True ileal amino acid digestibility and endogenous ileal amino acid losses in growing pigs fed wheat shorts- or casein-based diets

Use of dietary AA in growing pigs reflects digestion and use of digested AA for various body functions. Before evaluating dietary effects on use of digestible AA intake for body protein deposition, a digestibility study was conducted to investigate true ileal AA digestibility and endogenous ileal AA losses in growing pigs fed graded levels of wheat shorts (WS) or casein (CS; control). A casein-based basal diet (basal) was formulated to contain 0.27 g of standardized ileal digestible (SID) Lys per MJ of DE, to which extra Lys was added from WS (WS2, +0.10 g of SID Lys per MJ of DE; WS3, +0.20 g of SID Lys per MJ of DE) or casein (CS3, +0.20 g of SID Lys per MJ of DE). A fifth diet was formulated to be similar in CP level and source as CS3 but in which 6% pectin, a source of soluble non-starch polysaccharides (NSP), was included at the expense of cornstarch (CS3 + pectin). Five Yorkshire barrows (17.5 +/- 1.5 kg of BW) were fitted with a T-cannula at the distal ileum and randomly assigned to 1 of the 5 experimental diets in a 5 x 5 Latin Square design. Apparent ileal digestibility (AID), true ileal digestibility (TID), and endogenous ileal protein losses (EPL) were determined using the homoarginine method. Diet CS level did not influence (P > or = 0.10) TID of most essential AA or EPL (10.4 g/kg of DM intake). Including pectin in the diet did not influence TID of AA (P > or = 0.10) but increased EPL (15.6 g/kg of DM intake; P > or = 0.01). Inclusion of WS in the diet reduced TID of most essential AA (P < 0.01). The TID values for most essential AA, however, were the same (P > or = 0.10) for both dietary WS levels, except for Lys and Met, which were further reduced at the greatest dietary WS level. Increased EPL (P < 0.01) was only observed for WS3 (16 g/kg of DMI). We concluded that (1) the effects of dietary protein source on AID of AA can be attributed both to reduced TID of AA and increased EPL, (2) the impact of dietary WS level on TID of AA and EPL does not seem to be linear, (3) soluble NSP from pectin or WS exerts a greater effect on EPL than insoluble NSP, and (4) because of the metabolic cost associated with EPL and the impacts of feed composition on microbial fermentation in the gut lumen, the effects of feed ingredients on the use of ileal digestible AA for protein deposition should be investigated further.     

Effects of body weight and feed intake level on basal ileal endogenous losses in growing pigs

An experiment was carried out to determine the effects of feeding level, body weight, and time after surgery on basal ileal endogenous amino acid (AA) and N losses in growing pigs. Three pairs of littermate pigs were surgically prepared with ileo-rectal anastomoses. One pig in each pair was anastomosed at 38 kg BW, and the remaining pigs were anastomosed at 67 kg BW. Each pig received at different periods 50, 70, or 90 g of dry matter per kilogram of BW.75 of a protein-free diet according to a Latin square design involving three pigs starting at 45 kg BW and involving six pigs starting at 77 kg BW. For most AA, the time after surgery x feeding level interaction was significant. The basal endogenous losses (in g/d) increased linearly with feeding level at both BW. At the higher BW, the basal endogenous losses (in g/kg DMI) were constant regardless of feeding level, whereas at the lower BW they responded quadratically. At the low feeding level, the endogenous losses were higher than at the medium or high feeding level. We concluded that the basal endogenous losses are proportional to DMI when the feeding level is higher than 70 g/kg BW.75. The AA profile was not influenced by these three variables, but there was a large animal effect. These results suggest that, in digestibility trials, an assessment of the basal ileal endogenous AA losses must be performed on each pig to correct the apparent ileal AA digestibility data.     

Standardized ileal protein and amino acid digestibility by growing pigs and sows

Experiments were conducted to determine the effect of the physiological condition of swine on standardized ileal digestibility coefficients (SID). The apparent ileal digestibility coefficients were determined for crude protein and amino acids in six feed ingredients (corn, barley, wheat, soybean meal, canola meal, and meat and bone meal) in growing pigs and in gestating and lactating sows. Growing pigs and lactating sows were given free access to their diets, whereas gestating sows were allowed to consume only 2 kg of feed daily. The nonspecific (basal) endogenous losses of protein and amino acids were determined under similar feeding regimens after feeding a protein-free diet. The SID for crude protein and amino acids were calculated by correcting the apparent ileal digestibility coefficients for the nonspecific endogenous losses of protein and amino acids. With a few exceptions, there were no differences (P > 0.05) in the SID for crude protein and amino acids between growing pigs and lactating sows. Overall, gestating sows had higher (P < 0.05) SID for crude protein and all amino acids, except for tryptophan and aspartate, compared with growing pigs. Likewise, the SID of most amino acids obtained by gestating sows were higher (P < 0.05) than those obtained by lactating sows. Interactions (P < 0.05) between animals and diets were observed for gestating sows compared with growing pigs as well as gestating sows compared with lactating sows. As a consequence, it is not possible to extrapolate data from one feed ingredient to another. On most occasions, the lowest SID among the indispensable amino acids was calculated for threonine, valine, and lysine. It is concluded that gestating sows fed 2 kg of feed per day have higher standardized digestibility coefficients than do growing pigs and lactating sows given free access to their diets. This difference may be due to differences in daily feed intake rather than to the physiological status of the animals.     

Determination of basal ileal endogenous losses and standardized ileal digestibility of amino acids in barley fed to growing pigs

Background: Basal ileal endogenous amino acid(AA) losses(IAA_(end)) and standardized ileal digestibility(SID) values of cereal grains, such as barley, are apparently underestimated when determined according to the nitrogen(N)-free method. Regression analysis between the dietary apparent ileal digestible content(c AID) and total crude protein(CP) and AA can be considered as alternative approach to obtain more accurate values for IAA_(end)and SID of AA in cereal grains.Methods: Eight hulled barley genotypes were used, with barley being the only source of CP and AA in the assay diets. The diets contained 95 % as-fed of these eight barley genotypes each, ranging in CP content between 109.1 and 123.8 g/kg dry matter(DM). Nine ileally T-cannulated barrows, average body weight(BW) 30 ± 2 kg, were allotted to a row-column design comprising eight periods with 6 d each and nine pigs. On d 5 and the night of d 6 of every period, ileal digesta were collected for a total of 12 h. The IAA_(end) and the SID were determined by linear regression analysis between c AID and total dietary CP and AA.Results: There exist linear relationships between cA ID and total CP and AA(P 0.001). The IAA_(end) of CP, Lys, Met, Thr and Trp amounted to 35.34, 1.08, 0.25, 1.02 and 0.38 g/kg DM intake(DMI), respectively, which are greater compared to average IAA_(end) determined previously under N-free feeding conditions. The SID of CP, Lys, Met, Thr and Trp was 90,79, 85, 79 and 86 %, respectively, and was greater when compared to tabulated values. Moreover, these SID values were greater than those reported in literature, based on correction of apparent ileal digestibility(AID) of CP and AA for their IAA_(end)values. Summarized, the results of the present regression analysis indicate greater IAA_(end)in barley-based diets compared to those obtained by N-free feeding.Conclusions: For low-protein feed ingredients like barley the regression method may be preferred over correction of AID values for their IAA_(end)determined under N-free feeding conditions, as intercepts and slopes of the linear regression equations between cA ID and total dietary CP and AA provide direct estimates of IAA_(end)and SID of CP and AA in the presence of the assay feed ingredient.     

水飞蓟复合饲料对猪生长性能及日粮养分消化率的影响

选择36只平均重25kg左右的杜×长×大三元杂交猪,按体重和性别随机分为2组,每组3个重复,每个重复6只。在试验组中应用水飞蓟药物残渣作为其主要饲料成分,并添加中草药复合制剂,对照组为抗生素组,研究对猪生长性能和饲料利用率的影响。结果表明:水飞蓟组在提高日增重和日粮养分消化率方面与抗生素组没有明显差异,但试验组经济效益明显。     

Neutral detergent fiber increases endogenous ileal losses but has no effect on ileal digestibility of amino acids in growing pigs

Two experiments were conducted to determine the effect of neutral detergent fiber (NDF) on endogenous amino acids and protein ileal losses; and also apparent ileal digestibility (AID), and standardized ileal digestibility (SID) of amino acids and crude protein. Sixteen barrows were fed four protein‐free diets containing graded NDF levels in Experiment 1. NDF was a mixture of sugarcane bagasse and corn leaves (SBCL). Twenty‐four barrows were fed diets with soybean protein concentrate (SPC) or casein as protein sources and SBCL or corncobs (CC) as NDF sources in Experiment 2. In Experiment 1, a linear increase (P < 0.05) in endogenous amino acid and protein ileal losses was observed with increased NDF levels, except for arginine, histidine, methionine and proline. In Experiment 2, protein (P < 0.001) and NDF (P < 0.01) sources significantly affected AID of dry matter, which was higher in casein diets (71.7%) and CC diets (70.7%). Protein and NDF sources significantly affected (P < 0.05) SID of crude protein, which was higher in casein diets (92.8%) and CC diets (92.7%). NDF source had no effect (P > 0.05) on SID of amino acids. Overall, this study showed that NDF increased endogenous amino acid and protein ileal losses, but did not affect ileal digestibility of amino acids.     

The effect of grinding and pelleting of wheat and maize on amino acids true ileal digestibility and endogenous losses in growing pigs

Two experiments were performed in order to test the effects of grinding and pelleting wheat (experiment 1) or maize (experiment 2) on amino acids (AA) standardized (SID) and true (TID) ileal digestibility. Basal ileal endogenous losses (IEL), used to calculate SID from apparent digestibility, were determined by feeding a protein-free diet. Total IEL required to calculate TID were estimated as the difference between total and dietary losses determined according to the isotope dilution technique using ¹⁵N-labeled crops. Wheat and maize were ground to compare two different mean particle sizes (d₅₀ 500 μm and 1000 μm). Then, a fraction of the 500-μm mash was further pelleted through two dies of different thicknesses (16 vs. 20 mm for wheat and 16 vs. 24 mm for maize), holes diameter 4 mm in both cases. Cereals were incorporated in 4 isoproteic diets either in mash form (fine vs. coarse mash) or in pelleted form (thin vs. thick die). In experiment 1, wheat was associated with rapeseed meal diets, while in experiment 2, maize was associated with soybean meal. The reduction of wheat particle size affected neither AA SID nor TID. In contrast, the pelleting treatment significantly improved protein and most AA SID and TID. In addition, total IEL of protein and several AA were significantly reduced when the thicker die was used. Numeric improvements of protein and AA SID were observed with reduction of maize particle size and this was associated with a significant decrease in total IEL so that TID was not modified. Further improvements of AA SID and TID occurred with pelleting of maize, without significant effect on IEL regardless of die thickness.     

Novel procedure for estimating endogenous losses and measurement of apparent and true digestibility of phosphorus by growing pigs

An experiment was conducted to evaluate a novel procedure for estimating endogenous losses of P and for measuring the apparent total tract digestibility (ATTD) and true total tract digestibility (TTTD) of P in 5 inorganic P sources fed to growing pigs. The P sources were dicalcium phosphate (DCP), monocalcium phosphate (MCP) with 50% purity (MCP50), MCP with 70% purity (MCP70), MCP with 100% purity (MCP100), and monosodium phosphate (MSP). A gelatin-based, P-free basal diet was formulated and used to estimate endogenous losses of P. Five P-containing diets were formulated by adding 0.20% total P from each of the inorganic P sources to the basal diet. A seventh diet was formulated by adding 0.16% P from MCP70 to the basal diet. All diets were fed to 7 growing pigs in a 7 x 7 Latin square design, and urine and feces were collected during 5 d of each period. The endogenous loss of P was estimated as 139 +/- 18 mg/kg of DMI. The ATTD of P in MSP was greater (P < 0.05) than in DCP, MCP50, and MCP70 (91.9 vs. 81.5, 82.6, and 81.7%, respectively). In MSP, the TTTD of P was 98.2%. This value was greater (P < 0.05) than the TTTD of P in DCP, MCP50, and MCP70 (88.4, 89.5, and 88.6%, respectively). The ATTD and the TTTD for MCP70 were similar in diets formulated to contain 0.16 and 0.20% total P. Results from the current experiment demonstrate that a P-free diet may be used to measure endogenous losses of P in pigs. By adding inorganic P sources to this diet, the ATTD of P can be directly measured and the TTTD of P may be calculated for each source of P.     

Amino acid and energy digestibility of protein sources for growing pigs

Two experiments were conducted to determine the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of AA and DE, and to estimate ME and NE of rice protein concentrate, salmon protein hydrolysate, whey protein concentrate, and spray-dried plasma protein. In Exp. 1, 6 barrows (initially 29.5 +/- 2.5 kg of BW) were fitted with ileal T-cannulas and fed each of 5 cornstarch-based diets in a balanced crossover design over 35 d. During a given week, there were either 1 or 2 replications of each treatment, resulting in 6 total replications over 5 wk. The 4 test diets (fed from d 0 to 28) were formulated to contain 12.5% CP by using analyzed nutrient compositions of rice protein concentrate, salmon protein hydrolysate, whey protein concentrate, or spray-dried plasma protein. The fifth (N-free) diet was fed from d 28 to 35 to estimate basal endogenous losses of CP and AA, which were used to calculate SID. Ileal digesta were collected and analyzed, and AID and SID values were calculated. Apparent ileal digestible Lys, Met, and Thr values were 80.0 +/- 3.3, 65.6 +/- 3.1, and 68.4 +/- 4.5% for rice protein concentrate; 85.6 +/- 4.8, 85.5 +/- 4.3, and 69.8 +/- 8.5% for salmon protein hydrolysate; 93.3 +/- 1.4, 89.9 +/- 5.8, and 83.6 +/- 5.3% for whey protein concentrate; and 92.8 +/- 0.9, 85.7 +/- 2.1, 86.5 +/- 2.3% for spray-dried plasma protein, respectively. In Exp. 2, 6 barrows (initially 37.6 +/- 1.7 kg of BW) were fed each of 5 corn-based diets in a balanced crossover design over 35 d. During a given week, there were either 1 or 2 replications of each treatment, resulting in 6 total replications over 5 wk. The 4 diets containing the test ingredients were formulated to contain approximately 20% CP by using their analyzed nutrient compositions. The fifth (corn control) diet containing 8.2% CP was also used to calculate energy values by difference. Feces were collected to determine DE. The ME and NE contents were estimated using published regression equations. The DE, ME, and NE (as-fed) values were 4,724 +/- 461, 4,226 +/- 437, and 3,235 +/- 380 kcal/kg for rice protein concentrate; 4,173 +/- 1,052, 3,523 +/- 1,002, and 2,623 +/- 872 kcal/kg for salmon protein hydrolysate; 4,949 +/- 1,002, 4,352 +/- 955, and 3,344 +/- 831 kcal/kg for whey protein concentrate; and 4,546 +/- 673, 3,979 +/- 652, and 3,020 +/- 567 kcal/kg for spray-dried plasma protein, respectively. The excellent AA digestibility and relatively high DE, ME, and NE values indicate that these protein sources warrant further investigation as ingredients for growing pig diets.     

Chemical composition,energy and amino acid digestibility in double-low rapeseed meal fed to growing pigs

Background:The nutritional value of rapeseed meal may be variable due to the variation of its chemical composition.And a precise understanding of the nutritional value of an ingredient is beneficial for the accurate diet formulation and reduction of feed costs.This study was conducted to determine the chemical composition,digestible energy(DE) and metabolizable energy(ME) content,and apparent ileal digestibility(AID) and standardized ileal digestibility(SID) of amino acids(AA) for growing pigs.Thirteen solvent-extracted double-low rapeseed meal(DLRSM) samples were obtained from the main double-low rapeseed producing areas in China.Methods:The DE and ME contents of the 13 DLRSM samples were measured in growing pigs(six pigs per DLRSM sample,average initial body weight(BW) = 48.3 kg).The AID and SID of AA of 10 DLRSM samples were determined in 12 crossbred barrows(average initial BW = 35.3 kg) by using two 6x6 Latin square designs.Each Latin square comprised one N-free diet and 5 DLRSM test diets.Results:The chemical composition of DLRSM varied among samples,and the coefficient of variation was greater than 10%for ether extract(EE),neutral detergent fiber(NDF),acid detergent fiber(ADF),calcium(Ca),and total glucosinolates.The AA content of DLRSM varied among samples especially for lysine(Lys) and methionine(Met).On a dry matter(DM) basis,the apparent total tract digestibility(ATTD) of gross energy(GE),the DE and ME and the ME:DE ratio of DLRSM averaged 62.39%,2862 kcal/kg and 2723 kcal/kg,and 94.95%,respectively.The mean value of SID of Lys was 70.52% which varied from 66.54-76.54%.The SID of crude protein(CP),Met,and threonine(Thr) averaged 72.81%,82.41%,and 69.76%,respectively.Conclusions:There was great variability in chemical composition especially in the concentration of EE,NDF and ADF,but no significant differences in energy content of the DLRSM samples were observed.In addition,the AID and SID of all AA were relatively similar among DLRSM samples except for that of Lys.     

Chemical composition,energy and amino acid digestibility in double-low rapeseed meal fed to growing pigs

Background

The nutritional value of rapeseed meal may be variable due to the variation of its chemical composition. And a precise understanding of the nutritional value of an ingredient is beneficial for the accurate diet formulation and reduction of feed costs. This study was conducted to determine the chemical composition, digestible energy (DE) and metabolizable energy (ME) content, and apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) for growing pigs. Thirteen solvent-extracted double-low rapeseed meal (DLRSM) samples were obtained from the main double-low rapeseed producing areas in China.

Methods

The DE and ME contents of the 13 DLRSM samples were measured in growing pigs (six pigs per DLRSM sample, average initial body weight (BW) = 48.3 kg). The AID and SID of AA of 10 DLRSM samples were determined in 12 crossbred barrows (average initial BW = 35.3 kg) by using two 6 × 6 Latin square designs. Each Latin square comprised one N-free diet and 5 DLRSM test diets.

Results

The chemical composition of DLRSM varied among samples, and the coefficient of variation was greater than 10 % for ether extract (EE), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium (Ca), and total glucosinolates. The AA content of DLRSM varied among samples especially for lysine (Lys) and methionine (Met). On a dry matter (DM) basis, the apparent total tract digestibility (ATTD) of gross energy (GE), the DE and ME and the ME:DE ratio of DLRSM averaged 62.39 %, 2862 kcal/kg and 2723 kcal/kg, and 94.95 %, respectively. The mean value of SID of Lys was 70.52 % which varied from 66.54–76.54 %. The SID of crude protein (CP), Met, and threonine (Thr) averaged 72.81 %, 82.41 %, and 69.76 %, respectively.

Conclusions

There was great variability in chemical composition especially in the concentration of EE, NDF and ADF, but no significant differences in energy content of the DLRSM samples were observed. In addition, the AID and SID of all AA were relatively similar among DLRSM samples except for that of Lys.     

Individual response of growing pigs to sulphur amino acid intake

Two N balance experiments were conducted to study the individual response of growing pigs to limiting amino acid (AA) intake. Series of fifteen diets with increasing concentration of sulphur amino acids (SAA, Expt 1) or methionine in the presence of excess cystine (Expt 2) were fed sequentially to nine pigs during a 15-day experimental period. The concentration of the AA under test ranged from 50% to 140% of the requirement while other essential AA were given in a 25% excess relative to the limiting AA. N retention was related to the limiting AA intake using rectilinear and curvilinear models. In Expt 1, the quadratic-plateau model fitted the individual data significantly better (p = 0.01) than the linear-plateau model. No difference was found between the two models in Expt. 2, presumably due to the sparing effect of excess cystine on methionine utilization. Exponential, saturation kinetics or four-parameter logistic models fitted to data for all pigs showed that their goodness of fit was similar to those of quadratic-plateau or linear-plateau models. Significant differences (p < 0.05) were found between individual plateau values for N retention within each experiment while the slopes of the regression lines did not significantly differ either in Expt 1 (p = 0.07) or Expt 2 (p = 0.45). There was a positive correlation between the slope and plateau values of the linear-plateau model in Expt 1 (r = 0.74, p = 0.02) but no significant correlation was found in Expt 2 (r = -0.48, p = 0.13). Marginal efficiencies of SAA and methionine utilization derived from the linear-plateau model were 0.43 and 0.65 respectively. Based on linear-plateau and quadratic-plateau models, daily requirements of SAA and methionine for a 50 kg pig were estimated to be 13.0 and 5.9 g and 14.3 and 6.1 g respectively.     

Digestible and metabolizable energy concentrations and amino acid digestibility of dried yeast and soybean meal for growing pigs

Energy values and amino acid (AA) digestibility of dried yeast (DY) and soybean meal (SBM) were determined in 2 experiments with growing pigs. Experiment 1 was conducted to determine the digestible energy (DE) and metabolizable energy (ME) in DY and SBM. Thirty barrows with a mean initial body weight (BW) of 20.6 kg (SD = 1.04) were assigned to 5 dietary treatments in a randomized complete block design with period and BW as blocking factors. A reference diet was prepared with corn, canola meal, and soybean oil as energy-contributing ingredients. Four additional diets were prepared by adding 5% and 10% DY or SBM at the expense of energy-contributing ingredients in the reference diet. The ratio of corn, canola meal, and soybean oil was kept consistent across the experimental diets. Each experimental period consisted of 5-d adaptation and 5-d quantitative collection of feces and urine. Test ingredient-associated DE or ME intake (kcal/d) was regressed against test ingredient intake [kg dry matter (DM)/d] to estimate the DE or ME in test ingredients as the slope of linear regression model. The DE in DY was estimated at 3,933 kcal/kg DM, which was not different from the estimated DE in SBM at 4,020 kcal/kg DM. Similarly, there was no difference between DY and SBM in the estimated ME (3,431 and 3,756 kcal/kg DM, respectively). Experiment 2 was conducted to determine the standardized ileal digestibility (SID) of AA in DY and SBM. Twenty-one barrows with a mean initial BW of 20.0 kg (SD = 1.31) were surgically fitted with T-cannulas at the distal ileum and assigned to 3 dietary treatments in a randomized complete block design with BW as a blocking factor. Two semi-purified diets containing DY or SBM as the sole nitrogen source and one nitrogen-free diet (NFD) were prepared. The NFD was used to estimate the basal ileal endogenous losses of CP and AA. Pigs were fed the 3 diets for 5 d as adaptation, followed by 2 d of feeding with ileal digesta collection. The SID of AA, except Gly and Pro, in DY was less (P < 0.05) than in SBM. The SID of indispensable AA in DY ranged from 64.1% for Thr to 85.2% for Arg, and those in SBM ranged from 83.9% for Thr to 91.8% for Arg. In conclusion, energy values of DY are not different from those of SBM, whereas AA in DY is less digestible than in SBM. The estimated DE and ME as well as the SID of AA in DY and SBM can be used in diet formulation for growing pigs using these ingredients.     

内源性调控对猪采食量的影响

采食量是猪营养的第一制约因素。本文就影响猪采食量调节的因素以及当前内源性调控采食量的一些初步研究作一简要介绍。     

The effect of feed intake on ileal rate of passage and apparent amino acid digestibility determined with or without correction factors in pigs

The apparent ileal digestibilities of amino acids and rate of passage were evaluated in pigs (BW = 78.3 +/- 7.4 kg) fed a semipurified diet. The pigs were fed 1.82, 2.73, or 3.65 kg DMI/d. The highest level of feed intake was considered to be ad libitum feeding. The pigs were fed according to a 3 x 3 Latin square design and were allowed to adapt to each experimental diet for 5 d. This was followed by 1 d of continuous collection of ileal digesta and a 2nd d of continuous collection separated into six 2-h postprandial time blocks. Ytterbium chloride hexahydrate was used to determine rate of passage. The ileal digestibilities of amino acids and rate of passage were unaffected (P > 0.05) by level of feed intake. The use of correction factors to more accurately express amino acid concentrations in the diet and digesta affected (P < 0.05) the apparent ileal digestibility coefficients of some amino acids.     

Apparent digestibility of nitrogen and amino acids in protein feedstuffs by growing pigs

Eight digestion trials were conducted with pigs fitted with ileal T-cannulas to determine the apparent digestibilities of N and amino acids in common protein feedstuffs. Trial-to-trial variation was minimal as determined by the variation in digestibilities for soybean meal, which was evaluated in each trial. Apparent digestibilities varied widely; ileal N digestibility ranged from 88 to 63%, and ileal lysine digestibility ranged from 93 to 40%. In general, ring-dried blood meal, corn gluten meal, Menhaden fish meal, poultry-by-product meal and extruded whole soybeans were the most digestible, followed by canola meal, sunflower meal, peanut meal and meat and bone meal. Cottonseed meal and feather meal were the least digestible. Mean values for each feedstuff agree well with published data. Variation in digestibilities among samples of the same feedstuff was greater for the meat and bone meals. Regression of ileal essential amino acid digestibilities on ileal and fecal N digestibility indicated that amino acid digestibilities can be predicted more precisely from ileal N digestibility than from fecal N digestibility. However, neither ileal nor fecal N digestibility could be used with a high degree of certainty to predict ileal amino acid digestibilities.     

Ileal amino acid digestibility and performance of growing pigs fed wheat-based diets supplemented with xylanase

Two experiments were conducted to determine the effect of supplementation of xylanase to a wheat-based diet on the apparent ileal digestibility (AID) of AA and the performance of growing pigs fed diets limiting in AA. In Exp. 1, eight pigs (average initial BW = 20.5+/-1.2 kg) fitted with a simple T-cannula at the distal ileum, were fed four diets according to a repeated 4 x 4 Latin square design. Diet 1 was a basal diet that contained 97.6% wheat. Diets 2, 3, and 4 were the basal diet supplemented with xylanase at rates of 5,500, 11,000, and 16,500 units of xylanase activity (XU), respectively (as-fed basis). There were linear and quadratic effects (0.062 < P < 0.001) of xylanase supplementation on the AID of CP and most of the AA. The largest increases in AID of CP and AA were obtained when xylanase was supplemented at a rate of 11,000 XU; no further increases were observed with xylanase supplementation at a rate of 16,500 XU. In Exp. 2, 30 pigs (average initial BW 21.4+/-1.8 kg) were randomly allotted to six dietary treatments. Diets 1 to 4 were similar to those used in Exp. 1. Diet 5 was the same as Diet 1, but supplemented with 0.53% lysine, 0.12% threonine, and 0.05% methionine. Diet 6 (positive control diet) was a wheat-soybean meal diet that contained 18.2% CP (as-fed basis). The total contents of lysine, threonine, and methionine were similar for Diets 5 and 6. There was a linear effect of xylanase supplementation on ADG (P = 0.093) and feed:gain ratio (P = 0.089), and a quadratic effect on ADG (P = 0.067) and feed:gain ratio (P = 0.074). But, the greatest response was obtained with the supplementation of 11,000 XU. The supplementation of lysine, threonine, and methionine to Diet 1 increased (P = 0.001) ADG and ADFI and improved (P = 0.01) feed:gain ratio. There was no difference (P = 0.508) in the performance of pigs fed the AA-supplemented or control diet. In conclusion, the supplementation of xylanase to a diet in which wheat provided the sole source of protein and energy improved the AID of AA, ADG, and feed:gain ratio; however, this improvement was very small compared with that obtained with the supplementation of synthetic amino acids.     

内源配体对氨基酸螯合锌吸收的影响

Evans(1975)提出了锌的吸收机制,认为锌必须和胰腺分泌的小分子量蛋白配体(二肽)形成络合物才能被动物吸收。许多研究表明锌不能以离子的形式转运,必须与内源或外源配体形成络合物后,才能被吸收。Seal(1983)、Joerg(1984)、Sandstrom(1985)、Raul(1986)、Scholnerich(1987)等研究表明,组氨酸、色氨酸、脯氨酸、半胱氨酸、天冬氨酸等能促进动物对锌的吸收。关于氨基酸促进锌吸收的机制,研究者多认为,在肠道中,氨基酸和锌可以形成螯合物,使分子内电荷趋于零,易于通过富含阴离子的小肠上皮细胞膜,或通过氨基酸转运系统转运氨基酸螯合锌。本…     

不同处理方法对动物性蛋白质饲料氨基酸消化率的影响

1氨基酸的限制顺序在讨论影响蛋白质品质的因素之前,我们首先要讨论哪一种氨基酸在动物性饲料中最易缺乏。肉骨粉和家禽副产物的氨基酸限制或缺乏的顺序在表