健康蒙古马关节滑液中细胞成分的研究

在马骡肢蹄病中,较为多见的是四肢关节炎症性和非炎症性疾患。而炎症的病理变化过程又必然会引起滑液中细胞成分的相应改变。因此,研究正常关节滑液中的细胞成分,对于了解病理性的关节滑液中细胞成分的变化规律,以及对临床某些关节疾病的早期确诊和疗效观察等都有一定的实践意义。     

健康蒙古马关节滑液中细胞成分的研究

在马骡肢蹄病中,较为多见的是四肢关节炎症性和非炎症性疾患。而炎症的病理变化过程又必然会引起滑液中细胞成分的相应改变。因此,研究正常关节滑液中的细胞成分,对于了解病理性的关节滑液中细胞成分的变化规律,以及对临床某些关节疾病的早期确诊和疗效观察等都有一定的实践意义。     

关节滑液的研究 Ⅰ.马关节滑液中朊的电泳分析(初报)

前言大家畜的关节疾病早已引起我们极大的重视。已知,关节疾病中炎症性的疾患最为多见,而炎症性的病理过程又必然会引起滑膜及滑液的相应变化,因而研究正常的及病理性的关节滑液的变化,对确定某些关节疾病的病性及诊断,非但有重要的理论价值,也有现实的临床意义。     

健康驴关节滑液中细胞及化学成分的研究

本文对健康驴关节滑液中的各种细胞成分、蛋白质含量、碱性磷酸酶活性、酸性磷酸酶活性、乳酸脱氢酶活性、谷草转氨酶海性和粘蛋白含量进行了测定，为临床诊断和治疗关节疾病提供了依据。     

关节滑液的主要成分及临床检查的意义

关节滑液的主要成分及临床检查的意义冯新畅王林安（东北农业大学动物医学系·哈尔滨·１５００３０１关节滑液的来源关于关节滑液的来源问题，有五种学说，即：分泌说、渗透说、磨损变性说、弥散平衡说、透析说［１］。分泌说认为滑液是由滑膜细胞及腺体分泌产生的；渗透...     

马四肢关节滑液蛋白的电泳分析

浆液性关节滑膜炎是马骡的常见病、多发病。本病发生后,其症状以关节囊内蓄积多量浆液性渗出物为主要特征。因此,研究关节滑液的性状、成分及其变化规律,对本病的诊断和治疗有着极为重要的意义。     

馬、牛关节滑液細胞計数法及其正常数值

关节滑液是由关节滑膜分泌出来的一种液体,它不但在关节运动时能起着与关节軟骨相似的緩冲作用,而且,氏还认为,由于滑液內含有淋巴球,組織細胞,故滑液在正常情况下还有着一定的防卫功能。因此当关节发生病理改变时,不但会引起滑液的物理,生化的变化,同时也不可能不会引起細胞学的变化。临床实践也証实了     

马关节穿刺术

前言滑液在营养关节软骨和润滑关节软骨中起着重要的作用。通过对反映其微环境的滑液的理化性质的分析,有助于关节疾病的诊断和治疗。本文着重讨论关节穿刺、滑液分析的技术和应用,特别是关于临床实践方面的应用。     

一例青年蛋鸡滑液囊支原体病的诊断

<正>鸡滑液囊支原体病,又称传染性滑膜炎,它是鸡的一种急性到慢性的疾病,该病主要侵害鸡的各个关节,特别是龙骨的滑液囊、跗关节的滑液囊和关节周围的腱鞘,从而引起关节部位形成渗出性滑膜炎和腱鞘炎。本文通过对某养殖场青年鸡呈现腱鞘炎、滑膜炎和骨关节炎为主疾病的诊断,确诊为滑液囊支原体感染,现报道如下。     

猪滑液支原体关节炎的病因、诊断和治疗

猪滑液支原体关节炎又被称为猪滑液囊支原体病,是由滑液囊支原体感染引发的一种以关节僵直,不能正常行走为主要特征的临床综合症。滑液囊支原体广泛存在于母猪的鼻腔和咽喉当中,通常不会表现出明显的致病性,大多呈现隐性发病经过。猪群的年龄相对较小,并且抗病能力低下之后,滑液囊支原体才会大量繁殖,产生致病性,危及到猪群的健康生长。通常情况下,滑液囊支原体会对4～8周龄的仔猪造成严重威胁,该种传染性疾病的传播途径还不是很明确,但在某些猪群当中猪在12周龄就会通过鼻腔或者咽喉感染滑液囊支原体引发发病。大量研究结果表明,猪滑液囊支原体关节炎的发生流行与病原的传播速度和养殖密度以及环境卫生有密切联系。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.