机器人倾倒碰撞动力学建模与不同刚度下碰撞响应

为了确定刚度对柔性臂倾倒碰撞力的影响,基于混合坐标法建立柔性臂运动学模型,利用假设模态法对柔性变形解耦,基于Hertz接触理论和非线性阻尼理论建立接触碰撞力模型,利用Lagrange方程建立了含碰撞的动力学方程。编写了变步长变精度的四阶Runge-Kutta数值求解算法。对不同刚度下柔性臂的碰撞响应进行仿真,得到了接触力、关节转角、角速度和弹性变形曲线。对比可得,随着抗弯刚度增大,接触力变大且峰值出现的相位提前,且碰撞后柔性臂关节转角变小,弹性变形和角速度的振动都减小;材质结构阻尼对弹性振动变形有明显的抑制。该文所建模型和求解方法有效。     

椭圆齿轮行星系分插机构推秧装置的动力学模型的建立及验证

为了建立高速水稻插秧机椭圆齿轮行星系分插机构的动力学模型，必须分析推秧装置对该机构的作用。该文采用作者推导的解析形式刚体复合运动微分方程对该机构的推秧装置进行动力学分析，推导出推秧装置的动力学模型。通过编程计算得到：①推秧和碰撞过程行星架转角与拨叉转角及推秧杆位置的关系，并由高速录像验证；②在推秧和碰撞过程拨叉转动中心受力、推秧杆对栽植臂作用力和弹簧作用力与行星架转角的关系，为分析整个分插机构的动力学特性奠定基础。     

五自由度混联机器人优化设计与运动学分析

为提高农业自动化程度,拓宽并联机构在农业工程领域的应用,提出一种存在连续转轴、关节数目少、易于控制的两移一转运动冗余平面并联机构,该并联机构任意位置的转轴均为相互平行的连续转轴,使其具备良好的灵活性。基于此平面并联机构,构造出了多种五自由度混联机器人,首先建立了五自由度混联机器人的运动学模型,并对其进行了奇异分析,给出了减少机构奇异位型的条件;然后基于灵活性指标,对并联机构进行了尺寸优化,绘制了用于选取结构尺寸的性能图谱,且借助有限元软件对基于优化所得结构尺寸绘制的具有运动冗余特性的平面机构进行了结构拓扑优化,,完成了整体结构优化前后的静力学分析与对比,结果显示优化前后整体变形仅增大0.51%,优化前后机构优化部分的质量减少33.02%,满足机构变形要求。该混联机器人具有结构简单、运动学模型简单、结构刚度高和模块化程度高的特点,且其结构的变胞性有助于实现机构运动和驱动冗余模式的切换,增强了机器人的可研究性。该文可为混联机器人运动学分析及优化设计提供参考。     

气动侧摆关节的动态特性

针对目前气动关节存在的缺点,提出了采用气动柔性驱动器(flexible pneumatic actuator,FPA)直接驱动,模拟人手指侧摆运动的侧摆关节。介绍了侧摆关节的工作原理。根据热力学第一定律,结合关节的动力学方程,推导建立了关节的转角及输出力矩的动态模型,并进行了仿真分析研究了关节的动态特性;试验研究了侧摆关节的动态特性,分析了关节转角及输出力矩的实际动态响应较慢原因;采用了串联双闭环控制方法,对关节的转角及输出力矩进行了控制研究,结果表明:期望角度为15°时,关节转角闭环动态响应时间约为0.3s,稳态相对偏差小于0.65%;期望输出力矩为188N?mm时,闭环输出力矩动态响应约为0.3s,稳态相对偏差小于1.5%。侧摆关节可控性高,可满足多指灵巧手关节设计要求。     

果园巡检机器人长臂抖动抑制方法

针对果园巡检机器人长臂因抖动而严重制约图像采集效率的问题,该研究提出基于臂体上3个部位的仰角构建单一反馈量进行抖动抑制的方法。首先基于臂体外伸段的有限元模态分析结果,通过固有频率和静变形的等效,建立三杆两扭簧等效机构的动力学模型。并基于微分平坦理论将3个倾角传感器读数合成为一个系统输出量,以平坦输出量作为被控量,采用PID控制器进行抑振试验,再利用自抗扰控制(Active Disturbance Rejection Control, ADRC)思想中对广义扰动的处理方法,将被控模型等效为二阶系统形式,并将模型信息代入到扩张状态观测器中进行对比试验。试验结果表明,基于本文构建的方法均能起到快速抑制长臂抖动的作用。在冲击作用致使末端产生10°幅值的抖动情况下,PID控制器实现了7 s内抑制大幅抖动,自抗扰控制器实现了8 s内快速抑制大幅抖动的效果。PID控制器的输出出现3次饱和现象且小幅较高频率抖动不易消除,而自抗扰控制器仅出现一次饱和,具有抖动抑制过程平滑的优点。本文的控制算法简洁适于在单片机等微型计算机系统中应用,系统结构简单成本低,可为现代农业装备研发与应用中解决长臂类结构抖动的主动抑制问题提供理论参考。     

农用柔性底盘偏置轴转向机构联动耦合控制策略及试验

针对农用柔性底盘前轮转向时两偏置轴转向机构难以保持联动关系而影响顺利转弯的问题,基于阿克曼转向几何与交叉耦合控制原理,设计了偏置轴转向机构联动耦合控制策略,采用模糊PID控制算法对两转向轮转角联动轮廓误差进行补偿,并依据方向盘信号大小和变化率对电磁摩擦锁PWM控制信号占空比进行调节,以匹配偏置电动轮转向的角速度,使两转向机构形成耦合而保持期望联动关系;基于MATLAB/Simulink对控制策略进行了仿真,且在硬化路面上实施了阶跃转向、蛇行转向及随机转向3种运动方式的试验验证,并对比分析了转角分配控制下的前轮转向效果。试验结果表明:耦合控制方法下柔性底盘前轮阶跃转向响应均在0.8 s内,左、右侧转角最大超调为1.3°;电磁摩擦锁的开闭可较好匹配电动轮的转向;左、右前轮对于各自目标角具有良好的跟踪性能;3种转向方式下最大与平均跟随误差值均小于分配控制方法;两轮联动的最大与平均转角轮廓误差分别为:阶跃转向1.2°与0.6°、蛇行转向1.1°与0.6°、随机转向1.0°与0.5°;耦合控制下仿真与试验转角的轮廓误差变化趋势一致,最大误差为2.2°,证明仿真模型合理有效。耦合控制下偏置轴转向机构联动控制效果优于转角分配控制,转向效果良好,该文提出的柔性底盘偏置轴转向机构联动耦合控制策略有效且可行。     

立体苗盘管理机器人的机械臂参数优化与试验

为使立体苗盘管理机器人的机械臂能够在植物工厂狭窄的作业环境下,灵活、高效地完成目标工作空间的所有搬运和喷洒动作任务需求,同时尽量减小机械臂的操纵空间和结构尺寸,采用理论与试验相结合的方法对机械臂参数进行了优化设计。首先采用D-H法建立了机器人的运动学模型,然后通过工作空间分析确定出优化参数的工作空间约束条件。在此基础上,以"距离最短"和"结构紧凑"为性能指标建立目标优化函数,并利用遗传算法求解出最优的大臂杆长648 mm、中臂杆长472 mm和小臂杆长396 mm,最优机械臂关节转角极限值为96°、68°和126°。最后进行机器人样机的搬运和喷洒运动规划试验,并借助高速摄像系统标记机械臂末端运动轨迹坐标。试验结果表明:优化后的机械臂能够到达目标工作空间的所有极限位置及其他特征位置点,最大绝对定位误差为9.8 mm,最大相对定位误差为0.98%,在允许的误差范围内,能够满足机械臂工作空间对目标工作空间的有效包容。     

轴颈倾斜时滚柱轴承动态接触特性的有限元分析

针对滚柱轴承工作时存在轴颈倾斜的情况,利用ANSYS/LS-DYNA有限元分析软件开展不同轴颈倾斜角下滚动轴承动态接触特性的数值模拟研究,通过对比分析接触部位应力值和变形的差别,考察倾斜角大小对轴承动态接触特性影响情况。结果表明,考虑轴颈倾斜时滚子组最大等效应力、最大剪切应力仿真结果明显大于非倾斜状态下得到的结果,在倾斜角为0°、0.1°和0.5°等3种情况下,滚子组等效应力分别为700MPa、2.73GPa和4.0GPa;同时,滚子端面单元应力值和接触部位应力分布等仿真结果均随倾角增大而差异更加明显;但轴心在径向载荷方向的位移受倾斜角的影响不明显,均分布在-0.2～0.3mm范围内。研究结论可以为实际工作过程中考虑轴颈倾斜时滚柱轴承动态特性的研究和轴承结构优化设计提供参考。     

CMJY-1500型农田残膜捡拾打包联合作业机设计与试验

为降低残膜储运成本,提高机收残膜回收利用率,研究设计了CMJY-1500型农田残膜捡拾打包联合作业机。机具主要由捡拾机构、脱膜机构、打包机构、传动系统、地轮、机架等组成,可同时完成集条残膜的自动捡拾、杂质分离与打包成型作业。根据捡拾机构结构特征与作业原理,建立捡拾机构与物料对象动力学模型,分析获得了核心弹齿最佳空间排布形式与优选安装倾角45°。为降低残膜物料土壤掺杂量,基于残膜与杂质尺寸密度及缠绕特性差异,开发了离心分离与振动分离相结合的多级膜土分离系统,提高了捡拾物料压缩比,减小打包作业压缩阻力。设计了液压驱动式电控打包机构,残膜经打包机构压缩成型,以方包形式卸料,利于装载运输。2015年10月于兵团第六师共青团农场,对残膜捡拾打包机进行了40 hm~2实地田间作业性能测试,试验结果表明:机具残膜捡拾净率达92.8%,打包成捆率高于94%,单包成型耗时58 s,残膜捡拾打包联合作业机作业效果良好的、系统稳定,具有较高的应用推广价值。     

含关节间隙的Delta机器人弹性动力学与振动特性分析

针对经济实用型并联机器人关节间隙对动平台位置精度与系统振动特性影响的问题,以Delta机器人为研究对象,利用数理统计原理对含关节间隙的Delta机器人支链进行了运动学分析,结合Lankarani-Nikravesh碰撞接触力模型与具有动态修正系数的Coulomb摩擦模型对关节间隙广义碰撞力进行了研究。利用空间有限元理论与拉格朗日方程,充分考虑主、从动臂的空间动力特性与运动协调关系,建立了Delta机器人弹性动力学模型,在定义杆件虚长度的基础上,将关节间隙产生的广义碰撞力结合到弹性动力学模型中,建立了含关节间隙的Delta机器人弹性动力学模型。借助FARO激光跟踪仪对间隙弹性动力学模型进行了验证分析,利用脉冲锤击法与Workbench软件仿真对Delta机器人的振动特性进行了研究分析。试验结果表明,考虑关节间隙时动平台中心点的运动轨迹较不考虑关节间隙时更靠近试验运行结果,验证了间隙弹性动力学模型的合理性与正确性,并且,系统前两阶非零固有频率的理论值与试验值的相对误差分别为3.544%和12.026%,两者相当接近。另外,由仿真结果可以发现3组从动臂是Delta机器人整机系统中最薄弱的环节。该研究可为经济实用型并联机器人的位置误差补偿与系统减振优化提供参考。     

低氮和干旱胁迫对富士和秦冠生长及氮素利用的影响

【目的】以富士（Fuji）、 秦冠（Qinguan）嫁接在平邑甜茶（Malus hupehensis Rehd.）上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水（ZZ）、 低氮正常水（DZ）、 正常氮干旱（ZG）、 低氮干旱（DG）。氮素和水分均设置两个水平,分别为正常氮（6 mmol/L NO-3-N）、 低氮（0.3 mmol/LNO-3-N）、 正常供水（保持盆中砂子相对含水量为饱和含水量的80%~85%）、 干旱处理（保持盆中砂子相对含水量为饱和含水量的60%~65%）。【结果】富士和秦冠的生物量（茎和叶）、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）,并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）; 氮、 钾元素含量在植株各部位的分布顺序依次是叶＞根＞茎,磷元素则是根＞叶＞茎;光合氮素利用效率（PNUE）和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水（DZ）处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱（DG）处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。     

Pasture Vegetation Elemental Analysis by Laser-Induced Breakdown Spectroscopy

Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing.     

Faculty positions:Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Macroinvertebrates in North American tallgrass prairie soils: effects of fire, mowing, and fertilization on density and biomass

The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils.     

Variation in quality parameters between and within 14 Nordic tree fruit and berry species

Abstract

A 3-year study was carried out to investigate quality parameters in 14 tree fruit and berry species grown in southern Norway. The species were blueberry, apple, aronia, sour cherry, sweet cherry, red raspberry, strawberry, blackcurrant, gooseberry, red currant and elderberry, harvested along with wild bilberry, cloudberry and lingonberry. Significant differences between species were identified for all quality parameters. The coefficient of variation between species was lowest for pH (12.5%), dry matter (18.9%) and soluble solids (25.3%), followed by titratable acids (59.3%), total phenolics (83.8%), antioxidant capacity FRAP (85.7%) and antiradical power by the DPPH-assay (97.8%), total monomeric anthocyanins (132%) and ascorbic acid (137%). Average coefficient of variation within species were lower and ranged from 4 (pH) to 62% (ascorbic acid). Only the FRAP values were significantly affected by harvesting year with lower levels in 2004 than in 2005 and 2006. There were significant interactions between species and harvesting year for dry matter, soluble solids, pH, ascorbic acid and FRAP. The results indicate generic ranges in composition within species independent upon growing location and climate, and the composition of the tree fruits and berries is not likely to deviate from these ranges. It is concluded that desirable composition of tree fruits and berries and their products should primarily be achieved by selection among species rather than searching fors broadened variation within individual species.     

Potassium Fixation and Release Characteristics in Rhizosphere and Nonrhizosphere Soils for a Rapeseed–Rice Cropping Sequence

Potassium (K) fixation and release in soil are important factors in the long-term sustainability of a cropping system. Changes in K concentration and characteristics of K fixation and release in rhizosphere and nonrhizosphere soils in the rapeseed (Brassica napus L.)–rice (Oryza sativa L.) rotation were investigated using a rhizobox system. The concentrations of different forms of K in both rhizosphere and nonrhizosphere soils decreased with plants compared to without plants, regardless of K fertilizer application. Potassium uptake by crops mainly came from the rhizosphere soil. In the treatment without K fertilizer (–K), the main form of K supplied by the soil to the crops was 1.0 mol L^?1 nitric acid (HNO₃) nonextractable K, followed by nonexchangeable K, and then exchangeable K. In the treatment with K fertilizer (+K), the main K forms supplied by the soil to the crops were exchangeable K and nonexchangeable K. The amount and rate of K fixation after one cycle of the rapeseed–rice rotation was greater in rhizosphere soil than in nonrhizosphere soil. The amount and rate of K fixation of soil in the +K treatment were significantly less than in the –K treatment. The cumulative amounts of K released with 1.0 mol L^?1 ammonium acetate (NH₄OAc) and 1.0 mol L^?1 HNO₃ extraction increased with the increasing numbers of extractions, but the K-releasing power of soil by successive extraction decreased gradually and finally became almost constant. The release of K was less in rhizosphere soil than in nonrhizosphere soil. The release of K in the +K treatment was similar to that in the –K treatment in rhizosphere soil, but the K release in nonrhizosphere soil was greater with the +K than the –K treatment. Overall, the information obtained in this study will be helpful in formulating more precise K fertilizer recommendations for certain soils.     

Electron donor and pH relationships for biologically enhanced dissolution of chlorinated solvent DNAPL in groundwater

Biologically enhanced dissolution offers a method to speed removal of chlorinated solvent dense non-aqueous-phase liquid (DNAPL) sources such as tetrachloroethene (PCE) and trichoroethene (TCE) from aquifers. Bioremediation is accomplished by adding an electron donor to the source zone where fermentation to intermediates leading to acetic acid and hydrogen results. The hydrogen and possibly acetic acid are used by dehalogenating bacteria to convert PCE and TCE to ethene and hydrochloric acid. Reductive dehalogenation is thus an acid forming process, and sufficient alkalinity must be present to maintain a near neutral pH. The bicarbonate alkalinity required to maintain pH above 6.5 is a function of the electron donor: 800 mg/L of bicarbonate alkalinity is sufficient to achieve about 1.2 mM TCE dechlorination with glucose, 1.7 mM with lactate, and a much higher 3.3 mM with formate. Laboratory studies indicate that in mixed culture, formate can be used as an electron donor for complete conversion to ethene, contrary to pure cultures studies indicating it cannot. Various strategies can be used to add electron donor to an aquifer for DNAPL dehalogenation while minimizing pH problems and excessive electron donor usage, including use of injection-extraction wells, dual recirculation wells, and nested injection-extraction wells.     

Biodiversity and ecogeography of wild Lactuca spp. in some European countries

Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions.     

Increase in auxiliary photoprotective photosynthetic pigments in wheat seedlings induced by Azospirillum brasilense

Inoculation of wheat seedlings with the plant growth-promoting bacterium Azospirillum brasilense Cd was immobilized in alginate microbeads and, without applying any stress, significantly increased the quantity of several photosynthetic pigments, such as chlorophyll a, chlorophyll b, and the auxiliary photoprotective pigments violaxanthin, zeaxanthin, antheroxanthin, lutein, neoxanthin, and β-carotene. This resulted in greener plants with no apparent visible stress. After monitoring the quantity of photosynthetic pigments for 4 weeks, we observed that inoculated plants had higher quantities of pigments in shoot and stem. The greatest difference in the quantity of all pigments between inoculated and noninoculated plants occurred in the first week of growth. Regardless of treatment, the quantity of pigments in stems was three to four times less than the quantity of these pigments in shoots. Application of Azospirillum, either as liquid inoculant or as alginate microbeads, did not alter the positive effect of the bacteria on pigment production or the positive response of the plants towards A. brasilense Cd inoculation.     

In-situ enrichment and analysis of atrazine-degrading microbial communities using atrazine-containing porous beads

We examined the community composition of microbes that colonized atrazine-containing beads buried in agricultural soils that differed in atrazine treatment history. Bacterial abundance was 5-40-fold greater in atrazine-fortified beads. In beads containing 20 mg atrazine kg⁻¹ buried in soil with a history of atrazine application (conditioned soil), the abundance of Actinobacteria increased approximately 80-fold whereas in control soil, Actinobacteria were enriched only 10-fold and the gamma-Proteobacteria and Planctomycetes increased by 60- and 25-fold, respectively. The gamma-Proteobacteria were enriched by 120- and 230-fold in beads containing 200 mg atrazine kg⁻¹ in conditioned and control soil, respectively. The results demonstrate that BioSep^® beads are a suitable matrix for recruiting a diverse subset of the bacterial community involved in atrazine degradation.