was no correlation between water uptake values and shell thickness or egg weight for either the sanitised or control eggs.

4. Sanitised eggs tended to dominate the “poor cuticle quality” category at all flock ages, when a subjective assessment of cuticle quality was made using the stain Edicol Supra Pea Green.

5. Egg sanitising did not affect shell reflectance values, shell thickness or the percentage of cracked shells.

6. When flock age reached 49 weeks of age, the sanitising process significantly (P < 0.001) reduced hatchability.     

Structural flaws in some very strong egg shells

Egg shells from a particular bird gave very high values for strength at the broad pole when tested by an impact method.

Suitably prepared radial and tangential sections of these egg shells have been examined by polarised light and by histological staining techniques.

It was shown that these shells contained flaws which consisted of abnormal crystals embedded in the normal crystalline structure. The flaws contained abnormal organic material.     

Effect of probiotic supplementation on serum/yolk cholesterol and on egg shell thickness in layers

1. The effect of probiotic supplementation on egg production, on serum and yolk cholesterol and on egg shell thickness in 24 White Leghorn layers was studied from 28–38 weeks of age.

2. In 3 treatments the diet was supplemented with 0, 100 and 150 mg probiotic/kg food.

3. In the 100 mg probiotic group, egg production improved by 5%, and shell thickness improved slightly, with fewer thin‐shelled eggs than in the control (8.6% compared to 18.6%).

4. The initial serum cholesterol concentration of 170.2 mg/dl in control birds remained similar throughout the 10‐week experimental period, whereas in the 150 mg group the initial value of 176.5 mg/dl decreased to 114.3 mg by week 10.

5. Yolk cholesterol concentration was 14.69 mg in the control group and 11.28 and 11.37 mg/g in the 100 and 150 mg probiotic groups respectively. Overall mean total egg cholesterol was thus reduced by probiotic supplementation.     

Relationships between age,shell quality and individual rate and duration of shell formation in domestic hens

1. The duration and rate of shell formation was measured individually in each of 44 hens. Comparisons were made between hens of different ages, and also between hens which laid eggs of differing shell qualities.

2. Shell formation began 9–5 to 11 h after the oviposition of the previous egg, increased linearly for 13 h and then plateaued at 1–5 h before oviposition.

3. The variation in egg shell quality observed in two experimental groups of hens was 75% attributable to differences in the rate of shell deposition and 25% attributable to difference in the duration of shell deposition.

4. The mean interval between ovipositions increased with age but neither shell weight, nor rate or duration of shell depositon were affected. The increase in egg weight with age accounted for the decrease in shell quality.

5. Intervals between ovipositions were positively correlated with the duration of shell formation, especially its latter stages.

6. Egg production was negatively correlated with the interval between ovipositions but not with the duration or rate of shell deposition. There was a negative correlation between duration and rate of shell deposition, except in the case of the hens producing eggs of poor shell quality.     

The use of selection and strain crossing to reduce egg shell breakage

1. Selection for low egg shell deformation and high egg production was carried out in two White Leghorn strains, M and H, in order to test the feasibility of reducing the incidence of egg breakage without loss in egg production.

2. Selected and control lines were maintained within each strain.

3. After three generations of selection, egg shell deformation was reduced in the M selected line as compared to its control counterpart but this change in deformation was not accompanied by a reduction in egg breakage; however, no reduction in deformation or egg breakage was achieved in the H selected line.

4. Crossing the M and H strains resulted in a reduction of egg breakage in the crosses as compared to the average breakage of the parental strains, suggesting the potential of utilising strain crosses to reduce the incidence of egg shell breakage.     

Effect of gradual acclimation to high ambient temperatures on egg‐shell quality of the Sinai Bedouin fowl,the commercial white leghorn and their crossbreds

1. The effect of gradual acclimation to high ambient temperatures on egg‐shell quality was studied in the Sinai fowl, the commercial White Leghorn, and their reciprocal crossbreds.

2. The Leghorn egg was characterised by a thinner and weaker shell compared with the Sinai and the crossbreds, at all the experimental temperatures.

3. In contrast to other reports, high ambient temperatures for a long period had only mild effects on egg‐shell quality.

4. The results suggest that gradual acclimation to high ambient temperatures might improve the efficiency of the physiological mechanisms involved in the hen's response to heat. Consequently, the reproductive process adapts to the hot environmental conditions.

5. The results indicate that the Sinai breed might be used for future selection of a breed, highly resistant to extreme environmental conditions and with an improved shell quality.