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1.
一株PVYNTN-NW黑龙江马铃薯分离物的检测鉴定 总被引:1,自引:0,他引:1
马铃薯Y病毒(Potato virus Y,PVY)是马铃薯、烟草等茄科作物上的重要病毒,在与寄主共同进化过程中产生了许多株系。本文从黑龙江马铃薯样品中得到PVY分离物A12。ELISA结果表明A12被PVYO的单克隆抗体特异性识别。A12开放阅读框为9 186个核苷酸,编码3 061个氨基酸,与SYR-II-Be1分离物的核苷酸和氨基酸序列一致率均最高,分别为98.3%和99.2%。系统发育分析发现A12与PVYNTN-NW株系SYR-II型的分离物聚类到一起;重组分析表明,A12是N-605和Oz的重组体,重组类型与SYR-II-Be1相同。综合以上结果表明,A12属于PVYNTN-NW株系SYR-II型。但与常见PVYNTN-NW株系分离物在珊西烟引起叶脉坏死不同,A12产生花叶症状。A12辅助成分-蛋白酶在182位和245位的氨基酸均为精氨酸,而其它PVYNTN-NW株系分离物为赖氨酸。本研究结果可为黑龙江马铃薯PVY的早期检测和有效防控提供理论指导。 相似文献
2.
马铃薯Y病毒(Potato virus Y,PVY)是马铃薯、烟草等茄科作物上的重要病毒,在与寄主共同进化过程中产生了许多株系。本文从黑龙江马铃薯样品中得到PVY分离物A12。ELISA结果表明A12被PVYO的单克隆抗体特异性识别。A12开放阅读框为9 186个核苷酸,编码3 061个氨基酸,与SYR-II-Be1分离物的核苷酸和氨基酸序列一致率均最高,分别为98.3%和99.2%。系统发育分析发现A12与PVYNTN-NW株系SYR-II型的分离物聚类到一起;重组分析表明,A12是N-605和Oz的重组体,重组类型与SYR-II-Be1相同。综合以上结果表明,A12属于PVYNTN-NW株系SYR-II型。但与常见PVYNTN-NW株系分离物在珊西烟引起叶脉坏死不同,A12产生花叶症状。A12辅助成分-蛋白酶在182位和245位的氨基酸均为精氨酸,而其它PVYNTN-NW株系分离物为赖氨酸。本研究结果可为黑龙江马铃薯PVY的早期检测和有效防控提供理论指导。 相似文献
3.
马铃薯Y病毒(potato virus Y,PVY)主要侵染马铃薯和烟草等茄科作物,给世界农业造成巨大经济损失。本文对测定的23个及GenBank中注册的52个中国PVY分离物ORF序列进行了系统发育、重组和选择压等分析。系统发育分析表明,根据ORF序列可把我国75个PVY分离物和国外30个参比分离物分成O、C、E、NTN-NW(SYR-I型)、NTN-NW(SYR-II型)、NTN(NTN-a型)、NTN(NTN-b型)、NA-N/NTN、Eu-N、N-Wi(N:O型)和N-Wi(N-Wi型)等11个分子株系,其中中国PVY分离物属于除E和C株系外的9个分子株系。除ME162、guiyang、PVYzu、SD-G、WA-13和CN:JL-1:17等 6个分离物基因组中未检测到重组,其余69个分离物均存在明显重组。根据重组位点的不同,中国PVY可分为11种重组类型,其中5种为新的重组类型。选择压分析表明,中国PVY分离物的11个基因均处于负选择,其中核内含体b基因受到的选择压最大,PIPO受到的选择压最小。基因流分析表明,黑龙江、河南和山东PVY分离物间基因交流频繁,马铃薯与烟草PVY分离物之间基因交流频繁。本研究的结果明确了中国PVY分离物的分子株系组成,对指导PVY的检测和防控具有积极作用。 相似文献
4.
马铃薯是我国重要粮食和经济作物。马铃薯Y病毒(potato virus Y,PVY)是危害马铃薯生产的重要病害。种植脱毒种薯是防治PVY最有效的途径。马铃薯种薯携带PVY问题严重,但种薯中PVY株系还不清楚。本研究利用PVY特异性抗体检测了7个马铃薯品种362个种薯,发现不同品种种薯带毒率差异较大,最高达12%。通过RT-PCR方法扩增获得了7个PVY分离物编码区全序列。重组分析发现7个分离物基因组均为重组型,根据重组位点的差异可以分为PVYNTN-NW(SYR-II型)、Rec-1、Rec-2和Rec-3等4种重组类型,后3种为新重组类型。系统进化分析发现,分离物HQH18G3-10与PVYNTN-NW(SYR-II型)处于同一个大的分支,但与中国PVY大田分离物聚集在一起形成一个相对独立的组,命名为PVYNTN-NW(CN型);其余6个分离物与数据库中的中国分离物聚集在PVYN-Wi组。这暗示PVY中国分离物具有相对独立的进化过程,PVY马铃薯大田分离物和种薯分离物进化上相近。所有分离物均能在珊... 相似文献
5.
马铃薯Y病毒 (potato virus Y,PVY) 是一种重要的农作物病毒,可造成产量损失和产品质量下降。其宿主范围广泛,包括马铃薯、烟草、番茄和辣椒等经济作物。在广西从叶片表现斑驳褪绿症状的马铃薯上分离到一株PVY分离物DX,其基因组包含一个大的开放阅读框 (open reading frame,ORF),由9 186 nt组成,编码3 061个氨基酸。系统发育进化分析显示,分离物DX与PVYN-Wi株系分离物IUNG-12、SGS-AG、MAF-VOY聚类成一个分支。重组分析表明分离物DX基因组在496 nt和2 388 nt存在重组位点,分别位于P1和HC-Pro/P3结合区,是分离物Oz和N605的重组体。通过机械摩擦接种,分离物DX可侵染茄科9种作物,引起本生烟叶片花叶、皱缩和泡状突起等症状;引起普通烟草和番茄的轻微花叶症状;引起马铃薯叶片花叶症状,接种辣椒没有发病。序列比对分析显示,分离物DX缺乏引起烟草叶脉坏死相关的氨基酸位点N205、K400和E419。本研究比较了分离物DX对茄科共12种作物的侵染能力和病害症状差异,结果表明分离物DX可用于探索PVY在不同寄主中的致病机理。 相似文献
6.
本研究以马铃薯Y病毒(PVY)全基因组为基础,分析吉林、黑龙江和内蒙古3省(区)PVY群体遗传多样性和群体分化,并评估突变、重组、选择等遗传力所起的作用。根据已报道的PVY全基因序列保守区设计4对引物,采用片段重叠法对来自内蒙古和吉林的24个PVY分离物全基因序列进行测定,并联合NCBI中已登录的9个黑龙江分离物全基因组序列进行遗传多样性参数评估、群体分化检验和分子变异等分析。结果显示,我国北方3省(区)PVY群体遗传多样性高,其中内蒙古和黑龙江PVY群体遗传多样性高于吉林群体,并且3个群体之间呈现一定程度的遗传分化。分子变异分析发现在PVY基因组中存在1 786个变异位点,表明我国北方3省(区)PVY群体变异程度较高,并且这种高变异度有85.54%来自各个马铃薯种植区内PVY个体的遗传变异。重组分析和系统发育分析发现,我国北方3省(区)PVY群体中重组株系占比高达90.3%,并具有明显的株系多样性,表明PVY重组株系已成为我国北方3省(区)马铃薯种植区的流行株系。选择压力分析显示,使用FEL和IFEL法分别检测出501个和315个净化压力选择位点,这表明3省(区)PVY群体受净化选择压力为主。以上结果表明,中国北方3省(区)PVY群体遗传多样性高,突变、重组和自然选择都对遗传多样性和群体分化存在一定影响。 相似文献
7.
马铃薯是我国重要粮食和经济作物。马铃薯Y病毒(potato virus Y,PVY)是危害马铃薯生产的重要病害。种植脱毒种薯是防治PVY最有效的途径。马铃薯种薯携带PVY问题严重,但种薯中PVY株系还不清楚。本研究利用PVY特异性抗体检测了7个马铃薯品种362个种薯,发现不同品种种薯带毒率差异较大,最高达12%。通过RT-PCR方法扩增获得了7个PVY分离物编码区全序列。重组分析发现7个分离物基因组均为重组型,根据重组位点的差异可以分为PVYNTN-NW(SYR-II型)、Rec-1、Rec-2和Rec-3等4种重组类型,后3种为新重组类型。系统进化分析发现,分离物HQH18G3-10与PVYNTN-NW(SYR-II型)处于同一个大的分支,但与中国PVY大田分离物聚集在一起形成一个相对独立的组,命名为PVYNTN-NW(CN型);其余6个分离物与数据库中的中国分离物聚集在PVYN-Wi组。这暗示PVY中国分离物具有相对独立的进化过程,PVY马铃薯大田分离物和种薯分离物进化上相近。所有分离物均能在珊西烟上引起典型叶脉坏死症状,HQH18G3-10引起的坏死症状最为严重。本研究首次报道了我国种薯内PVY发生情况,对分析病毒发生发展规律和防控具有借鉴作用。 相似文献
8.
马铃薯是重要的粮食和经济作物。马铃薯Y病毒(potato virus Y,PVY)是危害马铃薯安全生产的重要病毒。近年来,危害我国马铃薯的PVY株系组成发生了显著变化。PVY重组型株系尤其是PVYNTN-NW SYRI和SYRII型成为优势株系,但与传统株系分离物相比,优势株系分离物在不同寄主上的侵染性及其致病力还不清楚。本研究分析了PVYN株系代表性分离物PVYN605和PVYNTN-NWSYRI型分离物GZ在本氏烟、普通烟和辣椒上的侵染性,比较了二者在本氏烟和普通烟上的致病力。结果表明,PVYN605和PVYNTN-NWSYRI-GZ分离物均能侵染本氏烟和普通烟,并在普通烟上引起叶脉坏死;PVYN605不能系统侵染辣椒品种‘特大牛角王’,而PVYNTN-NWSYRI-GZ可系统侵染辣椒品种特大牛角王。PVYNTN-NWSYRI-GZ在本氏烟细胞间的移动速度明显慢于PVYN 相似文献
9.
一个马铃薯Y病毒山东分离物的分离与鉴定 总被引:4,自引:1,他引:4
从具有典型花叶症状的马铃薯叶片中分离到马铃薯Y病毒(Potato virus Y,PVY)(本文称PVY-SD-TA分离物),扩繁后,提纯病毒,电镜下可观察到700~900 nm×11 nm的病毒粒体,病组织超薄切片观察可见风轮状的内含体结构,寄主反应特性研究表明其能侵染2科13种植物。SDS-PAGE电泳检测病毒编码的外壳蛋白亚基的分子量为33 kDa。以PVY-SD-TA基因组RNA为模板,应用RT-PCR方法和特异引物合成了外壳蛋白基因。对cDNA全序列分析表明,PVY-SD-TA CP基因核苷酸序列与N株系的同源性为96%,与GenBank中登录序列号为AJ390306的O株系分离物的同源性最高,为99%;与国内不同学者报道的PVY中国流行株的同源性分别为96%,97%和98%。通过以上生物学特性和分子水平的研究将PVY-SD-TA鉴定为普通株系(PVYO株系)。 相似文献
10.
马铃薯Y病毒 (Potato virus Y,PVY) 是世界范围内对茄科属植物造成影响的重要病毒,为了解PVY对新疆加工番茄的危害,本文采集205份加工番茄样品进行血清学检测,结果PVYO、PVYO\N\C、PVYN检出率分别为20%、18.05%和0%,且PVY不同株系总检出率达24.9%。根据血清学检测结果,在苋色藜 (Chenopodium amaranticolor) 上进行单斑分离获得2个PVY分离物S1-10、S2-12,其基因组序列分析结果显示,S1-10分离物是PVYO与PVYN的重组体,属于PVYN∶O株系,S2-12属于PVYO株系。本研究表明新疆加工番茄上的PVY主要为PVYN∶O和PVYO株系。 相似文献
11.
J. F. J. M. van den Heuvel R. A. A. van der Vlugt M. Verbeek P. T. de Haan H. Huttinga 《European journal of plant pathology / European Foundation for Plant Pathology》1994,100(5):347-356
An Austrian isolate of potato virus YNTN, the causal agent of potato tuber necrotic ringspot disease (PTNRD), was serologically compared with seven Dutch PVYN isolates. Using polyclonal and monoclonal antibodies, it was found indistinguishable from PVYN. Determination of the nucleotide sequence of the coat protein cistron and comparison of the deduced amino acid sequence with coat protein sequences of other potyviruses revealed a high level of homology with PVYN coat protein sequences. This confirmed the close taxonomic relationship of PVYNTN with the PVYN subgroup of potato virus Y. PVYNTN is able to overcome all resistance genes known so far in commercial potato cultivars. Remarkably, transgenic PVY-protected tobacco plants are also resistant to PVYNTN infection upon mechanical and aphid-mediated inoculation. These experiments indicate that genetically engineered resistance offers great potential in protection of potato to new aggressive strains of PVYN. 相似文献
12.
Fattouma Djilani-Khouadja Laurent Glais Michel Tribodet Camille Kerlan Hatem Fakhfakh 《European journal of plant pathology / European Foundation for Plant Pathology》2010,126(4):479-488
Surveys were conducted of symptomatic potato plants in late season crops, from the major potato production regions in Northern
Tunisia, for infection with six common potato viruses. The presence of Potato leafroll virus (PLRV), Potato virus Y (PVY), Potato virus X (PVX), Potato virus A (PVA), Potato virus S (PVS) and Potato virus M (PVM) was confirmed serologically with virus infection levels up to 5.4, 90.2, 4.3, 3.8, 7.1 and 4.8%, respectively. As PVY
was prevalent in all seven surveyed regions, further biological, serological and molecular typing of 32 PVY isolates was undertaken.
Only one isolate was shown to induce PVYO-type symptoms following transmission to tobacco and to react only against anti-PVYO-C antibodies. Typical vein necrosis symptoms were obtained from 31 samples, six of which reacted against both anti-PVYN and anti-PVYO-C antibodies showing they contained mixed isolates, while 25 of them reacted only with anti-PVYN antibodies. An immunocapture RT-PCR molecular test using a PVYNTN specific primer pair set in the 5’NTR/P1 genomic region and examination of recombinant points in three genomic regions (HC-Pro/P3,
CI/NIa and CP/3’NTR) showed that all 25 serotype-N PVY isolates were PVYNTN variants with similar recombinations to the standard PVYNTN-H isolate. This is the first report of the occurrence of the
PVYNTN variant and its high incidence in late season potatoes in Tunisia. 相似文献
13.
Improving Potato virus Y strain nomenclature: lessons from comparing isolates obtained over a 73‐year period
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Biological and whole genome properties were compared between eight historical European (1943–1984) and five Australian (2003–2012) Potato virus Y (PVY) isolates. Based on eliciting hypersensitivity genes Nc, Ny or Nz, the former belonged to biological strain groups PVYC (CT, CRM1), PVYO (CRN, KE, RS) or PVYZ (CM2, CRM2, DS). The latter were inoculated to differential and other potato cultivars, tobacco and tomato. Two belonged to PVYO (BL, DEL3), one to PVYZ (ATL1), and one (KIP1) to suggested strain group (PVYD) which elicited putative hypersensitivity gene Nd. Tomato isolate CN1 (and unsequenced CN2), which were poorly adapted to infect potato, were not grouped. Next‐generation sequencing (NGS) of samples containing all isolates except CN2, yielded 13 complete sequences of 9592–9700 nucleotides (nt), and one partial sequence of 9002 nt, none being recombinants. Comparing the former with 60 other PVY complete genomes, found one (CRM2) in phylogenetic subgroup YO, eight in YO5 (CM2, CRN, DS, KE, RS, ATL1, BL, DEL3), three in YC2 (CRM1, CT, KIP1) and one in YC1 (CN1). Thus, biologically defined PVYO (5) and PVYZ (4) isolates were within phylogenetic subgroups YO or YO5, biological PVYC isolates (2) within YC2, biological PVYD isolate KIP1 in YC2 and tomato isolate CN1 in YC1. NGS identified KIP1 and partial sequence KIP from mixed infection. KIP was in YO5. Grouping of four PVYZ isolates within phylogenetic PVYO, and the PVYD isolate within phylogenetic PVYC, reveals disagreement between current biological and phylogenetic PVY nomenclature systems. Using Latinized numerals for phylogenetic group names resolved this. 相似文献
14.
B. Blanco-Urgoiti M. Tribodet S. Leclere F. Ponz C. Pérez de san román F.J. Legorburu C. Kerlan 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(8):811-819
A collection of 38 PVY isolates from seed potato batches, originating from several Western European countries, was characterized by using current biological, serological and molecular tools differentiating PVY strains and groups. The correlation between the three kinds of tests was good but not absolute. No single serological or PCR method was able to discriminate among the five isolate groups found. Twenty-nine isolates belonged to the PVYN strain and six to the PVYO strain. No PVYC was found. Two other isolates reacted serologically like PVYO, but were unable to elicit a hypersensitive response from the Nytbr gene and probably represent the PVYZ group. At the molecular level, these two isolates showed a combination of both PVYO and PVYN and could be recombinants of these strains. Another isolate reacted serologically like PVYO, but induced vein necrosis in tobacco, like PVYN-Wilga. Some PVYN isolates caused tuber ring necrosis in glasshouse conditions. These might belong to the PVYNTN group. The PVYNTN, PVYN-Wilga and PVYZ groups probably represent pathotypes within strains PVYN and PVYO, respectively. The present study also confirms previous reports showing a high genetic variation at the 5
end within the PVYN strain. 相似文献
15.
S. B. F. Galvino‐Costa A. dos Reis Figueira V. V. Camargos P. S. Geraldino X‐J. Hu O. V. Nikolaeva C. Kerlan A. V. Karasev 《Plant pathology》2012,61(2):388-398
Two Potato virus Y (PVY) isolates collected in Brazil, PVY‐AGA and PVY‐MON, were identified as recombinants between two parent genomes, PVYNTN and PVY‐NE‐11, with a novel type of genomic pattern. The new recombinants had an ordinary PVYNTN genome structure for approximately 6·7‐kb from the 5′‐end of the genome whereas the 3′‐terminal 3·0‐kb segment had two fragments of NE‐11‐like sequence separated by another small PVYNTN‐like fragment. PVY strains are defined based on the hypersensitive resistance (HR) response in potato indicators. Both PVY‐AGA and PVY‐MON isolates did not induce the HR in potato cultivars carrying Ny, Nc, or (putative) Nz genes and thus were able to overcome all known resistance genes to PVY. Only one of the two isolates, PVY‐AGA, induced a vein necrosis reaction in tobacco. The biological responses of the potato indicators and tobacco defined PVY‐MON as an isolate of the PVYE strain. To distinguish PVY‐AGA and PVY‐MON from other PVYNTN isolates, an RT‐PCR test was developed utilizing new specific primers from the capsid protein gene area and producing a characteristic 955‐bp band. Serological profiling of these PVY isolates with three monoclonal antibodies revealed an unusual reactivity, where one of the two commercial PVYN‐specific monoclonal antibodies did not recognize PVY‐AGA. The ability of these new PVY recombinants to overcome resistance genes in potato producing mild or no symptoms, combined with the lack of serological reactivity towards at least one PVYN‐specific antibody may present a significant threat posed by these isolates to seed potato production areas. 相似文献
16.
为明确甘薯病毒G(sweet potato virus G,SPVG)CH株系中国分离物SPVG-CH-Ch1和CH2株系中国分离物SPVG-CH2-Ch1的基因组结构特征及遗传变异情况,利用RT-PCR和RACE方法克隆获得分离物SPVG-CH-Ch1和SPVG-CH2-Ch1的基因组全序列,应用DNAMAN软件对基因组全序列及不同编码区序列进行分子变异分析,并基于多聚蛋白基因序列利用MEGA 7软件进行系统进化分析。结果表明,分离物SPVG-CH-Ch1和SPVG-CH2-Ch1的基因组分别包含10 813个和10 834个核苷酸,均包含1个开放阅读框,由10 467个核苷酸组成,编码含有3 488个氨基酸残基的多聚蛋白。分离物SPVG-CH-Ch1与SPVG-CH2-Ch1之间的基因组全序列核苷酸一致性为78.6%,二者与GenBank中登录的其它SPVG分离物基因组全序列核苷酸一致性分别为78.6%~99.1%和77.9%~98.6%,其中SPVG-CH-Ch1与IS103分离物(KM014815)的核苷酸一致性最高,为99.1%,与WT325分离物(KF790759)的核苷酸... 相似文献
17.
正甘薯病毒2(Sweet potato virus 2,SPV2)是马铃薯Y病毒科(Potyviridae)马铃薯Y病毒属(Potyvirus)成员。SPV2也称为甘薯脉花叶病毒(ipomoea vein mosaic virus,IVMV)和甘薯Y病毒(sweet potato virus Y,SPVY)~[1],是甘薯上常见的病毒之一。SPV2病毒粒体为线条状,长度为850 nm,在细胞质中形成风轮状或卷轴状内含体~[2]。SPV2可由桃 相似文献