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1.
W. Heijbroek 《Euphytica》1977,26(2):257-262
Summary Within Beta vulgaris and B. maritima origins some partial resistance to beet cyst eelworm was found which could be raised to a very limited extent by selection. However after the second backcross to commercial sugar beet varieties and successive selection of the inbreds this resistance was lost. It was demonstrated that in the rootsystem of resistant plants as much nematodes penetrate and develop as in susceptible ones but the ratio between males and females is different. It was therefore quite probable that this resistance is polyfactorial and merely recessive.  相似文献   

2.
Summary An increasing number of genetically engineered cultivars of several crops is being experimentally released into the environment. In future, crops with new transgenic traits will probably play an important role in agricultural practice. The long-term effect of transgenes on community ecology will depend on the distribution and establishment of transgenic plants in the wild, on the sexual transfer of their new genes to the environment and on the potential ecological impact of the transgenic trait. The starting point was the use of transgenic sugar beet lines, Beta vulgaris subspec. vulgaris var. altissima DÖLL (Helm 1957), with transgenes coding for rhizomania and herbicide (BASTA®) resistance. The first two questions to answer were: Can the transgenes be transferred via pollen to wild beets, Beta vulgaris subspec. maritima (L.) ARCANG. or cultivated relatives such as red beet or spinach beet and are they expressed in the hybrids? Can transgenes be monitored in young Beta vulgaris-hybrids? The experimental transfer of transgenes was conducted in 1993 at a field location in northern Germany. The beets were hand-pollinated with transgenic pollen. In a non destructive biotest, the hybrid seedlings were tested for herbicide resistance. Transgenic plants showed no noxious phenotypic effects whereas control plants developed leaf necroses. All herbicide resistant hybrids within the biotest were assumed to be transgenic.  相似文献   

3.
Summary Clones derived from Beta vulgaris and Beta maritima were assayed for their ability to detect restriction fragment length polymorphisms (RFLPs) in different beet accessions. The clones able to detect polymorphism were used as genetic markers to assess the degree of genetic variation existing between and within different species of the genus Beta. The data support the current taxonomy of the Beta vulgaris section, while the great genetic similarity found between Beta webbiana and Beta procumbens indicates that they could belong to the same species.Enough variation was found between Beta vulgaris cultivars, allowing the isolation of a sufficient number of genetic markers for the construction of detailed genetic maps.  相似文献   

4.
Summary Successive mass selections were made in sugarbeet varieties (Beta vulgaris L.) and in B. vulgaris × B. maritima L. hybrids for tolerance to wilting caused by beet-cyst eelworm attack. The selected lines showed improved tolerance to wilting, but no evidence of resistance to eelworm infection was demonstrated. By repeated back-crossing of the selected lines with the commercial varieties and concurrent selection root yield could be improved without substantially decreasing the tolerance to eelworm attack. These results indicate that tolerance is partly conditioned by complete and incomplete dominant genes.  相似文献   

5.
Paul  H.  van Deelen  J. E. M.  Henken  B.  de Bock  Th. S. M.  Lange  W.  Krens  F. A. 《Euphytica》1990,48(2):153-157
Summary Hairy roots, induced by Agrobacterium rhizogenes, were obtained of nematode susceptible beet plants (Beta vulgaris) and of the nematode resistant alien monotelosomic addition AN5, carrying a telosome from B. patellaris. The additional telosome was found to be stably present in vitro in the roots of AN5. The hairy root cultures were inoculated with larvae of the beet cyst nematode Heterodera schachtii. On the root culture of AN5 significantly less cysts developed than on the other root cultures. These results indicate that the resistance to the beet cyst nematode is expressed in the roots after transformation and can be monitored under in vitro conditions.  相似文献   

6.
Summary Experiments were carried out for adding the chromosome carrying resistance to beet root nematode (Heterodera schachtii) from the wild Beta species of the section Patellares (B. procumbens, B. webbiana and B. patellaris) to the genome of B. vulgaris. Preliminary experiments indicated that crosses between the wild species and B. vulgaris cultivars of the mangold type yielded on average more viable F1 hybrids than crosses with sugar and fodderbeet. However, crossability varied strongly between individual parental combinations. It was concluded that most types of B. vulgaris can be hybridized with the wild species of the section Patellares if a sufficient number of pair-crosses is made. Crosses between diploid cultivars or species of the section Vulgares and diploid wild species of the section Patellares yielded many hybrids which, however, were highly sterile. From crosses between tetraploid B. vulgaris and the wild species a great number of viable allotriploid and allotetraploid hybrids was obtained. In the backcross progenies of allotriploid hybrids 26% alien monosomic additions occurred, of which 4.1% carried the resistance bearing chromosome of B. procumbens or B. patellaris. The programme will be continued by sereening progenies of the resistant monosomic addition plants for the occurrence of resistant disomic introgression products.  相似文献   

7.
Summary A population of 2xms sugar beets was crossed with 4x Beta lomatogona F. et M. The 3x F1-plants were male sterile and were backcrossed with 2x and 4x sugar beets and multiplied without pollination as well. After the 1st backcross mainly 3x apomict types arose again and, among others, a small number of successful 4x backcrosses. After pollination by 4x sugar beets this 4x F1 B1 produced. besides predominatly apomictically multiplied 4x plants, also about 7% haploid 2x hybrids. The latter probably possess 1 genome from B. vulgaris and 1 genome from B. lomatogona. In the meiosis of the PMC's a certain amount of homeology between a number of chromosomes of both species could be established. The amphihaploid hybrids can be used as breeding parents for the creation of types in which introgession can occur. During hybridization in addition to 2x and 4x B. vulgaris types a number of 2x-, 3x-, 5x- and 6x-hybrids arose. This is presumably caused by the presence of gametes with the somatic number of chromosomes and the occurrence sometimes of haploid apomictic multiplication.The presence of large numbers of bolters in the F1 and F1 B1 suggests that the bolting tendency of both species is based on different genes.  相似文献   

8.
Summary Leaf discs from sixBeta vulgaris lines and threeB. maritima accessions were tested for their morphogenic responses in Murashige and Skoog medium supplemented with 0.25 mg/l benzyladenine (BA). Direct shoot formation took place in two genotypes:B. vulgaris line TA33-MS andB. maritima acc. France. Rooting of differentiated shoots was achieved by culturing the shoots on a medium containing 0.2 mg/l indolebutyric acid (IBA). Our results indicate that genotypes differ in the morphogenic potential of leaf explants.  相似文献   

9.
Summary Beet cryptic viruses occur differentially in cultivars of chard (only BCV 1) and red beet (only BCV 2). As landraces of these crops and wild beets can contain both of the viruses, the differential occurrence in cultivars should indicate founder effects in the course of evolution of chard and red beet. Consequently, the genetic base of those crops should be considered as narrow. On the basis of the presence or absence of the viruses in beet plants within the populations conclusions can be drawn concerning the uniformity of cultivars maintained in the collections, the characterization of chard and beet landraces and some evolutionary tendencies within the variable Beta vulgaris-complex.  相似文献   

10.
Summary Appropriate definitions of, and methods of measuring, resistance are sought using Phaseolus vulgaris, a major source of protein in Latin America, and its most important pest, Empoasca kraemeri. The concepts of general vigour and narrow-sense resistance are defined: these two components make up broad-sense resistance. The validity of these definitions, which depend on the inclusion of tolerance as a resistance mechanism, is discussed. In order to distinguish the components, 38 genotypes of P. vulgaris were grown with and without insecticidal proctection, in three replications in a split-plot design, with three planting dates. Seed yield per plant, and a visual score of damage symptoms or vigour, were measured. Three methods of estimating narrow sense resistance are considered: 1) the genotype × insecticide interaction effect in a split-plots analysis, 2) the yield of each genotype unprotected (Yu) divided by its yield protected (Yp). and 3) the deviation of each genotype from a regression of Yu on Yp. The third method overcomes some objections to the first two, and can be applied to damage and vigour scores as well as to yields. The 38 genotypes differed in narrow-sense resistance according to all three methods, and it appears that in practice Methods 2 and 3 were both valid. They also differed in general vigour. Breeders working with many pests and crops have found that low damage and high yield in the presence of the pest are effective selection criteria. The work reported here supports this view.  相似文献   

11.
Summary Monosomic additions of Beta vulgaris x B. procumbens with resistance to beet cyst nematode (Heterodera schachtii Schm.) were used for the production of resistant diploids through incorporation of the B. procumbens chromosome fragment bearing the resistance gene(s) into one of the sugar beet chromosomes. The heterozygotes obtained accordingly were selfed for producing homozygotes. These homozygotes differed morphologically from commercial sugar beet varieties, but produced reasonable amounts of pollen. Female transmission of resistance was 100%, whereas male transmission, apart from some exceptions, was more than 90%. The number of hypersensitivity reactions to penetrated larvae was related to the degree of susceptibility. Larval development was severely retarded in the resistant plants, preventing most of them to produce cysts. If cysts were formed, their content was considerably less as compared to those in the susceptible plants.  相似文献   

12.
Summary Inheritance of resistance to beet necrotic yellow vein virus (BNYVV) was studied in segregating F2 and backcross families obtained from crosses between resistant plants of the sugar beet selection Holly-1-4 or the wild beet accession Beta vulgaris subsp. maritima WB42 and susceptible parents. Greenhouse tests were carried out, in which seedlings were grown in a mixture of sand and infested soil. Virus concentrations of BNYVV in the rootlets were estimated by ELISA. To discriminate resistant and susceptible plants, mixtures of normal distributions were fitted to log10 virus concentrations, estimated for segregating F1, F2 and BC populations of both accessions. The hypothesis that Holly-1-4 contained one single dominant major gene was accepted. For WB42, results fitted with the hypotheses that resistance was based on either one (or more) dominant major gene(s) showing distorted segregation, or two complementary dominant genes, which are both required for resistance. Resistance from WB42 appeared to be more effective against BNYVV than resistance from Holly-1-4.This research was carried out as part of a PhD study at the Graduate School Experimental Plant Sciences (EPS), Department of Virology, Wageningen, The Netherlands  相似文献   

13.
Summary The O-type lines (N-plasm) of sugar beet (Beta vulgaris L.) proved to have a consistently lower peroxidase activity (49.2–62.7%) than their male sterile (MS) equivalents (S-plasm).Similar differences in peroxidase activity (45.4–56.3%) were found when O-type lines were compared with unrelated MS lines. They were also observed in different parts of the plant (cotyledons, hypocotyl + radicle, true leaves) and were reproducible.The fertile lines could be grouped by their level of peroxidase activity in categories of either S-plasm or N-plasm.The peroxidase activity test could be used for selection of N-plasm lines from fertile sugar beet populations and for selection of MS and O-type lines of higher stability.  相似文献   

14.
Halo-blight is an important worldwide bacterial disease of common bean (Phaseolus vulgaris L.) caused by Pseudomonas syringae pv. phaseolicola. Nine races of the pathogen and five race-specific resistance genes have been previously described. However, a quantitative response to this pathogen has also been described. The objective of this study was to identify halo-blight resistance loci linked to molecular markers that could be used in resistance breeding. Chromosomal regions related to race 5 halo-blight resistance were localized on a genetic map of RAPD and AFLP molecular markers and constructed by the analysis of a “Jules” × “Canela” F2 progeny. “Jules” shows quantitative resistance to halo-blight and “Canela” is a very appreciated but susceptible Spanish bean landrace. Two QTL for resistance to halo-blight were mapped in two linkage groups. There were four large groups, with 14–22 molecular markers each, five with 4–8 markers each, and three with 2 or 3 markers each.  相似文献   

15.
Summary Breeding of Phaseolus vulgaris L. for resistance to common bacterial blight (CBB) can be done with visual evaluations of symptoms to distinguish broad resistance classes, but a more quantitative measure was needed for genetic studies of resistance. A novel method of evaluation was developed by quantifying Xanthomonas campestris pv. phaseoli (XCP) in bean leaf tissue infected with CBB using a 32P-labeled probe and densitometric analysis of hybridization signals. Quantification of bacterial populations using the probe was highly correlated (r=0.98) with the number of colony forming units (CFU) from plate counts of the same leaf samples. The probe was used to follow XCP population dynamics on susceptible (BAT 41) and resistant (OAC 88-1) bean genotypes. OAC 88-1 supported a maximum XCP population which was approximately tenfold less than BAT 41. The probe was also used to study an F2/F3 population segregating for resistance. Narrow sense heritability estimates were less for resistance measured on the basis of bacterial populations (0.18–0.26) than on visual scores of symptoms (0.29–0.38). The anticipated response to selection for CBB resistance would be less based on bacterial numbers than based on symptom expression in this population. In breeding for resistance to CBB, selection based on visual symptoms combined with measurements of XCP populations using a DNA probe can be used to develop bean genotypes that are both resistant to symptom development and bacterial multiplication.Abbreviations CBB common bacterial blight - CFU colony forming units - XCP Xanthomonas campestris pv. phaseoli  相似文献   

16.
A. K. Singh  S. S. Saini 《Euphytica》1980,29(1):175-176
Summary Angular leaf spot (Isariopsis griseola Sacc.) is a serious disease of French bean in the hills of India and 40 to 70 per cent of the green pods are damaged and rendered unmarketable. Crosses were made between PLB 257, (Phaseolus coccineus L.), a red flowering pole tope, resistant to angular leaf spot, and Contender (Phaseolus vulgaris L.), a highly susceptible commercial cultivar. Studies of the F1, F2, and F3 progenies indicated that PLB 257, carries a recessive gene imparting resistance to angular leaf spot.  相似文献   

17.
Summary Fifty-four land races of dry beans (Phaseolus vulgaris), indigenous to areas of Brazil where anthracnose (caused by Colletotrichum lindemuthianum) is a common problem, were evaluated in field nurseries for partial resistance to race Brazilian 1 (B1) of C. lindemuthianum using symptom severity classes (SSC) from 0 to 6. Plants were selected if symptoms were present and the SSC was less than the 95% confidence interval of the mean SSC of the susceptible cultivar Carioca. S1 progeny from selected plants were evaluated in air-conditioned chambers for partial resistance to races B1, delta, and kappa of C. lindemuthianum. Of 246 S1 families evaluated, 145 families were partially resistant to one or two of the races [symptoms present, but S1 family mean significantly (p<0.05) less than the mean of Carioca] and susceptible to the third. Six families were partially resistant to all three races. The remaining families were either susceptible or segregated for reaction to race B1. Partial resistance to C. lindemuthianum showed race specificity in the air-conditioned chambers and field nurseries.  相似文献   

18.
Snap bean (Phaseolus vulgaris L.) cultivar, Widusa, was crossed to Michigan Dark Red Kidney (MDRK), Michelite, BAT 93, Mexico 222, Cornell 49–242, and TO cultivars to study the inheritance of resistance to anthracnose in Widusa. The segregation patterns observed in six F2 populations supported an expected 3R:1S ratio suggesting that Widusa carries a single dominant gene conditioning resistance to races 7, 65, 73, and 453 of Colletotrichum lindemuthianum, the causal organism of bean anthracnose. Allelism tests conducted with F2 populations derived from crosses between Widusa and Cornell 49–242 (Co-2), Mexico 222 (Co-3), TO (Co-4), TU (Co-5), AB 136 (Co-6), BAT 93 (Co-9), and Ouro Negro (Co-10), inoculated with races 7, 9, 65 and 73, showed a segregation ratio of 15R:1S. These results suggest that the anthracnose resistance gene in Widusa is independent from the Co-2, Co-3, Co-4,Co-5, Co-6, Co-9, and Co-10 genes. A lack of segregation was observed among 200 F2 individuals from the cross Widusa/MDRK, and among 138 F2 individuals from the cross Widusa/Kaboon inoculated with race 65, suggesting that Widusa carries an allele at the Co-1 locus. We propose that the anthracnose resistance allele in Widusa be named Co-1 5 as Widusa exhibits a unique reaction to race 89 compared to other alleles at the Co-1 locus. RAPD marker A181500 co-segregated in repulsion-phase linkage with the Co-1 5 gene at a distance of 1.2 cM and will provide bean breeders with a ready tool to enhance the use of the Co-1 5 gene in future bean cultivars.  相似文献   

19.
Summary Some haploids isolated among the progeny from crosses involving several genotypically different motherplants of Beta vulgaris L., were used for an investigation on chromosome morphology of the genus Beta.The length of both the long and short arms proved to vary considerably for each chromosome, so that a representative idiogram of the 9 chromosomes could not be made. Staining the chromosomes according to the BSG-technique revealed only bands in the centromere regions. However, one chromosome with a narrow band in the short arm could be identified, which was supposed to be the nucleolar chromosome. The C-bands presumably correlate with the heterochromatic blocks as observed in mitotic and meiotic (pachytene) prophase.This study was part of a project on cytogenetics of interspecific hybrids resistent to beet cyst eelworm, supported by the Institute of Rational Sugar Production,Bergen op Zoom, the Netherlands.  相似文献   

20.
Yu  M. H. 《Euphytica》1984,33(3):633-640
Summary Fifty-two accessions of the section Patellares wild beet (including 26 Beta patellaris Moq., 13 B. procumbens Chr. Sm and 13 B. webbiana Moq.) and 14 progeny families were selected and tested against sugarbeet cyst nematode, Heterodera schachtii Schm. All Patellares species tested were highly resistant, but not immune, to the development of H. schachtii, after infection. This is the first report that mature female nematodes developed in the roots of B. webbiana plants. The occasional development of nematode cysts in roots of juvenile wild beets was, however, not a heritable genetic factor.Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products or vendors that may also be suitable.  相似文献   

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