共查询到20条相似文献,搜索用时 31 毫秒
1.
Yoshiro Mano Masanori Muraki Masahiro Fujimori Tadashi Takamizo Bryan Kindiger 《Euphytica》2005,142(1-2):33-42
Adventitious root formation (ARF) at the soil surface is one of the most important adaptations to soil flooding or waterlogging. Quantitative trait loci (QTL) controlling ARF under flooding condition were identified in a 94 F2 individual population by crossing maize (Zea mays L., B64) × teosinte (Z. mays ssp. huehuetenangensis). A base-map was constructed using 66 SSR and 42 AFLP markers, covering 1,378 cM throughout all ten maize chromosomes. The ARF capacity for seedlings was determined by evaluating the degree of root formation at the soil surface following flooding for 2 weeks. ARF showed continuous variation in the F2 population. Interval mapping and composite interval mapping analyses revealed that the QTL for ARF was located on chromosome 8 (bin 8.05). Utilising a selective genotyping strategy with an additional 186 F2 population derived from the same cross combination and 32 AFLP primer combinations, regions on chromosomes 4 (bin 4.07) and 8 (bin 8.03) were found to be associated with ARF. Z. mays ssp. huehuetenangensis contributed all of the QTL detected in this study. Results of the study suggest a potential for transferring waterlogging tolerance to maize from Z. mays ssp. huehuetenangensis. 相似文献
2.
Molecular mapping of a new gene for resistance to frogeye leaf spot of soya bean in 'Peking' 总被引:5,自引:0,他引:5
Amplified fragment length polymorphism (AFLP) and microsatellite (simple sequence repeat, SSR) techniques were used to map the _RGSpeking gene, which is resistant to most isolates of Cercospora sojina in the soya bean cultivar ‘Peking’. The mapping was conducted using a defined F2 population derived from the cross of ‘Peking’(resistant) בLee’(susceptible). Of 64 EcoRI and MseI primer combinations, 30 produced polymorphisms between the two parents. The F2 population, consisting of 116 individuals, was screened with the 30 AFLP primer pairs and three mapped SSR markers to detect markers possibly linked to RcsPeking. One AFLP marker amplified by primer pair E‐AAC/M‐CTA and one SSR marker Satt244 were identified to be linked to ResPeking. The gene was located within a 2.1‐cM interval between markers AACCTA178 and Satt244, 1.1 cM from Satt244 and 1.0 cM from AACCTA178. Since the SSR markers Satt244 and Satt431 have been mapped to molecular linkage group (LG) J of soya bean, the ResPeking resistance gene was putatively located on the LG J. This will provide soya bean breeders an opportunity to use these markers for marker‐assisted selection for frogeye leaf spot resistance in soya bean. 相似文献
3.
Teosinte comprises different Zea species (Zea mays, Zea diploperennis, Zea perennis, Zea luxurians) that can be crossed with cultivated maize (Z. mays ssp. mays). Nine microsatellites from maize were applied to different teosinte species in order to evaluate their usefulness in markerbased exploitation of these genetic resources. The same microsatellites were tested with rye, barley, and sorghum as potential molecular markers for these species. Almost all microsatellite × teosinte combinations yielded polymerase chain reaction (PCR) fragments in the range of cultivated maize. Using an F2 population of a cross between maize inbred A188 and an individual of Zea mays ssp. mexicana, amplification products for maize and teosinte originated from the same genomic location for each of nine microsatellites investigated. PCR fragments of reduced intensity were generally obtained by applying maize microsatellites to rye, barley and sorghum. Polymorphisms among accessions within teosinte (sub)species occurred frequently. In contrast, no polymorphisms were obtained within rye, barley, and sorghum. Hence, application of maize microsatellites to teosinte for fingerprinting or marker-assisted introgression of genomic regions from teosinte into cultivated maize appears promising. 相似文献
4.
玉米抗丝黑穗病QTL分析 总被引:13,自引:1,他引:12
以Mo17(抗)×黄早四(感)的F2分离群体(191个单株)为作图群体,构建了含有84个SSR位点和48个AFLP位点的遗传连锁图谱,全长1 542.9 cM,平均图距11.7 cM。在吉林省公主岭和黑龙江省哈尔滨2个地点通过人工接种方法对184个相应的F3家系(缺失7个)进行抗病鉴定。采用复合区间作图法对抗丝黑穗病数量性状位点(QTL)进行定位及遗传效应分析。在吉林公主岭地区检测到5个QTL,分别位于第1、2、3、8、9染色体上,解释的表型方差为10.0%~16.3%。在黑龙江哈尔滨地区也检测到5个QTL,分别位于第1、2、3、4、7染色体上,解释的表型方差为4.6%~13.4%。比较分析发现,两地一致在第2、3染色体上各检测到1个QTL,其中第2染色体上的表现为超显性效应,第3染色体上的表现为加性效应。研究结果为玉米抗丝黑穗病种质改良提供了重要信息。 相似文献
5.
Amplified fragment length polymorphism- and simple sequence repeat-based molecular tagging and mapping of greenbug resistance gene Gb3 in wheat 总被引:3,自引:0,他引:3
The greenbug, Schizaphis graminum (Rondani), is the most economically damaging aphid pest of wheat in the southern Great Plains of the USA. In this study, the single, dominant greenbug resistance gene, Gb3, was molecularly tagged and genetically mapped using amplified fragment length polymorphism (AFLP) and simple sequence repeat(SSR) markers. Three AFLP loci were associated with the Gb3 locus in linkage analysis with 75 F2:3 families from the cross between two near‐isogenic lines (NILs) for Gb3,‘TXGBE273’ and ‘TXGBE281′. Two of these loci, XMgcc Pagg and Xmagg Patg cosegregate with Gb3 in the population analysed. Further analysis indicated that XMgcc Pagg and Xmagg Patg are specific for the Gb3 locus in diverse genetic backgrounds. Two SSR markers, Xgwm111 and Xgwm428 previously mapped in wheat chromosome 7D, were shown to be linked with Gb3, 22.5 cM and 33.1 cM from Gb3, respectively, in an F2 population of ‘Largo’בTAM 107’, suggesting that Gb3 is located in the long arm of chromosome 7D. The two AFLP markers cosegregating with Gb3 are valuable tools in developing molecular markers for marker‐assisted selection of greenbug resistance in wheat breeding. 相似文献
6.
R. A. Pickering 《Plant Breeding》1987,99(3):177-180
Investigations were carried out to assess the suitability of the intergeneric cross Hordeum vulgare×Psathyrostachys fragilis for haploid barley production. H. vulgare cvs. ‘Emir’ and ‘Vada’ were each pollinated with P. fragilis P.I. 343192 and plants regenerated from embryos cultured on a modified B5 medium. Seed sets on ‘Vada’ were significantly lower than on ‘Emir’, and all the planes from ‘Vada’×P. fragile remained hybrid. Several of these flowered but there was little pairing between the parental chromosomes. Most of the plants from ‘Emir’×P. fragilis died, as seedlings but 3 plants developed into haploid barley. Because of the practical limitations of pollen availability from P. fragilis and the inconsistencies in haploid plant formation, it is unlikely that the cross will prove as valuable as that between H. vulgare×H. bulbosum for a doubled haploid barley programme. 相似文献
7.
B. C. Y. Collard R. A. Grams W. D. Bovill C. D. Percy R. Jolley A. Lehmensiek G. Wildermuth M. W. Sutherland 《Plant Breeding》2005,124(6):532-537
Crown rot, caused by Fusarium pseudograminearum, is an important disease of wheat in Australia and elsewhere. In order to identify molecular markers associated with partial seedling resistance to this disease, bulked segregant analysis and quantitative trait loci (QTL) mapping approaches were undertaken using a population of 145 doubled haploid lines constructed from ‘2‐49’ (partially resistant) × ‘Janz’ (susceptible) parents. Phenotypic data indicated that the trait is quantitatively inherited. The largest QTLs were located on chromosomes 1D and 1A, and explained 21% and 9% of the phenotypic variance, respectively. Using the best markers associated with five QTLs identified by composite interval mapping, the combined effect of the QTLs explained 40.6% of the phenotypic variance. All resistance alleles were inherited from ‘2‐49’ with the exception of a QTL on 2B, which was inherited from ‘Janz’. A minor QTL on 4B was loosely linked (19.8 cM) to the Rht1 locus in repulsion. None of the QTLs identified in this study were located in the same region as resistance QTLs identified in other populations segregating for Fusarium head blight, caused by Fusarium graminearum. 相似文献
8.
In the presented study, the existing AFLP and SSR maps of barley were used to find chromosomal position of four genes controlling
different stages of root hair development. Four barley mutants were used in the analysis: the root hairless mutant rhl1.b, mutant rhp1.b with root hair development blocked at the initial bulge formation, mutant rhi1.a with irregular pattern of sparsely located root hairs and mutant rhs1.a with very short root hairs. Each mutant was crossed with parents of ‘Steptoe’/‘Morex’ mapping population and F2 progenies of crosses: mutant × ‘Steptoe’ and mutant × ‘Morex’ were analyzed for segregation of root hair phenotype and polymorphic
AFLP and SSR markers. It was possible to map all the analyzed genes on barley chromosomes: rhl1 gene on the short arm of chromosome 7H, rhp1 gene on chromosome 1H, rhs1 locus in the pericentromeric region of chromosome 5H and rhi1 gene on the long arm of chromosome 6H. Subsequently, the Bulk Segregant Analysis and AFLP technique were used for saturation
of the identified regions with new markers. The joint maps were constructed using as common points the SSR markers located
in the target regions. Linkage maps of the regions around the four genes involved in the root hair formation in barley were
composed of 8–11 markers and spanned over 16.1–49.0 cM. The distances between localized genes and the closest markers ranged
from 1.0 to 3.8 cM. The identified chromosomal locations of genes can be used for their fine mapping and future map-based
cloning. 相似文献
9.
Harpinder Singh Randhawa Zlatko Popovic Jim Menzies Ron Knox Stephen Fox 《Euphytica》2009,169(2):151-157
This study was conducted to determine the genetic control of resistance to loose smut caused by Ustilago tritici race T33 in two durum recombinant inbred line populations (DT662 × D93213 and Sceptre × P9162-BJ08*B) and to identify molecular
markers linked to the resistance. Resistance in both populations was controlled by single genes. Two SSR markers were linked
with loose smut resistance in the Sceptre × P9162-BJ08*B population. In DT662 × D93213, two AFLP, two wheat SSRs and one SCAR
markers were linked to resistance. The SCAR marker, 3.2 cM distal to the smut resistance locus (Utd1) on chromosome 5BS, accounted for up to 64% of the variability in disease reaction; the other markers were proximal to Utd1 at genetic distances ranging from 5.9 to 35.9 cM. SSR markers Xgwm234 and Xgwm443 segregated in both crosses suggesting a common resistance gene. The SCAR and SSR markers can be used effectively for marker
assisted selection to incorporate loose smut resistance into durum cultivars. 相似文献
10.
A. M. M. Barbosa I. O. Geraldi L. L. Benchimol A. A. F. Garcia C. L. Souza Jr. A. P. Souza 《Euphytica》2003,130(1):87-99
Two sets of tropical maize inbred lines, one derived from the BR-105 population and another derived from the BR-106 population,
were assayed for Amplified Fragment Length Polymorphism's (AFLP) and for Simple Sequence Repeat (SSR), in order to investigate
genetic distances among lines and their relationship to heterotic group assignment and single cross yield performance. Genetic
distances were on average greater for interpopulation than intrapopulation crosses for both AFLP and SSR. Cluster analysis
was in agreement with the original assignment for heterotic groups. Inbred line 16, derived from BR-106, was assigned to the
BR-105 set, in agreement with single cross yield performance from intra- and interpopulation crosses. However, the same pattern
was not observed for SSR where another two lines from BR-106 were also assigned to the BR-105 set. Correlation coefficients
of genetic distances (GD) with F1 grain yield and heterosis were high for BR-106 ×BR-106 crosses
(0.91** and 0.82** for AFLP and SSR, respectively), moderate for BR-105 × BR-105 crosses (0.52* for AFLP and SSR) and low for BR-105 × BR-106 crosses (0.29 and 0.16 for AFLP and SSR, respectively). The lower correlation
at interpopulation level was due to the smaller range of GD caused probably by a previous selection for combining ability.
General results showed that the AFLP molecular marker is efficient in assigning maize lines to heterotic groups and that AFLP-based
GD is suitable for predicting the maize single cross performance for intrapopulation crosses of broad-based populations. The
efficiency of SSR in assigning lines to heterotic groups and for predicting single cross performance was smaller than AFLP.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
11.
Lagerstroemia (crape myrtle) are famous ornamental plants with large pyramidal racemes, long flower duration and diverse colours. Genetic maps provide an important genomic resource of basic and applied significance. A genetic linkage map was developed by genotyping 192 F1 progeny from a cross between L. caudata (female) and L. indica (‘Xiang Xue Yun’) (male) with a combination of amplification fragment length polymorphisms (AFLP) and simple sequence repeats (SSR) markers in a double pseudo‐testcross mapping strategy. A total of 330 polymorphic loci consisting of 284 AFLPs and 46 SSRs showing Mendelian segregation were generated from 383 AFLP primer combinations and 150 SSR primers. The data were analysed using JoinMap 4.0 (evaluation version) to construct the linkage map. The map consisted of 20 linkage groups of 173 loci (160 AFLPs and 13 SSRs) covering 1162.1 cM with a mean distance of 10.69 cM between adjacent markers. The 20 linkage groups contained 2–49 loci and ranged in length from 7.38 to 163.57 cM. This map will serve as a framework for mapping QTLs and provide reference information for future molecular breeding work. 相似文献
12.
Gerald O. Myers Baogong Jiang Muhanad W. Akash Ashok Badigannavar Sukumar Saha 《Euphytica》2009,165(2):391-399
In this research, we used two sets of cotton aneuploid (G. hirsutum × G. tomentosum and G. hirsutum × G. barbadense) plants to locate AFLP markers to chromosomes using deletion analysis method. Thirty-eight primer combinations were used
to generate 608 polymorphic AFLP markers. A total of 98 AFLP markers were assigned to 22 different cotton chromosomes or chromosome
arms. Of those assigned markers, 63.3% were assigned to A genome and 36.7% were assigned to D genome. A low rate (14.3%) of
common markers were found between those assigned AFLP markers with the AFLP markers from an intraspecific cross population
developed previous in our lab. Based on the 16 common markers, we were able to associate the 13 linkage groups previously
identified in our lab to eight chromosomes. Further research will be carried out by using SSR markers with known location
to associate unassigned linkage groups to chromosomes. 相似文献
13.
Genetic study on important traits of tea is difficult because of its self-incompatibility in nature. Moreover, development of a new variety usually needs more than 20 years, since it takes many years from seedling to matured plants for trait investigation. Genetic map is an essential tool for genetic study and breeding. In this study, we have developed an integrated genetic map of tea (Camellia sinensis) using a segregating F1 population derived from a cross between two commercial cultivars (‘TTES 19’ and ‘TTES 8’). A total of 574 polymorphic markers (including SSR, CAPS, STS, AFLP, ISSR and RAPD), 69 markers with highly significant levels of segregation distortion (P < 0.001) (12.0 %) were excluded from further analyses. Of the 505 mapped markers, there were 265 paternal markers (52.5 %), 163 maternal markers (32.3 %), 65 doubly heterozygous dominant markers (12.9 %), and 12 co-dominant markers (2.4 %). The co-dominant markers and doubly heterozygous dominant markers were used as bridge loci for the integration of the paternal and maternal maps. The integrated map comprised 367 linked markers, including 36 SSR, 3 CAPS, 1 STS, 250 AFLP, 13 ISSR and 64 RAPD that were assigned to 18 linkage groups. The linkage groups represented a total map length of 4482.9 cM with a map density of 12.2 cM. This genetic map has the highest genetic coverage so far, which could be applied to comparative mapping, QTL mapping and marker assisted selection in the future. 相似文献
14.
玉米抗纹枯病QTL定位 总被引:10,自引:1,他引:9
以玉米自交系R15(抗)×掖478(感)的229个F2单株为作图群体,构建了包含146个SSR标记位点的遗传连锁图谱,全长1 666 cM,平均图距11.4 cM。通过麦粒嵌入法对F2:4群体进行人工接种纹枯病菌,并以相对病斑高为病级划分标准鉴定了玉米纹枯病的抗性。用复合区间作图法分析抗病QTL及遗传效应,共检测到9个抗性QTL,分布于第1、2、3、4、5、6和10条染色体上,单个QTL可解释表型方差的3.72%~7.19%,其中有2个QTL位于染色体6.01抗病基因簇附近。 相似文献
15.
Manli Li Nana Yuyama Mariko Hirata Jianguo Han Yunwen Wang Hongwei Cai 《Euphytica》2009,170(3):327-338
A consensus genetic linkage map with 447 SSR markers was constructed for zoysiagrass (Zoysia japonica Steud.), using 86 F1 individuals from the cross ‘Muroran 2’ × ‘Tawarayama Kita 1’. The consensus map identified 22 linkage groups and had a total
length of 2,009.9 cM, with an average map density of 4.8 cM. When compared with a previous AFLP-SSR linkage map, the SSR markers
from each linkage group mapped to similar positions in both maps. Eight pairs of linkage groups from the AFLP-SSR map were
joined into eight new groups in the current map. This zoysiagrass consensus map contained 35 SSR markers exhibiting high homology
with rice genomic sequences from known chromosomal locations. This allowed synteny to be identified between Zoysiagrass linkage
groups 2, 3, 9, 19 and rice chromosomes 3, 12, 2, 7 respectively. These results provide important comparative genomics information
and the new map is now available for quantitative trait locus analysis, marker-assisted selection and breeding for important
traits in zoysiagrass. 相似文献
16.
QTL analysis of resistance to Fusarium head blight in wheat using a 'Frontana'-derived population 总被引:6,自引:1,他引:6
M. Mardi L. Pazouki H. Delavar M. B. Kazemi B. Ghareyazie B. Steiner R. Nolz M. Lemmens H. Buerstmayr 《Plant Breeding》2006,125(4):313-317
Fusarium head blight (FHB or head scab) has become a major limiting factor for sustainable wheat (Triticum aestivum L.) production around the world. For quantitative trait loci (QTL) analysis of resistance to FHB, F3 plants and F3 : 5 lines, derived from a ‘Frontana’ (moderately resistant)/‘Seri82’ (susceptible) cross, were spray‐inoculated in 2001 and 2002, respectively. Artificial inoculations were carried out under field conditions. Of 273 SSR and AFLP markers, 250 could be mapped and they yielded 42 linkage groups, covering a genetic distance of 1931 cM. QTL analysis was based on the constructed linkage map and area under the disease progress curve (AUDPC). The analyses revealed three consistent QTLs associated with FHB resistance on chromosomes 1BL, 3AL and 7AS explaining 7.9%, 7.7% and 7.6% of the phenotypic variation, respectively, above 2 years. The results confirmed the previously described resistance QTL of ‘Frontana’ on chromosome 3AL. A combination of ‘Frontana’ resistance with ‘Sumai‐3’ resistance may lead to lines with augmented resistance expression. 相似文献
17.
To further utilize the valuable germplasm Orychophragmus violaceus for Brassica genetics and breeding, a B. napus × O. violaceus cross was repeated with embryo rescue. All F1 plants except one B. napus haploid were mixoploids (2n = 17–39 in ovaries) with 2n = 31, 37, 38 and 39 as the maximal chromosome numbers in individuals, but the higher numbers mostly appeared in pollen mother cells (PMCs) with a preponderance of 2n = 30, 37 and 38. Only one chromosome and one chromosome segment of O. violaceus were detected at a low frequency in some ovary cells and PMCs with 2n = 37, 38 and 39 as determined by genomic in situ hybridization analysis. The fatty acid profiles of seeds from the majority of the F1 and F2 plants were similar to those of female B. napus cv. ‘Oro’, but some were obviously different in the percentages of oleic, linoleic and erucic acids, and some F2 plants (2n = 38) with good seed set had high percentages of oleic (>70.0%) or linoleic (to 38.3%) acids and low erucic acid (<1%). Subsequently, many kinds of B. napus aneuploids (2n = 28, 30, 34, 36, 37, 39 and 42), without O. violaceus chromosomes, were derived from F2 progeny and microspores of partial F1 plants. Finally, the cytological mechanisms behind the variations in chromosome numbers were discussed together with the implications of these aneuploids for Brassica genome research and of the plants with altered fatty acid profiles for improving the oil quality of B. napus. 相似文献
18.
Chromosomal location of powdery mildew resistance gene Pm16 in wheat using SSR marker analysis 总被引:10,自引:0,他引:10
X. M. Chen Y. H. Luo X. C. Xia L. Q. Xia X. Chen Z. L. Ren Z. H. He J. Z. Jia 《Plant Breeding》2005,124(3):225-228
The use of resistant cultivars is a most economical way to control powdery mildew (Blumeria graminis f.sp. tritici) in wheat (Triticum aestivum L.). Identification of molecular markers closely linked to resistance genes can greatly increase the efficiency of pyramiding resistance genes in wheat cultivars. The objective of this study was to identify molecular markers closely linked lo the powdery mildew resistance gene Pm16. An F2 population with 156 progeny was produced from the cross‘Chancellor’(susceptible) ב70281’ (resistant), A total of 45 SSR markers on chromosomes 4A and 5B of wheat and 15 SSRs on chromosome 3 of rice was used lo lest the parents, as well as the resistant and susceptible bulks: the resulting polymorphic markers were used to genotype the F2 progeny. Results indicated that the SSR marker Xgwm159, located on the short arm of chromosome 5B, is closely linked to Pm16 (genetic distance: 5.3 CM). The cytogenetical data presented in an original report, in combination with this molecular analysis, suggests that Pm16 may he located on a translocated 4A.5BS chromosome. 相似文献
19.
W. D. Bovill W. Ma K. Ritter B. C. Y. Collard M. Davis G. B. Wildermuth M. W. Sutherland 《Plant Breeding》2006,125(6):538-543
Crown rot (causal agent Fusarium pseudograminearum) is a fungal disease of major significance to wheat cultivation in Australia. A doubled haploid wheat population was produced from a cross between line ‘W21MMT70’, which displays partial seedling and adult plant (field) resistance to crown rot, and ‘Mendos’, which is moderately susceptible in seedling tests but partially resistant in field trials. Bulked segregant analysis (BSA) based on seedling trial data did not reveal markers for crown rot resistance. A framework map was produced consisting of 128 microsatellite markers, four phenotypic markers, and one sequence tagged site marker. To this map 331 previously screened AFLP markers were then added. Three quantitative trait loci (QTL) were identified with composite interval mapping across all of the three seedling trials conducted. These QTL are located on chromosomes 2B, 2D and 5D. The 2D and 5D QTL are inherited from the line ‘W21MMT70’, whereas the 2B QTL is inherited from ‘Mendos’. These loci are different from those associated with crown rot resistance in other wheat populations that have been examined, and may represent an opportunity for pyramiding QTL to provide more durable resistance to crown rot. 相似文献
20.
Cashew (Anacardium occidentale) is a widespread tropical tree crop that is grown primarily for its nuts and has a global production of over 2 million Mt.
In spite of its economic importance to many countries, however, no linkage map containing STS anchor sites has yet been produced
for this species. This is largely attributable to a prolonged juvenile phase of the tree (limiting mapping to F1 progenies) and difficulty in effecting sufficient hand-pollinations to create mapping populations of effective size. Here,
we produce an F1 mapping population of 85 individuals from a cross between CP 1001 (dwarf commercial clone) and CP 96 (giant genotype), and
use it to generate two linkage genetic maps comprising of 205 genetic markers (194 AFLP and 11 SSR markers). The female map
(CP 1001) contains 122 markers over 19 linkage groups and the male map (CP 96) comprises 120 markers assembled over 23 linkage
groups. The total map distance of the female map is 1050.7 cM representing around 68% genome coverage, whereas the male map
spans 944.7 cM (64% coverage). The average map distance between markers is 8.6 cM in the female map and 7.9 cM in the male
map. Homology between the two maps was established between 13 linkage groups of the female map and 14 of the male map using
46 bridging markers that include 11 SSR markers. These maps represent a platform from which to identify loci controlling economically
important traits in this crop. 相似文献