陆地棉(G.hirsutum L.)种子活力的研究

本文通过棉花种子活力与电导率、阳离子外渗、组织结构、凝集素和种子加工的关系研究表明,种子活力下降,阳离子外渗增加,其中主要是K~+。种子各部分对逆境条件的反应不同,越是活跃的组织越易丧失活力。高活力的种子也是高凝集素的种子,在多逆抗性的选择过程中,利用低温发芽是对种子高活力和高凝集素等抗性物质的选择。因此,可考虑用电导率、胚根和下胚轴的比值作为检验棉子活力的指标。并证明棉子经我国自行研制的泡沫酸脱绒机处理,能有效地提高播种品质。     

陆地棉(G. hirsutum L.) N-乙酰氨基葡萄糖转移酶基因(GhGnT)的克隆及耐盐性功能分析

在耐盐相关基因芯片基础上,我们筛选到盐诱导显著上调表达(Ratio>2)的N-乙酰氨基葡糖转移酶基因.选取耐盐材料中9806为材料,根据耐盐性抑制消减文库EST序列设计引物,利用RACE及RT-PCR技术获得该基因cDNA全长1970 by,命名为GhGnT.通过Blast比对,发现该基因与蓖麻乙酞氨基葡萄糖转移酶基因...     

陆地棉族系种质系与陆地棉品种间的杂种优势利用研究

采用ＮＣⅡ（ＮｏｒｔｈＣａｒｏｌｉｎａⅡ）交配设计选用６个陆地棉族系种质系材料和３个常规品种组配１８个Ｆ１，对杂种及亲本进行比较研究，研究陆地棉族系种质系在杂种优势中的利用价值。研究结果表明，产量杂种优势表现明显，皮棉产量优势最大，其余依次为子棉产量、铃数、单铃种子数、单铃重。纤维品质优势表现软弱。试验表明产量杂种优势主要来源是铃数、单铃种子数、铃重增加。研究还发现亲本间遗传距离与杂种优势关系不明显；特殊配合力效应与优势率显著相关。     

陆地棉(Gossypium hirsutum)光敏色素B基因(GhPHYB)克隆及其生物信息学分析

本研究依据已公布的雷蒙德氏棉基因组测序结果,采用同源克隆的方法,以陆地棉TM-1幼苗为材料,利用RT-PCR技术克隆得到陆地棉光敏色素B基因(Gh PHYB)的c DNA序列。结果显示:该基因ORF全长3 582 bp,编码1 194个氨基酸;通过与已知的拟南芥光敏色素B氨基酸序列比对及蛋白结构预测,发现该基因包含植物光敏色素完整的结构,与烟草、拟南芥、水稻、小麦和玉米的氨基酸序列相似性分别为87.2%、77.9%、74.5%、74.5%和54.6%。同时,本研究还构建了Gh PHYB基因的RNAi干涉载体用于转化棉花,并通过构建棉花PHYB RNAi突变体了解陆地棉PHYB基因对棉花株型、开花期、产量、棉纤维品质、抗逆性等方面的调控作用。研究结果可为该基因今后应用于棉花生产奠定基础。     

Agrobacterium-mediated Transformation and Regeneration by Direct Shoot Organogenesis in Cotton (G. hirsutum)

Genotype independent transformation andregeneration of Indian cotton(Gossypiumhirsutum L.)cultivar was standardized with Bt-Cry 1A(b)gene by Agrobacterium-mediation.Apical meristem of elite G.hirsutum cultivarLRK-516 and LRA 5166 were co-cultivated withA.tumefaciens LBA 4404 carrying synthetic Bt-     

陆地棉86-1（G.hirsutum L.)与辣根棉（G.armourianum Kearn.)种间杂种及其利用的研究

陆地棉品种86-1 G.hirsutum L.×辣根棉C.armourianum Kearn.F_1染色体2n=3x=39,平均染色体构型为14.051 12.45Ⅱ 0.01Ⅲ。F_1直接与陆地棉回交,得到的BC_1F_1是一株混倍体(mixploid),其染色体为54—67个,其中2n=65的整倍体(euploid)只占15.7％,非整倍体(aneuploid)占82.8％,平均2n=61,平均染色体构型为7.741 25.83Ⅱ 0.45Ⅲ 0.06Ⅳ 0V 0.01Ⅵ。又以BC_1F_1为母本,与陆地棉二次回交,BC_2F_1苗期夭折;而以陆地棉为母本、BC_1F_1为父本回交的BC_2F_1生长健壮,有些辣根棉性状继续传递。通过多年选育已获得一些结铃性强、中长绒纤维高品质、高衣分的新材料。     

Development and Use of Radiation-induced Chromosomal Translocations and Primary Monosomics of Cotton(G. hirsutum L. )

Over 200 disomie plants with translocations ofcotton were recovered as heterozygotes followingused by four types of treatments:combinedtreatment of seeds with colchicines and Y-rays,irradiation of seeds by fast and thermal neutronsand γ-irradiation of pollen.Numbers oftranslocations obtained were differed in M1, M2and M3 generation after irradiation and aftertreatment by different doses of irradiation.     

Genetic Engineering of Cotton (Gossypium hirsutum L. ) for Insect-resistance

In order to improve insect-resistance of cottonand cultivate new cotton varieties,tissue cultureand plant regeneration of cotton(Gossypiumhirsutum L.)were studied with Xinluzao 4,Xi550,Jizi 492,Hengwu 89-30,Han 93-2 and Jizi123.A system of cotton tissue culture for rapid     

不同陆地棉品种辐射效应研究初报

不同陆地棉品种辐射效应研究初报①Ｓｔｕｄｉｅｓｏｎｒａｄｉａｔｉｏｎｅｆｅｃｔｏｆｄｉｆｅｒｅｎｔｃｏｔｏｎｖａｒｉｅｔｉｅｓ（Ｇ．ｈｉｒｓｕｔｕｍＬ．）试验以近几年育成的１０个综合性状较好的陆地棉品种（系）为材料，包括５个中熟品种：鲁棉９号（ｖ１）...     

牧草引种试验研究初报

随着西部大开发退耕还林还草、天然草原保护与建设、风沙治理、水土保持等生态环境保护与建设工程的具体实施,牧草和畜牧业得到迅速发展,牧草的需求也越来越大。新疆作为畜牧业的重要基地之一,适宜本区种植的优良牧草较少,品种单一等问题     

Construction of Molecular Linkage Map of Cultivated Allotetraploid Cotton (Gossypium hirsutum L.×G. barbadense L. )with SSR and RAPD Markers

A permanent doubled haploid population fromthe crossing of G.hirsutum × G.barbadensewere developed by means of Vsg,virescentlymarked semigamy line in sea island cotton,which was characterized by a cytologicalmechanism for developing haploids with certainconvenience,and thus constructed an     

啤酒花国外引种研究初报

本试验对来自日本的7个啤酒花品种,从成熟期、丰产性及品质3个方面综合分析,比较研究,选择适于新疆栽培的优良品种。结果表明:麒麟丰绿的产量和α酸含量最佳,适合新疆种植;麒麟二号、甲斐黄金和K-52,可视实际情况适量发展;K-51和K-53的品质优、产量低,值得进一步探讨;C-345有异味,不宜种植。     

北京郊区紫花苜蓿引种研究初报

研究表明,紫花苜蓿能够在京郊种植,播种期以４月份为宜,从初蕾期至中花期,鲜,干草产量和营养品质均处于较理想状态,此段时间刈割最佳。     

我国陆地棉品种的遗传多样性研究初报

选用１８个随机引物，对２１个陆地棉品种作ＲＡＰＤ分析，并对各品种的指纹图谱进行了聚类和相似性分析。结果表明大部分品种与其系谱吻合。研究结果充分展示了ＲＡＰＤ技术可以作为棉花品种分类和遗传多样性研究的可行方法。     

Genetics, Genetic Collection of Characters of Structural Shrub Characteristics of G. hirsutum L.

The structure and shape of a cotton shrub aredetermined in result of combining theirelements:length and amount internodes of amain stem and fruit brunches,amount and shapeof leaf blades.Some lines of genetic collectionhave been crossed between each other for many     

Heredity, Reproductive and Adaptive Heterosis and Variability of Intraspecies Hybrids of G. hirsutum L.

On the patterns of geographically remote G.hirsutum L.cotton hybrids produced byintraspecies hybridization of local and importedbreeds we have investigated particularities inheredity and variability of the most importanteconomical and physiological features,display ofreproductive and adaptive heterosis in different     

Development of SSR Markers towards Genetic Mapping in Cotton(Gossypium hirsutum L. )

Availability of informative molecular markers isa prerequisite for genetic mapping and marker-assisted selection projects.Micro-satellites orSimple Sequence Repeat(SSR)markers arePCR-based and currently the most widely usedmarker system in the plant molecular geneticscommunity due to their high degree ofpolymorphism,random distribution throughoutthe genome and their suitability for highthroughput genotyping formats.Despite itsglobal economic importance,cotton has slower     

日本香稻引种试验研究初报

引进的13个日本香稻品种中，香味、株型结构及产量性状表现均较好的品种为“冷”，其次是“十和锦”和“祝贺”。具有良好株型结构的“Qiu香”作为清香型香稻品种，也有较好的利用价值。     

Identification of DNA Markers for Cotton Leaf Curl Disease (CLCD) in Cotton (Gossypium hirsutum L. )

Epidemic of cotton leaf curl virus disease(CLCD)was the compelling factor to devise newstrategies in cotton breeding programs ofPakistan.The evaluation of cotton genotypesagainst the CLCD resistance is difficult,expensive and time consuming in field andespecially in greenhouse due to unevendistribution of the disease.A marker-assisted