Effects of excess aluminum on mineral uptake in mycorrhizal sorghum

Soil acidity is often associated with toxic aluminum (Al), and mineral uptake usually decreases in plants grown with excess Al. This study was conducted to evaluate the effects of Al (0, 35, 70, and 105 μM) on Al, phsophorus (P), sulfur (S), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), zinc (Zn,) and copper (Cu) uptake in shoots and roots of sorghum [Sorghum bicolor (L.) Moench, cv. SC283] colonized with the vesicular‐arbuscular mycorrhizal (VAM) fungi isolates Glomus intraradices UT143–2 (UT143) and Glomus etunicatum UT316A‐2 (UT316) and grown in sand (pH 4.8). Mycorrhizal (+VAM) plants had higher shoot and root dry matter (DM) than nonmycorrhizal (‐VAM) plants. The VAM treatment had significant effects on shoot concentrations of P, K, Ca, Fe, Mn, and Zn; shoot contents of P, S, K, Ca, Mg, Fe, Mn, Zn, and Cu; root concentrations of P, S, K, Ca, Mn, Zn, and Cu; and root contents of Al, P, S, K, Ca, Mg, Fe, Mn, Zn, and Cu. The VAM effects on nutrient concentrations and contents and DM generally followed the sequence of UT316 > UT143 > ‐VAM. The VAM isolate UT143 particularly enhanced Zn uptake, and both VAM isolates enhanced uptake of P and Cu in shoots and roots, and various other nutrients in shoots or roots.     

Changes in mineral nutrient concentrations and C‐N metabolism in cabbage shoots and roots following macronutrient deficiency

The responses of metabolic networks to mineral deficiency are poorly understood. Here, we conducted a detailed, broad‐scale analysis of macronutrient concentrations and metabolic changes in the shoots and roots of cabbage (Brassica rapa L. ssp. pekinensis) plants in response to N, P, K, Ca, and Mg deficiency in nutrient solution. To standardize individual macronutrient‐deficient treatments, the concentrations of the other nutrients were maintained via substitution with other ions. Individual nutrient deficiencies had various effects on the uptake and accumulation of other mineral nutrients. Phosphorus deficiency had relatively little effect on other mineral nutrient levels compared to the other treatments. Cation deficiency had little effect on N and P concentrations but had a somewhat negative effect on the uptake or concentrations of the other nutrients. Primary metabolic pathways, such as energy production and amino acid metabolism, were greatly affected by mineral nutrient deficiency. Compared to the control treatment, soluble sugar levels increased under –N conditions and decreased under –Ca and –Mg conditions. The levels of several organic acids involved in glycolysis and the TCA cycle decreased in response to –N, –P, or –K treatment. The levels of most amino acids decreased under ‐ N treatment but increased under –P, –K, –Ca, or –Mg treatment. Mineral depletion also led to the activation of alternative biochemical pathways resulting in the production of secondary metabolites such as quinate. Notable changes in metabolic pathways under macronutrient deficiency included (1) a quantitative increase in amino acid levels in response to Mg deficiency, likely because the restriction of various pathways led to an increase in protein production and (2) a marked increase in the levels of quinate, a precursor of the shikimate pathway, following cation (K, Ca, and Mg) deficiency. These findings provide new insights into metabolic changes in cabbage in response to mineral deficiency and pave the way for studying the effects of the simultaneous deficiency of more than one macronutrient on this crop.     

A Power Function Based Approach for the Assessment of the Sulfate Deprivation Impact on Nutrient Allocation in Young Maize Plants

Thirteen-day-old maize (Zea mays L.) plants were exposed hydroponically to sulfur (S)-deprivation and their nutritional status was monitored for ten days. Sulfur (S) -deprivation altered the allocation of nutrients between roots and shoots in a differential manner and the effect was approached in terms of a power function. The experimental curvature was analyzed through the value of the exponent of the function and two formulations of the approach were tested through regression analysis. In the shoot, the impact of the S-deprivation relative to that on dry mass was: calcium (Ca) (45.3%) > nitrate (NO₃) (18.9%) > magnesium (Mg) (17.2%) > manganese (Mn) (14.1%) > water (W) = phosphorus (P) = potassium (K) = iron (Fe) = copper (Cu) > nitrogen (N) (?4.3%) > ammonium (NH₄₎ (?4.7%) > zinc (Zn) (?12%) > boron (B) (?21.4%) > S (?75.2%). In the root, the relative impact was: N = K = Ca = Zn = B > P (?12.7%) > NO₃ (?14.7%) > NH₄ (?18.4%) > W (?27.8%) > Mn (?34.4%) > Mg (?37.5%) > Fe = Cu (?40.5%) > S (?126.6%). Both models produced the same conclusions.     

The effect of mycorrhizal inoculation and phosphorus application on the growth and mineral nutrient status of apple seedlings

Apple seedlings cv. Antonovka were grown in soil taken from an orchard with a distinctive specific apple replant disease. The influence of the different available soil phosphorus (P) level (0, 20, 40 and 80 mg P dm^?3 of soil) and arbuscular mycorrhizae fungi (AMF) inoculation on the vegetative growth, chlorophyll fluorescence, and the frequency of mycorrhizae were assessed. Moreover, leaf and root mineral composition was ascertained by means of the inductively coupled plasma mass spectrometry (ICP-MS) method. The inoculation with AMF influenced seedlings growth as well as the biomass production and partitioning. The method of inoculation (granular, quick root dip or irrigation) had a great impact on the frequency of mycorrhizae (83.3, 98.8 and 100%, respectively) as well as on the abundance of arbuscules (36.4, 62.9 and 67.3%) as compared to the control (11.7%). The beneficial effect of AMF on leaf PSII efficiency was established. AMF inoculated plants had a significantly higher content of nitrogen, potassium, phosphorus and boron (N, K, P and B) in the shoots and a higher content of nitrogen, sulfur, copper, iron, manganese, molybdenum and titanium (N, S, Cu, Fe, Mn, Mo and Ti) in the roots. Although roots showed a higher concentration of aluminium, barium, lithium, cadmium, lead and vanadium (Al, Ba, Li, Cd, Pb, and V) but upon AMF inoculation, the concentration of these cations was much lower.     

Changes in C and N fractions with composted manure plus chemical fertilizers applied in apple orchard soil: an in‐situ field incubation study on the Loess Plateau,China

We investigated the effects of compost (CM ), made from poultry and cattle manure with spent mushroom substrate, plus chemical fertilizers (CF s) on soil organic carbon (C) and nitrogen (N) fractions in silty loam soil of the Loess Plateau. Eight fertilizer practices were applied in a 7‐year‐old ‘Red Fuji’ apple (Malus domestica Borkh.) orchard for 360 days. Compared to CM alone, CM –CF s decreased slightly soil total organic C but increased total N by 4.3–11.6%. Notably, CM –CF s increased soil microbial biomass C (MBC ) by 2.7–26.5% and microbial biomass N (MBN ) by 7–13.7%. Soil water‐soluble carbon (WSC ) was increased by 20.7% and 19.2% when 2% CM plus N and phosphorus (P) (2%M–NP ) and 4% CM plus N and P (4%M–NP ) were applied, respectively. Whereas 0.5% CM plus N and P (0.5%M–NP ) increased WSC by 9.3% on day 30 but decreased it by 7.2% from 30–360 days. Hot water‐soluble C increased by 13.1–14.6% from 0–180 day, but thereafter, the effect disappeared. Compared to CF s, CM –CF s increased MBN by 35.1–115.6%, and increased alkali‐hydrolyzable‐N by 3.5–55.8% over 180–360 days of incubation. Additionally, CM –CF s promoted N mineralization, increasing NH ₄‐N and NO ₃‐N contents. Based on the changes in C and N fractions and available nutrients, 2%M–NP (45 t/ha of CM plus 450 kg/ha of N and 157.5 kg/ha of P) may be the optimal fertilizer strategy for stimulating soil microbial growth and activity, and enhancing nutrient cycling for apple growth.     

Diagnosis and Recommendation Integrated System for Monitoring Nutrient Status of Mango Trees in Submountainous Area of Punjab,India

Abstract

The Diagnosis and Recommendation Integrated System (DRIS) was used to identify nutrient status of mango fruit trees in Punjab, India. Standard norms established from the nutrient survey of mango fruit trees were 1.144, 0.126, 0.327, 2.587, 0.263, 0.141% for nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S), and 15, 3.5, 145, 155, and 30 mg kg^?1, respectively, for zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), and boron (B) in dry matter. On the basis of DRIS indices, 16, 15, 12, 17, and 16% of total samples collected during nutrients survey of mango trees were low in N, P, K, Ca, and Mg, respectively. For micronutrients, 19, 18, 12, 20, and 6% samples were inadequate in Zn, Cu, Fe, Mn, and B, respectively. DRIS‐derived sufficiency ranges from nutrient indexing survey were 0.92–1.37, 0.08–0.16, 0.21–0.44, 1.71–3.47, 0.15–0.37, and 0.09–0.19% for N, P, K, Ca, Mg, and S and 11–19, 1–6, 63–227, 87–223, and 16–44 mg kg^?1 for Zn, Cu, Fe, Mn, and B, respectively.     

The effects of arsenic on the growth and nutritional status of Anadenanthera peregrina,a Brazilian savanna tree

Anadenanthera peregrina is a Brazilian savanna tree species that occurs naturally in arsenic (As)‐contaminated areas, and its As resistance has been associated with arbuscular mycorrhizal–fungi (AMF) symbiosis. A plant's ability to survive in stressful environments is correlated with its nutrition status, which can be affected by As uptake. The present study evaluated the influence of As on the concentrations and distribution of nutrients in the roots and shoots of A. peregrina grown in the absence of AMF. These plants were grown in substrates spiked with 0, 10, 50, and 100 mg As kg^–1 for 25 d under greenhouse conditions, and the concentrations of essential macro‐ (P, K, Ca, Mg, N, and S) and micro‐ (Fe, Mn, Cu, Zn, B, and Mo) nutrients in the roots and shoots were then determined. Enhanced As levels increased the concentrations of P, S, and N and decreased Ca, Mg, and Fe. Although the deleterious effects of As on the plants were striking, the internal As levels were high, which indicated some tissue tolerance of A. peregrina.     

硼对烤烟干物质积累和养分吸收的动态模拟

采用溶液培养方法,研究了不同硼浓度下烤烟干物质积累动态和养分吸收动态变化特点。结果表明,不同硼浓度下烤烟干物质积累动态和养分吸收动态可用Logistic方程描述。烤烟地上部分和根部干物质积累最大速率和养分吸收最大速率出现日期不同。BO33-浓度为0～200.mg/L的各处理烤烟植株地上部氮、磷、钾养分吸收最大速率出现在干物质积累最大速率之前;而根部则出现在干物质积累最大速率之后。BO33-浓度为0.25～2.00.mg/L的各处理烤烟干物质积累和养分吸收最大速率明显高于其他3个处理,并且最大积累速率出现时间均相对较晚。     

菌根化育苗对玉米生长和养分吸收的影响

【目的】丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)侵染作物根系形成菌根共生体系对于作物吸收磷具有重要作用,但该结果大多来源于室内受控试验,有限的田间试验因环境条件、试验材料与接种技术等差异致使AMF菌剂应用效果不一。本研究通过玉米菌根化育苗和田间移栽,分析了接种AMF对玉米生长、养分吸收、籽粒产量及养分含量的影响,以期推进菌根技术的实际生产应用。【方法】以自交品系玉米B73为供试作物,于2018年5月至10月在北京市延庆区进行了田间试验。田间小区设置基施磷(+P)和不施磷(–P)处理。供试AMF为Rhizophagus irregularis Schenck&Smith BGC AH01。玉米种子催芽后,分别播入加入AMF菌剂(+M)和菌剂过滤液(–M)的育苗钵内,培养两周后移栽至田间。玉米在田间条件下生长至拔节期时,使用便携式光合仪测定叶片光合速率与气孔导度,取样测定地上部与根部干重和养分元素含量,同时测定菌根侵染率;在玉米完熟期取样,测定籽粒百粒重、籽粒产量及养分含量。【结果】无论田间施磷与否,接菌植株根系的菌根侵染强度和丛枝丰度均显著高于不接菌植株。不施磷情况下,+M处理显著提高了玉米根系干重,玉米生长的菌根依赖性(163.7%)显著高于施磷情形(124.1%)。–P–M处理玉米叶片的光合速率和气孔导度显著低于其他3个处理。–P+M处理玉米叶片的光合参数、玉米地上部和根部磷含量与+P+M均无显著差异。与–P–M处理相比,–P+M显著提高了玉米籽粒产量和百粒重,同时也提高了籽粒中锌、锰、镁等矿质养分的含量,且与+P+M处理相比均无显著差异。【结论】玉米幼苗接种AMF后再移栽到田间,可以显著提高拔节期玉米根系的菌根侵染率,促进玉米地上部和根部对磷及锌、锰和镁的吸收,进而促进玉米的生长,提高籽粒产量和养分含量。本试验条件下,菌根化育苗可以达到与施磷同样的效果,在保障作物不减产的前提下减少磷肥施用量。     

钾、镁交互作用对橡胶幼苗生长及养分吸收的影响

  【目的】  我国植胶区砖红壤钾、镁缺乏现象日益突出,研究钾、镁缺乏对橡胶幼苗根系形态和养分吸收的影响,可为橡胶平衡施肥和优质高产栽培提供理论依据。  【方法】  选用‘热研7-33-97’橡胶 (Hevea brasiliensis) 幼苗为研究材料,在人工气候箱内用营养液培养。采用二因素二水平的析因试验设计,设置4个处理:对照 (CK)、缺钾 (–K)、缺镁 (–Mg) 和缺钾镁 (–K-Mg),培养3个月后,取样测定橡胶幼苗干物质量、根系构型参数、根系活力和养分含量等指标。  【结果】  1) 与CK相比,–K和–K-Mg处理显著降低了单株干物质量和根冠比,干物质量降幅分别为8.4%和27.5%,根冠比降幅分别为20.4%和26.9%,而–Mg处理对干物质量和根冠比均无显著影响;K、Mg交互作用对茎干、根和单株干物质量及根冠比均有显著影响 (P < 0.05)。2) 与CK相比,各缺素处理均显著降低了橡胶幼苗吸收根 (直径 < 2 mm) 的根长、根表面积、根体积、总根尖数及根系活力等根系构型参数,而不同程度增加了平均根粗。方差分析结果表明,K、Mg交互作用对吸收根的根长、根表面积、根体积及总根尖数有极显著影响 (P < 0.01)。3) 各处理下氮和镁、磷和钾以及钙分别在叶片、根系以及茎皮中的平均分配比例高于其他器官。各缺素处理下,地上部的养分占比呈增加趋势。4) 与CK相比,–K处理显著增加了橡胶幼苗单株氮、磷和镁的积累,–K-Mg处理则显著降低了单株氮积累,各缺素处理均显著增加了单株钙的积累;K、Mg交互作用对氮、磷、钙和镁的积累有显著或极显著影响。  【结论】  钾、镁营养显著影响橡胶幼苗对养分的吸收,缺钾、缺镁显著抑制橡胶幼苗特别是根系的生长发育,同时缺钾缺镁加重抑制效果。因此,橡胶生产上不仅要保证培养基质或土壤的矿质营养充足,还要重视钾、镁元素间平衡关系。     

Macronutrient Balance of Nickel-Stressed Lettuce Plants Grown under Different Sulfur Levels

This study investigated whether intensive nutrition with sulfur–sulfate (S-SO₄) (2, 6, or 9 mM S) of nickel-stressed (0, 0.0004, 0.04, or 0.08 mM Ni) butterhead lettuce cv. Justyna may improve macronutrient balance and reduce Ni bioaccumulation. Nickel exposure resulted in various unfavorable changes in the macronutrient content together with increase of Ni accumulation in the biomass. Intensive S nutrition of Ni-treated lettuce seems to have no beneficial effect on macronutrient balance. In general, it significantly reduced the root and foliar phosphorus (P), potassium (K), calcium (Ca), and S content and simultaneously did not affect the magnesium (Mg) content in the biomass. At the same time, the nitrogen (N) content was reduced in roots and elevated in shoots. Supplementation of Ni-exposed lettuce with high S doses raised in roots and reduced foliar Ni accumulation; however, Ni content in useable parts exceeded the acceptable limits established for consumption.     

Responses of barley to hypoxia and salinity during seed germination,nutrient uptake,and early plant growth in solution culture

The resistance of most plants to salt can be impaired by concurrent waterlogging. However, few studies have examined this interaction during germination and early seedling growth and its implications for nutrient uptake. The aim of the study was to examine the response of germination, early growth, and nutrient uptake to salt (NaCl) and hypoxia applied to barley (Hordeum vulgare L. cv. Stirling), in solution culture. Hypoxia, induced by covering seeds with water, lowered the germination from 94% to 28% but salinity and hypoxia together lowered it further to 13% at 120 mM NaCl. While the germination was 75% at 250 mM NaCl in aerated solution, it was completely inhibited at this NaCl concentration under hypoxia. Sodium ion (Na⁺) concentrations in germinated seedlings increased with increasing salinity under both aerated and hypoxic conditions during germination, while K⁺ and Mg⁺ concentrations were decreased with increasing salinity in 6 d old seedlings. After 20 d, control seedlings had the same dry weights of the roots and shoots with and without hypoxia but at 10 mM NaCl and higher, shoot and root dry weight was depressed with hypoxia. Sodium ion increased in roots and shoots with increased NaCl under both aerated and hypoxic conditions while K⁺ was depressed when salinity and hypoxia were applied together and Ca²⁺ was mostly decreased by NaCl. In general, hypoxia had greater effects on nutrient concentrations than NaCl by decreasing N, P, S, Mg, Mn, Zn, and Fe in shoots and by increasing B concentrations. The threshold salinity levels decreased markedly for germination, uptake of a range of nutrients, and for seedling growth of barley under hypoxic compared to well‐aerated conditions.     

Identifying nutrient imbalances in pomegranate (Cv. Bhagwa) at different phonological stages by the diagnosis and recommendation integrated system

The diagnosis and recommendation integrated system (DRIS) approach was used to interpret nutrient analyses of leaf tissues from pomegranate cv. Bhagwa orchards grown in southwestern Maharashtra, India. The DRIS norms were established for three growth stages,viz. 50% flowering, fruit development and first harvesting of pomegranate. Various nutrient ratios were obtained from high-yielding population and were used to compute DRIS indices for diagnosing nutrient imbalances and their order of limitation to yield. Nutrient sufficiency ranges at 50% flowering derived from DRIS norms were 1.32–2.15% nitrogen (N), 0.18–0.24% phosphorus (P), 1.29–1.99% potassium (K), 0.64–1.20% calcium (Ca), 0.23–0.45% magnesium (Mg), 0.16–0.26% sulfur (S), 103.04–149.12 mg kg^?1 iron (Fe), 39.60–72.85 mg kg^?1 manganese (Mn), 15.99–26.10 mg kg^?1 zinc (Zn), 6.16–9.32 mg kg^?1 copper (Cu), 23.38–39.88 mg kg^?1 boron (B) and 0.29–0.47 mg kg^?1 molybdenum (Mo). Similarly, the sufficiency range at fruit development and first harvesting was developed for computing DRIS indices. The requirement of Fe, Mg, S, Zn and N by the pomegranate plant was higher at 50% flowering and fruit development stages. According to these DRIS-derived indices, 87.85, 73.83, 70.09, 69.16 and 65.42% orchards were deficient in Fe, S, Mg, Zn, and N, respectively, at 50% flowering, while 70.03, 66.36, 63.55, 61.68, and 68.22% orchards were found to be deficient in respective nutrients during the fruit development stage.     

Nutrient and carbon balances in organic vegetable production on an irrigated,sandy soil in northern Oman

Our understanding of nutrient and carbon (C) fluxes in irrigated organic cropping systems of subtropical regions is limited. Therefore, leaching of mineral nitrogen (N) and phosphorus (P), gaseous emissions of NH₃, N₂O, CO₂, and CH₄, and total matter balances were measured over 24 months comprising a total cropping period of 260 d in an organic‐cropping‐systems experiment near Sohar (Oman). The experiment on an irrigated sandy soil with four replications comprised two manure types (ORG1 and ORG2) characterized by respective C : N ratios of 19 and 25 and neutral detergent fiber (NDF)‐to‐soluble carbohydrates (SC) ratios of 17 and 108. A mineral‐fertilizer (MIN) treatment with equivalent levels of mineral N, P, and potassium (K) served as a control. The three treatments were factorially combined with a cropping sequence comprising radish (Raphanus sativus L.) followed by cauliflower (Brassica oleracea L. var. botrytis) or carrot (Daucus carota subsp. sativus). Over the 24‐months experimental period gaseous N emissions averaged 45 kg ha^–1 (59% NH₃‐N, 41%N₂O‐N) for MIN, 55 kg N ha^–1 (69% NH₃‐N, 31%N₂O‐N) for ORG1, and 49 kg N ha^–1 (59% NH₃‐N, 41% N₂O‐N) for ORG2. Carbon losses were 6.2 t ha^–1 (98% CO₂‐C, 2% CH₄‐C) for MIN, 9.7 t C ha^–1 (99% CO₂‐C, 1% CH₄‐C) for ORG1, and 10.6 t ha^–1 (98% CO₂‐C, 2% CH₄‐C) for ORG2. Exchange resin–based cumulative leaching of mineral N amounted to 30 kg ha^–1 for MIN, 10 kg ha^–1 for ORG1, and 56 kg ha^–1 for ORG2. Apparent surpluses of 361 kg N ha^–1 and 196 kg P ha^–1 for radish‐carrot and 299 kg N ha^–1 and 184 kg P ha^–1 for radish‐cauliflower were accompanied by K deficits of –59 kg ha^–1 and –73 kg ha^–1, respectively, for both cropping systems. Net C balances for MIN, ORG1, and ORG2 plots were –7.3, –3.1, and 1.5 t C ha^–1 for radish‐carrot and –5.0, 1.3, and 4.6 t C ha^–1 for radish‐cauliflower. The results underline the difficulty to maintain soil C levels in intensively cultivated, irrigated subtropical soils.     

Effect of supplied phytosiderophore on 59Fe absorption and translocation in Fe-deficient barley grown hydroponically in low phosphorus media

Abstract

Hydroponically grown barley plants (Hordeum vulgare L. cv. Minorimugi) under iron-deficient (–Fe) and high phosphorus (P) conditions (500 µmol L^?1) showed Fe chlorosis and lower growth compared with plants grown in –Fe and low P conditions (50, 5 and 0.5 µmol L^?1). To understand the physiological role of P in regulating the growth of plants in –Fe medium, we carried out an Fe feeding experiment using four P levels (500, 50, 5 and 0.5 µmol L^?1) and phytosiderophores (PS), mugineic acid. Our results suggest that plants grown in a high P medium had higher absorption activity of ⁵⁹Fe compared with plants grown in low P media, irrespective of the presence or absence of added PS. Translocation of ⁵⁹Fe from roots to shoots was not affected by the P level. The relative translocation rate of ⁵⁹Fe increased with decreasing levels of P in the medium. In general, the addition of PS enhanced the absorption of ⁵⁹Fe and its translocation. Taken together these results suggest that the lower relative translocation rate of Fe in high P plants may be induced by the physiological inactivation of Fe in the roots, and the higher absorption activity of Fe in high P conditions possibly results from the response of barley plants to Fe deficiency.     

Rhizosphere dynamics under nitrogen‐induced root modification: The interaction of phosphorus and calcium

Optimizing root phosphorus (P) acquisition to reduce intensive fertilizer use is a crucial pathway for sustainable agriculture, particularly as P is an important plant macronutrient, often limiting in a majority of soils worldwide. Although many studies have assessed plant growth and P acquisition, few studies have investigated the interactive effects of nitrogen (N)‐induced root modification on soil P processes or the understudied effects of soil calcium (Ca) dynamics on soil P bioavailability. In this study, we investigate soil P and Ca response in the rhizosphere of durum wheat (Triticum turgidum L. spp. durum). Wheat grown under controlled conditions preloaded for 20 d with two N treatments [preloaded low N (1 mmol KNO₃ plant^?1) and preloaded high N (2 mmol KNO₃ plant^?1)] were transferred to rhizoboxes for 12 d [days after transfer (DAT)]. Shoot and root biomass, P and Ca concentration, and plant‐available P and extractable Ca were determined every three days (0, 3, 6, 9, 12 DAT). Significantly higher root mass (P = 0.7%), root length (P = 1.8%) and total biomass (P = 2.2%) were found at the end of the experiment but exclusively for high N preloaded wheat. This greater root biomass was associated with lower root P concentration, suggesting a dilution response, while little difference was observed in shoot P concentration over the 12 d. However, Ca accumulated in both roots and shoots under both preloading N levels. Concurrently, soil‐extractable Ca declined, and plant‐available P increased (r = –0.62; P = 0.03%), presumably due to a promoting effect of Ca uptake on soil P availability; lower soil Ca in turn increased the repulsive forces between P ions and the negatively charged soil surface, resulting in an increased P availability in the soil solution. This study contributes to the understanding of the complex interplay between multi‐nutrient dynamics within the rhizosphere.     

缺磷对不同作物根系形态及体内养分含量浓度的影响

采用营养液培养方法,以水稻、 小麦、 玉米和大豆为试验材料,研究了短期缺磷（2周）诱导根表沉积铁氧化物是否为水稻特有的性质,以及缺磷对不同作物根系形态及其吸收钾、 钙、 铁、 锰、 铜、 锌营养元素的影响。结果表明,供磷和缺磷处理并没有影响小麦、 玉米和大豆3种作物根系的颜色,而缺磷处理水稻根表沉积了铁氧化物而呈红（黄）棕色,且铁氧化物不均匀地富集在根细胞壁的孔隙中; 缺磷促进了水稻,小麦,玉米和大豆根系的生长,分别比供磷处理伸长了11%、 11%、 20%和11%（P0.05）。此外,缺磷胁迫下水稻根表铁氧化物增强了钙、 铁、 锰、 铜和锌在根表的富集而成为其进入根系的缓冲层。缺磷处理水稻根中铁浓度明显高于供磷处理（P0.05）,而地上部铁的浓度仅为磷营养正常水稻植株的18%,这说明缺磷诱导的铁氧化物促进了根系对铁的吸收但抑制了铁由根系向地上部的转运。短期缺磷对其他养分在水稻根中和地上部的浓度没有明显影响。对于其他 3 种作物,短期缺磷没有明显影响钾、 钙、 铁、 锰、 铜和锌在其根表富集及在植物体内的浓度。因此,在供试的4 种作物中,由于磷胁迫诱导根表形成铁氧化物是水稻特有的性质,铁氧化物的沉积可促进铁的吸收但抑制了铁向地上部的转运,而短期缺磷并没有影响其他3种作物对钾、 钙、 铁、 锰、 铜和锌养分的吸收和转运。     

Alleviation of Multinutrient Deficiency for Productivity Enhancement of Rain-Fed Soybean and Finger Millet in the Semi-arid Region of India

Soil nutrient contents were determined in 802 surface soil samples (0–15 cm deep) collected from farmers' fields that support extensive cultivation of soybean (Glycine max L.) and finger millet (Eleusine coracana G.), spread across three districts, in the semi-arid regions of Karnataka, India. Following soil analysis, on-farm crop trials were conducted during 2005–2007 to study the crop response to the soil application of nitrogen (N), phosphorus (P), sulfur (S), boron (B), and zinc (Zn) fertilizers. Analyses of soil samples revealed that 4–83% fields were deficient in N, 34–65% in P, 83–93% in extractable S, 53–96% in B, and 34–88% of farmers' fields were deficient in Zn. On-farm trials conducted during the three rainy seasons (2005, 2006, and 2007) significantly (P ≤ 0.05) enhanced crop productivity indices such as yields of grain, stover, and total biomass in soybean and finger millet crops. Integrated management of deficient nutrients in finger millet and soybean crops significantly enhanced the grain and straw uptake of N, P, K, S, and Zn.     

Release of carbon and nitrogen from fodder radish (Raphanus sativus) shoots and roots incubated in soils with different management history

Plant roots are generally considered to decompose slower than shoots and contribute more to accumulation of soil organic matter, and management history is expected to shape the structure and function of decomposer communities in soil. Here we study the effect of chemical characteristics of shoots and roots from fodder radish (Raphanus sativus oleiformis L.), a widely used cover crop, on the release of their C and N after addition to soil. Shoots and roots were incubated for 180?d at 20°C using four soils with different management histories (organic versus mineral fertiliser, with and without use of cover crops), and the release of CO₂ and extractable mineral N was determined. More shoot C than root C was mineralised during the first 10?d of incubation. After 180?d, 58% of the C input was mineralised with no difference between shoots and roots. At the end of incubation, shoots had released more N (42% of shoot N) than roots (28% of root N). Moreover, management history did not affect net mineralisation of added plant C. Residues incubated in soil with a management history involving cover crops showed an enhanced net N mineralisation. Therefore, long-term decomposition of C added in radish shoots and roots is unaffected by differences in chemical characteristics or soil management history. However, the net mineralisation of N in shoots is faster than for N in roots, and net N mineralisation of added materials is higher in soil with than without a history of cover crops.

Abbreviations: CC: cover crop; IF: inorganic fertilizer; M: manure     

不同基质对平邑甜茶幼树生长、根系形态与营养吸收的影响

采用盆栽方式,探讨了栽培于腐熟羊粪、沙土、轻粘土中的平邑甜茶[Malus hupehensis(Pamp) Rehd] 幼树的生长、根系形态与营养吸收的特性。结果显示,羊粪处理平邑甜茶幼树新梢生长量最大,主根和侧根粗长、侧根多,对磷、钙及铁的吸收能力较低;沙土处理的新梢生长量最小,侧根细及少,对磷、钾、钙、铁及锌等元素的吸收能力强;粘土处理的新梢生长量和根系特征参数居于羊粪和沙土处理之间。沙土施羊粪后,平邑甜茶幼树新梢生长量增大,主根增粗、增长,侧根增多且粗长,对磷、钾、钙、铁及锌等营养元素的吸收能力明显下降;而粘土施羊粪,植株叶片增多,主根粗度及长度降低,二级侧根增多、增粗,对钾的吸收能力提高。