猴头菌转化EGB发酵液提取物抗氧化及抑制非酶糖基化活性研究

[目的]研究猴头菌转化EGB发酵液提取物抗氧化及抑制非酶糖基化活性。[方法]以DPPH自由基清除率和还原力两种抗氧化能力及非酶糖基化抑制率为考察指标,比较了猴头菌转化EGB发酵液经AB-8大孔树脂吸附前后,发酵液冻干物、提取物抗氧化及抑制非酶糖基能力的变化。[结果]发酵液经AB-8大孔树脂吸附后可浓缩分离活性物质,吸附后的提取物自由基清除率、还原力、非酶糖基化抑制率均显著提高。提取物中可能含有三萜皂甙类和黄酮类成分。[结论]AB-8树脂可用于猴头菌转化EGB发酵液提取物的初步浓缩。     

猴头菌转化EGB发酵液提取物抗氧化及抑制非酶糖基化活性研究

猴头菌转化银杏叶粗提物发酵液具有清除体外自由基及抑制体外非酶糖基化反应的作用，对四氧嘧啶诱导的糖尿病模型小鼠具有显著的降血糖效果。但由于发酵液成分复杂，有效成分含量低，不便于直接服用，因而有必要对发酵液功能成分进行提取。笔者以抗氧化活性、体外非酶糖基化反应为指标，比较了、猴头菌转化EGB发酵液经AB-8吸附前后活性物质的活性变化，以期为发酵液的分离提取及提取产物的利用提供参考。     

黑穗醋栗多糖抗氧化及抑制非酶糖基化活性的研究

从清除超氧阴离子自由基、清除羟自由基、清除DPPH自由基3个方面研究黑穗醋栗多糖体外抗氧化活性,VC作对比,并通过NBT还原试验、二羰基化合物测定试验、非酶糖基化终产物荧光性测定试验,分别测定黑穗醋栗多糖对糖基化的干预作用,以明确黑穗醋栗多糖体外抗氧化作用及抑制非酶糖基化能力.当黑穗醋栗多糖浓度为1.0 mg·mL-1时,对DPPH清除率为46.20%,对OH·,O2-·的清除能力较强,其IC50分别为0.976和0.3931 mg·mL-1,对非酶糖基化反应3个阶段的抑制率分别为34.78%、30.80%和43.14%.结果表明黑穗醋栗多糖具有抗氧化活性,且抗氧化能力随浓度的增加而增强,呈量效关系,同时也表现出一定的抑制非酶糖基化的活性.     

黑穗醋栗总黄酮的抗氧化及抗非酶糖基化活性研究

文章考查黑穗醋栗总黄酮对羟基自由基(.OH)、超氧阴离子自由基(O2-.)以及DPPH自由基的清除能力,并通过NBT还原实验、二羰基化合物测定实验、非酶糖基化终产物的荧光性测定试验,研究黄酮对非酶糖基化反应三个阶段的抑制作用。结果表明,在黄酮浓度为1.0 mg.mL-1时,对羟自由基的清除率达到82.46%;浓度为0.8 mg.mL-1时,对超氧阴离子自由基的清除率达到81.33%;浓度为0.6 mg.mL-1时,对DPPH自由基清除率达到57.68%;对非酶糖基化反应三个阶段的抑制率分别为30.69%、48.15%、42.59%;黑穗醋栗总黄酮作为一种天然自由基清除剂,具有良好的抗氧化活性,同时也表现出一定的抗非酶糖基化活性。     

荞麦提取物抗氧化活性研究

以不同生育时期的甜荞和苦荞乙醇提取物为研究对象,在测定其提取物中总酚和总黄酮含量的基础上,利用体外法研究了荞麦提取物的总抗氧化性及对羟基自由基的清除作用。结果表明,参试品种总酚和总黄酮含量在整个生育期变化趋势一致,呈先升高后降低的变化趋势,在盛花期达到最高,苦荞含量高于甜荞,达0.05显著水平;两品种的乙醇提取物均有显著的抗氧化性,与甜荞相比,苦荞的总抗氧化活性以盛花期最强,羟基自由基的清除作用也最强,清除率达57.1%;回归分析表明,荞麦的总酚含量与总抗氧化性及对羟基自由基的清除率呈线性相关,相关系数可达0.90以上。     

荞麦提取物抗氧化活性研究

[目的]研究荞麦不同部位提取物抗氧化活性差异,更好地开发荞麦的保健功能。[方法]分别考察荞麦皮、荞麦粉和荞麦粒乙醇提取物对DPPH自由基和ABTS自由基的清除作用,以及其黄酮类化合物的得率。[结果]三者乙醇提取物中黄酮类化合物得率最高的为荞麦皮,最小的为荞麦粉,荞麦粒介于两者之间;三者提取物对DPPH自由基和ABTS自由基均具有很好的清除效果,且对DPPH自由基和ABTS自由基清除率达50%的样品浓度比较,荞麦皮浓度最低,荞麦粉最高,荞麦粒浓度介于两者之间。[结论]荞麦中黄酮类化合物是荞麦提取物具有抗氧化活性作用的重要成分。     

葛根提取物抗氧化活性研究

[目的]研究葛根提取物对.OH和清除作用。[方法]采用邻二氮菲-Fe2+氧化法和邻苯三酚自氧化法对葛根水提取物和乙醇提取物及葛根素对清除.OH和作用的效果进行了研究。[结果]葛根提取物对.OH和都有一定的清除作用,其清除两种自由基的能力为葛根素>葛根水提物>葛根醇提物。当葛根水提物及葛根素浓度为50 mg/ml,乙醇提物为75 mg/ml时,对.OH清除率分别为40.69%、27.48%和14.66%。3种提取物对清除作用都是在一定浓度后呈现出明显剂量-效应关系。[结论]葛根提取物具有较强的抗氧化作用,能增强机体防御自由基损伤的能力,该研究结果为葛根提取物特别是葛根素在心脑血管疾病治疗提供了有力的佐证。     

两粤黄檀体外抗氧化及α-葡萄糖苷酶抑制活性研究

研究了两粤黄檀茎不同溶剂(95%乙醇、丙酮、乙酸乙酯)提取物的抗氧化和α-葡萄糖苷酶抑制活性。采用清除DPPH自由基、清除ABTS+自由基和铁离子(Fe2+)还原能力检测体外抗氧化活性;体外建立酶反应体系,以pNPG作为底物,检测其α-葡萄糖苷酶抑制活性。结果表明,两粤黄檀茎乙酸乙酯提取物具有优异的抗氧化能力及α-葡萄糖苷酶抑制活性,两粤黄檀茎乙酸乙酯提取物具有作为天然抗糖尿病药物研究和开发的潜力。     

桑叶黄酮抗氧化及抑制蛋白糖基化作用

为观察桑叶总黄酮的降糖作用及其对抗氧化酶、蛋白糖基化的影响.用桑叶总黄酮(8 g生药/kg体重)灌胃四氧嘧啶诱导的糖尿病大鼠4周,观察给药前后血糖变化,测定LPO、SOD、GSH-px及果糖胺水平;分别以0.001,0.01,0.1 g/L桑叶总黄酮浓度加入体外蛋白糖基化体系孵育1,4,7,14,21,28d,测定早期糖化蛋白产物的浓度.结果表明:与对照组比较,桑叶总黄酮组有明显降血糖作用.桑叶总黄酮组血糖由(18.33±3.33)mmol/L降至(10.19±4.45)mmol/L,P＜0.05,LPO含量由(10.51±2.31)nmol/L,降至(6.01±2.04)nmol/L,P＜0.05,SOD水平由(471.1±103.0)Nu/mL,升至(678.7±31.5)Nu/mL,P＜0.001,果糖胺水平由(6.22±1.37)mmol/L,降至(1.70±0.32)mmol/L,P＜0.05,体外糖化蛋白产量孵育时间呈正相关,0.1 g/L桑叶总黄酮在各时间点均能使糖化蛋白生成量降低.因此:桑叶总黄酮能显著降低糖尿病大鼠血糖、LPO含量并提高SOD水平,抑制蛋白糖基化.     

首乌提取物抑制人血清蛋白糖基化反应研究

[目的]研究首乌提取物对人血清蛋白糖基化的抑制效果。[方法]选择不同浓度的首乌提取物混入人血清蛋白-葡萄糖反应体系中得到不同的糖基化产物。[结果]加入首乌提取物抑制剂的糖基化人血清蛋白的自由氨基含量减少缓慢，空间结构发生改变，疏水基团也不易暴露；从抗氧化性角度而言，ABTS⁺·、DPPH·、超氧阴离子自由基清除能力和还原能力均降低，说明糖基化后期产物生成量减少。[结论]该研究为首乌提取物在人类医药健康领域的开发利用提供理论依据。     

Study on Anti-oxidation and Inhibitory Effect on Nonenzymatic Glycation Reaction of Fermentation Extract from Biotransformation of Ginkgo biloba L. (EGB) by Hericium erinaceus

[Objective] In order to study the anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of EGB fermentation extraction biotransformed by Hericium erinaceus.[Method]The free radical scavenging ability and reducing capacity of DPPH as well as inhibitory rate of nonenzymatic glycation reaction were measured targets for comparing changes of anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of fermentation lyophilizer and fermentation extraction before and after EGB fermention adsorbed by AB-8 macroporous resin. The EGB fermention was biotransformed by Hericium erinaceus.[Result]After adsorbed by AB-8 macroporous resin,the bioactive matters were concentrated and separated. The free radical scavenging rate,reducing capacity and inhibitory rate of nonenzymatic glycation reaction were increased significantly after adsorbed by AB-8 macroporous resin.[Conclusion]AB-8 macroporous resin could be used for preliminary concentration of EGB fermentation which was biotransformed by Hericium erinaceus.     

Study on Anti-oxidation and Inhibitory Effect on Nonenzymatic Glycation Reaction of Fermentation Extract from Biotransformation of Ginkgo biloba L. (EGB) by Hericium erinaceus

[Objective] In order to study the anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of EGB fermentation extraction bio-transformed by Hericium erinaceus. [Method] The free radical scavenging ability and reducing capacity of DPPH as well as inhibitory rate of nonenzymatic glycation reaction were measured targets for comparing changes of anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of fermentation ly-ophilizer and fermentation extraction before and after EGB fermention adsorbed by AB-8 macroporous resin. The EGB fermention was biotransformed by He-ricium erinaceus. [Result]After adsorbed by AB-8 macropous resin,the bioactive matters were concentrated and separated. The free radical scavenging rate, reducing capacity and inhibitory rate of nononzymatic glycation reaction were increased significantly after adsorbed by AB-8 macroporons resin. [Con-dusion] AB-8 macroporous resin could be used for preliminary concenuation of EGB fermentation which was biotransfonned by Hericium erinaceus.     

银杏叶黄酮的酶法提取及生物转化研究

研究了银杏叶黄酮的酶法提取及生物转化工艺。结果证明,酶法提取能够显著提高银杏叶黄酮提取率,生物转化能够显著提高银杏叶黄酮中苷元黄酮的含量。     

不同方法提取银杏果实中黄酮类化合物的研究

利用乙醇法和超声波法对银杏果实中黄酮类化合物进行提取,测定黄酮提取率,在单因素试验基础上分别设计L9(34)和L9(33)正交试验,获得各自最佳提取条件.结果表明,利用乙醇法提取的最佳条件是:70%乙醇作提取剂,料液比为1 g :9 mL,于60 ℃下提取3次,黄酮提取率为0.522%;超声波法提取最佳条件为:70%乙醇作提取剂,料液比1 g :34 mL,提取60 min,黄酮提取率为0.75%.黄酮不同提取方法比较,超声波法优于乙醇法.     

NaOH溶液提取银杏叶总黄酮方法的研究

采用单因素试验及交互试验对NaOH溶液提取银杏叶总黄酮的条件进行了优选。结果表明:NaOH浓度、浸提温度、浸提时间、提取次数及固液比对银杏叶总黄酮的提取率均有影响,最佳提取工艺为:用50倍(V/W)的0.25%NaOH溶液于70℃条件下提取2次、每次0.5 h,在该工艺条件下银杏叶总黄酮提取率可达1.264%。     

孑遗植物银杏（Ginkgo bilobaL．）雌雄株水分生理特征研究

1材料与方法1．1生境及其自然环境试验地位于四川盆地西缘的四川省都江堰市灵岩山,为山地。研究地点地处31°01’N,103°36＇E,气候属中亚热带湿润气候区,年均温为12．5℃。1月平均气温4．6℃,7月平均气温24．7℃。降水充沛,年均降雨量为1200-1800mm,云雾多、日照少,年均日照时数900h,年均相对湿度为75％-80％。     

孑遗植物银杏（Ginkgo biloba L．）雌雄株水分生理特征初步研究

[目的]研究银杏雌雄株在水分生理上的差异,探讨其水分利用策略,以及在进化过程中的重要作用。[方法]对银杏雌雄株的树干液流（sap flow）、气孔导度（Gs）、蒸腾速率（Tr）以及水分利用效率（WUE）进行对比研究。[结果]雌雄银杏的液流昼夜进程具有相似的规律,且白天雌雄株液流基本相同,夜间雄株液流大于雌株;不同性别银杏的丹和岱同样呈现为早晚高、午间低的曲线趋势,早晚雌株有比雄株更高的Tr和Gs,但中午11：00-14：00之间雌株的值低于雄株;雌雄银杏的WUE曲线变化趋势相似,但雌株的平均水分利用效率略小于雄株。[结论]与其他伴性植物比较分析得知,不同性别银杏的水分生理有较强的趋同特征。这可能是在长期进化的压力下,雌雄异株植物的一种生存策略。     

孑遗植物银杏(Ginkgo biloba L.)雌雄株水分生理特征研究(英文)

[Objective] The experiment aimed to study the difference of water physiology of male and female Ginkgo biloba L. for discussing the strategy of water utilization as well as the important role of this difference during evolution process. [Method] The stem sap flow, stomatal conductance(Gs), transpiration rate(Tr) and water use efficiency (WUE) of male and female Ginkgo biloba L. were comparatively studied. [Result] The day-night processes of flow on male and female Ginkgo biloba L. were similar. The flow on male and female Ginkgo biloba L. in day were almost same while the flow at night on male Ginkgo biloba L. was bigger than that on female Ginkgo biloba L. The Tr and Gs of male and female Ginkgo biloba L. were high in morning and at night but low at noon ,while Tr and Gs of female Ginkgo biloba L. in morning and at night were higher than these of male Ginkgo biloba L. at the same time point. However, these indexes of female plant were lower than these of male plant from 11:00 to 14:00. WUE changing trends of male and female Ginkgo biloba L. were similar, while average water utilization rate of female Ginkgo biloba L. was slightly lower than that of male Ginkgo biloba L. [Conclusion] Compared with other companion plants, water physiology of male and female Ginkgo biloba L. had strong homoplasy. The phenomenon might be a survival strategy of dioecious plants under long term evolutionary pressure.