水牛梭形住肉孢子虫包囊纯化抗原的SDS—PAGE分析

应用ＳＤＳ－ＰＡＧＥ分析了水牛梭形住肉孢子虫包囊纯化抗原的蛋白质组分。采用垂直板型电泳，连续凝胶系统法，以考马斯亮兰Ｒ－２５０染色进行ＳＤＳ－ＰＡＧＥ分析的结果表明，水牛梭形住肉孢子虫包囊纯化抗原至少由１０种蛋白质组成，分子量范围为１７．５ＫＤ～１３５ＫＤ。其中主要蛋白质组分有５种，分子量分别为１２５ＫＤ、９８ＫＤ、９０ＫＤ、６９ＫＤ及３５ＫＤ。     

牦牛肝片吸虫分泌排泄抗原的生化特性和免疫印迹分析

肝片吸虫的分泌排泄抗原（ＥＳ抗原）在诊断及诱导机体产生抗体方面具有重要作用。本文对寄生于牦牛的肝片吸虫ＥＳ抗原的蛋白质组成及其生化特性进行了分析。ＳＤＳ聚丙烯酰胺凝胶电泳显示ＥＳ抗原共有９条带，主要由１２－２６ＫＤ的小分子量蛋白质组成；糖蛋白及脂蛋白染色后发现ＥＳ抗原中糖蛋白极少，而含有较多的脂蛋白；等电聚焦电泳结果显示ＥＳ抗原有２２条带，几乎全部是酸性蛋白，ｐＩ主要集中在４．２５～５．２５范围内；双向电泳共检出３０个多肽，主要分布在ｐＩ４．５～７．０之间；免疫印迹分析结果表明：ＥＳ抗原中分子量为１６．２ＫＤ～１８．６ＫＤ的蛋白质是主要的抗原成分，其中１７．２ＫＤ多肽具有最强的免疫原性。     

用几种电泳方法分析牦牛肝片吸虫不同部位抗原

应用ＳＤＳ－ＰＡＧＥ、等电聚焦电泳（ＩＥＦ）及双向电泳三种电泳方法分析牦牛肝片吸虫成虫四种抗原（头抗原－ＨＡ、体抗原－ＢＡ、体表抗原ＳＡ、分泌排泄抗原－ＥＳ）。ＳＤＳ－ＰＡＧＥ结果表明，ＢＡ、ＨＡ、ＳＡ分子量在１２～１００ｋＤ之间，ＥＳ在１２．３～２６ｋＤ之间，蛋白质染色ＢＡ、ＨＡ、ＳＡ、ＥＳ分别显示２５、２４、１８、９条带；ＩＥＦ分析肝片吸虫分别得３４、３３、２８、２２条带，主要由酸性蛋白质组成，主要谱带在ｐＩ４．２０～６．５５内；双向电泳分析ＢＡ、ＥＳ多肽斑点为６９、３０个     

旋毛虫肌幼虫可溶性抗原、排泄分泌抗原的电泳分析

本文报道了应用十二烷基硫酸钠一聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ），聚丙烯酰胺凝胶等电聚焦（ＩＥＦ）电泳及二维电泳（ＩＥＦ／ＳＤＳ－ＰＡＧＥ）对旋毛虫肌动虫排泄分泌（ＥＳ）抗原和肌幼虫可溶性抗原的分析结果。肌幼虫ＥＳ抗原经ＳＤＳ－ＰＡＧＥ后用考马斯亮蓝染色蛋白质，结果显示１６条蛋白带，分子量范围２１～８０ＫＤ，其中主带９条。ＩＥＦ电泳后分别用ＰＡＳ染多糖、考马斯亮蓝Ｒ－２５０染蛋白质、Ｎｉｌｅ＇ｓ蓝染脂、醋酸ａ－萘酯／坚固蓝染酪酶同工酶，结果肌幼虫ＥＳ抗原分别显示１６，２６、７及０条带；肌幼虫可溶性抗原分别显示２１、３８、４及１１条带。二维电泳后用考马斯亮蓝Ｇ－２５０染色多肤斑点，结果ＥＳ抗原显示多肽斑点６１个；肌幼虫可溶抗原显示１２２个多肽斑点。     

家兔胚源性特异蛋白－1的分泌特征及生物活性

经ＳｅｐｈａｄｅｘＧ７５和ＰＡＧＥ分离，以双波长薄层自动扫描仪扫描测定了家兔胚源性特异蛋白－１（ｒＥＳＰ－１）的分泌特性；离体组织培养分析了ｒＥＳＰ－１对子宫蛋白质合成的影响。结果表明，早孕期ｒＥＳＰ－１的分泌呈二次曲线，第３天分泌出现，第４天出现第一峰值，第５天下降，然后迅速增加，最大分泌在第７天，第８天迅速回降。其分泌量随胚胎数（或黄体数）的增加而升高，且受ＦＳＨ－ＬＨ和ＰＭＳＧ－ＬＨ的影响。ｒＥＳＰ－１使子宫细胞ＲＮＡ增加，促进子宫蛋白质合成     

龙陵黄山羊遗传多样性同功酶电泳研究

采用常规水平式淀粉胶蛋白电泳技术，对３８只龙陵黄山羊个体的３９个遗传座位的血液同功酶的多态性进行了研究。结果发现，ＡＫＰ、ＣＥＳ－Ｉ、ＥＳＤ、ＧＯＩ、ＬＡＰ、ＭＤＨ、ＭＥ和ＮＰ等８个座位具多态性，多态座位ＡＫＰＯ、ＣＥＳ－ＩＩ、ＦＳＤＡ、ＧＯＩＢ、ＬＡＰＡ、ＭＤＨＡ、ＭＥＡ和ＮＰＡ的基因频率较高；多态座位百分比Ｐ＝０２０５１，平均杂合度Ｈ＝００９０６±００２６５。结果表明，云南龙陵黄山羊与已检测的其它山羊比较，遗传多样性水平较高     

SEM科隆粉Ⅰ代替进口鱼粉饲喂蛋用种鸡试验

１材料与方法１１试验原料试验用ＳＥＭ科隆粉由辽宁科隆微生物饲料发展有限公司生产，辽宁饲料工业新技术研究所提供。ＳＥＭ科隆粉Ｉ提供的主要指标如下：粗蛋白质６４５１％，消化吸收率８４６％，复合菌总数１４９亿／克，活细胞率８５％，微生态稀土１０００毫...     

鸡新城疫、产蛋下降综合征二联油佐剂灭活疫苗的研制

１　材料和方法　１１　抗原制备　分别将ＮＤＬａｓｏｔａ株和ＥＤＳ７６种毒接种９～１１日龄鸡胚、鸭胚增殖病毒，收获感染胚液，经检测血凝价（ＨＡ）ＮＤ为≥１∶６４０，ＥＤＳ７６≥１∶２０４８０，病毒含量以尿囊腔内注射的感染量≥１０６０ＥＩＤ５０／０１ｍｌ时，可用于制苗用毒，经甲醛溶液３７°Ｃ灭活备用。１２　油相制备　取１０号白油，司本８０按一定比例混合后加硬脂酸铝少量，随加随搅拌，煮沸至透明为止，高压灭菌后备用。１３　水相制备　将灭活的Ｌａｓｏｔａ．ＥＤＳ７６尿囊液按一定比例混匀后，加入吐…     

旋毛虫肌幼虫可溶性抗原,排汇分泌抗原的电泳分析

枉文报道了应用十二烷基硫酸钠－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ），聚丙烯酰胺凝胶等电聚焦（ＩＥＦ）电泳及二维电泳（ＩＥＦ／ＳＤＳ－ＰＡＧＥ）对旋毛虫肌幼虫排汇分泌（ＥＳ）抗原和肌幼虫可溶性抗原的分析结果。肌幼虫ＥＳ抗原经ＳＤＳ－ＰＡＧＥ后用考马斯亮蓝染色蛋白质，结果显示１６条蛋白带，分子量范围２１￣８０ＫＤ，其中主带９条。ＩＥＦ电泳后分别用ＰＡＳ染多糖、考马斯亮蓝Ｒ－２５０染蛋白质、Ｎｉｌｅ＇     

用 Digoxigenin 标记核酸探针检测 EDS-76 病毒核酸的研究

用ＥＤＳ７６病毒标准ＡＶ１２７株和分离株（Ｂ９６株）感染鸭胚，提取病毒核酸，分别经ＥｃｏＲｌ和Ｐｓｔｌ双酶切，获得图形和大小相似的酶切片段。分别进行ＥｃｏＲｌ和Ｐｓｔｌ单酶切，也得到相似结果。病毒ＤＮＡ经ＥｃｏＲｌ和Ｐｓｔｌ双酶切后，与ＰＵＣ１９质粒载体重组，并转化到ＥｃｏｌｉＪＭ１０１中，筛选出一个插入片段（Ｇ片段）约为２７ｋｂ的重组质粒。用Ｄｉｇｏｘｉｇｅｍｉｎ标记ＥＤＳ７６ＤＮＡＧ片段（ＥｃｏＲｌ和Ｐｓｔｌ双酶切片段）及含该片段的重组质粒分别制备探针，对ＥＤＳ７６病毒ＤＮＡ进行斑点杂交，两种探针均为阳性，而对照组ＮＤＶ、ＩＢＶ、ＩＬＴＶ、ＩＢＤＶ正常鸭胚尿囊液的核酸为阴性。且后一种探针的敏感性高于前者，它的ＤＮＡ检出限量为４ｐｇ水平。结果表明，两种探针具有高度的特异性、敏感性。     

库姆塔格沙漠东南部胡杨液流变化及其对环境因子的响应

于2018年4月?10月(春季4月、夏季7月、秋季9月)利用PS-TDP8树木茎流监测系统对库姆塔格沙漠东南部胡杨(Populus euphratica)的液流速率变化进行监测,采用相关分析及逐步回归法分析气象因子(太阳总辐射、空气温度、风速、空气相对湿度)对胡杨液流的影响。结果表明:胡杨茎干液流日变化趋势呈几字“宽峰型”;夏季液流的启动时间最早,液流启动在06:00,秋季液流启动时间最晚,不同季节夜间存在较高液流(10.994~26.035 cm·h^?1);树干液流速率与太阳总辐射、空气温度、风速、饱和水气压差呈正相关关系,与空气湿度呈负相关关系;影响春季、秋季胡杨液流速率的主要气象因子是太阳总辐射和空气温度,夏季为太阳总辐射和空气相对湿度;不同季节胡杨液流速率与空气温度、空气相对湿度、风速、饱和水气压差的日变化响应呈顺时针环状,与太阳辐射响应呈逆时针环状;不同季节胡杨液流速率对气象因子的响应规律存在差异。这表明胡杨为了保持水分平衡应对水分亏缺的策略不同,在一定条件下(极端干旱)通过气孔调节降低水分损失。研究结果对于明确干旱沙漠环境中胡杨水分传输对环境因子的响应规律及更深入地认识胡杨适应干旱环境的水分利用策略具有重要意义。     

科尔沁沙地玉米叶面蒸腾与棵间蒸发特性

利用Dynamax包裹式茎流测量系统、小型蒸渗仪和LI-6400光合作用系统分别测定了科尔沁沙地玉米(Zeamays)的茎秆液流、棵间土面蒸发和叶面蒸腾速率.结果表明.拔节至成熟期间,科尔沁沙地玉米的逐日平均茎秆液流量为7.3～29.8 g/h,叶面蒸腾量为0.9～3.6mm,棵间蒸发量约占同期总蒸散量的20%,但灌溉...     

南农选系草地早熟禾POD同工酶的酶谱特征及与其它品种的差异

利用聚丙烯酰胺垂直板凝胶电泳技术对南农选系及16 个当前生产上应用较多的草地早熟禾品种的过氧化物酶同工酶进行了研究。结果表明,供试材料共在18 个位点表现酶带。南农选系表现出13条酶带,除与其它16个品种所共有的6 条主酶带外,还出现了其特有的7 条酶带。对不同品种POD同工酶酶谱信息进行相异性类平均聚类分析,17 个供试品种(系)聚为3 类,其中南农选系、美国品种“Merit”及其它15个品种分别各成一类。据此推测,南农选系与其它供试品种的亲缘关系相对较远,为一个相对独立的品系。     

Immediate hypersensitivity to leaf extracts of Callisia fragrans (inch plant) in a dog

Contact with members of the plant family Commelinaceae, which includes wandering jew (Commelina spp. formerly called Tradescantia spp.) and inch plant (Callisia fragrans), can cause cell-mediated contact dermatitis in dogs. However, reports of canine IgE-mediated hypersensitivity to these plants have not been published. The purpose of this study was to discover whether IgE antibodies specific for extractable components of C. fragrans could be identified in serum from a dog that had anaphylactic shock after exposure to the plant and after skin patch testing with the sap from a leaf of C. fragrans. Separate aqueous extracts of leaves and flowers of C. fragrans were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Serum from a dog with no history or symptoms of any allergies showed no specific IgE antibodies against the leaf extract. Serum from a dog with clinical symptoms of delayed, but not immediate hypersensitivity to leaf sap from C. fragrans, showed only minor IgE recognition of a single 65 k component in sap extracted from leaves harvested in summer but not in winter. However, IgE antibodies to a serum dilution of 1:200 specific for several components of the leaf extract were seen in serum from the dog that had anaphylactic shock after exposure to sap. The molecular weights of these molecules were in the range 51 k to 83 k. The bands on the immunoblots did not match with prominently stained protein bands in the gel, but instead identified molecules in a lightly stained area of the gel with diffuse bands. Testing for glycans indicated that the carbohydrate side chains of glycoproteins contributed significantly to the immunoreactivity of the putative allergens. All three dog sera failed to show any immunoreactivity against the extract from the flowers of C. fragrans.     

家蚕主要血浆蛋白质的发育变化及与其它蚕的比较研究

用SDS-聚丙烯酰腔凝胶电泳(SDS—PAGE)法,观察了家蚕不同发育阶段血液主要血浆蛋白质浓度的发育变化,比较了不同的地方性三眠蚕品种间主要血浆蛋白质组成及浓度的异同。比较家蚕与野桑蚕、蓖麻蚕、樗蚕及柞蚕血浆的电泳图谱,发现家蚕与野桑蚕的电泳图谱相似而不同于蓖麻蚕、樗蚕和柞蚕。后三者之间的电泳图谱很相似,它们不存在类似于家蚕及野桑蚕中的分子量为30KD的一组蛋白质,但也存在分子量相当于家蚕贮藏蛋白质的成分。用精制的家蚕贮藏蛋白质、30K蛋白质和卵黄磷蛋白分别制备免抗血清,并使之与五种试验蚕的血液分别进行了双向免疫扩散试验。     

陇东野生紫花苜蓿的同工酶分析

利用聚丙烯酰胺垂直板凝胶电泳技术对陇东野生紫花苜蓿及其他37个紫花苜蓿品种的过氧化物酶、过氧化氢酶和酯酶进行了分析。通过对供试材料间相似系数的计算、聚类分析和主成分分析,初步探讨了38个紫花苜蓿材料间的亲缘关系。结果表明,供试材料共表现出了24条酶带,其中有5条是陇东野生紫花苜蓿的特征酶带。38个紫花苜蓿材料的相似系数介于0.583~1.000,陇东野生紫花苜蓿与其他37个供试材料间的相似系数介于0.583~0.833,远远低于其他供试品种间的相似系数(0.625~1.000),聚类分析表明,供试材料在相似系数为0.702处可聚为2类,其中陇东野生紫花苜蓿单独聚为一类,其结果与主成分分析一致,由此可确定陇东野生紫花苜蓿与其他供试材料间的亲缘关系较远,为一相对独立的紫花苜蓿种质资源,以期通过育种途径扩大品种的遗传基础。     

Isolierung eines 97 kd Proteins aus dem Eileitersekret des Schweines mittels eines 'High-performance Electrophoresis-chromatography (HPEC)' Systems

Contents Isolation of a 97 kd porcine oviductal secretory protein using a high-performance electrophoresis-chromatography (HPEC) system The role of the oviductal secretory proteins at early stages of embryo development has yet to be elucidated and requires the biochemical characterization and isolation of these proteins. Because oviductal explant culture medium contains only very low protein concentrations, the aim of this study was to analyse and characterize cyclic specific proteins in a high protein content consisting of oviductal secretions, collected by can-nulation during the time of embryonic genome activation, and to isolate these proteins by means of simple purification steps in order to obtain the proteins for biological functional studies. Oviducts of gilts were cannulated and the secretions were collected at 24-h intervals on days 1–8 of the cycle. The protein pattern was analysed by SDS-polyacrylamide-gel electro-phoresis (Tris/tricine buffer system) and isoelectric focusing (native slab gels). Cyclic specific proteins were isolated using the ‘high-performance electrophoresis-chromatography’ system on 5% tube gels (Tris/tricine buffer system). The protein concentration of oviductal fluid increased from day 1 to 8 (1047.5 ± 252.6; 3992.5 ± 1097.3 mg/100 ml). A specific 97 kd protein was detected on days 1–3 (8.6, 6.8% of total proteins; laser densitometry), which was present at a lower level on the following days, and had reduced (1.9%) or not present by day 7–8. Its isoelectric point was 8 (2 D electrophoresis). The isolation of 97 kd protein from total oviductal secretion was possible within 4 h using preparative electrophoresis. Results indicated that, in spite of similarities to serum proteins, the cannulated oviductal fluid contains cyclic specific proteins. The amino-acid-sequence analysis of 97 kd protein should indicate whether it is the same protein detected in oviductal explant-conditioned medium. Using pre- parative electrophoresis, it was possible to isolate a specific protein from a total protein complex in very short time and this method is suitable for biological and chemical studies.     

奶牛乳腺组织亚细胞组分的双向凝胶电泳分析

利用超速离心结合梯度离心法分离亚细胞组分的技术路线,以提高奶牛乳腺组织蛋白双向凝胶电泳的分离效率。奶牛乳腺组织液氮研磨破碎后,差速离心分离成细胞核、线粒体、高尔基体、溶酶体4个亚细胞组分,Nyco-denz纯化,2-DE分离蛋白,PDQUest8.0软件分析凝胶图谱。结果显示,奶牛乳腺组织细胞经亚细胞分离后,电泳分辨率大大提高,特别是细胞核蛋白质检出效率明显提高。不同亚细胞蛋白质组电泳图谱的差异显示了其蛋白质组构成的不同。由此可见超速离心结合梯度离心是一种有效的奶牛乳腺亚细胞组分分离手段,亚细胞蛋白质组学克服了蛋白质组的一些缺陷并可将蛋白质组研究引向亚细胞水平。     

Evaluation of total protein content in tears of dogs by polyacrylamide gel disk electrophoresis.

Concentration of total proteins was measured and sodium dodecyl sulfate-polyacrylamide gel disk electrophoresis was performed on tear and plasma samples obtained from 26 healthy dogs, and the results were compared. Mean +/- SEM concentration of total proteins in tears was 0.63 +/- 0.04 g/dl, and significant effects of age or gender were not found. The protein composition of tears in dogs was complex, and bands from light and heavy chains of immunoglobulins were identified by electrophoresis.     

蒙古冰草遗传多样性的等位酶分析

采用聚丙烯酰胺凝胶电泳技术检测了内蒙古中东部地区蒙古冰草6个天然居群和2个栽培品种的遗传多样性和居群结构，12个酶位点的多态位点百分率为67.71%，基因多样性指数（He)为0.285，显示出了较高的遗传多样性，从遗传多样性的分布格局来看，8个居群的遗传分化系数Gsr=0.129，表明在总的遗传变异中，只有12.9%的变异存在于居群间，而87.1%的变异存在于居群内，同时，6个天然居群的总基因多样度（HT=0.439)和基因分化系数（GST=0.032)也大于2个栽培品种的相应值（HT=0.423,GST=0.010)。UPGMA聚类分析的结果表明，蒙古冰草居群间的遗传分化与生态环境因子间有着密切的相关性。