长期定位施肥对潮土有机氮组分和有机碳的影响

利用河南封丘潮土的13年长期施肥试验,采用Bremner法研究了潮土耕层有机氮组成的变化,分析了长期施肥对土壤有机氮组份和有机碳含量的影响。与不施肥和单施化肥相比,施有机肥或有机肥与化肥配施显著提高了土壤全氮、酸解有机氮、酸解铵态氮、氨基酸态氮、非酸解有机氮和有机碳的含量。有机氮主要由酸解铵态氮和氨基酸态氮组成,其次为酸解未知态氮和非酸解有机氮,氨基糖态氮含量最小。施有机肥尤其利于氨基酸态氮和非酸解有机氮的形成。施肥处理的酸解有机氮占全氮的比例减小,主要是由氨基酸态氮、酸解铵态氮占全氮的比例减小所致。与1989年试验开始时的土壤初始值相比,施有机肥能提高土壤全氮含量和有机质含量。在供应等氮磷钾的情况下,有机无机配施增加了土壤供氮能力、有机质含量和作物产量,是维持土壤肥力和保护环境最优的施肥方式。     

The contribution of soil organic matter fractions to carbon and nitrogen mineralization and microbial community size and structure

The aims of this study were to: (i) assess the impact of hay and fertilizer application on organic matter (OM) fractions (dissolved organic matter (DOM), light fraction organic matter (LFOM, <1.0 g cm⁻³), heavy fraction OM (HFOM, <1.7 g cm⁻³)), carbon (C) and nitrogen (N) cycling processes and microbial community size and structure, and (ii) quantify the role of OM fractions to C and N cycling. Soil was collected in 2001 from a field experiment to which grass hay (1996) and/or fertilizer (1995 and 1999) had previously been applied. DOM-C (P<0.05) and DOM-N (P=0.07) were significantly higher in control and fertilized soil than hay and hay+fertilized soil. LFOM and HFOM C and N contents and C/N ratios were significantly (P<0.05) higher in hay+fertilized and hay amended soil than in control and fertilized soil. Potentially mineralizable-N (PMN), microbial biomass-C (MB-C), microbial biomass-N (MB-N) and microbial respiration (CO₂) were not affected by fertilizer and/or hay application. Gross N mineralization (Gross Min) and gross nitrification (Gross Nit) rates were significantly (P<0.05) higher in fertilized, hay, hay+fertilized soil than control soil. However, there was no significant difference between treatments in gross N immobilization rates. Results reported here highlight the importance of a labile fraction of the DOM pool to N and C cycling as its removal significantly (P<0.05) reduced PMN, MB-N, Gross Min and Gross Nit compared with whole soil in most or all treatments. In soil where DOM+LFOM were removed PMN was significantly (P<0.05) lower, but MB-C, Gross Min and Gross Nit was significantly (P<0.05) higher than in DOM removed soil. This suggests that LFOM plays an important role as a sink for mineral-N. Total soil phospholipid fatty acid (PLFA) concentration was significantly (P<0.05) higher in hay amended than control, fertilized and hay+fertilized soil. Principal components analysis was able to clearly discriminate between control, fertilized, hay+fertilized and hay amended soil. Soil amended with hay or fertilizer had a microbial community structure which differed from that of the control or hay+fertilized soils. Redundancy analysis with Monte Carlo permutation tests revealed that PLFA profiles were strongly correlated to differences in Gross Min, Gross Nit, MB-N, MB-C, MB-C/N ratio, total soil C and total soil C/N ratio. The results of this research suggest that changes in microbial structure are related to aspects of soil C and N pools and cycling.     

Input related microbial carbon dynamic of soil organic matter in particle size fractions

This paper investigated the flow of carbon into different groups of soil microorganisms isolated from different particle size fractions. Two agricultural sites of contrasting organic matter input were compared. Both soils had been submitted to vegetation change from C3 (Rye/Wheat) to C4 (Maize) plants, 25 and 45 years ago. Soil carbon was separated into one fast-degrading particulate organic matter fraction (POM) and one slow-degrading organo-mineral fraction (OMF). The structure of the soil microbial community were investigated using phospholipid fatty acids (PLFA), and turnover of single PLFAs was calculated from the changes in their ¹³C content. Soil enzyme activities involved in the degradation of carbohydrates was determined using fluorogenic MUF (methyl-umbelliferryl phosphate) substrates.We found that fresh organic matter input drives soil organic matter dynamic. Higher annual input of fresh organic matter resulted in a higher amount of fungal biomass in the POM-fraction and shorter mean residence times. Fungal activity therefore seems essential for the decomposition and incorporation of organic matter input into the soil. As a consequence, limited litter input changed especially the fungal community favoring arbuscular mycorrhizal fungi. Altogether, supply and availability of fresh plant carbon changed the distribution of microbial biomass, the microbial community structure and enzyme activities and resulted in different priming of soil organic matter.Most interestingly we found that only at low input the OMF fraction had significantly higher calculated MRT for Gram-positive and Gram-negative bacteria suggesting high recycling of soil carbon or the use of other carbon sources. But on average all microbial groups had nearly similar carbon uptake rates in all fractions and both soils, which contrasted the turnover times of bulk carbon. Hereby the microbial carbon turnover was always faster than the soil organic carbon turnover and higher carbon input reduced the carbon storage efficiency from 51% in the low input to 20%. These findings suggest that microbial community preferentially assimilated fresh carbon sources but also used recycled existing soil carbon. However, the priming rate was drastically reduced under carbon limitation. In consequence at high carbon availability more carbon was respired to activate the existing soil carbon (priming) whereas at low carbon availability new soil carbon was formed at higher efficiencies.     

长期施用有机肥对稻麦轮作体系土壤有机碳氮组分的影响

【目的】 以湖北武汉地区长期稻麦轮作制度下施肥试验地作为研究对象,研究了长期不同施肥处理对耕层土壤有机碳、全氮及活性碳氮组分的影响,为优化稻麦轮作体系下施肥措施,实现土壤固碳减排,培肥土壤提供理论依据。 【方法】 长期施肥试验开始于1981年,试验处理包括不施肥 (CK)、施化学氮肥 (N)、施化学氮磷肥 (NP)、施化学氮磷钾肥 (NPK)、单施有机肥 (M) 及有机无机肥配施处理 (NPKM)。收集2017年小麦收获后耕层 (0—20 cm) 土壤,测定各小区土壤中的有机碳 (SOC)、全氮 (TN)、微生物量碳氮 (MBC、MBN)、水溶性碳 (DOC)、热水溶性有机碳 (HWSC)、颗粒有机碳氮 (POC和PON)、轻组有机碳氮 (LFOC和LFON) 及氯化钾浸提氮 (KEN,即水溶性无机氮) 的含量并分析各指标间的关系。 【结果】 1) 除KEN外,长期施用有机肥显著增加耕层土壤的各碳氮组分含量,特别是有机无机肥配施处理。2) 各活性有机碳组分占SOC的百分比由高到低排序为POC > LFOC > HWSC > MBC > DOC,各氮组分占TN的百分比由高到低排序为PON > LFON > MBN > KEN,其中POC占SOC的24.04%～37.64%,PON占TN的12.09%～20.24%,且有机肥处理下POC/SOC、PON/TN显著高于其余处理。3) 通过对土壤有机碳及各活性有机碳的对施肥的敏感性分析可得,各活性碳敏感性指数均显著高于SOC,且DOC的敏感性最高。4) 通过各组分间相关性分析可知,除KEN外,各碳、氮组分间显著正相关,其中DOC与SOC、PON与TN关系更为紧密,表明DOC及PON可较好地反应出SOC、TN的变化情况。 【结论】 在湖北稻麦轮作地区,长期有机无机肥配施处理显著增加了土壤碳库及氮库,促进了土壤碳、氮的积累,尤其是颗粒有机碳和有机氮 (POC和PON)。水溶性碳 (DOC) 对施肥反应最为敏感,可作为指示该地区有机物早期变化的指示物。      

Gaseous carbon emission in relation to soil carbon fractions and microbial diversities as affected by organic amendments in tropical rice soil

The interactive effects of moisture and organic amendments (farmyard manure (FYM), crop residue (CR) and green manure (GM) (Sesbania aculeata) on gaseous carbon (C) emission, soil labile C fractions, enzymatic activities and microbial diversity in tropical, flooded rice soil were investigated. The amendments were applied on equal C basis in two moisture regimes, that is, aerobic and submergence conditions. The CO₂ production was significantly higher by 22% in aerobic than in submergence condition; on the contrary, the CH₄ production was 27% higher under submergence condition. The labile C fractions were significantly higher in GM by 26% under aerobic and 30% under submergence conditions, respectively, than control (without any kind of fertilizer or amendments). Eubacterial diversity identified by PCR-DGGE method (polymerase chain reaction coupled with denaturant gradient gel electrophoresis) was higher under GM followed by FYM, CR, and control and it is pronounced in submerged condition. GM favored the labile C accumulation and biological activities under both submergence and aerobic conditions, which makes it most active for soil–plant interactions compared to other organic amendments. Considering environmental sustainability, the use of GM is the better adoptable option, which could enhance labile C pools, microbial diversities in soil and keep soil biologically more active.     

The role of amino acids and nucleic bases in turnover of nitrogen and carbon in soil humic fractions

Incorporation of labelled ¹⁵N and ¹⁴C amino acids and nucleic bases into soil humus fractions as well as humus turnover was investigated under field conditions. The dynamics of ¹⁵N and ¹⁴C incorporation into organic matter was characterized by the following main steps: rapid incorporation of the labelled substance prevailing for the first 1–3 weeks, and decomposition of included labelled fragments prevailing beyond one month after substance addition. The annual turnover rates of N and C in humus fractions due to incorporation of amino acids and nucleic bases were calculated. The turnover rate of N in humus is two to three times that of C. The contribution of amino acids to organic matter generation is about twice as great as that of nucleic bases and other N-containing organic substances. This indicates the important role of amino acids in the humification process and humus turnover. Turnovers of humic acids (0·002 year^?1 for C and 0·02 for N) are the most rapid of humic fractions investigated, and humin is characterized by the slowest turnover (0·0002 year^?1 for C and 0·007 for N). There are no significant differences in the turnover rates of fulvic acid fractions (0·0002 year^?1 for C) with different molecular weight.     

Protozoan predation and the turnover of soil organic carbon and nitrogen in the presence of plants

Summary The impact of protozoan grazing on the dynamics and mineralization of ¹⁴C- and ¹⁵N-labelled soil organic material was investigated in a microcosm experiment. Sterilized soil was planted with wheat and either inoculated with bacteria alone or with bacteria and protozoa or with bacteria and a 1:10 diluted protozoan inoculum. ¹⁴C–CO₂ formation was continuously monitored. It served as an indicator of microbial activity and the respiration of soil organic C. The activity of protozoa increased the turnover of ¹⁴C-labelled substrates compared to soil without protozoa. The accumulated ¹⁴C–CO₂ evolved from the soils with protozoa was 36% and 53% higher for a 1:10 and for a 1:1 protozoan inoculum, respectively. Protozoa reduced the number of bacteria by a factor of 2. In the presence of protozoa, N uptake by plants increased by 9% and 17% for a 1:10 and a 1:1 protozoan inoculum, respectively. Both plant dry matter production and shoot: root ratios were higher in the presence of protozoa. The constant ratio of ¹⁵N: ¹⁴⁺¹⁵N in the plants for all treatments indicated that in the presence of protozoa more soil organic matter was mineralized. Bacteria and protozoa responded very rapidly to the addition of water to the microcosms. The rewetting response in terms of the ¹⁴C–CO₂ respiration rate was significantly higher for 1 day in the absence and for 2 days in the presence of protozoa after the microcosms had been watered. It was concluded that protozoa improved the mineralization of N from soil organic matter by stimulating the turnover of bacterial biomass. Pulsed events like the addition of water seem to have a significant impact on the dynamics of food-chain reactions in soil in terms of C and N mineralization.Communication No. 19 of the Dutch Programme on Soil Ecology of Arable Farming Systems     

Influences of organic material application on the physically separated soil organic carbon and nitrogen fractions in rice fields

Purpose

The objectives of this study were to evaluate the effects of long-term organic materials incorporation on the soil aggregate and density-based fractions, and associated soil carbon (C) and nitrogen (N) conversion in the rice fields.

Materials and methods

A long-term located experiment was conducted to study the effects of continuous application of organic materials (milk vetch, rice straw, and poultry manure) on the distribution characteristics of soil aggregate and density-based fraction, as well as its organic C and N, in rice fields. The soil aggregate was classified using the wet-sieving method. Light fraction (LF) and heavy fraction (HF) were classified according to density fractionation. Aggregate organic C (AC) and total N (AN), LF organic C (LFC) and N (LFN), and HF organic C and N concentrations were measured by using the Elementar Vario ISOTOPE elemental analyzer.

Results and discussion

Application of organic materials increased the aggregate mass proportion of 2–0.25 mm (by 4.9–12.6%) and 0.25–0.053 mm (by 27.5–40.7%) fraction and its AC and AN concentration. The soil aggregate particulate organic C and total N were greatly improved with organic materials application. Furthermore, organic material had more obvious effect on the soil C and N in the LF than HF, which improved the LF particulate mass proportions by 75.1–177.0%, LFC by 51.7–68.4%, and LFN by 14.2–111.2%, respectively. Poultry manure had the greatest effect on increasing the AC, LFC, AN, and LFN, followed by milk vetch and rice straw.

Conclusions

Milk vetch, rice straw, and poultry manure could effectively increase the soil intermediate aggregate and LF proportion, and stimulate the stabilization and fixation of C and N in rice fields. It is an effective agricultural practice by applying organic material to improve soil fertility and sustaining high crop productivity. The increases of intermediate aggregate and associated C and N may be the main factor for soil C and N sequestration under continual application of organic materials.

     

玉米秸秆质量和土壤水分对土壤活性有机碳组分和微生物性质的影响

Investigating the effects of residue chemical composition on soil labile organic carbon(LOC) will improve our understanding of soil carbon sequestration.The effects of maize residue chemical composition and soil water content on soil LOC fractions and microbial properties were investigated in a laboratory incubation experiment.Maize shoot and root residues were incorporated into soil at 40%and 70% ?eld capacity.The soils were incubated at 20?C for 150 d and destructive sampling was conducted after 15,75,and 150 d.Respiration,dissolved organic carbon(DOC),hot-water extractable organic carbon(HEOC),and microbial biomass carbon(MBC)were recorded,along with cellulase and β-glucosidase activities and community-level physiological pro?les.The results showed that the cumulative respiration was lower in root-amended soils than in shoot-amended soils,indicating that root amendment may be bene?cial to C retention in soil.No signi?cant differences in the contents of DOC,HEOC and MBC,enzyme activities,and microbial functional diversity were observed between shoot- and root-amended soils.The high soil water content treatment signi?cantly increased the cumulative respiration,DOC and HEOC contents,and enzyme activities compared to the low soil water content treatment.However,the soil water content treatments had little in?uence on the MBC content and microbial functional diversity.There were signi?cantly positive correlations between LOC fractions and soil microbial properties.These results indicated that the chemical composition of maize residues had little in?uence on the DOC,HEOC,and MBC contents,enzyme activities,and microbial functional diversity,while soil water content could signi?cantly in?uence DOC and HEOC contents and enzyme activities.     

不同有机物料对苏打盐化土有机碳和活性碳组分的影响

【目的】在大同盆地苏打盐化土上,研究不同有机物料对春玉米产量、土壤有机碳及活性碳组分的影响,明确土壤有机碳及活性碳组分与主要盐碱指标的相关关系,为苏打盐化土改良及有机物料资源化利用提供理论支撑。【方法】2016-2017年在山西省北部怀仁县开展田间定位试验,设对照(CK)、风化煤、生物炭、牛粪和秸秆5个处理,各处理有机物料施用量按照每年9000 kg/hm^2等有机碳投入量折算,收获时对春玉米进行测产。2017年春玉米收获后,采集土壤样品测定土壤有机碳总量(SOC)和水溶性有机碳(WSOC)、易氧化有机碳(EOC)、轻组有机碳(LFOC)含量,分析土壤活性碳组分占有机碳的比例、土壤有机碳及活性碳组分与盐碱指标之间的关系。【结果】与CK相比,生物炭和秸秆处理春玉米产量无明显差异,而风化煤和牛粪处理春玉米产量则分别显著提高30.2%和30.3%。添加有机物料促进了0-20 cm土层SOC累积,其中以风化煤和牛粪处理效果最佳,较CK分别提高47.6%和36.1%。在有机碳组分方面,风化煤和牛粪处理提高WSOC、EOC含量的效果显著高于生物炭、秸秆处理;风化煤、牛粪和秸秆处理的LFOC含量显著高于生物炭处理。四类有机物料处理的WSOC占总有机碳的比例差异不显著,牛粪处理的占比显著高于CK。EOC占总有机碳的比例以牛粪处理最高,风化煤次之,且二者均显著高于CK处理;LFOC占总有机碳的比例则表现为秸秆、牛粪>风化煤、生物炭> CK。此外,添加有机物料能有效降低0-20 cm土层土壤pH、电导率(EC)和碱化度(ESP),其中以风化煤和牛粪处理降幅最大。相关分析表明,土壤SOC与pH、EC和ESP呈显著负相关。【结论】通过有机物料改良效果比较,发现牛粪和风化煤处理能促进苏打盐化土有机碳累积,提高可溶性、易氧化态及轻组有机碳组分在总有机碳中的占比,降低土壤pH、EC和ESP,明显提高春玉米产量。因此,风化煤和牛粪是山西北部苏打盐化土良好的改良剂。     

Quantifying soil organic carbon fractions by infrared-spectroscopy

Methods to quantify organic carbon (OC) in soil fractions of different stabilities often involve time-consuming physical and chemical treatments. The aim of the present study was to test a more rapid alternative, which is based on the spectroscopic analysis of bulk soils in the mid-infrared region (4000-400 cm⁻¹), combined with partial least-squares regression (PLS). One hundred eleven soil samples from arable and grassland sites across Switzerland were separated into fractions of dissolved OC, particulate organic matter (POM), sand and stable aggregates, silt and clay particles, and oxidation resistant OC. Measured contents of OC in each fraction were then correlated by PLS with infrared spectra to obtain prediction models. For every prediction model, 100 soil spectra were used in the PLS calibration and the residual 11 spectra for validation of the models. Correlation coefficients (r) between measured and PLS-predicted values ranged between 0.89 and 0.97 for OC in different fractions. By combining different fractions to one labile, one stabilized and one resistant fraction, predictions could even be improved (r=0.98, standard error of prediction=16%). Based on these statistical parameters, we conclude that mid-infrared spectroscopy in combination with PLS is an appropriate and very fast tool to quantify OC contents in different soil fractions.     

外源化肥氮素在土壤有机氮库中的转化及关系

【目的】本研究旨在探明外源化肥氮在土壤不同有机氮库中的动态转化及关系,为实现化肥氮素养分高效利用的有效调控提供理论依据。【方法】利用15N示踪技术(15N标记尿素,丰度10.3%),以江西红壤性水稻土为研究对象,通过土壤培养试验,研究农民习惯施肥水平下,水稻不同生育期外源化肥氮在土壤有机氮库(氨基酸态氮、 氨基糖态氮、 酸解铵态氮、 酸解未知氮和非酸解有机氮)中的转化及关系。采用通径分析方法,评估来自外源化肥氮的有机氮各组分之间的转化关系。【结果】 1)土壤中氨基酸态氮和氨基糖态氮中来自外源的化肥氮(氨基酸态氮-15N和氨基糖态氮-15N)含量从分蘖期到拔节期显著升高(P0.05),从拔节期到灌浆期显著降低(P0.05),全生育期两个组分中来自外源化肥氮的含量最高值分别为26.5和8.4 mg/kg,均出现在分蘖期和拔节期之间;酸解性铵态氮中来自外源的化肥氮(酸解性铵态氮-15N)含量从分蘖期到成熟期逐渐降低,全生育期的动态转化符合指数递减方程;酸解未知态氮中来自外源的化肥氮(酸解未知态氮-15N)含量随着生育期的延长逐渐达到动态平衡,最大值接近12.8 mg/kg;非酸解性有机氮中来自外源的化肥氮(非酸解性有机氮-15N)含量在全生育期的变化符合对称方程,最低值7.9 mg/kg出现在拔节期和灌浆期之间。2)在水稻营养生长阶段的分蘖期和拔节期,外源化肥氮分别以酸解性铵态氮和氨基酸态氮为主要方式结合到土壤有机氮库中,其含量分别占施入化肥氮的21.5%和14.8%;在水稻营养生长和生殖生长并进阶段(灌浆期)和生殖生长阶段(成熟期),外源化肥氮主要结合到非酸解性有机氮库中,分别占施入化肥氮的8.7%和12.7%。3)土壤各有机氮库中来自外源的化肥氮之间存在相互转化的关系,酸解性铵态氮库起到了“暂时库”的作用,生育前期在土壤中固持氮,当可利用性氮受限时,又可以作为有效氮库释放氮供作物吸收;在整个生长期中氨基酸态氮库对外源化肥氮的转化积累起到了“过渡库”的作用,固持在氨基酸中的化肥氮可以转化成酸解性铵态氮和氨基糖态氮。4)灌浆期和成熟期植物吸收的来自外源的化肥氮与氨基酸态氮-15N和酸解铵态氮-15N的关系更密切。【结论】外源化肥氮在土壤中转化的过程中酸解性铵态氮起到了“暂时库”的作用,氨基酸态氮起到了“过渡库”的作用,非酸解性有机氮可作为氮素的“稳定库”存在,外源氮在这几个主要的氮库中动态转换以保持土壤-作物体系中氮素的循环。     

生草对渭北旱地苹果园土壤有机碳组分及微生物的影响

在渭北旱地苹果园行间播种毛苕子（Vicia villosa）、白三叶（Trifolium repens）、黑麦草（Lolium perenne）和早熟禾（Poa pratensis）,以清耕为对照,对0100 cm土层的土壤有机碳各组分及微生物群落功能多样性进行研究。结果表明,行间生草可显著增加040 cm土层土壤的总有机碳（TOC）、颗粒有机碳（POC）、轻质有机碳（LFOC）、易氧化有机碳（ROC）、可溶性有机碳（DOC）和微生物量碳（MBC）,豆科牧草毛苕子和白三叶的各有机碳含量总体上高于禾本科牧草黑麦草和早熟禾。其中在020 cm土层中,豆科牧草的TOC含量平均每年增加约1.2 g/kg,禾本科牧草每年增加约0.9 g/kg。生草处理的微生物群落碳源利用率（AWCD）、微生物群落Shannon 指数（H）和微生物群落丰富度指数（S）均高于清耕处理,其中豆科牧草的微生物活性更高。因此,生草可以提高土壤有机碳各组分的含量、土壤微生物群落碳源利用率、微生物群落的丰富度和功能多样性,豆科牧草毛苕子和白三叶提高效应更加明显。     

Effect of biochar on soil structure and storage of soil organic carbon and nitrogen in the aggregate fractions of an Albic soil

ABSTRACT

Field experiment was conducted to evaluate the effect of corn straw derived-biochar (700 °C) applied at 0 (control), 10 (B1), 20 (B2) and 30 t ha^?1 (B3) on water stable aggregate (WSA), mean weight diameter (MWD), total organic carbon (TOC) and total nitrogen (TN) in WSA fractions of Albic soil. Compared with control, WSA in > 2 mm fraction increased, by 40.8% and 51.5% (0–10 cm depth) in B1 and B3, respectively. B1, B2 and B3 (10–20 cm depth) increased by 55.2%, 69.6% and 62.4%, respectively. MWD increased by 34.4%, 21.6%, and 17.6% with B3 at 0–10 cm, 10–20 cm and 20–30 cm depths, respectively. TOC in the > 2 mm fraction increased by 28.6%, 22.1%, and 23.2% (0–10 cm depth) in B1, B2, and B3, respectively, TN in 2–0.5 mm fractions increased by 32.4%, 23.4% and 33.6% (0–10 cm depth); and in the 0.25–0.05 mm fractions increased by 14.8%, 19.8% and 18.7% (10–20 cm depth), in B1, B2 and B3, respectively. Our findings suggest biochar application at 30 t ha^?1 could improve structural stability and sequestration of TOC and TN in Albic soils.     

Long-term manure application increased soil organic carbon and nitrogen mineralization through accumulation of unprotected and physically protected carbon fractions

Soil organic carbon(SOC) and nitrogen(N) mineralization are important biogeochemical processes associated with soil fertility. These processes are influenced by physically, chemically, and biologically stabilized SOC fractions, the mechanisms of which are not well known. The present study was conducted to evaluate the combined effect of manure and mineral fertilizers on the contents of SOC fractions to promote the mineralization of SOC and N.Treatments included: i) no fertilizer control(CK); ii)...     

长期撂荒对黑土土壤有机碳组分的影响

为明确长期撂荒对黑土不同土层土壤有机碳及其组分的影响,以吉林公主岭黑土长期定位试验为对象,选取不施肥和撂荒2个处理,采用改良的Walkley-Black土壤有机碳分组方法,研究不同层次(0～0.2、0.2～0.4和0.4～0.6 m)土壤有机碳及其组分的变化.结果表明:(1)0～0.6 m土壤剖面中,撂荒处理下土壤有机...     

Carbon input and crop-related changes in microbial biomarker levels strongly affect the turnover and composition of soil organic carbon

It is increasingly recognized that a detailed understanding of the impacts of land use on soil carbon pools and microbially mediated carbon dynamics is required in order to accurately describe terrestrial carbon budgets and improve soil carbon retention. Toward this understanding, we analyzed the levels of biomarkers including phospholipid fatty acids, amino acids, monosaccharides, amino sugars, and several indicators of labile and stabilized carbon in soil samples from a long-term agricultural field experiment. Our results imply that the composition of soil organic carbon (SOC) strongly depends on both the applied fertilization regime and the cultivated crop. In addition, our approach allowed us to identify possible mechanisms of microbial growth and contributions to soil carbon storage under different long-term agricultural management regimes.Amino acids and monosaccharides were quantitatively the most dominant biomarkers and their levels correlated strongly positively with microbial biomass. The relative contributions of the studied biomarkers to the total SOC varied only slightly among the treatments except in cases of extreme fertilization and without any fertilizer. In case of extreme fertilization and with alfalfa as crop type, we found evidence for accumulation of microbially derived monosaccharides and amino acids within the labile OC pool, probably resulting from soil C saturation. Interestingly, we also found an accumulation of microbially derived monosaccharides and amino acids in completely unfertilized plots, which we assumed to be caused by the smaller pore space volume and subsequent oxygen limitation for microbial growth. Mineral fertilization also had substantial effects on soil organic N when applied to plots containing alfalfa, a leguminous plant. Our results demonstrate that over-fertilization, fertilizer type, and the cultivated crop type can have major impacts on the turnover and composition of soil organic carbon, and should be considered when assessing management effects on soil C dynamics.     

Filtration increases the correlation between water extractable organic carbon and soil microbial activity

A study was carried out in order to establish the relationship between the water extractable organic carbon (WEOC) content of soils and soil microbial activity, and to determine how variations in the extraction procedure might influence the quantity of WEOC recovered. Concentrations of WEOC were determined in soils taken from 12 different sites in the south east of Scotland, using a procedure in which samples were shaken with distilled water, centrifuged at 5000g and then filtered through 0.45 μm Millipore filters. Filtration resulted in between 30 and 400 μg C g⁻¹ being extracted using this procedure and the concentration of WEOC in the resultant extracts correlated with soil microbial production of CO₂ and dehydrogenase activity (P<0.001). Without filtration, although more WEOC was extracted (between 31 and 716 μg C g⁻¹), there was no significant correlation with biological activity. There was also no correlation between WEOC and nitrous oxide release during the incubations. Centrifugation at 20,000g for at least 10 min prior to filtration was required to remove particulate organic materials. Storage of samples at 4 °C or for up to 1 week or freezing for up to 3 months was not found to have a large influence on the concentration of WEOC in extracts, although amounts increased with soil:extractant ratio and increasing extraction time (from 15 to 60 min).     

Effect of ultrasonic power on soil organic carbon fractions

Ultrasonic dispersion is a prevalent tool for soil fractionation. It is widely ignored that variation in ultrasonic power might lead to significantly different dispersion. We evaluated the effect of power variation with constant energy on the fine fraction mass, its organic C content and quality. All parameters increased significantly with power. The term “stable aggregates” as used in fractionation schemes cannot be defined by ultrasonic energy alone but power needs to be standardized, too.