蔗糖和B_(12)对昆明地区油橄榄扦插生根的影响

在昆明市海口林场种植的油橄榄树干较易萌芽,芽条生长快,但是由于管理粗放,插穗营养不良,扦插难生根。因此,从油橄榄喜光、耐高温且插穗营养不良的角度出发,在高温高湿条件下,针对蔗糖、B12及插床设计对油橄榄扦插生根的影响进行试验研究。结果表明,插穗经采用100 mg/l IBA+5%蔗糖+10 ml B12浸泡2 h处理生根率可达69.3%,平均不定根数可达10.9条,平均不定根长4.2 cm,根系效果指数为0.60。插床空间越小,生根效果越好。     

小窄径茧蜂林间套笼接蜂试验

小窄径茧蜂林间套笼接蜂试验结果表明,影响小窄径茧蜂寿命和林间寄生活动的主要因素是接蜂时间和套笼内温度。７ 月下旬接蜂,寄生率为８．５２％ ～８．９０％ ,７ 月上旬接蜂,寄生率只有１．４０％ ～２．１６％ 。笼内温度超过３０～３４℃,小窄径茧蜂存活时间只有２～４天,少数可存活６～７ 天。在抚顺地区７ 月下旬至８月初是小窄径茧蜂寄生活动的最佳时间。试验表明,小窄径茧蜂可以在我国兴安落叶松鞘蛾上寄生并安全越冬。     

吉林市森林固碳释氧生态效益评价

以吉林市现有森林资源面积和各林龄组面积、蓄积为基础数据,按国家林业行业标准规定的方法,测算森林年净生产力、固碳量与释氧量和货币价值量.结果表明:森林净生产力为1 822 783.3 t·a-1,固碳量为2 835 277.2 t·a-1,释氧量为2 168 711.2 t·a-1;固碳价值为34.02亿元·a-1,释氧...     

沈阳市5种绿化树种固碳释氧与降温增湿效应研究

为给沈阳市园林绿地树种的选择与配置提供科学依据,该研究选择沈阳市园林绿化的5种主要乔木作为试验材料,利用LI-6400便携式光合仪测定了光合生理指标,并对其固碳释氧和降温增湿效应进行了初步量化比较。结果表明,春季单位叶面积日固碳释氧能力为银中杨大叶朴稠李银杏紫椴;降温增湿能力为银中杨大叶朴紫椴银杏稠李。银中杨改善环境碳氧平衡、降温增湿作用均优于其他4种乔木,调节小气候的能力较强。     

Individual and combined effects of rhynchophylline and ketamine on proliferation,NMDAR1 and GluA2/3 protein expression in PC12 cells

Rhynchophylline is an active component of the Uncaria species, which is a member of the Rubiaceae family. Our studies show that the downregulation of N-methyl-d-aspartate (NMDA) receptor subunit GluN2B expression in the nucleus accumbens, amygdala, medial prefrontal cortex, and hippocampal CA1 area by rhynchophylline is beneficial for the treatment of psychological dependence on amphetamines. The individual and combined effects of rhynchophylline and ketamine on proliferation and GluN1 and GluA2/3 protein expression in PC12 cells were investigated. PC12 cells were differentiated into neuron-like cells by treatment with nerve growth factor (50 ng/mL). After treatment for 48 h, differentiated PC12 cell proliferation and GluN1 and GluA2/3 protein expression were analyzed. The viability of PC12 cells was reduced by ketamine at doses of 0.50, 1.00, 1.50, and 2.00 mmol/L, with the viability of cells treated with 1.50 and 2.00 mmol/L of ketamine significantly lower than that of the control cells. However, PC12 cells treated with rhynchophylline showed no toxicity at doses of 0.25, 0.50, 0.75, or 1.00 mmol/L. While GluA2/3 protein expression was upregulated by ketamine, it was not influenced by rhynchophylline. GluN1 protein expression was downregulated by rhynchophylline (1 mmol/L), while treatment with ketamine, either alone or with rhynchophylline, had no effect. These findings demonstrate that rhynchophylline suppresses GluA2/3 expression in ketamine-induced PC12 cells and downregulates GluN1 expression. Ketamine's lack of effect on GluN1 expression offers a partial explanation for ketamine addiction and the anti-addictive properties of rhynchophylline.     

Effects of Pinus massoniana bark extract on the adhesion and migration capabilities of HeLa cells

Pinus massoniana Lamb is a Chinese red pine species used in traditional medicine for the treatment of a variety of human health disorders. Recent studies have shown that P. massoniana bark extract (PMBE) has an anti-proliferation effect on cancer cells. However, it is not clear if PMBE affects cancer cell migration and/or invasion. We tested the effect of PMBE, which has B-type procyanidin as its main constituent, on the adhesion and migration capabilities of HeLa cells, a human cervical cancer cell line, cultured in vitro. Our results showed that PMBE has no significant effect on the adhesion capability of HeLa cells, but strongly inhibits their migration. This finding suggests that PMBE could be a potential therapeutic agent for metastatic cancer.     

刚果12号桉W5无性系与尾叶桉U6无性系胶合板材立木出材量材积推导研究

利用雷州林业局刚果12号桉W 5无性系林分中胸径8~18 cm径阶,树高11~23 m的样木1 207株和尾叶桉U6无性系林分中胸径8~18 cm径阶,树高10~21 m的样木784株,按材种规格标准造材实测材积,且分别建立刚果12号桉W 5无性系和尾叶桉U6无性系胶合板材立木出材量二元回归方程,经检验精度可靠,可供参考使用。     

Effects of Swertia japonica extract and its main compound swertiamarin on gastric emptying and gastrointestinal motility in mice

The Swertia japonica is used clinically as a remedy for gastrointestinal symptoms in Japan. We examined the effects of a S. japonica and swertiamarin on gastric emptying and gastrointestinal motility in atropine-, dopamine-, and 5-hydroxytryptamine (5-HT)-treated mice. All three preparations inhibited reductions in gastric emptying and gastrointestinal motility induced by dopamine (1 mg/kg, intraperitoneal injection, ip). Neither the powder, swertiamarin, nor itopride had any effect on the reductions in gastric emptying and gastrointestinal motility caused by 5-HT (4 mg/kg, ip). These findings suggest that the powder and swertiamarin stimulate gastric emptying and gastrointestinal motility by inhibiting the dopamine D₂ receptor.     

The effect of berberine in vitro on tight junctions in human Caco-2 intestinal epithelial cells

Berberine is one of the main alkaloids of Rhizoma coptidis which has been used for patients with gastrointestinal disorders. The major aim of this study was to investigate the effect of berberine on tight junction. Caco-2 cells were treated with various concentration of berberine. We observed the integrity of tight junction by measuring the transepithelial electrical resistance (TEER), and also studied the effect of berberine on morphology of tight junction and tight junction protein. These findings showed the first time that berberine could reduce epithelial gut permeability, and might help explain the possible mechanisms of anti-diarrhea activity of berberine.     

雷州刚果12号桉W5无性系林分蓄积量与胸径树高密度及其之间关系的探讨

采用雷州林业局2002年森林二类调查的刚果12号桉W5无性系林分1071块样地中279块样地材料，进行蓄积量与胸径、树高、密度及其之间关系的探讨分析。分析结果表明：胸径是影响林分蓄积量的主要因素，其次是树高与密度；树高与胸径关系密切，呈正相关，但两者的生长随着造林密度的加大而显著下降。因此，进行W5无性系林分密度试验，提出合理的造林密度，是提高其单位面积蓄积量的关键。     

模拟氮沉降对华西雨屏区巨桉林凋落叶分解的影响

2008-01—2010-01,对华西雨屏区巨桉人工林进行氮沉降模拟试验,氮沉降水平分别为对照(CK,0gN.m-2a-1)、低氮(5gN.m-2a-1)、中氮(15gN.hm-2a-1)和高氮(30gN.m-2a-1),把年施氮量分12等份,每月下旬对各处理施氮(NH4NO3),探讨氮沉降持续增加对巨桉凋落叶分解和养分释放过程的影响,及巨桉凋落叶分解过程中是否存在限制值。结果表明:巨桉凋落叶在分解初期存在一个质量快速损失的淋溶期,而分解后期(14个月以后)质量损失极其缓慢,残留凋落物处于较稳定状态;氮沉降显著抑制了巨桉凋落叶后期分解,并且低氮处理抑制作用最强,但氮沉降对凋落物养分释放过程无明显影响;自然分解状态下,巨桉凋落叶分解限制值大约为90%(CK),而氮沉降使得这一限制值降低,并且低氮(限制值大约为72%)与对照之间差异达到显著水平。     

青钱柳叶提取物对Caco-2细胞二糖酶活性的抑制作用

采用Caco-2细胞模型,分析了青钱柳Cyclocarya paliurus叶水提物、醇提后的水提物、95%醇提物、乙酸乙酯萃取物和正丁醇萃取物5种不同提取物对Caco-2细胞活力的影响及对Caco-2细胞上麦芽糖酶、蔗糖酶、乳糖酶3种二糖酶活性的抑制作用。结果表明,5种不同提取物中除95%醇提物对Caco-2细胞活力产生一定影响外,其余4种提取物对Caco-2细胞基本无毒性,在200 mg·L^-1浓度提取物范围内培养24～72 h后细胞仍能保持较高活力;对3种二糖酶活性抑制作用效果最好的为醇提后的水提物,尤其是对蔗糖酶和乳糖酶的抑制作用更为明显,最高的抑制率分别达到76.35%和60.13%,其次为乙酸乙酯萃取物,对乳糖酶的抑制率最高达54.41%。从青钱柳叶不同方法提取物的制备流程说明多糖类或中等极性的黄酮甙类有可能是青钱柳叶中抑制二糖酶活性的主要组成成分。     

Effect of a standardized extract of red orange juice on proliferation of human prostate cells in vitro

A standardized extract of red orange juice (ROE) was shown to inhibit proliferation of fibroblast and epithelial prostate cells. These data suggest that the antiproliferative properties of ROE cannot be ascribed to cytotoxic effect and highlight its potential usefulness in the management of benign prostatic hyperplasia.     

Inhibitory components from the buds of clove (Syzygium aromaticum) on melanin formation in B16 melanoma cells

In the course to find a new whitening agent, we evaluated the methanol extract from bud of clove (Syzygium aromaticum) on melanin formation in B16 melanoma cells. Eugenol and eugenol acetate were isolated as the active compounds and showed melanin inhibition of 60% and 40% in B16 melanoma cell with less cytotoxicity at the concentration of 100 and 200 μg/mL, respectively. Furthermore, an essential oil prepared from the bud of clove, which contain eugenol and eugenol acetate as dominant components, showed melanin inhibition of 50% and 80% in B16 melanoma cells at the concentration of 100 and 200 μg/mL, respectively.     

Effects of the plant alkaloid thaliblastine on non-cross-resistant and sensitive human leukemia cells in relation with reversal of acquired anthracycline resistance

Thaliblastine exhibits dose dependent cytotoxic effect on HL-60, HL-60/DOX, RHE and HD-MY-2 leukemia cells. Additionally, typical for apoptosis oligonucleosomal DNA fragmentation could be detected in leukemia cells treated with thaliblastine. Moreover, an MDR-phenotype reversing effect of thaliblastine was also identified.