Replacement of live feed by formulated feed: effect on the growth and spawning performance of Siamese fighting fish (Betta splendens,Regan, 1910)

Partial replacement of live feed (LF) with formulated feed (FF) was performed in Betta splendens to evaluate their growth, survival and reproductive performance. Three hundred B. splendens fry of uniform size (mean weight 0.19 g±0.01) were equally distributed in five treatment groups with each of three replicates in glass aquaria of 35 L capacity. Fish were offered diets of different ratio of LF and FF like T1 (control) – 100% LF; T2 – 75% LF, 25% FF; T3 – 50% LF, 50% FF; T4 – 25% LF, 75% FF and T5 – 100% FF. Highest (P<0.05) body weight gain (% BWG) (67.1±1.03) and specific growth rate (2.34±0.02) were observed in the T2 group. Best feed conversion ratio was also found in T2 (2.40±0.11), which was similar to T1 and T3. The highest survival rate (%) was recorded in T1, T2 and T3 groups (97±1.7), which was similar to T4 and the lowest in T5 (49±2.0). The highest gonadal weight (0.12 g±0.01) and gonadosomatic index (15.17±0.50) was observed in the T3 group during the first sampling after 63 days. No significant changes in spawning performance were observed in the second sampling after 21 days after first sampling. However, T4 group registered the highest fecundity and fertilized eggs at the end of 105 days of experiment. From the present study, it concludes that LF can be successfully replaced to the extent of 25% by FF without any detrimental effect on the growth, survival and spawning performance of B. splendens.     

The effect of different diets on the growth and mortality of juvenile pikeperch (<Emphasis Type="Italic">Sander lucioperca</Emphasis>) in the transition from live food to formulated feed

The efficiencies of four feeding methods were compared in the transition period from live food to dry feed in pikeperch (Sander lucioperca). Pond-nursed fry were trained to artificial feed without a transition (Group P) and with a transition, using a combination of dry feed and different kinds of live food—Chironomus larvae (Group C), Tubifex (Group T) and Daphnia (Group D)—during a 12-day-long trial. The ratio of dry feed for the transition groups was increased from 0 to 100% in 3-day intervals. The highest specific growth rates and condition factors were detected for Group C (4.2%, 1.46 ± 0.5) and Group T (4.2%, 1.48 ± 0.6), respectively. The survival percentages of the groups were as follows: 86.7 ± 9% (Group C), 78 ± 4% (Group T), 52 ± 1.4% (Group D) and 41 ± 9% (Group P). The rate of cannibalism ranged between 6.3 and 13.7% during the transition period. Larger-sized fish seemed to learn more rapidly to accept dry feed than their smaller counterparts.     

Evaluation of live duckweed,Wolffia globosa as an allochthonous feed for Labeo rohita fry during nursery rearing

A 40 days feeding trial with Labeo rohita (rohu) fry was conducted in six outdoor cemented tanks (5m × 4m × 1 m; 6–8 cm soil base) to establish the relative impact of on‐farm grown live Wolffia globosa (a duckweed with crude protein of 28.57%) and a formulated iso‐nitrogenous feed (crude protein 28.37%) on growth performance, survival, health and overall quality of fingerlings. The experimental tanks were dried, limed at 250 kg/ha, filled with ground water and subsequently applied with cow dung (soaked overnight) and mustard oil cake at 1,000 kg/ha and 50 kg/ha respectively. The fry were stocked at 0.3 millions/ha in six prepared tanks, and fed live Wolffia (T1) and formulated feed (T2). Results of the study indicate better performance (p < 0.05) of fry in terms of final mean weight (7.83 g), length (8.82 cm), daily growth index (6.51 g %/day) and survival (89.5%) than those fed on formulated feed. Furthermore, significantly improved (p < 0.05) feed utilization parameters viz. apparent feed conversion ratio (AFCR), apparent protein conversion efficiency (APCE), apparent protein efficiency ratio (APER); haematological parameters viz. total erythrocyte count (TEC), total leucocyte count (TLC), packed cell volume (PCV), plasma glucose and protein of fry were also observed in T1 (live Wolffia). Overall findings from this study hints towards the possible utilization of on‐farm grown live Wolffia as a suitable nutrient source for fry rearing of L. rohita.     

Growth performance of pengba,Osteobrama belangeri (Valenciennes, 1844) and water quality changes during fingerling rearing with varied stocking density in large outdoor concrete tanks

Fingerling rearing of pengba, Osteobrama belangeri, was conducted at varied densities for 90 days in 12 outdoor concrete tanks (10 m × 5 m × 1 m). The tanks, grouped into four triplicated treatments, were stocked with fry at 20, 30, 40 and 50 fry m^‐3; and designated as control, T‐1, T‐2 and T‐3 respectively. Tanks were filled up to 90 cm depth. Evaporation loss compensated fortnightly. Fish sampling and monitoring of water quality parameters done at fortnight intervals. Survival varied between 71.5% and 84.0% in treatments. Both survival and total length significantly reduced with increasing densities from control to T‐2 (p < .05), while no differences were observed between T‐2 and T‐3 (p > .05). Similarly, significant reductions in final body weight and specific growth rate were observed with increased densities and these values further reduced in T‐3 than T‐2 (p < .05). Such results corroborated the inverse relationship between stocking density and growth. Gross biomass yields in two higher densities (T‐2 and T‐3), despite their lower survivals, were significantly higher than the two lower densities. The numbers of fingerlings harvested were 35, 74 and 112% higher than the control in T‐1, T‐2 and T‐3 respectively. The lowest size obtained even at the highest density (T‐3) was of 7.0 ± 1.0 cm and 3.99 ± 0.17 g, which can be considered as ideal grow‐out stocking size of pengba. Therefore, the study showed feasibility of stocking up to 50 fry m^‐3 for rearing from fry to fingerling stage. However, the other lower densities may be used in case of requirement of larger fingerlings.     

Determination of the muscle residue levels and withdrawal time of florfenicol in healthy and experimentally Lactococcus garvieae infected rainbow trout (Oncorhynchus mykiss,Walbaum 1792) grown in the Black Sea water

The aim of this study was to determine, muscle residue level (MRL) and withdrawal time (WT) of florfenicol (FF), in healthy and infected rainbow trout (Oncorhynchus mykiss) with Lactococcus garvieae. Fish were divided into the four groups, Group I (Infected fish fed with FF free diet, n = 10), Group II (Healthy fish fed with FF free diet, n = 10), Group III (Infected fish fed with FF containing diet, n = 80) and Group IV (Healthy fish fed with FF containing diet, n = 80). Fish in Group I and Group III were infected with 1.8×10⁶ cfu/ml Lactococcus garvieae by immersion. FF containing diet was applied fish (10 mg kg^‐1 day^‐1) in Group III and IV for 10 days. FF residues in fish muscles were analysed by validated HPLC method. The limit of detection (LOD) and limit of quantification (LOQ) values of the method were determined to be 0.31 μg/g and 0.64 μg/g, respectively, and the recovery was 92.61%. As a result of the experiments, there was no significant difference between the infected and healthy group in term of MRLs. However, in both groups, it was determined that third‐day values were below MRLs (1,000 μg/kg). Also, the WT values were lower than the 500°C‐day values reported in the EC commission decision in both groups and were determined as 14.5 and 14.12 (water temperature mean 18°C ± 1) per day, respectively.     

Effects of dietary probiotic Lactobacillus plantarum and whey protein concentrate on the productive parameters,immunity response and susceptibility of Nile tilapia,Oreochromis niloticus (L.), to Aeromonas sobria infection

Immunostimulatory feed supplements have an increasingly interest in aquaculture management. Generally, an individual supplement was used in fish diets but it is expected that the use of multi‐supplements may show synergistic enhancements in fish performance, health, and immunity. Therefore, the present investigation was carried out to evaluate the use of dietary probiotic Lactobacillus plantarum and whey protein concentrate (WPC) in practical diets for Nile tilapia, Oreochromis niloticus. Hence, probiotic L. plantarum, WPC and their mixture were incorporated into a basal fish diet (300 g/kg crude protein) as follows: T1 = a basal control diet, T2 = a basal diet containing L. plantarum, T3 = a basal diet containing 1.0 g WCP/kg diet and T4, T5 or T6 = basal diets containing probiotic L. plantarum + 1.0, 2.0 or 3.0 g WCP/kg diet, respectively. Fish (15.2 ± 0.6 g) were fed on one of the tested diets up to apparent satiation twice a day for 60 days. After that, fish were intraperitoneally injected with pathogenic bacteria Aeromonas sobria and fish mortality was observed for 10 days postchallenge. Fish growth and feed intake were significantly improved by dietary probiotic L. plantarum (T2) and/or WPC (T3) over the control group (T1), and highest fish performance was observed in T5–T6 fish groups. Similarly, highest values of haematocrit, glucose, total proteins, albumin, and globulin were significantly observed in T5–T6 fish groups. Likewise, fish fed dietary probiotic L. plantarum (T2), WPC (T3), and their mixture (T4–T6) showed antioxidants and immune‐stimulating activities better than the control group. Fish fed the control diet were more susceptible to A. sobria infection showing highest fish mortality (75.0%). Meanwhile, dietary probiotic L. plantarum (T2), WPC (T3), and their mixture (T4–T6) enhanced significantly the fish resistance to A. sobria infection resulting in maximum values of relative percent of fish survival (73.3%–80.0%) in T5–T6 groups. The present investigation recommended the use of probiotic L. plantarum with 2.0 g WPC/kg diet to improve the growth, antioxidant, immunity responses and tolerance of Nile tilapia to A. sobria infection.     

Effects of common carp (Cyprinus carpio Linnaeus, 1758) and the African catfish (Clarias gariepinus Burchell, 1822) on growth and reproductive performance of native tilapia Oreochromis shiranus (Boulenger, 1896)

A study was conducted to assess the effects of common carp and the African catfish on growth and reproduction of the native tilapia Oreochromis shiranus in Malawi. The study was done from 1 May to 1 October 2018 at the National Aquaculture Centre (NAC), Zomba. Four triplicated treatments were used: O. shiranus + carp (T1), O. shiranus + catfish (T2), O. shiranus + carp + catfish polyculture (T3) and O. shiranus monoculture (T4). Fish were stocked at uniform density of 0.8 fish/m². Data collection was done once every month. Results showed that O. shiranus mean weight gain (%), specific growth rate (% body weight/day), average daily gain (g fish^?1 day^?1) and condition factor (g/cm³) were highest in T3 and lowest in T1 and T4 treatments. T3 had better water quality regime and higher tilapia biomass than T1 and T4 treatments. Tilapia fry production (no. fry pond^?1 day^?1) was highest in T4 but did not significantly differ (p > .05) between T2 and T3 treatments. It is concluded that the farming of common carp in aquatic ecosystems containing the African catfish may not adversely affect growth and reproduction of O. shiranus and that the polyculture of the African catfish, common carp and tilapia can be adopted to mitigate the potential adverse effects of carp on the environment and improve tilapia growth.     

Comparison of mudcrab‐based brackishwater polyculture systems with different finfish species combinations in Sundarban,India

To compare production and economic performance of polyculture systems with different species combinations, a 210‐day trial was carried out. In the first combination (T1), milkfish (Chanos chanos) and mudcrabs (Scylla serrata), and in the second (T2), mullets (Mugil cephalus, Liza tade and Liza parsia at 0.5:0.5:0.5 ratio) and mudcrabs were stocked keeping fish and mudcrabs at 15,000 numbers per ha, respectively, in both treatments. The finfish were fed floating pellet at 2%–3% and mudcrabs were fed fresh and farm made feed at 5%–8% body weight. Growth parameters of mudcrabs were similar in both T1 (407.64 ± 105.78 g) and T2 (418.89 ± 105.24 g), with no significant differences. Among finfish, M. cephalus attained highest final body weight, 241.55 ± 26.44 g followed by milkfish, 200.46 ± 11.82 g whereas lowest growth noticed in L. parsia (63.69 ± 6.62 g). Length–weight analysis of fish indicated negatively allometric growth (b < 3) for grey mullets, parsia and milkfish while L. tade recorded perfect cube low (b = 2.99). Male mudcrabs recorded positive allometric (b = 3.3) and female crab exhibited negative allometric growth (b = 2.68). The total productivity was 4,533 and 3,694 kg/ha with mudcrab contributes 53.69% and 60.56% to the total productivity in T1 and T2 respectively. The economic analysis indicated benefit–cost ratio (BCR) of 1.57 and 1.73 in T1 and T2 respectively with 10% and 35% insignificant increase (p > 0.05) in BCR and profit per kg, respectively, in T2 compared with T1. The study elucidates polyculture of mudcrabs with finfish can be a taken up as a profitable venture for sustainable diversification of brackishwater farming in Sundarbans.     

Length–weight relationship and growth performance of different life stages of hatchery‐produced magur,Clarias magur (Hamilton, 1822)

Length–weight relationship (LWR) of 661 numbers of hatchery‐produced magur, Clarias magur of all the life stages such as hatchlings, fry, fingerlings, juveniles and adults, was studied. The LWR of all the life stages of magur was estimated and the equations are as follows, hatchlings W = 0.0052*L^1.282 (r² = 0.8784), fry W = 0.0088*L^3.028 (r² = 0.8113), fingerlings W = 0.0065*L^3.226 (r² = 0.8906), juveniles W = 0.0028*L^3.420 (r² = 0.8175) and adults W = 0.0017*L^3.491 (r² = 0.8310). A positive allometric growth (b > 3) was observed in all the life stages except hatchlings. The annual growth rate of magur was observed with the body length and weight of 24 ± 1 cm and 115 ± 17 g, respectively, under the commercial production system. This is the first kind of study that describes about the LWR and growth performance of all the life stages of hatchery‐produced C. magur catfish from hatchlings to adults under commercial production system. This study will be more useful to the hatchery managers and fish growers to optimize the resources and husbandry practices to improve the growth, survival, and biomass production.     

Effect of dietary phospholipid sources and levels on growth performance,enzymes activity,cholecystokinin and lipoprotein fractions of rainbow trout (Oncorhynchus mykiss) fry

This study was conducted to evaluate the effect of soybean lecithin (SBL) and chicken egg lecithin (EGL) on growth performance; pancreatic and intestine enzymes, cholecystokinin (CCK) and lipoprotein fractions of rainbow trout, Oncorhynchus mykiss (Walbaum) fry. Seven isonitrogenic and isolipidic diets were prepared by replacing 0 (as a control), 2%, 4% and 6% soybean and chicken egg lecithin instead of soybean oil in diets. Trout fry with initial average weight of 120 ± 4.08 mg, were randomly distributed in 21 90 L tanks. Triplicate groups (165 fish for each tank) of fry were fed each diet to visual satiation at six meals per day for 40 days. Dietary supplements of 2% and 4% chicken egg lecithin led to increase in fry final weight, weight gain and specific growth rate suggesting that moderate PL levels are needed during this stage of rainbow trout. Also 2% chicken egg lecithin caused significantly higher activity of amylase, lipase, phospholipase A₂, alkaline phosphatase (AP), aminopeptidase‐N (AN) and lower activity of leucine alanine peptidase (leu‐ala) in the digestive tract of fry than those in control group. Furthermore, fry fed 2% chicken egg lecithin had a higher ratio of AN/leu‐ala and AP/leu‐ala than that in the control group. Chicken egg lecithin increased significantly cholecystokinin (CCK) and chylomicron fractions of fry compared to control group. It is noteworthy that soybean lecithin had moderate effect on final growth, weight gain and specific growth rate of fry and induced significantly higher activity of amylase, lipase, phospholipase A₂ and secretion of CCK compared to control group. The results of this study indicate that dietary supplementation 2% chicken egg lecithin indirectly through some biochemical parameters improves growth performance of rainbow trout fry.     

Effects of different commercial diets on growth performance,health and resistance to Tetrahymena sp. infection in guppies,Poecilia reticulata (Peters)

The effects of feeding guppy fry, Poecilia reticulata (Peters), different commercial diets from BernAqua [MeM (R = Regular, O = Ornamental and P = Premium) and experimental feed (EF] and Ocean Nutrition (ON) on growth was tested for 57 days. Health status and resistance to Tetrahymena infection were analysed at the end of the trial. The highest growth was obtained in fish fed MeM Regular and MeM Premium (mean final weights of 427 and 417 mg respectively). Fish fed EF had a significantly higher rate of spinal deformity (12.7%) than all other feeding groups (3.05% or less). Histological analysis revealed the accumulation of liver glycogen and/or lipid in fish from all feeding groups; fat accumulation in the abdomen was most pronounced in the EF and MeM Regular‐fed fish, and muscle dystrophy was observed in ca. 50% of the fish from all feeding groups except the MeM Ornamental‐fed group. The highest mortality following Tetrahymena infection occurred in fish fed EF (87%) and the lowest mortality in the MeM Ornamental‐fed fish (58%). Mortalities in all other feeding groups ranged between 69% and 76%. In summary, MeM Ornamental feed provided a moderate weight gain, no muscle dystrophy, negligible deformity and the greatest resistance to parasitic infection.     

Effect of temperature on growth and survival of maraena whitefish Coregonus maraena (Bloch 1779) larvae in controlled conditions

This 28‐day study investigated the effect of three rearing temperatures, 11, 15 and 19°C, on survival and growth of maraena whitefish fry in a recirculating aquaculture system. Three groups of larvae in three repetitions were reared in recirculating system. Each group comprised 200 larvae. Feeding level was fixed at 500–700 Artemia sp. metanauplii per fish per day. Larvae were fed fresh live brine shrimp at 10 ml/tank every 3 hr. Significantly higher body weight (p = 0.00), total length (p = 0.00), larval yield (p = 0.00) and condition factor (p = 0.00) were obtained at 19°C compared to 15 and 11°C, as well as at 15°C compared to 11°C. Significantly higher survival (p = 0.00) was observed in larvae reared at 11 and 15°C compare to 19°C and no significant differences were observed between 11°C compared to 15°C. No significant differences in size heterogeneity among treatments were found (p = 0.46). In larviculture, the optimal assessed temperature for growth of maraena whitefish was 19°C, with highest survival observed at 11°C, at the end of this 28 days trial. The findings in this study apply to the particular study location and may not be applicable more broadly.     

Genetic parameters for resistance against Flavobacterium columnare in Nile tilapia Oreochromis niloticus (Linnaeus, 1758)

Columnaris disease is a major cause of mortality in tilapia hatcheries and commonly occurs during the summer season in Thailand. One way of reducing the problem is by selective breeding for increased disease resistance. The objective of this study was to estimate quantitative genetic parameters for resistance against columnaris in the Chitralada 4 strain of Nile tilapia. Data from 43 full‐sib families (2,580 records) of fry (age = 32 ± 4 days post‐hatch) were used in the analyses. Initially, fry were subjected to bath challenge with Flavobacterium columnare (LD₅₀ concentration = 1.2 × 10⁶ CFU/ml) for 14 days. Disease resistance was defined as the number of days from challenge until death (DD) or as a binary trait (dead/alive) on day 14. Linear animal and sire‐dam models were used for DD, while threshold animal, threshold sire‐dam, binary linear animal and binary linear sire‐dam models were used for binary outcomes. Covariate effect of age, fixed effect of challenge day and random effects of the individual animals or sires and dams were included in the models. Mean survival was 32.4 ± 11.6%, and survival rates of the best and poorest families were 70% and 8%, respectively. The highest estimate of heritability (0.30 ± 0.025) was obtained under the threshold sire‐dam model. Heritability estimates for DD (0.16 ± 0.034 and 0.17 ± 0.046) were comparable to those obtained from the threshold animal (0.15 ± 0.031) and the binary linear (0.14 ± 0.045 and 0.15 ± 0.044) models. The linear animal and sire‐dam models for DD and the threshold sire‐dam models performed equally with similar values of r_EBV (0.629, 0.628 and 0.627) and accuracy of selection (0.793, 0.793 and 0.791). This study reveals the potential of selective breeding to increase disease resistance to F. columnare in the studied population of Nile tilapia.     

High density rearing of rohu,Labeo rohita (Hamilton) from spawn to fry in concrete tanks: effect of daily feed ration and soil base on fry growth and survival

Rearing of rohu spawn to fry size was carried out at high density (2000 spawn m^?2) in large concrete nursery tanks (10 m × 5 m × 1.2 m) to standardize the daily ration of supplementary feed and to assess the impact of soil base on the fry performance. In Set‐1, supplementary feed was provided at 100% (control), 75% and 50% of the daily ration that is commonly used in earthen carp nursery ponds and named as the three treatments T‐1, T‐2 and T‐3 respectively. Although in Set‐II, performance of fry were evaluated in tanks provided with and without soil base. The fry survival was to the tune of 45–59% at this high density of 2000 spawn m^?2, indicating the efficacy of the concrete nursery system for high density seed rearing. In Set‐I, T‐2 receiving 75% of the control ration exhibited similar fry growth as that of T‐1, but its fry survival (58.8%) was 10% higher than the latter. This indicated that supplementary feed in a concrete nursery can be reduced up to 25% of that provided in earthen nursery system while ensuring a higher fry yield. However, further reduction up to 50% of the control ration in T‐3 yielded lower fry survival and growth (P < 0.05) revealing inadequacy of the available feed. In Set‐II, treatment without soil base (T‐ns) yielded similar fry growth but 10% higher survival compared to that with the soil base (T‐s) revealing non‐essentiality of soil base in concrete tanks for such short‐term nursery rearing of rohu.     

Diphenyl diselenide‐loaded nanocapsules in silver catfish feed enhance growth,improve muscle antioxidant/oxidant status and increase selenium deposition: Advantages of nanotechnology for fish health

The aim of this study was to determine whether feed supplementation with nanoencapsulated Ph₂Se₂ would improve the growth performance of silver catfish and would maintain or improve the muscle antioxidant system. Final mean weight (p = .031), weight gain (p = .014) and specific growth rates (p = .045) were significantly higher in supplemented fish than in the control group. Muscle reactive oxygen species (p = .0001) levels were significantly lower in supplemented fish than in the control group. Also, muscle hydrogen peroxide (p = .0001) and lipid peroxidation (p = .0001) levels were significantly lower in supplemented fish than in the control group. Muscle antioxidant capacity against peroxyl radical (p = .00001) levels were significantly higher in fish supplemented with both Ph₂Se₂ forms (free or nanocapsules) than in the control group, while muscle superoxide dismutase (p = .001) and catalase (p = .0001) activities were significantly higher in supplemented fish than in the control group. Finally, selenium (Se) deposition (p = .00001) was significantly higher in the muscle of fish supplemented with free and nanoencapsulated forms compared with the control; and deposition levels were significantly higher in fish supplemented with Ph₂Se₂‐loaded in nanocapsules compared with the free form. Based on this evidence, Ph₂Se₂‐loaded in nanocapsules improves silver catfish performance and antioxidant/oxidant status. The greater content of Se (1.22 µg/g muscle) in the muscle of fish fed with dietary Ph₂Se₂‐loaded nanocapsules indicates that it may be considered a nutraceutical for fish.     

Effects of different natural or prepared diets on growth and survival of juvenile spider crabs, <Emphasis Type="Italic">Maja brachydactyla</Emphasis> (Balss, 1922)

The effects of different diets (natural or pellets) on growth, survival, and moulting interval of juvenile spider crabs, weighing between 0.011–1.56 g and up to 17.6 mm in carapace length, were tested over a period of 90 days. During experiment I, five diets were tested: (1) frozen shrimp—Paleomonetes sp., (2) fresh mussels—Mytilus sp., (3) white fish fillets—Merlucius merlucius, (4) blue fish fillets—Sardina pilchardus, and (5) commercial crustacean pellets. Spider crabs fed fresh mussels grew larger (0.98 ± 0.69 g) and had higher growth rates (4.0 ± 0.7 %BWd⁻¹) compared to the other four diets. The crabs fed shrimp pellet and frozen shrimp grew to intermediate sizes and were smaller than the ones fed fresh mussels, but they were larger than spider crabs fed either blue or white fish fillets (0.46 ± 0.63 and 0.26 ± 0.13 g, respectively) compared to the ones fed white fish fillets (0.12 ± 0.04) and blue fish fillets (0.04 ± 0.02 g). The spider crabs fed blue fish fillets only lasted until day 60 of the experiment, after this day none of the 20 fed this diet were left. During experiment II, two diets were tested: (1) white and blue fish fillets and (2) commercial fish pellet. There were no differences in growth both in weight or carapace length (2.9 ± 1.8 and 2.1 ± 1.5 g in weight, and 18.9 ± 5.0 and 17.7 ± 3.3 mm, respectively) at the end of the experiment. Similarly, there were no differences in growth rates in weight between the two diets (1.2 ± 0.4 and 0.9 ± 0.3 %BWd⁻¹, respectively) or in carapace length (0.4 ± 0.1 and 0.4 ± 0.2 %BWd⁻¹, respectively). Fresh mussel appears to be a very good diet to culture the early stages of this species, while shrimp pellets also deliver acceptable results. On the contrary, frozen shrimp, fish fillets either from blue or white species (much higher lipid content in the blue species), and fish pellets were found to be bad diets for the culture of the early stages of M. brachydactyla.     

Nano zinc vis‐à‐vis inorganic Zinc as feed additives: Effects on growth,activity of hepatic enzymes and non‐specific immunity in rohu,Labeo rohita (Hamilton) fingerlings

The effects of dietary administration of inorganic zinc (zinc sulphate, ZnSO₄) and nano zinc (zinc oxide nanoparticles, ZnO‐NP) were evaluated in rohu, Labeo rohita fingerlings. Fish were fed with a basal diet (Control) supplemented with ZnSO₄ (T1, T2 and T3) and ZnO‐NP (T4, T5 and T6) at 10, 20 and 30 mg/kg, respectively, for a duration of 45 days. The results revealed that fish fed diet containing 20 mg ZnO‐NP per kg (T5) had the highest weight gain and specific growth rate (SGR, % per day), which was significantly different (p < .05) from the other experimental diets. Significantly (p < .05), higher activities of the digestive and metabolic enzymes were recorded in the fish fed ZnO‐NP containing diets as compared to the diets containing inorganic Zn or control diet. The maximum serum glucose and protein levels were noted in fish reared on diet T5. Both SGOT and SGPT activities were significantly increased in fish fed Zn‐supplemented diets (T1 to T6), as compared to the control group. Similarly, innate immune parameters were improved with feeding Zn incorporated diets. The highest phagocytic (40.74 ± 0.65%) and respiratory burst (0.33 ± 0.001, OD 630nm) activities were recorded in the fish fed diet containing ZnO‐NPs at 20 mg/kg (T5). The maximum superoxide production and serum peroxidase activity were detected in the fish fed T5 and T6 diets. Overall, results indicated that short‐duration feeding (≤45 days) of dietary ZnO‐NP (20 mg/kg) improved growth, enzyme activity, serum biochemical parameters and immune function in rohu fingerlings.     

Determination of grapefruit (Citrus paradisi) peel extract bio‐active substances and its application in Caspian white fish (Rutilus frisii kutum) diet: Growth,haemato‐biochemical parameters and intestinal morphology

In this study, total phenolic and flavonoid contents of grapefruit peel extract (GPE) were equal to 117.3 ± 0.3 µg of gallic acid/mg and 39.30 ± 0.1 µg of quercetin/mg respectively. Caspian white fish (n = 180, 4 ± 0.9 g body weight) was fed with supplemented diets, including 0, 6.25, 12.5 and 25 mg of GPE/kg for 60 days at 25 ± 1°C. The growth performance was markedly improved in fish fed with 25 mg/kg of GPE compared to others (p < 0.05). Moreover, fish fed with 25 mg/kg of GPE showed a significant increase in red blood cell (2.65·10⁶ cell/mm³), white blood cell (17.75·10³ cell/mm³), packed cell volume (48%) and haemoglobin concentrations (8.75 g/dl) compared to the control (p < 0.05). However, the highest alanine aminotransferase (140 U/L), alanine transaminase (14.5 U/L), and alkaline phosphatase (18.5 U/L) were observed in control group. Morphological analysis of intestine revealed the highest amount of villus width (8.4 µM), height (32.86 µM) and surface area (342.7 µM²) in fish fed with 25 mg/kg of GPE (p < 0.05). In conclusion, supplementing feed with GPE at 25 g/kg can improve growth performance and haemato‐biochemical parameters of Caspian white fish fry.     

Growth and survival of hatchery-bred giant clams (<Emphasis Type="Italic">Tridacna gigas</Emphasis>) in an ocean nursery in Sagay Marine Reserve,Philippines

To restore the diminishing population of the giant clam Tridacna gigas in Sagay Marine Reserve (SMR), Negros Occidental, central Philippines, two size classes [8- and 10-cm shell length (SL)] of hatchery-bred T. gigas were reared in an adjacent ocean nursery for restocking to Carbin Reef later upon reaching grow-out size of ≥20 cm SL. Growth rates did not significantly differ for both sizes and were on average 0.67 cm month⁻¹. However, survival after 382 days of rearing T. gigas was significantly higher in the 10-cm SL clams than the 8-cm SL clams (96 and 83%, respectively). For future restocking projects, the use of 8-cm SL clams is recommended because the lower survival of this size class is compensated by its cheaper price. While rearing the clams to attain grow-out size, the population of wild clams (Family Tridacnidae) in Carbin Reef was assessed using ten 50 × 2-m belt transects. Four species of tridacnid clams have been recorded: Hippopus hippopus, Tridacna crocea, T. maxima, and T. squamosa. T. crocea comprised 12.5–93.9% of all the clams observed in all ten transects. There was a significant difference in clam density between species (ANOVA, F = 6.94, P < 0.001), with T. crocea having the highest density. Living T. gigas were absent, but presence of dead shells was indicative of its presence in the reef in the past. It can be expected that the release of hatchery-bred T. gigas juveniles in Carbin Reef could provide future breeders that will repopulate this reef and the adjacent reef communities.     

Modulation of key enzymes of glycolysis, gluconeogenesis, amino acid catabolism, and TCA cycle of the tropical freshwater fish Labeo rohita fed gelatinized and non-gelatinized starch diet

A 60-day experiment was conducted to study the effect of dietary gelatinized (G) and non-gelatinized (NG) starch on the key metabolic enzymes of glycolysis (hexokinase, glucokinase, pyruvate kinase, and lactate dehydrogenase), gluconeogenesis (glucose-6 phosphatase and fructose-1,6 bisphosphatase), protein metabolism (aspartate amino transferase and alanine amino transferase), and TCA cycle (malate dehydrogenase) in Labeo rohita juveniles. In the analysis, 234 juveniles (2.53 ± 0.04 g) were randomly distributed into six treatment groups each with three replicates. Six semi-purified diets containing NG and G cornstarch, each at six levels of inclusion (0, 20, 40, 60, 80, and 100) were prepared viz., T1 (100% NG, 0% G starch), T2 (80% NG, 20% G starch), T3 (60% NG, 40% G starch), T4 (40% NG, 60% G starch), T5 (20% NG, 80% G starch), and T6 (0% NG, 100% G starch). Dietary G:NG starch ratio had a significant (P < 0.05) effect on the glycolytic enzymes, the highest activities were observed in the T6 group and lowest in the T1 group. On the contrary, the gluconeogenic enzymes, the glucose-6-phosphatase and fructose-1,6 bisphosphatase activities in the organs, liver and kidney were recorded highest in the T1 group and lowest in the T6 group. The liver aspartate amino transferase activity showed an increasing trend with the decrease in the dietary G level. However, the muscle aspartate amino transferase activity was not significantly (P > 0.05) influenced by the type of dietary starch. The alanine amino transferase activity in both liver and muscle showed an increasing trend with the decrease in the dietary G level. The liver and muscle malate dehydrogenase activities were lowest in the T6 group and highest in the T1 group. Results suggest that NG (100%) starch diet significantly induced more the enzyme activities of amino acid metabolism, gluconeogenesis, and TCA cycle, whereas partial or total replacement of raw starch by gelatinized starch increased the glycolytic enzyme activity.