不同冷链物流过程对金枪鱼品质及组织形态的影响

为研究物流过程中温度变化对金枪鱼品质及组织形态的影响,试验模拟5种不同物流过程,测定金枪鱼肉感官、色差、持水力、质构以及肌纤维间隙。结果表明,超低温冻藏大目金枪鱼肉的品质受物流过程中温度变化影响显著,储运、销售过程始终保持在-55℃的完整冷链下能够使金枪鱼肉保持良好品质;随着储运过程中温度变化次数增多、幅度增大,鱼肉劣化显著,其中,经2次-18℃运输的金枪鱼肉在冻藏末期组织松散、肌纤维间隙达17.20μm,在4℃家用冰箱中贮藏12 h后感官评分、红度值、持水力、硬度和咀嚼性分别为-0.81、5.2、53.08%、1.128 N和58.03,其质量甚至低于物流过程始终保持在-18℃而不产生温度变化的鱼肉。若运输过程不具备超低温冷藏车,仅靠超低温贮藏并不能减缓鱼肉品质劣变,此时应适当调节贮藏温度,尽量避免储运期间的温度变化;相较于2℃冷藏销售,-18℃冷冻柜销售能够使金枪鱼肉保持较好品质。研究结果可为金枪鱼储运和销售提供参考。     

Use of real-time polymerase chain reaction (PCR) and transformation assay to monitor the persistence and bioavailability of transgenic genes released from genetically modified papaya expressing nptII and PRSV genes in the soil

Soil samples were collected from an isolated field from December 2003 to April 2004 where transgenic papaya were planted, and the persistences of transgenic genes of 796 bp (located between 35S promoter and coat protein, 35S-P/PRSV-CP), 398 bp (located between plasmid pBI121 and NOS terminator, pBI121/NOS-T), and 200 bp (located between NOS promoter and nptII gene, NOS-P/nptII) were studied. At the end of planting, the residues of 398 bp in the soil was 0.06 microg g(-1) of soil, whereas the residues of 769 and 200 bp were less than 30 pg g(-1) of soil (detection limit). Kinetics studies on the persistence of these three fragments in sterile distilled water and nonsterile soil microcosms showed that two mechanisms might be involved: an initial fast exponential degradation pattern in the first week and then followed by a slow-release pattern throughout the experiment. Persistence of transgenic DNA in sterile water was longer than in nonsterile soil microcosms, indicating that enzymatic degradation and soil adsorption played important roles on the persistence of DNA in the environment. The reason for the fragment of 398 bp persisted longer than fragments of 769 and 200 bp is not clear, but the guanine plus cytosin (G plus C) content in the DNA fragment might be involved in the stability of DNA in the environment. Biological availability of soil DNA to bacteria conducted by the transformation assay indicated that gene transformation from soil DNA extracts to two Acinetobacter spp. did not occur.