The sparing action of inorganic sulphate on sulphur amino acids in practical broiler diets: Preliminary trials with young chicks 1

1. In three trials marked responses to Na₂SO₄ were obtained, both in terms of growth rate and of food/gain ratio. In a fourth trial the chicks responded to graded additions of synthetic methionine but not to inorganic sulphate.

2. The response of the chicks to Na₂SO₄ depended on the amount of sulphur amino acids in the diets, being absent or difficult to demonstrate in severely deficient diets or in those adequately supplemented, respectively.

3. Sodium sulphate cannot replace more than about 005% supplementary methionine.

4. Usually the efficacy of Na₂SO₄ in improving performance appeared to range from 40% to 60% that of synthetic methionine.     

Lupin and rapeseed meals in poultry diets: Effect on broiler performance and sensory evaluation of carcasses

1. The effect of including lupin meal (Lupinus angustifolius), with or without rapeseed meal, in computer‐formulated, least‐cost diets for broilers has, been determined.

2. In the first experiment the diets contained 0, 8 (with and without supplementary methionine, lysine, or methionine and lysine) or 16% lupin meal: addition of the lupin meal required a concomitant addition of 5 % rapeseed meal (by computer selection).

3. The diets were fed from 38 to 65 d and did not affect live‐weight but the efficiency of food utilisation was significantly worse on the 16 % lupin, 5% rapeseed meal diet than it was on the control diet.

4. The second experiment was designed as a 2 x 4 factorial to separate any effect due to rapeseed meal from that due to lupin meal, the treatments, which were applied from 1 to 62 d, being 0, 8, 16 and 24% lupin meal with and without 5 % rapeseed meal.

5. Birds gained more rapidly in the second experiment on diets containing 16 or 24% lupin meal whereas live‐weight was depressed when 5% rapeseed meal was included in the diets containing 0 or 8% lupin.

6. The 16 and 24% lupin treatments also had added dietary fat from 6 to 9 weeks of age and this may have ameliorated the adverse effect of rapeseed meal.

7. Up to 16% lupin meal and 5% rapeseed meal had no detectable effect on the colour, appearance, flavour or texture of roasted chicken meat when evaluated by a trained taste panel.     

Trace mineral interactions in broiler chicken diets

1.?The aim of the present study was to demonstrate trace mineral interactions among organic copper, iron, manganese and zinc (Cu, Fe, Mn and Zn) in broiler chickens.

2.?Three experiments were conducted using a control diet which was deficient in Cu, Fe, Mn and Zn.

3.?In experiment 1, the control diet, supplemental organic Cu, Fe alone and combined diets, were randomly fed to 4 groups of one-day-old Cobb broilers (each group had 6 replicates of 4 birds).

4.?In experiment 2, the control diet, supplemental organic Mn and Zn alone or combined with Cu, Fe diets and corresponding inorganic combined diet, were randomly fed to 6 groups (each group had 8 replicates of 6 birds).

5.?In experiment 3, the depletion of organic Zn, the depletion of inorganic Zn and normal Zn treatments were carried out in three groups of one-day-old Cobb broilers (each group had 8 replicates of 6 birds).

6.?Adding organic Cu, Fe and Mn alone or combined to Zn deficient diets did not significantly improve bird performance and were mostly excreted. Supplemental organic Zn alone or combined with other elements significantly increased feed intake, body weight gain and tibia bone length. However, supplemental organic Fe alone or combined with Cu significantly increased feed intake but had no obvious effect on body weight gain. The organic Fe supplementation resulted in a wider tibia.

7.?Depletion of organic and inorganic Zn resulted in decreased feed intake, body weight gain and total tibia bone Zn content. Zinc deficiency did not affect the uptake of organic Fe by tibia bone but reduced its total Fe content.

8.?Zinc is the first limiting element among these 4 trace minerals. Adding Mn, Cu and Fe to Zn deficient diets did not stimulate bird performance. Surplus organic Fe and Cu resulted in increased feed intake and increased tibia bone Fe content but did not contribute to bird performance.     

Replacement of maize by triticale in broiler diets

1. The nitrogen‐corrected metabolisable energy contents of the triticale varieties Multi Graze, Graze Grain 70, 204 and 131 were 12.28, 12.69, 12.37 and 12.03 MJ/kg respectively.

2. Increasing proportions (0, 25, 50 or 75%) of the maize of a diet were replaced by triticale 131 to provide diets of equal energy and nitrogen contents for broilers. These diets resulted in weight gains of 1.775, 1.782, 1.713 and 1.808 kg respectively at 8 weeks and there were no significant differences among efficiencies of food conversions.

3. Dressed carcass weights as percentages of body weight were 74.38, 75.01, 73.98 and 74.50 respectively and did not differ significantly.

4. Triticale in the diet did not seem to influence the relative weights of either liver or gizzard.

5. The flavour of the meat seemed to become less acceptable as the triticale in the diet increased but there were no significant differences in tenderness and juiciness of the meat caused by the treatments.     

Methionine supplementation of low protein diets for broiler chicks in the tropics

1. In the first experiment, diets containing 14, 16, 18, 20, 22 or 24% protein with no methionine supplementation were tested, while in the second experiment diets containing 16, 18 and 20% supplemented with methionine to give 90, 100 or 110% of the recommended N.R.C. levels were compared with an unsupplemented 24% protein diet.

2. Methionine supplementation of the low protein diets stimulated faster gains than the unsupplemented diets but not enough to surpass the response to the 24% protein control diet.

3. It was concluded that a high protein diet unsupplemented with methionine was more economical to feed to broilers than supplemented low protein diets.     

Xylanase in broiler diets with differences in characteristics and content of wheat

1. Experiments were conducted to measure the effect of xy‐lanase supplementation on the performance of broilers fed on wheat‐based diets containing high and low extract viscosity wheat (experiment 1), baking and feeding quality wheat (experiment 2) and with variation in wheat content (experiment 3).

2. Xylanase supplementation improved food conversion ratios by 2·2–2·9% and body weight gain by 0·2–2·5%. This was independent of wheat characteristics but slightly dependent on the wheat content of the diet. These effects were related to a lowering of digesta viscosity.

3. Differences in extract viscosity of wheat were not reflected in broiler digesta viscosity.

4. Reduction of digesta viscosity resulted in only a slight improvement of litter score. Reduced intestinal viscosity decreased relative pancreas weight and to a lesser extent relative small intestinal weight.

5. Baking quality wheat Baldus gave better performance than the feed quality wheats Apollo and Slejpner.

6. It was concluded that xylanase supplementation of wheat‐based diets invariably had a positive effect on broiler performance.     

Guar meal in diets for broiler chickens

1. In two experiments each involving 2 000 Ross 1 broiler chickens in floor pens from 0 to 56 d of age, the effects of including guar meal at 50, 100 or 150 g/kg of the diet were investigated.

2. During the 0 to 28‐d period diets containing 50 or 100 g guar meal/kg supported only 85 and 69%, respectively, of the growth supported by the control diet, whereas during 28 to 56 d, birds fed on diets containing 100 or 150 g guar meal/kg gained 90 and 86% of the weight gained by control birds.

3. Neither toasting the meal, steam pelleting diets containing the meal nor supplementing these diets with 5 g methionine/kg had any appreciable effect on performance.

4. Addition of either of two enzyme preparations, MKC hemicellulase or betaganase M, improved growth; birds receiving 100 or 150 g guar meal/kg gaining 96 and 89%, respectively, of the weight gained by control birds from 28 to 56 d of age.     

Economic effects of increased vitamin E supplementation of broiler diets on commercial broiler production

1. The economic effects of increased vitamin E supplementation in 168 commercial broiler flocks incorporating over 3 million birds was assessed using a computerised data retrieval system.

2. Approximately half of the flocks were fed on either a high (163 mg/kg) vitamin E or on a normal (44 mg/kg) vitamin E‐containing diet throughout production.

3. Approximately half of the flocks were the progeny of breeder flocks which had been fed on a high vitamin E‐containing diet throughout production.

4. The improvement in target income in the vitamin E‐supplemented broiler flocks was 8–44% (P < 0.01).

5. The residual improvement in target income in these flocks, after the cost of the additional vitamin E was taken into account, was 2.74% and failed to reach statistical significance.

6. The increased net income was achieved by a significantly improved food conversion ratio and average weight per bird (P < 0.05).

7. There was no overall effect of vitamin E supplementation of broiler breeders on subsequent broiler performance.     

The effect of sodium sulphate and methionine on the utilisation of non‐protein nitrogen by laying hens

1. The response of layers to adding diammonium citrate (DAC), sodium sulphate or methionine to a basal diet containing 136–3 g protein/kg was determined.

2. Supplementing the diet with DAC equivalent to 25 g protein/kg did not improve egg production, the efficiency of food utilisation, egg weight, nitrogen retention or the apparent absorption of lysine and methionine; increases in food intake and in the concentration of methionine in the serum and liver were observed.

3. Adding sodium sulphate, alone or with DAC, did not affect the variables noted above.

4. Supplementation of the basal diet with methionine increased egg production, egg weight, food intake and the concentrations of lysine in the serum and liver.

5. It is concluded that the supplemental NPN was used only in serum protein synthesis.     

Studies on the incorporation of triticale in diets for growing chicks

1. The classical ME value of triticale varied from 12.305 to 13.778 kJ/g or 11.819 to 13.016 kJ/g corrected for N‐retention depending upon method of calculation.

2. Substituting triticale for maize, weight for weight, but not on a iso‐nitrogenous basis, in a groundnut oil cake (GNC) starter diet improved weight gain (P < 0.05) when the substitution exceeded 75% without affecting the protein efficiency ratio (PER).

3. A similar substitution in a soybean oil meal diet, improved weight gain (P < 0.05) at 50% or more but PER declined (P < 0.05).

4. The net protein utilisation and protein retention efficiency values of the diets in which maize protein was replaced by triticale protein were significantly reduced.

5. The maize‐GNC diet was equally limiting in methionine and lysine while the triticale‐GNC diet was not.

6. The growth and food efficiency obtained with a triticale‐GNC diet was higher (P < 0.01) than with the maize‐GNC diet but not with a maize‐GNC supplemented with fish meal and methionine.

7. It is concluded that triticale could quantitatively substitute maize in the starter diets.     

Performance of laying hens fed on low sulphur amino acids diets supplemented with choline and methionine

1. Two experiments were carried out to examine the possibility of replacing a part of the methionine content with choline in a layers’ diet.

2. In the first a low methionine diet with 2.6 g methionine/kg was supplemented with 0.3, 0.6, 0.9 and 1.2 g DL‐methionine/kg. Choline at 1.12 g/kg was added to the diets, which contained 5.4, 6.0 and 6.6 g TSAA.

3. In the second diets with 3.02, 3.80, 4.53, 5.30, 6.04 and 6.51 g TSAA/kg were used and 0.94 g choline/kg was added to these diets.

4. Supplemental methionine increased egg production but not egg weight in the first experiment. When the low TSAA diet was supplemented with choline, egg output increased slightly but not significantly.

5. When supplemental methionine was added to the low protein and TSAA diets in experiment 2 it increased egg production and egg weight. Added choline did not increase egg production or egg weight, even with the diet very low in protein and TSAA. Body weight gain, food intake and feed conversion efficiency of food utilisation were improved by supplemental methionine but not choline.     

The nutritional value of algae for poultry. Dried chlorella in layer diets

1. The nutritional value of a sample of algae meal, mainly Chlorella, was assessed in an experiment with laying hens. The algae meal was that described by Lipstein and Hurwitz (1980).

2. Diets formulated by linear programming contained up to 120 g algae meal/kg with supplementary DL‐methionine.

3. There were no significant effects of dietary algae meal on egg output, food conversion or egg‐shell quality. Thus algae meal at the concentrations tested can be a useful substitute for soyabean meal in diets for laying hens.

4. High concentrations of dietary algae meal caused a deep yellow yolk colour of acceptable appearance.     

The effect of heat treatment and enzyme supplementation on the nutritive value of barley for broiler chicks

1. The effect of heat treatment and enzyme supplementation on the nutritive value of barley was studied.

2. In only one experiment was weight gain significantly improved when the barley was heated.

3. Autoclaving barley at 120 °C for 30 min reduced dietary dry matter digestibility and treating the barley with dilute acid before heating had no effect on its nutritive value.

4. Supplementing diets containing commercial barley with a‐amylase produced slightly conflicting results in that there was an improved weight gain, food conversion efficiency and digestibility value in two of three experiments.

5. The digestibility and metabolisable energy values of a North American six‐row spring barley (Glacier) were significantly improved by enzyme supplementation.

6. The effect of the enzyme on diets containing a high amylose barley (Glacier Pentlandfield) was positive but not significant.     

Rapeseed meals in broiler diets: Effect on performance and sensory evaluation of carcasses

1. The effect of including rapeseed meal (RSM) in lieu of soyabean meal in broiler diets was determined.

2. Live‐weight gains, from 8 to 63 d, were depressed when the diet contained 150 g RSM/kg and there was a highly significant negative correlation between weight gain and inclusion rate of RSM over this period.

3. The efficiency of food utilisation was worse when the diet included 150 g solvent RSM/kg than when it contained no RSM: food efficiency was consistently poorer whenever RSM was included in the diet but the difference was significant only when the diet contained 10 g tallow/kg.

4. Carcasses from birds fed on diets containing 10 g tallow/kg had a better appearance than those from birds on diets containing 30 g tallow/kg, while the flavour of meat from birds fed on a diet containing 150 g solvent RSM/kg treatment was adversely affected. However, up to 100 g solvent RSM/kg may be included in broiler diets without affecting appearance, taste, texture or acceptability of the meat.

5. Inclusion of 50 g or more, solvent RSM/kg reduced growth rate and worsened food efficiency.     

The effects of crude and refined low erucic acid rapeseed oils in diets for broiler chickens

1. The effects of feeding diets containing 15% of low erucic acid Zephyr rapeseed oil (B. napus) and of crude, refined and hydrogenated Span rapeseed oils (B. campestris) on growth and on tissue lipids of female broilers were determined.

2. Feeding refined or hydrogenated Span oil led to smaller body weights than feeding soybean oil, while refined Zephyr oil and crude Span oil supported a growth response similar to soybean oil.

3. The treatments had significant effects on the total lipid contents of heart (P ≤ 0.05) and spleen (P ≤ 0.001) but not liver.

4. There was no indication of lipid accumulation in the tissues of the chickens fed low erucic acid rapeseed oil as compared with those of the birds fed soybean oil.

5. Only small amounts of erucic acid were incorporated into heart, liver or spleen lipids when rapeseed oil was fed.     

Grass meal as an ingredient in diets for broiler chickens

1. Six diets of equal energy and nitrogen contents containing between 0 (control) and 125 g grass meal/kg were each fed to 4 groups of 42 male and 4 groups of 42 female broiler chicks, in floor pens, from 0 to 56 d of age.

2. Increments of 25 g grass meal/kg and 7.3 g maize oil/kg replaced a mixture of soyabean, wheat and barley meals in the control diet.

3. Gain: food ratio (y) of the birds decreased linearly as dietary grass meal content (x) increased such that y = 0.4295 ? 0.0032x.

4. Carcass analyses indicated that the decrease in gain : food ratio was caused by less deposition of body fat.

5. Skin colour became significantly yellower as the dietary grass meal content increased.     

Encapsulated benzoic acid supplementation in broiler diets influences gut bacterial composition and activity

1. The present study investigated the effects of encapsulated benzoic acid (BA) supplementation in broiler feed on performance and gastrointestinal microbiota.

2. Eighty broilers were randomly divided into two groups. Birds in the control group were fed on maize–soybean-based diets. Birds in the treatment group were provided the same diet supplemented with 2 g/kg BA encapsulated in a vegetable oil matrix.

3. At the end of the trial (d 35), pH, bacterial composition and metabolites were determined in the crop, jejunum, ileum and caecum.

4. Growth performance variables and pH were not significantly different.

5. BA concentration decreased rapidly in the proximal gut. However, the treatment diet showed higher BA in the crop, jejunum, ileum and caecum.

6. Total lactate in the crop and D-lactate in the jejunum was higher in the BA treated group. Caecal total and branched chain fatty acids were decreased due to the treatment.

7. Lactobacilli populations were significantly altered by BA supplementation. A trend for increased lactobacilli was observed in the crop, while it became significant in the jejunum and ileum. Lactobacillus species responded differently to the treatment. Four of 5 measured Lactobacillus species, particularly in the ileum, followed the course observed for total lactobacilli; only Lactobacillus salivarius was not modified.

8. Correlation analysis showed that BA modified the intestinal microbiota. Lactobacilli correlated negatively to all studied clostridial clusters and enterobacteria. Clostridial clusters IV and XIVa were significantly increased in the jejunum, whereas only clostridial cluster XIVa was increased in the caecum.

9. Encapsulated BA modified the intestinal microbiota which can lead to the conclusion, that the main beneficial mode of action of BA in the gut appears to be the enhancement of lactic acid bacteria, which in turn may act as a vanguard against pathogens.     

The necessity of supplementing laying hen diets with linoleic acid

Results from four experiments with laying hens have been re‐examined using the means of individual 28‐d periods during egg production to investigate the influence of the developing ovary and linoleate storage during growth on the linoleic acid requirement of the laying hen.

Apart from a possible beneficial effect during the first few weeks of production, little increase in egg weight occurs when birds are fed on conventional rearing diets and then fed on laying diets containing linoleic acid in excess of requirement.

After depletion of linoleate reserves, dietary supplementation with linoleic acid significantly increases egg weight at any age.

The necessity of supplementing diets for laying hens with linoleic acid is discussed.     

Effect of increasing cellulose in diets fed as crumbles or mash on the food intake and weight gains of broiler and Leghorn chicks

1. Two‐week‐old broiler and Leghorn cockerels were fed on diets containing 0, 100, 200, 300, 400, 500 or 600 g cellulose/kg in either crumble or mash form for 4 weeks.

2. Intake of the mash form of the cellulose‐diluted diets and associated body weights were less than those associated with the equivalent crumble diets which may have been due to decreased palatability of the former.

3. Broilers were unable to compensate for dietary dilution with cellulose by increasing food intake sufficiently to maintain nutrient intake with either mash or crumbled diets at any dilution.

4. At 100 g cellulose/kg for the mash diets and 100 and 200 g/kg for crumbled diets, Leghorns increased volume ingested by as much as 40% and maintained body‐weight gain and nutrient intake.

5. The results could be of value in designing feeding programmes for egg‐and meat‐type pullets.     

Phosphorus requirements of poultry. V. The effects during the subsequent laying year of feeding growing diets containing no animal protein or supplementary phosphorus

Pullets reared to 18 weeks of age on diets containing no feeding stuffs of animal origin and without phosphorus supplementation showed no significant difference in egg yield compared with similar pullets fed rearing diets containing animal protein and added phosphorus.

During the laying stage, diets in commercial use at this centre gave significantly higher egg yields, better average egg prices due to superior grading results and a higher margin over food cost than the same diets modified to conform with the nutrient recommendations of the National Research Council of America (1960).

Where the supplementary protein in both types of layers' diet was of vegetable origin, egg yield, food cost and margin of income over food cost were all significantly inferior to groups fed comparable diets containing supplementary protein of both vegetable and animal origin.

Soyabean meal and sunflower seed meal gave significantly poorer results when used in combination as the sole protein supplement compared with soyabean meal plus methionine.