Voluntary food restriction by laying hens mediated through dietary self‐selection

1. Individually caged Single Comb White Leghorn hens simultaneously received two diets which allowed selection of certain nutrients: these “ split‐diets “, essentially provided concentrated sources of either protein and energy (191 g crude protein, 12.82 MJ ME and 4.7 g Ca/kg diet), or calcium (107 g CP, 7.28 MJ ME and 131 g Ca/kg).

2. During four, 28‐d periods of lay, birds offered these split‐diets consumed some 7% less food in total than did control birds receiving a conventional diet ad libitum.

3. Calculation of nutrient intakes showed that birds on the split‐diets consumed significantly less protein, energy and calcium than the control birds.

4. Giving split‐diets also resulted in superior shell quality; treatment differences were also noted in the timing of oviposition.

5. It is suggested that the voluntary reduction in food intake noted for birds offered split‐diets is associated with an appetite for calcium.     

Improvement of dietary protein utilisation in chicks by medium chain triglyceride

1. To improve the influence of variation of ME intake, an experiment was carried out using equalised feeding to investigate the comparative effects on protein and energy utilisation in chicks of diets containing medium chain triglyceride (MCT) and long chain triglyceride (LCT). Experimental diets were given at 3 different food intakes, namely, 100, 120 or 147 g/bird/10 d. The diets contained MCT or LCT on an isoenergetic basis. Maize oil and caprylic acid triglyceride respectively, were used as LCT and MCT sources.

2. Body weight gain and food efficiency of chicks significantly increased with the supplement of dietary MCT compared with dietary LCT at all food intakes. Protein retention and the efficiency of protein utilisation (protein retained/protein intake) at all food intakes also significantly increased with dietary MCT, while body fat and fat retention were significantly reduced. Chicks fed the LCT‐supple‐mented diet, on the other hand, had a lower protein retention, but significantly higher fat retention. The value for energy retention and the efficiency of energy utilisation (energy retained/ME intake) were not significantly different between MCT‐ and LCT‐supplemented diets.

3. It was concluded that supplementing MCT to the chick diet would improve body weight gain and protein utilisation while regulating fat deposition compared to the LCT supplemented diet, under equalised feeding conditions.     

Influence of activity and dietary energy on broiler performance,carcase yield and sensory quality

1. A total of 2560 male and female Ross broilers were raised to 42 days of age in a 2 × 2 treatment factorial arrangement experiment to investigate the influence of different degrees of physical activity and dietary energy on broiler performance, abdominal fat content, carcase yield and sensory quality.

2. Vertical fans were used to force the treatment birds to walk 3 to 4 times as far as the normal activity birds; birds were fed a normal and a high energy diet (12.55 compared with 13.81 MJ ME/kg) with the same energy/protein, energy/lysine and energy/methionine + cystine ratios.

3. High activity birds had greater body weight ( + 4.1%), food intake ( + 5.1%) and ME intake ( + 5.1%) than normal activity birds. Birds receiving high energy diet had a lower food conversion and food intake than birds receiving normal energy diet. There were no significant differences in body weight or ME intake between birds with different diets.

4. Slaughter yields, both absolute and relative to live body weight, were affected by activity or dietary energy to varying degrees. Breast meat was increased with more activity. The absolute weight of abdominal fat was independent of activity and in males the relative weight of abdominal fat was decreased in high activity birds.

5. Different degrees of activity and dietary energy had only minor influences on broilers' sensory quality.     

The effects of dietary intake and of dietary concentration of copper sulphate on the laying domestic fowl: Effects on some aspects of lipid,carbohydrate and amino acid metabolism

1. These investigations were carried out to determine whether the effects of feeding CuSO₄‐supplemented diets on metabolism in the laying hen were due directly to the dietary CuSO₄ content or indirectly to the effects of CuSO₄ on food consumption.

2. In experiment 1, 144 hens were given GuSO₄‐supplemented diets ad libitum for 48 d. Food consumption decreased as the dietary CuSO₄ content increased and this was associated with decreases in blood plasma lipid, 17β‐oestradiol and liver lipid concentrations and hepatic lipogenic enzyme activities.

3. In experiment 2, 60 laying hens were subjected to three dietary regimens in an attempt to differentiate between the effects of reduced food consumption and dietary CuSO₄ per se. Control and CuSO₄‐supplemented diets were fed ad libitum, or the CuSO₄‐containing diets were force‐fed to the same intake as the ad libitum control diet, or the control diet was pair‐fed to the same intakes as the CuSO₄‐supplemented diets. Liver lipid concentrations were significantly decreased in the ad libitum and pair‐fed groups at the lowest intake. Plasma 17β‐oestradiol concentration was decreased in ad libitum and force‐fed birds given diets with 1 000 mg added Cu/kg. Lipogenic enzyme activities were reduced in the ad libitum and pair‐fed groups and increased in the force‐fed birds.

4. The results indicate that CuSO₄ per se directly affected circulating 17β‐oestradiol, and hence reproductive physiology, and that the quantity of food consumed influenced the rate of hepatic lipogenesis.     

Effects of partially hydrolysed guar gum on feeding behaviour and crop emptying rate in chicks

1. The effects of partially hydrolysed guar gum (PHGG) or intact guar gum (GG) on feeding behaviour and crop emptying rate in growing chicks were investigated.

2. Several combinations of dietary PHGG and GG at 50 g per kg diets were prepared for a feed intake experiment. Birds (17‐d‐old) were given diets for 3 h after 16 h fasting, and food consumption was measured at 1 h intervals. The food intake rapidly decreased as the dietary GG content increased even at 1 h after feeding.

3. The rate of food passage from the crop was also investigated with birds (20‐d‐old) after 16 h fasting. Birds were tube‐fed diets having several ratios of dietary PHGG and GG. After 1 h of feeding, the diet remaining in the crop was measured after drying. The crop emptying rate decreased linearly as dietary PHGG concentration decreased.

4. The present study suggests that partial hydrolysis of dietary GG improve both feeding behaviour and food passage from the crop in growing chicks.     

Effect of inclusion of sugar beet pulp,pelleting and season on laying hen performance

1. The effect of three metabolisable energy (ME) concentrations 12.2, 11.3 and 10.5 MJ ME/kg and two forms of diet (mash and pellet) on egg production in summer and winter were studied. The different ME values were obtained by diluting the 12.2 MJ ME/kg diet with sugar beet pulp of 2.88 MJ/kg DM. In a 3rd summer experiment the water intake of hens on these diets was examined. Data were analysed by factorial analysis of variance, and the relationship between performance and dietary energy concentration was examined by regression analysis.

2. The use of low energy diets (10.5 to 11.3 MJ ME/kg) decreased egg production in both seasons, but pelleting diets improved egg production and egg weight in summer. Egg weight was not affected by energy density in winter, but decreased in the summer when energy density increased.

3. In winter, food consumption was not affected by the dietary ME, while in summer food consumption increased when the dietary ME increased. Therefore, energy intake was not the same at all energy densities, but tended to increase as the energy density increased.

4. In summer, pelleting improved food efficiency and egg production and increased water consumption and the ratio of water to food intake. Water intake and water: food ratio decreased with increasing dietary energy concentration.     

Dietary selection of protein and energy by pullets and broilers

1. White Leghorn pullets and sexed broilers were allowed a free choice of two “ split‐diets “ which were concentrated sources of either crude protein (463 g/kg diet) or energy (13.32 to 14.00 MJ/kg diet).

2. Pullets receiving these two diets displayed a slower, but more uniform growth rate than did birds offered a single conventional diet. Up to 11 weeks of age, control birds consumed significantly more protein while the converse was true from 11 to 20 weeks. These differences are discussed in relation to the stage of sexual maturity.

3. Broilers offered the split‐diets grew more slowly and had an inferior food conversion ratio compared with control birds fed on a two‐stage rearing programme.

4. Among the broilers offered split‐diets, the usual sex differences were not observed for weight gain or carcass fat content.     

A study of the effect of variation in the calorie‐protein ratio of a medium energy diet and a high energy diet containing sucrose on the efficiency of egg production in caged layers

Literature pertaining to the variation in the protein content of layers’ diets is discussed and the lack of information on the effect of calorie‐protein ratio on food conversion is mentioned. The experiment described in the present paper was designed to test the effect of variations in calorie‐protein ratio on food conversion on a diet of high energy content (1340 k cal/lb.) based on sucrose supplementation and on a medium energy diet (1160 k cal/lb.). At the same time three types of pullet, Thornber 606, Thornber 404 and Light Sussex x Brown Leghorn pullets were compared on the two types of diet, each being fed at three nominal protein levels of 13, 15 and 17 per cent giving a total of eighteen treatments. The pullets were individually recorded for egg production, egg weight and food consumption. Meta‐bolisable energy crude protein determinations were carried out on each of the six diets employed and the actual calorie‐protein ratios calculated.

There was no significant effect on egg production when the calorie‐protein ratio of the diet was raised from 69 (i.e. k cal M.E./lb. per 1 per cent protein) to 90 but an increase to 103 reduced production and egg weight. It was found that maximum efficiency of food conversion was realised at ratios of 76 to 78.

The efficiency of conversion of dietary energy was unaffected by energy concentration in the diet.

The lightweight hybrid (Thornber 606) produced eggs more efficiently than either the Thornber 404 or the crossbred type.     

The effects of dietary intake and of dietary concentration of copper sulphate on the laying domestic fowl: Effects on laying performance and tissue mineral contents

1. Sixty white hybrid (Shaver 288) laying hens were subjected to three main dietary regimens for 5 d: ad libitum feeding of control and CuSO₄‐supplemented diets, force‐feeding these same diets to the same intake as that of the ad libitum control diet and pair‐feeding the control CuSO₄‐free diet to intakes equivalent to those of supplemented diets.

2. Final body weight of the force‐fed birds was significantly greater than of the other groups. Egg number and weight were significantly reduced as food intake decreased.

3. Liver weight per unit body weight was significantly increased and oviduct and ovary weights/kg body weight were significantly reduced by force‐feeding. As food intake decreased kidney and gizzard weights per unit body weight were significantly increased.

4. The concentrations and total contents of Cu and Zn in liver were significantly increased in the ad libitum and force‐fed groups.

5. Force‐feeding CuSO₄‐supplemented diets, which eliminated difference in food intake, confirmed that CuSO₄ per se affected tissue weights and mineral concentrations as distinct from effects due to the reduced food intake associated with increasing dietary CuSO₄.     

Effects of restricting the amounts of medium or low‐energy diets fed to laying hens in different colony sizes

1. Light hybrid hens between 32 and 68 weeks of age kept 4, 5 or 8 birds to the cage received one of three energy intakes, from a medium‐ or low‐energy diet.

2. Ad libitum fed birds laid more and heavier eggs than any of those restricted. A decrease in daily egg output of about 5 g was associated with each 100 kJ decrease in daily intake of metabolisable energy.

3. For the same energy intake, egg output of birds fed on the low‐energy diet was markedly greater than that associated with the medium‐energy diet.

4. The mortality of birds receiving restricted quantities of the medium‐energy diet was two to three times greater than that associated with similar rationing of the low‐energy diet.     

Synthetic oestrogens in the production of roasting fowl. II. Dietary nutrient concentration and time of implantation

Three experiments have been carried out using broiler‐type birds reared beyond the normal broiler stage to 14 weeks of age. In the first experiment control cockerels were compared with birds injected with either a 15 mg. hexoestrol pellet or a 15 mg. pellet of diethylstilboestrol at 9 weeks of age: at this stage three diets of different nutrient density were offered. As the nutrient density was increased, food utilisation progressively improved and growth rates were raised. Hexoestrol implantation resulted in an increase in voluntary food intake of 23 per cent above the control whereas diethylstilboestrol implantation increased voluntary food intake by only 14 per cent. These increases in food intake were associated with proportional increases in live‐weight gain.

In a second experiment pullets received 15 mg. implants of hexoestrol at 64 days of age, and were then fed diets of different nutrient concentrations to 98 days of age. Live‐weight gain tended to improve as nutrient density was raised, and efficiency of food utilisation was inversely proportional to nutrient density.

In a third experiment male birds were reared from 70 to 119 days of age. Four different implantations with hexoestrol at various ages were compared: only in those receiving two implants was there any difference in performance. Six diets varying in energy level and protein level were offered. Growth rates improved as dietary protein levels were raised progressively from 13.3 to 16.3 per cent, though food utilisation was most efficient at 14.8 and 16.3 per cent crude protein. Growth rate was 7.6 per cent greater when the dietary energy level was raised from 3065 to 3275 kcal. metabolisable energy per kg.     

A quantitative analysis of the literature concerning the restricted feeding of growing pullets

The sexual maturity of growing pullets can be retarded either by restricting calorie intake or by feeding a diet deficient in one or more essential amino acids, Several methods have been proposed for achieving either of these objectives. In this review the methods have been classified as (a) limiting the birds’ time of access to food, (b) quantitative food restriction, (c) the use of low energy diets and (d) dietary protein restriction.

Food restriction by limiting the time of access to food has given inconsistent results and is an unsatisfactory technique. Birds quickly learn to eat normal quantities of food in a very short space of time.

Quantitative food restriction has been widely used in experiments and is usually applied from about 6 to about 20 weeks of age. The available evidence enables relationships to be established between the degree of food restriction and subsequent body weight and sexual maturity. The effect of rearing treatment on mortality and rate of lay is also examined.

The ad libitum feeding of a low‐energy growing diet has always resulted in greater rearing food costs. This is because moderate reductions in energy content of the diet are offset by higher food intake. Diets sufficiently low in energy to cause a reduction in calorie intake will involve the purchase and handling of very large proportions of non‐nutritive material.

The use of protein‐deficient diets during the growing period, or from one day of age, offers a suitable means of delaying sexual maturity. The effects of such a treatment are apparently very similar to those of restricted feeding, but there is a lack of suitable comparisons of the alternative methods.     

Food intake,digestibility and gut size in red grouse

1. Fully‐grown captive red grouse were fed 4 increasingly fibrous diets which incorporated ling heather (Calluna vulgaris), their main natural food, at rates of 50, 200, 400 and 600 g/kg. The rest of each diet was a grouse maintenance ration.

2. Gizzard weights showed a close linear relationship with voluntary food intake. Previously published data for wild grouse fell close to the regression line.

3. Birds eating the 600 g heather/kg diet digested it more efficiently than expected by extrapolation from the three lower‐fibre diets. Their voluntary intakes of food varied more and they had longer caeca than birds on the lower‐fibre diets.

4. It was concluded that grouse began to show increased gut lengths and increased abilities to digest fibrous food when the intrinsic digestibility of their diet dropped below a certain, predictable, point. This may have been when bulk began to limit intake.     

Dietary essential oils improve the hepatic antioxidative status of broiler chickens

1. A total of 200 male Ross 308 chickens were used to evaluate the effects of a standardised combination of essential oils including 5% carvacrol, 3% cinnamaldehyde and 2% capsicum oleoresin (XT 6930; Pancosma S.A., Geneva, Switzerland) on their performance, hepatic antioxidant concentration and caecal tonsils morphometry.

2. Two diets were offered to broiler chickens from d old to 21 d of age. The control diet (C) was slightly lower in metabolisable energy (12.13 MJ/kg ME) and crude protein (215 g/kg CP) than breeders’ recommendation. The second diet, made as XT 6930, was added on the top of the control diet at 100 mg/kg. Each diet was offered ad libitum to birds housed in one of 10 floor pens in a randomised complete block design. The birds were housed in 20 floor pens, 10 birds in each pen, and were allocated to 10 replicates of the two dietary treatments.

3. The concentration of antioxidants in the liver of the birds was determined using high-performance liquid chromatography (HPLC) at 21 d of age. Birds fed control diet only had lower weight and converted less efficiently feed to gain compared to birds fed essential oils-supplemented diet. Feed consumption was not affected by dietary treatments. The antioxidant data showed that supplemented essential oils improved the hepatic concentration of carotenoids and coenzyme Q₁₀ when fed to broiler chickens. The morphometry of the caecal tonsils of the birds was not influenced by dietary treatments.

4. It can be concluded that that dietary combination of essential oils, including carvacrol, cinnamaldehyde and capsicum oleoresin, improved growth, feed efficiency and the hepatic concentration of carotenoids and coenzyme Q₁₀ when fed to broiler chickens.     

Calcium and phosphorus levels in the diet of laying birds

Two hatches of crossbred pullet chicks were brooded and reared to 18 weeks of age on diets which conformed to the recommendations of the National Research Council (N.R.C.) of America for calcium and phosphorus. The pullets of each hatch were then divided at random into six laying groups. Three groups were fed diets containing 2.25, 3.14 and 5.0 per cent respectively of calcium without supplementary phosphorus and the other three groups were fed diets of the same graded calcium levels but with supplementary phosphorus to give a total inorganic phosphorus content of 0.45 per cent. When the rate of egg production in the groups receiving 2.25 per cent calcium exceeded 70 per cent an ad libitum supply of limestone grit was provided and the intake recorded. Over the twelve months of lay this resulted in an average total dietary intake level of nearly 2.65 per cent calcium.

Egg yield was not significantly influenced by the diets, except that the production of birds fed on the diet containing 5 per cent calcium without supplementary phosphorus was significantly depressed. Food intake was not adversely affected by the higher levels of ground limestone; in fact, there was a positive regression of food consumption on the percentage of calcium in the diet. Because of the greater food intake and the lower egg production, food consumed per dozen eggs and cost of food per dozen eggs were higher in the groups receiving 5 per cent dietary calcium without phosphorus supplementation, compared with groups which received lower dietary levels of calcium. There were, however, no significant differences between the various groups in income or in the margin of income over costs, per bird or per dozen eggs.

The data on egg grading showed that over the two experiments and at all levels of calcium there was no evidence of any influence of phosphorus on the combined percentage of large and standard eggs. The average percentage for the two diets with the 5 per cent level of calcium was significantly greater than the combined average for all the other diets.

The average percentage of cracked eggs for the groups with diets containing 5 per cent calcium was 9.4 per cent compared with an average of 11.1 per cent for all the other diets, but the difference was not significant.

The results suggest that there is no economic advantage in raising the calcium content of the basal diet used in these experiments beyond the level of 3.14 per cent and that at this level there appears to be no need with this basal diet to supplement the phosphorus content.     

Intermittent lighting and feeding schedules for broilers incorporating long periods of darkness

1. Intermittent lighting and feeding schedules incorporating long periods of darkness and periods without access to food were applied to growing broilers.

2. Birds exposed to a 2 h light(L): 10 h dark(D) lighting schedule with feeding restricted to the light periods ate significantly less food and grew more slowly than conventionally‐reared birds. Satisfactory growth and efficiency of food utilisation were obtained by allowing these birds access to food during the dark periods.

3. Altering the lighting schedule to 2L:6D resulted in birds with food intakes and growth rates similar to those reared conventionally, even when feeding was restricted to the light periods.

4. Releasing birds from the light and food restriction for the 2 weeks preceding slaughter resulted in greater food consumption which was not characterised by significantly better growth or food utilisation.

5. Feeding a diet of high energy or pelleting a diet of low energy during the period of restriction increased food intake and improved growth rate.

6. The response of broilers to intermittent lighting and feeding was not significantly influenced by ambient temperature within the range 16 to 35 °C or by the energy concentration of the diet fed before the introduction of the intermittent schedule.     

Effect of dietary energy concentration on the response of laying hens to amino acids

1. A hypothesis, that the optimum amino acid concentration in the diet is not directly proportional to the dietary energy concentration, but changes in inverse proportion to the change in food intake resulting from a change in energy concentration, was tested in three experiments.

2. Response experiments involving the amino acids methionine, lysine and isoleucine were conducted, in each case at three dietary energy concentrations, using a diet dilution and blending technique, thereby ensuring a constant ratio between background amino acids and the first‐limiting amino acid in all diets, and also keeping the ratio of amino acids to energy constant as energy varied.

3. A common response curve relating egg output (g/bird d) to amino acid intake (mg/bird d) for each amino acid, fitted by means of the Reading Model, adequately described the response at each of the dietary energy contents. This implies that energy does not influence egg output directly, but only indirectly through its effect on food intake and hence on amino acid intake.

4. Both amino acid and energy concentration significantly influenced food intake. Energy intake was not constant over all dietary energy concentrations, being lower at low energy levels and higher at high energy concentrations.

5. It is concluded that amino acid requirements should not be stated either as percentages or as ratios with energy. Optimum amino acid intakes and energy concentrations should be calculated; the expected food intake should then be predicted, after which the appropriate concentration of nutrients in the diet can be determined.     

Behavioural and growth responses of broiler chickens to dietary water content and climatic variables

1. The behavioural responses and growth of broiler chickens were investigated over three seasons using two wet mashes with food:water ratios of 2:1 (HW) and 3:1 (MW).

2. Wet mashes fed in the hot dry season caused significantly lower rates of panting, a major thermoregulatory behaviour. This may be an indication of increased comfort in hot dry conditions for birds fed wet mashes.

3. Feeding activities of birds fed dry and wet rations respectively increased and decreased with decrease in seasonal temperature. The peaks of both feeding activity and consumption in the cool‐dry season were attained by birds fed dry diets and in the hot dry season by those fed wet mashes.

4. Diurnal distributions of food and water intakes were more even in the hot dry season for those fed wet mashes. This may again suggest a higher degree of comfort on the hot dry season when birds are fed wet mashes.

5. In the hot dry season, 60% of birds preferred wet mashes, whereas 80% of birds preferred dry meal in the cool dry season. This may be attributable to the lower temperature of wet mashes.

6. Similar patterns in feeding activity, food consumption and daily weight gain occurred in response to dietary presentation and season.

7. Feeding of dry meal under cool dry conditions and wet mashes in the hot season may be advantageous. Irrespective of season, water temperature may have to be raised to about 30°C before addition to meals for chicks under brooding. This may be necessary as preliminary investigations in this laboratory indicate that food temperatures below 22°C may at the brooding stage discourage food intake and activities.     

A comparison of the energy and nitrogen metabolism of fed ducklings and chickens

1. Energy measurements were made over 4 d on groups of three ducklings (aged from 5 to 22 d), and three broiler chickens (aged from 11 to 32 d) offered high‐ or low‐energy diets.

2. Food, metabolisable energy (ME) and water intakes were significantly higher for ducklings than for chickens. The ratio of water : food was 4.2 : 1 and 2.3 : 1 for ducklings and chickens, respectively. The food conversion ratio differed between diets but not species. Performance was generally better for both species on the high‐energy diet.

3. Heat production, energy, fat and protein retentions were higher for ducklings than chickens, and ducklings retained 0.44 of their energy as fat compared with 0.37 for chickens. Overall the ratio of protein (g) to fat (g) retention was 2.2 : 1 and 2.8 : 1 for ducklings and chickens respectively.

4. For ducklings, metabolisability of the high‐energy diet declined from 0.774 to 0.747, and to a lesser extent of the low‐energy diet, as they aged. There was no such decline for chickens. Net efficiency of utilisation of ME for gain was 0.64 for ducklings compared with 0.50 for chickens.

5. Fractional retention of dietary nitrogen (N) was 0.62 for ducklings and 0.55 for chickens. Gaseous ammonia‐N was 4.5 and 2.2%, respectively, of N retained.

6. In a second experiment groups of ducklings only, were offered high‐and low‐protein diets from 12 to 22 d of age. Comparisons among four diets showed that food and energy intake was lower on the low‐protein diet than on the other three. Energy retention on the high‐energy diet was greater (P<0.05) than on the other three diets.

7. It was concluded that a high‐energy diet is important for ducklings and chickens for maximum biological performance during the first 4 weeks of life.